RNA splicing
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 994 |
| Acc16I | TGCGCA | 1 cut(s) | 513 |
| Acc65I | GGTACC | 1 cut(s) | 590 |
| AccB1I | GGYRCC | 2 cut(s) | 590, 739 |
| AccBSI | CCGCTC | 2 cut(s) | 656, 1264 |
| AccI | GTMKAC | 1 cut(s) | 786 |
| AciI | CCGC | 2 cut(s) | 654, 1264 |
| AclWI | GGATC | 2 cut(s) | 50, 63 |
| AcsI | RAATTY | 5 cut(s) | 325, 517, 526, 1112, 1391 |
| AcyI | GRCGYC | 2 cut(s) | 349, 991 |
| AfaI | GTAC | 1 cut(s) | 592 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 1069, 1218 |
| AflII | CTTAAG | 1 cut(s) | 182 |
| AflIII | ACRYGT | 2 cut(s) | 932, 1352 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1181 |
| AjnI | CCWGG | 1 cut(s) | 58 |
| AluBI | AGCT | 5 cut(s) | 49, 472, 1150, 1204, 1271 |
| AluI | AGCT | 5 cut(s) | 49, 472, 1150, 1204, 1271 |
| Alw26I | GTCTC | 3 cut(s) | 2, 797, 1090 |
| AlwI | GGATC | 2 cut(s) | 50, 63 |
| AoxI | GGCC | 2 cut(s) | 664, 981 |
| ApoI | RAATTY | 5 cut(s) | 325, 517, 526, 1112, 1391 |
| AseI | ATTAAT | 1 cut(s) | 135 |
| Asp718I | GGTACC | 1 cut(s) | 590 |
| AspLEI | GCGC | 1 cut(s) | 514 |
| AspS9I | GGNCC | 1 cut(s) | 982 |
| AsuHPI | GGTGA | 3 cut(s) | 101, 188, 309 |
| AsuII | TTCGAA | 1 cut(s) | 1373 |
| AxyI | CCTNAGG | 1 cut(s) | 234 |
| BaeGI | GKGCMC | 1 cut(s) | 1158 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 582, 615 |
| BamHI | GGATCC | 1 cut(s) | 55 |
| BanI | GGYRCC | 2 cut(s) | 590, 739 |
| BbsI | GAAGAC | 1 cut(s) | 318 |
| BccI | CCATC | 7 cut(s) | 410, 581, 733, 888, 948, 953, 1292 |
| BciT130I | CCWGG | 1 cut(s) | 60 |
| BciVI | GTATCC | 3 cut(s) | 240, 468, 569 |
| BclI | TGATCA | 1 cut(s) | 1249 |
| BcoDI | GTCTC | 3 cut(s) | 2, 797, 1090 |
| BfaI | CTAG | 5 cut(s) | 122, 833, 975, 1091, 1151 |
| BfmI | CTRYAG | 3 cut(s) | 18, 285, 1195 |
| BfrI | CTTAAG | 1 cut(s) | 182 |
| BfuI | GTATCC | 3 cut(s) | 240, 468, 569 |
| BisI | GCNGC | 1 cut(s) | 654 |
| BlsI | GCNGC | 1 cut(s) | 655 |
| Bme1390I | CCNGG | 1 cut(s) | 60 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1181 |
| BmgT120I | GGNCC | 1 cut(s) | 982 |
| BmiI | GGNNCC | 3 cut(s) | 57, 592, 741 |
| BmrFI | CCNGG | 1 cut(s) | 60 |
| BmsI | GCATC | 1 cut(s) | 170 |
| BpiI | GAAGAC | 1 cut(s) | 318 |
| Bpu14I | TTCGAA | 1 cut(s) | 1373 |
| BpuEI | CTTGAG | 2 cut(s) | 549, 1280 |
| BsaHI | GRCGYC | 2 cut(s) | 349, 991 |
| BsaI | GGTCTC | 1 cut(s) | 1090 |
