RchiOBHm_Chr1g0372491

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000067
Physical Location & Seq
Forward (+)
61140649 .. 61152849
12201 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59647

Sequence Viewer

Length: 4068 bp
ATGGCTGCTTCTTCATCTCTCTCATCTGGTGGCCGTTGGAACTATGATGTGTTCCTCAGTTTTAGAGGTCAGGACACTCGCAAGATCTTTGTAGGCCATCTCCACAAAGCTCTTGATCTGAAAGCAATCAACGCATTCATAGACTCTGAAGATCTCTGCAAAGGCAACGACCTTTCAGAATTGCTCAGGGCTATTGAAGACTCTAGGCTTTCTATCGTAGTTTTCTCCCAAAACTATGCTTCTTCCACATGGTGCTTGAAAGAACTGGTTAAGATACTTGAATGCGTGGCAGCGAAGAAGCAGGTAGTGGTGCCCATTTTCTATGAAGTAGATCCATCTGATATCCGCAAACTGAAGGGGAGCTTTGCCGAGGCTTTTGCGAGACATGAGCAGGATTATAATGCCAACATGGAAGAGGTGAATAGGTGGAAGATTGCTCTAACTGAAGCCACCAATTTATCTGGTTGGGATTCACGAAATTACAAGGATGATGCTAAGTTTATTGAAAAAATTGTAGAAGATATTTCCGAAAAATTTGTCCACATCTCCTCCAATAAAGTCAATGACTTGGTTGGAATGGATTCCCACGTAGAAAAAATGTGTTCACTATTATGTCTTGGTGGGGATGATGTTCGTGTTGTTGGAGTATGCGGAATGCCTGGTATAGGAAAAACAACTATTGCCAAAGCTGTTTATGACAAACTCCTTTGTCAATTTGAACACTATTGCTTTCTTGAAAATGTCAAGGACGGTTTCAAGAACAATGGTGCAATACACATGCAGGAAGAACTCCTATCTAGAATATTTGAGAAAAGAGTGCGCTGTCTAGGCACTTTGAGTAAAGGATCCAAGATGATAATGGAAAGGCTGAGTAAGAAAAAAGTTCTGCTTGTTCTTGATGATGTGGAAAACTTTGCGCAAATTGAGGCGTTACTTGGAAACCAGCATTCATTTGGTGGTGGAAGTAGAATCATTGTAACCACTAGAGATATACAATCACTCAGTGGAGTTGATGCGAGATATAGTCCCATGTTTCTAAGTGATGATGAAGCTCTTGAGCTCTTTATGCAGTATGCCTTCAGAACAAACCAACCTACAAGAGAGTATGATCCTCTCTCAAGGCGTGCCGTAGAATATGCTCAAGGTCTGCCTCTAGCACTCAAAGTTTTGGGAGCTTTTCTTGATGACAAAAGTATATGTGAGTGGGAAGATGAGCTAGAGAAAATAAAGGAAATCCCACATATAGAAATTCAGGGTGTGCTTAAAAAAAGTTTTGATGGCCTGGATCCTTCACAGAAGGACATATTCCTAGATATTGCATGTTTCTTTAGAGGAATGGACAAAGGCTTTGTAACCAAAATTCTGGAAAGTTGTGGCTTCTATCCCCATAGTGGATTGCGAGTGCTAATTGATAGAGCTCTCGTAATTATTTCAGATGACAACAGGCTGGAAATGCATGATTTAATAAAGGATATGGGTTGGGAGATTATCCGCCAACAATCTATTAAAGAGCCTGGGAAGCGCAGTAGGTTGTGGGTTTATGAAGATGTTGCTCGTGTGTTAACTCAAAATATGGCTACAGATGCGGTTGAATGCATAATGCTAGACTTGTCGAAGTCAAAGGACGTCTACATAGATGCTGAAGCTTTTGTTAGAATGACGAAATTAAGACTGCTCAGAATCGGTTATAACCACTCCATAGACTTGAATGCGAAAGATTTTCAATCGAAGCAGTTACACCATCCAAGAGGCGAGTGTAAACAACATGTAAGTGGGGACGTAAAGTTTCTTTCTCATGACTTGAGGTATCTCATGTGGTATGGATGCCCCCTGAAGTCTTTGCCATCCAATTTTCACCCAAAGAATCTTGTTGACCTTGACATGCGTTGTAGCCATATTGAACATCTTTGGGCAGAAATCAAGTCCTTCAAAAAGCTGAAATTTATCAATTTGAGTCATTCTTATTACCTTACTACAACTCCCAACTTCACTGAAGCAACAAATGTGGAAACACTTGTTCTTGAAGGGTGTTCCAGTTTACTTGATGTTAACTCATCCATTTCAGCTCTTAAAAACCTTGTATTCTTGTGCCTAAGAGGATGCAAAGAACTTAAGAGTCTTCCAAGCAGCATTTGTTTGAAGTCTCTTAAAACCCTTGATCTTTCTGGCTGCTCAAGTCTCGCAAAGTTTCCAGAGATTTCAGGGATTATGGAGGACCTGTCAGAAATTTATCTAAATGATACTGCAATTGAAGAATTGCCCTCATCAATTGAACGACTTCAGGGGCTTGTGTTATTACATTTGAGAAACTGTAGAAGCCTTCTCTGTCTTCCAGACACTATATGTAATCTGGTATATCTTAAAGATCTCACTGTCTCTGGGTGCTCAAAGCTTTATCATTTGCCTGAGAACTTGGGGAATTTAGAATCCCTGATGTGCCTTGAAGTAGAAGGAAGTGGTATAAGAGAACTCCCAGTTTCTATCTTATGCTTGAAAAGGCTGAAAACATTATCATGTGACGGATGTACAGAAATGACGATGCCCTTTTCATCATGGTCATCATCAATTGAAGAATATTGTAGTTACTCTGGTCTGCTGCATCTTGATTTAAGTGACAGTAATCTGTGTGAATTATCAGATGGTATTGCTCAATTGTCCTCATTGAAAACCTTAGAGCTGCGCGGAACCAACTTGGAGAGCTTACCTGCAACTATGAATCAACTTCGCTGTTTGACGCGTCTTGAATTGGAAGCTTGCAAGAGACTGAAATCAGTACCAGAGCTTCCATCAACCATAAATTACATTGATGCTCATGACTGCAGCGCATTGGCAACCGTTGCAAAACCAAATACTCGTTGCTCTATGAATCTTTGCTTCACATTTTCTAATTGCCTCCAAATGGTACAAACGAATCTATTCAGAGATATTGTGGAAACTCATTCTTCTTACCAGGGTAATTATCAACGTCCACTATCCTTTAATATGTCTCTTCCCGGAAGTGAAATTCCTGATTGGTTCAATTATCAATGTAGGGGATCCTCACTAACTGTACAGCTACCCCCAAATTGGTTCGATAATAAGTTTTTGGGGTTTGCCATATGTGCTGTTAGCGACTTCAAGGTTTCTCATAATGATGCATCTGATCTATCCGCTCTATGTCATTGTGGCTTAAAAGGAAATCATGGTCAGTACAGTTTCAGTTTTACTTTGCTTGATTGGGGTTTCACTACTGAGAGAATCCTTGAGTCAGATCATATGTTCCTGGCATATGTGCCATGGTCTGAATATCGCTTCATTGTAGAGGGAAAACTGGTCAATGAACGGTATTTCACTGAGGCCACATTTGAGATTGTAGTAGAAAATAGAGCTGATAGCCATGTTTTCGGGACAGTAAAAAACCACCATTGCATCACAAGTTGTGGAGTTCGTTTCTTTCATAGTAGTTGCATGGAAGAGCAAAATCTAAACATCAGTGAAACCAAATCTCATCATGACAGCTGTGACAGTAGGAGAGGAAAAGAGGGGGTGGGGGTGCAGTTGGAGGTAAATCCAGCACTGGTGATACTCCCTGAGGAAGCATTCTCGAGATGTGAATGGTCTCCGAAAGCGGACCAGAGCTCAATTAGTACAAGCGAAGTTTCAATGGAGGATCAGAGGAATTCCTACAAGCGAAGAAAGAATTCAGAGTCATGGACAGGGGTTTCACCCACAGTTGAAGATGGTCAGGCTTGGAAGACATTTGGGCAGAAGGAAATCCCCAATGCTCCATATCCAAGGGAAGGTCTAGCACGGTGGTCACAACAAGTAAGAGTAACACCAGATATCAGGCTTGATGAGGGGTCTCTTGATGACGGCTTTAGCTGGAGGAAGTATGGCCAAAAGGACATCTTGGGAGCCAAATATCCGAGAGGGTATTACAGATGCACTAATCGAAATATTCAAGGTTGTTTGGCCACAAAGCAAGTGCAACGCGCCGATGAGGACCCAATGATCGTCAAAATTACTTACCAAGGAAGGCACACTTGTACTCAAGCAGCCTCCGTTACAGTAAATAGTCCTCCGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1355

Amino Acids

153.17

Weight (kDa)

5.93

Isoelectric Point (pI)

44.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 14 - 190 1.3e-54 TIR domain
TIR_2 PF13676 17 - 118 8.8e-15 TIR domain
NB-ARC PF00931 195 - 363 1e-18 NB-ARC domain
WHD_ROQ1 PF23282 432 - 501 5.3e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 713 - 798 1.3e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 780 - 845 9.9e-10 Leucine-rich repeat region
