Rmu_sc0034725.1_g000002

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0034725.1
OG0000067
Physical Location & Seq
Reverse (-)
7668 .. 12731
5064 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0034725.1_g000002.1.cds

Sequence Viewer

Length: 3453 bp
atgataaaggaaaggctaggtttgaaaaaagtcttggttgttcttgatgctgtggaaacttttgcccaaatagaagccttgcttggaaaactacatgcatttggtgagggaagtaggctcattgtaacaactagaaataagcaatcgctaagtggagttaatgagacgttcaagccatcgtatttaagggatgatgaagctctcgagctgtttcggaaatatgctttcagaacaaatcaacccaacagagactacgatgatctatcaaggcgtgccatagaatatgctcaaggtttgcctttagcacttaaagtcttgggggcatttcttgataacagaagtgtgtgtgaatgggaagtcgtgttggagaaattgaagaaaatcccacacatagaaattcaggatgtgcttagaacaagctttgatggactagacgattcagagaaaaacatattcctagatattgcatgtgtctttagaaaatggcgcacaaattttgtaacggaattactggacagttttggcttctttccccacagtggattaagagttctagttgacagagctctcataacttcgtcaaattacagagtcgacatgcatgatttatttcaagaaatgggtagagaaatcgtccgcctagaatctactaaaaaacctgggaagcgtagcaggttgtggagttataaagatgttaatcacgtgttgactcaaaatacggctacggatgcaattgaatgcttaatgctagacttgtcaaactcaaaagttgacctaagcataaatgctgaggcttttgtaaggatgacgaagctaagactgctaataatctgttattcattccacgtataccttattccatatagcgagtgccgcaagaatatatgcccagtaggtggctgtaaacaactcttgagcggggacttcgagtttctttcccatgaattgaggtttctcttatggcatggatgcccggtaaagtgtttgccatcaacttttatcccgaagaatcttgttcaccttgacatgcgtggtagtcacattcgagaactttgggaaggaatcaaacccctacaaaatctgagagtcatcaagttaagtcactgtcgataccttgtcaaaatccccgacttaactgaggcgataaatcttgagacactgcttcttgatggctgttcaagtttatttgaggttcattcatccatttctgctcttcaaaaccttcatttcttggatctaaaggggtgcaagcaacttaagattctgccaagctgcattcatatgaaatctctacggatccttcgtctatctggctgttcaaatctcgaaaagtttccagagattggagacgttatgacggagctaaaatggctttatttagatgggactggaattaaagaactgccctcatctattaacagtctcactgggcttgtagaattgaatctcaaaggttgcacagaacttaagactctagcaaccagcgttcatatgagttctcttcggaaacttaacctcgatggctgctcaaatcttgaaaagtttccggagatttcaggaatcatgaaggagctatcaatgcttcgtttagatgggaccgcaattaaaggactgccctcatcaatcgatctgcttctgaggattaaaaaattaagtgtgcggaactgcaaaagccttgaatttcttcccgacagtatctgtaatttggcaaaccttacacatctgaatctgtctgggtgctcggtacttcattacttgcctgagaaattgggggacttaaaatccttagaggtcctcgatgtaggatatagtggtataaaacaacttccattctctgtcctacgtttgggaaagcttaaaggtggattatcatgtattggatgtaaaaagatgacggcacccttgtcagcctggccaacatcaatcgaagaatattgtagttactctgttctgctacgtcttgatttaagtgactgcaatctgttgcagttatcggatggtattgctcatttatattcattgaaaacattaaagctgtgcagaaacaatttggagagcttacctgtgacaatgaatcgacttcgttgtctaacgcatcttgaattggaagcttgtaagcggctgaagtcagtacctgagctttcaccgagtgtaatacgcatagatgcacatgattgcacagctctggaaacagtttcaagacgaaagactgatccagtatgtagaattctagacttcaggttttctaattgcttccggcttcaagcaaatttgtttatagatattgttgagactgagacttattctctcgaccaggacagtcatccgcgacctctgtggttccacatgtggcttcctggaagtgaagttccagaatggttcaaccttcgggatacaggatgttcagaaacttttcggaagacaggatgttcagcaactttccaagcaccactgaatagtttcaataacaagcttttaggatttgctatatgcattgttattaaattcaatggtgctcgtcatgatgtatctgatatgtatggaagatgttattggacctcaaaaggaaagcatggtgagtacttcttcgaatttgatttgctcggccggcagttcggaatttacagtatagagtcagatcacctgttcatggcatatttgccatggtctggatatggcaggaattcacaagatagattagatgcatttaagattgtagtagaaactggaacttattctccgacagaagattggtttactactggctgcggtgttcgtttagtttatgctcacgatgagatgcaaaatagcgtgacacagcccataattcatgtggatggccagacaatggaggatgcggtgcagcagggggaggggtgctgtgctataggaggggtatcggggcttggtgcacctttctttacggaccagttggaaataagaaagagtgaaacagagttggaggcagagtcatcggagtcgcgtgaggggagaggcacgtgccaaatattggagctgttaccggagccgcagactgagagaaaagaagaggaggatggggaggggtgtcaggtcactcaggtgttagggtcggcattctctttgagaaagagagaaacagaaagagaaggagacagagtcacaagagtctctgacgagggagagatatcggagttgggcagccacagtggtctggtggtgccaaattcacagagtgagggcaaagaagagaaagaattgatgggggaggagtcgttggagagggttgttagggccgctgtgcagctgcttttactggctgggttctatggcagtagttctgcagtgtcttctgagacggtggatgcctctgggcctcacgcctttattgggatttgggattgtgctggggttgttgctatgatagggatattgctcgactttcggatagcagcgaggatttgtcaaggatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1150

Amino Acids

129.32

Weight (kDa)

6.09

Isoelectric Point (pI)

40.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 687
AasI GACNNNNNNGTC 1 cut(s) 2085
Acc36I ACCTGC 1 cut(s) 663
AccB1I GGYRCC 2 cut(s) 1897, 3199
AccB7I CCANNNNNTGG 5 cut(s) 896, 1343, 2669, 2848, 3010
AccBSI CCGCTC 1 cut(s) 918
AccI GTMKAC 2 cut(s) 594, 849
AccII CGCG 2 cut(s) 2329, 2983
AccIII TCCGGA 1 cut(s) 1546
AclWI GGATC 4 cut(s) 1242, 1291, 1304, 2207
AcoI YGGCCR 3 cut(s) 1913, 2605, 2839
AcuI CTGAAG 2 cut(s) 2144, 2221
AcvI CACGTG 2 cut(s) 703, 3000
AdeI CACNNNGTG 2 cut(s) 2150, 3215
AfaI GTAC 3 cut(s) 1746, 2133, 2582
AfiI CCNNNNNNNGG 9 cut(s) 539, 896, 1343, 1846, 2650, 2669, 2848, 3010, 3369
AflII CTTAAG 2 cut(s) 1256, 1466
AflIII ACRYGT 2 cut(s) 702, 2346
AjnI CCWGG 4 cut(s) 658, 1910, 2313, 2356
