Rw1G035850

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000067
Physical Location & Seq
Forward (+)
63636915 .. 63641748
4834 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G035850.1

Sequence Viewer

Length: 3885 bp
ATGGCCGCTTCTTCATCTTCATCTTCTTCTGGTGGTCATTGGAAGTATGATGTCTTCCTCAGTTTTCGAGGGGAGGACACTCGCAAAAACATCGTTTGCCATCTTTACAAAGCTCTAGAACAGAAAGCAATCCACACCTTCATCGATTCTGAAGAGCTCGAAAAAGGCAACAAAATTTCCGAGCTACTCAACGCTGTTGGCGAATCGAGGATTTCGGTTGTAGTTTTATCTCAAAACTATGCCACTTCCAGTTGGTGCTTAAAAGAGCTCGTCAAAATCATGGAATGCGAGGATAAGAAGCAGCAGATTGTGGTGCCCATTTTCTATGAAGTAGATCCCTCCGATATTCGAAAATTGGAGAAAATGTTTGGGGAAGCTTTTGCCAAACATGAACAAAATCCTAACCTCGACAAGGAAGTATTGGAGACCTGGAAAACTGCACTAAAAAGAATCACGGATCTGTCTGGCTGGGATTCGCGACAGTATAAGGATGACTTTGAGCTTATTGAGAAAATTGTAGGAGACATATTAGAAAAACGGATTCACATCCCCCGACCCGCCAGTCTAGCCAGAAATTTGGTCAGAATGGATCATCACATTAAGGAAATGAGTTTGCGGTTATGTCGTGGGGTGAATGATGTTGGTGTTGTTGGAATATGGGGTATGGGCGGTATAGGCAAAACAACCATAGCTAGAGCTGTTTATGACAATATAGTTCATCTATTTGAACATAAGTGCTTTTTTTATAATGTGAAAGAAGGTTTTGCGAAGAAGAGTGAAGTACAAATGCGGGGAGAACTTCTGTCTACAATCTTGGGGGGAAATGTGCAGAGCAAAAAAGTTCTAGTTGTTCTTGATGACGTGGAAACATCTTCCCAAATTGAAGCCTTACTTGGAGATTTTTATTCATTTGGTGGTGGAAGTAGAATCCTTGTAACAACTAGAGATAAGGAATCACTAGCTGTCACTGGAGTTGATGAGACATATGAGCCCAGGTGTTTAAGTCATAATGAATCTCTTGAACTCTTTATGAAACATGCCTTCAGAACAAACCAACCCAGTAAAGAGTATAGTGATCTCTCAAACCAAGCCATAGAGTATGCTCAGGGTCTGCCTTTAGCACTTAAAGTCTTGGGAGCTTCTCTTTATGACAAAAGTATACACCAGTGGAAAGAGTTGTTAGAGAAAATCAAGAAAATCCCGGAAAGGAAGATTATGGATGTGCTTCGAACAAGCTTTGAGGGACTAGACGAATCACAGAAGGACATATTTCTAGACATCGCATGTCTCTTTAGAGAATGGGGCGAAGATTTTGTAACAGAAGTACTAGACAGTTGTGGCTTCTATCCCCACAATGGATTAAGAGTTCTAGTTGACAGAGCACTCATAATTACTCATAGATTTATGGGACTCGACATGCATGATTTACTACAAGATATGGGTAAAGAAATTGTCCACCTAGAATCTACTAAAGAAGCTGGGAAGCGCAGTAGGTTGTGGAGTTGTGATGATGTTAATCATGTATTGACTCAAAATACGGCAACGGATGCAGTTGAATGCTTAATGCTAGACTTGTCGAACTCAAAAGTCGACCTATGCATACATACTGGAGCTTTTGTTAGAATGACGAAGCTAAGACCACTCATAATCTATTATTCATTCTTCAATACACATGATTACTTTGGCTTTTTCAATGATGACAATAAGGATGAATACTATCTTGGATATGTTAGAAGCTGTGAGGATTTATGTCCTGTTGATGGCTGTAAACAACTCTGGAGGGGGGACTTGGAGTATCTTTCTCAAGAATTGAGGTTTCTCTTATGGCATGGATGCCCGCTAAAGTCTTTGCCGTCAACCTTTATCCCGAAGAATCTTGTTCAACTTGACATGCGTGGTAGTCACGTTCAACAACTTTGGGAAGGAATCAAGCCTCTACAAAATTTGAGAGTCATCAAGTTAAGTTACTGTCAATACCTTGTCACAATCCCTGACTTAACTGAGGCAATAAATCTTGAGAAAATTTATCTTGCTGGCTGTTCAAGTTTATTTGAGGTTCATCCATCCATTTCTGCTCTTCAAAACCTTCGTGTCTTGAATCTAATGGGGTGCAAGCAACTTAAGATTCTGCCAAGCTGCATTCTTCTGAAATCTCTAGGGATCCTTCGTCTTTCTGGCTGTTCAAATCTTGAAAAGTTTCCAGAGATTTCAGAGGTTATGACAAAGCTAAAATGGCTTTATTTAGATGGAACTGCAATTAAAGGAGTACCCTCATCTCTTAATAATCTCACTGGGCTTCTTGTTTTAGATCTCAAGGATTGCACAGAACTTAAGACCCTACCAACCAGCATTCATATGAGTTCTCTTAGGACCCTTAACCTCGATGGCTGCTCAAGTCTTGAAAAGTTTCCGGAGATTTTAGGAATCATGAAGGAGCTACAAGAGCTTCATTTAGATGGGACCACAATTAAAGAACTACCCTCCTCAATTGATCTACTTCTGGGGATTGGAAAATTAAGTATGCGGAACTGCAAAAGCCTTGAATTTCTTCTTGACAATATCTGTAATTTGGTAAACCTTACACATCTCGATCTGTCTGGGTGCTCAGTACTTCATAACTTGCCTGAGAAATTGGGGGACTTAGAATCCTTCCAGGTCCTTGACCTGTGCAGAAACAATTTGGAGAGCTTACCTGTGATAATGAATCAACTTAGTTGGTTAACACATCTTGAATTGGAAGCTTGCAAGAGGCTAAAATCAATACCAGAGCTTCCATCGAGTATAAATTACATAGATGCGCATGATTGCACAGCTTTGGAAACAGTTTCAAAGCCACAGCCTCAGTACCACCTGTCGAATCATTTCTTTACATTTTCTAATTGCCTCCAACTGGTACAAACAAATCTATTTACAGATATTGTGGAAAGACATTCACATTTGCAGGATGACTACCTGCGACCTCTTCGGTTGAAAATATCTCTTCCTGGAAGTGAAGTTCCCAAATGGTTCGACCATCAGACGAGTGGATTTTCAGTGAGTTTTTTGGGATTTGCTATCTGTGCTGTTCGTAATTGCCAGGGAATTAATGATGATACATCTCCTTTATCTGCTTCTTGTTTATGCACCTTCAAAGGAAATCGTGGCAAGTGTAGTTTCAGTTTTGATTTACTCAATTCAGTTTTTAGAACTGATAGATTATTTAGCTCAGATCACATGTTCCTGCGATATGTGTCGTGGTCTGATTGTCGCCTGATTGAAGAAGGAAAGCTGGTCAATGAAGCCTACACTGAGGCCACATTTCAGATTGTAGTAAGTGAATCTATAACAGAACAACTCCGCATCAGAAGCTGCGGAGTTCGTTTTGTATATGCTAATCCAGATGAAATGCTGGATCTAAACATGCCGCAGCCCCAAGTTCATGTGGACAGTTGTGAGACTGGAGTTTGTTTTATGGACCCATATGAATTGAGAAACAATGAAACAGAAAGGGTAGAAATTTTGAGGGAGATAGAGACGTCAAGTAACGGACTTCATCTCTTTCATGGTAATTATATGAAAGTGCAAAATGAAAGCTTGACTCGGCCCACAATTCATGTTGAAGGACTTGGAGCCCCTATCTTGCCGGACCAGTTGAAAATAAGAATTAATGAAACAGAGTGCGAGGCAGAGTCGTCGGAGTCGGGTGAAGGGAGAGGCATGTACCAAACATTGGAGCTGTTACCAGAGCCACAGACAGAAAGAGCAGAAGAGGAGGAGATAATGGTGGTGGCAGTGCATGGGAACGGTTGCCGGGTGTTAGGGCCAGTATGCTGCTGCTTTTCATTGTCTGGATGCGCTATGTCTTTGAGAAACAGAAACAGAAAGAGAAGGAGACAGAGTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1294

Amino Acids

146.87

Weight (kDa)

5.69

Isoelectric Point (pI)

47.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 15 - 186 9.1e-52 TIR domain
TIR_2 PF13676 18 - 114 3.6e-15 TIR domain
NB-ARC PF00931 198 - 351 8.3e-16 NB-ARC domain
WHD_ROQ1 PF23282 418 - 486 2.2e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 708 - 819 5.7e-09 Leucine-rich repeat region
