Rmu_sc0019347.1_g000001

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0019347.1
OG0000067
Physical Location & Seq
Reverse (-)
5979 .. 10952
4974 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0019347.1_g000001.1.cds

Sequence Viewer

Length: 4047 bp
atggccgcttcttcatcttcaccttcttctggtggtcattgcaagtatgatgtcttcctcagttttcgaggggaggacactcgcaaaaacttcgtttgccatctttacaaagctctaggacagaaagcaatctacaccttcatcgattctgaagagctcgagaaaggcaacaaaatttccgagctactcaacgctgttgccgaatcgaggatttcggttgtagttttatctaaaaactatgccacttccacttggtgcttaaaagagctcgtcaaaatcatggaatgcgaggataagaagcagcagattgtggtgcccattttctatgatgtagatccctccgatattcgaaaattgaagaaaatgtttggggaagcttttgccaaacatgaacaaaatcctaacctcgacaaggaagaattggagacctggaaagctgcactaaaaggaatcacggatctatctggctgggattcgctacggtataaggatgacatcgagcttattgagaaaattgtaggagacatattagaaaaatggattcgcatccccggaccatccagtctagcaaacaattttgttggaatggatcgtcacattaagaaagtgagcttgctgttgtgtcgtgggaagaatgatgttggtgttgttggaatatggggtatgggtggtataggcaaaacaaccatagcaagagctgtttatgacaaaatcgctcatcaatttgaacacaagtgttttttttataacgtgaaagaacgtttagcgaagaaggatgaagcacaaatgcggggagaacttctatctatgatcttgaaggggaatgagcagtgtgtagacattttcaatcaaggttccaatgcaatactggaaaggctaggtaagaaaaaaattctagttgttcttgatgatgtggaaacgttttcccaaattgaagccctacttggagatttttattcatttggtgttggaagtagaatccttgtaacaactagagataaggaatcactagctggagtttatgagacatatgatcccaagtgtttaaattataatgaatctcttgaactcttcatgaagcatgccttcagaacaaaccaacccagtaaagggtatagttacctctcaaaccgagccatagagtatgctcagggtctgcctttagcacttaaagtcttgggagcttctctttataacaagagtatacaccagtggagagaggtgttagagaaaatcaagaaaatcccgcaaaggaagattctggatgtgcttagaacaagcttggagggactagacgatctagagaaagacatatttctagacatcgcatgtttctttagagaatgggatgtatatgttgtaacagaattactagacagttgtgaatctactaaagaacctgggaagcgcagtaggttatggagttttgaagatgttaattatgtattgactcaaaatacggcgacggatgcagttgaatgcttaatcctagacttgtcgaactcaaaagtcaatctatgcatacatactggagcttttgttagaatgacgaagctaagactactcatgattttttattcattctccaatataaatgactaccccaattatttcaacgatgacaactacctcaattatatcgatgacgatgccggtgaattctattttaaatatagctgtgagcgtttatgtcctgttgatggctgtaaacaactctggatgggggacttggagtatctttctcaagaattgaggtttctcttatggcatggatgcccgctaaagtctttgccatcaacctttatcccgaaaaatcttgttcaacttgacatgcgtggtagtcacattcaacaactttgggaaggaatcaagcccctgccaaatctgaaagtcatcttgttaagtgactgtcaatacattgtcaaaatccctgacttaactgaggcgataaattttgtgagactatttcttgatggctgttcaagtttaattgagtttgagtttcattcatccatttatgctcttcaaaaccttcatttcttggatctaaaggggtgcaagcaacttaagattctgccaagctgcattcatatgaaatctctacggatccttcgtctatctggctgttcaaatctcgaaaagtttccagagatttcagaggttatgatgaagctaaaatggctttatttagatgggactgcaattaaagaactgccctcatctattaacaatctcactggaattgttactttagatctgaaaggttgcacagaacttaagactctaccaatcagcattcatatgcgttctcttcagacccttaatctcgatggctgctcaaatcttgaaaagtttccggagatttcaggaatcatgaaggagctagtagtgcttggtttagatgagaccgcaattaaaggattaccctcatcaattgatctgcttgtggggcttaaacacttaactatgcggaattgcaaaagccttgtctctcttcctgacagtatctcacttcataacttgcctgagaaattgggagacttagaatcattagaggacctggacgtagatgatagtggtgtaaaacaacttccattctctatccttcgtgtgtgttctgtattatcatgtattgggtgtaaagagatgacggcacccttttcagcgtggccctcatcagtggaagaatatcgtagttactctgttctgctacatcttgatttaagtgactgcaatctgttggagttatcagatggtattgctcatttatcttcattggtaacattaaagctgtgcagaaacaatttggagagcttacctgtgacaatgaatcaacttggttggtcaacgcatcttgaattggaagcttgcaagaggctgaaatcaataccagagctttcatcgagtataaattacatagatgcacatgattgcacagctttggaaacagtttcaacaccaaaaactagaaatcttgacttcagattttctaattgcttccggctggtacaagcaaatttgtttagagatattgttgaaattcattctctcgaccaggataatcatctgcgacctctgtgcttcaacatgtgtcttcctggaagtgaagttccagaatggttcaaccttcgggatacgggatgttcagtaactttccatgttccactgaatagtttcaatagcaagcttttaggatttgctacatgcattgttattaacttcagtgatttggttggccggcagtacagaactcacagatttctagagtcagatcacctgttcatgggatatatgtcatggtctgaatatggctggattgcaaaacatagattagatgcatttacgattggggtagaaactggtacatattttccaacacacggttgctttactagtagctgcggtgttcgtttagtttatgctaatcacgaggagatgggaaatagcgtgacacagcccttaattcctgtggatagccgggaagggggagtggatggccagagaatggaggatgcggtgcagcagggggaggattcctgtgctaaaggaggggtgttagggcttggagcaccgttttttccgaaccagctggaaataagagagagtgaaacagagtgggaggcagagtcatcagagtcgggtgaagggagaggcaggtatcaaacattggagatgttattagagccacagatggagggtaaaaaagaggagataatggtggtggtggagcagggggaggggtgccaggtgttagggtcggcattctctttgagaaagagagaaacagaaagagaaggagacagagtcaccagagtctctgatgatggggagatatcggagttgggcagccacagtggtctggtggtgccaaattcacagagtgagggcaaagaagagaaagaattgattggggacgagtcgttggctggaagagggttgttagggccactatgcagctgcttctactggctgggtgaagtatggcgtggaaagagagaagtaccagaagcagcggagtttggcttgcatgaaaggaaaaaagaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1348

Amino Acids

152.32

Weight (kDa)

5.41

Isoelectric Point (pI)

