Rw1G035840

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000067
Physical Location & Seq
Forward (+)
63616075 .. 63622859
6785 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G035840.1

Sequence Viewer

Length: 5892 bp
ATGGCTTCTTCTTCATCTTCATCGGGCGGCCCCTGTAAGTATGATGTCTTCCTCAGTTTTAGAGGAGCAGACACCCGCAAGATCTTCGCCGGCCATCTTTACACAGCTCTGAAACTGAAAGCAATCCACACCTTCATCGATTCTGAAGAGCTCGAAAAAGGCAACAAAATTTCAGAGCTACTCACATGTATTGAGGAATCAAGGCTTTCGATTGTCGTTTTATCTCAAAACTATGCCTCTTCCACTTGGTGCTTGAAAGAGCTCGTCAAAATCCTGGATTGCATGGATACGAAGAAGCAGATGGTGCTGCCAGTCTTCTACGAAGTGGATCCCTCTGATCTACGGAAAATCCAAGGAAAATATGCAAAGGCTTTTGCCGAACATGGATTAAAGCCTGACCTCGACCAGAAAGAGTTGGAGAGCTGGAGGTCCGCTGTAATTAGAGTTGCGGGTCTAATTGGCTGGGACTCGCGAGATTATACGGATGACAGCAAGCTTATTGAGGAAATTGTTGCAGATACTCATAGAAAGCTGATCCAAACATCGTCCAATAAAGACAATGGCTTGGTTGGAATGGAGTCCCACATTACAGAAATGATGTCAAGATTATGTCTTCAGGTGAATGATGTTCGTGTTGTTGGAATATGGGGTATGGGTGGTCTAGGAAAAACAACCATTGCTAGAGCTGTTTTCAATGAAATTGCTTGGCAATTTGAACACTCCTGCTTTCTTGATAATGTCAAACAAGCTTTTAAAGACAATCTTGAAGTAAAAATGCAGGAAAAACTTCTGTACAGATTGTTGAATGAAAAGGTGAAGAGTGTAGATTTGAACAGAGGTAGGAATATGATAATGGAAAGGCTGGGTAGGAAAAAAGTCTTAGTTGTTCTTGATGATGTGGAAACTGTTGCCCAAATTGAAGACTTGCTTGGTAAACTACATGCATTTGGGGAGGGAAGTAGAGTCATTGTGACAACTAGAAATAAGCAATCACTCAGTAGAGTTGATGAGACATATCAGCCCACGATATTAAGTGATGATGAAGCTCTTGAGCTGTTTATGAAATATGCCTTCACAAGAAATCAACCCAATGGAGACTATGATCATCTCTCAAGGCATGCCATAGAATATTCTAAAGGTTTGCCTTTAGCACTTAAAGTCTTGGGGGCTTTTCTTGATAACAAAAGTGTGCGTGAATGGGAAGCAGTGTTGGAGAAATTGAAGAAAATCCCACAAATAGGAATTCAGGATGTGCTTAGAACAAGCATTGATGGACTAGACGATTTTGATAGGGACATATTTCTAGATATTGCATGTCTCTTTAGGGAATGGAACATAGATTTTGTAACAGAATTTCTAGACAGTTGTGGCTTCTATCCCCGCAATGGATTAAGGGTTCTAGTTGACAGAGCACTCATAACTTTGTCATATTACAGAGTCGACATGCATGATTTACTACAAGAAATGGGTAGAGAAATCGTCCGCCTAGAATCTACTAAAGAACCTGGGAAGCGCAGTAGGTTGTGGAGTTATGAAGATGTTAATCGTGTATTAACTCAAAATACGGCTACAGATACAGTTGAATGCATAGTGCTTGACTTGTCGAACTCAGAAGTTGACCTATGCATAAATACTGGAGCCTTTGTACGAATGACGAAGCTAAGACTACTCATAATCTATTATTCATTCTACACCATATTTGATGATAAAGATTATTGGGATTTTGAGGATATGTGCTCAGTCTATGGCTGTGAACAACTCTCGAGTGGGGACTTCGAGTTTCTTTCTCAAGAATTGAGGTTACTCTTATGGCATGGATGCCCGCTTAAGTCTTTGCCATCAACCTTTATCCCGAAGAATCTTGTTCAACTTGACATGCGTGCTAGTCACATTCAAGAACTTTGGGAAGGAATTAAGCCCCTACAAAATTTGAAAGTCATCAAGTTAAGTTACTGTCAATACCTTGTCAAAATCCCCGACTTATCTGAGGCGATAAATCTTGAGAAACTACTTCTTGCTGGCTGTTCAAGTTTATTTGAGGTTCATTCGTCCATTTCTGCTCTTCAAAACCTTCATTTATTGGATCTAAACAGGTGCAAAAAACTTAAGATTCTCCCAAGCTGCGTTCATATGAAATCTCTACGGGCCCTTAATCTTTCGGACTGCTCAAGTCTTGAAAAGTTTCCGGAGATTTCAGGAATCATGAAGGAGATATCAGAGATTCGTTTTGATGGGACTGCAATTAAAGAACTGCCCTCATCAATTGATCTGCTTCTGGGGATTAAAAGGTTAAGCATGAGGAACTGCAAAAGCCTTGTCTTTCTTCCGGACAGTTTCTGTAATTTGCCAGCCCTTACACATCTGGATCTCACAGGTTGCACAGAACTTAAGACTCTATCAACCAGTTTTCATATGAGTTCTCTTCAGAGCCTCAACCTCGATGGTTGCTCAAGTCTTGAAAAGTTTCCGGAGATTTCAGGAATCATGAAGGAGATATCAGAGATTCGTTTTGATGGGACTGCAATTAAAGAACTGCCCTCATCAATTGATCTGCTTCTGGGGATTAAAAGGTTAAGCATGAGGAACTGCAAAAGCCTTGTCTTTCTTCCGGACAGTATCTGTAATTTGCCAGCCCTTACACATCTGGATCTCACAGGTTGCACAGAACTTAAGACTCTATCAACCAGTTTTCATATGAGTTCTCTTCAGAGCCTCAACCTTGATGGTTGCTCAAGTCTTGAAAAGTTTCCGAAGATTTCAGGAATCATGAAGAAGCTACCAGAGCTTCGTTTAGATGGGACCGCAATTAAAGGACTGCCCTCATCAATTGATCTGCTTCTGGGGATTAAAAGGTTAAGCATGAGGAACTGCAAAAGCCTTGTCTTTCTTCCGGACAGTGTCTGTAATTTGCCAGCCCTTACACATCTGGATCTCACAGGTTGCACAGAACTTAAGACTCTATCAACCAGCTTTCATATGAGTTCTCTTCAGAGCCTCAACCTCGATGGCTGCTCAAGTCTTGAAAAGTTTCCGGAGATTTCAGGAATCATGAAGGAGCTATCAGAGCTTTTTTTAGATGAGACCGCAATTAAAGGACTGCCCTCATCAATTGATCTGCTTGAGGGGCTTAAAGTATTAAGCATGAGGAACTGCAAAAGCCTTGTCTTTCTTCCGGACAGTATCTGTAATTTGCCAGCCCTTACACATCTGGATCTCACAGGTTGCACAGAACTTAAGACTCTATCAACCAGCTTTCATATGAGTTCTCTTCAGAGCCTCAACCTCGATGGTTGCTCAAGTCTTGAAAAGTTTCCGGAGATTTCAGGAATCATGAAGGAGCTACTGGAGCTTCGTTTTGATGGGACTGCAATGAAAGAACTGCCCTCATCAATTGATCTGCTTCTGGGGATTGAAAGCTTAAGTATGAGGAACTGCAAAGGCCTTGAATTTCTTCCCGACAGTATCTGTAATTTGGCAGACCTTATACATCTGGATCTGTCTGGGTGCTCAGTACTTCATAACTTGCCTGAGAAATTGGGGGACTTAAAATCCTTGAGGTACCTGAAAGTATTGGGAAGTGGCATAGAAAGTTTCCCATTTTCTGTCCGCACGAACAAGAAGTTGGTGAGATCTTCATTTCATGGATGTGAAGAAATGTCGGAACCTTTTTCATCATCGTCGTCACTAATCAGATATTATAGTTTCTCTTGTCTGGTTCACCTTGATTTAAGTGAATGGAATATGTTGGAATTTTCGGATGCTTTTGCGCATTCAATTGATATGCTTTTGGGGATTAAAAGGTTAAGTATGAGGAACTGCAAAGGCCTTGAATTTCTTCCTGACAATATCTGTAATTTGCCAACCCTTAAACATCTGGATCTCACAGGTTGCACAGAACTTAAGACTCTATCAACCAGTTTTCATATGAGTTCGCTTCAGAGCTTCAACCTCGATGGTTGCTCAAGTCTTGAAAAGTTTCCAGAGATTTCAGGAATCATGAAGGAGCTACTGGAGCTTCGTTTTGATGGGACTGCAATTAAAGAACTGCCCTCATCAATTGATCTGCTTCTGGGGATTGAAAGCTTAAGTATGAGGAACTGCAAAGGCCTTGAATTTCTTCCCGACAGTATCTGTAATTTGGCAGACCTTATACATCTGGATCTGTCTGGGTGCTCAGTACTTCATAACTTGCCTGAGAAATTGGGGGACTTAAAATCCTTGAGGTACCTGAAAGTATTGGGAAGTGGCATAGAAAATTTCCCATTTTCTGTCCGCACGAACAAGAAGTTGGTGAGATCTTCATTTCATGGATGTGAAGAAATGTCGGAACCTTTTTCATCATCGTCATCACTAATCAGATATTATAGTTTCTCTTGTCTGGTTCACCTTGATTTAAGTGAATGGAATATGTTGGAATTTTCGGATGCTTTTGCGCATTCAATTGATCTGCTTCTGGGGATTAAAAGGTTAAGTATGAGGAACTGCAAAGGCCTTGAATTTCTTCCTGACAATATCTGTAATTTGCCAACCCTTAAACATCTGGATCTCACAGGTTGCACAGAACTTAAGACTCTATCAACCAGTTTTCATATGAGTTCGCTTCAGAGCCTCAACCTCGATGGTTGCTCAAGTCTTGAAAAGTTTCCGGAGATTTCAGGAATCATGAAGGAGATATCAGAGATTCGTTTTGATGGGACTGCAATTAAAGAACTGCCCTCATCAATTAATCTGCTTCTGGGGATTAAAAGGCTAAGCATGAGGAACTGCAAAAGCCTTGCCTTTCTTCCGGACAGTATCTGTAATTTGCCAGCCCTTACACATCTGGATCTCACAGGTTGCACAGAACTTAAGACTCTATCAACCGGTTTTCATATGAGTTCTCTTCAGAGCTTCAACCTCGATGGTTGCTCAAGCCTTGAAAAGTTTCCGGAGATTTCAGGAATCATGAAGGAGCTACTGGAGCTTCGTTTTGATGGGACTGCAATTAAAGAACTGCCCTCATCAATTGATCTGCTTCTGGGAATTGAAAGCTTAAGTTTGAGGAACTGCAAAGGCCTTGAATTTCTTCCCGACAGTATCTGTAATTTGGCAGACCTTATACATCTGGATCTGTCTGGGTGCTCAGTACTTCATAACTTGCCTGAGAAATTGGGGGACTTAAAATCCTTGAGGTACCTGAAAGTATTGGGAAGTGGCATAGAACATTTCCCATTTTCTGTCCGCACGAACAAGAAGTTGGTGAGATCTTCATTTCATGGATGTGAAGAAATGTCGGAACCTTTTTCATCATCGTCATCACTAATCAGATATTATAGTTTCTCTTGTCTGGTTCACCTTGATTTAAGTGAATGGAATATGTTGGAATTTTCGGATGCTTTTGCGCATTCAATTGATATGCTTCTGGGGATTAAAAGGTTAAGTATGAGGAACTGCAAAGGCCTTGAATTTCTTCCTGACAATATCTGTAATTTGCCAACCCTTAAACATCTGGATCTCACAGGTTGCACAGAACTTAAGACTCTACCAACCAGCATTCATATGATTTCTCTTCGGACCCTTAAGCTCAATGGCTGCTCAAATCTTGAAATGTTTCCGGAGATTGCAGGAATCATGAAGGAGCTATCAGAGCTTCTTTTAGATGAGACTGCAATTAAAGGACTGCCCTCATCAATTAATCTGCTTCTGGGGATTAAAAGGTTAAGCATGAGGAACTGCAAAAGCCTTGAATTTCTTCCGGACAGTATCTGTGATTTGCCAGCCCTTACACATCTGGATCTCACAGGTTGCACAGGACTTAAGACTCTATCAACCTGTTTTCGATGGTTGCTCAAGTCTAGAAAAGTTTCCGGAGATTTCAGGAATCATGAAGGAGCTACTGGAGCTTCGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1963

Amino Acids

219.83

Weight (kDa)

5.8

Isoelectric Point (pI)

45.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 13 - 191 4.5e-51 TIR domain
TIR_2 PF13676 16 - 118 1.1e-13 TIR domain
NB-ARC PF00931 194 - 361 1.6e-23 NB-ARC domain
WHD_ROQ1 PF23282 430 - 497 9.9e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 702 - 818 6.6e-06 Leucine-rich repeat region
LRR_13 PF23286 711 - 801 3.5e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 805 - 893 1.8e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 899 - 989 4.5e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 993 - 1083 5.3e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 1018 - 1117 3.5e-06 Leucine-rich repeat region
LRR_13 PF23286 1087 - 1173 3.5e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 1093 - 1191 8.7e-08 Leucine-rich repeat region
