Reverse transcriptase (RNA-dependent DNA polymerase)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1314 |
| AatII | GACGTC | 1 cut(s) | 992 |
| Acc36I | ACCTGC | 2 cut(s) | 68, 451 |
| AccB1I | GGYRCC | 1 cut(s) | 839 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 181 |
| AccI | GTMKAC | 1 cut(s) | 906 |
| AciI | CCGC | 1 cut(s) | 446 |
| AclI | AACGTT | 2 cut(s) | 771, 1328 |
| AclWI | GGATC | 7 cut(s) | 158, 171, 430, 451, 565, 695, 744 |
| AcsI | RAATTY | 3 cut(s) | 185, 931, 1208 |
| AcuI | CTGAAG | 1 cut(s) | 266 |
| AcyI | GRCGYC | 2 cut(s) | 989, 1302 |
| AfaI | GTAC | 1 cut(s) | 1202 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 46, 47, 181, 335, 449 |
| AjnI | CCWGG | 3 cut(s) | 43, 552, 724 |
| AleI | CACNNNNGTG | 1 cut(s) | 524 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 176, 208 |
| AluBI | AGCT | 7 cut(s) | 83, 98, 224, 244, 413, 501, 1222 |
| AluI | AGCT | 7 cut(s) | 83, 98, 224, 244, 413, 501, 1222 |
| Alw21I | GWGCWC | 1 cut(s) | 784 |
| Alw26I | GTCTC | 6 cut(s) | 344, 383, 996, 1133, 1231, 1281 |
| Alw44I | GTGCAC | 1 cut(s) | 780 |
| AlwI | GGATC | 7 cut(s) | 158, 171, 430, 451, 565, 695, 744 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 416 |
| AoxI | GGCC | 6 cut(s) | 249, 474, 506, 1119, 1128, 1179 |
| ApaLI | GTGCAC | 1 cut(s) | 780 |
| ApeKI | GCWGC | 3 cut(s) | 77, 80, 410 |
| ApoI | RAATTY | 3 cut(s) | 185, 931, 1208 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 251 |
| AspA2I | CCTAGG | 1 cut(s) | 692 |
| AspS9I | GGNCC | 3 cut(s) | 474, 507, 961 |
| AsuHPI | GGTGA | 2 cut(s) | 731, 1273 |
| AvaII | GGWCC | 1 cut(s) | 961 |
| AvrII | CCTAGG | 1 cut(s) | 692 |
| BaeGI | GKGCMC | 1 cut(s) | 784 |
| BamHI | GGATCC | 1 cut(s) | 163 |
| BanI | GGYRCC | 1 cut(s) | 839 |
| BbsI | GAAGAC | 1 cut(s) | 125 |
| Bbv12I | GWGCWC | 1 cut(s) | 784 |
| BbvCI | CCTCAGC | 1 cut(s) | 84 |
| BbvI | GCAGC | 3 cut(s) | 64, 92, 422 |
| BccI | CCATC | 5 cut(s) | 368, 723, 851, 1072, 1139 |
| BciT130I | CCWGG | 3 cut(s) | 45, 554, 726 |
| BcoDI | GTCTC | 6 cut(s) | 344, 383, 996, 1133, 1231, 1281 |
| BfaI | CTAG | 5 cut(s) | 204, 303, 404, 504, 693 |
| BfmI | CTRYAG | 2 cut(s) | 78, 543 |
| BfuAI | ACCTGC | 2 cut(s) | 68, 451 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1127 |
| BisI | GCNGC | 4 cut(s) | 78, 81, 411, 447 |
| BlnI | CCTAGG | 1 cut(s) | 692 |
| BlsI | GCNGC | 4 cut(s) | 79, 82, 412, 448 |
| Bme1390I | CCNGG | 3 cut(s) | 45, 554, 726 |
| Bme18I | GGWCC | 1 cut(s) | 961 |
| BmgT120I | GGNCC | 3 cut(s) | 474, 507, 961 |
| BmiI | GGNNCC | 4 cut(s) | 165, 441, 841, 963 |