38.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 747, 1053, 1194
AasI GACNNNNNNGTC 1 cut(s) 561
Acc36I ACCTGC 1 cut(s) 3645
AccB1I GGYRCC 4 cut(s) 313, 2653, 3741, 3865
AccB7I CCANNNNNTGG 1 cut(s) 3505
AccI GTMKAC 2 cut(s) 837, 1204
AccIII TCCGGA 1 cut(s) 2356
AciI CCGC 9 cut(s) 6, 790, 1247, 1778, 2409, 2470, 3402, 3515, 4013
AclI AACGTT 2 cut(s) 760, 920
AclWI GGATC 7 cut(s) 329, 465, 597, 1028, 2052, 2101, 2114
AcoI YGGCCR 3 cut(s) 3, 3235, 3496
AcsI RAATTY 7 cut(s) 174, 891, 1658, 1951, 3016, 3039, 3871
AcuI CTGAAG 5 cut(s) 171, 1072, 2297, 2965, 3205
AdeI CACNNNGTG 2 cut(s) 255, 3881
AfaI GTAC 4 cut(s) 3009, 3245, 3364, 4002
AflII CTTAAG 2 cut(s) 2066, 2276
AflIII ACRYGT 1 cut(s) 3087
AhlI ACTAGT 1 cut(s) 3392
AjnI CCWGG 6 cut(s) 428, 1399, 2559, 3054, 3097, 3744
AjuI GAANNNNNNNTTGG 2 cut(s) 927, 959
AloI GAACNNNNNNTCC 2 cut(s) 3093, 3125
Alw21I GWGCWC 3 cut(s) 159, 270, 3571
Alw26I GTCTC 9 cut(s) 419, 516, 1019, 1954, 2399, 2494, 2532, 3792, 3820
AlwI GGATC 7 cut(s) 329, 465, 597, 1028, 2052, 2101, 2114
AlwNI CAGNNNCTG 1 cut(s) 1156
Ama87I CYCGRG 1 cut(s) 158
Aor13HI TCCGGA 1 cut(s) 2356
AoxI GGCC 5 cut(s) 3, 2669, 3235, 3496, 3944
ApoI RAATTY 7 cut(s) 174, 891, 1658, 1951, 3016, 3039, 3871
ArsI GACNNNNNNTTYG 2 cut(s) 2472, 2504
Asp700I GAANNNNTTC 3 cut(s) 2142, 2352, 3173
AspLEI GCGC 1 cut(s) 1410
AspS9I GGNCC 4 cut(s) 554, 2557, 2670, 3944
AsuC2I CCSGG 2 cut(s) 552, 3479
AsuHPI GGTGA 6 cut(s) 12, 1667, 3266, 3653, 3799, 3986
AsuII TTCGAA 1 cut(s) 349
AvaI CYCGRG 1 cut(s) 158
AvaII GGWCC 2 cut(s) 554, 2557
BaeGI GKGCMC 1 cut(s) 318
BalI TGGCCA 1 cut(s) 3498
BamHI GGATCC 1 cut(s) 2106
BanI GGYRCC 4 cut(s) 313, 2653, 3741, 3865
BanII GRGCYC 2 cut(s) 159, 270
BarI GAAGNNNNNNTAC 4 cut(s) 38, 70, 2574, 2606
BauI CACGAG 1 cut(s) 3428
BbsI GAAGAC 2 cut(s) 46, 3086
Bbv12I GWGCWC 3 cut(s) 159, 270, 3571
BceAI ACGGC 2 cut(s) 1476, 2667
BcgI CGANNNNNNTGC 6 cut(s) 73, 107, 1631, 1665, 3060, 3094
BciT130I CCWGG 6 cut(s) 430, 1401, 2561, 3056, 3099, 3746
BciVI GTATCC 1 cut(s) 3127
BcnI CCSGG 2 cut(s) 552, 3479
BcoDI GTCTC 9 cut(s) 419, 516, 1019, 1954, 2399, 2494, 2532, 3792, 3820
BcuI ACTAGT 1 cut(s) 3392
BfrI CTTAAG 2 cut(s) 2066, 2276
BfuAI ACCTGC 1 cut(s) 3645
BfuI GTATCC 1 cut(s) 3127