LRR_13 PF23286 800 - 953 4.9e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 855 - 922 1.2e-09 Leucine-rich repeat region
C-JID PF20160 997 - 1147 5.9e-22 C-JID domain
WRKY PF03106 1283 - 1342 2e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 399, 1689
AatII GACGTC 1 cut(s) 1629
Acc16I TGCGCA 1 cut(s) 918
Acc36I ACCTGC 2 cut(s) 292, 2713
AccB1I GGYRCC 1 cut(s) 310
AccBSI CCGCTC 1 cut(s) 3152
AccI GTMKAC 1 cut(s) 1629
AccII CGCG 3 cut(s) 2682, 2737, 3972
AciI CCGC 7 cut(s) 346, 651, 1492, 1586, 2682, 3150, 3608
AclWI GGATC 9 cut(s) 326, 840, 853, 1103, 1280, 1293, 3030, 3043, 3657
AcoI YGGCCR 3 cut(s) 31, 3874, 3951
AcsI RAATTY 9 cut(s) 533, 1248, 1359, 1940, 2226, 2419, 3003, 3658, 3679
AcuI CTGAAG 8 cut(s) 168, 374, 465, 1063, 1662, 1853, 2013, 2264
AcyI GRCGYC 1 cut(s) 1626
AdeI CACNNNGTG 2 cut(s) 252, 1004
AfaI GTAC 7 cut(s) 2527, 2775, 2904, 3051, 3191, 3628, 4027
AfiI CCNNNNNNNGG 5 cut(s) 665, 1391, 2899, 3066, 3779
AflII CTTAAG 1 cut(s) 2111
AflIII ACRYGT 2 cut(s) 1765, 2735
AjnI CCWGG 5 cut(s) 658, 1281, 1513, 2949, 3261
Alw21I GWGCWC 4 cut(s) 1062, 1420, 2387, 3620
Alw26I GTCTC 8 cut(s) 376, 2148, 2183, 2380, 2755, 2991, 3603, 3846
AlwI GGATC 9 cut(s) 326, 840, 853, 1103, 1280, 1293, 3030, 3043, 3657
Ama87I CYCGRG 1 cut(s) 3583
AoxI GGCC 7 cut(s) 31, 94, 1279, 3336, 3874, 3951, 4062
ApeKI GCWGC 8 cut(s) 5, 290, 2127, 2169, 2596, 2677, 2820, 4034
ApoI RAATTY 9 cut(s) 533, 1248, 1359, 1940, 2226, 2419, 3003, 3658, 3679
ArsI GACNNNNNNTTYG 4 cut(s) 522, 554, 1331, 1363
Asp700I GAANNNNTTC 4 cut(s) 580, 2277, 2915, 3679
AspLEI GCGC 6 cut(s) 822, 919, 1524, 2682, 2825, 3974
AspS9I GGNCC 3 cut(s) 2215, 3610, 3982
AsuC2I CCSGG 1 cut(s) 2994
AsuHPI GGTGA 4 cut(s) 430, 1847, 3571, 3696
AvaI CYCGRG 1 cut(s) 3583
AvaII GGWCC 3 cut(s) 2215, 3610, 3982
AxyI CCTNAGG 1 cut(s) 3570
BaeGI GKGCMC 1 cut(s) 315
BalI TGGCCA 2 cut(s) 3876, 3953
BamHI GGATCC 3 cut(s) 845, 1285, 3035
BanI GGYRCC 1 cut(s) 310
BanII GRGCYC 3 cut(s) 1062, 1420, 3620
BarI GAAGNNNNNNTAC 2 cut(s) 2766, 2798
BauI CACGAG 1 cut(s) 1554
BbsI GAAGAC 4 cut(s) 204, 2111, 2321, 3740
Bbv12I GWGCWC 4 cut(s) 1062, 1420, 2387, 3620
BbvI GCAGC 7 cut(s) 302, 2139, 2156, 2583, 2664, 2832, 4046
BccI CCATC 8 cut(s) 105, 343, 1271, 1749, 1852, 2633, 2794, 3713
BceAI ACGGC 3 cut(s) 18, 1112, 3868
BcgI CGANNNNNNTGC 2 cut(s) 359, 393
BciT130I CCWGG 5 cut(s) 660, 1283, 1515, 2951, 3263
BcnI CCSGG 1 cut(s) 2994
