Rmu_sc0004618.1_g000001

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004618.1
OG0000067
Physical Location & Seq
Reverse (-)
1 .. 4595
4595 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004618.1_g000001.1.cds

Sequence Viewer

Length: 3528 bp
atgaattcccgcataaaagaaatggatttgctattatgtcatgggatatatgatgttcgcgttgttggaatatggggaatggttggcattggcaaaacaaccattgctagagctgtttatgacaaaatcgctcgtcaatttgagcacagttgctttcttgaaaatgtcaaggaacgtttcatgaagaatgatgcggtacaaatgcaagaagaactgctatctaggatgttgaaggaaaaagtgcgaagtgtaggcactttgagtagaggctcaaagatgatgatggaaaggctgggtaacaggaaagttttgcttgttctggatgatgtggaaaacactgcccagattgaagccttacttgggaagcaacattcatttggtcttggaagtaaaattattataacaagcagagaacaagcactgagtagagttgatgagacatatgtgcccaagtttttaagtgacgatgaagctcttgaactcttgaggaattatgccttcagaagaaaccagcccacaggagagagtgatgaactctcaaggcatgctgtggaatatgctcaaggtctgcctctagcacttagggttatgggatcttctcttcataacaaaagtgtatgtgaatgggaagatgtgttggaaaaaactaagaaaatcccacatatgaaatttcacaatgatcttataacaagttttgaggtactgaatgattcagagaaggaaatttttctagatatatcatgtttcttcaaaggaatggagaaagactacgtaactgaaattctggacagttgtggcttctttcccgaaagggaattaagagttctacttaatagagctcttgtaagtgtcactcgtgacaataagcttgagatgcatagattgctacagagaatgggttgggaaattgtccgacagcagtctattaaggagcacgggaaacccagtaggctgtggagtaacaaagatattcagaggttgttaactcaaaatacggctacagaacgaattgaaagtataaagctagacttgtgcaactcaaaagatgtatacataaacactaaatcttttaataaaatgacgagactaagattggtcagaatcgattacagtttggactggcttctccctgcgattccaagtgcagaccttaaacaatatgtgagtggggacttgaagtttctttctgatgagttgaggcttctctgctggcacggatgcccttactcatcattgccatccaactttaggccgaggaatcttatcgaccttgacttgcgttttagtcacattcaacgactttgggaaggaacaaagctcatgaaaatgttgaaattcgtcaatttaagtcactctatataccttgaaagaatccctgacttcagtgaggcgataaatctggagaaactaattctcgatggttgctcaagtttggttgaggtgcatccatccatttcagctcttcaaaaccttgttgtctttactttaaatgggtgtgaaaaacttcaggactttccgagcagcattcgtatgaaatctctcaaaacctttcaactttctggctgctcaagatttgagaagtttccaaagatttcagaagcaatggaggatctatcagagctttgtctagatgggactgcaattaaagaattgccctcatcaattgaactgcttcagggccttgaacttttaagtatgagaaactgcagaagccttgtccgtcttccacacaaaatatgtaatttggcaaaccttcaacagctctatctggctgggtgctcgaaactccataacttgcctggtaacttcggtaatttggaatccttgacagacctggaggtacaagatagtggtataaaacaacttccattctctgtactacatttgagcaagcttaagggggatgcattatcatgtattggatgtaaacaaatgacgacacccttttcggcatggccatcatcagttgaaaaatgttctagttactctgttctggtacatcttgatttaagtgactgcaatctgttggaaatatcaaatggtattgctcatttgtgttccctggaaacattaaagatgtgcagaaacaatttggagagcttacctgcaacaatgagtcaacttggtcgcctaacacatctcaaattggaggcttgcaagaggctgaaatcaattccacagctttcatcaagtgtgaagtacatagatgctcatgattgcacagctttagagtcagtgtcaacgccaaatcccccgtacgaaaagaatctttggttcacattttctaactgcttcccgctggtacaaacaaatctatttatagatgttgtggaaactcattcttatcatcagggtgatctgcaacctctttccttttacatgtgtcttcctggaagagaaattccagcttggttcagccatcagtgtaggggatcctcaatcactgtgcagctaccacccaattgtttccatgataactttctgggattttctatctgcgttgcttgtaactttgaggaagcttgtaattatccttctgatctatctgccctatgttcttgcacagctaaaggaaattatggggagcacagttttcagttgctccatccgggcttccgaactgacagattgtttgagttggatcacatgtttttggtatatgttccatggtttgaatatcacctaattgaagaaggagacccggtcaatgaaggctgtttcactgagggtttatcgttggctttcccccttcgacaaatttctcaggatttcaagatcttgttggtcatggctattgctcgtctggtagctatttctttccttcttgtttttgcttttgttttcttgctcggagcgccgccttggtgtcgattccctgtgattactgattgggagggtagtgttgttgtttggatgattgatcggaggccgagctctaacggcgtggatctggcttggactgagttggatgggcgagtctgggttttggatcggggtcgtcagatttccgacggtgatagggattgtacaatcgttttggttggccctggtggttcgctgcagcgtcatggtgttgccggagtggggttggcccgaccagttcaggggatcgatcaaaacttggaggatcttcaggttgtcctgggcgcctccgatcgggtttatcccgatctaggatctggtttcttcaatgattgcgaaggcattggaatggctttgaagttggttcgaggtgatcaggaatgggcgcgaacttgggtgtcctttgatcctggagatggtacagtatcgtcggcggtgaaccacggtgacggcggccatggggatggcggttttcagggcgatggagctcctccttgggctctatgggctggtgatccttctggggatggggctgctcaatgtggtggcctttttggctatgctggtgatggtctgaaggtggtcagtaggggaggcggcgacggcgacggcgacatgaaacgggattttttgggattttattcttctagaatagggtttcatgag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1176

Amino Acids

132.12

Weight (kDa)

5.86

Isoelectric Point (pI)

39.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 401, 686
Acc36I ACCTGC 1 cut(s) 2113
AccB1I GGYRCC 1 cut(s) 3146
AccI GTMKAC 1 cut(s) 1050
AccII CGCG 2 cut(s) 60, 3250
AciI CCGC 8 cut(s) 10, 194, 2297, 2858, 3296, 3315, 3330, 3459
AclI AACGTT 1 cut(s) 175
AcoI YGGCCR 2 cut(s) 1955, 3316
AcsI RAATTY 7 cut(s) 4, 668, 723, 780, 1334, 2400, 2757
AcuI CTGAAG 6 cut(s) 484, 1366, 1490, 1658, 3116, 3458
AcyI GRCGYC 1 cut(s) 3147
AfiI CCNNNNNNNGG 7 cut(s) 360, 1248, 3084, 3104, 3156, 3173, 3278
AflII CTTAAG 1 cut(s) 1895
AflIII ACRYGT 2 cut(s) 2379, 2646
AjnI CCWGG 7 cut(s) 1798, 1833, 2061, 2389, 3046, 3141, 3271
AjuI GAANNNNNNNTTGG 2 cut(s) 342, 374
Alw21I GWGCWC 7 cut(s) 147, 841, 936, 1781, 2589, 2936, 3352