AjuI GAANNNNNNNTTGG 6 cut(s) 17, 49, 66, 98, 2536, 2568
AleI CACNNNNGTG 1 cut(s) 3080
AloI GAACNNNNNNTCC 6 cut(s) 534, 566, 2352, 2384, 2722, 2754
Alw21I GWGCWC 4 cut(s) 568, 1742, 2518, 2914
Alw44I GTGCAC 1 cut(s) 2910
AlwI GGATC 4 cut(s) 1242, 1291, 1304, 2207
AlwNI CAGNNNCTG 2 cut(s) 1698, 2135
Ama87I CYCGRG 1 cut(s) 203
Aor13HI TCCGGA 1 cut(s) 1546
AoxI GGCC 5 cut(s) 1913, 2605, 2839, 3273, 3353
ApaLI GTGCAC 1 cut(s) 2910
ApeKI GCWGC 8 cut(s) 1272, 1524, 2766, 2863, 3180, 3283, 3286, 3431
Asp700I GAANNNNTTC 6 cut(s) 1332, 1542, 1683, 2412, 2459, 2734
AspLEI GCGC 1 cut(s) 489
AspS9I GGNCC 6 cut(s) 1596, 1792, 2556, 2926, 3273, 3353
AsuC2I CCSGG 1 cut(s) 974
AsuHPI GGTGA 5 cut(s) 116, 1010, 2136, 2588, 2633
AsuII TTCGAA 1 cut(s) 2589
AvaI CYCGRG 1 cut(s) 203
AvaII GGWCC 4 cut(s) 1596, 1792, 2556, 2926
BaeGI GKGCMC 1 cut(s) 2914
BalI TGGCCA 2 cut(s) 1915, 2841
BamHI GGATCC 1 cut(s) 1296
BanI GGYRCC 2 cut(s) 1897, 3199
BanII GRGCYC 1 cut(s) 568
BbrPI CACGTG 2 cut(s) 703, 3000
BbsI GAAGAC 2 cut(s) 2426, 3321
Bbv12I GWGCWC 4 cut(s) 568, 1742, 2518, 2914
BbvCI CCTCAGC 1 cut(s) 789
BbvI GCAGC 8 cut(s) 1259, 1511, 2753, 2875, 3192, 3273, 3295, 3443
BceAI ACGGC 2 cut(s) 735, 1911
BcgI CGANNNNNNTGC 2 cut(s) 2955, 2989
BciT130I CCWGG 4 cut(s) 660, 1912, 2315, 2358
BciVI GTATCC 1 cut(s) 2386
BcnI CCSGG 1 cut(s) 974
BfaI CTAG 9 cut(s) 17, 132, 431, 458, 554, 641, 749, 1475, 2231
BfmI CTRYAG 2 cut(s) 2886, 3321
BfrI CTTAAG 2 cut(s) 1256, 1466
BfuAI ACCTGC 1 cut(s) 663
BfuI GTATCC 1 cut(s) 2386
BmcAI AGTACT 1 cut(s) 2582
Bme1390I CCNGG 5 cut(s) 660, 974, 1912, 2315, 2358
Bme18I GGWCC 4 cut(s) 1596, 1792, 2556, 2926
BmeT110I CYCGRG 1 cut(s) 203
BmgT120I GGNCC 6 cut(s) 1596, 1792, 2556, 2926, 3273, 3353
BmiI GGNNCC 6 cut(s) 1298, 1597, 1899, 2342, 3027, 3201
BmrFI CCNGG 5 cut(s) 660, 974, 1912, 2315, 2358
BmrI ACTGGG 2 cut(s) 884, 1437
BmsI GCATC 9 cut(s) 37, 718, 959, 2104, 2155, 2692, 2790, 2845, 3334
BmuI ACTGGG 2 cut(s) 884, 1437
BpiI GAAGAC 2 cut(s) 2426, 3321
Bpu10I CCTNAGC 3 cut(s) 776, 789, 2136
Bpu14I TTCGAA 1 cut(s) 2589
BpuEI CTTGAG 3 cut(s) 273, 934, 1172
BpuMI CCSGG 1 cut(s) 974
Bsa29I ATCGAT 1 cut(s) 1626
BsaAI YACGTR 3 cut(s) 703, 847, 3000
BsaBI GATNNNNATC 1 cut(s) 696
BsaJI CCNNGG 2 cut(s) 659, 2663
BsaWI WCCGGW 2 cut(s) 1546, 3022
