protein kinase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 358 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 513 |
| AclWI | GGATC | 2 cut(s) | 396, 680 |
| AcoI | YGGCCR | 2 cut(s) | 504, 522 |
| AcsI | RAATTY | 2 cut(s) | 5, 218 |
| AcuI | CTGAAG | 1 cut(s) | 330 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 129, 513 |
| AflIII | ACRYGT | 1 cut(s) | 236 |
| AgsI | TTSAA | 4 cut(s) | 31, 143, 397, 490 |
| AjiI | CACGTC | 1 cut(s) | 456 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 307, 339 |
| AluBI | AGCT | 2 cut(s) | 373, 658 |
| AluI | AGCT | 2 cut(s) | 373, 658 |
| Alw21I | GWGCWC | 1 cut(s) | 498 |
| Alw26I | GTCTC | 1 cut(s) | 416 |
| AlwI | GGATC | 2 cut(s) | 396, 680 |
| AoxI | GGCC | 3 cut(s) | 249, 504, 522 |
| ApeKI | GCWGC | 1 cut(s) | 263 |
| ApoI | RAATTY | 2 cut(s) | 5, 218 |
| AspLEI | GCGC | 1 cut(s) | 106 |
| AspS9I | GGNCC | 2 cut(s) | 133, 516 |
| AvaII | GGWCC | 2 cut(s) | 133, 516 |
| BanI | GGYRCC | 1 cut(s) | 358 |
| Bbv12I | GWGCWC | 1 cut(s) | 498 |
| BbvI | GCAGC | 1 cut(s) | 250 |
| BccI | CCATC | 4 cut(s) | 5, 250, 507, 622 |
| BceAI | ACGGC | 3 cut(s) | 212, 220, 519 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 242, 276 |
| BclI | TGATCA | 1 cut(s) | 64 |
| BcoDI | GTCTC | 1 cut(s) | 416 |
| BfaI | CTAG | 3 cut(s) | 572, 594, 662 |
| BisI | GCNGC | 1 cut(s) | 264 |
| BlsI | GCNGC | 1 cut(s) | 265 |
| Bme18I | GGWCC | 2 cut(s) | 133, 516 |
| BmgBI | CACGTC | 1 cut(s) | 456 |
| BmgT120I | GGNCC | 2 cut(s) | 133, 516 |
| BmiI | GGNNCC | 3 cut(s) | 134, 135, 360 |
| BmsI | GCATC | 1 cut(s) | 568 |
| BpuEI | CTTGAG | 2 cut(s) | 290, 389 |
| Bsa29I | ATCGAT | 1 cut(s) | 675 |
| BsaBI | GATNNNNATC | 1 cut(s) | 30 |
| BsaJI | CCNNGG | 3 cut(s) | 123, 202, 519 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 129, 513 |
| Bse118I | RCCGGY | 1 cut(s) | 609 |
| Bse3DI | GCAATG | 1 cut(s) | 380 |
| Bse8I | GATNNNNATC | 1 cut(s) | 30 |
| BseCI | ATCGAT | 1 cut(s) | 675 |
| BseDI | CCNNGG | 3 cut(s) | 123, 202, 519 |
| BseGI | GGATG | 2 cut(s) | 446, 518 |
| BseJI | GATNNNNATC | 1 cut(s) | 30 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 129, 513 |
| BseMI | GCAATG | 1 cut(s) | 380 |
| BseRI | GAGGAG | 3 cut(s) | 479, 482, 590 |
| BseXI | GCAGC | 1 cut(s) | 250 |
| BshFI | GGCC | 3 cut(s) | 251, 506, 524 |
| BshNI | GGYRCC | 1 cut(s) | 358 |
