TMV resistance protein N-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 2191 |
| Acc36I | ACCTGC | 2 cut(s) | 807, 3485 |
| AccB1I | GGYRCC | 1 cut(s) | 270 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 124, 3120 |
| AccI | GTMKAC | 2 cut(s) | 1855, 1969 |
| AccII | CGCG | 2 cut(s) | 207, 209 |
| AccIII | TCCGGA | 1 cut(s) | 2351 |
| AciI | CCGC | 4 cut(s) | 1402, 2414, 2435, 3393 |
| AclI | AACGTT | 1 cut(s) | 1015 |
| AclWI | GGATC | 8 cut(s) | 341, 354, 936, 1254, 1469, 2014, 2280, 2619 |
| AcoI | YGGCCR | 2 cut(s) | 462, 2982 |
| AcuI | CTGAAG | 2 cut(s) | 1075, 3479 |
| AdeI | CACNNNGTG | 1 cut(s) | 270 |
| AfaI | GTAC | 8 cut(s) | 360, 624, 787, 1951, 2268, 2836, 3011, 3410 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 124, 1127, 2101, 3120, 3506 |
| AflII | CTTAAG | 1 cut(s) | 1774 |
| AflIII | ACRYGT | 2 cut(s) | 1429, 2815 |
| AhlI | ACTAGT | 1 cut(s) | 706 |
| AjnI | CCWGG | 4 cut(s) | 266, 273, 311, 1558 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 939, 971, 1771, 1803, 2238, 2270 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1857, 1889 |
| Alw21I | GWGCWC | 3 cut(s) | 201, 1089, 1243 |
| Alw26I | GTCTC | 2 cut(s) | 2297, 2334 |
| Alw44I | GTGCAC | 1 cut(s) | 1239 |
| AlwI | GGATC | 8 cut(s) | 341, 354, 936, 1254, 1469, 2014, 2280, 2619 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 3120 |
| Ama87I | CYCGRG | 2 cut(s) | 1979, 2377 |
| Aor13HI | TCCGGA | 1 cut(s) | 2351 |
| AoxI | GGCC | 7 cut(s) | 3, 462, 604, 1171, 2257, 2982, 3253 |
| ApaLI | GTGCAC | 1 cut(s) | 1239 |
| ApeKI | GCWGC | 6 cut(s) | 12, 223, 2142, 2874, 3117, 3543 |
| AseI | ATTAAT | 1 cut(s) | 413 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 598, 1131, 2962 |
| AspLEI | GCGC | 3 cut(s) | 207, 209, 2499 |
| AspS9I | GGNCC | 3 cut(s) | 1393, 3227, 3497 |
| AsuC2I | CCSGG | 3 cut(s) | 373, 2378, 2379 |
| AsuHPI | GGTGA | 5 cut(s) | 92, 553, 1574, 1684, 3258 |
| AsuII | TTCGAA | 1 cut(s) | 3324 |
| AsuNHI | GCTAGC | 1 cut(s) | 2720 |
| AvaI | CYCGRG | 2 cut(s) | 1979, 2377 |
| AvaII | GGWCC | 3 cut(s) | 1393, 3227, 3497 |
| AxyI | CCTNAGG | 1 cut(s) | 1122 |
| BaeGI | GKGCMC | 1 cut(s) | 1243 |
| BalI | TGGCCA | 2 cut(s) | 464, 2984 |
| BamHI | GGATCC | 1 cut(s) | 346 |
| BanI | GGYRCC | 1 cut(s) | 270 |
| BanII | GRGCYC | 3 cut(s) | 201, 1089, 3400 |
| BauI | CACGAG | 1 cut(s) | 3294 |
| BbsI | GAAGAC | 4 cut(s) | 695, 2126, 2294, 2568 |
| Bbv12I | GWGCWC | 3 cut(s) | 201, 1089, 1243 |