BglII AGATCT 1 cut(s) 2254
Bme1390I CCNGG 8 cut(s) 430, 552, 1401, 2561, 3056, 3099, 3479, 3746
Bme18I GGWCC 2 cut(s) 554, 2557
BmeT110I CYCGRG 1 cut(s) 158
BmgT120I GGNCC 4 cut(s) 554, 2557, 2670, 3944
BmiI GGNNCC 6 cut(s) 315, 856, 2108, 2655, 3743, 3867
BmrFI CCNGG 8 cut(s) 430, 552, 1401, 2561, 3056, 3099, 3479, 3746
BmrI ACTGGG 1 cut(s) 1098
BmsI GCATC 8 cut(s) 555, 1459, 1639, 1763, 2860, 2911, 3325, 3502
BmuI ACTGGG 1 cut(s) 1098
BpiI GAAGAC 2 cut(s) 46, 3086
BpmI CTGGAG 2 cut(s) 1035, 1551
Bpu10I CCTNAGC 1 cut(s) 1149
Bpu14I TTCGAA 1 cut(s) 349
BpuEI CTTGAG 1 cut(s) 1728
BpuMI CCSGG 2 cut(s) 552, 3479
Bsa29I ATCGAT 2 cut(s) 144, 1641
BsaBI GATNNNNATC 3 cut(s) 333, 545, 3063
BsaI GGTCTC 2 cut(s) 419, 2399
BsaJI CCNNGG 2 cut(s) 550, 1400
BsaWI WCCGGW 1 cut(s) 2356
BsaXI ACNNNNNCTCC 6 cut(s) 1524, 1554, 3611, 3641, 3789, 3819
Bse118I RCCGGY 2 cut(s) 1652, 3237
Bse1I ACTGG 8 cut(s) 561, 873, 1104, 1210, 1534, 2242, 3364, 3971
Bse3DI GCAATG 1 cut(s) 37
Bse8I GATNNNNATC 3 cut(s) 333, 545, 3063
BseAI TCCGGA 1 cut(s) 2356
BseBI CCWGG 6 cut(s) 430, 1401, 2561, 3056, 3099, 3746
BseCI ATCGAT 2 cut(s) 144, 1641
BseDI CCNNGG 2 cut(s) 550, 1400
BseJI GATNNNNATC 3 cut(s) 333, 545, 3063
BseMI GCAATG 1 cut(s) 37
BseMII CTCAG 4 cut(s) 73, 1163, 1932, 2517
BseNI ACTGG 8 cut(s) 561, 873, 1104, 1210, 1534, 2242, 3364, 3971
BseRI GAGGAG 2 cut(s) 3446, 3722
BseSI GKGCMC 1 cut(s) 318
BseYI CCCAGC 2 cut(s) 468, 3970
BsgI GTGCAG 3 cut(s) 423, 2815, 3539
BshFI GGCC 5 cut(s) 5, 2671, 3237, 3498, 3946
BshNI GGYRCC 4 cut(s) 313, 2653, 3741, 3865
BshVI ATCGAT 2 cut(s) 144, 1641
BsiHKAI GWGCWC 3 cut(s) 159, 270, 3571
BsiHKCI CYCGRG 1 cut(s) 158
BsiSI CCGG 6 cut(s) 552, 1653, 2357, 3001, 3238, 3478
BslFI GGGAC 4 cut(s) 1302, 1739, 2209, 3926
BsmAI GTCTC 9 cut(s) 419, 516, 1019, 1954, 2399, 2494, 2532, 3792, 3820
BsmFI GGGAC 4 cut(s) 1302, 1739, 2209, 3926
BsmI GAATGC 5 cut(s) 290, 1484, 2085, 2295, 3761
BsnI GGCC 5 cut(s) 5, 2671, 3237, 3498, 3946
Bso31I GGTCTC 2 cut(s) 419, 2399
BsoBI CYCGRG 1 cut(s) 158
Bsp119I TTCGAA 1 cut(s) 349
Bsp1286I GDGCHC 4 cut(s) 159, 270, 318, 3571
Bsp13I TCCGGA 1 cut(s) 2356
BspACI CCGC 9 cut(s) 6, 790, 1247, 1778, 2409, 2470, 3402, 3515, 4013
BspANI GGCC 5 cut(s) 5, 2671, 3237, 3498, 3946
