RNA splicing
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 2 cut(s) | 795, 877 |
| Acc36I | ACCTGC | 1 cut(s) | 142 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 777 |
| AccBSI | CCGCTC | 1 cut(s) | 999 |
| AccI | GTMKAC | 1 cut(s) | 282 |
| AccIII | TCCGGA | 1 cut(s) | 649 |
| AciI | CCGC | 4 cut(s) | 50, 206, 261, 999 |
| AclWI | GGATC | 2 cut(s) | 112, 1112 |
| AcoI | YGGCCR | 2 cut(s) | 768, 1057 |
| AcsI | RAATTY | 2 cut(s) | 225, 828 |
| AcyI | GRCGYC | 2 cut(s) | 792, 874 |
| AdeI | CACNNNGTG | 1 cut(s) | 1066 |
| AfaI | GTAC | 2 cut(s) | 507, 671 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 777, 952 |
| AgeI | ACCGGT | 1 cut(s) | 973 |
| AgsI | TTSAA | 7 cut(s) | 73, 524, 661, 754, 839, 960, 980 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 940, 972 |
| AleI | CACNNNNGTG | 1 cut(s) | 1010 |
| AluBI | AGCT | 3 cut(s) | 434, 494, 1033 |
| AluI | AGCT | 3 cut(s) | 434, 494, 1033 |
| Alw26I | GTCTC | 2 cut(s) | 419, 751 |
| AlwI | GGATC | 2 cut(s) | 112, 1112 |
| Aor13HI | TCCGGA | 1 cut(s) | 649 |
| AoxI | GGCC | 2 cut(s) | 768, 1057 |
| ApeKI | GCWGC | 1 cut(s) | 548 |
| ApoI | RAATTY | 2 cut(s) | 225, 828 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 264, 296 |
| AsiGI | ACCGGT | 1 cut(s) | 973 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 369 |
| AspS9I | GGNCC | 3 cut(s) | 267, 780, 1047 |
| AsuHPI | GGTGA | 5 cut(s) | 77, 478, 925, 963, 1024 |
| AsuII | TTCGAA | 1 cut(s) | 303 |
| AvaII | GGWCC | 3 cut(s) | 267, 780, 1047 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 653, 685 |
| BbsI | GAAGAC | 2 cut(s) | 250, 869 |
| BbvI | GCAGC | 1 cut(s) | 535 |
| BccI | CCATC | 3 cut(s) | 305, 598, 836 |
| BceAI | ACGGC | 2 cut(s) | 488, 783 |
| BciVI | GTATCC | 1 cut(s) | 363 |
| BclI | TGATCA | 1 cut(s) | 1128 |
| BcoDI | GTCTC | 2 cut(s) | 419, 751 |
| BfaI | CTAG | 5 cut(s) | 11, 62, 264, 573, 716 |
| BfmI | CTRYAG | 1 cut(s) | 174 |
| BfuAI | ACCTGC | 1 cut(s) | 142 |
| BfuI | GTATCC | 1 cut(s) | 363 |
| BglII | AGATCT | 2 cut(s) | 7, 634 |
| BisI | GCNGC | 1 cut(s) | 549 |
| BlsI | GCNGC | 1 cut(s) | 550 |
| Bme18I | GGWCC | 3 cut(s) | 267, 780, 1047 |
| BmgT120I | GGNCC | 3 cut(s) | 267, 780, 1047 |
| BmiI | GGNNCC | 1 cut(s) | 201 |
| BmsI | GCATC | 5 cut(s) | 199, 331, 391, 490, 882 |
| BpiI | GAAGAC | 2 cut(s) | 250, 869 |
| Bpu10I | CCTNAGC | 2 cut(s) | 490, 784 |