| BsaJI | CCNNGG | 1 cut(s) | 59 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 1069, 1218 |
| Bse1I | ACTGG | 1 cut(s) | 1177 |
| Bse21I | CCTNAGG | 1 cut(s) | 234 |
| BseBI | CCWGG | 1 cut(s) | 60 |
| BseDI | CCNNGG | 1 cut(s) | 59 |
| BseGI | GGATG | 3 cut(s) | 899, 940, 1303 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 1069, 1218 |
| BseMII | CTCAG | 2 cut(s) | 459, 1131 |
| BseNI | ACTGG | 1 cut(s) | 1177 |
| BseSI | GKGCMC | 1 cut(s) | 1158 |
| BshFI | GGCC | 2 cut(s) | 666, 983 |
| BshNI | GGYRCC | 2 cut(s) | 590, 739 |
| BslFI | GGGAC | 1 cut(s) | 641 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 1069, 1218 |
| BsmAI | GTCTC | 3 cut(s) | 2, 797, 1090 |
| BsmFI | GGGAC | 1 cut(s) | 641 |
| BsmI | GAATGC | 2 cut(s) | 610, 1141 |
| BsnI | GGCC | 2 cut(s) | 666, 983 |
| Bso31I | GGTCTC | 1 cut(s) | 1090 |
| Bsp119I | TTCGAA | 1 cut(s) | 1373 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1158 |
| Bsp143I | GATC | 7 cut(s) | 55, 118, 534, 682, 1056, 1249, 1330 |
| BspACI | CCGC | 2 cut(s) | 654, 1264 |
| BspANI | GGCC | 2 cut(s) | 666, 983 |
| BspCNI | CTCAG | 2 cut(s) | 460, 1132 |
| BspHI | TCATGA | 1 cut(s) | 1276 |
| BspLI | GGNNCC | 3 cut(s) | 57, 592, 741 |
| BspPI | GGATC | 2 cut(s) | 50, 63 |
| BspT104I | TTCGAA | 1 cut(s) | 1373 |
| BspT107I | GGYRCC | 2 cut(s) | 590, 739 |
| BspTI | CTTAAG | 1 cut(s) | 182 |
| BspTNI | GGTCTC | 1 cut(s) | 1090 |
| BsrBI | CCGCTC | 2 cut(s) | 656, 1264 |
| BsrI | ACTGG | 1 cut(s) | 1177 |
| BssECI | CCNNGG | 1 cut(s) | 59 |
| BssMI | GATC | 7 cut(s) | 55, 118, 534, 682, 1056, 1249, 1330 |
| BssNAI | GTATAC | 1 cut(s) | 787 |
| BssNI | GRCGYC | 2 cut(s) | 349, 991 |
| Bst1107I | GTATAC | 1 cut(s) | 787 |
| Bst2UI | CCWGG | 1 cut(s) | 60 |
| Bst4CI | ACNGT | 5 cut(s) | 13, 107, 649, 720, 1126 |
| BstACI | GRCGYC | 2 cut(s) | 349, 991 |
| BstAFI | CTTAAG | 1 cut(s) | 182 |
| BstBI | TTCGAA | 1 cut(s) | 1373 |
| BstC8I | GCNNGC | 1 cut(s) | 390 |
| BstDEI | CTNAG | 4 cut(s) | 172, 234, 468, 1140 |
| BstF5I | GGATG | 3 cut(s) | 899, 940, 1303 |
| BstHHI | GCGC | 1 cut(s) | 514 |
| BstKTI | GATC | 7 cut(s) | 58, 121, 537, 685, 1059, 1252, 1333 |
| BstMAI | GTCTC | 3 cut(s) | 2, 797, 1090 |
| BstMBI | GATC | 7 cut(s) | 55, 118, 534, 682, 1056, 1249, 1330 |
| BstNI | CCWGG | 1 cut(s) | 60 |
| BstNSI | RCATGY | 3 cut(s) | 392, 936, 1356 |
| BstSCI | CCNGG | 1 cut(s) | 58 |
| BstSFI | CTRYAG | 3 cut(s) | 18, 285, 1195 |