LRR_13 PF23286 1309 - 1395 3.4e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 1334 - 1413 5.3e-08 Leucine-rich repeat region
LRR_13 PF23286 1531 - 1626 2.5e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 1625 - 1711 3.8e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 1649 - 1729 2.5e-08 Leucine-rich repeat region
LRR_13 PF23286 1848 - 1943 6e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 3 cut(s) 3768, 4434, 5382
Acc65I GGTACC 3 cut(s) 3558, 4224, 5172
AccB1I GGYRCC 3 cut(s) 3558, 4224, 5172
AccI GTMKAC 1 cut(s) 1440
AccII CGCG 1 cut(s) 472
AcoI YGGCCR 1 cut(s) 91
AcuI CTGAAG 9 cut(s) 165, 599, 2408, 2690, 2972, 3254, 3920, 4586, 4868
AdeI CACNNNGTG 1 cut(s) 249
AfaI GTAC 8 cut(s) 794, 1647, 3513, 3560, 4179, 4226, 5127, 5174
AfiI CCNNNNNNNGG 2 cut(s) 1238, 1385
AflIII ACRYGT 1 cut(s) 185
AgeI ACCGGT 1 cut(s) 4862
AjnI CCWGG 2 cut(s) 273, 1504
AjuI GAANNNNNNNTTGG 8 cut(s) 912, 944, 3605, 3637, 4271, 4303, 5219, 5251
AloI GAACNNNNNNTCC 2 cut(s) 1380, 1412
Alw21I GWGCWC 7 cut(s) 153, 264, 1414, 1739, 3509, 4175, 5123
Alw26I GTCTC 5 cut(s) 1004, 1089, 1322, 3074, 5636
AlwNI CAGNNNCTG 9 cut(s) 2337, 2619, 2901, 3183, 3465, 4131, 4797, 5079, 5745
Ama87I CYCGRG 1 cut(s) 1762
AoxI GGCC 9 cut(s) 28, 91, 2145, 3439, 3823, 4105, 4489, 5053, 5437
ApaI GGGCCC 1 cut(s) 2149
ApeKI GCWGC 4 cut(s) 307, 2121, 3009, 5571
ArsI GACNNNNNNTTYG 8 cut(s) 2301, 2333, 2583, 2615, 2865, 2897, 3147, 3179
AseI ATTAAT 2 cut(s) 4725, 5673
AsiGI ACCGGT 1 cut(s) 4862
Asp718I GGTACC 3 cut(s) 3558, 4224, 5172
AspLEI GCGC 4 cut(s) 1515, 3769, 4435, 5383
AspS9I GGNCC 6 cut(s) 29, 429, 2145, 2146, 2799, 5553
AsuHPI GGTGA 8 cut(s) 631, 826, 3637, 3710, 4303, 4376, 5251, 5324
AvaI CYCGRG 1 cut(s) 1762
AvaII GGWCC 3 cut(s) 429, 2799, 5553
BaeGI GKGCMC 1 cut(s) 2149
BamHI GGATCC 1 cut(s) 328
BanI GGYRCC 3 cut(s) 3558, 4224, 5172
BanII GRGCYC 3 cut(s) 153, 264, 2149
BarI GAAGNNNNNNTAC 2 cut(s) 32, 64
BbsI GAAGAC 4 cut(s) 40, 307, 605, 927
Bbv12I GWGCWC 7 cut(s) 153, 264, 1414, 1739, 3509, 4175, 5123
BbvI GCAGC 4 cut(s) 294, 2108, 2996, 5558
BceAI ACGGC 1 cut(s) 1581
BcgI CGANNNNNNTGC 2 cut(s) 67, 101
BciT130I CCWGG 2 cut(s) 275, 1506
BciVI GTATCC 1 cut(s) 280
BclI TGATCA 1 cut(s) 1102
BcoDI GTCTC 5 cut(s) 1004, 1089, 1322, 3074, 5636
BfmI CTRYAG 1 cut(s) 1569
