MD04G1200400.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000067
Physical Location & Seq
Forward (+)
28697858 .. 28703385
5528 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1200400.v1.1.491

Sequence Viewer

Length: 3744 bp
ATGCTCGATCGATTAATGGCTGCTGCTTCCTCTTCTTCTTCTTCCTCTGTCCCTCGTTGGAAATACGACGTGTTCATCAATTTCAGGGGGGAAGACACTCGTAGGGGCTTCGTCAGCCACCTCTACAAAGCTCTGAATCAGAAATTTATCAACACCTATATCGATGCCGAAGAGCTCCGAAAAGGCAACGACCTTTCGCAGCTACTGACGGCGATCCGAGACTCGAGCGTTTCGATCGTAGTTTTTTCTCAAAACTACGCATCTTCCACATGGTGCTTGAAGGAGCTCGTCCAAATACTGGAATGTATGGATGTAAAGAACCAGATGGTGGTGCCCGTTTTTTACCAAGTGGATCCTTCTCATGTCCGCAAAATGAAGAGGCGTTTCGCGGAAGCTTTTGCCGAGCATGAAAGCGATCCTAATGCCGCCATGAGAGAGGTGCGGAGCTGGAGGTACGCTCTGAAAAGAGCCACCAATTTATCCGGTTGGGATTCGCGAAAATACGAGGATGATGCGAAGCTTATTGACGGAATTGTAGAAGATATTTTTAATAAGTTGATTCAGATGTCATCAAGTGAAGCTGATGATGGCTTGGTTGGAATGGATTCCCACATTCAGGAAATGGATATAAGATTAAGGCCTTGGGTAAAGGATGATGTTCGTGTCGTTGGAATATGGGGTATGGGTGGTTTAGGCAAAACAACCATCGCTAGAGCTGTTTATGATAAAATCGCTTGTCAATTTGAAGCAGATTGCGTTCTTGAAAATGTCAAGGAAGGTTTCTCCAAGAATGGGGCAGTGCATATGCAGGAATTGCTTCTATCTAGAATCTTGAAGGAGAAAGTGCAGAGGTTAGGCACTTTGGATCGAGGTTCGAATATGATAATGGAGAGGCTAGGTAATAAAAAGGTTCTTCTTGTTTTAGATGATGTGGACAATTTGGCTCAAATTGAAACCTTGCTGGGGAGAAATTTCTTCTTACCACGGAAGAAACATTCGTTTGGTGCTGGAAGTAGAATCATTATAACAACTAGAGATAAGCATTTGCTGAGTAAAGTTGATGAGACGTATGAGCCCAGGTTTTTAGGTGATGATGAAGCTCTTGAACTCTTCGGGCAGTCTGCCTTCAGAACACTCCAACCCACAGGAGATTACACTCGTCTCTCAAGGCATGCCATAGAATATGCTCGAGGTTTACCGTTAGCACTTAAAGTCTTGGGAGCTTTCCTTGATAACAAAAGTGTAAATGAGTGGGAAGATGTGCTAGAGAAAATAAAGAAAGCCCCGCATTTAGGAATTCAGGATGTGCTTAGGACAAGCTTTGATGGGCTAGATGATAAAGAGAAGGACATTTTTCTCGATATTGTATGTTTCTTTAAAGGAATGAAAAAAGACTACGCAACAAAAGTTCTGGCTAGTAGTGGATTCTTCCCCCATAGTGGGTTAAGAGTTCTGGTTGATAAAGCTCTTGTGACTCTCTCACATCATGGTACACTTGAGATGCATGATTTGCTAGTTGACACAGGCAGGGAAATTGTCCGCCAAGAATCTATTAAAGAACCTGGGAAACGCAGTAGGTTGTGGAATTATGAAGATGTTGAGCGTGTGTTGACTCAAAATACGGCTACGGAAGCAGTTGAAAGCATAATCTTGGACTTGTCAAACTCATCGAAAGAGGTATGCTTAAAAACTGAAGCTTTTGAGAGAATGACGAGGCTAAGACTGCTGAAGATCCATTATAATCACTCCCTACACTCTGATGATGAAGACCTTTCATATAAGGATGCATTCCATCCAAGTGATTATTGTAAACAACACCTGAGTGGGGACTTGTCAAAGTTTTTTTCTCATGAGTTGAGGTGTCTCCTCTGGCATGGATTTCCCCAAAAATCTTTGCCATCCAATTTTCAGCCAAAAAATCTTATTGACCTTAACATGCGTTATAGTCACATGGAGAAACTTTGGGACGGAACCAAGTCTCTGAAAAAGTTGAACTTCATCGATTTAAGTCACTCTCAATATCTTACCAAAACCCCCGACTTCACTGAGGCAACAAATATTGAGAAACTAATTCTTGAAGGTTGTACAAGGTTACTTGAGGTTCACCCATCCATTTTAACTCTTACAAACCTGGTTGTTTTTAATCTAAAAGGGTGCAAGGAACTGAAGAGTTTCCCAAGCATTGTTCCTATGAAATCTCTTGGAACTCTCAATCTTTCTGGCTGCTCGAGTTTTGACAAGTTTCCAGAGATTTCCGAAATTATTGAGAGCTTGTCGGTGCTTCATTTAGCTGAGACTGCAATTAAAGAACTGCCCTCTTCAATTAAAAATCTTACAGGGCTTGCTTATATGAACCTAAAAGGTTGTGGAGAACTTGAGAGTCTTCCAAGCAGCATTTGTCAACTCAAGTCCCTTAGCTATGTTGCTCTTTCTGGTTGTTCAAAGCTCAAGCTGTTTCCAGATCTTGAAGAAAATATGGAAGGAATGAGAGAGCTTCATTTGGATGGAACATCTATCGAAGAGCTTTCCCCATCAATTAACCGGCTTATGAGGCTTGCAATTTTGAATCTGAGAAACTGCAAAAGCCTTATCTGTCTTCCGGAAAATATCTGTAAGTTGGCATGTCTTACAGGTCTCACGCTCTCCGGGTGCTCCAAACTTTCAAACTTGCCTGATAACTTGGGTAATTTAGAATCTTTGTGGGAACTTCAAATAGAAGGAAGTGGTATAAAGCATTTCCCATTCTCCATTTTACGCTTGAATAAGCTTTTATCGTTATCATGTGATGGATGTAAAGAACTTAGGGCACCCTTTTCATCATGGTCACCAATTAGAGTTCGTGGAGAGTCAGCACTTAGATATCATGGTTACTCTAGTCTGGAAAGTCTTGATTTAAGTGACTGCAATCTGTTGGAAATATCGGATTGTATTGCTCATTTGTCTTCAGTGAAAAGATTAAGTCTTTGCAGAAATGACTTTGAGACCTTACCACGAACAATGATTCACCTGCATGGATTAATGTCTCTTGAGCTGGATTCTTGTAAGAGACTGAAATCAATACCAATGCTTTCATCAAGTATAAGTTACATAGATGCCCATGATTGCACAGCTTTGGAAGAAGTTTCATCACCGAAACCTCATTGGGGGAATCTCTATTACTTCATATTTTCTAATTGCTCCCACCTGGAACTAAATCCATTTATGCATATTATGCTACGTCATTTGTGTTGGGATATCCTACCTCATTCTTTTTATACGTGTCTCCCTGGAAGTGAAGTTCCAGATTGGTTCAGCTATCGCTGTAGGGGGAGTTCAGTAAATGTGCAGCTACCACTGAATTTGTACGATGATAAATTTTCGGGATTTGCCATATGTGCTGTTTGCAACTTGCAGGGTGCTCACTCTGTATCTGATCTATCAGCTTTATGTTTCTGTAACTTCAAAGGAAATCATGGGCAGCACAGTTTCAGTTTCTATTTGTTCAAAGCAGGTTTAAGAGCCGACAGATTCTCTTTCCGAAAAAGAGGAACTGACAGAGTCCTGGAGTCAGATCACATGTTCCTGGGATATGTTCCATGGTCTGATTATTGCTTGATTGAAGGAGAGCGGGCCAGTGAACGCTTTTACACTGAGGCCACATTTACAATTATATTAGAAGATGGAATCTATACAAGTGATTTTGGGACAGAATTACGACGCCGTGCCATCTCAAGTTGTGGAGTTCGCTTCTTTGATGCTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1248

Amino Acids

141.53

Weight (kDa)

6.46

Isoelectric Point (pI)

41.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 21 - 200 2.4e-52 TIR domain
TIR_2 PF13676 24 - 120 6.8e-15 TIR domain
NB-ARC PF00931 217 - 377 4.6e-17 NB-ARC domain
WHD_ROQ1 PF23282 446 - 517 9.9e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 640 - 659 1.1e-08 Leucine Rich Repeat
LRR_14 PF23598 723 - 834 1.6e-10 Leucine-rich repeat region
LRR_13 PF23286 732 - 817 7.4e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 842 - 934 2.3e-09 Leucine-rich repeat region
