RchiOBHm_Chr1g0375941

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000067
Physical Location & Seq
Forward (+)
63224826 .. 63233110
8285 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59962

Sequence Viewer

Length: 4548 bp
ATGATGGCTTCTTCTTCATCTCCATCCGGCGATCCCTGTGAGTATGATGTCTTCCTCAGTTTTAGAGGAGAAGACACCCGCAAGATCTTCGCCGGCCATCTTTACAGAGCTCTGCAACACAAAGCAATCCGCGCCTTCATCGATTCTGAAGAGCTCGAAAAAGGCAACAATATTTCAGAGCTACTCACATATATTGAGAAATCAAGGCTTTCGATTGTCGTTTTATCTCGAAACTATGCATTTTCCACTTGGTGCTTAAAAGAGCTCGTCAAAATCCTGGATTGCATGGATAAGAAGAAGCAGATGGTGCTGCCTATCTTCTACGATGTGGATCCCTCTGATCTACGGAAAATCAAAGGAAAATATGCAGAAGCTTTTGCCAAACATGGAGAAAATCCTAACATTGACAAGGAAGAGGTGAAGAGCTGGAGGTCCTCTGTAAATAGAGTTGCGGATCTATCTGGCTGGGATTCGACCAAATATGAGGATGACGGCAAGCTTATTGAGGAAATTGTTGCAAATACTCATAGAAAGCTGATCCAAACATCGTCCAATAAAGACAATGGCTTGGTTGGAATGGATTCCCACATTACAGAAATGATGTCAAGATTATGTCTTCAGGTGAATGATGTTCGTGTTGTTGGAATATGGGGTATGGGCGGTCTAGGAAAAACAACCATTGCTAGAGCTGTTTTCGATGAAATTGCTCGGCAATTTGAACACTCCTGCTTTCTTGGTAATGTCAAAGAAGCTTTTAAAGACAAGCGTGAAGTAAAAAAAATGCAGCAAGAACTTCTGTTCAAATTGTTGAATGAAAAGGTAAAGAGTGTAGATTTGGACAAAGATAGGAATGTGATAATGGAAAGGCTGGGTAGGAAAAAAGTCTTAGTTGTTCTTGATGATGTGGAAACTGTTACCCAAATTGAACACTTGCTTGGTAAACCACATGCATTTGGGGAGGGAAGTAGAGTCATTGTGACAACTAGAAATAAGCAATCACTCAGTAGAGTTGATGAGACATATCACCCCACGATATTAAGTGATGAAGAAGCTCTTGAGCTGTTTATGAAATATGCCTTCACAAGAAATCAACCCAATGGAGACTATGATCATCTCTCAAGGCATGCCATAGAATATTCTAAAGGTTTGCCTTTAGCACTTAAAGTCTTGGGGGCTTTTCTTGATAACAAAAGTGTGCCTGAATGGGAAGCAGTGTTGGAGAAATTGAAGAAAATCCCACAAATAGAAATTCAGGATGTGCTTAGAACAAGCATTGTTGGACTAGACGATTTTGATAGGGACATATTTCTAGATATTGCATGTCTCTTTAGGGAATGGAACACAGATTTTGTAACAGAATTACTAGACAGTTGTGGCTTCTATCCCCGCAATGGATTAAGGGTTCTAGTTGACAGAGCACTCATAACTTTGTCATTTGACAGAGTCAACATGCATGATTTACTACAAGGAATGGGTAGAGAAATCGTCCGCCTAGAATCTCCTAGAGAACCTGGGAAGCGCAGTAGGTTGTGGAGTTATGAAGATGTTAATTGTGTATTAACTCAAAATATGGCTACGGATGCAGTTGAATGCTTAGTGCTTGACTTGTCGAACTCAGAAGTTGACCTATGCATAAATACTGGAGCCTTTGTACGAATGAAGAAGCTAAGACTACTCATAATCTATTATTCATTCTACACCATATTTGATGATTTTGAAGATGAAGATTATTCTGATTTTGAGGATATTTGCCCAGTCTATGGCTGTAAACAACTCTCGAGGGGGGACTTCGAGTTTCTTTCTCAAAAATTGAGGTTACTCATATGGCATGGATGCCCGCTTAAGTCTTTGCCATCAACCTTTATCCCGGAGAATCTTGTTCAGCTTGACATGCGTGCTAGTCACATTCGAGAACTTTGGGAAGGAATTAAGCCCCTACAAAATCTGAAAGTCATCAAGTTAAGTCACTGTCAATACCTTGTCAAAATCCCCGACTTAACTGAGGCCATAAAACTTGAGAGAGTACTTCTTGACGGCTGTTCAAGTTTATTTGAGGTTCATTCATCCATTTCTGCTCTTGAAAACCTTCATTTCGTGGATCTAAAGGGGTGCAAACAGCTTAAGATTCTCCCAAGCTGCATTCATATGAAATCTCTACGGGCACTTAAACTTTCGGGCTGCGCAAATCTTGAAAAGTTTCCAGAGATTGCAGATGTTATGATGGAGTTAAAAGAGCTTTATCTAGATGGGACTGCAGTTAAAGAACTACCCTCATCTATTAACAATCTCACTGGGCTTCGTGTTTTGGATCTCACAGATTGCACAGAACTTAAGACTCTATCAACCAGCATTCATATGCCTTATCTTCAGACCCTCGAACTCGGTGGCTGCTCAAGTCTTGAAAAGTTTCCGGAGATTTCAGGAATCATGAAGGAGCTAACAGTGCTTTGTTTAGATGAGACTGCAATTAAGGGACTTCCCTCGTCAATTGATCTACTTGAGGGGCTTGAAGAATTAAGCATGCAGAACTGCAAAAGCCTTGTCTCTCTTCCGGACAGTATCTGTAATTTGCCAGCCCTTAAACATCTGGATCTCACGGGTTGCACAGAACTTAAGACTCTATCAACCAGCTTTCATATGAGTTCTCTTCGGTACCTTTACCTCGATGGCTGCTCAAATCTTGAAAAGTTTCCGGAGATTTCAGGAATCATGAAGGAGCTACCAGAGCTTCGTTTAGATGAGACCGCAATTAAAGGACTTCCCTCATCAATTGATCATCTTGAGGGGCTTAAAGTATTGAGTATGCGGGACTGCAAAAGCCTTGAATTTCTTCCCGACAGTATCTGTAATTTGGCAGACCTGACACATTTGGATCTGTCTGGGTGCCCAGTATTTCATAACTTGCCTGAGAAATTGGGGGACTTAAAATCCTTACAGGTCCTTAACGTAAGGGATAGTGGTATAAAACAACTTCCATTGTCTATTCTACGTTTGGAGAAGCTTGTACGTGACGGATTATCATGTAGTGGATGTAAAGAGATGATCGCACGCTTGTCAGCATGGCCATCATCAATCGAAGGGCATTGTAGTTACTCTGTTCTGCTACATGTTGATTTAAGTGACTGCAATCTGTTGGAGTTATCGGATGGTATTGCTCATTTATATTCATTGAAAACATTAAAGCTGTGCCGAACCAACTTGGAGAGCTTACCTGTGGCAATGAATCGGCTTTGTTGTTTAACGCATCTTGAATTGGAAGCTTGCAGGAGGCTGAAATCAATACCAGAGCTTTCATCGAGTATAAATTACATAGATGCACATGATTGCACAGCTTTGGAAAAAGTTTCAATGCCAAAGCCTCAGGACAAGACTTATCATTTCTTTACATTTTCTAATTGCCTTCAGCTGGTACAAACAAATCTATTTACAGATATTGTGGAAAGACATTCACATTTGCAGGATAATTACCTGCGACCTCTTCAGTTGAATATGTCTCTTCCTGGGAGTGAAGTTCCCGATTGGTTCAACCATCAGAGTAGGGGATTTTCAGTGAGTGTGCAGCTACCACCAAATTGGTTTGACAGTAAGTTTTGGGGATTCGCTATCTGTGCTGTTCGTGTATGCCAGGGACTTATTGATGATACATCTCCTTTATCTGCTGCTTGTGTATGTACCTTCAAAGGAAATCGTGGCGATTATATTAATGATGATACATCTCCACAAAATCGTAGTTTCAGTTTTGATTTGCTCAATTCGGGTTTCAGAACTGATAGATTCTTTGAATCAGATCACATGTTCCTGGGATATGTGTCGTGGTCTGAATGTCGCCTGATTGAAATAGGAAAACTGGTCAATGAAGCCTACACTGAGGCCACATTTCAGATTGTAGCCTACAATGAATTCAAAACAGAACAGCTCCGCATCACAAGCTGCGGAGTTCGTTTTGTATATGCTAATCCAGATGAAATGCTGGATCTAAACATGCCGCCGCCCCAAGTTCATGTGGACAGTTGTGAGACTGGAGTTTGTTTTATGGACGACCCATATGAATTCACAAACAAAGAAACAGAGAGGGTAGAGATTTCGAGGGAGATAATGTCATCGGAGACAGCGGAAGGGATAGACACCTGCCACACACTGCAACTGTTTCCGGAACAACAGATAGAGAGGAAAGGAGAGAAAGAGATAATGAAGATGACAGAGCAGGAGGATGGGGAGGGTTTCCGGGTGTTAAGTCCTGTATGCTTCTGCTTCTCATTGTCTGGGTGCACCATGTGTTCGAGAAAGAGAGAAACAGAAAGAGAGGGAGACAGAGTCACCAGAGTCTCTGAAGATGGTGAGTTGAGCAGCCAGAGTGGTCTGGTGGTGCCAAATTCAGAGACTGAGAGAGAAGAAGAGAGAGTTGAGGGAGGGTTCAATAATGGGAGAGGAGTCATCAACTGGAAGAGGATTGCTAGGGTCACTATGCAGCTGCTTTTATTGGCTGGGTGCAGTATCGCGTTGAAACAGACGACTTTGGTTCGCATGAAAGGAAAAAAGAGCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1515

Amino Acids

171.78

