protein kinase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1006 |
| Acc36I | ACCTGC | 1 cut(s) | 1006 |
| AccB1I | GGYRCC | 1 cut(s) | 370 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 525 |
| AccI | GTMKAC | 2 cut(s) | 577, 622 |
| AccII | CGCG | 2 cut(s) | 941, 972 |
| AciI | CCGC | 3 cut(s) | 67, 293, 970 |
| AcoI | YGGCCR | 2 cut(s) | 240, 534 |
| AcsI | RAATTY | 1 cut(s) | 107 |
| AcuI | CTGAAG | 2 cut(s) | 234, 342 |
| AfaI | GTAC | 1 cut(s) | 955 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 525, 849 |
| AflIII | ACRYGT | 1 cut(s) | 791 |
| AjiI | CACGTC | 1 cut(s) | 401 |
| AjnI | CCWGG | 1 cut(s) | 237 |
| AluBI | AGCT | 3 cut(s) | 596, 781, 835 |
| AluI | AGCT | 3 cut(s) | 596, 781, 835 |
| Alw21I | GWGCWC | 1 cut(s) | 510 |
| Alw26I | GTCTC | 2 cut(s) | 40, 420 |
| AoxI | GGCC | 2 cut(s) | 240, 534 |
| ApoI | RAATTY | 1 cut(s) | 107 |
| AspS9I | GGNCC | 2 cut(s) | 528, 856 |
| AsuHPI | GGTGA | 4 cut(s) | 275, 711, 772, 1100 |
| AsuII | TTCGAA | 1 cut(s) | 493 |
| AvaII | GGWCC | 2 cut(s) | 528, 856 |
| BanI | GGYRCC | 1 cut(s) | 370 |
| Bbv12I | GWGCWC | 1 cut(s) | 510 |
| BccI | CCATC | 6 cut(s) | 97, 107, 262, 519, 634, 923 |
| BceAI | ACGGC | 4 cut(s) | 227, 483, 693, 856 |
| BciT130I | CCWGG | 1 cut(s) | 239 |
| BciVI | GTATCC | 1 cut(s) | 806 |
| BclI | TGATCA | 1 cut(s) | 880 |
| BcoDI | GTCTC | 2 cut(s) | 40, 420 |
| BfaI | CTAG | 1 cut(s) | 584 |
| BfuAI | ACCTGC | 1 cut(s) | 1006 |
| BfuI | GTATCC | 1 cut(s) | 806 |
| BisI | GCNGC | 2 cut(s) | 293, 970 |
| BlsI | GCNGC | 2 cut(s) | 294, 971 |
| Bme1390I | CCNGG | 1 cut(s) | 239 |
| Bme18I | GGWCC | 2 cut(s) | 528, 856 |
| BmgBI | CACGTC | 1 cut(s) | 401 |
| BmgT120I | GGNCC | 2 cut(s) | 528, 856 |
| BmiI | GGNNCC | 1 cut(s) | 372 |
| BmrFI | CCNGG | 1 cut(s) | 239 |
| BmsI | GCATC | 1 cut(s) | 1004 |
| BpmI | CTGGAG | 1 cut(s) | 768 |
| Bpu14I | TTCGAA | 1 cut(s) | 493 |
| BpuEI | CTTGAG | 1 cut(s) | 591 |
| BsaAI | YACGTR | 1 cut(s) | 794 |
| BsaJI | CCNNGG | 1 cut(s) | 531 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 741, 771 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 525, 849 |
| Bse118I | RCCGGY | 2 cut(s) | 442, 902 |
| Bse1I | ACTGG | 1 cut(s) | 722 |
| Bse3DI | GCAATG | 4 cut(s) | 392, 437, 477, 997 |
| BseBI | CCWGG | 1 cut(s) | 239 |
| BseDI | CCNNGG | 1 cut(s) | 531 |
| BseGI | GGATG | 2 cut(s) | 530, 1075 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 525, 849 |
| BseMI | GCAATG | 4 cut(s) | 392, 437, 477, 997 |
| BseNI | ACTGG | 1 cut(s) | 722 |
| BseRI | GAGGAG | 2 cut(s) | 491, 602 |
| Bsh1236I | CGCG | 2 cut(s) | 941, 972 |
| BshFI | GGCC | 2 cut(s) | 242, 536 |