LRR_13 PF23286 888 - 1043 3e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_8 PF13855 959 - 1014 5.2e-06 Leucine rich repeat
C-JID PF20160 1088 - 1242 6.3e-17 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1025, 1740
AarI CACCTGC 1 cut(s) 3015
Acc36I ACCTGC 2 cut(s) 3015, 3479
AccB1I GGYRCC 2 cut(s) 331, 2806
AccB7I CCANNNNNTGG 2 cut(s) 298, 328
AccBSI CCGCTC 1 cut(s) 3607
AccII CGCG 2 cut(s) 389, 496
AccIII TCCGGA 1 cut(s) 2599
AciI CCGC 7 cut(s) 367, 389, 426, 442, 1286, 1540, 3607
AclWI GGATC 6 cut(s) 208, 347, 360, 410, 873, 1726
AcsI RAATTY 5 cut(s) 143, 970, 1296, 3337, 3353
AcuI CTGAAG 5 cut(s) 1111, 1713, 1748, 2186, 2928
AcyI GRCGYC 1 cut(s) 3697
AdeI CACNNNGTG 1 cut(s) 273
AfaI GTAC 4 cut(s) 455, 1492, 2086, 3344
AflIII ACRYGT 3 cut(s) 69, 3257, 3555
AjiI CACGTC 1 cut(s) 70
AjnI CCWGG 7 cut(s) 1076, 1561, 2130, 3183, 3265, 3540, 3561
AleI CACNNNNGTG 1 cut(s) 1821
AloI GAACNNNNNNTCC 2 cut(s) 3261, 3293
Alw21I GWGCWC 4 cut(s) 177, 288, 2654, 3400
AlwI GGATC 6 cut(s) 208, 347, 360, 410, 873, 1726
AlwNI CAGNNNCTG 1 cut(s) 205
Ama87I CYCGRG 3 cut(s) 223, 1188, 2227
Aor13HI TCCGGA 1 cut(s) 2599
AoxI GGCC 3 cut(s) 638, 3609, 3633
ApeKI GCWGC 7 cut(s) 20, 23, 199, 2223, 2391, 3325, 3457
ApoI RAATTY 5 cut(s) 143, 970, 1296, 3337, 3353
AseI ATTAAT 2 cut(s) 14, 3017
Asp700I GAANNNNTTC 3 cut(s) 604, 816, 2171
AspS9I GGNCC 1 cut(s) 3609
AsuC2I CCSGG 1 cut(s) 2647
AsuHPI GGTGA 5 cut(s) 1100, 2096, 2817, 2996, 3120
AsuII TTCGAA 1 cut(s) 875
AvaI CYCGRG 3 cut(s) 223, 1188, 2227
BaeGI GKGCMC 2 cut(s) 336, 2809
BamHI GGATCC 1 cut(s) 352
BanI GGYRCC 2 cut(s) 331, 2806
BanII GRGCYC 3 cut(s) 177, 288, 1077
BbsI GAAGAC 5 cut(s) 99, 1773, 2375, 2588, 2934
Bbv12I GWGCWC 4 cut(s) 177, 288, 2654, 3400
BbvI GCAGC 7 cut(s) 7, 10, 211, 2210, 2403, 3337, 3469
BceAI ACGGC 3 cut(s) 225, 1638, 3684
BcgI CGANNNNNNTGC 4 cut(s) 404, 438, 494, 528
BciT130I CCWGG 7 cut(s) 1078, 1563, 2132, 3185, 3267, 3542, 3563
BcnI CCSGG 1 cut(s) 2647
BfaI CTAG 9 cut(s) 711, 825, 896, 1032, 1265, 1331, 1416, 1514, 2874
BfmI CTRYAG 1 cut(s) 3301
BfuAI ACCTGC 2 cut(s) 3015, 3479
BglII AGATCT 1 cut(s) 2461
BisI GCNGC 8 cut(s) 21, 24, 200, 426, 2224, 2392, 3326, 3458
BlsI GCNGC 8 cut(s) 22, 25, 201, 427, 2225, 2393, 3327, 3459
Bme1390I CCNGG 8 cut(s) 1078, 1563, 2132, 2647, 3185, 3267, 3542, 3563