Weight (kDa)

5.4

Isoelectric Point (pI)

44.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 15 - 192 3.3e-52 TIR domain
TIR_2 PF13676 17 - 118 2.4e-14 TIR domain
NB-ARC PF00931 196 - 363 3.2e-22 NB-ARC domain
WHD_ROQ1 PF23282 432 - 499 6.5e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 707 - 816 7.7e-08 Leucine-rich repeat region
LRR_13 PF23286 716 - 800 3.2e-13 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 766 - 868 1.3e-08 Leucine-rich repeat region
LRR_13 PF23286 786 - 874 8.9e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 856 - 959 4.1e-09 Leucine-rich repeat region
LRR_13 PF23286 880 - 963 3.4e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 930 - 1003 3.8e-12 Leucine-rich repeat region
C-JID PF20160 1166 - 1320 5.1e-20 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 4136
Acc16I TGCGCA 1 cut(s) 2182
Acc36I ACCTGC 2 cut(s) 3477, 4136
Acc65I GGTACC 1 cut(s) 2652
AccB1I GGYRCC 3 cut(s) 2652, 2883, 4366
AccB7I CCANNNNNTGG 1 cut(s) 1760
AccII CGCG 2 cut(s) 132, 4499
AccIII TCCGGA 4 cut(s) 2410, 2551, 2692, 4150
AcoI YGGCCR 2 cut(s) 94, 3062
AcsI RAATTY 5 cut(s) 1248, 2825, 3899, 4049, 4372
AcuI CTGAAG 6 cut(s) 168, 602, 2351, 3386, 3464, 4350
AdeI CACNNNGTG 2 cut(s) 252, 4275
AfaI GTAC 6 cut(s) 1653, 2025, 2654, 3006, 3411, 3673
AfiI CCNNNNNNNGG 3 cut(s) 1391, 1760, 3406
AflII CTTAAG 4 cut(s) 1841, 2120, 2330, 2612
AflIII ACRYGT 2 cut(s) 3106, 3792
AjnI CCWGG 5 cut(s) 276, 1510, 3499, 3624, 3798
AjuI GAANNNNNNNTTGG 2 cut(s) 918, 950
AloI GAACNNNNNNTCC 2 cut(s) 1386, 1418
Alw21I GWGCWC 5 cut(s) 112, 156, 267, 1420, 4271
Alw44I GTGCAC 1 cut(s) 4267
AlwNI CAGNNNCTG 3 cut(s) 2562, 2844, 4382
Ama87I CYCGRG 1 cut(s) 1777
Aor13HI TCCGGA 4 cut(s) 2410, 2551, 2692, 4150
AoxI GGCC 4 cut(s) 94, 2004, 3062, 3870
ApaLI GTGCAC 1 cut(s) 4267
ApoI RAATTY 5 cut(s) 1248, 2825, 3899, 4049, 4372
ArsI GACNNNNNNTTYG 2 cut(s) 2526, 2558
AseI ATTAAT 1 cut(s) 3702
Asp700I GAANNNNTTC 6 cut(s) 580, 2085, 2196, 2406, 2688, 2829
Asp718I GGTACC 1 cut(s) 2652
AspLEI GCGC 3 cut(s) 134, 1521, 2183
AspS9I GGNCC 2 cut(s) 432, 2938
AsuC2I CCSGG 2 cut(s) 1868, 4226
AsuHPI GGTGA 5 cut(s) 430, 634, 1016, 4309, 4349
AvaI CYCGRG 1 cut(s) 1777
AvaII GGWCC 2 cut(s) 432, 2938
AxyI CCTNAGG 1 cut(s) 3360
BaeGI GKGCMC 3 cut(s) 2164, 2888, 4271
BalI TGGCCA 1 cut(s) 3064
BamHI GGATCC 1 cut(s) 331
BanI GGYRCC 3 cut(s) 2652, 2883, 4366
BanII GRGCYC 3 cut(s) 112, 156, 267
BarI GAAGNNNNNNTAC 2 cut(s) 35, 67