BcoDI GTCTC 8 cut(s) 376, 2148, 2183, 2380, 2755, 2991, 3603, 3846
BfmI CTRYAG 3 cut(s) 1578, 2311, 2818
BfrI CTTAAG 1 cut(s) 2111
BfuAI ACCTGC 2 cut(s) 292, 2713
BglII AGATCT 3 cut(s) 84, 151, 2365
BisI GCNGC 8 cut(s) 6, 291, 2128, 2170, 2597, 2678, 2821, 4035
BlsI GCNGC 8 cut(s) 7, 292, 2129, 2171, 2598, 2679, 2822, 4036
Bme1390I CCNGG 6 cut(s) 660, 1283, 1515, 2951, 2994, 3263
Bme18I GGWCC 3 cut(s) 2215, 3610, 3982
BmeT110I CYCGRG 1 cut(s) 3583
BmgT120I GGNCC 3 cut(s) 2215, 3610, 3982
BmiI GGNNCC 7 cut(s) 312, 847, 1287, 2686, 3037, 3895, 3984
BmrFI CCNGG 6 cut(s) 660, 1283, 1515, 2951, 2994, 3263
BmrI ACTGGG 1 cut(s) 2468
BmuI ACTGGG 1 cut(s) 2468
BpiI GAAGAC 4 cut(s) 204, 2111, 2321, 3740
BpmI CTGGAG 1 cut(s) 3883
Bpu10I CCTNAGC 1 cut(s) 185
BpuEI CTTGAG 7 cut(s) 1076, 1102, 1125, 1822, 2158, 3263, 4014
BpuMI CCSGG 1 cut(s) 2994
BsaAI YACGTR 1 cut(s) 589
BsaHI GRCGYC 1 cut(s) 1626
BsaI GGTCTC 2 cut(s) 3603, 3846
BsaJI CCNNGG 6 cut(s) 369, 1514, 2950, 3275, 3773, 4009
BsaXI ACNNNNNCTCC 6 cut(s) 533, 563, 1963, 1993, 2204, 2234
Bsc4I CCNNNNNNNGG 5 cut(s) 665, 1391, 2899, 3066, 3779
Bse1I ACTGG 5 cut(s) 270, 2034, 2474, 3315, 3561
Bse21I CCTNAGG 1 cut(s) 3570
Bse3DI GCAATG 1 cut(s) 3403
BseBI CCWGG 5 cut(s) 660, 1283, 1515, 2951, 3263
BseDI CCNNGG 6 cut(s) 369, 1514, 2950, 3275, 3773, 4009
BseGI GGATG 8 cut(s) 493, 631, 1741, 1829, 1844, 2054, 2105, 2528
BseLI CCNNNNNNNGG 5 cut(s) 665, 1391, 2899, 3066, 3779
BseMI GCAATG 1 cut(s) 3403
BseMII CTCAG 9 cut(s) 70, 199, 860, 1015, 1690, 2397, 3222, 3324, 3561
BseNI ACTGG 5 cut(s) 270, 2034, 2474, 3315, 3561
BseRI GAGGAG 1 cut(s) 538
BseSI GKGCMC 1 cut(s) 315
BseXI GCAGC 7 cut(s) 302, 2139, 2156, 2583, 2664, 2832, 4046
BsgI GTGCAG 1 cut(s) 3554
Bsh1236I CGCG 3 cut(s) 2682, 2737, 3972
BshFI GGCC 7 cut(s) 33, 96, 1281, 3338, 3876, 3953, 4064
BshNI GGYRCC 1 cut(s) 310
BsiHKAI GWGCWC 4 cut(s) 1062, 1420, 2387, 3620
BsiHKCI CYCGRG 1 cut(s) 3583
BsiSI CCGG 2 cut(s) 2994, 4061
BslFI GGGAC 3 cut(s) 1011, 1790, 3400
BslI CCNNNNNNNGG 5 cut(s) 665, 1391, 2899, 3066, 3779
BsmAI GTCTC 8 cut(s) 376, 2148, 2183, 2380, 2755, 2991, 3603, 3846
BsmFI GGGAC 3 cut(s) 1011, 1790, 3400
BsmI GAATGC 7 cut(s) 134, 287, 660, 946, 1598, 1714, 3578
BsnI GGCC 7 cut(s) 33, 96, 1281, 3338, 3876, 3953, 4064
Bso31I GGTCTC 2 cut(s) 3603, 3846
BsoBI CYCGRG 1 cut(s) 3583
Bsp1286I GDGCHC 5 cut(s) 315, 1062, 1420, 2387, 3620
Bsp1407I TGTACA 2 cut(s) 2525, 3049