BmeT110I CYCGRG 3 cut(s) 223, 1188, 2227
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 1 cut(s) 3609
BmiI GGNNCC 4 cut(s) 333, 354, 1972, 2808
BmrFI CCNGG 8 cut(s) 1078, 1563, 2132, 2647, 3185, 3267, 3542, 3563
BmsI GCATC 7 cut(s) 154, 269, 502, 1491, 1774, 3082, 3724
BpiI GAAGAC 5 cut(s) 99, 1773, 2375, 2588, 2934
BplI GAGNNNNNCTC 2 cut(s) 442, 474
BpmI CTGGAG 2 cut(s) 469, 3563
Bpu10I CCTNAGC 2 cut(s) 1310, 2414
Bpu14I TTCGAA 1 cut(s) 875
BpuEI CTTGAG 8 cut(s) 1150, 1517, 2117, 2390, 2396, 2432, 3047, 3694
BpuMI CCSGG 1 cut(s) 2647
Bsa29I ATCGAT 3 cut(s) 10, 162, 2001
BsaAI YACGTR 1 cut(s) 3258
BsaHI GRCGYC 1 cut(s) 3697
BsaI GGTCTC 2 cut(s) 2639, 2975
BsaJI CCNNGG 7 cut(s) 641, 983, 1076, 1562, 3265, 3562, 3575
BsaWI WCCGGW 2 cut(s) 482, 2599
Bse118I RCCGGY 1 cut(s) 2541
Bse1I ACTGG 2 cut(s) 303, 3612
BseAI TCCGGA 1 cut(s) 2599
BseBI CCWGG 7 cut(s) 1078, 1563, 2132, 3185, 3267, 3542, 3563
BseCI ATCGAT 3 cut(s) 10, 162, 2001
BseDI CCNNGG 7 cut(s) 641, 983, 1076, 1562, 3265, 3562, 3575
BseMII CTCAG 6 cut(s) 1040, 1811, 2037, 2283, 2561, 3621
BseNI ACTGG 2 cut(s) 303, 3612
BseRI GAGGAG 1 cut(s) 1856
BseSI GKGCMC 2 cut(s) 336, 2809
BseXI GCAGC 7 cut(s) 7, 10, 211, 2210, 2403, 3337, 3469
BseYI CCCAGC 1 cut(s) 961
BsgI GTGCAG 2 cut(s) 866, 3344
Bsh1236I CGCG 2 cut(s) 389, 496
Bsh1285I CGRYCG 2 cut(s) 10, 237
BshFI GGCC 3 cut(s) 640, 3611, 3635
BshNI GGYRCC 2 cut(s) 331, 2806
BshVI ATCGAT 3 cut(s) 10, 162, 2001
BsiEI CGRYCG 2 cut(s) 10, 237
BsiHKAI GWGCWC 4 cut(s) 177, 288, 2654, 3400
BsiHKCI CYCGRG 3 cut(s) 223, 1188, 2227
BsiSI CCGG 4 cut(s) 483, 2542, 2600, 2646
BslFI GGGAC 5 cut(s) 35, 1841, 1979, 2395, 3697
BsmBI CGTCTC 2 cut(s) 1058, 1166
BsmFI GGGAC 5 cut(s) 35, 1841, 1979, 2395, 3697
BsmI GAATGC 1 cut(s) 1787
BsnI GGCC 3 cut(s) 640, 3611, 3635
Bso31I GGTCTC 2 cut(s) 2639, 2975
BsoBI CYCGRG 3 cut(s) 223, 1188, 2227
Bsp119I TTCGAA 1 cut(s) 875
Bsp1286I GDGCHC 7 cut(s) 177, 288, 336, 1077, 2654, 2809, 3400
Bsp13I TCCGGA 1 cut(s) 2599
Bsp1407I TGTACA 1 cut(s) 2084
Bsp19I CCATGG 1 cut(s) 3575
Bsp68I TCGCGA 1 cut(s) 496
BspACI CCGC 7 cut(s) 367, 389, 426, 442, 1286, 1540, 3607
BspANI GGCC 3 cut(s) 640, 3611, 3635
BspCNI CTCAG 6 cut(s) 1041, 1812, 2038, 2284, 2562, 3622
BspDI ATCGAT 3 cut(s) 10, 162, 2001
BspEI TCCGGA 1 cut(s) 2599
BspFNI CGCG 2 cut(s) 389, 496
