Rroxscaffold_4G00305840

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000067
Physical Location & Seq
Forward (+)
26531744 .. 26534816
3073 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00305840.1

Sequence Viewer

Length: 1818 bp
ATGGAAGCACGATCAAGGTCTACTTCGAACCCTTCATCGACTAGTTCGGCAACAAGAAGAGGGTCGACATCGTGGCCTGGTTCGGCTACGGAAGCTGTTGAAGGCATAATCCTAGACTTAACAAAGTTAAAAGAGGTATACGTAAATGATGAAGCCTTCGTTAGCATGAGGAGACTACGGCTACTCCAGCTTTCTGAATATAATTATGGAGATGGATATGTTTATGGAAGAGAATGGATGCGAGATGCAGATTATGATCCCTGTGTACAATACGTGAGTGAGAACTTAAAGTTTCTTTTGAATGATTTGAGAATTCTTGTGTGGCATGGATGCCCACTAATGTCTTTCCCAATTAATTTTCTACCGAAGAATCTTATTTACGTTGACATGTGTTATAGTCGTATTGAACAACTTTGGGAAGGAACCAAGCGTTTGCAAAACTTGATATACATAAATTTAAGTCGGTCAAAATATCTAATCAAAACCCCCGACTTCACTGGGGCAACAAATCTTGAGAAACTGAAGCTTAAAGAATGTACAAGTTTATTGGAAGTTCACCCATCCATTTCAGCTCTTAAAAGCCTCGTTGAGTTGGACCTACGAGGCTGCTTAAATCTGAAGAAGTTTCCAAATATATCCGGAAACATGAGGGAGCTAAGAAAACTTTATTTAGATGAGACTGCAATTACAGAAATGCCCCCGTCAATCAATGATCTTTCCAAGCTGCAGACTCTGAGCCTCGCTGGTTGCAGAGAACTCAGGAGTCTTTCGAGCAGCATTCACATGAAATCTCTGGAATCTCTTGAACTATCTGGTTGCTTGAAACTTACGGAGTTTCCAGAGATTTCAGAAGTAATGAAGAAGCTATGGAAGCTTGAATTAAAAGGGACAGCAATTAAAGAACTACCCTCATCTATTAATAATCTTACGGGGCTTGGGTATTTAAATTTGACAAACTGCACAAGCCTTGTCTGTCTTCCGGACAATATCTGCAAGTTGGAATGCCTTTTTCCACTCCGTCTGACTGGGTGCTCTAACCTTTCAAAGTTGCCTGAGAACTATGGGAATTTGAAATCCTTGTCACCCAAGTATGGCCTCTCCAGTGAACTGGAGGGAAGTGGTATAAAACAACTTCCATTCTCTATCTTGGGTTTGACTTCATTATCATGTAATGGATGTAAAGAGATGACGGCGCCCTTTATTTCGTGGCCAAAATGGAACCACCAAAATCATAGTTGCTCTGTTCTGTTCAAACTTGATTTAAGTGACTGCAATCTCTTGGAATTATCGGATGGTATTGCTTATTTATCTTCACTGCAAGTTTTAGACTTATCCAAAAACAACAAGTTGGAAAGTTTACCTGCAGCAATGAATCGCCTGGGTCATTTAACACGGCTCTATCTGAGAGGTTGCAAGAGACTGAAATCAATACCTGAGCTTTCATCGAGTATACAAGAAATCAATGCCAAAAATTGCACATCTTTGGAGACAGAGTATTATAAATGCCGTATGACTCTTCCTGGAAGTGATATTCCAGATTGGTTCAACCATCAGAGTGGAGGGTCATCCGTAACTGTGCCGCTACCACCCAATTGGCTCGCAAGCAACTTTCTCGGGTTCGCTATATGCGCCATCAGTAACTTGCGGGGTGCTGCTAATAGAAAAACTCTAGATTCTACCACCTGTTGCTGTACCTTAAAAGCAAATGACGATCAGGACAATTTCTATTTTCCTCTGCTCCCCCTTTTCGAGGTGGAACTGACGTGCCCCTTGATTTGGATCATATGTTGCTGGGATATACAAATTTCCGAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

605

Amino Acids

67.99

Weight (kDa)

7.33

Isoelectric Point (pI)

44.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 184 - 292 3.3e-08 Leucine-rich repeat region