| BmrFI | CCNGG | 3 cut(s) | 45, 554, 726 |
| BmsI | GCATC | 1 cut(s) | 397 |
| BpiI | GAAGAC | 1 cut(s) | 125 |
| Bpu10I | CCTNAGC | 2 cut(s) | 84, 915 |
| BpuEI | CTTGAG | 3 cut(s) | 201, 771, 1196 |
| BsaAI | YACGTR | 1 cut(s) | 526 |
| BsaHI | GRCGYC | 2 cut(s) | 989, 1302 |
| BsaI | GGTCTC | 4 cut(s) | 344, 383, 1133, 1281 |
| BsaJI | CCNNGG | 6 cut(s) | 44, 145, 552, 553, 692, 975 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 46, 47, 181, 335, 449 |
| Bse3DI | GCAATG | 1 cut(s) | 1076 |
| BseBI | CCWGG | 3 cut(s) | 45, 554, 726 |
| BseDI | CCNNGG | 6 cut(s) | 44, 145, 552, 553, 692, 975 |
| BseGI | GGATG | 6 cut(s) | 29, 36, 734, 843, 1120, 1164 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 46, 47, 181, 335, 449 |
| BseMI | GCAATG | 1 cut(s) | 1076 |
| BseMII | CTCAG | 2 cut(s) | 75, 1320 |
| BseRI | GAGGAG | 4 cut(s) | 362, 965, 968, 971 |
| BseSI | GKGCMC | 1 cut(s) | 784 |
| BseXI | GCAGC | 3 cut(s) | 64, 92, 422 |
| BsgI | GTGCAG | 2 cut(s) | 184, 990 |
| BshFI | GGCC | 6 cut(s) | 251, 476, 508, 1121, 1130, 1181 |
| BshNI | GGYRCC | 1 cut(s) | 839 |
| BsiHKAI | GWGCWC | 1 cut(s) | 784 |
| BslFI | GGGAC | 1 cut(s) | 535 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 46, 47, 181, 335, 449 |
| BsmAI | GTCTC | 6 cut(s) | 344, 383, 996, 1133, 1231, 1281 |
| BsmBI | CGTCTC | 1 cut(s) | 996 |
| BsmFI | GGGAC | 1 cut(s) | 535 |
| BsnI | GGCC | 6 cut(s) | 251, 476, 508, 1121, 1130, 1181 |
| Bso31I | GGTCTC | 4 cut(s) | 344, 383, 1133, 1281 |
| Bsp1286I | GDGCHC | 1 cut(s) | 784 |
| Bsp143I | GATC | 8 cut(s) | 163, 422, 456, 557, 700, 736, 1290, 1338 |
| Bsp19I | CCATGG | 1 cut(s) | 145 |
| BspACI | CCGC | 1 cut(s) | 446 |
| BspANI | GGCC | 6 cut(s) | 251, 476, 508, 1121, 1130, 1181 |
| BspCNI | CTCAG | 2 cut(s) | 76, 1319 |
| BspHI | TCATGA | 1 cut(s) | 814 |
| BspLI | GGNNCC | 4 cut(s) | 165, 441, 841, 963 |
| BspMAI | CTGCAG | 1 cut(s) | 82 |
| BspMI | ACCTGC | 2 cut(s) | 68, 451 |
| BspPI | GGATC | 7 cut(s) | 158, 171, 430, 451, 565, 695, 744 |
| BspT107I | GGYRCC | 1 cut(s) | 839 |
| BspTNI | GGTCTC | 4 cut(s) | 344, 383, 1133, 1281 |
| BsrDI | GCAATG | 1 cut(s) | 1076 |
| BssECI | CCNNGG | 6 cut(s) | 44, 145, 552, 553, 692, 975 |
| BssMI | GATC | 8 cut(s) | 163, 422, 456, 557, 700, 736, 1290, 1338 |
| BssNI | GRCGYC | 2 cut(s) | 989, 1302 |
| BssT1I | CCWWGG | 3 cut(s) | 145, 692, 975 |
| Bst2UI | CCWGG | 3 cut(s) | 45, 554, 726 |
| Bst4CI | ACNGT | 1 cut(s) | 1247 |
| Bst6I | CTCTTC | 1 cut(s) | 317 |
| BstACI | GRCGYC | 2 cut(s) | 989, 1302 |
| BstBAI | YACGTR | 1 cut(s) | 526 |
| BstC8I | GCNNGC | 2 cut(s) | 53, 867 |