BspHI TCATGA 1 cut(s) 1849
BspLI GGNNCC 4 cut(s) 333, 354, 1972, 2808
BspMI ACCTGC 2 cut(s) 3015, 3479
BspPI GGATC 6 cut(s) 208, 347, 360, 410, 873, 1726
BspQI GCTCTTC 2 cut(s) 165, 2514
BspT104I TTCGAA 1 cut(s) 875
BspT107I GGYRCC 2 cut(s) 331, 2806
BspTNI GGTCTC 2 cut(s) 2639, 2975
BsrBI CCGCTC 1 cut(s) 3607
BsrFI RCCGGY 1 cut(s) 2541
BsrGI TGTACA 1 cut(s) 2084
BsrI ACTGG 2 cut(s) 303, 3612
BssAI RCCGGY 1 cut(s) 2541
BssECI CCNNGG 7 cut(s) 641, 983, 1076, 1562, 3265, 3562, 3575
BssNI GRCGYC 1 cut(s) 3697
BssT1I CCWWGG 2 cut(s) 641, 3575
Bst2UI CCWGG 7 cut(s) 1078, 1563, 2132, 3185, 3267, 3542, 3563
Bst4CI ACNGT 2 cut(s) 1200, 3464
Bst6I CTCTTC 7 cut(s) 37, 165, 371, 1115, 2162, 2323, 2514
BstACI GRCGYC 1 cut(s) 3697
BstAPI GCANNNNNTGC 3 cut(s) 814, 1510, 3211
BstAUI TGTACA 1 cut(s) 2084
BstBAI YACGTR 1 cut(s) 3258
BstBI TTCGAA 1 cut(s) 875
BstC8I GCNNGC 4 cut(s) 1173, 2343, 2556, 3609
BstDSI CCRYGG 2 cut(s) 983, 3575
BstEII GGTNACC 1 cut(s) 2823
BstFNI CGCG 2 cut(s) 389, 496
BstMCI CGRYCG 2 cut(s) 10, 237
BstMWI GCNNNNNNNGC 9 cut(s) 114, 814, 1510, 1631, 1723, 2297, 2551, 3211, 3374
BstNI CCWGG 7 cut(s) 1078, 1563, 2132, 3185, 3267, 3542, 3563
BstNSI RCATGY 4 cut(s) 1175, 1939, 2625, 3559
BstPI GGTNACC 1 cut(s) 2823
BstSCI CCNGG 8 cut(s) 1076, 1561, 2130, 2645, 3183, 3265, 3540, 3561
BstSFI CTRYAG 1 cut(s) 3301
BstSLI GKGCMC 2 cut(s) 336, 2809
BstUI CGCG 2 cut(s) 389, 496
BstV1I GCAGC 7 cut(s) 7, 10, 211, 2210, 2403, 3337, 3469
BstV2I GAAGAC 5 cut(s) 99, 1773, 2375, 2588, 2934
BstX2I RGATCY 3 cut(s) 352, 1731, 2461
BstYI RGATCY 3 cut(s) 352, 1731, 2461
Bsu15I ATCGAT 3 cut(s) 10, 162, 2001
BsuRI GGCC 3 cut(s) 640, 3611, 3635
BsuTUI ATCGAT 3 cut(s) 10, 162, 2001
BtgI CCRYGG 2 cut(s) 983, 3575
BtgZI GCGATG 1 cut(s) 691
BtrI CACGTC 1 cut(s) 70
BtsI GCAGTG 1 cut(s) 804
BtsIMutI CAGTG 6 cut(s) 804, 2043, 2952, 3332, 3619, 3627
BtuMI TCGCGA 1 cut(s) 496
BveI ACCTGC 2 cut(s) 3015, 3479
Cac8I GCNNGC 4 cut(s) 1173, 2343, 2556, 3609
CaiI CAGNNNCTG 1 cut(s) 205
CciI TCATGA 1 cut(s) 1849
Cfr10I RCCGGY 1 cut(s) 2541
Cfr13I GGNCC 1 cut(s) 3609
ClaI ATCGAT 3 cut(s) 10, 162, 2001
CseI GACGC 1 cut(s) 3705
CsiI ACCWGGT 1 cut(s) 2130
Csp6I GTAC 4 cut(s) 454, 1491, 2085, 3343
CspCI CAANNNNNGTGG 2 cut(s) 3321, 3356
CviQI GTAC 4 cut(s) 454, 1491, 2085, 3343
DraI TTTAAA 1 cut(s) 1378