BsaXI ACNNNNNCTCC 8 cut(s) 2722, 2752, 2954, 2984, 3123, 3153, 3239, 3269
Bsc4I CCNNNNNNNGG 9 cut(s) 539, 896, 1343, 1846, 2650, 2669, 2848, 3010, 3369
Bse118I RCCGGY 1 cut(s) 2607
Bse1I ACTGG 9 cut(s) 516, 890, 1393, 1432, 2216, 2731, 2767, 2929, 3300
Bse8I GATNNNNATC 1 cut(s) 696
BseAI TCCGGA 1 cut(s) 1546
BseBI CCWGG 4 cut(s) 660, 1912, 2315, 2358
BseCI ATCGAT 1 cut(s) 1626
BseDI CCNNGG 2 cut(s) 659, 2663
BseJI GATNNNNATC 1 cut(s) 696
BseLI CCNNNNNNNGG 9 cut(s) 539, 896, 1343, 1846, 2650, 2669, 2848, 3010, 3369
BseNI ACTGG 9 cut(s) 516, 890, 1393, 1432, 2216, 2731, 2767, 2929, 3300
BseRI GAGGAG 2 cut(s) 3065, 3263
BseSI GKGCMC 1 cut(s) 2914
BseX3I CGGCCG 1 cut(s) 2605
BseXI GCAGC 8 cut(s) 1259, 1511, 2753, 2875, 3192, 3273, 3295, 3443
BseYI CCCAGC 2 cut(s) 3299, 3386
BsgI GTGCAG 3 cut(s) 2059, 2882, 3302
Bsh1236I CGCG 2 cut(s) 2329, 2983
Bsh1285I CGRYCG 1 cut(s) 2608
BshFI GGCC 5 cut(s) 1915, 2607, 2841, 3275, 3355
BshNI GGYRCC 2 cut(s) 1897, 3199
BshVI ATCGAT 1 cut(s) 1626
BsiEI CGRYCG 1 cut(s) 2608
BsiHKAI GWGCWC 4 cut(s) 568, 1742, 2518, 2914
BsiHKCI CYCGRG 1 cut(s) 203
BsiSI CCGG 5 cut(s) 974, 1547, 2257, 2608, 3023
BslFI GGGAC 4 cut(s) 935, 1399, 1609, 1787
BslI CCNNNNNNNGG 9 cut(s) 539, 896, 1343, 1846, 2650, 2669, 2848, 3010, 3369
BsmBI CGTCTC 3 cut(s) 158, 1341, 3329
BsmFI GGGAC 4 cut(s) 935, 1399, 1609, 1787
BsmI GAATGC 3 cut(s) 743, 1275, 3095
BsnI GGCC 5 cut(s) 1915, 2607, 2841, 3275, 3355
BsoBI CYCGRG 1 cut(s) 203
Bsp119I TTCGAA 1 cut(s) 2589
Bsp1286I GDGCHC 4 cut(s) 568, 1742, 2518, 2914
Bsp13I TCCGGA 1 cut(s) 1546
Bsp143I GATC 6 cut(s) 259, 1234, 1296, 1627, 2212, 2638
Bsp19I CCATGG 1 cut(s) 2663
BspANI GGCC 5 cut(s) 1915, 2607, 2841, 3275, 3355
BspDI ATCGAT 1 cut(s) 1626
BspEI TCCGGA 1 cut(s) 1546
BspFNI CGCG 2 cut(s) 2329, 2983
BspHI TCATGA 2 cut(s) 1563, 2521
BspLI GGNNCC 6 cut(s) 1298, 1597, 1899, 2342, 3027, 3201
BspMAI CTGCAG 1 cut(s) 3325
BspMI ACCTGC 1 cut(s) 663
BspPI GGATC 4 cut(s) 1242, 1291, 1304, 2207
BspQI GCTCTTC 1 cut(s) 1218
BspT104I TTCGAA 1 cut(s) 2589
BspT107I GGYRCC 2 cut(s) 1897, 3199
BspTI CTTAAG 2 cut(s) 1256, 1466
BsrBI CCGCTC 1 cut(s) 918
BsrFI RCCGGY 1 cut(s) 2607
BsrI ACTGG 9 cut(s) 516, 890, 1393, 1432, 2216, 2731, 2767, 2929, 3300
BssAI RCCGGY 1 cut(s) 2607
BssECI CCNNGG 2 cut(s) 659, 2663