LRR_13 PF23286 192 - 262 1.2e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 262 - 348 2.7e-13 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 268 - 361 3.7e-09 Leucine-rich repeat region
LRR_8 PF13855 418 - 471 4.5e-08 Leucine rich repeat
LRR_14 PF23598 419 - 492 1.2e-07 Leucine-rich repeat region
LRR_4 PF12799 438 - 481 5.9e-06 Leucine Rich repeats (2 copies)
C-JID PF20160 506 - 567 4.2e-17 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1500
Acc36I ACCTGC 1 cut(s) 1369
AccB1I GGYRCC 1 cut(s) 1192
AccI GTMKAC 4 cut(s) 20, 65, 138, 1450
AccIII TCCGGA 2 cut(s) 638, 979
AciI CCGC 2 cut(s) 1580, 1645
AclWI GGATC 2 cut(s) 251, 1787
AcoI YGGCCR 1 cut(s) 1208
AcsI RAATTY 5 cut(s) 312, 454, 946, 1066, 1803
AcuI CTGAAG 2 cut(s) 542, 638
AcyI GRCGYC 1 cut(s) 1193
AfaI GTAC 3 cut(s) 267, 538, 1693
AfiI CCNNNNNNNGG 3 cut(s) 1091, 1750, 1776
AflIII ACRYGT 1 cut(s) 387
AhlI ACTAGT 1 cut(s) 41
AjiI CACGTC 1 cut(s) 1764
AjnI CCWGG 3 cut(s) 76, 1377, 1519
AluBI AGCT 9 cut(s) 95, 190, 526, 572, 655, 724, 865, 874, 1438
AluI AGCT 9 cut(s) 95, 190, 526, 572, 655, 724, 865, 874, 1438
Alw21I GWGCWC 1 cut(s) 1034
Alw26I GTCTC 4 cut(s) 166, 671, 1411, 1481
AlwI GGATC 2 cut(s) 251, 1787
AlwNI CAGNNNCTG 1 cut(s) 733
Ama87I CYCGRG 1 cut(s) 1613
Aor13HI TCCGGA 2 cut(s) 638, 979
AoxI GGCC 3 cut(s) 74, 1093, 1208
ApeKI GCWGC 5 cut(s) 606, 724, 774, 1364, 1652
ApoI RAATTY 5 cut(s) 312, 454, 946, 1066, 1803
AseI ATTAAT 2 cut(s) 354, 918
AspLEI GCGC 2 cut(s) 1195, 1631
AspS9I GGNCC 1 cut(s) 595
AsuHPI GGTGA 2 cut(s) 548, 1074
AsuII TTCGAA 1 cut(s) 26
AvaI CYCGRG 1 cut(s) 1613
AvaII GGWCC 1 cut(s) 595
BaeGI GKGCMC 1 cut(s) 1769
BalI TGGCCA 1 cut(s) 1210
BanI GGYRCC 1 cut(s) 1192
BbsI GAAGAC 1 cut(s) 968
Bbv12I GWGCWC 1 cut(s) 1034
BbvI GCAGC 5 cut(s) 593, 711, 786, 1376, 1639
BccI CCATC 5 cut(s) 206, 568, 1286, 1557, 1640
BceAI ACGGC 4 cut(s) 194, 1206, 1409, 1491
BcgI CGANNNNNNTGC 2 cut(s) 1594, 1628
BciT130I CCWGG 3 cut(s) 78, 1379, 1521
BcoDI GTCTC 4 cut(s) 166, 671, 1411, 1481
BcuI ACTAGT 1 cut(s) 41
BfaI CTAG 3 cut(s) 42, 113, 1670
BfmI CTRYAG 2 cut(s) 725, 1362
BfoI RGCGCY 1 cut(s) 1196
BfuAI ACCTGC 1 cut(s) 1369
BisI GCNGC 6 cut(s) 607, 725, 775, 1365, 1580, 1653
BlsI GCNGC 6 cut(s) 608, 726, 776, 1366, 1581, 1654
Bme1390I CCNGG 3 cut(s) 78, 1379, 1521
Bme18I GGWCC 1 cut(s) 595
BmeT110I CYCGRG 1 cut(s) 1613
BmgBI CACGTC 1 cut(s) 1764
BmgT120I GGNCC 1 cut(s) 595
BmiI GGNNCC 3 cut(s) 424, 1194, 1220
BmrFI CCNGG 3 cut(s) 78, 1379, 1521
BmrI ACTGGG 2 cut(s) 507, 1035
BmsI GCATC 3 cut(s) 228, 235, 320
BmuI ACTGGG 2 cut(s) 507, 1035
BpiI GAAGAC 1 cut(s) 968
BpmI CTGGAG 3 cut(s) 170, 1084, 1130
Bpu10I CCTNAGC 1 cut(s) 1434
Bpu14I TTCGAA 1 cut(s) 26
BpuEI CTTGAG 1 cut(s) 533
BsaAI YACGTR 2 cut(s) 142, 274
BsaBI GATNNNNATC 2 cut(s) 255, 1778
BsaHI GRCGYC 1 cut(s) 1193
BsaJI CCNNGG 1 cut(s) 1378
BsaWI WCCGGW 2 cut(s) 638, 979
BsaXI ACNNNNNCTCC 4 cut(s) 168, 198, 1478, 1508
Bsc4I CCNNNNNNNGG 3 cut(s) 1091, 1750, 1776
Bse1I ACTGG 4 cut(s) 502, 1030, 1101, 1113
Bse3DI GCAATG 1 cut(s) 1374
Bse8I GATNNNNATC 2 cut(s) 255, 1778
BseAI TCCGGA 2 cut(s) 638, 979
BseBI CCWGG 3 cut(s) 78, 1379, 1521
BseDI CCNNGG 1 cut(s) 1378
BseGI GGATG 6 cut(s) 243, 335, 560, 1181, 1297, 1565
BseJI GATNNNNATC 2 cut(s) 255, 1778
BseLI CCNNNNNNNGG 3 cut(s) 1091, 1750, 1776
BseMI GCAATG 1 cut(s) 1374
BseMII CTCAG 5 cut(s) 725, 772, 1044, 1394, 1425
BseNI ACTGG 4 cut(s) 502, 1030, 1101, 1113
BseRI GAGGAG 1 cut(s) 184
BseSI GKGCMC 1 cut(s) 1769
BseXI GCAGC 5 cut(s) 593, 711, 786, 1376, 1639
BseYI CCCAGC 1 cut(s) 1791
BsgI GTGCAG 1 cut(s) 943
BshFI GGCC 3 cut(s) 76, 1095, 1210
BshNI GGYRCC 1 cut(s) 1192
BsiHKAI GWGCWC 1 cut(s) 1034
BsiHKCI CYCGRG 1 cut(s) 1613
BsiSI CCGG 2 cut(s) 639, 980
BslFI GGGAC 1 cut(s) 901
BslI CCNNNNNNNGG 3 cut(s) 1091, 1750, 1776
BsmAI GTCTC 4 cut(s) 166, 671, 1411, 1481
BsmFI GGGAC 1 cut(s) 901
BsmI GAATGC 2 cut(s) 777, 1007
BsnI GGCC 3 cut(s) 76, 1095, 1210
BsoBI CYCGRG 1 cut(s) 1613
Bsp119I TTCGAA 1 cut(s) 26
Bsp1286I GDGCHC 2 cut(s) 1034, 1769
Bsp13I TCCGGA 2 cut(s) 638, 979
Bsp1407I TGTACA 2 cut(s) 265, 536
Bsp143I GATC 5 cut(s) 11, 256, 712, 1711, 1779
BspACI CCGC 2 cut(s) 1580, 1645
BspANI GGCC 3 cut(s) 76, 1095, 1210
BspCNI CTCAG 5 cut(s) 726, 771, 1045, 1395, 1426
BspEI TCCGGA 2 cut(s) 638, 979
BspLI GGNNCC 3 cut(s) 424, 1194, 1220
BspMAI CTGCAG 2 cut(s) 729, 1366
BspMI ACCTGC 1 cut(s) 1369
BspPI GGATC 2 cut(s) 251, 1787
BspT104I TTCGAA 1 cut(s) 26
BspT107I GGYRCC 1 cut(s) 1192
BsrDI GCAATG 1 cut(s) 1374
BsrGI TGTACA 2 cut(s) 265, 536
BsrI ACTGG 4 cut(s) 502, 1030, 1101, 1113
BssECI CCNNGG 1 cut(s) 1378
BssMI GATC 5 cut(s) 11, 256, 712, 1711, 1779
BssNAI GTATAC 2 cut(s) 139, 1451
BssNI GRCGYC 1 cut(s) 1193
Bst1107I GTATAC 2 cut(s) 139, 1451
Bst2UI CCWGG 3 cut(s) 78, 1379, 1521
Bst4CI ACNGT 1 cut(s) 1576
Bst6I CTCTTC 3 cut(s) 52, 223, 1521