| BshNI | GGYRCC | 1 cut(s) | 370 |
| BsiHKAI | GWGCWC | 1 cut(s) | 510 |
| BsiSI | CCGG | 2 cut(s) | 443, 903 |
| BslFI | GGGAC | 2 cut(s) | 106, 930 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 525, 849 |
| BsmAI | GTCTC | 2 cut(s) | 40, 420 |
| BsmFI | GGGAC | 2 cut(s) | 106, 930 |
| BsmI | GAATGC | 1 cut(s) | 772 |
| BsnI | GGCC | 2 cut(s) | 242, 536 |
| Bsp119I | TTCGAA | 1 cut(s) | 493 |
| Bsp1286I | GDGCHC | 1 cut(s) | 510 |
| Bsp143I | GATC | 2 cut(s) | 121, 880 |
| BspACI | CCGC | 3 cut(s) | 67, 293, 970 |
| BspANI | GGCC | 2 cut(s) | 242, 536 |
| BspFNI | CGCG | 2 cut(s) | 941, 972 |
| BspHI | TCATGA | 1 cut(s) | 54 |
| BspLI | GGNNCC | 1 cut(s) | 372 |
| BspMI | ACCTGC | 1 cut(s) | 1006 |
| BspT104I | TTCGAA | 1 cut(s) | 493 |
| BspT107I | GGYRCC | 1 cut(s) | 370 |
| BsrDI | GCAATG | 4 cut(s) | 392, 437, 477, 997 |
| BsrFI | RCCGGY | 2 cut(s) | 442, 902 |
| BsrI | ACTGG | 1 cut(s) | 722 |
| BssAI | RCCGGY | 2 cut(s) | 442, 902 |
| BssECI | CCNNGG | 1 cut(s) | 531 |
| BssMI | GATC | 2 cut(s) | 121, 880 |
| BssNAI | GTATAC | 1 cut(s) | 578 |
| BssT1I | CCWWGG | 1 cut(s) | 531 |
| Bst1107I | GTATAC | 1 cut(s) | 578 |
| Bst2UI | CCWGG | 1 cut(s) | 239 |
| Bst4CI | ACNGT | 2 cut(s) | 51, 757 |
| BstBAI | YACGTR | 1 cut(s) | 794 |
| BstBI | TTCGAA | 1 cut(s) | 493 |
| BstC8I | GCNNGC | 2 cut(s) | 67, 974 |
| BstF5I | GGATG | 2 cut(s) | 530, 1075 |
| BstFNI | CGCG | 2 cut(s) | 941, 972 |
| BstKTI | GATC | 2 cut(s) | 124, 883 |
| BstMAI | GTCTC | 2 cut(s) | 40, 420 |
| BstMBI | GATC | 2 cut(s) | 121, 880 |
| BstNI | CCWGG | 1 cut(s) | 239 |
| BstSCI | CCNGG | 1 cut(s) | 237 |
| BstUI | CGCG | 2 cut(s) | 941, 972 |
| BstZ17I | GTATAC | 1 cut(s) | 578 |
| BsuI | GTATCC | 1 cut(s) | 806 |
| BsuRI | GGCC | 2 cut(s) | 242, 536 |
| BtgZI | GCGATG | 1 cut(s) | 273 |
| BtrI | CACGTC | 1 cut(s) | 401 |
| BtsCI | GGATG | 2 cut(s) | 530, 1075 |
| BtsIMutI | CAGTG | 1 cut(s) | 366 |
| BveI | ACCTGC | 1 cut(s) | 1006 |
| Cac8I | GCNNGC | 2 cut(s) | 67, 974 |
| CciI | TCATGA | 1 cut(s) | 54 |
| Cfr10I | RCCGGY | 2 cut(s) | 442, 902 |
| Cfr13I | GGNCC | 2 cut(s) | 528, 856 |
| CseI | GACGC | 1 cut(s) | 169 |
| Csp6I | GTAC | 1 cut(s) | 954 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 387, 422 |
| CviAII | CATG | 4 cut(s) | 55, 248, 1018, 1066 |
| CviQI | GTAC | 1 cut(s) | 954 |
| DpnI | GATC | 2 cut(s) | 123, 882 |
| DpnII | GATC | 2 cut(s) | 121, 880 |
| EaeI | YGGCCR | 2 cut(s) | 240, 534 |
| Eco130I | CCWWGG | 1 cut(s) | 531 |
| Eco47I | GGWCC | 2 cut(s) | 528, 856 |
| Eco57I | CTGAAG | 2 cut(s) | 234, 342 |
| EcoRII | CCWGG | 1 cut(s) | 237 |