BssMI GATC 6 cut(s) 259, 1234, 1296, 1627, 2212, 2638
BssNAI GTATAC 1 cut(s) 850
BssT1I CCWWGG 1 cut(s) 2663
Bst1107I GTATAC 1 cut(s) 850
Bst2UI CCWGG 4 cut(s) 660, 1912, 2315, 2358
Bst6I CTCTTC 4 cut(s) 1218, 1506, 3042, 3222
BstAFI CTTAAG 2 cut(s) 1256, 1466
BstBAI YACGTR 3 cut(s) 703, 847, 3000
BstBI TTCGAA 1 cut(s) 2589
BstC8I GCNNGC 3 cut(s) 273, 1250, 2609
BstDSI CCRYGG 1 cut(s) 2663
BstFNI CGCG 2 cut(s) 2329, 2983
BstHHI GCGC 1 cut(s) 489
BstKTI GATC 6 cut(s) 262, 1237, 1299, 1630, 2215, 2641
BstMBI GATC 6 cut(s) 259, 1234, 1296, 1627, 2212, 2638
BstMCI CGRYCG 1 cut(s) 2608
BstMWI GCNNNNNNNGC 6 cut(s) 728, 820, 873, 1369, 1579, 2608
BstNI CCWGG 4 cut(s) 660, 1912, 2315, 2358
BstNSI RCATGY 5 cut(s) 98, 471, 601, 1030, 2350
BstSCI CCNGG 5 cut(s) 658, 972, 1910, 2313, 2356
BstSFI CTRYAG 2 cut(s) 2886, 3321
BstSLI GKGCMC 1 cut(s) 2914
BstUI CGCG 2 cut(s) 2329, 2983
BstV1I GCAGC 8 cut(s) 1259, 1511, 2753, 2875, 3192, 3273, 3295, 3443
BstV2I GAAGAC 2 cut(s) 2426, 3321
BstX2I RGATCY 2 cut(s) 1234, 1296
BstYI RGATCY 2 cut(s) 1234, 1296
BstZ17I GTATAC 1 cut(s) 850
BstZI CGGCCG 1 cut(s) 2605
Bsu15I ATCGAT 1 cut(s) 1626
BsuI GTATCC 1 cut(s) 2386
BsuRI GGCC 5 cut(s) 1915, 2607, 2841, 3275, 3355
BsuTUI ATCGAT 1 cut(s) 1626
BtgI CCRYGG 1 cut(s) 2663
BtsI GCAGTG 2 cut(s) 1157, 3330
BtsIMutI CAGTG 7 cut(s) 544, 1102, 1157, 1425, 2450, 3193, 3330
BveI ACCTGC 1 cut(s) 663
Cac8I GCNNGC 3 cut(s) 273, 1250, 2609
CaiI CAGNNNCTG 2 cut(s) 1698, 2135
CciI TCATGA 2 cut(s) 1563, 2521
CfoI GCGC 1 cut(s) 489
Cfr10I RCCGGY 1 cut(s) 2607
Cfr13I GGNCC 6 cut(s) 1596, 1792, 2556, 2926, 3273, 3353
ClaI ATCGAT 1 cut(s) 1626
Csp6I GTAC 3 cut(s) 1745, 2132, 2581
CviQI GTAC 3 cut(s) 1745, 2132, 2581
DpnI GATC 6 cut(s) 261, 1236, 1298, 1629, 2214, 2640
DpnII GATC 6 cut(s) 259, 1234, 1296, 1627, 2212, 2638
DraIII CACNNNGTG 2 cut(s) 2150, 3215
DrdI GACNNNNNNGTC 1 cut(s) 2085
DseDI GACNNNNNNGTC 1 cut(s) 2085
EaeI YGGCCR 3 cut(s) 1913, 2605, 2839
EagI CGGCCG 1 cut(s) 2605
Eam1104I CTCTTC 4 cut(s) 1218, 1506, 3042, 3222
EarI CTCTTC 4 cut(s) 1218, 1506, 3042, 3222
EciI GGCGGA 1 cut(s) 626
Ecl136II GAGCTC 1 cut(s) 566
EclXI CGGCCG 1 cut(s) 2605
Eco130I CCWWGG 1 cut(s) 2663
Eco24I GRGCYC 1 cut(s) 568
Eco32I GATATC 1 cut(s) 3168
Eco47I GGWCC 4 cut(s) 1596, 1792, 2556, 2926