BstACI GRCGYC 1 cut(s) 1193
BstAUI TGTACA 2 cut(s) 265, 536
BstBAI YACGTR 2 cut(s) 142, 274
BstBI TTCGAA 1 cut(s) 26
BstC8I GCNNGC 2 cut(s) 1599, 1603
BstDEI CTNAG 6 cut(s) 656, 734, 758, 1053, 1403, 1434
BstENI CCTNNNNNAGG 1 cut(s) 1748
BstF5I GGATG 6 cut(s) 243, 335, 560, 1181, 1297, 1565
BstH2I RGCGCY 1 cut(s) 1196
BstHHI GCGC 2 cut(s) 1195, 1631
BstKTI GATC 5 cut(s) 14, 259, 715, 1714, 1782
BstMAI GTCTC 4 cut(s) 166, 671, 1411, 1481
BstMBI GATC 5 cut(s) 11, 256, 712, 1711, 1779
BstMWI GCNNNNNNNGC 4 cut(s) 92, 187, 871, 1628
BstNI CCWGG 3 cut(s) 78, 1379, 1521
BstNSI RCATGY 1 cut(s) 391
BstSCI CCNGG 3 cut(s) 76, 1377, 1519
BstSFI CTRYAG 2 cut(s) 725, 1362
BstSLI GKGCMC 1 cut(s) 1769
BstSNI TACGTA 1 cut(s) 142
BstV1I GCAGC 5 cut(s) 593, 711, 786, 1376, 1639
BstV2I GAAGAC 1 cut(s) 968
BstXI CCANNNNNNTGG 3 cut(s) 1108, 1556, 1593
BstZ17I GTATAC 2 cut(s) 139, 1451
BsuRI GGCC 3 cut(s) 76, 1095, 1210
BtrI CACGTC 1 cut(s) 1764
BtsCI GGATG 6 cut(s) 243, 335, 560, 1181, 1297, 1565
BtsI GCAGTG 1 cut(s) 1313
BtsIMutI CAGTG 3 cut(s) 495, 1108, 1313
BveI ACCTGC 1 cut(s) 1369
Cac8I GCNNGC 2 cut(s) 1599, 1603
CaiI CAGNNNCTG 1 cut(s) 733
CfoI GCGC 2 cut(s) 1195, 1631
Cfr13I GGNCC 1 cut(s) 595
Csp6I GTAC 3 cut(s) 266, 537, 1692
CviAII CATG 6 cut(s) 166, 326, 388, 646, 784, 1167
CviQI GTAC 3 cut(s) 266, 537, 1692
DdeI CTNAG 6 cut(s) 656, 734, 758, 1053, 1403, 1434
DinI GGCGCC 1 cut(s) 1194
DpnI GATC 5 cut(s) 13, 258, 714, 1713, 1781
DpnII GATC 5 cut(s) 11, 256, 712, 1711, 1779
DraI TTTAAA 1 cut(s) 945
EaeI YGGCCR 1 cut(s) 1208
Eam1104I CTCTTC 3 cut(s) 52, 223, 1521
EarI CTCTTC 3 cut(s) 52, 223, 1521
Eco105I TACGTA 1 cut(s) 142
Eco47I GGWCC 1 cut(s) 595
Eco57I CTGAAG 2 cut(s) 542, 638
Eco88I CYCGRG 1 cut(s) 1613
EcoNI CCTNNNNNAGG 1 cut(s) 1748
EcoRI GAATTC 1 cut(s) 312
EcoRII CCWGG 3 cut(s) 76, 1377, 1519
EgeI GGCGCC 1 cut(s) 1194
EheI GGCGCC 1 cut(s) 1194
FaeI CATG 6 cut(s) 169, 329, 391, 649, 787, 1170
FalI AAGNNNNNCTT 2 cut(s) 7, 39
FaqI GGGAC 1 cut(s) 901
FatI CATG 6 cut(s) 165, 325, 387, 645, 783, 1166
FauI CCCGC 1 cut(s) 1638
FauNDI CATATG 1 cut(s) 1784
FblI GTMKAC 4 cut(s) 20, 65, 138, 1450
Fnu4HI GCNGC 6 cut(s) 607, 725, 775, 1365, 1580, 1653
FokI GGATG 6 cut(s) 250, 342, 547, 1188, 1304, 1552
Fsp4HI GCNGC 6 cut(s) 607, 725, 775, 1365, 1580, 1653
FspBI CTAG 3 cut(s) 42, 113, 1670
GlaI GCGC 2 cut(s) 1194, 1630
GluI GCNGC 6 cut(s) 607, 725, 775, 1365, 1580, 1653
