Rw1G035830

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000067
Physical Location & Seq
Forward (+)
63608264 .. 63612708
4445 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G035830.1

Sequence Viewer

Length: 3906 bp
ATGGCGGCTTCATCTTCATCTGGTGCTCGTTGCAAGTATGATGTGTTCCTCAGTTTTAGAGGGGCAGACACCCGCAAGATCTTCGTCGGCCATCTTTACAGAGCTCTGAAACAGAAAACAATCAACACCTTCATCGATTCTGAAGAGCTCAGTAAAGGAAACAAGATTTCGGAGCTACTCACAGCTATTGAGGACTCGAGGATTTCGATTGTAGTTTTATCTCAAAAGTATGCATCTTCCACTTGGTGCTTAAAAGAGCTCGTCAAAATCCTGGATTGCATGGATACGAAGAAGCAGATAGTGGTGCCTATCTTCTACGAAGTAGATCCCTCTCATCTACGGAAAATCAATGGAAAATTTGCTGAAGCTTTTGCCAAACATGGAGAGAATTCTAACCTCGACAACGAAGAGTTGGAGAGCTGGAGGTCCGCTGTAAATAGACTCGCGGATCTATCTGGCTGGGATTCGACCAAATATGAGGATGACGGCAAGCTTGTTGAGGAAATTGTTGAAAATACTTTAAAAAAACTGATCCACACTTCGTCCAATAAAGCTGATGGCTTGGTTGGAATGGATTCCCACATTACAGAAATGAATTCAAGATTATGTCTTCAGGTGAATGATGTTCGTGTTGTTGGAATATGGGGTATGGGCGGTTTAGGAAAAACAACCATTGCTAGAGCTGTTTTTGACGAAATTGCTTGCCAATTTGAACACTCCTGCTTTCTTGATAATGTCAAAGAAGCTTTTATAAACAAGCGTGAAGTACAATTGCAGGAAGCACTTATATCTATATTGCTGAAGGAAAAGGTGCAGAGTCTGGGCTTGAACAGAGGTTGGAATATGATAAAGGAAAGGCTAGGTTTGAAAAAAGTCTTGGTTGTTCTTGATGATGTGGAAACTTTTGCCCAAATAGAAGCCTTGCTTGGAAAACTACATGCATTTGGTGAGGGAAGTAGGCTCATTGTAACAACTAGAAATAAGCAATCGCTAAGTGGAGTTAATGAGACGTTCAAGCCATCGTATTTAAGGGATGATGAAGCTCTCGAGCTGTTTCGGAAATATGCTTTCAGAACAAATCAACCCAACAGAGACTACGATGATCTATCAAGGCGTGCCATAGAATATGCTCAAGGTTTGCCTTTAGCACTTAAAGTCTTGGGGGCATTTCTTGATAACAGAAGTGTGTGTGAATGGGAAGTCGTGTTGGAGAAATTGAAGAAAATCCCACACATAGAAATTCAGGATGTGCTTAGAACAAGCTTTGATGGACTAGACGATTCAGAGAAAAACATATTCCTAGATATTGCATGTGTCTTTAGAAAATGGCGCACAAATTTTGTAACGGAATTACTGGACAGTTTTGGCTTCTTTCCCCACAATGGATTAAGAGTTCTAGTTGACAGAGCTCTCATAACTTCGTCAAATTACAGAGTCGACATGCATGATTTATTTCAAGAAATGGGTAGAGAAATCGTCCGCCTAGAATCTACTAAAAAACCTGGGAAGCGTAGCAGGTTGTGGAGTTATAAAGATGTTAATCACGTGTTGACTCAAAATACGGCTACGGATGCAATTGAATGCTTAATGCTAGACTTGTCGAACTCAAAAGTTGACCTAAGCATAAATGCTGAGGCCTTTGTAAGGATGACGAAGCTAAGACTGCTAATAATCTGTTATTCATTCCACGTATACCTTATTCCATATAGCGAGTGCCGCAAGAATATATGCCCAGTAGGTGGCTGTAAACAACTCTTGACGGGGGACTTCGAGTTTCTTTCCCATGAATTGAGGTTTCTCTTATGGCATGGATGCCCGGTAAAGTGTTTGCCATCATCTTTTATCCCGAAGAATCTTGTTCACCTTGACATGCGTGGTAGTCACATTCGAGAACTTTGGGAAGGAATCAAGCCCCTACAAAATCTGAGAGTCATCAAGTTAAGTCACTGTCGATACCTTGTCAAAATCCCCGACTTAACTGAGGCGATAAATCTTGAGACACTGCTTCTTGATGGCTGTTCAAGTTTATTTGAGGTTCATTCATCCATTTCTGCTCTTCAAAACCTTCATTCATTGGATCTTACCGGGTGCAAAAAACTCAAGATTCTCCCAAAGAGAATTCATATGAAATCTCTACAGACCCTTTGTCTTTCGGGCTGCTCAAATCTTGAAAAGTTTCCGGAGATTTCAGGAATCATGAAGGAGCTATCAATGCTTCGTTTAGATGGGACCGCAATTAAAGGACTGCCCTCATCAATCGATCTGCTTCTGAGGATTAAAAAATTAAGTGTGCGGAACTGCAAAAGCCTTGAATTTCTTCCCGACAGTATCTGTAATTTGGCAAACCTTACACATCTGAATCTGTCTGGGTGCTCGGTACTTCATTACTTGCCTGAGAAATTGGGGGACTTAAAATCCTTAGAGGTCCTCGATGTAGGATATAGTGGTATAAAACAACTTCCATTCTCTGTCCTACGTTTGGGAAAGCTTAAAGGTGGATTATCATGTATTGGATGTAAAAAGATGACGGCACCCTTGTCAGCCTGGCCATCATCAATGGAAGAATATTGTAGTTACTCTGTTCTGCTACGTCTTGATTTAAGTGACTGCAATCTGTTGCAGTTATCGGATGGTATTGCTCATTTATATTCATTGAAAACATTAAAGCTGTGCAGAAACAATTTGGAGAGCTTACCTGTGACAATGAATCGACTTCGTTGTCTAACGCATCTTGAATTGGAAGCTTGTAAGCGGCTGAAGTCAGTACCTGAGCTTTCACCGAGTGTAATACGCATAGATGCACATGATTGCACAGCTCTGGAAACAGTTTCAAGACGAAAGACTAATCCAGTCTGTAGAATTCTAGACTTCAGGTTTTCTAATTGCTTCCGGCTTCAAGCAAATTTGTTTATAGATATTGTTGAGACTGAGACTTATTCTCTCGACCAGGACAGTCATCTGCGACCTCTGTGGTTCCACATGTGGCTTCCTGGAAGAGAAGTTCCAGAATGGTTCAACCTTCGGGATACAGGATGTTCAGCAACTTTCCAAGCACCACTGAATAATTTCAATAACAAGCTTTTAGGATTTGCTATATGCATTGTTATTAAATTCAATGGTGCTCGTCATGATGTATCTGATATGTATGGAAGATGTTATTGGACCTCAAAAGGTAAGCATGGTGAGTACTTCTTCGAATTTGATTTGCTCGGCCGGCAGTTCGGAATTTACAGTATAGAGTCAGATCACCTGTTCATGGCATATTTGCCATGGTCTGGATATGGCAGGAATTCACAAGATAGATTAGATGCATTTAAGATTGTAGTAGAAACTGGAACTTATTCTCCGACAGAAGATTGGTTTTCTACTGGCTGCGGAGTTCGTTTAGTTTATGCTAATCAAGATGAGATGCAAAATAGCGTGACACAGCCCATAATTCATGTGGATGGCCAGACAATGGAGGATGCGGTGCAGCAGGGGGAGGGGTGCTGTGCTATAGGAGGGGTGTCGGGGCTTGGCGCACCTTTCTTTACGGACCAGTTGGAAATAAGAAAGAGTGAAACAGAGTTGGAGGCAGAGTCATCAGAGTCGCGCGAGGGGAGAGGCACGTGCCAGATATTGGAGCTGTTAACAGAGCCGCAGACTGAGAGAAAAGAAGAGGAGGATGGGGAGGGGTGTCAGGTGTTAGGGTCGGCATTCTCTTTGAGAAAGAGAGAAACAGAAAGAGAGGGAGACAGAGTCACAGGAGTCTCTGACGAGGGAGAGATATCGGAGTTGGGCAGCCACAGTGGTCTGGTGGTGCCAAATTCACAAAGTGAGGGCAAAGAAGAGAGAGAATTGATGGGGGAGGAGTCGTTGGAGAGGGTTGTTAGGGCCGCTGTGCAGCTGCTTTTACTGGCCGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1301

Amino Acids

146.97

Weight (kDa)

6.2

Isoelectric Point (pI)

40.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 12 - 190 1.9e-54 TIR domain
TIR_2 PF13676 15 - 116 1.4e-15 TIR domain
NB-ARC PF00931 195 - 360 3.1e-20 NB-ARC domain
WHD_ROQ1 PF23282 428 - 496 1.7e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 678 - 788 6.5e-07 Leucine-rich repeat region
LRR_13 PF23286 709 - 794 7.5e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 773 - 831 3.7e-09 Leucine-rich repeat region
LRR_8 PF13855 864 - 916 2.9e-06 Leucine rich repeat
C-JID PF20160 996 - 1135 7.1e-13 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 752, 1530
AasI GACNNNNNNGTC 1 cut(s) 2718
Acc36I ACCTGC 1 cut(s) 1506
AccB1I GGYRCC 3 cut(s) 304, 2530, 3799
AccB7I CCANNNNNTGG 4 cut(s) 1739, 3275, 3457, 3619
AccI GTMKAC 2 cut(s) 1437, 1692
AccII CGCG 3 cut(s) 446, 3592, 3594
AccIII TCCGGA 1 cut(s) 2179
AclWI GGATC 4 cut(s) 320, 456, 526, 2085