BbsI GAAGAC 3 cut(s) 43, 78, 608
Bbv12I GWGCWC 5 cut(s) 112, 156, 267, 1420, 4271
BceAI ACGGC 2 cut(s) 508, 2050
BcgI CGANNNNNNTGC 4 cut(s) 70, 104, 686, 720
BciT130I CCWGG 5 cut(s) 278, 1512, 3501, 3626, 3800
BclI TGATCA 2 cut(s) 1108, 2773
BcnI CCSGG 2 cut(s) 1868, 4226
BfmI CTRYAG 1 cut(s) 2253
BfrI CTTAAG 4 cut(s) 1841, 2120, 2330, 2612
BfuAI ACCTGC 2 cut(s) 3477, 4136
BglII AGATCT 1 cut(s) 84
BmcAI AGTACT 1 cut(s) 2025
Bme1390I CCNGG 7 cut(s) 278, 1512, 1868, 3501, 3626, 3800, 4226
Bme18I GGWCC 2 cut(s) 432, 2938
BmeT110I CYCGRG 1 cut(s) 1777
BmgT120I GGNCC 2 cut(s) 432, 2938
BmiI GGNNCC 5 cut(s) 333, 1645, 2654, 2885, 4368
BmrFI CCNGG 7 cut(s) 278, 1512, 1868, 3501, 3626, 3800, 4226
BmrI ACTGGG 3 cut(s) 1748, 2301, 2882
BmsI GCATC 5 cut(s) 1570, 1823, 3253, 3304, 3930
BmuI ACTGGG 3 cut(s) 1748, 2301, 2882
BpiI GAAGAC 3 cut(s) 43, 78, 608
BpmI CTGGAG 3 cut(s) 448, 1662, 4041
BpuEI CTTGAG 6 cut(s) 1076, 1102, 2036, 2377, 2519, 2801
BpuMI CCSGG 2 cut(s) 1868, 4226
Bsa29I ATCGAT 1 cut(s) 141
BsaAI YACGTR 1 cut(s) 3008
BsaBI GATNNNNATC 1 cut(s) 330
BsaI GGTCTC 1 cut(s) 2735
BsaJI CCNNGG 4 cut(s) 1511, 3500, 3625, 3799
BsaWI WCCGGW 4 cut(s) 2410, 2551, 2692, 4150
BsaXI ACNNNNNCTCC 6 cut(s) 1635, 1665, 4082, 4112, 4299, 4329
Bsc4I CCNNNNNNNGG 3 cut(s) 1391, 1760, 3406
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 7 cut(s) 1645, 1754, 2296, 2888, 3852, 4024, 4445
Bse21I CCTNAGG 1 cut(s) 3360
Bse3DI GCAATG 3 cut(s) 678, 1396, 3225
Bse8I GATNNNNATC 1 cut(s) 330
BseAI TCCGGA 4 cut(s) 2410, 2551, 2692, 4150
BseBI CCWGG 5 cut(s) 278, 1512, 3501, 3626, 3800
BseCI ATCGAT 1 cut(s) 141
BseDI CCNNGG 4 cut(s) 1511, 3500, 3625, 3799
BseGI GGATG 9 cut(s) 23, 493, 1261, 1585, 1838, 2063, 3035, 3151, 4216
BseJI GATNNNNATC 1 cut(s) 330
BseLI CCNNNNNNNGG 3 cut(s) 1391, 1760, 3406
BseMI GCAATG 3 cut(s) 678, 1396, 3225
BseMII CTCAG 8 cut(s) 70, 1015, 1629, 1992, 2898, 3374, 3858, 4374
BseNI ACTGG 7 cut(s) 1645, 1754, 2296, 2888, 3852, 4024, 4445
BseRI GAGGAG 2 cut(s) 81, 4443
BseSI GKGCMC 3 cut(s) 2164, 2888, 4271
BseYI CCCAGC 3 cut(s) 465, 868, 4484
BsgI GTGCAG 2 cut(s) 3578, 4510
Bsh1236I CGCG 2 cut(s) 132, 4499
BshFI GGCC 4 cut(s) 96, 2006, 3064, 3872
BshNI GGYRCC 3 cut(s) 2652, 2883, 4366
BshVI ATCGAT 1 cut(s) 141
BsiHKAI GWGCWC 5 cut(s) 112, 156, 267, 1420, 4271
BsiHKCI CYCGRG 1 cut(s) 1777
BsiSI CCGG 8 cut(s) 27, 93, 1868, 2411, 2552, 2693, 4151, 4225
BslFI GGGAC 7 cut(s) 1313, 1799, 2263, 2487, 2822, 2933, 3642