| BbvCI | CCTCAGC | 1 cut(s) | 2553 |
| BbvI | GCAGC | 6 cut(s) | 24, 235, 2129, 2886, 3104, 3530 |
| BccI | CCATC | 9 cut(s) | 239, 735, 1298, 1312, 2035, 2204, 2380, 2806, 2869 |
| BceAI | ACGGC | 1 cut(s) | 3352 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2863, 2897 |
| BciT130I | CCWGG | 4 cut(s) | 268, 275, 313, 1560 |
| BciVI | GTATCC | 3 cut(s) | 1793, 1809, 3009 |
| BcnI | CCSGG | 3 cut(s) | 373, 2378, 2379 |
| BcoDI | GTCTC | 2 cut(s) | 2297, 2334 |
| BcuI | ACTAGT | 1 cut(s) | 706 |
| BfmI | CTRYAG | 1 cut(s) | 2002 |
| BfoI | RGCGCY | 1 cut(s) | 2500 |
| BfrI | CTTAAG | 1 cut(s) | 1774 |
| BfuAI | ACCTGC | 2 cut(s) | 807, 3485 |
| BfuI | GTATCC | 3 cut(s) | 1793, 1809, 3009 |
| BglII | AGATCT | 2 cut(s) | 166, 2791 |
| BisI | GCNGC | 7 cut(s) | 13, 224, 2143, 2435, 2875, 3118, 3544 |
| BlsI | GCNGC | 7 cut(s) | 14, 225, 2144, 2436, 2876, 3119, 3545 |
| Bme1390I | CCNGG | 7 cut(s) | 268, 275, 313, 373, 1560, 2378, 2379 |
| Bme18I | GGWCC | 3 cut(s) | 1393, 3227, 3497 |
| BmeT110I | CYCGRG | 2 cut(s) | 1979, 2377 |
| BmgT120I | GGNCC | 3 cut(s) | 1393, 3227, 3497 |
| BmiI | GGNNCC | 3 cut(s) | 272, 348, 868 |
| BmrFI | CCNGG | 7 cut(s) | 268, 275, 313, 373, 1560, 2378, 2379 |
| BmrI | ACTGGG | 1 cut(s) | 626 |
| BmsI | GCATC | 6 cut(s) | 559, 628, 1491, 1503, 2345, 2720 |
| BmtI | GCTAGC | 1 cut(s) | 2724 |
| BmuI | ACTGGG | 1 cut(s) | 626 |
| BpiI | GAAGAC | 4 cut(s) | 695, 2126, 2294, 2568 |
| BpmI | CTGGAG | 3 cut(s) | 177, 2968, 3084 |
| Bpu10I | CCTNAGC | 2 cut(s) | 2541, 2553 |
| Bpu14I | TTCGAA | 1 cut(s) | 3324 |
| BpuEI | CTTGAG | 6 cut(s) | 28, 339, 629, 1103, 2015, 2441 |
| BpuMI | CCSGG | 3 cut(s) | 373, 2378, 2379 |
| BsaAI | YACGTR | 2 cut(s) | 2474, 2818 |
| BsaBI | GATNNNNATC | 2 cut(s) | 927, 2625 |
| BsaJI | CCNNGG | 5 cut(s) | 312, 2377, 2378, 2492, 2558 |
| BsaWI | WCCGGW | 2 cut(s) | 2351, 3224 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 124, 1127, 2101, 3120, 3506 |
| Bse21I | CCTNAGG | 1 cut(s) | 1122 |
| Bse3DI | GCAATG | 2 cut(s) | 696, 2031 |
| Bse8I | GATNNNNATC | 2 cut(s) | 927, 2625 |
| BseAI | TCCGGA | 1 cut(s) | 2351 |
| BseBI | CCWGG | 4 cut(s) | 268, 275, 313, 1560 |
| BseDI | CCNNGG | 5 cut(s) | 312, 2377, 2378, 2492, 2558 |
| BseGI | GGATG | 8 cut(s) | 283, 550, 859, 1205, 1927, 2027, 2360, 3221 |
| BseJI | GATNNNNATC | 2 cut(s) | 927, 2625 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 124, 1127, 2101, 3120, 3506 |
