Rroxscaffold_5G00352760

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000067
Physical Location & Seq
Reverse (-)
30021008 .. 30027617
6610 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00352760.1

Sequence Viewer

Length: 2199 bp
ATGGAATATGCTCAAGGTTTGCCCTTAGCGCTTAAAGTCTTGGGGGCTTTTCTTGATAACAAAAGTGTGTGTGAATGGGAAGCCGTGTTGGTGAAATTGAAGAAAACCCCACAAATAGAGCTTCAGGATGTGCTTAGAACAAGCTTTGATGAACTAGACGATTCAGAGAAGGACATATTTCTAGATATTGCATGTCTCTTTAGAGGATGGAGCAAAAGTTCTGTCACAGAATTATTAGACAGTTTTGGCTTCTTTCCCCACAATGGATTAAGAGTTCTAATTGACAGAGCTCTCATAACTATATCACGTTTCGATCAAGTCGACATGCATGATTTAATACAAGAAATGGGTAGAGAAATCGTCCGCCTAGAATCTACCAAAGAACCTGGGATGCGCAGTAGGTTGTGGAGTAGTGAAGATGCTAATTGTGTATTGACTCAAAAAAGGGCTACTGATGCAGTCGAATGCTTAGTGCTAAAGTTGTCGAACTCAAAAGTTGACCTATGCATAGATACTAAGGCCTTTGTAAGAATGACGAAGCTAAGACTACTCATAATCTATTATTCAATCCCTGCAGCGTTCAATTTTGCCTACTGCTGGCCACACAAGAGCATATGCCCAGATGGTGGCCGTAAACAGCTCACGAGGGGAGACTTCGAGTTTCTTTCTCATGAGTTGAGGTGTCTCTTATGGCATGGATGCCCGCTAAAGTCTTTGCCATCAACCTATATCCCAAAGAATCTTGTTAAACTTGATATGCGTGGTAGTCACATTCGAGAACTTTGGGAAGGAATCAAGCCCCTACAAAATCTGAAAGTCATCACGTTAAGTCACTCTCAATACCTTGTCAAAATCCCTGACTTGACAGAGGCGGTAAATCTCGAGAAACTACTTCTTAATGATTGTTCAAGTTTACTTGAGGTCCATCCATCCATTTCTGCTCTTCAAAACCTTGATTTCTTGAATTTAAAGCGGTGCAAAGGACTCAACAATCTCCCAAGCAGCATTCGTATGAAATCTCTAAAAACCCTTGATATTATTGGCTGTTCAAATCTTCAAAAGTTTCCAGAGATTCCAGATGTTATGAAGGAGCTAACAGATCTTTGCTTAGATGGGACTGCAATTAAAGAACTGCCCTCATCGATTAGTAATCTTACAGGCCTTGTTACTTTGGACCTCAGTGGTTGCAGAGAATTTAAGAGTTTGCCTGGCAGCATTCATATGAAATCTCTGAAAACCCTTAATCTTTCTGGCTGTTCAAATGTGGATAAGTTTCCAGAGATTCCAGAGGTTATGACGGAGCTCAAGGAGGTTTATCTAGATGAGACTGCAATAAAAGAACTACCCTCATCAATTGAACGTCTTATGGGGATTAAAATTTTAAGTATGCGGAACTGCAAAAGCATTGAATTTCTTCCCGACAGTATCTGTAATTTGGCGGACCTTACACATCTCAATCTGTCTGGGTGCTCAGGACTTCATAACTTGCCTGAGAAATTGGGAGACTTAGAATCCTTACAGGTCCTTGACGTAAGTTATAGTGGTATAAACCGTCTTCCATTTTCTATCCTTTATGTGGGCGACACATTATCATGTATTGGATGTAAAGAGATGACGGCACCCTTGTCAGCATTGCCATCATCAATGGAAGAATTCTGGAGTTACTCTGTTCTGCTACATCTTGATTTAAGTGACTGCAGTCTGTCGGAGTTATCAGATGGTATTGCTGATTTGTCTTCACTGAAAACATTAAAGCTCTGCAGAAACAATTTGGAGAGCTTACCTGTGACAATTAATCGACTTGGTTGTTTAACGCATCTTGAATTGGAAGCTTGCAAGAGGCTGAAATCAATACCAGAGCTTTCATCGAGTATAAATTACATAGATGCACATGATTGCATAGCTTTGGAAACAGTTTCAAAGCCAAAGCCTCAGTACCAGACGGATCATTTCTTTACATTTTCTAATTGCCTCCAGCTGGTACAAACAAATCTATTTACAGATATTGTGGAAAGACATTCACATTTGCAGGGTAGTCGTAGTTACCTGGGACCTCTTCAGTTAAATATGTCTCTTCCTGGGAGTGAAGTTTCTGATTGGTTCACTCATCAGAGTAGGGGAGTTTCAGTGAGTGCGCAGCTCCCACCAAATTGGTTTGACAGTAAGTTTTTTGGGATTCGCTATCTGCGCTGTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