LRR_13 PF23286 716 - 786 6.5e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 786 - 872 1.6e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 839 - 921 7.3e-10 Leucine-rich repeat region
C-JID PF20160 994 - 1126 5.8e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 747
AasI GACNNNNNNGTC 1 cut(s) 561
AatII GACGTC 1 cut(s) 3518
Acc16I TGCGCA 1 cut(s) 2799
Acc36I ACCTGC 1 cut(s) 2961
AccB1I GGYRCC 1 cut(s) 313
AccB7I CCANNNNNTGG 1 cut(s) 3709
AccI GTMKAC 3 cut(s) 806, 1159, 1590
AccII CGCG 1 cut(s) 478
AccIII TCCGGA 1 cut(s) 2410
AclWI GGATC 6 cut(s) 329, 465, 597, 2155, 2168, 3399
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 6 cut(s) 174, 574, 1942, 2022, 2543, 3495
AcuI CTGAAG 2 cut(s) 171, 1027
AcyI GRCGYC 1 cut(s) 3515
AfaI GTAC 7 cut(s) 783, 1326, 2268, 2610, 2846, 2895, 3701
AfiI CCNNNNNNNGG 5 cut(s) 412, 1355, 1760, 2890, 3709
AflII CTTAAG 2 cut(s) 2120, 2330
AflIII ACRYGT 1 cut(s) 3213
AhdI GACNNNNNGTC 1 cut(s) 3878
AjiI CACGTC 1 cut(s) 862
AjnI CCWGG 5 cut(s) 428, 992, 2652, 2983, 3075
AjuI GAANNNNNNNTTGG 4 cut(s) 876, 908, 1704, 1736
AloI GAACNNNNNNTCC 4 cut(s) 1350, 1382, 2979, 3011
Alw21I GWGCWC 4 cut(s) 159, 270, 1384, 2606
Alw26I GTCTC 7 cut(s) 419, 516, 974, 1292, 3428, 3506, 3865
AlwI GGATC 6 cut(s) 329, 465, 597, 2155, 2168, 3399
AlwNI CAGNNNCTG 2 cut(s) 1111, 3348
Aor13HI TCCGGA 1 cut(s) 2410
AoxI GGCC 4 cut(s) 3, 3291, 3581, 3800
ApeKI GCWGC 7 cut(s) 301, 2136, 2388, 3348, 3406, 3810, 3813
ApoI RAATTY 6 cut(s) 174, 574, 1942, 2022, 2543, 3495
AseI ATTAAT 2 cut(s) 3084, 3645
Asp700I GAANNNNTTC 4 cut(s) 2196, 2406, 2547, 2861
AspLEI GCGC 3 cut(s) 1488, 2800, 3836
AspS9I GGNCC 7 cut(s) 2370, 2460, 2656, 3454, 3582, 3625, 3800
AsuC2I CCSGG 2 cut(s) 1202, 3791
AsuHPI GGTGA 2 cut(s) 643, 3695
AsuII TTCGAA 2 cut(s) 349, 1228
AvaII GGWCC 5 cut(s) 2370, 2460, 2656, 3454, 3625
BaeGI GKGCMC 1 cut(s) 318
BamHI GGATCC 1 cut(s) 2160
BanI GGYRCC 1 cut(s) 313
BanII GRGCYC 4 cut(s) 159, 270, 993, 3613
BarI GAAGNNNNNNTAC 2 cut(s) 38, 70
BbsI GAAGAC 1 cut(s) 46
Bbv12I GWGCWC 4 cut(s) 159, 270, 1384, 2606
BbvI GCAGC 7 cut(s) 313, 2123, 2375, 3335, 3418, 3797, 3800
BccI CCATC 8 cut(s) 108, 1754, 2071, 2240, 2378, 2450, 2782, 3021
BceAI ACGGC 2 cut(s) 1554, 1837
BcgI CGANNNNNNTGC 4 cut(s) 73, 107, 3654, 3688
BciT130I CCWGG 5 cut(s) 430, 994, 2654, 2985, 3077