GsaI CCCAGC 1 cut(s) 1795
GsuI CTGGAG 3 cut(s) 170, 1084, 1130
HaeII RGCGCY 1 cut(s) 1196
HaeIII GGCC 3 cut(s) 76, 1095, 1210
HapII CCGG 2 cut(s) 639, 980
HhaI GCGC 2 cut(s) 1195, 1631
Hin1I GRCGYC 1 cut(s) 1193
Hin1II CATG 6 cut(s) 169, 329, 391, 649, 787, 1170
Hin6I GCGC 2 cut(s) 1193, 1629
HinP1I GCGC 2 cut(s) 1193, 1629
HincII GTYRAC 2 cut(s) 66, 385
HindII GTYRAC 2 cut(s) 66, 385
HindIII AAGCTT 2 cut(s) 524, 872
HinfI GANTC 7 cut(s) 370, 730, 763, 797, 1372, 1513, 1673
HpaII CCGG 2 cut(s) 639, 980
HphI GGTGA 2 cut(s) 548, 1074
Hpy166II GTNNAC 9 cut(s) 21, 66, 139, 266, 385, 556, 1106, 1358, 1451
Hpy188I TCNGA 9 cut(s) 196, 618, 735, 850, 1023, 1291, 1404, 1554, 1810
Hpy8I GTNNAC 9 cut(s) 21, 66, 139, 266, 385, 556, 1106, 1358, 1451
HpyAV CCTTC 4 cut(s) 42, 95, 166, 413
HpyCH4III ACNGT 1 cut(s) 1576
HpyCH4IV ACGT 4 cut(s) 141, 273, 381, 1763
HpyF10VI GCNNNNNNNGC 4 cut(s) 92, 187, 871, 1628
HpyF3I CTNAG 6 cut(s) 656, 734, 758, 1053, 1403, 1434
HpySE526I ACGT 4 cut(s) 141, 273, 381, 1763
Hsp92I GRCGYC 1 cut(s) 1193
Hsp92II CATG 6 cut(s) 169, 329, 391, 649, 787, 1170
HspAI GCGC 2 cut(s) 1193, 1629
KasI GGCGCC 1 cut(s) 1192
Kpn2I TCCGGA 2 cut(s) 638, 979
Kzo9I GATC 5 cut(s) 11, 256, 712, 1711, 1779
LmnI GCTCC 2 cut(s) 652, 1743
Lsp1109I GCAGC 5 cut(s) 593, 711, 786, 1376, 1639
LweI GCATC 3 cut(s) 228, 235, 320
MaeI CTAG 3 cut(s) 42, 113, 1670
MaeII ACGT 4 cut(s) 141, 273, 381, 1763
MaeIII GTNAC 4 cut(s) 1080, 1265, 1570, 1637
MalI GATC 5 cut(s) 13, 258, 714, 1713, 1781
MboI GATC 5 cut(s) 11, 256, 712, 1711, 1779
MboII GAAGA 8 cut(s) 69, 240, 379, 631, 871, 968, 1302, 1508
MfeI CAATTG 1 cut(s) 1591
MhlI GDGCHC 2 cut(s) 1034, 1769
MlsI TGGCCA 1 cut(s) 1210
MluNI TGGCCA 1 cut(s) 1210
Mly113I GGCGCC 1 cut(s) 1193
MlyI GAGTC 3 cut(s) 724, 772, 1507
MmeI TCCRAC 3 cut(s) 573, 978, 1329
Mox20I TGGCCA 1 cut(s) 1210
MroI TCCGGA 2 cut(s) 638, 979
MscI TGGCCA 1 cut(s) 1210
MslI CAYNNNNRTG 3 cut(s) 782, 1165, 1554
Msp20I TGGCCA 1 cut(s) 1210
MspI CCGG 2 cut(s) 639, 980
MspR9I CCNGG 3 cut(s) 78, 1379, 1521
MunI CAATTG 1 cut(s) 1591
Mva1269I GAATGC 2 cut(s) 777, 1007
MvaI CCWGG 3 cut(s) 78, 1379, 1521
MwoI GCNNNNNNNGC 4 cut(s) 92, 187, 871, 1628
NarI GGCGCC 1 cut(s) 1193
NdeI CATATG 1 cut(s) 1784
NdeII GATC 5 cut(s) 11, 256, 712, 1711, 1779
NlaIII CATG 6 cut(s) 169, 329, 391, 649, 787, 1170
NlaIV GGNNCC 3 cut(s) 424, 1194, 1220