732

Amino Acids

82.27

Weight (kDa)

5.89

Isoelectric Point (pI)

39.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 55 - 124 2.4e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 330 - 437 7.4e-08 Leucine-rich repeat region
LRR_13 PF23286 339 - 408 2e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 408 - 494 2.3e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 410 - 491 3.2e-07 Leucine-rich repeat region
LRR_14 PF23598 548 - 621 3.7e-08 Leucine-rich repeat region
LRR_8 PF13855 560 - 612 3.1e-06 Leucine rich repeat
C-JID PF20160 692 - 726 2.1e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 395, 2136
AccB1I GGYRCC 1 cut(s) 1618
AccB7I CCANNNNNTGG 1 cut(s) 626
AccI GTMKAC 1 cut(s) 321
AciI CCGC 6 cut(s) 364, 704, 872, 973, 1390, 1439
AclWI GGATC 1 cut(s) 1953
AcoI YGGCCR 2 cut(s) 599, 628
AcsI RAATTY 5 cut(s) 964, 1193, 1377, 1409, 1652
AcuI CTGAAG 2 cut(s) 107, 2042
AfaI GTAC 2 cut(s) 1937, 1983
AfeI AGCGCT 1 cut(s) 30
AfiI CCNNNNNNNGG 5 cut(s) 263, 597, 626, 1576, 1978
AjnI CCWGG 4 cut(s) 385, 1207, 2046, 2077
AloI GAACNNNNNNTCC 4 cut(s) 202, 234, 258, 290
Alw21I GWGCWC 3 cut(s) 292, 1305, 1472
Alw26I GTCTC 6 cut(s) 200, 645, 689, 1319, 1497, 2076
AlwI GGATC 1 cut(s) 1953
AlwNI CAGNNNCTG 1 cut(s) 1428
Ama87I CYCGRG 1 cut(s) 881
Aor51HI AGCGCT 1 cut(s) 30
AoxI GGCC 4 cut(s) 519, 599, 628, 1159
ApeKI GCWGC 4 cut(s) 575, 1002, 1212, 2137
ApoI RAATTY 5 cut(s) 964, 1193, 1377, 1409, 1652
ArsI GACNNNNNNTTYG 2 cut(s) 207, 239
AseI ATTAAT 1 cut(s) 1794
Asp700I GAANNNNTTC 1 cut(s) 1413
AspLEI GCGC 4 cut(s) 31, 396, 2137, 2190
AspS9I GGNCC 5 cut(s) 922, 1174, 1441, 1522, 2051
AsuHPI GGTGA 1 cut(s) 103
AvaI CYCGRG 1 cut(s) 881
AvaII GGWCC 5 cut(s) 922, 1174, 1441, 1522, 2051
BalI TGGCCA 1 cut(s) 601
BanI GGYRCC 1 cut(s) 1618
BanII GRGCYC 2 cut(s) 292, 1305
BauI CACGAG 1 cut(s) 643
BbsI GAAGAC 2 cut(s) 1547, 1728
Bbv12I GWGCWC 3 cut(s) 292, 1305, 1472
BbvI GCAGC 4 cut(s) 587, 1014, 1224, 2149
BccI CCATC 8 cut(s) 201, 617, 727, 933, 937, 1106, 1645, 1712
BceAI ACGGC 3 cut(s) 68, 615, 1632
BcgI CGANNNNNNTGC 2 cut(s) 2018, 2052
BciT130I CCWGG 4 cut(s) 387, 1209, 2048, 2079
BcoDI GTCTC 6 cut(s) 200, 645, 689, 1319, 1497, 2076
BfaI CTAG 4 cut(s) 155, 182, 368, 1319
BfmI CTRYAG 3 cut(s) 573, 1696, 1759
BfoI RGCGCY 1 cut(s) 32
BglII AGATCT 1 cut(s) 1099
BisI GCNGC 4 cut(s) 576, 1003, 1213, 2138