| Bpu14I | TTCGAA | 1 cut(s) | 303 |
| BpuEI | CTTGAG | 3 cut(s) | 420, 480, 560 |
| Bsa29I | ATCGAT | 1 cut(s) | 1074 |
| BsaHI | GRCGYC | 2 cut(s) | 792, 874 |
| BsaJI | CCNNGG | 3 cut(s) | 444, 552, 858 |
| BsaWI | WCCGGW | 2 cut(s) | 649, 973 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 777, 952 |
| Bse118I | RCCGGY | 4 cut(s) | 202, 694, 973, 1055 |
| Bse3DI | GCAATG | 3 cut(s) | 351, 689, 1114 |
| BseAI | TCCGGA | 1 cut(s) | 649 |
| BseCI | ATCGAT | 1 cut(s) | 1074 |
| BseDI | CCNNGG | 3 cut(s) | 444, 552, 858 |
| BseGI | GGATG | 5 cut(s) | 145, 214, 406, 880, 913 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 777, 952 |
| BseMI | GCAATG | 3 cut(s) | 351, 689, 1114 |
| BseMII | CTCAG | 1 cut(s) | 300 |
| BseRI | GAGGAG | 1 cut(s) | 746 |
| BseXI | GCAGC | 1 cut(s) | 535 |
| BsgI | GTGCAG | 2 cut(s) | 170, 551 |
| BshFI | GGCC | 2 cut(s) | 770, 1059 |
| BshTI | ACCGGT | 1 cut(s) | 973 |
| BshVI | ATCGAT | 1 cut(s) | 1074 |
| BsiSI | CCGG | 5 cut(s) | 203, 650, 695, 974, 1056 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 777, 952 |
| BsmAI | GTCTC | 2 cut(s) | 419, 751 |
| BsnI | GGCC | 2 cut(s) | 770, 1059 |
| Bsp119I | TTCGAA | 1 cut(s) | 303 |
| Bsp13I | TCCGGA | 1 cut(s) | 649 |
| Bsp143I | GATC | 6 cut(s) | 7, 117, 634, 741, 1104, 1128 |
| BspACI | CCGC | 4 cut(s) | 50, 206, 261, 999 |
| BspANI | GGCC | 2 cut(s) | 770, 1059 |
| BspCNI | CTCAG | 1 cut(s) | 299 |
| BspDI | ATCGAT | 1 cut(s) | 1074 |
| BspEI | TCCGGA | 1 cut(s) | 649 |
| BspHI | TCATGA | 1 cut(s) | 252 |
| BspLI | GGNNCC | 1 cut(s) | 201 |
| BspMAI | CTGCAG | 1 cut(s) | 178 |
| BspMI | ACCTGC | 1 cut(s) | 142 |
| BspPI | GGATC | 2 cut(s) | 112, 1112 |
| BspQI | GCTCTTC | 1 cut(s) | 989 |
| BspT104I | TTCGAA | 1 cut(s) | 303 |
| BsrBI | CCGCTC | 1 cut(s) | 999 |
| BsrDI | GCAATG | 3 cut(s) | 351, 689, 1114 |
| BsrFI | RCCGGY | 4 cut(s) | 202, 694, 973, 1055 |
| BssAI | RCCGGY | 4 cut(s) | 202, 694, 973, 1055 |
| BssECI | CCNNGG | 3 cut(s) | 444, 552, 858 |
| BssMI | GATC | 6 cut(s) | 7, 117, 634, 741, 1104, 1128 |
| BssNI | GRCGYC | 2 cut(s) | 792, 874 |
| BssT1I | CCWWGG | 2 cut(s) | 444, 858 |
| Bst4CI | ACNGT | 2 cut(s) | 529, 1009 |
| Bst6I | CTCTTC | 2 cut(s) | 749, 989 |
| BstACI | GRCGYC | 2 cut(s) | 792, 874 |
| BstBI | TTCGAA | 1 cut(s) | 303 |
| BstDEI | CTNAG | 3 cut(s) | 286, 490, 784 |