Alw26I GTCTC 3 cut(s) 431, 1078, 2691
AoxI GGCC 8 cut(s) 1250, 1678, 1955, 2927, 3043, 3090, 3316, 3409
ApeKI GCWGC 6 cut(s) 1521, 1563, 2449, 3058, 3061, 3395
ApoI RAATTY 7 cut(s) 4, 668, 723, 780, 1334, 2400, 2757
ArsI GACNNNNNNTTYG 4 cut(s) 1930, 1962, 2615, 2647
Asp700I GAANNNNTTC 3 cut(s) 726, 1503, 1671
AspLEI GCGC 3 cut(s) 2857, 3149, 3250
AspS9I GGNCC 3 cut(s) 1678, 3044, 3091
AsuC2I CCSGG 2 cut(s) 2610, 2702
AsuHPI GGTGA 8 cut(s) 2366, 2672, 3026, 3245, 3310, 3320, 3386, 3440
BaeGI GKGCMC 1 cut(s) 450
BalI TGGCCA 1 cut(s) 1957
BamHI GGATCC 1 cut(s) 2432
BanI GGYRCC 1 cut(s) 3146
BanII GRGCYC 4 cut(s) 841, 2936, 3352, 3364
BarI GAAGNNNNNNTAC 2 cut(s) 2518, 2550
BauI CACGAG 1 cut(s) 855
BbsI GAAGAC 2 cut(s) 1715, 2378
Bbv12I GWGCWC 7 cut(s) 147, 841, 936, 1781, 2589, 2936, 3352
BbvI GCAGC 6 cut(s) 1533, 1550, 2461, 3045, 3073, 3382
BceAI ACGGC 5 cut(s) 1011, 2956, 3328, 3481, 3487
BciT130I CCWGG 7 cut(s) 1800, 1835, 2063, 2391, 3048, 3143, 3273
BclI TGATCA 1 cut(s) 3235
BcnI CCSGG 2 cut(s) 2610, 2702
BcoDI GTCTC 3 cut(s) 431, 1078, 2691
BfaI CTAG 9 cut(s) 108, 222, 575, 731, 1025, 1628, 1980, 3173, 3510
BfmI CTRYAG 4 cut(s) 887, 999, 1705, 3059
BfoI RGCGCY 2 cut(s) 2858, 3150
BfrI CTTAAG 1 cut(s) 1895
BfuAI ACCTGC 1 cut(s) 2113
BglI GCCNNNNNGGC 1 cut(s) 3417
BglII AGATCT 1 cut(s) 2775
BisI GCNGC 9 cut(s) 1522, 1564, 2450, 2858, 3059, 3062, 3316, 3396, 3460
BlsI GCNGC 9 cut(s) 1523, 1565, 2451, 2859, 3060, 3063, 3317, 3397, 3461
Bme1390I CCNGG 9 cut(s) 1800, 1835, 2063, 2391, 2610, 2702, 3048, 3143, 3273
BmgT120I GGNCC 3 cut(s) 1678, 3044, 3091
BmiI GGNNCC 2 cut(s) 2434, 3148
BmrFI CCNGG 9 cut(s) 1800, 1835, 2063, 2391, 2610, 2702, 3048, 3143, 3273
BmrI ACTGGG 1 cut(s) 939
BmsI GCATC 6 cut(s) 181, 864, 1208, 1453, 1894, 2197
BmuI ACTGGG 1 cut(s) 939
BoxI GACNNNNGTC 1 cut(s) 919
BpiI GAAGAC 2 cut(s) 1715, 2378
BpmI CTGGAG 3 cut(s) 1421, 1856, 3294
BpuEI CTTGAG 6 cut(s) 505, 523, 546, 890, 1411, 1552
BpuMI CCSGG 2 cut(s) 2610, 2702
Bsa29I ATCGAT 2 cut(s) 1104, 3111
BsaAI YACGTR 1 cut(s) 772
BsaHI GRCGYC 1 cut(s) 3147
BsaI GGTCTC 1 cut(s) 2691
BsaJI CCNNGG 9 cut(s) 1253, 2061, 2666, 2861, 3046, 3142, 3304, 3319, 3356
BsaXI ACNNNNNCTCC 2 cut(s) 2688, 2718
Bsc4I CCNNNNNNNGG 7 cut(s) 360, 1248, 3084, 3104, 3156, 3173, 3278
Bse1I ACTGG 3 cut(s) 945, 1124, 3098