BspCNI CTCAG 4 cut(s) 72, 1162, 1933, 2518
BspDI ATCGAT 2 cut(s) 144, 1641
BspEI TCCGGA 1 cut(s) 2356
BspHI TCATGA 3 cut(s) 1074, 1566, 2373
BspLI GGNNCC 6 cut(s) 315, 856, 2108, 2655, 3743, 3867
BspMI ACCTGC 1 cut(s) 3645
BspPI GGATC 7 cut(s) 329, 465, 597, 1028, 2052, 2101, 2114
BspQI GCTCTTC 2 cut(s) 147, 2028
BspT104I TTCGAA 1 cut(s) 349
BspT107I GGYRCC 4 cut(s) 313, 2653, 3741, 3865
BspTI CTTAAG 2 cut(s) 2066, 2276
BspTNI GGTCTC 2 cut(s) 419, 2399
BsrDI GCAATG 1 cut(s) 37
BsrFI RCCGGY 2 cut(s) 1652, 3237
BsrI ACTGG 8 cut(s) 561, 873, 1104, 1210, 1534, 2242, 3364, 3971
BssAI RCCGGY 2 cut(s) 1652, 3237
BssECI CCNNGG 2 cut(s) 550, 1400
BssNAI GTATAC 1 cut(s) 1205
BssSI CACGAG 1 cut(s) 3428
Bst1107I GTATAC 1 cut(s) 1205
Bst2BI CACGAG 1 cut(s) 3428
Bst2UI CCWGG 6 cut(s) 430, 1401, 2561, 3056, 3099, 3746
Bst4CI ACNGT 8 cut(s) 483, 1379, 1910, 2504, 2950, 3383, 3573, 3854
Bst6I CTCTTC 7 cut(s) 147, 1076, 2028, 2316, 2499, 3888, 3925
BstAFI CTTAAG 2 cut(s) 2066, 2276
BstBI TTCGAA 1 cut(s) 349
BstC8I GCNNGC 8 cut(s) 614, 1083, 1778, 2060, 2869, 3185, 3239, 4025
BstDEI CTNAG 7 cut(s) 59, 1149, 1271, 1556, 1941, 2526, 2542
BstENI CCTNNNNNAGG 1 cut(s) 410
BstHHI GCGC 1 cut(s) 1410
BstMAI GTCTC 9 cut(s) 419, 516, 1019, 1954, 2399, 2494, 2532, 3792, 3820
BstMWI GCNNNNNNNGC 4 cut(s) 1469, 2179, 2389, 2449
BstNI CCWGG 6 cut(s) 430, 1401, 2561, 3056, 3099, 3746
BstNSI RCATGY 5 cut(s) 1085, 1332, 1834, 3091, 3207
BstSCI CCNGG 8 cut(s) 428, 550, 1399, 2559, 3054, 3097, 3477, 3744
BstSLI GKGCMC 1 cut(s) 318
BstV2I GAAGAC 2 cut(s) 46, 3086
BstX2I RGATCY 5 cut(s) 334, 457, 2044, 2106, 2254
BstYI RGATCY 5 cut(s) 334, 457, 2044, 2106, 2254
BstZ17I GTATAC 1 cut(s) 1205
Bsu15I ATCGAT 2 cut(s) 144, 1641
BsuI GTATCC 1 cut(s) 3127
BsuRI GGCC 5 cut(s) 5, 2671, 3237, 3498, 3946
BsuTUI ATCGAT 2 cut(s) 144, 1641
BtgZI GCGATG 1 cut(s) 1309
BtsI GCAGTG 1 cut(s) 836
BtsIMutI CAGTG 7 cut(s) 836, 1217, 2235, 2685, 3164, 3229, 3859
BveI ACCTGC 1 cut(s) 3645
Cac8I GCNNGC 8 cut(s) 614, 1083, 1778, 2060, 2869, 3185, 3239, 4025
CaiI CAGNNNCTG 1 cut(s) 1156
CciI TCATGA 3 cut(s) 1074, 1566, 2373
CfoI GCGC 1 cut(s) 1410
Cfr10I RCCGGY 2 cut(s) 1652, 3237
Cfr13I GGNCC 4 cut(s) 554, 2557, 2670, 3944