Eco52I CGGCCG 1 cut(s) 2605
Eco53kI GAGCTC 1 cut(s) 566
Eco57I CTGAAG 2 cut(s) 2144, 2221
Eco72I CACGTG 2 cut(s) 703, 3000
Eco88I CYCGRG 1 cut(s) 203
EcoICRI GAGCTC 1 cut(s) 566
EcoO109I RGGNCCY 1 cut(s) 1792
EcoRI GAATTC 2 cut(s) 2226, 2683
EcoRII CCWGG 4 cut(s) 658, 1910, 2313, 2356
EcoRV GATATC 1 cut(s) 3168
EcoT14I CCWWGG 1 cut(s) 2663
EcoT22I ATGCAT 4 cut(s) 100, 603, 2495, 2707
EcoT38I GRGCYC 1 cut(s) 568
ErhI CCWWGG 1 cut(s) 2663
Esp3I CGTCTC 3 cut(s) 158, 1341, 3329
FalI AAGNNNNNCTT 2 cut(s) 66, 98
FaqI GGGAC 4 cut(s) 935, 1399, 1609, 1787
FauI CCCGC 1 cut(s) 911
FauNDI CATATG 2 cut(s) 1281, 1491
FblI GTMKAC 2 cut(s) 594, 849
FriOI GRGCYC 1 cut(s) 568
FspBI CTAG 9 cut(s) 17, 132, 431, 458, 554, 641, 749, 1475, 2231
GlaI GCGC 1 cut(s) 488
GsaI CCCAGC 2 cut(s) 3303, 3390
HaeIII GGCC 5 cut(s) 1915, 2607, 2841, 3275, 3355
HapII CCGG 5 cut(s) 974, 1547, 2257, 2608, 3023
HhaI GCGC 1 cut(s) 489
Hin6I GCGC 1 cut(s) 487
HinP1I GCGC 1 cut(s) 487
HincII GTYRAC 4 cut(s) 559, 595, 708, 772
HindII GTYRAC 4 cut(s) 559, 595, 708, 772
HindIII AAGCTT 4 cut(s) 418, 1853, 2109, 2471
HpaII CCGG 5 cut(s) 974, 1547, 2257, 2608, 3023
HphI GGTGA 5 cut(s) 116, 1010, 2136, 2588, 2633
Hpy166II GTNNAC 9 cut(s) 559, 595, 708, 772, 850, 905, 1018, 2757, 2912
Hpy8I GTNNAC 9 cut(s) 559, 595, 708, 772, 850, 905, 1018, 2757, 2912
HpyAV CCTTC 6 cut(s) 1052, 1232, 1310, 1561, 2396, 3122
HpyCH4IV ACGT 7 cut(s) 167, 702, 846, 1350, 1843, 1957, 2999
HpyF10VI GCNNNNNNNGC 6 cut(s) 728, 820, 873, 1369, 1579, 2608
HpySE526I ACGT 7 cut(s) 167, 702, 846, 1350, 1843, 1957, 2999
HspAI GCGC 1 cut(s) 487
Kpn2I TCCGGA 1 cut(s) 1546
KroI GCCGGC 1 cut(s) 2607
KroNI GCCGGC 1 cut(s) 2609
Kzo9I GATC 6 cut(s) 259, 1234, 1296, 1627, 2212, 2638
LguI GCTCTTC 1 cut(s) 1218
LmnI GCTCC 4 cut(s) 1360, 1570, 3013, 3025
Lsp1109I GCAGC 8 cut(s) 1259, 1511, 2753, 2875, 3192, 3273, 3295, 3443
LweI GCATC 9 cut(s) 37, 718, 959, 2104, 2155, 2692, 2790, 2845, 3334
MaeI CTAG 9 cut(s) 17, 132, 431, 458, 554, 641, 749, 1475, 2231
MaeII ACGT 7 cut(s) 167, 702, 846, 1350, 1843, 1957, 2999
MalI GATC 6 cut(s) 261, 1236, 1298, 1629, 2214, 2640
MbiI CCGCTC 1 cut(s) 918
MboI GATC 6 cut(s) 259, 1234, 1296, 1627, 2212, 2638
MfeI CAATTG 1 cut(s) 732
MflI RGATCY 2 cut(s) 1234, 1296
MhlI GDGCHC 4 cut(s) 568, 1742, 2518, 2914