Bse3DI GCAATG 3 cut(s) 102, 1232, 1608
BseBI CCWGG 7 cut(s) 1800, 1835, 2063, 2391, 3048, 3143, 3273
BseCI ATCGAT 2 cut(s) 1104, 3111
BseDI CCNNGG 9 cut(s) 1253, 2061, 2666, 2861, 3046, 3142, 3304, 3319, 3356
BseLI CCNNNNNNNGG 7 cut(s) 360, 1248, 3084, 3104, 3156, 3173, 3278
BseMI GCAATG 3 cut(s) 102, 1232, 1608
BseMII CTCAG 4 cut(s) 413, 2715, 2777, 2952
BseNI ACTGG 3 cut(s) 945, 1124, 3098
BseRI GAGGAG 1 cut(s) 3342
BseSI GKGCMC 1 cut(s) 450
BseXI GCAGC 6 cut(s) 1533, 1550, 2461, 3045, 3073, 3382
BseYI CCCAGC 2 cut(s) 292, 1772
BsgI GTGCAG 3 cut(s) 1164, 2101, 2468
Bsh1236I CGCG 2 cut(s) 60, 3250
Bsh1285I CGRYCG 1 cut(s) 3157
BshFI GGCC 8 cut(s) 1252, 1680, 1957, 2929, 3045, 3092, 3318, 3411
BshNI GGYRCC 1 cut(s) 3146
BshVI ATCGAT 2 cut(s) 1104, 3111
BsiEI CGRYCG 1 cut(s) 3157
BsiHKAI GWGCWC 7 cut(s) 147, 841, 936, 1781, 2589, 2936, 3352
BsiSI CCGG 3 cut(s) 2609, 2702, 3078
BsiWI CGTACG 1 cut(s) 2256
BslFI GGGAC 2 cut(s) 1184, 1648
BslI CCNNNNNNNGG 7 cut(s) 360, 1248, 3084, 3104, 3156, 3173, 3278
BsmAI GTCTC 3 cut(s) 431, 1078, 2691
BsmFI GGGAC 2 cut(s) 1184, 1648
BsmI GAATGC 1 cut(s) 1524
BsnI GGCC 8 cut(s) 1252, 1680, 1957, 2929, 3045, 3092, 3318, 3411
Bso31I GGTCTC 1 cut(s) 2691
Bsp1286I GDGCHC 9 cut(s) 147, 450, 841, 936, 1781, 2589, 2936, 3352, 3364
Bsp1407I TGTACA 1 cut(s) 3026
Bsp19I CCATGG 2 cut(s) 2666, 3319
BspACI CCGC 8 cut(s) 10, 194, 2297, 2858, 3296, 3315, 3330, 3459
BspANI GGCC 8 cut(s) 1252, 1680, 1957, 2929, 3045, 3092, 3318, 3411
BspCNI CTCAG 4 cut(s) 414, 2716, 2776, 2953
BspDI ATCGAT 2 cut(s) 1104, 3111
BspFNI CGCG 2 cut(s) 60, 3250
BspHI TCATGA 4 cut(s) 180, 1320, 2212, 3523
BspLI GGNNCC 2 cut(s) 2434, 3148
BspMAI CTGCAG 2 cut(s) 1709, 3063
BspMI ACCTGC 1 cut(s) 2113
BspQI GCTCTTC 1 cut(s) 1467
BspT107I GGYRCC 1 cut(s) 3146
BspTI CTTAAG 1 cut(s) 1895
BspTNI GGTCTC 1 cut(s) 2691
BsrDI GCAATG 3 cut(s) 102, 1232, 1608
BsrGI TGTACA 1 cut(s) 3026
BsrI ACTGG 3 cut(s) 945, 1124, 3098
BssECI CCNNGG 9 cut(s) 1253, 2061, 2666, 2861, 3046, 3142, 3304, 3319, 3356
BssNAI GTATAC 1 cut(s) 1051
BssNI GRCGYC 1 cut(s) 3147
BssSI CACGAG 1 cut(s) 855
BssT1I CCWWGG 4 cut(s) 2666, 2861, 3319, 3356
Bst1107I GTATAC 1 cut(s) 1051
Bst2BI CACGAG 1 cut(s) 855
Bst2UI CCWGG 7 cut(s) 1800, 1835, 2063, 2391, 3048, 3143, 3273
Bst4CI ACNGT 8 cut(s) 149, 791, 1112, 2446, 2591, 3014, 3286, 3308