AcoI YGGCCR 5 cut(s) 88, 2546, 3211, 3448, 3897
AcuI CTGAAG 6 cut(s) 162, 384, 596, 821, 2777, 2854
AcvI CACGTG 2 cut(s) 1546, 3609
AdeI CACNNNGTG 3 cut(s) 246, 2783, 3815
AfaI GTAC 4 cut(s) 768, 2379, 2766, 3188
AfiI CCNNNNNNNGG 8 cut(s) 1382, 1644, 1739, 2479, 3256, 3275, 3457, 3619
AflIII ACRYGT 2 cut(s) 1545, 2979
AhdI GACNNNNNGTC 1 cut(s) 2145
AjnI CCWGG 5 cut(s) 270, 1501, 2543, 2946, 2989
AjuI GAANNNNNNNTTGG 6 cut(s) 860, 892, 909, 941, 3142, 3174
AloI GAACNNNNNNTCC 6 cut(s) 1377, 1409, 2985, 3017, 3328, 3360
Alw21I GWGCWC 7 cut(s) 28, 106, 150, 261, 1411, 2375, 3124
Alw26I GTCTC 7 cut(s) 1001, 1086, 1991, 2918, 2924, 3726, 3754
AlwI GGATC 4 cut(s) 320, 456, 526, 2085
AlwNI CAGNNNCTG 3 cut(s) 820, 2331, 2768
Ama87I CYCGRG 2 cut(s) 196, 1046
Aor13HI TCCGGA 1 cut(s) 2179
AoxI GGCC 7 cut(s) 88, 1635, 2546, 3211, 3448, 3873, 3897
ApeKI GCWGC 6 cut(s) 2157, 3372, 3472, 3780, 3883, 3886
Asp700I GAANNNNTTC 5 cut(s) 574, 2175, 2316, 3065, 3340
AspLEI GCGC 3 cut(s) 1332, 3521, 3594
AspS9I GGNCC 6 cut(s) 426, 2229, 2425, 3162, 3535, 3873
AsuC2I CCSGG 3 cut(s) 1817, 2086, 3901
AsuHPI GGTGA 6 cut(s) 628, 959, 1853, 2769, 3194, 3239
AsuII TTCGAA 1 cut(s) 3195
AvaI CYCGRG 2 cut(s) 196, 1046
AvaII GGWCC 5 cut(s) 426, 2229, 2425, 3162, 3535
BalI TGGCCA 2 cut(s) 2548, 3450
BanI GGYRCC 3 cut(s) 304, 2530, 3799
BanII GRGCYC 4 cut(s) 106, 150, 261, 1411
BbrPI CACGTG 2 cut(s) 1546, 3609
BbsI GAAGAC 1 cut(s) 602
Bbv12I GWGCWC 7 cut(s) 28, 106, 150, 261, 1411, 2375, 3124
BbvCI CCTCAGC 1 cut(s) 1632
BbvI GCAGC 6 cut(s) 2144, 3359, 3484, 3792, 3873, 3895
BceAI ACGGC 3 cut(s) 502, 1578, 2544
BcgI CGANNNNNNTGC 2 cut(s) 64, 98
BciT130I CCWGG 5 cut(s) 272, 1503, 2545, 2948, 2991
BciVI GTATCC 2 cut(s) 277, 3019
BcnI CCSGG 3 cut(s) 1817, 2086, 3901
BcoDI GTCTC 7 cut(s) 1001, 1086, 1991, 2918, 2924, 3726, 3754
BfaI CTAG 9 cut(s) 678, 860, 975, 1274, 1301, 1397, 1484, 1592, 2864
BfmI CTRYAG 3 cut(s) 2135, 2854, 3495
BfuAI ACCTGC 1 cut(s) 1506
BfuI GTATCC 2 cut(s) 277, 3019
BglII AGATCT 1 cut(s) 78
BmcAI AGTACT 1 cut(s) 3188
Bme1390I CCNGG 8 cut(s) 272, 1503, 1817, 2086, 2545, 2948, 2991, 3901
Bme18I GGWCC 5 cut(s) 426, 2229, 2425, 3162, 3535
BmeRI GACNNNNNGTC 1 cut(s) 2145
BmeT110I CYCGRG 2 cut(s) 196, 1046
BmgT120I GGNCC 6 cut(s) 426, 2229, 2425, 3162, 3535, 3873
BmiI GGNNCC 5 cut(s) 306, 2230, 2532, 2975, 3801
BmrFI CCNGG 8 cut(s) 272, 1503, 1817, 2086, 2545, 2948, 2991, 3901
BmrI ACTGGG 1 cut(s) 1727
BmsI GCATC 8 cut(s) 242, 1561, 1802, 2737, 2788, 3298, 3399, 3454
BmuI ACTGGG 1 cut(s) 1727
BpiI GAAGAC 1 cut(s) 602
BpmI CTGGAG 1 cut(s) 442