| BstSLI | GKGCMC | 1 cut(s) | 1158 |
| BstV2I | GAAGAC | 1 cut(s) | 318 |
| BstX2I | RGATCY | 1 cut(s) | 55 |
| BstYI | RGATCY | 1 cut(s) | 55 |
| BstZ17I | GTATAC | 1 cut(s) | 787 |
| Bsu36I | CCTNAGG | 1 cut(s) | 234 |
| BsuI | GTATCC | 3 cut(s) | 240, 468, 569 |
| BsuRI | GGCC | 2 cut(s) | 666, 983 |
| BtsCI | GGATG | 3 cut(s) | 899, 940, 1303 |
| BtsIMutI | CAGTG | 1 cut(s) | 112 |
| Cac8I | GCNNGC | 1 cut(s) | 390 |
| CciI | TCATGA | 1 cut(s) | 1276 |
| CfoI | GCGC | 1 cut(s) | 514 |
| Cfr13I | GGNCC | 1 cut(s) | 982 |
| CseI | GACGC | 1 cut(s) | 338 |
| Csp6I | GTAC | 1 cut(s) | 591 |
| CviAII | CATG | 7 cut(s) | 389, 451, 480, 933, 1277, 1353, 1400 |
| CviQI | GTAC | 1 cut(s) | 591 |
| DdeI | CTNAG | 4 cut(s) | 172, 234, 468, 1140 |
| DpnI | GATC | 7 cut(s) | 57, 120, 536, 684, 1058, 1251, 1332 |
| DpnII | GATC | 7 cut(s) | 55, 118, 534, 682, 1056, 1249, 1330 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1181 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1181 |
| Eco147I | AGGCCT | 1 cut(s) | 666 |
| Eco31I | GGTCTC | 1 cut(s) | 1090 |
| Eco32I | GATATC | 1 cut(s) | 496 |
| Eco81I | CCTNAGG | 1 cut(s) | 234 |
| EcoRII | CCWGG | 1 cut(s) | 58 |
| EcoRV | GATATC | 1 cut(s) | 496 |
| EcoT22I | ATGCAT | 1 cut(s) | 163 |
| FaeI | CATG | 7 cut(s) | 392, 454, 483, 936, 1280, 1356, 1403 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 790, 822 |
| FaqI | GGGAC | 1 cut(s) | 641 |
| FatI | CATG | 7 cut(s) | 388, 450, 479, 932, 1276, 1352, 1399 |
| FbaI | TGATCA | 1 cut(s) | 1249 |
| FblI | GTMKAC | 1 cut(s) | 786 |
| Fnu4HI | GCNGC | 1 cut(s) | 654 |
| FokI | GGATG | 3 cut(s) | 906, 927, 1310 |
| Fsp4HI | GCNGC | 1 cut(s) | 654 |
| FspBI | CTAG | 5 cut(s) | 122, 833, 975, 1091, 1151 |
| FspI | TGCGCA | 1 cut(s) | 513 |
| GlaI | GCGC | 1 cut(s) | 513 |
| GluI | GCNGC | 1 cut(s) | 654 |
| HaeIII | GGCC | 2 cut(s) | 666, 983 |
| HgaI | GACGC | 1 cut(s) | 338 |
| HhaI | GCGC | 1 cut(s) | 514 |
| Hin1I | GRCGYC | 2 cut(s) | 349, 991 |
| Hin1II | CATG | 7 cut(s) | 392, 454, 483, 936, 1280, 1356, 1403 |
| Hin6I | GCGC | 1 cut(s) | 512 |
| HinP1I | GCGC | 1 cut(s) | 512 |
| HincII | GTYRAC | 1 cut(s) | 502 |
| HindII | GTYRAC | 1 cut(s) | 502 |
| HinfI | GANTC | 3 cut(s) | 314, 503, 1182 |
| HphI | GGTGA | 3 cut(s) | 101, 188, 309 |
| Hpy166II | GTNNAC | 3 cut(s) | 179, 502, 787 |
| Hpy188I | TCNGA | 4 cut(s) | 469, 682, 893, 1072 |