| BshVI | ATCGAT | 1 cut(s) | 675 |
| BsiHKAI | GWGCWC | 1 cut(s) | 498 |
| BsiSI | CCGG | 2 cut(s) | 386, 610 |
| BslFI | GGGAC | 2 cut(s) | 146, 180 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 129, 513 |
| BsmAI | GTCTC | 1 cut(s) | 416 |
| BsmBI | CGTCTC | 1 cut(s) | 416 |
| BsmFI | GGGAC | 2 cut(s) | 146, 180 |
| BsnI | GGCC | 3 cut(s) | 251, 506, 524 |
| Bsp1286I | GDGCHC | 1 cut(s) | 498 |
| Bsp143I | GATC | 4 cut(s) | 64, 388, 672, 676 |
| BspANI | GGCC | 3 cut(s) | 251, 506, 524 |
| BspDI | ATCGAT | 1 cut(s) | 675 |
| BspLI | GGNNCC | 3 cut(s) | 134, 135, 360 |
| BspPI | GGATC | 2 cut(s) | 396, 680 |
| BspT107I | GGYRCC | 1 cut(s) | 358 |
| BsrDI | GCAATG | 1 cut(s) | 380 |
| BsrFI | RCCGGY | 1 cut(s) | 609 |
| BssAI | RCCGGY | 1 cut(s) | 609 |
| BssECI | CCNNGG | 3 cut(s) | 123, 202, 519 |
| BssMI | GATC | 4 cut(s) | 64, 388, 672, 676 |
| BssT1I | CCWWGG | 2 cut(s) | 123, 519 |
| BstC8I | GCNNGC | 2 cut(s) | 209, 611 |
| BstDSI | CCRYGG | 1 cut(s) | 202 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 127 |
| BstF5I | GGATG | 2 cut(s) | 446, 518 |
| BstHHI | GCGC | 1 cut(s) | 106 |
| BstKTI | GATC | 4 cut(s) | 67, 391, 675, 679 |
| BstMAI | GTCTC | 1 cut(s) | 416 |
| BstMBI | GATC | 4 cut(s) | 64, 388, 672, 676 |
| BstNSI | RCATGY | 4 cut(s) | 211, 215, 240, 559 |
| BstV1I | GCAGC | 1 cut(s) | 250 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 163 |
| Bsu15I | ATCGAT | 1 cut(s) | 675 |
| BsuRI | GGCC | 3 cut(s) | 251, 506, 524 |
| BsuTUI | ATCGAT | 1 cut(s) | 675 |
| BtgI | CCRYGG | 1 cut(s) | 202 |
| BtgZI | GCGATG | 1 cut(s) | 545 |
| BtrI | CACGTC | 1 cut(s) | 456 |
| BtsCI | GGATG | 2 cut(s) | 446, 518 |
| BtsIMutI | CAGTG | 1 cut(s) | 354 |
| Cac8I | GCNNGC | 2 cut(s) | 209, 611 |
| CfoI | GCGC | 1 cut(s) | 106 |
| Cfr10I | RCCGGY | 1 cut(s) | 609 |
| Cfr13I | GGNCC | 2 cut(s) | 133, 516 |
| ClaI | ATCGAT | 1 cut(s) | 675 |
| CviAII | CATG | 5 cut(s) | 208, 212, 224, 237, 556 |
| CviJI | RGCY | 9 cut(s) | 251, 298, 373, 506, 524, 584, 602, 613, 658 |
| CviKI_1 | RGCY | 9 cut(s) | 251, 298, 373, 506, 524, 584, 602, 613, 658 |
| DpnI | GATC | 4 cut(s) | 66, 390, 674, 678 |
| DpnII | GATC | 4 cut(s) | 64, 388, 672, 676 |
| EaeI | YGGCCR | 2 cut(s) | 504, 522 |
| Eco130I | CCWWGG | 2 cut(s) | 123, 519 |
| Eco47I | GGWCC | 2 cut(s) | 133, 516 |