Bpu10I CCTNAGC 3 cut(s) 1619, 1632, 2769
Bpu14I TTCGAA 1 cut(s) 3195
BpuEI CTTGAG 3 cut(s) 1116, 2015, 2084
BpuMI CCSGG 3 cut(s) 1817, 2086, 3901
Bsa29I ATCGAT 2 cut(s) 135, 2259
BsaAI YACGTR 3 cut(s) 1546, 1690, 3609
BsaBI GATNNNNATC 1 cut(s) 1539
BsaJI CCNNGG 2 cut(s) 1502, 3269
BsaWI WCCGGW 1 cut(s) 2179
BsaXI ACNNNNNCTCC 8 cut(s) 3328, 3358, 3563, 3593, 3723, 3753, 3839, 3869
Bsc4I CCNNNNNNNGG 8 cut(s) 1382, 1644, 1739, 2479, 3256, 3275, 3457, 3619
Bse118I RCCGGY 1 cut(s) 3213
Bse1I ACTGG 7 cut(s) 1359, 1733, 2849, 3337, 3373, 3538, 3900
Bse3DI GCAATG 1 cut(s) 672
Bse8I GATNNNNATC 1 cut(s) 1539
BseAI TCCGGA 1 cut(s) 2179
BseBI CCWGG 5 cut(s) 272, 1503, 2545, 2948, 2991
BseCI ATCGAT 2 cut(s) 135, 2259
BseDI CCNNGG 2 cut(s) 1502, 3269
BseJI GATNNNNATC 1 cut(s) 1539
BseLI CCNNNNNNNGG 8 cut(s) 1382, 1644, 1739, 2479, 3256, 3275, 3457, 3619
BseMI GCAATG 1 cut(s) 672
BseNI ACTGG 7 cut(s) 1359, 1733, 2849, 3337, 3373, 3538, 3900
BseRI GAGGAG 2 cut(s) 3674, 3863
BseX3I CGGCCG 1 cut(s) 3211
BseXI GCAGC 6 cut(s) 2144, 3359, 3484, 3792, 3873, 3895
BseYI CCCAGC 1 cut(s) 459
BsgI GTGCAG 4 cut(s) 833, 2692, 3491, 3902
Bsh1236I CGCG 3 cut(s) 446, 3592, 3594
Bsh1285I CGRYCG 1 cut(s) 3214
BshFI GGCC 7 cut(s) 90, 1637, 2548, 3213, 3450, 3875, 3899
BshNI GGYRCC 3 cut(s) 304, 2530, 3799
BshVI ATCGAT 2 cut(s) 135, 2259
BsiEI CGRYCG 1 cut(s) 3214
BsiHKAI GWGCWC 7 cut(s) 28, 106, 150, 261, 1411, 2375, 3124
BsiHKCI CYCGRG 2 cut(s) 196, 1046
BsiSI CCGG 6 cut(s) 1817, 2085, 2180, 2890, 3214, 3900
BslFI GGGAC 3 cut(s) 1778, 2242, 2420
BslI CCNNNNNNNGG 8 cut(s) 1382, 1644, 1739, 2479, 3256, 3275, 3457, 3619
BsmAI GTCTC 7 cut(s) 1001, 1086, 1991, 2918, 2924, 3726, 3754
BsmBI CGTCTC 1 cut(s) 1001
BsmFI GGGAC 3 cut(s) 1778, 2242, 2420
BsmI GAATGC 2 cut(s) 1586, 3695
BsnI GGCC 7 cut(s) 90, 1637, 2548, 3213, 3450, 3875, 3899
BsoBI CYCGRG 2 cut(s) 196, 1046
Bsp119I TTCGAA 1 cut(s) 3195
Bsp1286I GDGCHC 7 cut(s) 28, 106, 150, 261, 1411, 2375, 3124
Bsp13I TCCGGA 1 cut(s) 2179
Bsp143I GATC 8 cut(s) 78, 325, 448, 531, 1102, 2077, 2260, 3244
Bsp19I CCATGG 1 cut(s) 3269
BspANI GGCC 7 cut(s) 90, 1637, 2548, 3213, 3450, 3875, 3899
BspDI ATCGAT 2 cut(s) 135, 2259
BspEI TCCGGA 1 cut(s) 2179
BspFNI CGCG 3 cut(s) 446, 3592, 3594
BspHI TCATGA 2 cut(s) 2196, 3127
BspLI GGNNCC 5 cut(s) 306, 2230, 2532, 2975, 3801
BspMI ACCTGC 1 cut(s) 1506
BspPI GGATC 4 cut(s) 320, 456, 526, 2085
BspQI GCTCTTC 2 cut(s) 138, 2061
BspT104I TTCGAA 1 cut(s) 3195
BspT107I GGYRCC 3 cut(s) 304, 2530, 3799
BsrDI GCAATG 1 cut(s) 672
BsrFI RCCGGY 1 cut(s) 3213
BsrI ACTGG 7 cut(s) 1359, 1733, 2849, 3337, 3373, 3538, 3900