BcnI CCSGG 2 cut(s) 1202, 3791
BcoDI GTCTC 7 cut(s) 419, 516, 974, 1292, 3428, 3506, 3865
BfrI CTTAAG 2 cut(s) 2120, 2330
BfuAI ACCTGC 1 cut(s) 2961
BglII AGATCT 1 cut(s) 2308
BisI GCNGC 9 cut(s) 6, 302, 2137, 2389, 3349, 3404, 3407, 3811, 3814
BlsI GCNGC 9 cut(s) 7, 303, 2138, 2390, 3350, 3405, 3408, 3812, 3815
BmcAI AGTACT 2 cut(s) 1326, 2610
Bme1390I CCNGG 7 cut(s) 430, 994, 1202, 2654, 2985, 3077, 3791
Bme18I GGWCC 5 cut(s) 2370, 2460, 2656, 3454, 3625
BmeRI GACNNNNNGTC 1 cut(s) 3878
BmgBI CACGTC 1 cut(s) 862
BmgT120I GGNCC 7 cut(s) 2370, 2460, 2656, 3454, 3582, 3625, 3800
BmiI GGNNCC 6 cut(s) 315, 2162, 2372, 2461, 3456, 3610
BmrFI CCNGG 7 cut(s) 430, 994, 1202, 2654, 2985, 3077, 3791
BmrI ACTGGG 2 cut(s) 1053, 2301
BmsI GCATC 5 cut(s) 1537, 1823, 2785, 3348, 3821
BmuI ACTGGG 2 cut(s) 1053, 2301
BpiI GAAGAC 1 cut(s) 46
BpmI CTGGAG 4 cut(s) 990, 1629, 1798, 3459
Bpu10I CCTNAGC 1 cut(s) 1104
Bpu14I TTCGAA 2 cut(s) 349, 1228
BpuEI CTTGAG 4 cut(s) 1788, 2036, 2297, 2377
BpuMI CCSGG 2 cut(s) 1202, 3791
Bsa29I ATCGAT 1 cut(s) 144
BsaBI GATNNNNATC 2 cut(s) 545, 1515
BsaHI GRCGYC 1 cut(s) 3515
BsaI GGTCTC 1 cut(s) 419
BsaJI CCNNGG 2 cut(s) 992, 3076
BsaWI WCCGGW 1 cut(s) 2410
BsaXI ACNNNNNCTCC 5 cut(s) 350, 380, 1602, 1632, 3862
Bsc4I CCNNNNNNNGG 5 cut(s) 412, 1355, 1760, 2890, 3709
Bse8I GATNNNNATC 2 cut(s) 545, 1515
BseAI TCCGGA 1 cut(s) 2410
BseBI CCWGG 5 cut(s) 430, 994, 2654, 2985, 3077
BseCI ATCGAT 1 cut(s) 144
BseDI CCNNGG 2 cut(s) 992, 3076
BseJI GATNNNNATC 2 cut(s) 545, 1515
BseLI CCNNNNNNNGG 5 cut(s) 412, 1355, 1760, 2890, 3709
BseMII CTCAG 8 cut(s) 73, 1118, 1992, 2616, 2619, 2855, 3219, 3279
BseRI GAGGAG 3 cut(s) 2473, 3764, 3767
BseSI GKGCMC 1 cut(s) 318
BseXI GCAGC 7 cut(s) 313, 2123, 2375, 3335, 3418, 3797, 3800
BseYI CCCAGC 2 cut(s) 468, 1478
BsgI GTGCAG 3 cut(s) 423, 848, 2689
Bsh1236I CGCG 1 cut(s) 478
BshFI GGCC 4 cut(s) 5, 3293, 3583, 3802
BshNI GGYRCC 1 cut(s) 313
BshVI ATCGAT 1 cut(s) 144
BsiHKAI GWGCWC 4 cut(s) 159, 270, 1384, 2606
BsiSI CCGG 4 cut(s) 1202, 2411, 3623, 3790
BslFI GGGAC 5 cut(s) 1257, 1422, 1799, 2473, 2651
BslI CCNNNNNNNGG 5 cut(s) 412, 1355, 1760, 2890, 3709
BsmAI GTCTC 7 cut(s) 419, 516, 974, 1292, 3428, 3506, 3865
BsmBI CGTCTC 1 cut(s) 3506
BsmFI GGGAC 5 cut(s) 1257, 1422, 1799, 2473, 2651