ClaI ATCGAT 2 cut(s) 144, 1641
Csp6I GTAC 4 cut(s) 3008, 3244, 3363, 4001
CviQI GTAC 4 cut(s) 3008, 3244, 3363, 4001
DdeI CTNAG 7 cut(s) 59, 1149, 1271, 1556, 1941, 2526, 2542
DraI TTTAAA 2 cut(s) 1047, 1669
DraIII CACNNNGTG 2 cut(s) 255, 3881
DrdI GACNNNNNNGTC 1 cut(s) 561
DseDI GACNNNNNNGTC 1 cut(s) 561
EaeI YGGCCR 3 cut(s) 3, 3235, 3496
Eam1104I CTCTTC 7 cut(s) 147, 1076, 2028, 2316, 2499, 3888, 3925
EarI CTCTTC 7 cut(s) 147, 1076, 2028, 2316, 2499, 3888, 3925
Ecl136II GAGCTC 2 cut(s) 157, 268
Eco24I GRGCYC 2 cut(s) 159, 270
Eco31I GGTCTC 2 cut(s) 419, 2399
Eco32I GATATC 1 cut(s) 3834
Eco47I GGWCC 2 cut(s) 554, 2557
Eco53kI GAGCTC 2 cut(s) 157, 268
Eco57I CTGAAG 5 cut(s) 171, 1072, 2297, 2965, 3205
Eco88I CYCGRG 1 cut(s) 158
EcoICRI GAGCTC 2 cut(s) 157, 268
EcoNI CCTNNNNNAGG 1 cut(s) 410
EcoO109I RGGNCCY 1 cut(s) 2557
EcoRI GAATTC 1 cut(s) 1658
EcoRII CCWGG 6 cut(s) 428, 1399, 2559, 3054, 3097, 3744
EcoRV GATATC 1 cut(s) 3834
EcoT22I ATGCAT 3 cut(s) 1523, 3209, 3340
EcoT38I GRGCYC 2 cut(s) 159, 270
FalI AAGNNNNNCTT 4 cut(s) 927, 959, 1070, 1102
FaqI GGGAC 4 cut(s) 1302, 1739, 2209, 3926
FauI CCCGC 3 cut(s) 783, 1254, 1785
FauNDI CATATG 3 cut(s) 1030, 2091, 2301
FblI GTMKAC 2 cut(s) 837, 1204
FriOI GRGCYC 2 cut(s) 159, 270
GlaI GCGC 1 cut(s) 1409
GsaI CCCAGC 2 cut(s) 472, 3974
GsuI CTGGAG 2 cut(s) 1035, 1551
HaeIII GGCC 5 cut(s) 5, 2671, 3237, 3498, 3946
HapII CCGG 6 cut(s) 552, 1653, 2357, 3001, 3238, 3478
HhaI GCGC 1 cut(s) 1410
Hin6I GCGC 1 cut(s) 1408
HinP1I GCGC 1 cut(s) 1408
HincII GTYRAC 1 cut(s) 2847
HindII GTYRAC 1 cut(s) 2847
HindIII AAGCTT 4 cut(s) 375, 1279, 2865, 3185
HpaII CCGG 6 cut(s) 552, 1653, 2357, 3001, 3238, 3478
HphI GGTGA 6 cut(s) 12, 1667, 3266, 3653, 3799, 3986
Hpy166II GTNNAC 4 cut(s) 838, 1205, 1709, 2847
Hpy8I GTNNAC 4 cut(s) 838, 1205, 1709, 2847
Hpy99I CGWCG 1 cut(s) 1468
HpyCH4III ACNGT 8 cut(s) 483, 1379, 1910, 2504, 2950, 3383, 3573, 3854
HpyCH4IV ACGT 4 cut(s) 750, 760, 920, 2565
HpyF10VI GCNNNNNNNGC 4 cut(s) 1469, 2179, 2389, 2449
HpyF3I CTNAG 7 cut(s) 59, 1149, 1271, 1556, 1941, 2526, 2542
HpySE526I ACGT 4 cut(s) 750, 760, 920, 2565
HspAI GCGC 1 cut(s) 1408
Kpn2I TCCGGA 1 cut(s) 2356
KroI GCCGGC 1 cut(s) 3237
KroNI GCCGGC 1 cut(s) 3239
LguI GCTCTTC 2 cut(s) 147, 2028