| BstDEI | CTNAG | 4 cut(s) | 84, 915, 1197, 1306 |
| BstDSI | CCRYGG | 1 cut(s) | 145 |
| BstF5I | GGATG | 6 cut(s) | 29, 36, 734, 843, 1120, 1164 |
| BstKTI | GATC | 8 cut(s) | 166, 425, 459, 560, 703, 739, 1293, 1341 |
| BstMAI | GTCTC | 6 cut(s) | 344, 383, 996, 1133, 1231, 1281 |
| BstMBI | GATC | 8 cut(s) | 163, 422, 456, 557, 700, 736, 1290, 1338 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 275, 1127 |
| BstNI | CCWGG | 3 cut(s) | 45, 554, 726 |
| BstNSI | RCATGY | 1 cut(s) | 608 |
| BstSCI | CCNGG | 3 cut(s) | 43, 552, 724 |
| BstSFI | CTRYAG | 2 cut(s) | 78, 543 |
| BstSLI | GKGCMC | 1 cut(s) | 784 |
| BstV1I | GCAGC | 3 cut(s) | 64, 92, 422 |
| BstV2I | GAAGAC | 1 cut(s) | 125 |
| BstX2I | RGATCY | 2 cut(s) | 163, 422 |
| BstYI | RGATCY | 2 cut(s) | 163, 422 |
| BsuRI | GGCC | 6 cut(s) | 251, 476, 508, 1121, 1130, 1181 |
| BtgI | CCRYGG | 1 cut(s) | 145 |
| BtsCI | GGATG | 6 cut(s) | 29, 36, 734, 843, 1120, 1164 |
| BtsI | GCAGTG | 1 cut(s) | 290 |
| BtsIMutI | CAGTG | 1 cut(s) | 290 |
| BveI | ACCTGC | 2 cut(s) | 68, 451 |
| Cac8I | GCNNGC | 2 cut(s) | 53, 867 |
| CaiI | CAGNNNCTG | 1 cut(s) | 416 |
| CciI | TCATGA | 1 cut(s) | 814 |
| Cfr13I | GGNCC | 3 cut(s) | 474, 507, 961 |
| CseI | GACGC | 1 cut(s) | 1310 |
| Csp6I | GTAC | 1 cut(s) | 1201 |
| CviQI | GTAC | 1 cut(s) | 1201 |
| DdeI | CTNAG | 4 cut(s) | 84, 915, 1197, 1306 |
| DpnI | GATC | 8 cut(s) | 165, 424, 458, 559, 702, 738, 1292, 1340 |
| DpnII | GATC | 8 cut(s) | 163, 422, 456, 557, 700, 736, 1290, 1338 |
| Eam1104I | CTCTTC | 1 cut(s) | 317 |
| EarI | CTCTTC | 1 cut(s) | 317 |
| Eco130I | CCWWGG | 3 cut(s) | 145, 692, 975 |
| Eco147I | AGGCCT | 1 cut(s) | 251 |
| Eco31I | GGTCTC | 4 cut(s) | 344, 383, 1133, 1281 |
| Eco47I | GGWCC | 1 cut(s) | 961 |
| Eco57I | CTGAAG | 1 cut(s) | 266 |
| EcoO109I | RGGNCCY | 2 cut(s) | 474, 961 |
| EcoRII | CCWGG | 3 cut(s) | 43, 552, 724 |
| EcoT14I | CCWWGG | 3 cut(s) | 145, 692, 975 |
| ErhI | CCWWGG | 3 cut(s) | 145, 692, 975 |
| Esp3I | CGTCTC | 1 cut(s) | 996 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1118, 1150 |
| FaqI | GGGAC | 1 cut(s) | 535 |
| FblI | GTMKAC | 1 cut(s) | 906 |
| Fnu4HI | GCNGC | 4 cut(s) | 78, 81, 411, 447 |
| FokI | GGATG | 6 cut(s) | 16, 23, 741, 830, 1127, 1151 |
| Fsp4HI | GCNGC | 4 cut(s) | 78, 81, 411, 447 |
| FspBI | CTAG | 5 cut(s) | 204, 303, 404, 504, 693 |
| GluI | GCNGC | 4 cut(s) | 78, 81, 411, 447 |
| HaeIII | GGCC | 6 cut(s) | 251, 476, 508, 1121, 1130, 1181 |
| HgaI | GACGC | 1 cut(s) | 1310 |
| Hin1I | GRCGYC | 2 cut(s) | 989, 1302 |
| HincII | GTYRAC | 3 cut(s) | 433, 658, 942 |