BssAI RCCGGY 1 cut(s) 3213
BssECI CCNNGG 2 cut(s) 1502, 3269
BssMI GATC 8 cut(s) 78, 325, 448, 531, 1102, 2077, 2260, 3244
BssNAI GTATAC 1 cut(s) 1693
BssT1I CCWWGG 1 cut(s) 3269
Bst1107I GTATAC 1 cut(s) 1693
Bst2UI CCWGG 5 cut(s) 272, 1503, 2545, 2948, 2991
Bst4CI ACNGT 7 cut(s) 1361, 1949, 2327, 2827, 2954, 3233, 3788
Bst6I CTCTTC 6 cut(s) 138, 402, 2061, 2989, 3651, 3822
BstBAI YACGTR 3 cut(s) 1546, 1690, 3609
BstBI TTCGAA 1 cut(s) 3195
BstC8I GCNNGC 4 cut(s) 491, 703, 1116, 3215
BstDSI CCRYGG 1 cut(s) 3269
BstENI CCTNNNNNAGG 1 cut(s) 1642
BstFNI CGCG 3 cut(s) 446, 3592, 3594
BstHHI GCGC 3 cut(s) 1332, 3521, 3594
BstKTI GATC 8 cut(s) 81, 328, 451, 534, 1105, 2080, 2263, 3247
BstMAI GTCTC 7 cut(s) 1001, 1086, 1991, 2918, 2924, 3726, 3754
BstMBI GATC 8 cut(s) 78, 325, 448, 531, 1102, 2077, 2260, 3244
BstMCI CGRYCG 1 cut(s) 3214
BstMWI GCNNNNNNNGC 5 cut(s) 1571, 1663, 1716, 2212, 3214
BstNI CCWGG 5 cut(s) 272, 1503, 2545, 2948, 2991
BstNSI RCATGY 5 cut(s) 941, 1314, 1444, 1873, 2983
BstSCI CCNGG 8 cut(s) 270, 1501, 1815, 2084, 2543, 2946, 2989, 3899
BstSFI CTRYAG 3 cut(s) 2135, 2854, 3495
BstUI CGCG 3 cut(s) 446, 3592, 3594
BstV1I GCAGC 6 cut(s) 2144, 3359, 3484, 3792, 3873, 3895
BstV2I GAAGAC 1 cut(s) 602
BstX2I RGATCY 4 cut(s) 78, 325, 448, 2077
BstYI RGATCY 4 cut(s) 78, 325, 448, 2077
BstZ17I GTATAC 1 cut(s) 1693
BstZI CGGCCG 1 cut(s) 3211
Bsu15I ATCGAT 2 cut(s) 135, 2259
BsuI GTATCC 2 cut(s) 277, 3019
BsuRI GGCC 7 cut(s) 90, 1637, 2548, 3213, 3450, 3875, 3899
BsuTUI ATCGAT 2 cut(s) 135, 2259
BtgI CCRYGG 1 cut(s) 3269
BtsI GCAGTG 1 cut(s) 2000
BtsIMutI CAGTG 4 cut(s) 1945, 2000, 3056, 3793
BveI ACCTGC 1 cut(s) 1506
Cac8I GCNNGC 4 cut(s) 491, 703, 1116, 3215
CaiI CAGNNNCTG 3 cut(s) 820, 2331, 2768
CciI TCATGA 2 cut(s) 2196, 3127
CfoI GCGC 3 cut(s) 1332, 3521, 3594
Cfr10I RCCGGY 1 cut(s) 3213
Cfr13I GGNCC 6 cut(s) 426, 2229, 2425, 3162, 3535, 3873
ClaI ATCGAT 2 cut(s) 135, 2259
Csp6I GTAC 4 cut(s) 767, 2378, 2765, 3187
CviQI GTAC 4 cut(s) 767, 2378, 2765, 3187
DpnI GATC 8 cut(s) 80, 327, 450, 533, 1104, 2079, 2262, 3246
DpnII GATC 8 cut(s) 78, 325, 448, 531, 1102, 2077, 2260, 3244
DraI TTTAAA 1 cut(s) 522
DraIII CACNNNGTG 3 cut(s) 246, 2783, 3815
DrdI GACNNNNNNGTC 1 cut(s) 2718
DriI GACNNNNNGTC 1 cut(s) 2145
DseDI GACNNNNNNGTC 1 cut(s) 2718
EaeI YGGCCR 5 cut(s) 88, 2546, 3211, 3448, 3897
EagI CGGCCG 1 cut(s) 3211
Eam1104I CTCTTC 6 cut(s) 138, 402, 2061, 2989, 3651, 3822
Eam1105I GACNNNNNGTC 1 cut(s) 2145
EarI CTCTTC 6 cut(s) 138, 402, 2061, 2989, 3651, 3822
EciI GGCGGA 1 cut(s) 1469