MlsI TGGCCA 2 cut(s) 1915, 2841
MluNI TGGCCA 2 cut(s) 1915, 2841
MmeI TCCRAC 5 cut(s) 345, 2765, 2913, 2940, 3237
Mox20I TGGCCA 2 cut(s) 1915, 2841
Mph1103I ATGCAT 4 cut(s) 100, 603, 2495, 2707
MroI TCCGGA 1 cut(s) 1546
MroNI GCCGGC 1 cut(s) 2607
MroXI GAANNNNTTC 6 cut(s) 1332, 1542, 1683, 2412, 2459, 2734
MscI TGGCCA 2 cut(s) 1915, 2841
MslI CAYNNNNRTG 3 cut(s) 1280, 2835, 3080
Msp20I TGGCCA 2 cut(s) 1915, 2841
MspA1I CMGCKG 2 cut(s) 3278, 3286
MspCI CTTAAG 2 cut(s) 1256, 1466
MspI CCGG 5 cut(s) 974, 1547, 2257, 2608, 3023
MspR9I CCNGG 5 cut(s) 660, 974, 1912, 2315, 2358
MunI CAATTG 1 cut(s) 732
Mva1269I GAATGC 3 cut(s) 743, 1275, 3095
MvaI CCWGG 4 cut(s) 660, 1912, 2315, 2358
MvnI CGCG 2 cut(s) 2329, 2983
MwoI GCNNNNNNNGC 6 cut(s) 728, 820, 873, 1369, 1579, 2608
NaeI GCCGGC 1 cut(s) 2609
NciI CCSGG 1 cut(s) 974
NcoI CCATGG 1 cut(s) 2663
NdeI CATATG 2 cut(s) 1281, 1491
NdeII GATC 6 cut(s) 259, 1234, 1296, 1627, 2212, 2638
NgoMIV GCCGGC 1 cut(s) 2607
NlaIV GGNNCC 6 cut(s) 1298, 1597, 1899, 2342, 3027, 3201
NmeAIII GCCGAG 1 cut(s) 2583
NmuCI GTSAC 7 cut(s) 1037, 1100, 1970, 2065, 2812, 3073, 3139
NsiI ATGCAT 4 cut(s) 100, 603, 2495, 2707
NspI RCATGY 5 cut(s) 98, 471, 601, 1030, 2350
NspV TTCGAA 1 cut(s) 2589
OliI CACNNNNGTG 1 cut(s) 3080
PaeR7I CTCGAG 1 cut(s) 203
PagI TCATGA 2 cut(s) 1563, 2521
PciI ACATGT 1 cut(s) 2346
PciSI GCTCTTC 1 cut(s) 1218
PcsI WCGNNNNNNNCGW 1 cut(s) 1300
PctI GAATGC 3 cut(s) 743, 1275, 3095
PdiI GCCGGC 1 cut(s) 2609
PdmI GAANNNNTTC 6 cut(s) 1332, 1542, 1683, 2412, 2459, 2734
PfeI GAWTC 9 cut(s) 437, 644, 1009, 1062, 1261, 1444, 1560, 1726, 2074
PflFI GACNNNGTC 1 cut(s) 3137
PflMI CCANNNNNTGG 5 cut(s) 896, 1343, 2669, 2848, 3010
PfoI TCCNGGA 1 cut(s) 2356
PmaCI CACGTG 2 cut(s) 703, 3000
PmlI CACGTG 2 cut(s) 703, 3000
Ppu21I YACGTR 3 cut(s) 703, 847, 3000
PpuMI RGGWCCY 1 cut(s) 1792
PscI ACATGT 1 cut(s) 2346
PsiI TTATAA 1 cut(s) 687
Psp124BI GAGCTC 1 cut(s) 568
Psp5II RGGWCCY 1 cut(s) 1792
Psp6I CCWGG 4 cut(s) 658, 1910, 2313, 2356
PspCI CACGTG 2 cut(s) 703, 3000
PspFI CCCAGC 2 cut(s) 3299, 3386
PspGI CCWGG 4 cut(s) 658, 1910, 2313, 2356
PspN4I GGNNCC 6 cut(s) 1298, 1597, 1899, 2342, 3027, 3201
PspPI GGNCC 6 cut(s) 1596, 1792, 2556, 2926, 3273, 3353
PspPPI RGGWCCY 1 cut(s) 1792
PstI CTGCAG 1 cut(s) 3325