BsmI GAATGC 4 cut(s) 290, 1562, 2139, 2349
BsnI GGCC 4 cut(s) 5, 3293, 3583, 3802
Bso31I GGTCTC 1 cut(s) 419
Bsp119I TTCGAA 2 cut(s) 349, 1228
Bsp1286I GDGCHC 7 cut(s) 159, 270, 318, 993, 1384, 2606, 3613
Bsp13I TCCGGA 1 cut(s) 2410
Bsp68I TCGCGA 1 cut(s) 478
BspANI GGCC 4 cut(s) 5, 3293, 3583, 3802
BspCNI CTCAG 8 cut(s) 72, 1117, 1993, 2617, 2618, 2854, 3218, 3280
BspDI ATCGAT 1 cut(s) 144
BspEI TCCGGA 1 cut(s) 2410
BspFNI CGCG 1 cut(s) 478
BspHI TCATGA 1 cut(s) 2427
BspLI GGNNCC 6 cut(s) 315, 2162, 2372, 2461, 3456, 3610
BspMI ACCTGC 1 cut(s) 2961
BspPI GGATC 6 cut(s) 329, 465, 597, 2155, 2168, 3399
BspQI GCTCTTC 2 cut(s) 147, 2082
BspT104I TTCGAA 2 cut(s) 349, 1228
BspT107I GGYRCC 1 cut(s) 313
BspTI CTTAAG 2 cut(s) 2120, 2330
BspTNI GGTCTC 1 cut(s) 419
BssECI CCNNGG 2 cut(s) 992, 3076
BssNAI GTATAC 1 cut(s) 1160
BssNI GRCGYC 1 cut(s) 3515
Bst1107I GTATAC 1 cut(s) 1160
Bst2UI CCWGG 5 cut(s) 430, 994, 2654, 2985, 3077
Bst4CI ACNGT 6 cut(s) 483, 1334, 1970, 2824, 3428, 3785
Bst6I CTCTTC 6 cut(s) 147, 767, 2082, 2967, 2985, 3741
BstACI GRCGYC 1 cut(s) 3515
BstAFI CTTAAG 2 cut(s) 2120, 2330
BstAPI GCANNNNNTGC 1 cut(s) 1547
BstBI TTCGAA 2 cut(s) 349, 1228
BstC8I GCNNGC 4 cut(s) 1838, 2035, 2114, 2743
BstENI CCTNNNNNAGG 1 cut(s) 410
BstFNI CGCG 1 cut(s) 478
BstHHI GCGC 3 cut(s) 1488, 2800, 3836
BstMAI GTCTC 7 cut(s) 419, 516, 974, 1292, 3428, 3506, 3865
BstMWI GCNNNNNNNGC 7 cut(s) 566, 675, 1547, 2233, 2443, 3059, 3345
BstNI CCWGG 5 cut(s) 430, 994, 2654, 2985, 3077
BstNSI RCATGY 7 cut(s) 1040, 1287, 1420, 1894, 3217, 3403, 3700
BstSCI CCNGG 7 cut(s) 428, 992, 1200, 2652, 2983, 3075, 3789
BstSLI GKGCMC 1 cut(s) 318
BstUI CGCG 1 cut(s) 478
BstV1I GCAGC 7 cut(s) 313, 2123, 2375, 3335, 3418, 3797, 3800
BstV2I GAAGAC 1 cut(s) 46
BstX2I RGATCY 5 cut(s) 334, 457, 2160, 2308, 3391
BstXI CCANNNNNNTGG 1 cut(s) 577
BstYI RGATCY 5 cut(s) 334, 457, 2160, 2308, 3391
BstZ17I GTATAC 1 cut(s) 1160
Bsu15I ATCGAT 1 cut(s) 144
BsuRI GGCC 4 cut(s) 5, 3293, 3583, 3802
BsuTUI ATCGAT 1 cut(s) 144
BtgZI GCGATG 1 cut(s) 1264
BtrI CACGTC 1 cut(s) 862
BtsI GCAGTG 1 cut(s) 3777
BtsIMutI CAGTG 6 cut(s) 966, 1172, 2289, 3039, 3285, 3777
BtuMI TCGCGA 1 cut(s) 478
BveI ACCTGC 1 cut(s) 2961
Cac8I GCNNGC 4 cut(s) 1838, 2035, 2114, 2743
CaiI CAGNNNCTG 2 cut(s) 1111, 3348
CciI TCATGA 1 cut(s) 2427