Bst6I CTCTTC 3 cut(s) 606, 1467, 2389
BstACI GRCGYC 1 cut(s) 3147
BstAFI CTTAAG 1 cut(s) 1895
BstAPI GCANNNNNTGC 1 cut(s) 883
BstAUI TGTACA 1 cut(s) 3026
BstBAI YACGTR 1 cut(s) 772
BstC8I GCNNGC 4 cut(s) 546, 1211, 1892, 2155
BstDEI CTNAG 7 cut(s) 422, 581, 648, 1088, 2724, 2763, 2961
BstDSI CCRYGG 3 cut(s) 2666, 3304, 3319
BstFNI CGCG 2 cut(s) 60, 3250
BstH2I RGCGCY 2 cut(s) 2858, 3150
BstHHI GCGC 3 cut(s) 2857, 3149, 3250
BstMAI GTCTC 3 cut(s) 431, 1078, 2691
BstMCI CGRYCG 1 cut(s) 3157
BstMWI GCNNNNNNNGC 7 cut(s) 874, 883, 2854, 2940, 3368, 3417, 3465
BstNI CCWGG 7 cut(s) 1800, 1835, 2063, 2391, 3048, 3143, 3273
BstNSI RCATGY 3 cut(s) 548, 2383, 2650
BstPAI GACNNNNGTC 1 cut(s) 919
BstSCI CCNGG 9 cut(s) 1798, 1833, 2061, 2389, 2608, 2700, 3046, 3141, 3271
BstSFI CTRYAG 4 cut(s) 887, 999, 1705, 3059
BstSLI GKGCMC 1 cut(s) 450
BstSNI TACGTA 1 cut(s) 772
BstUI CGCG 2 cut(s) 60, 3250
BstV1I GCAGC 6 cut(s) 1533, 1550, 2461, 3045, 3073, 3382
BstV2I GAAGAC 2 cut(s) 1715, 2378
BstX2I RGATCY 7 cut(s) 593, 1609, 2432, 2775, 2947, 3127, 3176
BstXI CCANNNNNNTGG 1 cut(s) 3326
BstYI RGATCY 7 cut(s) 593, 1609, 2432, 2775, 2947, 3127, 3176
BstZ17I GTATAC 1 cut(s) 1051
Bsu15I ATCGAT 2 cut(s) 1104, 3111
BsuRI GGCC 8 cut(s) 1252, 1680, 1957, 2929, 3045, 3092, 3318, 3411
BsuTUI ATCGAT 2 cut(s) 1104, 3111
BtgI CCRYGG 3 cut(s) 2666, 3304, 3319
BtgZI GCGATG 1 cut(s) 3357
BtsI GCAGTG 1 cut(s) 336
BtsIMutI CAGTG 7 cut(s) 336, 419, 1390, 2241, 2429, 2442, 2721
BveI ACCTGC 1 cut(s) 2113
Cac8I GCNNGC 4 cut(s) 546, 1211, 1892, 2155
CciI TCATGA 4 cut(s) 180, 1320, 2212, 3523
CfoI GCGC 3 cut(s) 2857, 3149, 3250
Cfr13I GGNCC 3 cut(s) 1678, 3044, 3091
ClaI ATCGAT 2 cut(s) 1104, 3111
CseI GACGC 1 cut(s) 3053
DdeI CTNAG 7 cut(s) 422, 581, 648, 1088, 2724, 2763, 2961
DinI GGCGCC 1 cut(s) 3148
DraI TTTAAA 1 cut(s) 1488
EaeI YGGCCR 2 cut(s) 1955, 3316
Eam1104I CTCTTC 3 cut(s) 606, 1467, 2389
EarI CTCTTC 3 cut(s) 606, 1467, 2389
Ecl136II GAGCTC 3 cut(s) 839, 2934, 3350
Eco105I TACGTA 1 cut(s) 772
Eco130I CCWWGG 4 cut(s) 2666, 2861, 3319, 3356
Eco24I GRGCYC 4 cut(s) 841, 2936, 3352, 3364
Eco31I GGTCTC 1 cut(s) 2691
Eco53kI GAGCTC 3 cut(s) 839, 2934, 3350
Eco57I CTGAAG 6 cut(s) 484, 1366, 1490, 1658, 3116, 3458
EcoICRI GAGCTC 3 cut(s) 839, 2934, 3350
EcoO109I RGGNCCY 1 cut(s) 1678