BlsI GCNGC 4 cut(s) 577, 1004, 1214, 2139
Bme1390I CCNGG 4 cut(s) 387, 1209, 2048, 2079
Bme18I GGWCC 5 cut(s) 922, 1174, 1441, 1522, 2051
BmeT110I CYCGRG 1 cut(s) 881
BmgT120I GGNCC 5 cut(s) 922, 1174, 1441, 1522, 2051
BmiI GGNNCC 2 cut(s) 1620, 2052
BmrFI CCNGG 4 cut(s) 387, 1209, 2048, 2079
BmsI GCATC 6 cut(s) 381, 409, 445, 689, 1825, 1876
BoxI GACNNNNGTC 1 cut(s) 1698
BpiI GAAGAC 2 cut(s) 1547, 1728
BpmI CTGGAG 2 cut(s) 1678, 1958
Bpu10I CCTNAGC 2 cut(s) 25, 1471
BpuEI CTTGAG 2 cut(s) 938, 1289
Bsa29I ATCGAT 1 cut(s) 1142
BsaJI CCNNGG 3 cut(s) 386, 2047, 2078
BsaXI ACNNNNNCTCC 4 cut(s) 202, 232, 2112, 2142
Bsc4I CCNNNNNNNGG 5 cut(s) 263, 597, 626, 1576, 1978
Bse3DI GCAATG 1 cut(s) 1631
BseBI CCWGG 4 cut(s) 387, 1209, 2048, 2079
BseCI ATCGAT 1 cut(s) 1142
BseDI CCNNGG 3 cut(s) 386, 2047, 2078
BseGI GGATG 7 cut(s) 133, 212, 396, 704, 925, 929, 1607
BseLI CCNNNNNNNGG 5 cut(s) 263, 597, 626, 1576, 1978
BseMI GCAATG 1 cut(s) 1631
BseMII CTCAG 4 cut(s) 1192, 1482, 1485, 1946
BseXI GCAGC 4 cut(s) 587, 1014, 1224, 2149
BshFI GGCC 4 cut(s) 521, 601, 630, 1161
BshNI GGYRCC 1 cut(s) 1618
BshVI ATCGAT 1 cut(s) 1142
BsiHKAI GWGCWC 3 cut(s) 292, 1305, 1472
BsiHKCI CYCGRG 1 cut(s) 881
BslFI GGGAC 2 cut(s) 1129, 2064
BslI CCNNNNNNNGG 5 cut(s) 263, 597, 626, 1576, 1978
BsmAI GTCTC 6 cut(s) 200, 645, 689, 1319, 1497, 2076
BsmFI GGGAC 2 cut(s) 1129, 2064
BsmI GAATGC 3 cut(s) 470, 1005, 1215
BsnI GGCC 4 cut(s) 521, 601, 630, 1161
BsoBI CYCGRG 1 cut(s) 881
Bsp1286I GDGCHC 3 cut(s) 292, 1305, 1472
Bsp143I GATC 3 cut(s) 313, 1099, 1945
BspACI CCGC 6 cut(s) 364, 704, 872, 973, 1390, 1439
BspANI GGCC 4 cut(s) 521, 601, 630, 1161
BspCNI CTCAG 4 cut(s) 1191, 1483, 1484, 1945
BspDI ATCGAT 1 cut(s) 1142
BspHI TCATGA 1 cut(s) 670
BspLI GGNNCC 2 cut(s) 1620, 2052
BspMAI CTGCAG 3 cut(s) 577, 1700, 1763
BspPI GGATC 1 cut(s) 1953
BspQI GCTCTTC 1 cut(s) 948
BspT107I GGYRCC 1 cut(s) 1618
BsrDI GCAATG 1 cut(s) 1631
BssECI CCNNGG 3 cut(s) 386, 2047, 2078
BssMI GATC 3 cut(s) 313, 1099, 1945
BssSI CACGAG 1 cut(s) 643
Bst2BI CACGAG 1 cut(s) 643
Bst2UI CCWGG 4 cut(s) 387, 1209, 2048, 2079
Bst4CI ACNGT 5 cut(s) 242, 1424, 1553, 1915, 2162
Bst6I CTCTTC 3 cut(s) 948, 2061, 2079
BstC8I GCNNGC 3 cut(s) 599, 704, 1834
BstF5I GGATG 7 cut(s) 133, 212, 396, 704, 925, 929, 1607
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 4 cut(s) 31, 396, 2137, 2190
BstKTI GATC 3 cut(s) 316, 1102, 1948