| Hpy188III | TCNNGA | 3 cut(s) | 1022, 1277, 1334 |
| Hpy8I | GTNNAC | 3 cut(s) | 179, 502, 787 |
| Hpy99I | CGWCG | 2 cut(s) | 542, 653 |
| HpyAV | CCTTC | 1 cut(s) | 1369 |
| HpyCH4III | ACNGT | 5 cut(s) | 13, 107, 649, 720, 1126 |
| HpyCH4IV | ACGT | 2 cut(s) | 252, 991 |
| HpyCH4V | TGCA | 7 cut(s) | 161, 368, 388, 398, 608, 1188, 1317 |
| HpyF3I | CTNAG | 4 cut(s) | 172, 234, 468, 1140 |
| HpySE526I | ACGT | 2 cut(s) | 252, 991 |
| Hsp92I | GRCGYC | 2 cut(s) | 349, 991 |
| Hsp92II | CATG | 7 cut(s) | 392, 454, 483, 936, 1280, 1356, 1403 |
| HspAI | GCGC | 1 cut(s) | 512 |
| KpnI | GGTACC | 1 cut(s) | 594 |
| Ksp22I | TGATCA | 1 cut(s) | 1249 |
| Kzo9I | GATC | 7 cut(s) | 55, 118, 534, 682, 1056, 1249, 1330 |
| LmnI | GCTCC | 1 cut(s) | 962 |
| LweI | GCATC | 1 cut(s) | 170 |
| MaeI | CTAG | 5 cut(s) | 122, 833, 975, 1091, 1151 |
| MaeII | ACGT | 2 cut(s) | 252, 991 |
| MaeIII | GTNAC | 5 cut(s) | 693, 720, 1120, 1168, 1357 |
| MalI | GATC | 7 cut(s) | 57, 120, 536, 684, 1058, 1251, 1332 |
| MbiI | CCGCTC | 2 cut(s) | 656, 1264 |
| MboI | GATC | 7 cut(s) | 55, 118, 534, 682, 1056, 1249, 1330 |
| MboII | GAAGA | 4 cut(s) | 323, 883, 968, 1340 |
| MfeI | CAATTG | 1 cut(s) | 816 |
| MflI | RGATCY | 1 cut(s) | 55 |
| MhlI | GDGCHC | 1 cut(s) | 1158 |
| MlyI | GAGTC | 3 cut(s) | 308, 497, 1191 |
| MmeI | TCCRAC | 1 cut(s) | 52 |
| MnlI | CCTC | 7 cut(s) | 51, 241, 331, 447, 610, 835, 973 |
| Mph1103I | ATGCAT | 1 cut(s) | 163 |
| MseI | TTAA | 8 cut(s) | 135, 183, 204, 267, 435, 474, 776, 1411 |
| MslI | CAYNNNNRTG | 3 cut(s) | 305, 393, 1351 |
| MspCI | CTTAAG | 1 cut(s) | 182 |
| MspR9I | CCNGG | 1 cut(s) | 60 |
| MunI | CAATTG | 1 cut(s) | 816 |
| Mva1269I | GAATGC | 2 cut(s) | 610, 1141 |
| MvaI | CCWGG | 1 cut(s) | 60 |
| NdeII | GATC | 7 cut(s) | 55, 118, 534, 682, 1056, 1249, 1330 |
| NlaIII | CATG | 7 cut(s) | 392, 454, 483, 936, 1280, 1356, 1403 |
| NlaIV | GGNNCC | 3 cut(s) | 57, 592, 741 |
| NmuCI | GTSAC | 4 cut(s) | 693, 1120, 1168, 1357 |
| NsbI | TGCGCA | 1 cut(s) | 513 |
| NsiI | ATGCAT | 1 cut(s) | 163 |
| NspI | RCATGY | 3 cut(s) | 392, 936, 1356 |
| NspV | TTCGAA | 1 cut(s) | 1373 |
| PaeI | GCATGC | 1 cut(s) | 392 |
| PagI | TCATGA | 1 cut(s) | 1276 |
| PceI | AGGCCT | 1 cut(s) | 666 |
| PciI | ACATGT | 2 cut(s) | 932, 1352 |
| PctI | GAATGC | 2 cut(s) | 610, 1141 |
| PflFI | GACNNNGTC | 3 cut(s) | 107, 194, 1028 |