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 7 cut(s) 1798, 1833, 2061, 2389, 3046, 3141, 3271
EcoT14I CCWWGG 4 cut(s) 2666, 2861, 3319, 3356
EcoT22I ATGCAT 2 cut(s) 879, 1909
EcoT38I GRGCYC 4 cut(s) 841, 2936, 3352, 3364
EgeI GGCGCC 1 cut(s) 3148
EheI GGCGCC 1 cut(s) 3148
ErhI CCWWGG 4 cut(s) 2666, 2861, 3319, 3356
FalI AAGNNNNNCTT 6 cut(s) 297, 329, 342, 374, 1013, 1045
FaqI GGGAC 2 cut(s) 1184, 1648
FauI CCCGC 2 cut(s) 17, 2304
FauNDI CATATG 2 cut(s) 442, 663
FbaI TGATCA 1 cut(s) 3235
FblI GTMKAC 1 cut(s) 1050
Fnu4HI GCNGC 9 cut(s) 1522, 1564, 2450, 2858, 3059, 3062, 3316, 3396, 3460
FriOI GRGCYC 4 cut(s) 841, 2936, 3352, 3364
Fsp4HI GCNGC 9 cut(s) 1522, 1564, 2450, 2858, 3059, 3062, 3316, 3396, 3460
FspBI CTAG 9 cut(s) 108, 222, 575, 731, 1025, 1628, 1980, 3173, 3510
GlaI GCGC 3 cut(s) 2856, 3148, 3249
GluI GCNGC 9 cut(s) 1522, 1564, 2450, 2858, 3059, 3062, 3316, 3396, 3460
GsaI CCCAGC 2 cut(s) 296, 1776
GsuI CTGGAG 3 cut(s) 1421, 1856, 3294
HaeII RGCGCY 2 cut(s) 2858, 3150
HaeIII GGCC 8 cut(s) 1252, 1680, 1957, 2929, 3045, 3092, 3318, 3411
HapII CCGG 3 cut(s) 2609, 2702, 3078
HgaI GACGC 1 cut(s) 3053
HhaI GCGC 3 cut(s) 2857, 3149, 3250
Hin1I GRCGYC 1 cut(s) 3147
Hin6I GCGC 3 cut(s) 2855, 3147, 3248
HinP1I GCGC 3 cut(s) 2855, 3147, 3248
HincII GTYRAC 3 cut(s) 984, 2120, 2241
HindII GTYRAC 3 cut(s) 984, 2120, 2241
HindIII AAGCTT 3 cut(s) 866, 1892, 2520
HpaI GTTAAC 1 cut(s) 984
HpaII CCGG 3 cut(s) 2609, 2702, 3078
HphI GGTGA 8 cut(s) 2366, 2672, 3026, 3245, 3310, 3320, 3386, 3440
Hpy166II GTNNAC 7 cut(s) 984, 1051, 1928, 2120, 2241, 2277, 3301
Hpy8I GTNNAC 7 cut(s) 984, 1051, 1928, 2120, 2241, 2277, 3301
Hpy99I CGWCG 4 cut(s) 3014, 3295, 3467, 3473
HpyCH4III ACNGT 8 cut(s) 149, 791, 1112, 2446, 2591, 3014, 3286, 3308
HpyCH4IV ACGT 2 cut(s) 175, 771
HpyF10VI GCNNNNNNNGC 7 cut(s) 874, 883, 2854, 2940, 3368, 3417, 3465
HpyF3I CTNAG 7 cut(s) 422, 581, 648, 1088, 2724, 2763, 2961
HpySE526I ACGT 2 cut(s) 175, 771
Hsp92I GRCGYC 1 cut(s) 3147
HspAI GCGC 3 cut(s) 2855, 3147, 3248
KasI GGCGCC 1 cut(s) 3146
Ksp22I TGATCA 1 cut(s) 3235
KspAI GTTAAC 1 cut(s) 984
LguI GCTCTTC 1 cut(s) 1467
LmnI GCTCC 6 cut(s) 931, 2584, 2607, 2852, 3347, 3355
Lsp1109I GCAGC 6 cut(s) 1533, 1550, 2461, 3045, 3073, 3382
LweI GCATC 6 cut(s) 181, 864, 1208, 1453, 1894, 2197
MaeI CTAG 9 cut(s) 108, 222, 575, 731, 1025, 1628, 1980, 3173, 3510