BfuI GTATCC 1 cut(s) 280
BglII AGATCT 4 cut(s) 81, 3629, 4295, 5243
BisI GCNGC 5 cut(s) 28, 308, 2122, 3010, 5572
BlpI GCTNAGC 1 cut(s) 4751
BlsI GCNGC 5 cut(s) 29, 309, 2123, 3011, 5573
BmcAI AGTACT 3 cut(s) 3513, 4179, 5127
Bme1390I CCNGG 2 cut(s) 275, 1506
Bme18I GGWCC 3 cut(s) 429, 2799, 5553
BmeT110I CYCGRG 1 cut(s) 1762
BmgT120I GGNCC 6 cut(s) 29, 429, 2145, 2146, 2799, 5553
BmrFI CCNGG 2 cut(s) 275, 1506
BmsI GCATC 4 cut(s) 1808, 3748, 4414, 5362
BpiI GAAGAC 4 cut(s) 40, 307, 605, 927
BpmI CTGGAG 5 cut(s) 445, 1656, 3365, 4031, 4979
Bpu1102I GCTNAGC 1 cut(s) 4751
Bsa29I ATCGAT 1 cut(s) 138
BsaBI GATNNNNATC 1 cut(s) 1542
BsaI GGTCTC 1 cut(s) 3074
BsaJI CCNNGG 2 cut(s) 352, 1505
BsaXI ACNNNNNCTCC 2 cut(s) 1629, 1659
Bsc4I CCNNNNNNNGG 2 cut(s) 1238, 1385
Bse118I RCCGGY 2 cut(s) 89, 4862
Bse3DI GCAATG 3 cut(s) 675, 1390, 3375
Bse8I GATNNNNATC 1 cut(s) 1542
BseBI CCWGG 2 cut(s) 275, 1506
BseCI ATCGAT 1 cut(s) 138
BseDI CCNNGG 2 cut(s) 352, 1505
BseGI GGATG 9 cut(s) 490, 1255, 1823, 3650, 3763, 4316, 4429, 5264, 5377
BseJI GATNNNNATC 1 cut(s) 1542
BseLI CCNNNNNNNGG 2 cut(s) 1238, 1385
BseMI GCAATG 3 cut(s) 675, 1390, 3375
BseRI GAGGAG 1 cut(s) 78
BseSI GKGCMC 1 cut(s) 2149
BseXI GCAGC 4 cut(s) 294, 2108, 2996, 5558
BseYI CCCAGC 2 cut(s) 462, 862
Bsh1236I CGCG 1 cut(s) 472
BshFI GGCC 9 cut(s) 30, 93, 2147, 3441, 3825, 4107, 4491, 5055, 5439
BshNI GGYRCC 3 cut(s) 3558, 4224, 5172
BshTI ACCGGT 1 cut(s) 4862
BshVI ATCGAT 1 cut(s) 138
BsiHKAI GWGCWC 7 cut(s) 153, 264, 1414, 1739, 3509, 4175, 5123
BsiHKCI CYCGRG 1 cut(s) 1762
BslI CCNNNNNNNGG 2 cut(s) 1238, 1385
BsmAI GTCTC 5 cut(s) 1004, 1089, 1322, 3074, 5636
BsmI GAATGC 5 cut(s) 1589, 3769, 4435, 5383, 5532
BsnI GGCC 9 cut(s) 30, 93, 2147, 3441, 3825, 4107, 4491, 5055, 5439
Bso31I GGTCTC 1 cut(s) 3074
BsoBI CYCGRG 1 cut(s) 1762
Bsp120I GGGCCC 1 cut(s) 2145
Bsp1286I GDGCHC 8 cut(s) 153, 264, 1414, 1739, 2149, 3509, 4175, 5123
Bsp1407I TGTACA 1 cut(s) 792
Bsp1720I GCTNAGC 1 cut(s) 4751
Bsp68I TCGCGA 1 cut(s) 472
BspANI GGCC 9 cut(s) 30, 93, 2147, 3441, 3825, 4107, 4491, 5055, 5439
BspDI ATCGAT 1 cut(s) 138
BspFNI CGCG 1 cut(s) 472
BspQI GCTCTTC 2 cut(s) 141, 2067
BspT107I GGYRCC 3 cut(s) 3558, 4224, 5172
BspTNI GGTCTC 1 cut(s) 3074
BsrDI GCAATG 3 cut(s) 675, 1390, 3375
BsrFI RCCGGY 2 cut(s) 89, 4862
BsrGI TGTACA 1 cut(s) 792