Bsp19I CCATGG 1 cut(s) 3275
BspACI CCGC 7 cut(s) 346, 651, 1492, 1586, 2682, 3150, 3608
BspANI GGCC 7 cut(s) 33, 96, 1281, 3338, 3876, 3953, 4064
BspCNI CTCAG 9 cut(s) 69, 198, 861, 1014, 1689, 2398, 3223, 3325, 3562
BspFNI CGCG 3 cut(s) 2682, 2737, 3972
BspHI TCATGA 3 cut(s) 1795, 2812, 3490
BspLI GGNNCC 7 cut(s) 312, 847, 1287, 2686, 3037, 3895, 3984
BspMAI CTGCAG 1 cut(s) 2822
BspMI ACCTGC 2 cut(s) 292, 2713
BspPI GGATC 9 cut(s) 326, 840, 853, 1103, 1280, 1293, 3030, 3043, 3657
BspQI GCTCTTC 1 cut(s) 3447
BspT107I GGYRCC 1 cut(s) 310
BspTI CTTAAG 1 cut(s) 2111
BspTNI GGTCTC 2 cut(s) 3603, 3846
BsrBI CCGCTC 1 cut(s) 3152
BsrDI GCAATG 1 cut(s) 3403
BsrGI TGTACA 2 cut(s) 2525, 3049
BsrI ACTGG 5 cut(s) 270, 2034, 2474, 3315, 3561
BssECI CCNNGG 6 cut(s) 369, 1514, 2950, 3275, 3773, 4009
BssNI GRCGYC 1 cut(s) 1626
BssSI CACGAG 1 cut(s) 1554
BssT1I CCWWGG 3 cut(s) 3275, 3773, 4009
Bst2BI CACGAG 1 cut(s) 1554
Bst2UI CCWGG 5 cut(s) 660, 1283, 1515, 2951, 3263
Bst6I CTCTTC 3 cut(s) 408, 2994, 3447
BstACI GRCGYC 1 cut(s) 1626
BstAFI CTTAAG 1 cut(s) 2111
BstAPI GCANNNNNTGC 1 cut(s) 2837
BstAUI TGTACA 2 cut(s) 2525, 3049
BstBAI YACGTR 1 cut(s) 589
BstC8I GCNNGC 2 cut(s) 1125, 2755
BstDSI CCRYGG 1 cut(s) 3275
BstENI CCTNNNNNAGG 1 cut(s) 663
BstF5I GGATG 8 cut(s) 493, 631, 1741, 1829, 1844, 2054, 2105, 2528
BstFNI CGCG 3 cut(s) 2682, 2737, 3972
BstHHI GCGC 6 cut(s) 822, 919, 1524, 2682, 2825, 3974
BstMAI GTCTC 8 cut(s) 376, 2148, 2183, 2380, 2755, 2991, 3603, 3846
BstMWI GCNNNNNNNGC 8 cut(s) 131, 828, 1066, 1453, 1519, 1583, 2837, 3101
BstNI CCWGG 5 cut(s) 660, 1283, 1515, 2951, 3263
BstNSI RCATGY 4 cut(s) 781, 1323, 1769, 1885
BstSCI CCNGG 6 cut(s) 658, 1281, 1513, 2949, 2992, 3261
BstSFI CTRYAG 3 cut(s) 1578, 2311, 2818
BstSLI GKGCMC 1 cut(s) 315
BstUI CGCG 3 cut(s) 2682, 2737, 3972
BstV1I GCAGC 7 cut(s) 302, 2139, 2156, 2583, 2664, 2832, 4046
BstV2I GAAGAC 4 cut(s) 204, 2111, 2321, 3740
BstX2I RGATCY 7 cut(s) 84, 151, 331, 845, 1285, 2365, 3035
BstXI CCANNNNNNTGG 1 cut(s) 1363
BstYI RGATCY 7 cut(s) 84, 151, 331, 845, 1285, 2365, 3035
Bsu36I CCTNAGG 1 cut(s) 3570
BsuRI GGCC 7 cut(s) 33, 96, 1281, 3338, 3876, 3953, 4064
BtgI CCRYGG 1 cut(s) 3275
BtsCI GGATG 8 cut(s) 493, 631, 1741, 1829, 1844, 2054, 2105, 2528
BtsIMutI CAGTG 6 cut(s) 1009, 1989, 2370, 3330, 3478, 3554
BveI ACCTGC 2 cut(s) 292, 2713
Cac8I GCNNGC 2 cut(s) 1125, 2755
CciI TCATGA 3 cut(s) 1795, 2812, 3490