DraIII CACNNNGTG 1 cut(s) 273
Eam1104I CTCTTC 7 cut(s) 37, 165, 371, 1115, 2162, 2323, 2514
EarI CTCTTC 7 cut(s) 37, 165, 371, 1115, 2162, 2323, 2514
EciI GGCGGA 1 cut(s) 1529
Ecl136II GAGCTC 2 cut(s) 175, 286
Eco130I CCWWGG 2 cut(s) 641, 3575
Eco147I AGGCCT 1 cut(s) 640
Eco24I GRGCYC 3 cut(s) 177, 288, 1077
Eco31I GGTCTC 2 cut(s) 2639, 2975
Eco32I GATATC 2 cut(s) 2861, 3235
Eco53kI GAGCTC 2 cut(s) 175, 286
Eco57I CTGAAG 5 cut(s) 1111, 1713, 1748, 2186, 2928
Eco88I CYCGRG 3 cut(s) 223, 1188, 2227
Eco91I GGTNACC 1 cut(s) 2823
EcoICRI GAGCTC 2 cut(s) 175, 286
EcoO65I GGTNACC 1 cut(s) 2823
EcoRI GAATTC 1 cut(s) 1296
EcoRII CCWGG 7 cut(s) 1076, 1561, 2130, 3183, 3265, 3540, 3561
EcoRV GATATC 2 cut(s) 2861, 3235
EcoT14I CCWWGG 2 cut(s) 641, 3575
EcoT22I ATGCAT 3 cut(s) 1506, 1789, 3207
EcoT38I GRGCYC 3 cut(s) 177, 288, 1077
ErhI CCWWGG 2 cut(s) 641, 3575
Esp3I CGTCTC 2 cut(s) 1058, 1166
FalI AAGNNNNNCTT 2 cut(s) 1979, 2011
FaqI GGGAC 5 cut(s) 35, 1841, 1979, 2395, 3697
FauI CCCGC 2 cut(s) 1293, 3600
FauNDI CATATG 2 cut(s) 804, 3371
Fnu4HI GCNGC 8 cut(s) 21, 24, 200, 426, 2224, 2392, 3326, 3458
FriOI GRGCYC 3 cut(s) 177, 288, 1077
Fsp4HI GCNGC 8 cut(s) 21, 24, 200, 426, 2224, 2392, 3326, 3458
FspBI CTAG 9 cut(s) 711, 825, 896, 1032, 1265, 1331, 1416, 1514, 2874
GluI GCNGC 8 cut(s) 21, 24, 200, 426, 2224, 2392, 3326, 3458
GsaI CCCAGC 1 cut(s) 965
GsuI CTGGAG 2 cut(s) 469, 3563
HaeIII GGCC 3 cut(s) 640, 3611, 3635
HapII CCGG 4 cut(s) 483, 2542, 2600, 2646
HgaI GACGC 1 cut(s) 3705
Hin1I GRCGYC 1 cut(s) 3697
HincII GTYRAC 3 cut(s) 1519, 1611, 2402
HindII GTYRAC 3 cut(s) 1519, 1611, 2402
HindIII AAGCTT 5 cut(s) 393, 518, 1318, 1695, 2765
HpaII CCGG 4 cut(s) 483, 2542, 2600, 2646
HphI GGTGA 5 cut(s) 1100, 2096, 2817, 2996, 3120
Hpy166II GTNNAC 9 cut(s) 934, 1196, 1493, 1519, 1611, 1811, 2104, 2402, 3617
Hpy8I GTNNAC 9 cut(s) 934, 1196, 1493, 1519, 1611, 1811, 2104, 2402, 3617
Hpy99I CGWCG 2 cut(s) 71, 3699
HpyCH4III ACNGT 2 cut(s) 1200, 3464
HpyCH4IV ACGT 4 cut(s) 69, 1067, 3217, 3257
HpyF10VI GCNNNNNNNGC 9 cut(s) 114, 814, 1510, 1631, 1723, 2297, 2551, 3211, 3374
HpySE526I ACGT 4 cut(s) 69, 1067, 3217, 3257
Hsp92I GRCGYC 1 cut(s) 3697
Kpn2I TCCGGA 1 cut(s) 2599
LguI GCTCTTC 2 cut(s) 165, 2514
LmnI GCTCC 6 cut(s) 180, 283, 444, 1220, 2657, 3182