BssAI RCCGGY 2 cut(s) 89, 4862
BssECI CCNNGG 2 cut(s) 352, 1505
BssT1I CCWWGG 1 cut(s) 352
Bst2UI CCWGG 2 cut(s) 275, 1506
BstAPI GCANNNNNTGC 1 cut(s) 304
BstAUI TGTACA 1 cut(s) 792
BstF5I GGATG 9 cut(s) 490, 1255, 1823, 3650, 3763, 4316, 4429, 5264, 5377
BstFNI CGCG 1 cut(s) 472
BstHHI GCGC 4 cut(s) 1515, 3769, 4435, 5383
BstMAI GTCTC 5 cut(s) 1004, 1089, 1322, 3074, 5636
BstMWI GCNNNNNNNGC 9 cut(s) 304, 2782, 3064, 3124, 3346, 4012, 4960, 5626, 5879
BstNI CCWGG 2 cut(s) 275, 1506
BstNSI RCATGY 6 cut(s) 189, 944, 1121, 1317, 1447, 1879
BstSCI CCNGG 2 cut(s) 273, 1504
BstSFI CTRYAG 1 cut(s) 1569
BstSLI GKGCMC 1 cut(s) 2149
BstUI CGCG 1 cut(s) 472
BstV1I GCAGC 4 cut(s) 294, 2108, 2996, 5558
BstV2I GAAGAC 4 cut(s) 40, 307, 605, 927
Bsu15I ATCGAT 1 cut(s) 138
BsuI GTATCC 1 cut(s) 280
BsuRI GGCC 9 cut(s) 30, 93, 2147, 3441, 3825, 4107, 4491, 5055, 5439
BsuTUI ATCGAT 1 cut(s) 138
BtsCI GGATG 9 cut(s) 490, 1255, 1823, 3650, 3763, 4316, 4429, 5264, 5377
BtsI GCAGTG 1 cut(s) 1212
BtsIMutI CAGTG 2 cut(s) 1212, 2902
BtuMI TCGCGA 1 cut(s) 472
CaiI CAGNNNCTG 9 cut(s) 2337, 2619, 2901, 3183, 3465, 4131, 4797, 5079, 5745
CfoI GCGC 4 cut(s) 1515, 3769, 4435, 5383
Cfr10I RCCGGY 2 cut(s) 89, 4862
Cfr13I GGNCC 6 cut(s) 29, 429, 2145, 2146, 2799, 5553
ClaI ATCGAT 1 cut(s) 138
Csp6I GTAC 8 cut(s) 793, 1646, 3512, 3559, 4178, 4225, 5126, 5173
CspAI ACCGGT 1 cut(s) 4862
CviQI GTAC 8 cut(s) 793, 1646, 3512, 3559, 4178, 4225, 5126, 5173
DraI TTTAAA 1 cut(s) 754
DraIII CACNNNGTG 1 cut(s) 249
EaeI YGGCCR 1 cut(s) 91
EciI GGCGGA 1 cut(s) 1472
Ecl136II GAGCTC 2 cut(s) 151, 262
Eco130I CCWWGG 1 cut(s) 352
Eco147I AGGCCT 6 cut(s) 3441, 3825, 4107, 4491, 5055, 5439
Eco24I GRGCYC 3 cut(s) 153, 264, 2149
Eco31I GGTCTC 1 cut(s) 3074
Eco32I GATATC 3 cut(s) 2214, 2496, 4674
Eco47I GGWCC 3 cut(s) 429, 2799, 5553
Eco53kI GAGCTC 2 cut(s) 151, 262
Eco57I CTGAAG 9 cut(s) 165, 599, 2408, 2690, 2972, 3254, 3920, 4586, 4868
Eco88I CYCGRG 1 cut(s) 1762
EcoICRI GAGCTC 2 cut(s) 151, 262
EcoO109I RGGNCCY 1 cut(s) 2146
EcoRI GAATTC 1 cut(s) 1242
EcoRII CCWGG 2 cut(s) 273, 1504
EcoRV GATATC 3 cut(s) 2214, 2496, 4674
EcoT14I CCWWGG 1 cut(s) 352
EcoT22I ATGCAT 4 cut(s) 946, 1449, 1589, 1628
EcoT38I GRGCYC 3 cut(s) 153, 264, 2149
ErhI CCWWGG 1 cut(s) 352
FalI AAGNNNNNCTT 4 cut(s) 747, 779, 912, 944
FauI CCCGC 4 cut(s) 83, 442, 1388, 1830