Ecl136II GAGCTC 4 cut(s) 104, 148, 259, 1409
EclXI CGGCCG 1 cut(s) 3211
Eco130I CCWWGG 1 cut(s) 3269
Eco147I AGGCCT 1 cut(s) 1637
Eco24I GRGCYC 4 cut(s) 106, 150, 261, 1411
Eco32I GATATC 1 cut(s) 3768
Eco47I GGWCC 5 cut(s) 426, 2229, 2425, 3162, 3535
Eco52I CGGCCG 1 cut(s) 3211
Eco53kI GAGCTC 4 cut(s) 104, 148, 259, 1409
Eco57I CTGAAG 6 cut(s) 162, 384, 596, 821, 2777, 2854
Eco72I CACGTG 2 cut(s) 1546, 3609
Eco88I CYCGRG 2 cut(s) 196, 1046
EcoICRI GAGCTC 4 cut(s) 104, 148, 259, 1409
EcoNI CCTNNNNNAGG 1 cut(s) 1642
EcoO109I RGGNCCY 1 cut(s) 2425
EcoRI GAATTC 5 cut(s) 388, 595, 2118, 2859, 3289
EcoRII CCWGG 5 cut(s) 270, 1501, 2543, 2946, 2989
EcoRV GATATC 1 cut(s) 3768
EcoT14I CCWWGG 1 cut(s) 3269
EcoT22I ATGCAT 5 cut(s) 235, 943, 1446, 3101, 3313
EcoT38I GRGCYC 4 cut(s) 106, 150, 261, 1411
ErhI CCWWGG 1 cut(s) 3269
Esp3I CGTCTC 1 cut(s) 1001
FalI AAGNNNNNCTT 2 cut(s) 909, 941
FaqI GGGAC 3 cut(s) 1778, 2242, 2420
FauI CCCGC 1 cut(s) 80
FauNDI CATATG 1 cut(s) 2124
FblI GTMKAC 2 cut(s) 1437, 1692
FriOI GRGCYC 4 cut(s) 106, 150, 261, 1411
FspBI CTAG 9 cut(s) 678, 860, 975, 1274, 1301, 1397, 1484, 1592, 2864
GlaI GCGC 3 cut(s) 1331, 3520, 3593
GsaI CCCAGC 1 cut(s) 463
GsuI CTGGAG 1 cut(s) 442
HaeIII GGCC 7 cut(s) 90, 1637, 2548, 3213, 3450, 3875, 3899
HapII CCGG 6 cut(s) 1817, 2085, 2180, 2890, 3214, 3900
HhaI GCGC 3 cut(s) 1332, 3521, 3594
Hin6I GCGC 3 cut(s) 1330, 3519, 3592
HinP1I GCGC 3 cut(s) 1330, 3519, 3592
HincII GTYRAC 5 cut(s) 1402, 1438, 1551, 1615, 3630
HindII GTYRAC 5 cut(s) 1402, 1438, 1551, 1615, 3630
HindIII AAGCTT 7 cut(s) 366, 491, 744, 1261, 2486, 2742, 3077
HpaI GTTAAC 1 cut(s) 3630
HpaII CCGG 6 cut(s) 1817, 2085, 2180, 2890, 3214, 3900
HphI GGTGA 6 cut(s) 628, 959, 1853, 2769, 3194, 3239
Hpy166II GTNNAC 8 cut(s) 1402, 1438, 1551, 1615, 1693, 1748, 1861, 3630
Hpy8I GTNNAC 8 cut(s) 1402, 1438, 1551, 1615, 1693, 1748, 1861, 3630
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 6 cut(s) 139, 796, 1895, 2075, 2194, 3029
HpyCH4III ACNGT 7 cut(s) 1361, 1949, 2327, 2827, 2954, 3233, 3788
HpyCH4IV ACGT 6 cut(s) 1010, 1545, 1689, 2476, 2590, 3608
HpyF10VI GCNNNNNNNGC 5 cut(s) 1571, 1663, 1716, 2212, 3214
HpySE526I ACGT 6 cut(s) 1010, 1545, 1689, 2476, 2590, 3608
HspAI GCGC 3 cut(s) 1330, 3519, 3592
Kpn2I TCCGGA 1 cut(s) 2179
KroI GCCGGC 1 cut(s) 3213
KroNI GCCGGC 1 cut(s) 3215
KspAI GTTAAC 1 cut(s) 3630
Kzo9I GATC 8 cut(s) 78, 325, 448, 531, 1102, 2077, 2260, 3244
LguI GCTCTTC 2 cut(s) 138, 2061
LmnI GCTCC 3 cut(s) 172, 2203, 3622
Lsp1109I GCAGC 6 cut(s) 2144, 3359, 3484, 3792, 3873, 3895