BstMAI GTCTC 6 cut(s) 200, 645, 689, 1319, 1497, 2076
BstMBI GATC 3 cut(s) 313, 1099, 1945
BstMWI GCNNNNNNNGC 3 cut(s) 28, 455, 2187
BstNI CCWGG 4 cut(s) 387, 1209, 2048, 2079
BstNSI RCATGY 2 cut(s) 195, 328
BstPAI GACNNNNGTC 1 cut(s) 1698
BstSCI CCNGG 4 cut(s) 385, 1207, 2046, 2077
BstSFI CTRYAG 3 cut(s) 573, 1696, 1759
BstV1I GCAGC 4 cut(s) 587, 1014, 1224, 2149
BstV2I GAAGAC 2 cut(s) 1547, 1728
BstX2I RGATCY 1 cut(s) 1099
BstXI CCANNNNNNTGG 1 cut(s) 2151
BstYI RGATCY 1 cut(s) 1099
Bsu15I ATCGAT 1 cut(s) 1142
BsuRI GGCC 4 cut(s) 521, 601, 630, 1161
BsuTUI ATCGAT 1 cut(s) 1142
BtsCI GGATG 7 cut(s) 133, 212, 396, 704, 925, 929, 1607
BtsIMutI CAGTG 3 cut(s) 1186, 1739, 2133
Cac8I GCNNGC 3 cut(s) 599, 704, 1834
CaiI CAGNNNCTG 1 cut(s) 1428
CciI TCATGA 1 cut(s) 670
CfoI GCGC 4 cut(s) 31, 396, 2137, 2190
Cfr13I GGNCC 5 cut(s) 922, 1174, 1441, 1522, 2051
ClaI ATCGAT 1 cut(s) 1142
Csp6I GTAC 2 cut(s) 1936, 1982
CviAII CATG 7 cut(s) 192, 325, 329, 671, 695, 1593, 1892
CviQI GTAC 2 cut(s) 1936, 1982
DpnI GATC 3 cut(s) 315, 1101, 1947
DpnII GATC 3 cut(s) 313, 1099, 1945
DraI TTTAAA 1 cut(s) 969
EaeI YGGCCR 2 cut(s) 599, 628
Eam1104I CTCTTC 3 cut(s) 948, 2061, 2079
EarI CTCTTC 3 cut(s) 948, 2061, 2079
EciI GGCGGA 2 cut(s) 353, 1454
Ecl136II GAGCTC 2 cut(s) 290, 1303
Eco147I AGGCCT 2 cut(s) 521, 1161
Eco24I GRGCYC 2 cut(s) 292, 1305
Eco47I GGWCC 5 cut(s) 922, 1174, 1441, 1522, 2051
Eco47III AGCGCT 1 cut(s) 30
Eco53kI GAGCTC 2 cut(s) 290, 1303
Eco57I CTGAAG 2 cut(s) 107, 2042
Eco88I CYCGRG 1 cut(s) 881
EcoICRI GAGCTC 2 cut(s) 290, 1303
EcoO109I RGGNCCY 2 cut(s) 1522, 2051
EcoRI GAATTC 1 cut(s) 1652
EcoRII CCWGG 4 cut(s) 385, 1207, 2046, 2077
EcoT22I ATGCAT 2 cut(s) 330, 509
EcoT38I GRGCYC 2 cut(s) 292, 1305
FaeI CATG 7 cut(s) 195, 328, 332, 674, 698, 1596, 1895
FaqI GGGAC 2 cut(s) 1129, 2064
FatI CATG 7 cut(s) 191, 324, 328, 670, 694, 1592, 1891
FauI CCCGC 1 cut(s) 711
FauNDI CATATG 2 cut(s) 614, 1221
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 4 cut(s) 576, 1003, 1213, 2138
FokI GGATG 7 cut(s) 140, 219, 403, 711, 912, 916, 1614
FriOI GRGCYC 2 cut(s) 292, 1305
Fsp4HI GCNGC 4 cut(s) 576, 1003, 1213, 2138
FspBI CTAG 4 cut(s) 155, 182, 368, 1319
FspI TGCGCA 2 cut(s) 395, 2136
GlaI GCGC 4 cut(s) 30, 395, 2136, 2189
GluI GCNGC 4 cut(s) 576, 1003, 1213, 2138
GsuI CTGGAG 2 cut(s) 1678, 1958
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 4 cut(s) 521, 601, 630, 1161