CfoI GCGC 6 cut(s) 822, 919, 1524, 2682, 2825, 3974
Cfr13I GGNCC 3 cut(s) 2215, 3610, 3982
CseI GACGC 2 cut(s) 2726, 2743
Csp6I GTAC 7 cut(s) 2526, 2774, 2903, 3050, 3190, 3627, 4026
CspCI CAANNNNNGTGG 2 cut(s) 1986, 2021
CviQI GTAC 7 cut(s) 2526, 2774, 2903, 3050, 3190, 3627, 4026
DraIII CACNNNGTG 2 cut(s) 252, 1004
EaeI YGGCCR 3 cut(s) 31, 3874, 3951
Eam1104I CTCTTC 3 cut(s) 408, 2994, 3447
EarI CTCTTC 3 cut(s) 408, 2994, 3447
EciI GGCGGA 1 cut(s) 1481
Ecl136II GAGCTC 3 cut(s) 1060, 1418, 3618
Eco130I CCWWGG 3 cut(s) 3275, 3773, 4009
Eco24I GRGCYC 3 cut(s) 1062, 1420, 3620
Eco31I GGTCTC 2 cut(s) 3603, 3846
Eco32I GATATC 2 cut(s) 343, 3823
Eco47I GGWCC 3 cut(s) 2215, 3610, 3982
Eco53kI GAGCTC 3 cut(s) 1060, 1418, 3618
Eco57I CTGAAG 8 cut(s) 168, 374, 465, 1063, 1662, 1853, 2013, 2264
Eco81I CCTNAGG 1 cut(s) 3570
Eco88I CYCGRG 1 cut(s) 3583
EcoICRI GAGCTC 3 cut(s) 1060, 1418, 3618
EcoNI CCTNNNNNAGG 1 cut(s) 663
EcoO109I RGGNCCY 2 cut(s) 2215, 3982
EcoRI GAATTC 2 cut(s) 3658, 3679
EcoRII CCWGG 5 cut(s) 658, 1281, 1513, 2949, 3261
EcoRV GATATC 2 cut(s) 343, 3823
EcoT14I CCWWGG 3 cut(s) 3275, 3773, 4009
EcoT22I ATGCAT 3 cut(s) 1458, 1598, 3139
EcoT38I GRGCYC 3 cut(s) 1062, 1420, 3620
ErhI CCWWGG 3 cut(s) 3275, 3773, 4009
FalI AAGNNNNNCTT 8 cut(s) 347, 379, 873, 905, 3872, 3904, 4006, 4038
FaqI GGGAC 3 cut(s) 1011, 1790, 3400
FauNDI CATATG 3 cut(s) 3098, 3255, 3268
FblI GTMKAC 1 cut(s) 1629
Fnu4HI GCNGC 8 cut(s) 6, 291, 2128, 2170, 2597, 2678, 2821, 4035
FokI GGATG 8 cut(s) 500, 638, 1728, 1831, 1836, 2041, 2112, 2535
FriOI GRGCYC 3 cut(s) 1062, 1420, 3620
Fsp4HI GCNGC 8 cut(s) 6, 291, 2128, 2170, 2597, 2678, 2821, 4035
FspI TGCGCA 1 cut(s) 918
GlaI GCGC 6 cut(s) 821, 918, 1523, 2681, 2824, 3973
GluI GCNGC 8 cut(s) 6, 291, 2128, 2170, 2597, 2678, 2821, 4035
GsuI CTGGAG 1 cut(s) 3883
HaeIII GGCC 7 cut(s) 33, 96, 1281, 3338, 3876, 3953, 4064
HapII CCGG 2 cut(s) 2994, 4061
HgaI GACGC 2 cut(s) 2726, 2743
HhaI GCGC 6 cut(s) 822, 919, 1524, 2682, 2825, 3974
Hin1I GRCGYC 1 cut(s) 1626
Hin6I GCGC 6 cut(s) 820, 917, 1522, 2680, 2823, 3972
HinP1I GCGC 6 cut(s) 820, 917, 1522, 2680, 2823, 3972
HincII GTYRAC 3 cut(s) 1563, 1873, 2050
HindII GTYRAC 3 cut(s) 1563, 1873, 2050
HindIII AAGCTT 3 cut(s) 1644, 2390, 2751
HpaI GTTAAC 2 cut(s) 1563, 2050
HpaII CCGG 2 cut(s) 2994, 4061
HphI GGTGA 4 cut(s) 430, 1847, 3571, 3696
Hpy166II GTNNAC 9 cut(s) 541, 605, 1563, 1630, 1760, 1873, 2039, 2050, 2969