LweI GCATC 8 cut(s) 242, 1561, 1802, 2737, 2788, 3298, 3399, 3454
MaeI CTAG 9 cut(s) 678, 860, 975, 1274, 1301, 1397, 1484, 1592, 2864
MaeII ACGT 6 cut(s) 1010, 1545, 1689, 2476, 2590, 3608
MaeIII GTNAC 9 cut(s) 967, 1342, 1880, 1943, 2573, 2603, 2698, 3421, 3739
MalI GATC 8 cut(s) 80, 327, 450, 533, 1104, 2079, 2262, 3246
MboI GATC 8 cut(s) 78, 325, 448, 531, 1102, 2077, 2260, 3244
MfeI CAATTG 2 cut(s) 770, 1575
MflI RGATCY 4 cut(s) 78, 325, 448, 2077
MhlI GDGCHC 7 cut(s) 28, 106, 150, 261, 1411, 2375, 3124
MlsI TGGCCA 2 cut(s) 2548, 3450
MluNI TGGCCA 2 cut(s) 2548, 3450
MmeI TCCRAC 9 cut(s) 393, 547, 616, 818, 1188, 3371, 3522, 3549, 3837
Mox20I TGGCCA 2 cut(s) 2548, 3450
Mph1103I ATGCAT 5 cut(s) 235, 943, 1446, 3101, 3313
MroI TCCGGA 1 cut(s) 2179
MroNI GCCGGC 1 cut(s) 3213
MroXI GAANNNNTTC 5 cut(s) 574, 2175, 2316, 3065, 3340
MscI TGGCCA 2 cut(s) 2548, 3450
MslI CAYNNNNRTG 1 cut(s) 3444
Msp20I TGGCCA 2 cut(s) 2548, 3450
MspA1I CMGCKG 3 cut(s) 431, 3878, 3886
MspI CCGG 6 cut(s) 1817, 2085, 2180, 2890, 3214, 3900
MspR9I CCNGG 8 cut(s) 272, 1503, 1817, 2086, 2545, 2948, 2991, 3901
MunI CAATTG 2 cut(s) 770, 1575
Mva1269I GAATGC 2 cut(s) 1586, 3695
MvaI CCWGG 5 cut(s) 272, 1503, 2545, 2948, 2991
MvnI CGCG 3 cut(s) 446, 3592, 3594
MwoI GCNNNNNNNGC 5 cut(s) 1571, 1663, 1716, 2212, 3214
NaeI GCCGGC 1 cut(s) 3215
NciI CCSGG 3 cut(s) 1817, 2086, 3901
NcoI CCATGG 1 cut(s) 3269
NdeI CATATG 1 cut(s) 2124
NdeII GATC 8 cut(s) 78, 325, 448, 531, 1102, 2077, 2260, 3244
NgoMIV GCCGGC 1 cut(s) 3213
NlaIV GGNNCC 5 cut(s) 306, 2230, 2532, 2975, 3801
NmeAIII GCCGAG 1 cut(s) 3189
NmuCI GTSAC 6 cut(s) 1880, 1943, 2603, 2698, 3421, 3739
NsiI ATGCAT 5 cut(s) 235, 943, 1446, 3101, 3313
NspI RCATGY 5 cut(s) 941, 1314, 1444, 1873, 2983
NspV TTCGAA 1 cut(s) 3195
PaeR7I CTCGAG 2 cut(s) 196, 1046
PagI TCATGA 2 cut(s) 2196, 3127
PceI AGGCCT 1 cut(s) 1637
PciI ACATGT 1 cut(s) 2979
PciSI GCTCTTC 2 cut(s) 138, 2061
PcsI WCGNNNNNNNCGW 1 cut(s) 203
PctI GAATGC 2 cut(s) 1586, 3695
PdiI GCCGGC 1 cut(s) 3215
PdmI GAANNNNTTC 5 cut(s) 574, 2175, 2316, 3065, 3340
PflFI GACNNNGTC 1 cut(s) 3737
PflMI CCANNNNNTGG 4 cut(s) 1739, 3275, 3457, 3619
PfoI TCCNGGA 2 cut(s) 270, 2989
PmaCI CACGTG 2 cut(s) 1546, 3609
PmlI CACGTG 2 cut(s) 1546, 3609
Ppu21I YACGTR 3 cut(s) 1546, 1690, 3609
PpuMI RGGWCCY 1 cut(s) 2425
PscI ACATGT 1 cut(s) 2979
PsiI TTATAA 2 cut(s) 752, 1530
Psp124BI GAGCTC 4 cut(s) 106, 150, 261, 1411
Psp5II RGGWCCY 1 cut(s) 2425
Psp6I CCWGG 5 cut(s) 270, 1501, 2543, 2946, 2989
PspCI CACGTG 2 cut(s) 1546, 3609
PspFI CCCAGC 1 cut(s) 459
PspGI CCWGG 5 cut(s) 270, 1501, 2543, 2946, 2989