| EcoT14I | CCWWGG | 1 cut(s) | 531 |
| EcoT22I | ATGCAT | 2 cut(s) | 573, 1019 |
| ErhI | CCWWGG | 1 cut(s) | 531 |
| FaeI | CATG | 4 cut(s) | 58, 251, 1021, 1069 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 156, 188, 180, 212, 845, 877 |
| FaqI | GGGAC | 2 cut(s) | 106, 930 |
| FatI | CATG | 4 cut(s) | 54, 247, 1017, 1065 |
| FauI | CCCGC | 1 cut(s) | 74 |
| FbaI | TGATCA | 1 cut(s) | 880 |
| FblI | GTMKAC | 2 cut(s) | 577, 622 |
| Fnu4HI | GCNGC | 2 cut(s) | 293, 970 |
| FokI | GGATG | 2 cut(s) | 537, 1082 |
| Fsp4HI | GCNGC | 2 cut(s) | 293, 970 |
| FspBI | CTAG | 1 cut(s) | 584 |
| GluI | GCNGC | 2 cut(s) | 293, 970 |
| GsuI | CTGGAG | 1 cut(s) | 768 |
| HaeIII | GGCC | 2 cut(s) | 242, 536 |
| HapII | CCGG | 2 cut(s) | 443, 903 |
| HgaI | GACGC | 1 cut(s) | 169 |
| Hin1II | CATG | 4 cut(s) | 58, 251, 1021, 1069 |
| HincII | GTYRAC | 1 cut(s) | 623 |
| HindII | GTYRAC | 1 cut(s) | 623 |
| HinfI | GANTC | 7 cut(s) | 8, 152, 176, 284, 364, 624, 1091 |
| HpaII | CCGG | 2 cut(s) | 443, 903 |
| HphI | GGTGA | 4 cut(s) | 275, 711, 772, 1100 |
| Hpy166II | GTNNAC | 3 cut(s) | 578, 623, 1109 |
| Hpy188I | TCNGA | 2 cut(s) | 703, 1036 |
| Hpy188III | TCNNGA | 7 cut(s) | 55, 173, 380, 584, 608, 747, 926 |
| Hpy8I | GTNNAC | 3 cut(s) | 578, 623, 1109 |
| Hpy99I | CGWCG | 1 cut(s) | 624 |
| HpyAV | CCTTC | 3 cut(s) | 209, 366, 847 |
| HpyCH4III | ACNGT | 2 cut(s) | 51, 757 |
| HpyCH4IV | ACGT | 3 cut(s) | 400, 619, 793 |
| HpyCH4V | TGCA | 8 cut(s) | 247, 278, 571, 787, 819, 990, 995, 1017 |
| HpySE526I | ACGT | 3 cut(s) | 400, 619, 793 |
| Hsp92II | CATG | 4 cut(s) | 58, 251, 1021, 1069 |
| Ksp22I | TGATCA | 1 cut(s) | 880 |
| Kzo9I | GATC | 2 cut(s) | 121, 880 |
| LpnPI | CCDG | 9 cut(s) | 224, 251, 353, 456, 732, 735, 789, 916, 1011 |
| LweI | GCATC | 1 cut(s) | 1004 |
| MaeI | CTAG | 1 cut(s) | 584 |
| MaeII | ACGT | 3 cut(s) | 400, 619, 793 |
| MaeIII | GTNAC | 3 cut(s) | 401, 751, 905 |
| MalI | GATC | 2 cut(s) | 123, 882 |
| MboI | GATC | 2 cut(s) | 121, 880 |
| MboII | GAAGA | 4 cut(s) | 17, 131, 664, 775 |
| MfeI | CAATTG | 1 cut(s) | 207 |
| MhlI | GDGCHC | 1 cut(s) | 510 |
| MluCI | AATT | 7 cut(s) | 107, 207, 321, 485, 549, 668, 876 |
| MlyI | GAGTC | 1 cut(s) | 618 |
| MmeI | TCCRAC | 1 cut(s) | 788 |
| MnlI | CCTC | 8 cut(s) | 322, 469, 472, 580, 583, 604, 642, 652 |
| Mph1103I | ATGCAT | 2 cut(s) | 573, 1019 |
| MseI | TTAA | 6 cut(s) | 98, 227, 387, 543, 875, 1124 |
| MslI | CAYNNNNRTG | 2 cut(s) | 252, 470 |
| MspI | CCGG | 2 cut(s) | 443, 903 |
| MspR9I | CCNGG | 1 cut(s) | 239 |
| MunI | CAATTG | 1 cut(s) | 207 |
| Mva1269I | GAATGC | 1 cut(s) | 772 |
| MvaI | CCWGG | 1 cut(s) | 239 |