CfoI GCGC 3 cut(s) 1488, 2800, 3836
Cfr13I GGNCC 7 cut(s) 2370, 2460, 2656, 3454, 3582, 3625, 3800
ClaI ATCGAT 1 cut(s) 144
Csp6I GTAC 7 cut(s) 782, 1325, 2267, 2609, 2845, 2894, 3700
CviQI GTAC 7 cut(s) 782, 1325, 2267, 2609, 2845, 2894, 3700
DrdI GACNNNNNNGTC 1 cut(s) 561
DriI GACNNNNNGTC 1 cut(s) 3878
DseDI GACNNNNNNGTC 1 cut(s) 561
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 6 cut(s) 147, 767, 2082, 2967, 2985, 3741
Eam1105I GACNNNNNGTC 1 cut(s) 3878
EarI CTCTTC 6 cut(s) 147, 767, 2082, 2967, 2985, 3741
Ecl136II GAGCTC 2 cut(s) 157, 268
Eco24I GRGCYC 4 cut(s) 159, 270, 993, 3613
Eco31I GGTCTC 1 cut(s) 419
Eco47I GGWCC 5 cut(s) 2370, 2460, 2656, 3454, 3625
Eco53kI GAGCTC 2 cut(s) 157, 268
Eco57I CTGAAG 2 cut(s) 171, 1027
EcoICRI GAGCTC 2 cut(s) 157, 268
EcoNI CCTNNNNNAGG 1 cut(s) 410
EcoO109I RGGNCCY 2 cut(s) 2370, 2656
EcoRII CCWGG 5 cut(s) 428, 992, 2652, 2983, 3075
EcoT22I ATGCAT 2 cut(s) 1422, 1601
EcoT38I GRGCYC 4 cut(s) 159, 270, 993, 3613
Esp3I CGTCTC 1 cut(s) 3506
FalI AAGNNNNNCTT 4 cut(s) 479, 511, 876, 908
FaqI GGGAC 5 cut(s) 1257, 1422, 1799, 2473, 2651
FauI CCCGC 3 cut(s) 565, 783, 1845
FauNDI CATATG 3 cut(s) 985, 2355, 3460
FblI GTMKAC 3 cut(s) 806, 1159, 1590
Fnu4HI GCNGC 9 cut(s) 6, 302, 2137, 2389, 3349, 3404, 3407, 3811, 3814
FriOI GRGCYC 4 cut(s) 159, 270, 993, 3613
Fsp4HI GCNGC 9 cut(s) 6, 302, 2137, 2389, 3349, 3404, 3407, 3811, 3814
FspAI RTGCGCAY 1 cut(s) 2799
FspI TGCGCA 1 cut(s) 2799
GlaI GCGC 3 cut(s) 1487, 2799, 3835
GluI GCNGC 9 cut(s) 6, 302, 2137, 2389, 3349, 3404, 3407, 3811, 3814
GsaI CCCAGC 2 cut(s) 472, 1482
GsuI CTGGAG 4 cut(s) 990, 1629, 1798, 3459
HaeIII GGCC 4 cut(s) 5, 3293, 3583, 3802
HapII CCGG 4 cut(s) 1202, 2411, 3623, 3790
HhaI GCGC 3 cut(s) 1488, 2800, 3836
Hin1I GRCGYC 1 cut(s) 3515
Hin6I GCGC 3 cut(s) 1486, 2798, 3834
HinP1I GCGC 3 cut(s) 1486, 2798, 3834
HincII GTYRAC 4 cut(s) 1375, 1591, 1857, 2721
HindII GTYRAC 4 cut(s) 1375, 1591, 1857, 2721
HindIII AAGCTT 4 cut(s) 375, 1234, 2739, 3571
HpaI GTTAAC 1 cut(s) 2721
HpaII CCGG 4 cut(s) 1202, 2411, 3623, 3790
HphI GGTGA 2 cut(s) 643, 3695
Hpy99I CGWCG 1 cut(s) 3676
HpyCH4III ACNGT 6 cut(s) 483, 1334, 1970, 2824, 3428, 3785
HpyCH4IV ACGT 3 cut(s) 861, 1905, 3515
HpyF10VI GCNNNNNNNGC 7 cut(s) 566, 675, 1547, 2233, 2443, 3059, 3345