Hpy8I GTNNAC 9 cut(s) 541, 605, 1563, 1630, 1760, 1873, 2039, 2050, 2969
HpyCH4IV ACGT 4 cut(s) 588, 1626, 1779, 2965
HpyF10VI GCNNNNNNNGC 8 cut(s) 131, 828, 1066, 1453, 1519, 1583, 2837, 3101
HpySE526I ACGT 4 cut(s) 588, 1626, 1779, 2965
Hsp92I GRCGYC 1 cut(s) 1626
HspAI GCGC 6 cut(s) 820, 917, 1522, 2680, 2823, 3972
KspAI GTTAAC 2 cut(s) 1563, 2050
LguI GCTCTTC 1 cut(s) 3447
LmnI GCTCC 4 cut(s) 360, 1172, 3769, 3893
Lsp1109I GCAGC 7 cut(s) 302, 2139, 2156, 2583, 2664, 2832, 4046
MaeII ACGT 4 cut(s) 588, 1626, 1779, 2965
MbiI CCGCTC 1 cut(s) 3152
MfeI CAATTG 4 cut(s) 2247, 2268, 2565, 2651
MflI RGATCY 7 cut(s) 84, 151, 331, 845, 1285, 2365, 3035
MhlI GDGCHC 5 cut(s) 315, 1062, 1420, 2387, 3620
MlsI TGGCCA 2 cut(s) 3876, 3953
MluI ACGCGT 1 cut(s) 2735
MluNI TGGCCA 2 cut(s) 3876, 3953
MlyI GAGTC 6 cut(s) 137, 194, 1963, 2125, 3254, 3695
MmeI TCCRAC 4 cut(s) 17, 553, 622, 3519
Mox20I TGGCCA 2 cut(s) 3876, 3953
Mph1103I ATGCAT 3 cut(s) 1458, 1598, 3139
MroXI GAANNNNTTC 4 cut(s) 580, 2277, 2915, 3679
MscI TGGCCA 2 cut(s) 3876, 3953
MslI CAYNNNNRTG 4 cut(s) 610, 1770, 2512, 3132
Msp20I TGGCCA 2 cut(s) 3876, 3953
MspA1I CMGCKG 1 cut(s) 3498
MspCI CTTAAG 1 cut(s) 2111
MspI CCGG 2 cut(s) 2994, 4061
MspR9I CCNGG 6 cut(s) 660, 1283, 1515, 2951, 2994, 3263
MunI CAATTG 4 cut(s) 2247, 2268, 2565, 2651
Mva1269I GAATGC 7 cut(s) 134, 287, 660, 946, 1598, 1714, 3578
MvaI CCWGG 5 cut(s) 660, 1283, 1515, 2951, 3263
MvnI CGCG 3 cut(s) 2682, 2737, 3972
MwoI GCNNNNNNNGC 8 cut(s) 131, 828, 1066, 1453, 1519, 1583, 2837, 3101
NciI CCSGG 1 cut(s) 2994
NcoI CCATGG 1 cut(s) 3275
NdeI CATATG 3 cut(s) 3098, 3255, 3268
NlaIV GGNNCC 7 cut(s) 312, 847, 1287, 2686, 3037, 3895, 3984
NmeAIII GCCGAG 1 cut(s) 394
NmuCI GTSAC 4 cut(s) 2516, 2612, 3500, 3795
NsbI TGCGCA 1 cut(s) 918
NsiI ATGCAT 3 cut(s) 1458, 1598, 3139
NspI RCATGY 4 cut(s) 781, 1323, 1769, 1885
PaeR7I CTCGAG 1 cut(s) 3583
PagI TCATGA 3 cut(s) 1795, 2812, 3490
PciI ACATGT 1 cut(s) 1765
PciSI GCTCTTC 1 cut(s) 3447
PctI GAATGC 7 cut(s) 134, 287, 660, 946, 1598, 1714, 3578
PdmI GAANNNNTTC 4 cut(s) 580, 2277, 2915, 3679
PfoI TCCNGGA 1 cut(s) 2992
PkrI GCNGC 8 cut(s) 7, 292, 2129, 2171, 2598, 2679, 2822, 4036
PleI GAGTC 6 cut(s) 137, 194, 1962, 2124, 3253, 3694
PpsI GAGTC 6 cut(s) 137, 194, 1962, 2124, 3253, 3694
Ppu21I YACGTR 1 cut(s) 589
PpuMI RGGWCCY 2 cut(s) 2215, 3982
PscI ACATGT 1 cut(s) 1765
PsiI TTATAA 2 cut(s) 399, 1689