| HindII | GTYRAC | 3 cut(s) | 433, 658, 942 |
| HinfI | GANTC | 3 cut(s) | 309, 481, 1192 |
| HphI | GGTGA | 2 cut(s) | 731, 1273 |
| Hpy166II | GTNNAC | 6 cut(s) | 308, 433, 658, 782, 907, 942 |
| Hpy188I | TCNGA | 3 cut(s) | 613, 1309, 1323 |
| Hpy188III | TCNNGA | 4 cut(s) | 815, 877, 1147, 1213 |
| Hpy8I | GTNNAC | 6 cut(s) | 308, 433, 658, 782, 907, 942 |
| HpyCH4III | ACNGT | 1 cut(s) | 1247 |
| HpyCH4IV | ACGT | 7 cut(s) | 525, 688, 771, 938, 971, 989, 1328 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 275, 1127 |
| HpyF3I | CTNAG | 4 cut(s) | 84, 915, 1197, 1306 |
| HpySE526I | ACGT | 7 cut(s) | 525, 688, 771, 938, 971, 989, 1328 |
| Hsp92I | GRCGYC | 2 cut(s) | 989, 1302 |
| Kzo9I | GATC | 8 cut(s) | 163, 422, 456, 557, 700, 736, 1290, 1338 |
| LmnI | GCTCC | 2 cut(s) | 221, 1219 |
| Lsp1109I | GCAGC | 3 cut(s) | 64, 92, 422 |
| LweI | GCATC | 1 cut(s) | 397 |
| MaeI | CTAG | 5 cut(s) | 204, 303, 404, 504, 693 |
| MaeII | ACGT | 7 cut(s) | 525, 688, 771, 938, 971, 989, 1328 |
| MaeIII | GTNAC | 4 cut(s) | 828, 877, 985, 1298 |
| MalI | GATC | 8 cut(s) | 165, 424, 458, 559, 702, 738, 1292, 1340 |
| MboI | GATC | 8 cut(s) | 163, 422, 456, 557, 700, 736, 1290, 1338 |
| MboII | GAAGA | 4 cut(s) | 125, 304, 1059, 1062 |
| MflI | RGATCY | 2 cut(s) | 163, 422 |
| MhlI | GDGCHC | 1 cut(s) | 784 |
| MluCI | AATT | 7 cut(s) | 185, 491, 745, 931, 1045, 1208, 1270 |
| MlyI | GAGTC | 1 cut(s) | 303 |
| MmeI | TCCRAC | 3 cut(s) | 697, 709, 734 |
| MroXI | GAANNNNTTC | 1 cut(s) | 251 |
| MseI | TTAA | 3 cut(s) | 92, 179, 935 |
| MslI | CAYNNNNRTG | 2 cut(s) | 206, 524 |
| MspA1I | CMGCKG | 2 cut(s) | 83, 98 |
| MspR9I | CCNGG | 3 cut(s) | 45, 554, 726 |
| MvaI | CCWGG | 3 cut(s) | 45, 554, 726 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 275, 1127 |
| NcoI | CCATGG | 1 cut(s) | 145 |
| NdeII | GATC | 8 cut(s) | 163, 422, 456, 557, 700, 736, 1290, 1338 |
| NlaIV | GGNNCC | 4 cut(s) | 165, 441, 841, 963 |
| NmuCI | GTSAC | 3 cut(s) | 877, 985, 1298 |
| NspI | RCATGY | 1 cut(s) | 608 |
| OliI | CACNNNNGTG | 1 cut(s) | 524 |
| PagI | TCATGA | 1 cut(s) | 814 |
| PasI | CCCWGGG | 1 cut(s) | 553 |
| PceI | AGGCCT | 1 cut(s) | 251 |
| PdmI | GAANNNNTTC | 1 cut(s) | 251 |
| PfeI | GAWTC | 2 cut(s) | 481, 1192 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 181 |
| PfoI | TCCNGGA | 1 cut(s) | 724 |
| PkrI | GCNGC | 4 cut(s) | 79, 82, 412, 448 |
| PleI | GAGTC | 1 cut(s) | 303 |
| PpsI | GAGTC | 1 cut(s) | 303 |
| Ppu21I | YACGTR | 1 cut(s) | 526 |
| PpuMI | RGGWCCY | 1 cut(s) | 961 |
| PsiI | TTATAA | 1 cut(s) | 1314 |
| Psp1406I | AACGTT | 2 cut(s) | 771, 1328 |