| MvnI | CGCG | 2 cut(s) | 941, 972 |
| NdeII | GATC | 2 cut(s) | 121, 880 |
| NlaIII | CATG | 4 cut(s) | 58, 251, 1021, 1069 |
| NlaIV | GGNNCC | 1 cut(s) | 372 |
| NmeAIII | GCCGAG | 1 cut(s) | 796 |
| NmuCI | GTSAC | 3 cut(s) | 401, 751, 905 |
| NsiI | ATGCAT | 2 cut(s) | 573, 1019 |
| NspV | TTCGAA | 1 cut(s) | 493 |
| PagI | TCATGA | 1 cut(s) | 54 |
| PaqCI | CACCTGC | 1 cut(s) | 1006 |
| PctI | GAATGC | 1 cut(s) | 772 |
| PfeI | GAWTC | 6 cut(s) | 8, 152, 176, 284, 364, 1091 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 525 |
| PkrI | GCNGC | 2 cut(s) | 294, 971 |
| PleI | GAGTC | 1 cut(s) | 618 |
| PpsI | GAGTC | 1 cut(s) | 618 |
| Ppu21I | YACGTR | 1 cut(s) | 794 |
| Psp6I | CCWGG | 1 cut(s) | 237 |
| PspGI | CCWGG | 1 cut(s) | 237 |
| PspN4I | GGNNCC | 1 cut(s) | 372 |
| PspPI | GGNCC | 2 cut(s) | 528, 856 |
| RsaI | GTAC | 1 cut(s) | 955 |
| RsaNI | GTAC | 1 cut(s) | 954 |
| RseI | CAYNNNNRTG | 2 cut(s) | 252, 470 |
| SalI | GTCGAC | 1 cut(s) | 621 |
| SaqAI | TTAA | 6 cut(s) | 98, 227, 387, 543, 875, 1124 |
| SatI | GCNGC | 2 cut(s) | 293, 970 |
| Sau3AI | GATC | 2 cut(s) | 121, 880 |
| Sau96I | GGNCC | 2 cut(s) | 528, 856 |
| SchI | GAGTC | 1 cut(s) | 618 |
| ScrFI | CCNGG | 1 cut(s) | 239 |
| SduI | GDGCHC | 1 cut(s) | 510 |
| SfaNI | GCATC | 1 cut(s) | 1004 |
| SfuI | TTCGAA | 1 cut(s) | 493 |
| SinI | GGWCC | 2 cut(s) | 528, 856 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 252, 470 |
| SmlI | CTYRAG | 1 cut(s) | 606 |
| SmoI | CTYRAG | 1 cut(s) | 606 |
| Sse9I | AATT | 7 cut(s) | 107, 207, 321, 485, 549, 668, 876 |
| SsiI | CCGC | 3 cut(s) | 67, 293, 970 |
| SspMI | CTAG | 1 cut(s) | 584 |
| StyD4I | CCNGG | 1 cut(s) | 237 |
| StyI | CCWWGG | 1 cut(s) | 531 |
| TaaI | ACNGT | 2 cut(s) | 51, 757 |
| TaiI | ACGT | 3 cut(s) | 403, 622, 796 |
| TaqI | TCGA | 2 cut(s) | 493, 622 |
| TasI | AATT | 7 cut(s) | 107, 207, 321, 485, 549, 668, 876 |
| TauI | GCSGC | 2 cut(s) | 295, 972 |
| TfiI | GAWTC | 6 cut(s) | 8, 152, 176, 284, 364, 1091 |
| Tru1I | TTAA | 6 cut(s) | 98, 227, 387, 543, 875, 1124 |
| Tru9I | TTAA | 6 cut(s) | 98, 227, 387, 543, 875, 1124 |
| TscAI | CASTG | 1 cut(s) | 366 |
| TseFI | GTSAC | 3 cut(s) | 401, 751, 905 |
| Tsp45I | GTSAC | 3 cut(s) | 401, 751, 905 |
| TspDTI | ATGAA | 6 cut(s) | 43, 71, 144, 270, 1054, 1083 |
| TspRI | CASTG | 1 cut(s) | 366 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 525 |
| VpaK11BI | GGWCC | 2 cut(s) | 528, 856 |
| XapI | RAATTY | 1 cut(s) | 107 |
| XbaI | TCTAGA | 1 cut(s) | 583 |
| XmiI | GTMKAC | 2 cut(s) | 577, 622 |
| XspI | CTAG | 1 cut(s) | 584 |
| Zsp2I | ATGCAT | 2 cut(s) | 573, 1019 |