HpySE526I ACGT 3 cut(s) 861, 1905, 3515
Hsp92I GRCGYC 1 cut(s) 3515
HspAI GCGC 3 cut(s) 1486, 2798, 3834
Kpn2I TCCGGA 1 cut(s) 2410
KspAI GTTAAC 1 cut(s) 2721
LguI GCTCTTC 2 cut(s) 147, 2082
LmnI GCTCC 5 cut(s) 1136, 1610, 2434, 3608, 3712
Lsp1109I GCAGC 7 cut(s) 313, 2123, 2375, 3335, 3418, 3797, 3800
LweI GCATC 5 cut(s) 1537, 1823, 2785, 3348, 3821
MaeII ACGT 3 cut(s) 861, 1905, 3515
MaeIII GTNAC 8 cut(s) 934, 964, 1315, 1901, 1964, 1981, 3521, 3717
MfeI CAATTG 1 cut(s) 2487
MflI RGATCY 5 cut(s) 334, 457, 2160, 2308, 3391
MhlI GDGCHC 7 cut(s) 159, 270, 318, 993, 1384, 2606, 3613
MlyI GAGTC 6 cut(s) 1404, 1522, 1959, 3571, 3677, 3686
MmeI TCCRAC 3 cut(s) 631, 2911, 3654
Mph1103I ATGCAT 2 cut(s) 1422, 1601
MroI TCCGGA 1 cut(s) 2410
MroXI GAANNNNTTC 4 cut(s) 2196, 2406, 2547, 2861
MslI CAYNNNNRTG 2 cut(s) 2354, 2454
MspCI CTTAAG 2 cut(s) 2120, 2330
MspI CCGG 4 cut(s) 1202, 2411, 3623, 3790
MspR9I CCNGG 7 cut(s) 430, 994, 1202, 2654, 2985, 3077, 3791
MunI CAATTG 1 cut(s) 2487
Mva1269I GAATGC 4 cut(s) 290, 1562, 2139, 2349
MvaI CCWGG 5 cut(s) 430, 994, 2654, 2985, 3077
MvnI CGCG 1 cut(s) 478
MwoI GCNNNNNNNGC 7 cut(s) 566, 675, 1547, 2233, 2443, 3059, 3345
NciI CCSGG 2 cut(s) 1202, 3791
NdeI CATATG 3 cut(s) 985, 2355, 3460
NlaIV GGNNCC 6 cut(s) 315, 2162, 2372, 2461, 3456, 3610
NmeAIII GCCGAG 1 cut(s) 3559
NmuCI GTSAC 3 cut(s) 964, 1901, 1981
NruI TCGCGA 1 cut(s) 478
NsbI TGCGCA 1 cut(s) 2799
NsiI ATGCAT 2 cut(s) 1422, 1601
NspI RCATGY 7 cut(s) 1040, 1287, 1420, 1894, 3217, 3403, 3700
NspV TTCGAA 2 cut(s) 349, 1228
PagI TCATGA 1 cut(s) 2427
PciI ACATGT 1 cut(s) 3213
PciSI GCTCTTC 2 cut(s) 147, 2082
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 4 cut(s) 290, 1562, 2139, 2349
PdmI GAANNNNTTC 4 cut(s) 2196, 2406, 2547, 2861
PflMI CCANNNNNTGG 1 cut(s) 3709
PfoI TCCNGGA 2 cut(s) 1200, 2983
PkrI GCNGC 9 cut(s) 7, 303, 2138, 2390, 3350, 3405, 3408, 3812, 3815
PleI GAGTC 6 cut(s) 1404, 1522, 1958, 3571, 3676, 3685
PpsI GAGTC 6 cut(s) 1404, 1522, 1958, 3571, 3676, 3685
PpuMI RGGWCCY 2 cut(s) 2370, 2656
PscI ACATGT 1 cut(s) 3213
PshBI ATTAAT 2 cut(s) 3084, 3645
PsiI TTATAA 1 cut(s) 747
Psp124BI GAGCTC 2 cut(s) 159, 270
Psp5II RGGWCCY 2 cut(s) 2370, 2656
Psp6I CCWGG 5 cut(s) 428, 992, 2652, 2983, 3075
PspFI CCCAGC 2 cut(s) 468, 1478