| BseMI | GCAATG | 2 cut(s) | 696, 2031 |
| BseMII | CTCAG | 6 cut(s) | 387, 1113, 1836, 2532, 2567, 2753 |
| BsePI | GCGCGC | 1 cut(s) | 205 |
| BseSI | GKGCMC | 1 cut(s) | 1243 |
| BseXI | GCAGC | 6 cut(s) | 24, 235, 2129, 2886, 3104, 3530 |
| BseYI | CCCAGC | 1 cut(s) | 3124 |
| Bsh1236I | CGCG | 2 cut(s) | 207, 209 |
| BshFI | GGCC | 7 cut(s) | 5, 464, 606, 1173, 2259, 2984, 3255 |
| BshNI | GGYRCC | 1 cut(s) | 270 |
| BsiHKAI | GWGCWC | 3 cut(s) | 201, 1089, 1243 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1979, 2377 |
| BsiSI | CCGG | 4 cut(s) | 372, 2352, 2378, 3225 |
| BslFI | GGGAC | 4 cut(s) | 193, 329, 1898, 3087 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 124, 1127, 2101, 3120, 3506 |
| BsmAI | GTCTC | 2 cut(s) | 2297, 2334 |
| BsmFI | GGGAC | 4 cut(s) | 193, 329, 1898, 3087 |
| BsmI | GAATGC | 2 cut(s) | 305, 1105 |
| BsnI | GGCC | 7 cut(s) | 5, 464, 606, 1173, 2259, 2984, 3255 |
| BsoBI | CYCGRG | 2 cut(s) | 1979, 2377 |
| Bsp119I | TTCGAA | 1 cut(s) | 3324 |
| Bsp1286I | GDGCHC | 4 cut(s) | 201, 1089, 1243, 3400 |
| Bsp13I | TCCGGA | 1 cut(s) | 2351 |
| BspACI | CCGC | 4 cut(s) | 1402, 2414, 2435, 3393 |
| BspANI | GGCC | 7 cut(s) | 5, 464, 606, 1173, 2259, 2984, 3255 |
| BspCNI | CTCAG | 6 cut(s) | 388, 1114, 1837, 2533, 2566, 2752 |
| BspEI | TCCGGA | 1 cut(s) | 2351 |
| BspFNI | CGCG | 2 cut(s) | 207, 209 |
| BspLI | GGNNCC | 3 cut(s) | 272, 348, 868 |
| BspMI | ACCTGC | 2 cut(s) | 807, 3485 |
| BspOI | GCTAGC | 1 cut(s) | 2724 |
| BspPI | GGATC | 8 cut(s) | 341, 354, 936, 1254, 1469, 2014, 2280, 2619 |
| BspQI | GCTCTTC | 1 cut(s) | 1094 |
| BspT104I | TTCGAA | 1 cut(s) | 3324 |
| BspT107I | GGYRCC | 1 cut(s) | 270 |
| BspTI | CTTAAG | 1 cut(s) | 1774 |
| BsrDI | GCAATG | 2 cut(s) | 696, 2031 |
| BssECI | CCNNGG | 5 cut(s) | 312, 2377, 2378, 2492, 2558 |
| BssHII | GCGCGC | 1 cut(s) | 205 |
| BssSI | CACGAG | 1 cut(s) | 3294 |
| BssT1I | CCWWGG | 2 cut(s) | 2492, 2558 |
| Bst2BI | CACGAG | 1 cut(s) | 3294 |
| Bst2UI | CCWGG | 4 cut(s) | 268, 275, 313, 1560 |
| Bst4CI | ACNGT | 7 cut(s) | 1143, 2169, 2422, 3305, 3315, 3469, 3530 |
| Bst6I | CTCTTC | 3 cut(s) | 828, 1094, 1662 |
| BstAFI | CTTAAG | 1 cut(s) | 1774 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 638, 2730, 3117 |
| BstBAI | YACGTR | 2 cut(s) | 2474, 2818 |
| BstBI | TTCGAA | 1 cut(s) | 3324 |
| BstC8I | GCNNGC | 7 cut(s) | 138, 207, 228, 2515, 2722, 3122, 3400 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1125 |