Lsp1109I GCAGC 7 cut(s) 7, 10, 211, 2210, 2403, 3337, 3469
LweI GCATC 7 cut(s) 154, 269, 502, 1491, 1774, 3082, 3724
MabI ACCWGGT 1 cut(s) 2130
MaeI CTAG 9 cut(s) 711, 825, 896, 1032, 1265, 1331, 1416, 1514, 2874
MaeII ACGT 4 cut(s) 69, 1067, 3217, 3257
MaeIII GTNAC 9 cut(s) 1471, 1946, 2009, 2091, 2823, 2867, 2897, 3083, 3434
MbiI CCGCTC 1 cut(s) 3607
MflI RGATCY 3 cut(s) 352, 1731, 2461
MhlI GDGCHC 7 cut(s) 177, 288, 336, 1077, 2654, 2809, 3400
MlyI GAGTC 7 cut(s) 215, 1468, 1606, 2389, 2855, 3546, 3554
MmeI TCCRAC 5 cut(s) 38, 577, 649, 1162, 2892
Mph1103I ATGCAT 3 cut(s) 1506, 1789, 3207
MroI TCCGGA 1 cut(s) 2599
MroXI GAANNNNTTC 3 cut(s) 604, 816, 2171
MslI CAYNNNNRTG 5 cut(s) 1758, 1797, 1821, 2502, 3009
MspI CCGG 4 cut(s) 483, 2542, 2600, 2646
MspR9I CCNGG 8 cut(s) 1078, 1563, 2132, 2647, 3185, 3267, 3542, 3563
Mva1269I GAATGC 1 cut(s) 1787
MvaI CCWGG 7 cut(s) 1078, 1563, 2132, 3185, 3267, 3542, 3563
MvnI CGCG 2 cut(s) 389, 496
MwoI GCNNNNNNNGC 9 cut(s) 114, 814, 1510, 1631, 1723, 2297, 2551, 3211, 3374
NciI CCSGG 1 cut(s) 2647
NcoI CCATGG 1 cut(s) 3575
NdeI CATATG 2 cut(s) 804, 3371
NlaIV GGNNCC 4 cut(s) 333, 354, 1972, 2808
NmeAIII GCCGAG 1 cut(s) 427
NmuCI GTSAC 5 cut(s) 1471, 1946, 2009, 2823, 2897
NruI TCGCGA 1 cut(s) 496
NsiI ATGCAT 3 cut(s) 1506, 1789, 3207
NspI RCATGY 4 cut(s) 1175, 1939, 2625, 3559
NspV TTCGAA 1 cut(s) 875
OliI CACNNNNGTG 1 cut(s) 1821
PaeI GCATGC 1 cut(s) 1175
PaeR7I CTCGAG 3 cut(s) 223, 1188, 2227
PagI TCATGA 1 cut(s) 1849
PaqCI CACCTGC 1 cut(s) 3015
PceI AGGCCT 1 cut(s) 640
PciI ACATGT 1 cut(s) 3555
PciSI GCTCTTC 2 cut(s) 165, 2514
PcsI WCGNNNNNNNCGW 1 cut(s) 230
PctI GAATGC 1 cut(s) 1787
PdmI GAANNNNTTC 3 cut(s) 604, 816, 2171
PflFI GACNNNGTC 1 cut(s) 3536
PflMI CCANNNNNTGG 2 cut(s) 298, 328
PfoI TCCNGGA 1 cut(s) 3540
PkrI GCNGC 8 cut(s) 22, 25, 201, 427, 2225, 2393, 3327, 3459
Ple19I CGATCG 2 cut(s) 10, 237
PleI GAGTC 7 cut(s) 215, 1468, 1606, 2388, 2854, 3545, 3553
PpsI GAGTC 7 cut(s) 215, 1468, 1606, 2388, 2854, 3545, 3553
Ppu21I YACGTR 1 cut(s) 3258
PscI ACATGT 1 cut(s) 3555
PshBI ATTAAT 2 cut(s) 14, 3017
PsiI TTATAA 2 cut(s) 1025, 1740
Psp124BI GAGCTC 2 cut(s) 177, 288
Psp6I CCWGG 7 cut(s) 1076, 1561, 2130, 3183, 3265, 3540, 3561
PspEI GGTNACC 1 cut(s) 2823
PspFI CCCAGC 1 cut(s) 961
PspGI CCWGG 7 cut(s) 1076, 1561, 2130, 3183, 3265, 3540, 3561