FauNDI CATATG 9 cut(s) 2130, 2412, 2694, 2976, 3258, 3924, 4590, 4872, 5538
FbaI TGATCA 1 cut(s) 1102
FblI GTMKAC 1 cut(s) 1440
Fnu4HI GCNGC 5 cut(s) 28, 308, 2122, 3010, 5572
FokI GGATG 9 cut(s) 497, 1262, 1830, 3657, 3770, 4323, 4436, 5271, 5384
FriOI GRGCYC 3 cut(s) 153, 264, 2149
Fsp4HI GCNGC 5 cut(s) 28, 308, 2122, 3010, 5572
FspI TGCGCA 3 cut(s) 3768, 4434, 5382
GlaI GCGC 4 cut(s) 1514, 3768, 4434, 5382
GluI GCNGC 5 cut(s) 28, 308, 2122, 3010, 5572
GsaI CCCAGC 2 cut(s) 466, 866
GsuI CTGGAG 5 cut(s) 445, 1656, 3365, 4031, 4979
HaeIII GGCC 9 cut(s) 30, 93, 2147, 3441, 3825, 4107, 4491, 5055, 5439
HhaI GCGC 4 cut(s) 1515, 3769, 4435, 5383
Hin6I GCGC 4 cut(s) 1513, 3767, 4433, 5381
HinP1I GCGC 4 cut(s) 1513, 3767, 4433, 5381
HincII GTYRAC 3 cut(s) 1405, 1441, 1618
HindII GTYRAC 3 cut(s) 1405, 1441, 1618
HindIII AAGCTT 5 cut(s) 494, 747, 3415, 4081, 5029
HphI GGTGA 8 cut(s) 631, 826, 3637, 3710, 4303, 4376, 5251, 5324
Hpy166II GTNNAC 8 cut(s) 935, 1405, 1441, 1618, 1754, 3718, 4384, 5332
Hpy8I GTNNAC 8 cut(s) 935, 1405, 1441, 1618, 1754, 3718, 4384, 5332
Hpy99I CGWCG 1 cut(s) 3682
HpyF10VI GCNNNNNNNGC 9 cut(s) 304, 2782, 3064, 3124, 3346, 4012, 4960, 5626, 5879
HspAI GCGC 4 cut(s) 1513, 3767, 4433, 5381
KpnI GGTACC 3 cut(s) 3562, 4228, 5176
KroI GCCGGC 1 cut(s) 89
KroNI GCCGGC 1 cut(s) 91
Ksp22I TGATCA 1 cut(s) 1102
LguI GCTCTTC 2 cut(s) 141, 2067
Lsp1109I GCAGC 4 cut(s) 294, 2108, 2996, 5558
LweI GCATC 4 cut(s) 1808, 3748, 4414, 5362
MaeIII GTNAC 6 cut(s) 970, 1345, 1800, 1886, 1949, 3681
MhlI GDGCHC 8 cut(s) 153, 264, 1414, 1739, 2149, 3509, 4175, 5123
Mph1103I ATGCAT 4 cut(s) 946, 1449, 1589, 1628
MroNI GCCGGC 1 cut(s) 89
MslI CAYNNNNRTG 4 cut(s) 3645, 4311, 5259, 5537
MspA1I CMGCKG 1 cut(s) 434
MspR9I CCNGG 2 cut(s) 275, 1506
Mva1269I GAATGC 5 cut(s) 1589, 3769, 4435, 5383, 5532
MvaI CCWGG 2 cut(s) 275, 1506
MvnI CGCG 1 cut(s) 472
MwoI GCNNNNNNNGC 9 cut(s) 304, 2782, 3064, 3124, 3346, 4012, 4960, 5626, 5879
NaeI GCCGGC 1 cut(s) 91
NdeI CATATG 9 cut(s) 2130, 2412, 2694, 2976, 3258, 3924, 4590, 4872, 5538
NgoMIV GCCGGC 1 cut(s) 89
NmuCI GTSAC 3 cut(s) 970, 1886, 3681
NruI TCGCGA 1 cut(s) 472
NsbI TGCGCA 3 cut(s) 3768, 4434, 5382
NsiI ATGCAT 4 cut(s) 946, 1449, 1589, 1628
NspI RCATGY 6 cut(s) 189, 944, 1121, 1317, 1447, 1879
PaeI GCATGC 1 cut(s) 1121
PaeR7I CTCGAG 1 cut(s) 1762