HhaI GCGC 4 cut(s) 31, 396, 2137, 2190
Hin1II CATG 7 cut(s) 195, 328, 332, 674, 698, 1596, 1895
Hin6I GCGC 4 cut(s) 29, 394, 2135, 2188
HinP1I GCGC 4 cut(s) 29, 394, 2135, 2188
HincII GTYRAC 2 cut(s) 322, 499
HindII GTYRAC 2 cut(s) 322, 499
HindIII AAGCTT 2 cut(s) 142, 1830
HphI GGTGA 1 cut(s) 103
Hpy166II GTNNAC 5 cut(s) 322, 499, 635, 914, 2103
Hpy188I TCNGA 7 cut(s) 166, 813, 1233, 1708, 1717, 2095, 2112
Hpy8I GTNNAC 5 cut(s) 322, 499, 635, 914, 2103
HpyAV CCTTC 3 cut(s) 163, 782, 1081
HpyCH4III ACNGT 5 cut(s) 242, 1424, 1553, 1915, 2162
HpyCH4IV ACGT 4 cut(s) 307, 824, 1360, 1530
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 455, 2187
HpySE526I ACGT 4 cut(s) 307, 824, 1360, 1530
Hsp92II CATG 7 cut(s) 195, 328, 332, 674, 698, 1596, 1895
HspAI GCGC 4 cut(s) 29, 394, 2135, 2188
Kzo9I GATC 3 cut(s) 313, 1099, 1945
LguI GCTCTTC 1 cut(s) 948
LmnI GCTCC 4 cut(s) 210, 1090, 1300, 2145
Lsp1109I GCAGC 4 cut(s) 587, 1014, 1224, 2149
LweI GCATC 6 cut(s) 381, 409, 445, 689, 1825, 1876
MaeI CTAG 4 cut(s) 155, 182, 368, 1319
MaeII ACGT 4 cut(s) 307, 824, 1360, 1530
MaeIII GTNAC 8 cut(s) 223, 767, 830, 1165, 1661, 1691, 1786, 2042
MalI GATC 3 cut(s) 315, 1101, 1947
MboI GATC 3 cut(s) 313, 1099, 1945
MfeI CAATTG 1 cut(s) 1353
MflI RGATCY 1 cut(s) 1099
MhlI GDGCHC 3 cut(s) 292, 1305, 1472
MlsI TGGCCA 1 cut(s) 601
MluNI TGGCCA 1 cut(s) 601
MlyI GAGTC 2 cut(s) 430, 978
MmeI TCCRAC 1 cut(s) 1686
Mox20I TGGCCA 1 cut(s) 601
Mph1103I ATGCAT 2 cut(s) 330, 509
MroXI GAANNNNTTC 1 cut(s) 1413
MscI TGGCCA 1 cut(s) 601
MslI CAYNNNNRTG 3 cut(s) 1010, 1220, 1591
Msp20I TGGCCA 1 cut(s) 601
MspA1I CMGCKG 1 cut(s) 1978
MspR9I CCNGG 4 cut(s) 387, 1209, 2048, 2079
MunI CAATTG 1 cut(s) 1353
Mva1269I GAATGC 3 cut(s) 470, 1005, 1215
MvaI CCWGG 4 cut(s) 387, 1209, 2048, 2079
MwoI GCNNNNNNNGC 3 cut(s) 28, 455, 2187
NdeI CATATG 2 cut(s) 614, 1221
NdeII GATC 3 cut(s) 313, 1099, 1945
NlaIII CATG 7 cut(s) 195, 328, 332, 674, 698, 1596, 1895
NlaIV GGNNCC 2 cut(s) 1620, 2052
NmuCI GTSAC 5 cut(s) 223, 767, 830, 1691, 1786
NsbI TGCGCA 2 cut(s) 395, 2136
NsiI ATGCAT 2 cut(s) 330, 509
NspI RCATGY 2 cut(s) 195, 328
PaeR7I CTCGAG 1 cut(s) 881
PagI TCATGA 1 cut(s) 670
PceI AGGCCT 2 cut(s) 521, 1161
PciSI GCTCTTC 1 cut(s) 948
PcsI WCGNNNNNNNCGW 1 cut(s) 318
PctI GAATGC 3 cut(s) 470, 1005, 1215
PdmI GAANNNNTTC 1 cut(s) 1413
PfeI GAWTC 8 cut(s) 161, 371, 739, 792, 1072, 1282, 1511, 2176
PflMI CCANNNNNTGG 1 cut(s) 626