BslI CCNNNNNNNGG 3 cut(s) 1391, 1760, 3406
BsmFI GGGAC 7 cut(s) 1313, 1799, 2263, 2487, 2822, 2933, 3642
BsmI GAATGC 3 cut(s) 1595, 2139, 2349
BsnI GGCC 4 cut(s) 96, 2006, 3064, 3872
Bso31I GGTCTC 1 cut(s) 2735
BsoBI CYCGRG 1 cut(s) 1777
Bsp1286I GDGCHC 7 cut(s) 112, 156, 267, 1420, 2164, 2888, 4271
Bsp13I TCCGGA 4 cut(s) 2410, 2551, 2692, 4150
BspANI GGCC 4 cut(s) 96, 2006, 3064, 3872
BspCNI CTCAG 8 cut(s) 69, 1014, 1628, 1993, 2899, 3373, 3859, 4375
BspDI ATCGAT 1 cut(s) 141
BspEI TCCGGA 4 cut(s) 2410, 2551, 2692, 4150
BspFNI CGCG 2 cut(s) 132, 4499
BspHI TCATGA 2 cut(s) 2427, 2709
BspLI GGNNCC 5 cut(s) 333, 1645, 2654, 2885, 4368
BspMAI CTGCAG 1 cut(s) 2257
BspMI ACCTGC 2 cut(s) 3477, 4136
BspQI GCTCTTC 2 cut(s) 144, 416
BspT107I GGYRCC 3 cut(s) 2652, 2883, 4366
BspTI CTTAAG 4 cut(s) 1841, 2120, 2330, 2612
BspTNI GGTCTC 1 cut(s) 2735
BsrDI GCAATG 3 cut(s) 678, 1396, 3225
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 7 cut(s) 1645, 1754, 2296, 2888, 3852, 4024, 4445
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 4 cut(s) 1511, 3500, 3625, 3799
Bst2UI CCWGG 5 cut(s) 278, 1512, 3501, 3626, 3800
Bst4CI ACNGT 9 cut(s) 913, 1370, 1970, 2443, 2558, 2840, 3584, 4010, 4146
Bst6I CTCTTC 9 cut(s) 144, 408, 416, 2553, 2652, 3483, 3501, 4389, 4439
BstAFI CTTAAG 4 cut(s) 1841, 2120, 2330, 2612
BstAPI GCANNNNNTGC 1 cut(s) 307
BstBAI YACGTR 1 cut(s) 3008
BstC8I GCNNGC 9 cut(s) 94, 497, 1125, 1838, 1896, 2522, 2574, 3049, 3262
BstF5I GGATG 9 cut(s) 23, 493, 1261, 1585, 1838, 2063, 3035, 3151, 4216
BstFNI CGCG 2 cut(s) 132, 4499
BstHHI GCGC 3 cut(s) 134, 1521, 2183
BstMWI GCNNNNNNNGC 8 cut(s) 131, 307, 1580, 1891, 2443, 2725, 3608, 3927
BstNI CCWGG 5 cut(s) 278, 1512, 3501, 3626, 3800
BstNSI RCATGY 9 cut(s) 950, 1127, 1323, 1453, 1894, 2524, 3110, 3796, 3985
BstSCI CCNGG 7 cut(s) 276, 1510, 1866, 3499, 3624, 3798, 4224
BstSFI CTRYAG 1 cut(s) 2253
BstSLI GKGCMC 3 cut(s) 2164, 2888, 4271
BstUI CGCG 2 cut(s) 132, 4499
BstV2I GAAGAC 3 cut(s) 43, 78, 608
BstX2I RGATCY 8 cut(s) 84, 331, 454, 2098, 2308, 2590, 2872, 3973
BstXI CCANNNNNNTGG 1 cut(s) 3573
BstYI RGATCY 8 cut(s) 84, 331, 454, 2098, 2308, 2590, 2872, 3973
Bsu15I ATCGAT 1 cut(s) 141
Bsu36I CCTNAGG 1 cut(s) 3360
BsuRI GGCC 4 cut(s) 96, 2006, 3064, 3872
BsuTUI ATCGAT 1 cut(s) 141
BtsCI GGATG 9 cut(s) 23, 493, 1261, 1585, 1838, 2063, 3035, 3151, 4216
BtsI GCAGTG 2 cut(s) 1218, 4136
BtsIMutI CAGTG 7 cut(s) 1218, 1966, 2289, 2448, 3555, 3864, 4136