| Psp5II | RGGWCCY | 1 cut(s) | 961 |
| Psp6I | CCWGG | 3 cut(s) | 43, 552, 724 |
| PspGI | CCWGG | 3 cut(s) | 43, 552, 724 |
| PspN4I | GGNNCC | 4 cut(s) | 165, 441, 841, 963 |
| PspPI | GGNCC | 3 cut(s) | 474, 507, 961 |
| PspPPI | RGGWCCY | 1 cut(s) | 961 |
| PstI | CTGCAG | 1 cut(s) | 82 |
| PstNI | CAGNNNCTG | 1 cut(s) | 416 |
| PsuI | RGATCY | 2 cut(s) | 163, 422 |
| PvuII | CAGCTG | 2 cut(s) | 83, 98 |
| RsaI | GTAC | 1 cut(s) | 1202 |
| RsaNI | GTAC | 1 cut(s) | 1201 |
| RseI | CAYNNNNRTG | 2 cut(s) | 206, 524 |
| SaqAI | TTAA | 3 cut(s) | 92, 179, 935 |
| SatI | GCNGC | 4 cut(s) | 78, 81, 411, 447 |
| Sau3AI | GATC | 8 cut(s) | 163, 422, 456, 557, 700, 736, 1290, 1338 |
| Sau96I | GGNCC | 3 cut(s) | 474, 507, 961 |
| SchI | GAGTC | 1 cut(s) | 303 |
| ScrFI | CCNGG | 3 cut(s) | 45, 554, 726 |
| SduI | GDGCHC | 1 cut(s) | 784 |
| SfaNI | GCATC | 1 cut(s) | 397 |
| SfcI | CTRYAG | 2 cut(s) | 78, 543 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 1127 |
| SinI | GGWCC | 1 cut(s) | 961 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 206, 524 |
| SmlI | CTYRAG | 3 cut(s) | 216, 786, 1175 |
| SmoI | CTYRAG | 3 cut(s) | 216, 786, 1175 |
| Sse9I | AATT | 7 cut(s) | 185, 491, 745, 931, 1045, 1208, 1270 |
| SseBI | AGGCCT | 1 cut(s) | 251 |
| SsiI | CCGC | 1 cut(s) | 446 |
| SspMI | CTAG | 5 cut(s) | 204, 303, 404, 504, 693 |
| StuI | AGGCCT | 1 cut(s) | 251 |
| StyD4I | CCNGG | 3 cut(s) | 43, 552, 724 |
| StyI | CCWWGG | 3 cut(s) | 145, 692, 975 |
| TaaI | ACNGT | 1 cut(s) | 1247 |
| TaiI | ACGT | 7 cut(s) | 528, 691, 774, 941, 974, 992, 1331 |
| TasI | AATT | 7 cut(s) | 185, 491, 745, 931, 1045, 1208, 1270 |
| TauI | GCSGC | 1 cut(s) | 449 |
| TfiI | GAWTC | 2 cut(s) | 481, 1192 |
| Tru1I | TTAA | 3 cut(s) | 92, 179, 935 |
| Tru9I | TTAA | 3 cut(s) | 92, 179, 935 |
| TscAI | CASTG | 1 cut(s) | 290 |
| TseFI | GTSAC | 3 cut(s) | 877, 985, 1298 |
| TseI | GCWGC | 3 cut(s) | 77, 80, 410 |
| Tsp45I | GTSAC | 3 cut(s) | 877, 985, 1298 |
| TspDTI | ATGAA | 7 cut(s) | 116, 184, 224, 304, 356, 790, 803 |
| TspGWI | ACGGA | 1 cut(s) | 22 |
| TspRI | CASTG | 1 cut(s) | 290 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 181 |
| VneI | GTGCAC | 1 cut(s) | 780 |
| VpaK11BI | GGWCC | 1 cut(s) | 961 |
| XapI | RAATTY | 3 cut(s) | 185, 931, 1208 |
| XceI | RCATGY | 1 cut(s) | 608 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 560 |
| XmaJI | CCTAGG | 1 cut(s) | 692 |
| XmiI | GTMKAC | 1 cut(s) | 906 |
| XmnI | GAANNNNTTC | 1 cut(s) | 251 |
| XspI | CTAG | 5 cut(s) | 204, 303, 404, 504, 693 |
| ZraI | GACGTC | 1 cut(s) | 990 |