PspGI CCWGG 5 cut(s) 428, 992, 2652, 2983, 3075
PspN4I GGNNCC 6 cut(s) 315, 2162, 2372, 2461, 3456, 3610
PspPI GGNCC 7 cut(s) 2370, 2460, 2656, 3454, 3582, 3625, 3800
PspPPI RGGWCCY 2 cut(s) 2370, 2656
PstNI CAGNNNCTG 2 cut(s) 1111, 3348
PsuI RGATCY 5 cut(s) 334, 457, 2160, 2308, 3391
RruI TCGCGA 1 cut(s) 478
RsaI GTAC 7 cut(s) 783, 1326, 2268, 2610, 2846, 2895, 3701
RsaNI GTAC 7 cut(s) 782, 1325, 2267, 2609, 2845, 2894, 3700
RseI CAYNNNNRTG 2 cut(s) 2354, 2454
SacI GAGCTC 2 cut(s) 159, 270
SalI GTCGAC 1 cut(s) 1589
SapI GCTCTTC 2 cut(s) 147, 2082
SatI GCNGC 9 cut(s) 6, 302, 2137, 2389, 3349, 3404, 3407, 3811, 3814
Sau96I GGNCC 7 cut(s) 2370, 2460, 2656, 3454, 3582, 3625, 3800
ScaI AGTACT 2 cut(s) 1326, 2610
SchI GAGTC 6 cut(s) 1404, 1522, 1959, 3571, 3677, 3686
ScrFI CCNGG 7 cut(s) 430, 994, 1202, 2654, 2985, 3077, 3791
SduI GDGCHC 7 cut(s) 159, 270, 318, 993, 1384, 2606, 3613
SfaNI GCATC 5 cut(s) 1537, 1823, 2785, 3348, 3821
SfuI TTCGAA 2 cut(s) 349, 1228
SinI GGWCC 5 cut(s) 2370, 2460, 2656, 3454, 3625
SmiMI CAYNNNNRTG 2 cut(s) 2354, 2454
SmlI CTYRAG 6 cut(s) 1803, 2015, 2120, 2312, 2330, 2392
SmoI CTYRAG 6 cut(s) 1803, 2015, 2120, 2312, 2330, 2392
SstI GAGCTC 2 cut(s) 159, 270
StyD4I CCNGG 7 cut(s) 428, 992, 1200, 2652, 2983, 3075, 3789
TaaI ACNGT 6 cut(s) 483, 1334, 1970, 2824, 3428, 3785
TaiI ACGT 3 cut(s) 864, 1908, 3518
TatI WGTACW 3 cut(s) 781, 1324, 2608
TauI GCSGC 2 cut(s) 8, 3406
TscAI CASTG 6 cut(s) 973, 1172, 2296, 3039, 3292, 3777
TseFI GTSAC 3 cut(s) 964, 1901, 1981
TseI GCWGC 7 cut(s) 301, 2136, 2388, 3348, 3406, 3810, 3813
Tsp45I GTSAC 3 cut(s) 964, 1901, 1981
TspGWI ACGGA 4 cut(s) 470, 553, 1559, 3540
TspRI CASTG 6 cut(s) 973, 1172, 2296, 3039, 3292, 3777
Van91I CCANNNNNTGG 1 cut(s) 3709
Vha464I CTTAAG 2 cut(s) 2120, 2330
VpaK11BI GGWCC 5 cut(s) 2370, 2460, 2656, 3454, 3625
VspI ATTAAT 2 cut(s) 3084, 3645
XagI CCTNNNNNAGG 1 cut(s) 410
XapI RAATTY 6 cut(s) 174, 574, 1942, 2022, 2543, 3495
XbaI TCTAGA 2 cut(s) 115, 1273
XceI RCATGY 7 cut(s) 1040, 1287, 1420, 1894, 3217, 3403, 3700
XcmI CCANNNNNNNNNTGG 1 cut(s) 3020
XmiI GTMKAC 3 cut(s) 806, 1159, 1590
XmnI GAANNNNTTC 4 cut(s) 2196, 2406, 2547, 2861
ZraI GACGTC 1 cut(s) 3516
ZrmI AGTACT 2 cut(s) 1326, 2610
Zsp2I ATGCAT 2 cut(s) 1422, 1601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.