BveI ACCTGC 2 cut(s) 3477, 4136
Cac8I GCNNGC 9 cut(s) 94, 497, 1125, 1838, 1896, 2522, 2574, 3049, 3262
CaiI CAGNNNCTG 3 cut(s) 2562, 2844, 4382
CciI TCATGA 2 cut(s) 2427, 2709
CfoI GCGC 3 cut(s) 134, 1521, 2183
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 2 cut(s) 432, 2938
ClaI ATCGAT 1 cut(s) 141
Csp6I GTAC 6 cut(s) 1652, 2024, 2653, 3005, 3410, 3672
CviQI GTAC 6 cut(s) 1652, 2024, 2653, 3005, 3410, 3672
DraI TTTAAA 1 cut(s) 757
DraIII CACNNNGTG 2 cut(s) 252, 4275
EaeI YGGCCR 2 cut(s) 94, 3062
Eam1104I CTCTTC 9 cut(s) 144, 408, 416, 2553, 2652, 3483, 3501, 4389, 4439
EarI CTCTTC 9 cut(s) 144, 408, 416, 2553, 2652, 3483, 3501, 4389, 4439
EciI GGCGGA 1 cut(s) 1478
Ecl136II GAGCTC 3 cut(s) 110, 154, 265
Eco24I GRGCYC 3 cut(s) 112, 156, 267
Eco31I GGTCTC 1 cut(s) 2735
Eco47I GGWCC 2 cut(s) 432, 2938
Eco53kI GAGCTC 3 cut(s) 110, 154, 265
Eco57I CTGAAG 6 cut(s) 168, 602, 2351, 3386, 3464, 4350
Eco81I CCTNAGG 1 cut(s) 3360
Eco88I CYCGRG 1 cut(s) 1777
EcoICRI GAGCTC 3 cut(s) 110, 154, 265
EcoO109I RGGNCCY 2 cut(s) 432, 2938
EcoRI GAATTC 2 cut(s) 3899, 4049
EcoRII CCWGG 5 cut(s) 276, 1510, 3499, 3624, 3798
EcoT22I ATGCAT 4 cut(s) 241, 952, 1455, 1634
EcoT38I GRGCYC 3 cut(s) 112, 156, 267
FalI AAGNNNNNCTT 2 cut(s) 1038, 1070
FaqI GGGAC 7 cut(s) 1313, 1799, 2263, 2487, 2822, 2933, 3642
FauI CCCGC 4 cut(s) 86, 1394, 1845, 2799
FauNDI CATATG 5 cut(s) 1823, 2145, 2355, 2637, 4045
FbaI TGATCA 2 cut(s) 1108, 2773
FokI GGATG 9 cut(s) 10, 500, 1268, 1592, 1845, 2050, 3042, 3158, 4223
FriOI GRGCYC 3 cut(s) 112, 156, 267
FspI TGCGCA 1 cut(s) 2182
GlaI GCGC 3 cut(s) 133, 1520, 2182
GsaI CCCAGC 3 cut(s) 469, 872, 4488
GsuI CTGGAG 3 cut(s) 448, 1662, 4041
HaeIII GGCC 4 cut(s) 96, 2006, 3064, 3872
HapII CCGG 8 cut(s) 27, 93, 1868, 2411, 2552, 2693, 4151, 4225
HhaI GCGC 3 cut(s) 134, 1521, 2183
Hin6I GCGC 3 cut(s) 132, 1519, 2181
HinP1I GCGC 3 cut(s) 132, 1519, 2181
HincII GTYRAC 3 cut(s) 1411, 1447, 1624
HindII GTYRAC 3 cut(s) 1411, 1447, 1624
HindIII AAGCTT 5 cut(s) 372, 497, 750, 2999, 3258
HpaII CCGG 8 cut(s) 27, 93, 1868, 2411, 2552, 2693, 4151, 4225
HphI GGTGA 5 cut(s) 430, 634, 1016, 4309, 4349
Hpy166II GTNNAC 7 cut(s) 941, 1411, 1447, 1624, 1769, 4006, 4269
Hpy8I GTNNAC 7 cut(s) 941, 1411, 1447, 1624, 1769, 4006, 4269
HpyCH4III ACNGT 9 cut(s) 913, 1370, 1970, 2443, 2558, 2840, 3584, 4010, 4146
HpyCH4IV ACGT 3 cut(s) 2946, 2989, 3007
HpyF10VI GCNNNNNNNGC 8 cut(s) 131, 307, 1580, 1891, 2443, 2725, 3608, 3927