PstNI CAGNNNCTG 2 cut(s) 1698, 2135
PsuI RGATCY 2 cut(s) 1234, 1296
PsyI GACNNNGTC 1 cut(s) 3137
PvuII CAGCTG 1 cut(s) 3286
RsaI GTAC 3 cut(s) 1746, 2133, 2582
RsaNI GTAC 3 cut(s) 1745, 2132, 2581
RseI CAYNNNNRTG 3 cut(s) 1280, 2835, 3080
SacI GAGCTC 1 cut(s) 568
SalI GTCGAC 1 cut(s) 593
SapI GCTCTTC 1 cut(s) 1218
Sau3AI GATC 6 cut(s) 259, 1234, 1296, 1627, 2212, 2638
Sau96I GGNCC 6 cut(s) 1596, 1792, 2556, 2926, 3273, 3353
ScaI AGTACT 1 cut(s) 2582
ScrFI CCNGG 5 cut(s) 660, 974, 1912, 2315, 2358
SduI GDGCHC 4 cut(s) 568, 1742, 2518, 2914
SfaNI GCATC 9 cut(s) 37, 718, 959, 2104, 2155, 2692, 2790, 2845, 3334
SfcI CTRYAG 2 cut(s) 2886, 3321
Sfr274I CTCGAG 1 cut(s) 203
SfuI TTCGAA 1 cut(s) 2589
SinI GGWCC 4 cut(s) 1596, 1792, 2556, 2926
SlaI CTCGAG 1 cut(s) 203
SmiMI CAYNNNNRTG 3 cut(s) 1280, 2835, 3080
SmlI CTYRAG 6 cut(s) 203, 288, 913, 1151, 1256, 1466
SmoI CTYRAG 6 cut(s) 203, 288, 913, 1151, 1256, 1466
SspI AATATT 2 cut(s) 1934, 3009
SspMI CTAG 9 cut(s) 17, 132, 431, 458, 554, 641, 749, 1475, 2231
SstI GAGCTC 1 cut(s) 568
StyD4I CCNGG 5 cut(s) 658, 972, 1910, 2313, 2356
StyI CCWWGG 1 cut(s) 2663
TaiI ACGT 7 cut(s) 170, 705, 849, 1353, 1846, 1960, 3002
TatI WGTACW 1 cut(s) 2580
TauI GCSGC 4 cut(s) 876, 2122, 3031, 3278
TfiI GAWTC 9 cut(s) 437, 644, 1009, 1062, 1261, 1444, 1560, 1726, 2074
TscAI CASTG 7 cut(s) 544, 1109, 1164, 1432, 2457, 3193, 3330
TseFI GTSAC 7 cut(s) 1037, 1100, 1970, 2065, 2812, 3073, 3139
TseI GCWGC 8 cut(s) 1272, 1524, 2766, 2863, 3180, 3283, 3286, 3431
Tsp45I GTSAC 7 cut(s) 1037, 1100, 1970, 2065, 2812, 3073, 3139
TspGWI ACGGA 5 cut(s) 518, 740, 1309, 1373, 2939
TspRI CASTG 7 cut(s) 544, 1109, 1164, 1432, 2457, 3193, 3330
Tth111I GACNNNGTC 1 cut(s) 3137
Van91I CCANNNNNTGG 5 cut(s) 896, 1343, 2669, 2848, 3010
Vha464I CTTAAG 2 cut(s) 1256, 1466
VneI GTGCAC 1 cut(s) 2910
VpaK11BI GGWCC 4 cut(s) 1596, 1792, 2556, 2926
XbaI TCTAGA 1 cut(s) 2230
XceI RCATGY 5 cut(s) 98, 471, 601, 1030, 2350
XcmI CCANNNNNNNNNTGG 1 cut(s) 2830
XhoI CTCGAG 1 cut(s) 203
XmiI GTMKAC 2 cut(s) 594, 849
XmnI GAANNNNTTC 6 cut(s) 1332, 1542, 1683, 2412, 2459, 2734
XspI CTAG 9 cut(s) 17, 132, 431, 458, 554, 641, 749, 1475, 2231
ZrmI AGTACT 1 cut(s) 2582
Zsp2I ATGCAT 4 cut(s) 100, 603, 2495, 2707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.