LmnI GCTCC 5 cut(s) 1181, 1532, 2380, 3566, 3727
LweI GCATC 8 cut(s) 555, 1459, 1639, 1763, 2860, 2911, 3325, 3502
MaeII ACGT 4 cut(s) 750, 760, 920, 2565
MfeI CAATTG 1 cut(s) 2433
MflI RGATCY 5 cut(s) 334, 457, 2044, 2106, 2254
MhlI GDGCHC 4 cut(s) 159, 270, 318, 3571
MlsI TGGCCA 1 cut(s) 3498
MluNI TGGCCA 1 cut(s) 3498
MlyI GAGTC 8 cut(s) 1444, 2275, 3275, 3635, 3644, 3813, 3822, 3926
MmeI TCCRAC 5 cut(s) 562, 631, 949, 2721, 3398
Mox20I TGGCCA 1 cut(s) 3498
Mph1103I ATGCAT 3 cut(s) 1523, 3209, 3340
MroI TCCGGA 1 cut(s) 2356
MroNI GCCGGC 1 cut(s) 3237
MroXI GAANNNNTTC 3 cut(s) 2142, 2352, 3173
MscI TGGCCA 1 cut(s) 3498
MslI CAYNNNNRTG 2 cut(s) 2090, 2300
Msp20I TGGCCA 1 cut(s) 3498
MspA1I CMGCKG 3 cut(s) 3589, 3957, 4013
MspCI CTTAAG 2 cut(s) 2066, 2276
MspI CCGG 6 cut(s) 552, 1653, 2357, 3001, 3238, 3478
MspR9I CCNGG 8 cut(s) 430, 552, 1401, 2561, 3056, 3099, 3479, 3746
MunI CAATTG 1 cut(s) 2433
Mva1269I GAATGC 5 cut(s) 290, 1484, 2085, 2295, 3761
MvaI CCWGG 6 cut(s) 430, 1401, 2561, 3056, 3099, 3746
MwoI GCNNNNNNNGC 4 cut(s) 1469, 2179, 2389, 2449
NaeI GCCGGC 1 cut(s) 3239
NciI CCSGG 2 cut(s) 552, 3479
NdeI CATATG 3 cut(s) 1030, 2091, 2301
NgoMIV GCCGGC 1 cut(s) 3237
NlaIV GGNNCC 6 cut(s) 315, 856, 2108, 2655, 3743, 3867
NmuCI GTSAC 7 cut(s) 593, 1841, 1904, 2726, 2821, 3448, 3805
NsiI ATGCAT 3 cut(s) 1523, 3209, 3340
NspI RCATGY 5 cut(s) 1085, 1332, 1834, 3091, 3207
NspV TTCGAA 1 cut(s) 349
PaeI GCATGC 1 cut(s) 1085
PaeR7I CTCGAG 1 cut(s) 158
PagI TCATGA 3 cut(s) 1074, 1566, 2373
PciI ACATGT 1 cut(s) 3087
PciSI GCTCTTC 2 cut(s) 147, 2028
PcsI WCGNNNNNNNCGW 2 cut(s) 198, 2110
PctI GAATGC 5 cut(s) 290, 1484, 2085, 2295, 3761
PdiI GCCGGC 1 cut(s) 3239
PdmI GAANNNNTTC 3 cut(s) 2142, 2352, 3173
PflFI GACNNNGTC 1 cut(s) 3803
PflMI CCANNNNNTGG 1 cut(s) 3505
PfoI TCCNGGA 1 cut(s) 3097
PleI GAGTC 8 cut(s) 1444, 2275, 3274, 3634, 3643, 3812, 3821, 3925
PpsI GAGTC 8 cut(s) 1444, 2275, 3274, 3634, 3643, 3812, 3821, 3925
PpuMI RGGWCCY 1 cut(s) 2557
PscI ACATGT 1 cut(s) 3087
PsiI TTATAA 3 cut(s) 747, 1053, 1194
Psp124BI GAGCTC 2 cut(s) 159, 270
Psp1406I AACGTT 2 cut(s) 760, 920
Psp5II RGGWCCY 1 cut(s) 2557
Psp6I CCWGG 6 cut(s) 428, 1399, 2559, 3054, 3097, 3744
PspFI CCCAGC 2 cut(s) 468, 3970