MaeII ACGT 2 cut(s) 175, 771
MfeI CAATTG 2 cut(s) 1662, 2461
MflI RGATCY 7 cut(s) 593, 1609, 2432, 2775, 2947, 3127, 3176
MhlI GDGCHC 9 cut(s) 147, 450, 841, 936, 1781, 2589, 2936, 3352, 3364
MlsI TGGCCA 1 cut(s) 1957
MluNI TGGCCA 1 cut(s) 1957
Mly113I GGCGCC 1 cut(s) 3147
MlyI GAGTC 3 cut(s) 2125, 2240, 2985
MmeI TCCRAC 8 cut(s) 46, 618, 937, 1266, 2007, 2619, 2946, 3033
Mox20I TGGCCA 1 cut(s) 1957
Mph1103I ATGCAT 2 cut(s) 879, 1909
MroXI GAANNNNTTC 3 cut(s) 726, 1503, 1671
MscI TGGCCA 1 cut(s) 1957
MslI CAYNNNNRTG 6 cut(s) 1325, 1529, 1912, 2352, 3209, 3324
Msp20I TGGCCA 1 cut(s) 1957
MspA1I CMGCKG 1 cut(s) 2299
MspCI CTTAAG 1 cut(s) 1895
MspI CCGG 3 cut(s) 2609, 2702, 3078
MspR9I CCNGG 9 cut(s) 1800, 1835, 2063, 2391, 2610, 2702, 3048, 3143, 3273
MunI CAATTG 2 cut(s) 1662, 2461
Mva1269I GAATGC 1 cut(s) 1524
MvaI CCWGG 7 cut(s) 1800, 1835, 2063, 2391, 3048, 3143, 3273
MvnI CGCG 2 cut(s) 60, 3250
MwoI GCNNNNNNNGC 7 cut(s) 874, 883, 2854, 2940, 3368, 3417, 3465
NarI GGCGCC 1 cut(s) 3147
NciI CCSGG 2 cut(s) 2610, 2702
NcoI CCATGG 2 cut(s) 2666, 3319
NdeI CATATG 2 cut(s) 442, 663
NlaIV GGNNCC 2 cut(s) 2434, 3148
NmeAIII GCCGAG 2 cut(s) 1278, 2955
NmuCI GTSAC 7 cut(s) 461, 850, 857, 1286, 1349, 2012, 3308
NsiI ATGCAT 2 cut(s) 879, 1909
NspI RCATGY 3 cut(s) 548, 2383, 2650
PaeI GCATGC 1 cut(s) 548
PagI TCATGA 4 cut(s) 180, 1320, 2212, 3523
PciI ACATGT 2 cut(s) 2379, 2646
PciSI GCTCTTC 1 cut(s) 1467
PcsI WCGNNNNNNNCGW 1 cut(s) 3471
PctI GAATGC 1 cut(s) 1524
PdmI GAANNNNTTC 3 cut(s) 726, 1503, 1671
PfeI GAWTC 8 cut(s) 710, 1101, 1135, 1258, 1371, 1820, 2266, 2871
Pfl23II CGTACG 1 cut(s) 2256
PflFI GACNNNGTC 1 cut(s) 2702
PfoI TCCNGGA 2 cut(s) 2389, 3271
PkrI GCNGC 9 cut(s) 1523, 1565, 2451, 2859, 3060, 3063, 3317, 3397, 3461
Ple19I CGATCG 1 cut(s) 3157
PleI GAGTC 3 cut(s) 2124, 2239, 2984
PluTI GGCGCC 1 cut(s) 3150
PpsI GAGTC 3 cut(s) 2124, 2239, 2984
Ppu21I YACGTR 1 cut(s) 772
PscI ACATGT 2 cut(s) 2379, 2646
PshAI GACNNNNGTC 1 cut(s) 919
PsiI TTATAA 2 cut(s) 401, 686
Psp124BI GAGCTC 3 cut(s) 841, 2936, 3352
Psp1406I AACGTT 1 cut(s) 175
Psp6I CCWGG 7 cut(s) 1798, 1833, 2061, 2389, 3046, 3141, 3271
PspFI CCCAGC 2 cut(s) 292, 1772
PspGI CCWGG 7 cut(s) 1798, 1833, 2061, 2389, 3046, 3141, 3271
PspLI CGTACG 1 cut(s) 2256
PspN4I GGNNCC 2 cut(s) 2434, 3148
PspPI GGNCC 3 cut(s) 1678, 3044, 3091