| BstF5I | GGATG | 5 cut(s) | 145, 214, 406, 880, 913 |
| BstKTI | GATC | 6 cut(s) | 10, 120, 637, 744, 1107, 1131 |
| BstMAI | GTCTC | 2 cut(s) | 419, 751 |
| BstMBI | GATC | 6 cut(s) | 7, 117, 634, 741, 1104, 1128 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 500, 1039 |
| BstNSI | RCATGY | 1 cut(s) | 1113 |
| BstSFI | CTRYAG | 1 cut(s) | 174 |
| BstV1I | GCAGC | 1 cut(s) | 535 |
| BstV2I | GAAGAC | 2 cut(s) | 250, 869 |
| BstX2I | RGATCY | 3 cut(s) | 7, 117, 634 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 238 |
| BstYI | RGATCY | 3 cut(s) | 7, 117, 634 |
| Bsu15I | ATCGAT | 1 cut(s) | 1074 |
| BsuI | GTATCC | 1 cut(s) | 363 |
| BsuRI | GGCC | 2 cut(s) | 770, 1059 |
| BsuTUI | ATCGAT | 1 cut(s) | 1074 |
| BtsCI | GGATG | 5 cut(s) | 145, 214, 406, 880, 913 |
| BveI | ACCTGC | 1 cut(s) | 142 |
| CciI | TCATGA | 1 cut(s) | 252 |
| Cfr10I | RCCGGY | 4 cut(s) | 202, 694, 973, 1055 |
| Cfr13I | GGNCC | 3 cut(s) | 267, 780, 1047 |
| ClaI | ATCGAT | 1 cut(s) | 1074 |
| Csp6I | GTAC | 2 cut(s) | 506, 670 |
| CspAI | ACCGGT | 1 cut(s) | 973 |
| CviAII | CATG | 4 cut(s) | 196, 253, 472, 1110 |
| CviQI | GTAC | 2 cut(s) | 506, 670 |
| DdeI | CTNAG | 3 cut(s) | 286, 490, 784 |
| DpnI | GATC | 6 cut(s) | 9, 119, 636, 743, 1106, 1130 |
| DpnII | GATC | 6 cut(s) | 7, 117, 634, 741, 1104, 1128 |
| DraIII | CACNNNGTG | 1 cut(s) | 1066 |
| EaeI | YGGCCR | 2 cut(s) | 768, 1057 |
| Eam1104I | CTCTTC | 2 cut(s) | 749, 989 |
| EarI | CTCTTC | 2 cut(s) | 749, 989 |
| EciI | GGCGGA | 1 cut(s) | 221 |
| Eco130I | CCWWGG | 2 cut(s) | 444, 858 |
| Eco47I | GGWCC | 3 cut(s) | 267, 780, 1047 |
| EcoT14I | CCWWGG | 2 cut(s) | 444, 858 |
| ErhI | CCWWGG | 2 cut(s) | 444, 858 |
| FaeI | CATG | 4 cut(s) | 199, 256, 475, 1113 |
| FatI | CATG | 4 cut(s) | 195, 252, 471, 1109 |
| FbaI | TGATCA | 1 cut(s) | 1128 |
| FblI | GTMKAC | 1 cut(s) | 282 |
| Fnu4HI | GCNGC | 1 cut(s) | 549 |
| FokI | GGATG | 5 cut(s) | 152, 221, 413, 867, 920 |
| Fsp4HI | GCNGC | 1 cut(s) | 549 |
| FspBI | CTAG | 5 cut(s) | 11, 62, 264, 573, 716 |
| GluI | GCNGC | 1 cut(s) | 549 |
| HaeIII | GGCC | 2 cut(s) | 770, 1059 |
| HapII | CCGG | 5 cut(s) | 203, 650, 695, 974, 1056 |
| Hin1I | GRCGYC | 2 cut(s) | 792, 874 |
| Hin1II | CATG | 4 cut(s) | 199, 256, 475, 1113 |
| HincII | GTYRAC | 1 cut(s) | 869 |
| HindII | GTYRAC | 1 cut(s) | 869 |
| HinfI | GANTC | 5 cut(s) | 377, 456, 936, 956, 1002 |