HpySE526I ACGT 3 cut(s) 2946, 2989, 3007
HspAI GCGC 3 cut(s) 132, 1519, 2181
Kpn2I TCCGGA 4 cut(s) 2410, 2551, 2692, 4150
KpnI GGTACC 1 cut(s) 2656
KroI GCCGGC 1 cut(s) 92
KroNI GCCGGC 1 cut(s) 94
Ksp22I TGATCA 2 cut(s) 1108, 2773
LguI GCTCTTC 2 cut(s) 144, 416
LmnI GCTCC 4 cut(s) 1643, 2434, 2716, 3921
LweI GCATC 5 cut(s) 1570, 1823, 3253, 3304, 3930
MaeII ACGT 3 cut(s) 2946, 2989, 3007
MfeI CAATTG 2 cut(s) 2487, 2769
MflI RGATCY 8 cut(s) 84, 331, 454, 2098, 2308, 2590, 2872, 3973
MhlI GDGCHC 7 cut(s) 112, 156, 267, 1420, 2164, 2888, 4271
MlsI TGGCCA 1 cut(s) 3064
MluNI TGGCCA 1 cut(s) 3064
MlyI GAGTC 7 cut(s) 978, 1452, 2329, 2611, 4323, 4332, 4440
MmeI TCCRAC 5 cut(s) 553, 622, 1197, 1258, 3114
Mox20I TGGCCA 1 cut(s) 3064
Mph1103I ATGCAT 4 cut(s) 241, 952, 1455, 1634
MroI TCCGGA 4 cut(s) 2410, 2551, 2692, 4150
MroNI GCCGGC 1 cut(s) 92
MroXI GAANNNNTTC 6 cut(s) 580, 2085, 2196, 2406, 2688, 2829
MscI TGGCCA 1 cut(s) 3064
MslI CAYNNNNRTG 2 cut(s) 2144, 2354
Msp20I TGGCCA 1 cut(s) 3064
MspA1I CMGCKG 3 cut(s) 3406, 4112, 4471
MspCI CTTAAG 4 cut(s) 1841, 2120, 2330, 2612
MspI CCGG 8 cut(s) 27, 93, 1868, 2411, 2552, 2693, 4151, 4225
MspR9I CCNGG 7 cut(s) 278, 1512, 1868, 3501, 3626, 3800, 4226
MunI CAATTG 2 cut(s) 2487, 2769
Mva1269I GAATGC 3 cut(s) 1595, 2139, 2349
MvaI CCWGG 5 cut(s) 278, 1512, 3501, 3626, 3800
MvnI CGCG 2 cut(s) 132, 4499
MwoI GCNNNNNNNGC 8 cut(s) 131, 307, 1580, 1891, 2443, 2725, 3608, 3927
NaeI GCCGGC 1 cut(s) 94
NciI CCSGG 2 cut(s) 1868, 4226
NdeI CATATG 5 cut(s) 1823, 2145, 2355, 2637, 4045
NgoMIV GCCGGC 1 cut(s) 92
NlaIV GGNNCC 5 cut(s) 333, 1645, 2654, 2885, 4368
NmeAIII GCCGAG 1 cut(s) 688
NmuCI GTSAC 7 cut(s) 976, 1901, 1964, 3008, 3119, 4315, 4459
NsbI TGCGCA 1 cut(s) 2182
NsiI ATGCAT 4 cut(s) 241, 952, 1455, 1634
NspI RCATGY 9 cut(s) 950, 1127, 1323, 1453, 1894, 2524, 3110, 3796, 3985
PaeI GCATGC 2 cut(s) 1127, 2524
PaeR7I CTCGAG 1 cut(s) 1777
PagI TCATGA 2 cut(s) 2427, 2709
PaqCI CACCTGC 1 cut(s) 4136
PciI ACATGT 2 cut(s) 3106, 3792
PciSI GCTCTTC 2 cut(s) 144, 416
PctI GAATGC 3 cut(s) 1595, 2139, 2349
PdiI GCCGGC 1 cut(s) 94
PdmI GAANNNNTTC 6 cut(s) 580, 2085, 2196, 2406, 2688, 2829
PflFI GACNNNGTC 2 cut(s) 1442, 4313
PflMI CCANNNNNTGG 1 cut(s) 1760
PfoI TCCNGGA 2 cut(s) 276, 1866
PleI GAGTC 7 cut(s) 977, 1451, 2329, 2611, 4322, 4331, 4439
PpsI GAGTC 7 cut(s) 977, 1451, 2329, 2611, 4322, 4331, 4439
Ppu21I YACGTR 1 cut(s) 3008