PspGI CCWGG 6 cut(s) 428, 1399, 2559, 3054, 3097, 3744
PspN4I GGNNCC 6 cut(s) 315, 856, 2108, 2655, 3743, 3867
PspPI GGNCC 4 cut(s) 554, 2557, 2670, 3944
PspPPI RGGWCCY 1 cut(s) 2557
PstNI CAGNNNCTG 1 cut(s) 1156
PsuI RGATCY 5 cut(s) 334, 457, 2044, 2106, 2254
PsyI GACNNNGTC 1 cut(s) 3803
PvuII CAGCTG 2 cut(s) 3589, 3957
RsaI GTAC 4 cut(s) 3009, 3245, 3364, 4002
RsaNI GTAC 4 cut(s) 3008, 3244, 3363, 4001
RseI CAYNNNNRTG 2 cut(s) 2090, 2300
SacI GAGCTC 2 cut(s) 159, 270
SapI GCTCTTC 2 cut(s) 147, 2028
Sau96I GGNCC 4 cut(s) 554, 2557, 2670, 3944
SchI GAGTC 8 cut(s) 1444, 2275, 3275, 3635, 3644, 3813, 3822, 3926
ScrFI CCNGG 8 cut(s) 430, 552, 1401, 2561, 3056, 3099, 3479, 3746
SduI GDGCHC 4 cut(s) 159, 270, 318, 3571
SfaNI GCATC 8 cut(s) 555, 1459, 1639, 1763, 2860, 2911, 3325, 3502
Sfr274I CTCGAG 1 cut(s) 158
SfuI TTCGAA 1 cut(s) 349
SinI GGWCC 2 cut(s) 554, 2557
SlaI CTCGAG 1 cut(s) 158
SmiMI CAYNNNNRTG 2 cut(s) 2090, 2300
SmlI CTYRAG 4 cut(s) 158, 1743, 2066, 2276
SmoI CTYRAG 4 cut(s) 158, 1743, 2066, 2276
SpeI ACTAGT 1 cut(s) 3392
SphI GCATGC 1 cut(s) 1085
SsiI CCGC 9 cut(s) 6, 790, 1247, 1778, 2409, 2470, 3402, 3515, 4013
SstI GAGCTC 2 cut(s) 159, 270
StyD4I CCNGG 8 cut(s) 428, 550, 1399, 2559, 3054, 3097, 3477, 3744
TaaI ACNGT 8 cut(s) 483, 1379, 1910, 2504, 2950, 3383, 3573, 3854
TaiI ACGT 4 cut(s) 753, 763, 923, 2568
TatI WGTACW 1 cut(s) 3243
TauI GCSGC 1 cut(s) 8
TscAI CASTG 7 cut(s) 836, 1217, 2242, 2685, 3171, 3229, 3859
TseFI GTSAC 7 cut(s) 593, 1841, 1904, 2726, 2821, 3448, 3805
Tsp45I GTSAC 7 cut(s) 593, 1841, 1904, 2726, 2821, 3448, 3805
TspGWI ACGGA 3 cut(s) 470, 1481, 2119
TspRI CASTG 7 cut(s) 836, 1217, 2242, 2685, 3171, 3229, 3859
Tth111I GACNNNGTC 1 cut(s) 3803
Van91I CCANNNNNTGG 1 cut(s) 3505
Vha464I CTTAAG 2 cut(s) 2066, 2276
VpaK11BI GGWCC 2 cut(s) 554, 2557
XagI CCTNNNNNAGG 1 cut(s) 410
XapI RAATTY 7 cut(s) 174, 891, 1658, 1951, 3016, 3039, 3871
XbaI TCTAGA 3 cut(s) 1300, 1318, 3262
XceI RCATGY 5 cut(s) 1085, 1332, 1834, 3091, 3207
XcmI CCANNNNNNNNNTGG 1 cut(s) 865
XhoI CTCGAG 1 cut(s) 158
XmiI GTMKAC 2 cut(s) 837, 1204
XmnI GAANNNNTTC 3 cut(s) 2142, 2352, 3173
Zsp2I ATGCAT 3 cut(s) 1523, 3209, 3340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.