| HpaII | CCGG | 5 cut(s) | 203, 650, 695, 974, 1056 |
| HphI | GGTGA | 5 cut(s) | 77, 478, 925, 963, 1024 |
| Hpy166II | GTNNAC | 4 cut(s) | 283, 670, 780, 869 |
| Hpy188I | TCNGA | 2 cut(s) | 461, 955 |
| Hpy188III | TCNNGA | 6 cut(s) | 253, 362, 374, 437, 497, 650 |
| Hpy8I | GTNNAC | 4 cut(s) | 283, 670, 780, 869 |
| Hpy99I | CGWCG | 2 cut(s) | 794, 876 |
| HpyAV | CCTTC | 4 cut(s) | 45, 618, 715, 898 |
| HpyCH4III | ACNGT | 2 cut(s) | 529, 1009 |
| HpyCH4IV | ACGT | 2 cut(s) | 792, 874 |
| HpyCH4V | TGCA | 9 cut(s) | 151, 158, 176, 212, 344, 410, 532, 895, 1094 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 500, 1039 |
| HpyF3I | CTNAG | 3 cut(s) | 286, 490, 784 |
| HpySE526I | ACGT | 2 cut(s) | 792, 874 |
| Hsp92I | GRCGYC | 2 cut(s) | 792, 874 |
| Hsp92II | CATG | 4 cut(s) | 199, 256, 475, 1113 |
| Kpn2I | TCCGGA | 1 cut(s) | 649 |
| Ksp22I | TGATCA | 1 cut(s) | 1128 |
| Kzo9I | GATC | 6 cut(s) | 7, 117, 634, 741, 1104, 1128 |
| LguI | GCTCTTC | 1 cut(s) | 989 |
| LmnI | GCTCC | 2 cut(s) | 724, 845 |
| LpnPI | CCDG | 9 cut(s) | 137, 186, 216, 273, 375, 663, 708, 987, 1069 |
| Lsp1109I | GCAGC | 1 cut(s) | 535 |
| LweI | GCATC | 5 cut(s) | 199, 331, 391, 490, 882 |
| MaeI | CTAG | 5 cut(s) | 11, 62, 264, 573, 716 |
| MaeII | ACGT | 2 cut(s) | 792, 874 |
| MaeIII | GTNAC | 3 cut(s) | 326, 534, 913 |
| MalI | GATC | 6 cut(s) | 9, 119, 636, 743, 1106, 1130 |
| MbiI | CCGCTC | 1 cut(s) | 999 |
| MboI | GATC | 6 cut(s) | 7, 117, 634, 741, 1104, 1128 |
| MboII | GAAGA | 8 cut(s) | 255, 596, 638, 766, 851, 869, 1006, 1027 |
| MflI | RGATCY | 3 cut(s) | 7, 117, 634 |
| MluCI | AATT | 9 cut(s) | 68, 225, 273, 579, 629, 737, 801, 828, 948 |
| MlyI | GAGTC | 2 cut(s) | 465, 945 |
| MmeI | TCCRAC | 2 cut(s) | 135, 439 |
| MroI | TCCGGA | 1 cut(s) | 649 |
| MroXI | GAANNNNTTC | 1 cut(s) | 369 |
| MseI | TTAA | 1 cut(s) | 164 |
| MslI | CAYNNNNRTG | 2 cut(s) | 194, 1010 |
| MspI | CCGG | 5 cut(s) | 203, 650, 695, 974, 1056 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 500, 1039 |
| NdeII | GATC | 6 cut(s) | 7, 117, 634, 741, 1104, 1128 |
| NlaIII | CATG | 4 cut(s) | 199, 256, 475, 1113 |
| NlaIV | GGNNCC | 1 cut(s) | 201 |
| NmeAIII | GCCGAG | 1 cut(s) | 577 |
| NmuCI | GTSAC | 2 cut(s) | 534, 913 |
| NspI | RCATGY | 1 cut(s) | 1113 |
| NspV | TTCGAA | 1 cut(s) | 303 |