| PkrI | GCNGC | 1 cut(s) | 655 |
| PleI | GAGTC | 3 cut(s) | 308, 497, 1190 |
| PpsI | GAGTC | 3 cut(s) | 308, 497, 1190 |
| PscI | ACATGT | 2 cut(s) | 932, 1352 |
| PshBI | ATTAAT | 1 cut(s) | 135 |
| Psp6I | CCWGG | 1 cut(s) | 58 |
| PspGI | CCWGG | 1 cut(s) | 58 |
| PspN4I | GGNNCC | 3 cut(s) | 57, 592, 741 |
| PspPI | GGNCC | 1 cut(s) | 982 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 237, 269 |
| PsuI | RGATCY | 1 cut(s) | 55 |
| PsyI | GACNNNGTC | 3 cut(s) | 107, 194, 1028 |
| RsaI | GTAC | 1 cut(s) | 592 |
| RsaNI | GTAC | 1 cut(s) | 591 |
| RseI | CAYNNNNRTG | 3 cut(s) | 305, 393, 1351 |
| SaqAI | TTAA | 8 cut(s) | 135, 183, 204, 267, 435, 474, 776, 1411 |
| SatI | GCNGC | 1 cut(s) | 654 |
| Sau3AI | GATC | 7 cut(s) | 55, 118, 534, 682, 1056, 1249, 1330 |
| Sau96I | GGNCC | 1 cut(s) | 982 |
| SchI | GAGTC | 3 cut(s) | 308, 497, 1191 |
| ScrFI | CCNGG | 1 cut(s) | 60 |
| SduI | GDGCHC | 1 cut(s) | 1158 |
| SfaNI | GCATC | 1 cut(s) | 170 |
| SfcI | CTRYAG | 3 cut(s) | 18, 285, 1195 |
| SfuI | TTCGAA | 1 cut(s) | 1373 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 305, 393, 1351 |
| SmlI | CTYRAG | 3 cut(s) | 182, 564, 1259 |
| SmoI | CTYRAG | 3 cut(s) | 182, 564, 1259 |
| SphI | GCATGC | 1 cut(s) | 392 |
| SseBI | AGGCCT | 1 cut(s) | 666 |
| SsiI | CCGC | 2 cut(s) | 654, 1264 |
| SspI | AATATT | 1 cut(s) | 139 |
| SspMI | CTAG | 5 cut(s) | 122, 833, 975, 1091, 1151 |
| StuI | AGGCCT | 1 cut(s) | 666 |
| StyD4I | CCNGG | 1 cut(s) | 58 |
| TaaI | ACNGT | 5 cut(s) | 13, 107, 649, 720, 1126 |
| TaiI | ACGT | 2 cut(s) | 255, 994 |
| TaqI | TCGA | 3 cut(s) | 537, 602, 1373 |
| TauI | GCSGC | 1 cut(s) | 656 |
| Tru1I | TTAA | 8 cut(s) | 135, 183, 204, 267, 435, 474, 776, 1411 |
| Tru9I | TTAA | 8 cut(s) | 135, 183, 204, 267, 435, 474, 776, 1411 |
| TscAI | CASTG | 1 cut(s) | 112 |
| TseFI | GTSAC | 4 cut(s) | 693, 1120, 1168, 1357 |
| Tsp45I | GTSAC | 4 cut(s) | 693, 1120, 1168, 1357 |
| TspDTI | ATGAA | 2 cut(s) | 1025, 1234 |
| TspRI | CASTG | 1 cut(s) | 112 |
| Tth111I | GACNNNGTC | 3 cut(s) | 107, 194, 1028 |
| Vha464I | CTTAAG | 1 cut(s) | 182 |
| VspI | ATTAAT | 1 cut(s) | 135 |
| XapI | RAATTY | 5 cut(s) | 325, 517, 526, 1112, 1391 |
| XceI | RCATGY | 3 cut(s) | 392, 936, 1356 |
| XmiI | GTMKAC | 1 cut(s) | 786 |
| XspI | CTAG | 5 cut(s) | 122, 833, 975, 1091, 1151 |
| ZraI | GACGTC | 1 cut(s) | 992 |
| Zsp2I | ATGCAT | 1 cut(s) | 163 |