Psp124BI GAGCTC 3 cut(s) 1062, 1420, 3620
Psp5II RGGWCCY 2 cut(s) 2215, 3982
Psp6I CCWGG 5 cut(s) 658, 1281, 1513, 2949, 3261
PspGI CCWGG 5 cut(s) 658, 1281, 1513, 2949, 3261
PspN4I GGNNCC 7 cut(s) 312, 847, 1287, 2686, 3037, 3895, 3984
PspPI GGNCC 3 cut(s) 2215, 3610, 3982
PspPPI RGGWCCY 2 cut(s) 2215, 3982
PstI CTGCAG 1 cut(s) 2822
PsuI RGATCY 7 cut(s) 84, 151, 331, 845, 1285, 2365, 3035
PvuII CAGCTG 1 cut(s) 3498
RsaI GTAC 7 cut(s) 2527, 2775, 2904, 3051, 3191, 3628, 4027
RsaNI GTAC 7 cut(s) 2526, 2774, 2903, 3050, 3190, 3627, 4026
RseI CAYNNNNRTG 4 cut(s) 610, 1770, 2512, 3132
SacI GAGCTC 3 cut(s) 1062, 1420, 3620
SapI GCTCTTC 1 cut(s) 3447
SatI GCNGC 8 cut(s) 6, 291, 2128, 2170, 2597, 2678, 2821, 4035
Sau96I GGNCC 3 cut(s) 2215, 3610, 3982
SchI GAGTC 6 cut(s) 137, 194, 1963, 2125, 3254, 3695
ScrFI CCNGG 6 cut(s) 660, 1283, 1515, 2951, 2994, 3263
SduI GDGCHC 5 cut(s) 315, 1062, 1420, 2387, 3620
SfcI CTRYAG 3 cut(s) 1578, 2311, 2818
Sfr274I CTCGAG 1 cut(s) 3583
SinI GGWCC 3 cut(s) 2215, 3610, 3982
SlaI CTCGAG 1 cut(s) 3583
SmiMI CAYNNNNRTG 4 cut(s) 610, 1770, 2512, 3132
SmlI CTYRAG 9 cut(s) 1055, 1117, 1140, 1801, 2111, 2173, 3242, 3583, 4029
SmoI CTYRAG 9 cut(s) 1055, 1117, 1140, 1801, 2111, 2173, 3242, 3583, 4029
SsiI CCGC 7 cut(s) 346, 651, 1492, 1586, 2682, 3150, 3608
SspI AATATT 3 cut(s) 804, 2576, 3937
SstI GAGCTC 3 cut(s) 1062, 1420, 3620
StyD4I CCNGG 6 cut(s) 658, 1281, 1513, 2949, 2992, 3261
StyI CCWWGG 3 cut(s) 3275, 3773, 4009
TaiI ACGT 4 cut(s) 591, 1629, 1782, 2968
TaqI TCGA 5 cut(s) 1613, 1727, 3072, 3584, 3931
TatI WGTACW 5 cut(s) 2525, 3049, 3189, 3626, 4025
TscAI CASTG 6 cut(s) 1009, 1996, 2377, 3337, 3478, 3561
TseFI GTSAC 4 cut(s) 2516, 2612, 3500, 3795
TseI GCWGC 8 cut(s) 5, 290, 2127, 2169, 2596, 2677, 2820, 4034
Tsp45I GTSAC 4 cut(s) 2516, 2612, 3500, 3795
TspGWI ACGGA 2 cut(s) 2535, 4030
TspRI CASTG 6 cut(s) 1009, 1996, 2377, 3337, 3478, 3561
Vha464I CTTAAG 1 cut(s) 2111
VpaK11BI GGWCC 3 cut(s) 2215, 3610, 3982
XagI CCTNNNNNAGG 1 cut(s) 663
XapI RAATTY 9 cut(s) 533, 1248, 1359, 1940, 2226, 2419, 3003, 3658, 3679
XbaI TCTAGA 1 cut(s) 797
XceI RCATGY 4 cut(s) 781, 1323, 1769, 1885
XcmI CCANNNNNNNNNTGG 2 cut(s) 856, 950
XhoI CTCGAG 1 cut(s) 3583
XmiI GTMKAC 1 cut(s) 1629
XmnI GAANNNNTTC 4 cut(s) 580, 2277, 2915, 3679
ZraI GACGTC 1 cut(s) 1627
Zsp2I ATGCAT 3 cut(s) 1458, 1598, 3139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.