PstI CTGCAG 2 cut(s) 1709, 3063
PsuI RGATCY 7 cut(s) 593, 1609, 2432, 2775, 2947, 3127, 3176
PsyI GACNNNGTC 1 cut(s) 2702
PvuI CGATCG 1 cut(s) 3157
RseI CAYNNNNRTG 6 cut(s) 1325, 1529, 1912, 2352, 3209, 3324
SacI GAGCTC 3 cut(s) 841, 2936, 3352
SapI GCTCTTC 1 cut(s) 1467
SatI GCNGC 9 cut(s) 1522, 1564, 2450, 2858, 3059, 3062, 3316, 3396, 3460
Sau96I GGNCC 3 cut(s) 1678, 3044, 3091
SchI GAGTC 3 cut(s) 2125, 2240, 2985
ScrFI CCNGG 9 cut(s) 1800, 1835, 2063, 2391, 2610, 2702, 3048, 3143, 3273
SduI GDGCHC 9 cut(s) 147, 450, 841, 936, 1781, 2589, 2936, 3352, 3364
SfaNI GCATC 6 cut(s) 181, 864, 1208, 1453, 1894, 2197
SfcI CTRYAG 4 cut(s) 887, 999, 1705, 3059
SfoI GGCGCC 1 cut(s) 3148
SmiMI CAYNNNNRTG 6 cut(s) 1325, 1529, 1912, 2352, 3209, 3324
SmlI CTYRAG 7 cut(s) 484, 538, 561, 869, 1426, 1567, 1895
SmoI CTYRAG 7 cut(s) 484, 538, 561, 869, 1426, 1567, 1895
SnaBI TACGTA 1 cut(s) 772
SphI GCATGC 1 cut(s) 548
SsiI CCGC 8 cut(s) 10, 194, 2297, 2858, 3296, 3315, 3330, 3459
SspDI GGCGCC 1 cut(s) 3146
SspMI CTAG 9 cut(s) 108, 222, 575, 731, 1025, 1628, 1980, 3173, 3510
SstI GAGCTC 3 cut(s) 841, 2936, 3352
StyD4I CCNGG 9 cut(s) 1798, 1833, 2061, 2389, 2608, 2700, 3046, 3141, 3271
StyI CCWWGG 4 cut(s) 2666, 2861, 3319, 3356
TaaI ACNGT 8 cut(s) 149, 791, 1112, 2446, 2591, 3014, 3286, 3308
TaiI ACGT 2 cut(s) 178, 774
TaqI TCGA 8 cut(s) 1104, 1266, 1416, 1781, 2752, 2869, 3111, 3229
TatI WGTACW 3 cut(s) 1876, 2199, 3026
TauI GCSGC 3 cut(s) 2860, 3318, 3462
TfiI GAWTC 8 cut(s) 710, 1101, 1135, 1258, 1371, 1820, 2266, 2871
TscAI CASTG 7 cut(s) 343, 426, 1390, 2241, 2429, 2449, 2728
TseFI GTSAC 7 cut(s) 461, 850, 857, 1286, 1349, 2012, 3308
TseI GCWGC 6 cut(s) 1521, 1563, 2449, 3058, 3061, 3395
Tsp45I GTSAC 7 cut(s) 461, 850, 857, 1286, 1349, 2012, 3308
TspGWI ACGGA 2 cut(s) 1230, 1709
TspRI CASTG 7 cut(s) 343, 426, 1390, 2241, 2429, 2449, 2728
Tth111I GACNNNGTC 1 cut(s) 2702
Vha464I CTTAAG 1 cut(s) 1895
XapI RAATTY 7 cut(s) 4, 668, 723, 780, 1334, 2400, 2757
XbaI TCTAGA 3 cut(s) 730, 1627, 3509
XceI RCATGY 3 cut(s) 548, 2383, 2650
XcmI CCANNNNNNNNNTGG 1 cut(s) 1796
XmiI GTMKAC 1 cut(s) 1050
XmnI GAANNNNTTC 3 cut(s) 726, 1503, 1671
XspI CTAG 9 cut(s) 108, 222, 575, 731, 1025, 1628, 1980, 3173, 3510
Zsp2I ATGCAT 2 cut(s) 879, 1909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.