PkrI GCNGC 4 cut(s) 577, 1004, 1214, 2139
PleI GAGTC 2 cut(s) 430, 978
PpsI GAGTC 2 cut(s) 430, 978
PpuMI RGGWCCY 2 cut(s) 1522, 2051
PshAI GACNNNNGTC 1 cut(s) 1698
PshBI ATTAAT 1 cut(s) 1794
Psp124BI GAGCTC 2 cut(s) 292, 1305
Psp5II RGGWCCY 2 cut(s) 1522, 2051
Psp6I CCWGG 4 cut(s) 385, 1207, 2046, 2077
PspGI CCWGG 4 cut(s) 385, 1207, 2046, 2077
PspN4I GGNNCC 2 cut(s) 1620, 2052
PspPI GGNCC 5 cut(s) 922, 1174, 1441, 1522, 2051
PspPPI RGGWCCY 2 cut(s) 1522, 2051
PstI CTGCAG 3 cut(s) 577, 1700, 1763
PstNI CAGNNNCTG 1 cut(s) 1428
PsuI RGATCY 1 cut(s) 1099
PvuII CAGCTG 1 cut(s) 1978
RsaI GTAC 2 cut(s) 1937, 1983
RsaNI GTAC 2 cut(s) 1936, 1982
RseI CAYNNNNRTG 3 cut(s) 1010, 1220, 1591
SacI GAGCTC 2 cut(s) 292, 1305
SalI GTCGAC 1 cut(s) 320
SapI GCTCTTC 1 cut(s) 948
SatI GCNGC 4 cut(s) 576, 1003, 1213, 2138
Sau3AI GATC 3 cut(s) 313, 1099, 1945
Sau96I GGNCC 5 cut(s) 922, 1174, 1441, 1522, 2051
SchI GAGTC 2 cut(s) 430, 978
ScrFI CCNGG 4 cut(s) 387, 1209, 2048, 2079
SduI GDGCHC 3 cut(s) 292, 1305, 1472
SfaNI GCATC 6 cut(s) 381, 409, 445, 689, 1825, 1876
SfcI CTRYAG 3 cut(s) 573, 1696, 1759
Sfr274I CTCGAG 1 cut(s) 881
SinI GGWCC 5 cut(s) 922, 1174, 1441, 1522, 2051
SlaI CTCGAG 1 cut(s) 881
SmiMI CAYNNNNRTG 3 cut(s) 1010, 1220, 1591
SmlI CTYRAG 4 cut(s) 12, 881, 917, 1304
SmoI CTYRAG 4 cut(s) 12, 881, 917, 1304
SseBI AGGCCT 2 cut(s) 521, 1161
SsiI CCGC 6 cut(s) 364, 704, 872, 973, 1390, 1439
SspMI CTAG 4 cut(s) 155, 182, 368, 1319
SstI GAGCTC 2 cut(s) 292, 1305
StuI AGGCCT 2 cut(s) 521, 1161
StyD4I CCNGG 4 cut(s) 385, 1207, 2046, 2077
TaaI ACNGT 5 cut(s) 242, 1424, 1553, 1915, 2162
TaiI ACGT 4 cut(s) 310, 827, 1363, 1533
TfiI GAWTC 8 cut(s) 161, 371, 739, 792, 1072, 1282, 1511, 2176
TscAI CASTG 3 cut(s) 1186, 1746, 2133
TseFI GTSAC 5 cut(s) 223, 767, 830, 1691, 1786
TseI GCWGC 4 cut(s) 575, 1002, 1212, 2137
Tsp45I GTSAC 5 cut(s) 223, 767, 830, 1691, 1786
TspDTI ATGAA 7 cut(s) 165, 1028, 1100, 1208, 1238, 1469, 1854
TspGWI ACGGA 2 cut(s) 1313, 1958
TspRI CASTG 3 cut(s) 1186, 1746, 2133
Van91I CCANNNNNTGG 1 cut(s) 626
VpaK11BI GGWCC 5 cut(s) 922, 1174, 1441, 1522, 2051
VspI ATTAAT 1 cut(s) 1794
XapI RAATTY 5 cut(s) 964, 1193, 1377, 1409, 1652
XbaI TCTAGA 2 cut(s) 181, 1318
XceI RCATGY 2 cut(s) 195, 328
XhoI CTCGAG 1 cut(s) 881
XmiI GTMKAC 1 cut(s) 321
XmnI GAANNNNTTC 1 cut(s) 1413
XspI CTAG 4 cut(s) 155, 182, 368, 1319
Zsp2I ATGCAT 2 cut(s) 330, 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.