PpuMI RGGWCCY 2 cut(s) 432, 2938
PscI ACATGT 2 cut(s) 3106, 3792
PshBI ATTAAT 1 cut(s) 3702
Psp124BI GAGCTC 3 cut(s) 112, 156, 267
Psp5II RGGWCCY 2 cut(s) 432, 2938
Psp6I CCWGG 5 cut(s) 276, 1510, 3499, 3624, 3798
PspFI CCCAGC 3 cut(s) 465, 868, 4484
PspGI CCWGG 5 cut(s) 276, 1510, 3499, 3624, 3798
PspN4I GGNNCC 5 cut(s) 333, 1645, 2654, 2885, 4368
PspPI GGNCC 2 cut(s) 432, 2938
PspPPI RGGWCCY 2 cut(s) 432, 2938
PstI CTGCAG 1 cut(s) 2257
PstNI CAGNNNCTG 3 cut(s) 2562, 2844, 4382
PsuI RGATCY 8 cut(s) 84, 331, 454, 2098, 2308, 2590, 2872, 3973
PsyI GACNNNGTC 2 cut(s) 1442, 4313
PvuII CAGCTG 2 cut(s) 3406, 4471
RsaI GTAC 6 cut(s) 1653, 2025, 2654, 3006, 3411, 3673
RsaNI GTAC 6 cut(s) 1652, 2024, 2653, 3005, 3410, 3672
RseI CAYNNNNRTG 2 cut(s) 2144, 2354
SacI GAGCTC 3 cut(s) 112, 156, 267
SapI GCTCTTC 2 cut(s) 144, 416
Sau96I GGNCC 2 cut(s) 432, 2938
ScaI AGTACT 1 cut(s) 2025
SchI GAGTC 7 cut(s) 978, 1452, 2329, 2611, 4323, 4332, 4440
ScrFI CCNGG 7 cut(s) 278, 1512, 1868, 3501, 3626, 3800, 4226
SduI GDGCHC 7 cut(s) 112, 156, 267, 1420, 2164, 2888, 4271
SfaNI GCATC 5 cut(s) 1570, 1823, 3253, 3304, 3930
SfcI CTRYAG 1 cut(s) 2253
Sfr274I CTCGAG 1 cut(s) 1777
SinI GGWCC 2 cut(s) 432, 2938
SlaI CTCGAG 1 cut(s) 1777
SmiMI CAYNNNNRTG 2 cut(s) 2144, 2354
SphI GCATGC 2 cut(s) 1127, 2524
SspI AATATT 2 cut(s) 172, 1136
SstI GAGCTC 3 cut(s) 112, 156, 267
StyD4I CCNGG 7 cut(s) 276, 1510, 1866, 3499, 3624, 3798, 4224
TaaI ACNGT 9 cut(s) 913, 1370, 1970, 2443, 2558, 2840, 3584, 4010, 4146
TaiI ACGT 3 cut(s) 2949, 2992, 3010
TatI WGTACW 1 cut(s) 2023
TauI GCSGC 2 cut(s) 3988, 3991
TscAI CASTG 7 cut(s) 1218, 1973, 2296, 2448, 3555, 3871, 4143
TseFI GTSAC 7 cut(s) 976, 1901, 1964, 3008, 3119, 4315, 4459
Tsp45I GTSAC 7 cut(s) 976, 1901, 1964, 3008, 3119, 4315, 4459
TspGWI ACGGA 3 cut(s) 361, 1592, 3027
TspRI CASTG 7 cut(s) 1218, 1973, 2296, 2448, 3555, 3871, 4143
Tth111I GACNNNGTC 2 cut(s) 1442, 4313
Van91I CCANNNNNTGG 1 cut(s) 1760
Vha464I CTTAAG 4 cut(s) 1841, 2120, 2330, 2612
VneI GTGCAC 1 cut(s) 4267
VpaK11BI GGWCC 2 cut(s) 432, 2938
VspI ATTAAT 1 cut(s) 3702
XapI RAATTY 5 cut(s) 1248, 2825, 3899, 4049, 4372
XbaI TCTAGA 2 cut(s) 1309, 2242
XceI RCATGY 9 cut(s) 950, 1127, 1323, 1453, 1894, 2524, 3110, 3796, 3985
XhoI CTCGAG 1 cut(s) 1777
XmnI GAANNNNTTC 6 cut(s) 580, 2085, 2196, 2406, 2688, 2829
ZrmI AGTACT 1 cut(s) 2025
Zsp2I ATGCAT 4 cut(s) 241, 952, 1455, 1634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.