NmuCI GTSAC 2 cut(s) 1080, 1265
NspI RCATGY 1 cut(s) 391
NspV TTCGAA 1 cut(s) 26
PciI ACATGT 1 cut(s) 387
PctI GAATGC 2 cut(s) 777, 1007
PfeI GAWTC 4 cut(s) 370, 797, 1372, 1673
PfoI TCCNGGA 1 cut(s) 1519
PkrI GCNGC 6 cut(s) 608, 726, 776, 1366, 1581, 1654
PleI GAGTC 3 cut(s) 724, 771, 1507
PluTI GGCGCC 1 cut(s) 1196
PpsI GAGTC 3 cut(s) 724, 771, 1507
Ppu21I YACGTR 2 cut(s) 142, 274
PscI ACATGT 1 cut(s) 387
PshBI ATTAAT 2 cut(s) 354, 918
PsiI TTATAA 1 cut(s) 1500
Psp6I CCWGG 3 cut(s) 76, 1377, 1519
PspFI CCCAGC 1 cut(s) 1791
PspGI CCWGG 3 cut(s) 76, 1377, 1519
PspN4I GGNNCC 3 cut(s) 424, 1194, 1220
PspPI GGNCC 1 cut(s) 595
PstI CTGCAG 2 cut(s) 729, 1366
PstNI CAGNNNCTG 1 cut(s) 733
RsaI GTAC 3 cut(s) 267, 538, 1693
RsaNI GTAC 3 cut(s) 266, 537, 1692
RseI CAYNNNNRTG 3 cut(s) 782, 1165, 1554
SalI GTCGAC 1 cut(s) 64
SatI GCNGC 6 cut(s) 607, 725, 775, 1365, 1580, 1653
Sau3AI GATC 5 cut(s) 11, 256, 712, 1711, 1779
Sau96I GGNCC 1 cut(s) 595
SchI GAGTC 3 cut(s) 724, 772, 1507
ScrFI CCNGG 3 cut(s) 78, 1379, 1521
SduI GDGCHC 2 cut(s) 1034, 1769
SfaNI GCATC 3 cut(s) 228, 235, 320
SfcI CTRYAG 2 cut(s) 725, 1362
SfoI GGCGCC 1 cut(s) 1194
SfuI TTCGAA 1 cut(s) 26
SinI GGWCC 1 cut(s) 595
SmiI ATTTAAAT 1 cut(s) 945
SmiMI CAYNNNNRTG 3 cut(s) 782, 1165, 1554
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
SnaBI TACGTA 1 cut(s) 142
SpeI ACTAGT 1 cut(s) 41
SsiI CCGC 2 cut(s) 1580, 1645
SspDI GGCGCC 1 cut(s) 1192
SspMI CTAG 3 cut(s) 42, 113, 1670
StyD4I CCNGG 3 cut(s) 76, 1377, 1519
SwaI ATTTAAAT 1 cut(s) 945
TaaI ACNGT 1 cut(s) 1576
TaiI ACGT 4 cut(s) 144, 276, 384, 1766
TaqI TCGA 6 cut(s) 26, 38, 65, 770, 1445, 1749
TaqII GACCGA 1 cut(s) 453
TatI WGTACW 2 cut(s) 265, 536
TauI GCSGC 1 cut(s) 1582
TfiI GAWTC 4 cut(s) 370, 797, 1372, 1673
TscAI CASTG 3 cut(s) 502, 1108, 1320
TseFI GTSAC 2 cut(s) 1080, 1265
TseI GCWGC 5 cut(s) 606, 724, 774, 1364, 1652
Tsp45I GTSAC 2 cut(s) 1080, 1265
TspDTI ATGAA 7 cut(s) 24, 165, 800, 872, 1149, 1385, 1431
TspGWI ACGGA 4 cut(s) 104, 845, 1007, 1558
TspRI CASTG 3 cut(s) 502, 1108, 1320
VpaK11BI GGWCC 1 cut(s) 595
VspI ATTAAT 2 cut(s) 354, 918
XagI CCTNNNNNAGG 1 cut(s) 1748
XapI RAATTY 5 cut(s) 312, 454, 946, 1066, 1803
XbaI TCTAGA 1 cut(s) 1669
XceI RCATGY 1 cut(s) 391
XmiI GTMKAC 4 cut(s) 20, 65, 138, 1450
XspI CTAG 3 cut(s) 42, 113, 1670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.