| OliI | CACNNNNGTG | 1 cut(s) | 1010 |
| PagI | TCATGA | 1 cut(s) | 252 |
| PciSI | GCTCTTC | 1 cut(s) | 989 |
| PdmI | GAANNNNTTC | 1 cut(s) | 369 |
| PfeI | GAWTC | 3 cut(s) | 377, 956, 1002 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 777 |
| PinAI | ACCGGT | 1 cut(s) | 973 |
| PkrI | GCNGC | 1 cut(s) | 550 |
| PleI | GAGTC | 2 cut(s) | 464, 944 |
| PpsI | GAGTC | 2 cut(s) | 464, 944 |
| PspN4I | GGNNCC | 1 cut(s) | 201 |
| PspPI | GGNCC | 3 cut(s) | 267, 780, 1047 |
| PstI | CTGCAG | 1 cut(s) | 178 |
| PsuI | RGATCY | 3 cut(s) | 7, 117, 634 |
| RsaI | GTAC | 2 cut(s) | 507, 671 |
| RsaNI | GTAC | 2 cut(s) | 506, 670 |
| RseI | CAYNNNNRTG | 2 cut(s) | 194, 1010 |
| SapI | GCTCTTC | 1 cut(s) | 989 |
| SaqAI | TTAA | 1 cut(s) | 164 |
| SatI | GCNGC | 1 cut(s) | 549 |
| Sau3AI | GATC | 6 cut(s) | 7, 117, 634, 741, 1104, 1128 |
| Sau96I | GGNCC | 3 cut(s) | 267, 780, 1047 |
| SchI | GAGTC | 2 cut(s) | 465, 945 |
| SfaNI | GCATC | 5 cut(s) | 199, 331, 391, 490, 882 |
| SfcI | CTRYAG | 1 cut(s) | 174 |
| SfuI | TTCGAA | 1 cut(s) | 303 |
| SinI | GGWCC | 3 cut(s) | 267, 780, 1047 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 194, 1010 |
| SmlI | CTYRAG | 4 cut(s) | 435, 495, 539, 1138 |
| SmoI | CTYRAG | 4 cut(s) | 435, 495, 539, 1138 |
| Sse9I | AATT | 9 cut(s) | 68, 225, 273, 579, 629, 737, 801, 828, 948 |
| SsiI | CCGC | 4 cut(s) | 50, 206, 261, 999 |
| SspMI | CTAG | 5 cut(s) | 11, 62, 264, 573, 716 |
| StyI | CCWWGG | 2 cut(s) | 444, 858 |
| TaaI | ACNGT | 2 cut(s) | 529, 1009 |
| TaiI | ACGT | 2 cut(s) | 795, 877 |
| TaqI | TCGA | 7 cut(s) | 20, 303, 373, 744, 826, 927, 1074 |
| TasI | AATT | 9 cut(s) | 68, 225, 273, 579, 629, 737, 801, 828, 948 |
| TatI | WGTACW | 1 cut(s) | 669 |
| TfiI | GAWTC | 3 cut(s) | 377, 956, 1002 |
| Tru1I | TTAA | 1 cut(s) | 164 |
| Tru9I | TTAA | 1 cut(s) | 164 |
| TseFI | GTSAC | 2 cut(s) | 534, 913 |
| TseI | GCWGC | 1 cut(s) | 548 |
| Tsp45I | GTSAC | 2 cut(s) | 534, 913 |
| TspDTI | ATGAA | 7 cut(s) | 241, 429, 699, 812, 869, 1038, 1113 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 777 |
| VpaK11BI | GGWCC | 3 cut(s) | 267, 780, 1047 |
| XapI | RAATTY | 2 cut(s) | 225, 828 |
| XceI | RCATGY | 1 cut(s) | 1113 |
| XmiI | GTMKAC | 1 cut(s) | 282 |
| XmnI | GAANNNNTTC | 1 cut(s) | 369 |
| XspI | CTAG | 5 cut(s) | 11, 62, 264, 573, 716 |
| ZraI | GACGTC | 2 cut(s) | 793, 875 |