PspN4I GGNNCC 5 cut(s) 306, 2230, 2532, 2975, 3801
PspPI GGNCC 6 cut(s) 426, 2229, 2425, 3162, 3535, 3873
PspPPI RGGWCCY 1 cut(s) 2425
PspXI VCTCGAGB 1 cut(s) 196
PsrI GAACNNNNNNTAC 2 cut(s) 29, 61
PstNI CAGNNNCTG 3 cut(s) 820, 2331, 2768
PsuI RGATCY 4 cut(s) 78, 325, 448, 2077
PsyI GACNNNGTC 1 cut(s) 3737
PvuII CAGCTG 1 cut(s) 3886
RsaI GTAC 4 cut(s) 768, 2379, 2766, 3188
RsaNI GTAC 4 cut(s) 767, 2378, 2765, 3187
RseI CAYNNNNRTG 1 cut(s) 3444
SacI GAGCTC 4 cut(s) 106, 150, 261, 1411
SalI GTCGAC 1 cut(s) 1436
SapI GCTCTTC 2 cut(s) 138, 2061
Sau3AI GATC 8 cut(s) 78, 325, 448, 531, 1102, 2077, 2260, 3244
Sau96I GGNCC 6 cut(s) 426, 2229, 2425, 3162, 3535, 3873
ScaI AGTACT 1 cut(s) 3188
ScrFI CCNGG 8 cut(s) 272, 1503, 1817, 2086, 2545, 2948, 2991, 3901
SduI GDGCHC 7 cut(s) 28, 106, 150, 261, 1411, 2375, 3124
SfaNI GCATC 8 cut(s) 242, 1561, 1802, 2737, 2788, 3298, 3399, 3454
SfcI CTRYAG 3 cut(s) 2135, 2854, 3495
Sfr274I CTCGAG 2 cut(s) 196, 1046
SfuI TTCGAA 1 cut(s) 3195
SinI GGWCC 5 cut(s) 426, 2229, 2425, 3162, 3535
SlaI CTCGAG 2 cut(s) 196, 1046
SmiMI CAYNNNNRTG 1 cut(s) 3444
SmlI CTYRAG 5 cut(s) 196, 1046, 1131, 1994, 2099
SmoI CTYRAG 5 cut(s) 196, 1046, 1131, 1994, 2099
SseBI AGGCCT 1 cut(s) 1637
SspI AATATT 1 cut(s) 2567
SspMI CTAG 9 cut(s) 678, 860, 975, 1274, 1301, 1397, 1484, 1592, 2864
SstI GAGCTC 4 cut(s) 106, 150, 261, 1411
StuI AGGCCT 1 cut(s) 1637
StyD4I CCNGG 8 cut(s) 270, 1501, 1815, 2084, 2543, 2946, 2989, 3899
StyI CCWWGG 1 cut(s) 3269
TaaI ACNGT 7 cut(s) 1361, 1949, 2327, 2827, 2954, 3233, 3788
TaiI ACGT 6 cut(s) 1013, 1548, 1692, 2479, 2593, 3611
TatI WGTACW 2 cut(s) 766, 3186
TauI GCSGC 5 cut(s) 8, 1719, 2755, 3640, 3878
TscAI CASTG 4 cut(s) 1952, 2007, 3063, 3793
TseFI GTSAC 6 cut(s) 1880, 1943, 2603, 2698, 3421, 3739
TseI GCWGC 6 cut(s) 2157, 3372, 3472, 3780, 3883, 3886
Tsp45I GTSAC 6 cut(s) 1880, 1943, 2603, 2698, 3421, 3739
TspGWI ACGGA 4 cut(s) 355, 1361, 1583, 3548
TspRI CASTG 4 cut(s) 1952, 2007, 3063, 3793
Tth111I GACNNNGTC 1 cut(s) 3737
Van91I CCANNNNNTGG 4 cut(s) 1739, 3275, 3457, 3619
VpaK11BI GGWCC 5 cut(s) 426, 2229, 2425, 3162, 3535
XagI CCTNNNNNAGG 1 cut(s) 1642
XbaI TCTAGA 1 cut(s) 2863
XceI RCATGY 5 cut(s) 941, 1314, 1444, 1873, 2983
XcmI CCANNNNNNNNNTGG 1 cut(s) 3439
XhoI CTCGAG 2 cut(s) 196, 1046
XmiI GTMKAC 2 cut(s) 1437, 1692
XmnI GAANNNNTTC 5 cut(s) 574, 2175, 2316, 3065, 3340
XspI CTAG 9 cut(s) 678, 860, 975, 1274, 1301, 1397, 1484, 1592, 2864
ZrmI AGTACT 1 cut(s) 3188
Zsp2I ATGCAT 5 cut(s) 235, 943, 1446, 3101, 3313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.