PspN4I GGNNCC 4 cut(s) 333, 354, 1972, 2808
PspPI GGNCC 1 cut(s) 3609
PspXI VCTCGAGB 3 cut(s) 223, 1188, 2227
PsrI GAACNNNNNNTAC 2 cut(s) 3295, 3327
PstNI CAGNNNCTG 1 cut(s) 205
PsuI RGATCY 3 cut(s) 352, 1731, 2461
PsyI GACNNNGTC 1 cut(s) 3536
PvuI CGATCG 2 cut(s) 10, 237
RruI TCGCGA 1 cut(s) 496
RsaI GTAC 4 cut(s) 455, 1492, 2086, 3344
RsaNI GTAC 4 cut(s) 454, 1491, 2085, 3343
RseI CAYNNNNRTG 5 cut(s) 1758, 1797, 1821, 2502, 3009
SacI GAGCTC 2 cut(s) 177, 288
SapI GCTCTTC 2 cut(s) 165, 2514
SatI GCNGC 8 cut(s) 21, 24, 200, 426, 2224, 2392, 3326, 3458
Sau96I GGNCC 1 cut(s) 3609
SchI GAGTC 7 cut(s) 215, 1468, 1606, 2389, 2855, 3546, 3554
ScrFI CCNGG 8 cut(s) 1078, 1563, 2132, 2647, 3185, 3267, 3542, 3563
SduI GDGCHC 7 cut(s) 177, 288, 336, 1077, 2654, 2809, 3400
SexAI ACCWGGT 1 cut(s) 2130
SfaNI GCATC 7 cut(s) 154, 269, 502, 1491, 1774, 3082, 3724
SfcI CTRYAG 1 cut(s) 3301
Sfr274I CTCGAG 3 cut(s) 223, 1188, 2227
SfuI TTCGAA 1 cut(s) 875
SlaI CTCGAG 3 cut(s) 223, 1188, 2227
SmiMI CAYNNNNRTG 5 cut(s) 1758, 1797, 1821, 2502, 3009
SphI GCATGC 1 cut(s) 1175
SseBI AGGCCT 1 cut(s) 640
SsiI CCGC 7 cut(s) 367, 389, 426, 442, 1286, 1540, 3607
SspI AATATT 1 cut(s) 2059
SspMI CTAG 9 cut(s) 711, 825, 896, 1032, 1265, 1331, 1416, 1514, 2874
SstI GAGCTC 2 cut(s) 177, 288
StuI AGGCCT 1 cut(s) 640
StyD4I CCNGG 8 cut(s) 1076, 1561, 2130, 2645, 3183, 3265, 3540, 3561
StyI CCWWGG 2 cut(s) 641, 3575
TaaI ACNGT 2 cut(s) 1200, 3464
TaiI ACGT 4 cut(s) 72, 1070, 3220, 3260
TatI WGTACW 1 cut(s) 2084
TauI GCSGC 1 cut(s) 428
TscAI CASTG 6 cut(s) 804, 2050, 2952, 3339, 3619, 3634
TseFI GTSAC 5 cut(s) 1471, 1946, 2009, 2823, 2897
TseI GCWGC 7 cut(s) 20, 23, 199, 2223, 2391, 3325, 3457
Tsp45I GTSAC 5 cut(s) 1471, 1946, 2009, 2823, 2897
TspGWI ACGGA 4 cut(s) 543, 1000, 1643, 1983
TspRI CASTG 6 cut(s) 804, 2050, 2952, 3339, 3619, 3634
Tth111I GACNNNGTC 1 cut(s) 3536
Van91I CCANNNNNTGG 2 cut(s) 298, 328
VspI ATTAAT 2 cut(s) 14, 3017
XapI RAATTY 5 cut(s) 143, 970, 1296, 3337, 3353
XbaI TCTAGA 1 cut(s) 824
XceI RCATGY 4 cut(s) 1175, 1939, 2625, 3559
XhoI CTCGAG 3 cut(s) 223, 1188, 2227
XmnI GAANNNNTTC 3 cut(s) 604, 816, 2171
XspI CTAG 9 cut(s) 711, 825, 896, 1032, 1265, 1331, 1416, 1514, 2874
Zsp2I ATGCAT 3 cut(s) 1506, 1789, 3207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.