| Eco57I | CTGAAG | 1 cut(s) | 330 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 127 |
| EcoO109I | RGGNCCY | 1 cut(s) | 133 |
| EcoRI | GAATTC | 1 cut(s) | 218 |
| EcoT14I | CCWWGG | 2 cut(s) | 123, 519 |
| EcoT22I | ATGCAT | 2 cut(s) | 213, 561 |
| ErhI | CCWWGG | 2 cut(s) | 123, 519 |
| Esp3I | CGTCTC | 1 cut(s) | 416 |
| FaeI | CATG | 5 cut(s) | 211, 215, 227, 240, 559 |
| FaqI | GGGAC | 2 cut(s) | 146, 180 |
| FatI | CATG | 5 cut(s) | 207, 211, 223, 236, 555 |
| FbaI | TGATCA | 1 cut(s) | 64 |
| Fnu4HI | GCNGC | 1 cut(s) | 264 |
| FokI | GGATG | 2 cut(s) | 433, 525 |
| Fsp4HI | GCNGC | 1 cut(s) | 264 |
| FspBI | CTAG | 3 cut(s) | 572, 594, 662 |
| GlaI | GCGC | 1 cut(s) | 105 |
| GluI | GCNGC | 1 cut(s) | 264 |
| HaeIII | GGCC | 3 cut(s) | 251, 506, 524 |
| HapII | CCGG | 2 cut(s) | 386, 610 |
| HhaI | GCGC | 1 cut(s) | 106 |
| Hin1II | CATG | 5 cut(s) | 211, 215, 227, 240, 559 |
| Hin6I | GCGC | 1 cut(s) | 104 |
| HinP1I | GCGC | 1 cut(s) | 104 |
| HindIII | AAGCTT | 1 cut(s) | 656 |
| HinfI | GANTC | 5 cut(s) | 95, 119, 272, 352, 478 |
| HpaII | CCGG | 2 cut(s) | 386, 610 |
| Hpy188I | TCNGA | 2 cut(s) | 40, 692 |
| Hpy188III | TCNNGA | 4 cut(s) | 116, 368, 572, 662 |
| HpyAV | CCTTC | 2 cut(s) | 173, 354 |
| HpyCH4IV | ACGT | 2 cut(s) | 425, 455 |
| HpyCH4V | TGCA | 2 cut(s) | 211, 559 |
| HpySE526I | ACGT | 2 cut(s) | 425, 455 |
| Hsp92II | CATG | 5 cut(s) | 211, 215, 227, 240, 559 |
| HspAI | GCGC | 1 cut(s) | 104 |
| KflI | GGGWCCC | 1 cut(s) | 133 |
| KroI | GCCGGC | 1 cut(s) | 609 |
| KroNI | GCCGGC | 1 cut(s) | 611 |
| Ksp22I | TGATCA | 1 cut(s) | 64 |
| Kzo9I | GATC | 4 cut(s) | 64, 388, 672, 676 |
| LpnPI | CCDG | 5 cut(s) | 92, 333, 341, 399, 623 |
| Lsp1109I | GCAGC | 1 cut(s) | 250 |
| LweI | GCATC | 1 cut(s) | 568 |
| MaeI | CTAG | 3 cut(s) | 572, 594, 662 |
| MaeII | ACGT | 2 cut(s) | 425, 455 |
| MaeIII | GTNAC | 1 cut(s) | 339 |
| MalI | GATC | 4 cut(s) | 66, 390, 674, 678 |
| MboI | GATC | 4 cut(s) | 64, 388, 672, 676 |
| MboII | GAAGA | 1 cut(s) | 254 |
| MhlI | GDGCHC | 1 cut(s) | 498 |
| MluCI | AATT | 4 cut(s) | 5, 218, 309, 315 |
| MlyI | GAGTC | 2 cut(s) | 113, 281 |
| Mph1103I | ATGCAT | 2 cut(s) | 213, 561 |
| MroNI | GCCGGC | 1 cut(s) | 609 |
| MseI | TTAA | 2 cut(s) | 171, 375 |
| MslI | CAYNNNNRTG | 2 cut(s) | 212, 458 |
| MspI | CCGG | 2 cut(s) | 386, 610 |
| NaeI | GCCGGC | 1 cut(s) | 611 |