PceI AGGCCT 6 cut(s) 3441, 3825, 4107, 4491, 5055, 5439
PciI ACATGT 1 cut(s) 185
PciSI GCTCTTC 2 cut(s) 141, 2067
PctI GAATGC 5 cut(s) 1589, 3769, 4435, 5383, 5532
PdiI GCCGGC 1 cut(s) 91
PflFI GACNNNGTC 1 cut(s) 2897
PfoI TCCNGGA 1 cut(s) 273
PinAI ACCGGT 1 cut(s) 4862
PkrI GCNGC 5 cut(s) 29, 309, 2123, 3011, 5573
PscI ACATGT 1 cut(s) 185
PshBI ATTAAT 2 cut(s) 4725, 5673
Psp124BI GAGCTC 2 cut(s) 153, 264
Psp6I CCWGG 2 cut(s) 273, 1504
PspFI CCCAGC 2 cut(s) 462, 862
PspGI CCWGG 2 cut(s) 273, 1504
PspOMI GGGCCC 1 cut(s) 2145
PspPI GGNCC 6 cut(s) 29, 429, 2145, 2146, 2799, 5553
PstNI CAGNNNCTG 9 cut(s) 2337, 2619, 2901, 3183, 3465, 4131, 4797, 5079, 5745
PsyI GACNNNGTC 1 cut(s) 2897
RruI TCGCGA 1 cut(s) 472
RsaI GTAC 8 cut(s) 794, 1647, 3513, 3560, 4179, 4226, 5127, 5174
RsaNI GTAC 8 cut(s) 793, 1646, 3512, 3559, 4178, 4225, 5126, 5173
RseI CAYNNNNRTG 4 cut(s) 3645, 4311, 5259, 5537
SacI GAGCTC 2 cut(s) 153, 264
SalI GTCGAC 1 cut(s) 1439
SapI GCTCTTC 2 cut(s) 141, 2067
SatI GCNGC 5 cut(s) 28, 308, 2122, 3010, 5572
Sau96I GGNCC 6 cut(s) 29, 429, 2145, 2146, 2799, 5553
ScaI AGTACT 3 cut(s) 3513, 4179, 5127
ScrFI CCNGG 2 cut(s) 275, 1506
SduI GDGCHC 8 cut(s) 153, 264, 1414, 1739, 2149, 3509, 4175, 5123
SfaNI GCATC 4 cut(s) 1808, 3748, 4414, 5362
SfcI CTRYAG 1 cut(s) 1569
Sfr274I CTCGAG 1 cut(s) 1762
SinI GGWCC 3 cut(s) 429, 2799, 5553
SlaI CTCGAG 1 cut(s) 1762
SmiMI CAYNNNNRTG 4 cut(s) 3645, 4311, 5259, 5537
SphI GCATGC 1 cut(s) 1121
SseBI AGGCCT 6 cut(s) 3441, 3825, 4107, 4491, 5055, 5439
SspI AATATT 1 cut(s) 1130
SstI GAGCTC 2 cut(s) 153, 264
StuI AGGCCT 6 cut(s) 3441, 3825, 4107, 4491, 5055, 5439
StyD4I CCNGG 2 cut(s) 273, 1504
StyI CCWWGG 1 cut(s) 352
TatI WGTACW 4 cut(s) 792, 3511, 4177, 5125
TauI GCSGC 1 cut(s) 30
TscAI CASTG 2 cut(s) 1212, 2902
TseFI GTSAC 3 cut(s) 970, 1886, 3681
TseI GCWGC 4 cut(s) 307, 2121, 3009, 5571
Tsp45I GTSAC 3 cut(s) 970, 1886, 3681
TspGWI ACGGA 2 cut(s) 358, 497
TspRI CASTG 2 cut(s) 1212, 2902
Tth111I GACNNNGTC 1 cut(s) 2897
VpaK11BI GGWCC 3 cut(s) 429, 2799, 5553
VspI ATTAAT 2 cut(s) 4725, 5673
XbaI TCTAGA 3 cut(s) 1303, 1357, 5834
XceI RCATGY 6 cut(s) 189, 944, 1121, 1317, 1447, 1879
XhoI CTCGAG 1 cut(s) 1762
XmiI GTMKAC 1 cut(s) 1440
ZrmI AGTACT 3 cut(s) 3513, 4179, 5127
Zsp2I ATGCAT 4 cut(s) 946, 1449, 1589, 1628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.