| NdeII | GATC | 4 cut(s) | 64, 388, 672, 676 |
| NgoMIV | GCCGGC | 1 cut(s) | 609 |
| NlaIII | CATG | 5 cut(s) | 211, 215, 227, 240, 559 |
| NlaIV | GGNNCC | 3 cut(s) | 134, 135, 360 |
| NmeAIII | GCCGAG | 1 cut(s) | 277 |
| NsiI | ATGCAT | 2 cut(s) | 213, 561 |
| NspI | RCATGY | 4 cut(s) | 211, 215, 240, 559 |
| PaeI | GCATGC | 1 cut(s) | 211 |
| PciI | ACATGT | 1 cut(s) | 236 |
| PdiI | GCCGGC | 1 cut(s) | 611 |
| PfeI | GAWTC | 3 cut(s) | 95, 352, 478 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 513 |
| PkrI | GCNGC | 1 cut(s) | 265 |
| PleI | GAGTC | 2 cut(s) | 113, 280 |
| PpsI | GAGTC | 2 cut(s) | 113, 280 |
| PpuMI | RGGWCCY | 1 cut(s) | 133 |
| PscI | ACATGT | 1 cut(s) | 236 |
| Psp5II | RGGWCCY | 1 cut(s) | 133 |
| PspN4I | GGNNCC | 3 cut(s) | 134, 135, 360 |
| PspPI | GGNCC | 2 cut(s) | 133, 516 |
| PspPPI | RGGWCCY | 1 cut(s) | 133 |
| RseI | CAYNNNNRTG | 2 cut(s) | 212, 458 |
| SaqAI | TTAA | 2 cut(s) | 171, 375 |
| SatI | GCNGC | 1 cut(s) | 264 |
| Sau3AI | GATC | 4 cut(s) | 64, 388, 672, 676 |
| Sau96I | GGNCC | 2 cut(s) | 133, 516 |
| SchI | GAGTC | 2 cut(s) | 113, 281 |
| SduI | GDGCHC | 1 cut(s) | 498 |
| SetI | ASST | 6 cut(s) | 346, 375, 428, 458, 521, 660 |
| SfaNI | GCATC | 1 cut(s) | 568 |
| SinI | GGWCC | 2 cut(s) | 133, 516 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 212, 458 |
| SmlI | CTYRAG | 2 cut(s) | 269, 368 |
| SmoI | CTYRAG | 2 cut(s) | 269, 368 |
| SphI | GCATGC | 1 cut(s) | 211 |
| Sse9I | AATT | 4 cut(s) | 5, 218, 309, 315 |
| SspMI | CTAG | 3 cut(s) | 572, 594, 662 |
| StyI | CCWWGG | 2 cut(s) | 123, 519 |
| TaiI | ACGT | 2 cut(s) | 428, 458 |
| TaqI | TCGA | 1 cut(s) | 675 |
| TasI | AATT | 4 cut(s) | 5, 218, 309, 315 |
| TfiI | GAWTC | 3 cut(s) | 95, 352, 478 |
| Tru1I | TTAA | 2 cut(s) | 171, 375 |
| Tru9I | TTAA | 2 cut(s) | 171, 375 |
| TscAI | CASTG | 1 cut(s) | 354 |
| TseI | GCWGC | 1 cut(s) | 263 |
| TspDTI | ATGAA | 1 cut(s) | 87 |
| TspRI | CASTG | 1 cut(s) | 354 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 513 |
| VpaK11BI | GGWCC | 2 cut(s) | 133, 516 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 127 |
| XapI | RAATTY | 2 cut(s) | 5, 218 |
| XbaI | TCTAGA | 2 cut(s) | 571, 661 |
| XceI | RCATGY | 4 cut(s) | 211, 215, 240, 559 |
| XspI | CTAG | 3 cut(s) | 572, 594, 662 |
| Zsp2I | ATGCAT | 2 cut(s) | 213, 561 |