| BstF5I | GGATG | 8 cut(s) | 283, 550, 859, 1205, 1927, 2027, 2360, 3221 |
| BstFNI | CGCG | 2 cut(s) | 207, 209 |
| BstH2I | RGCGCY | 1 cut(s) | 2500 |
| BstHHI | GCGC | 3 cut(s) | 207, 209, 2499 |
| BstMAI | GTCTC | 2 cut(s) | 2297, 2334 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 638, 1027, 1637, 2730, 3117, 3399, 3469 |
| BstNI | CCWGG | 4 cut(s) | 268, 275, 313, 1560 |
| BstNSI | RCATGY | 3 cut(s) | 1350, 1433, 3372 |
| BstSCI | CCNGG | 7 cut(s) | 266, 273, 311, 371, 1558, 2376, 2377 |
| BstSFI | CTRYAG | 1 cut(s) | 2002 |
| BstSLI | GKGCMC | 1 cut(s) | 1243 |
| BstUI | CGCG | 2 cut(s) | 207, 209 |
| BstV1I | GCAGC | 6 cut(s) | 24, 235, 2129, 2886, 3104, 3530 |
| BstV2I | GAAGAC | 4 cut(s) | 695, 2126, 2294, 2568 |
| BstX2I | RGATCY | 6 cut(s) | 166, 346, 2019, 2272, 2611, 2791 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2499 |
| BstYI | RGATCY | 6 cut(s) | 166, 346, 2019, 2272, 2611, 2791 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1122 |
| BsuI | GTATCC | 3 cut(s) | 1793, 1809, 3009 |
| BsuRI | GGCC | 7 cut(s) | 5, 464, 606, 1173, 2259, 2984, 3255 |
| BtsCI | GGATG | 8 cut(s) | 283, 550, 859, 1205, 1927, 2027, 2360, 3221 |
| BtsI | GCAGTG | 1 cut(s) | 443 |
| BtsIMutI | CAGTG | 6 cut(s) | 393, 443, 1241, 2914, 3446, 3474 |
| BveI | ACCTGC | 2 cut(s) | 807, 3485 |
| Cac8I | GCNNGC | 7 cut(s) | 138, 207, 228, 2515, 2722, 3122, 3400 |
| CaiI | CAGNNNCTG | 1 cut(s) | 3120 |
| CfoI | GCGC | 3 cut(s) | 207, 209, 2499 |
| Cfr13I | GGNCC | 3 cut(s) | 1393, 3227, 3497 |
| Cfr9I | CCCGGG | 1 cut(s) | 2377 |
| CpoI | CGGWCCG | 1 cut(s) | 3227 |
| CsiI | ACCWGGT | 1 cut(s) | 266 |
| Csp6I | GTAC | 8 cut(s) | 359, 623, 786, 1950, 2267, 2835, 3010, 3409 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 276, 311, 454, 489 |
| CspI | CGGWCCG | 1 cut(s) | 3227 |
| CviQI | GTAC | 8 cut(s) | 359, 623, 786, 1950, 2267, 2835, 3010, 3409 |
| DraI | TTTAAA | 1 cut(s) | 2427 |
| DraIII | CACNNNGTG | 1 cut(s) | 270 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2191 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2191 |
| EaeI | YGGCCR | 2 cut(s) | 462, 2982 |
| Eam1104I | CTCTTC | 3 cut(s) | 828, 1094, 1662 |
| EarI | CTCTTC | 3 cut(s) | 828, 1094, 1662 |
| Ecl136II | GAGCTC | 2 cut(s) | 199, 1087 |
| Eco130I | CCWWGG | 2 cut(s) | 2492, 2558 |
| Eco147I | AGGCCT | 1 cut(s) | 1173 |
| Eco24I | GRGCYC | 3 cut(s) | 201, 1089, 3400 |
| Eco32I | GATATC | 2 cut(s) | 2644, 2656 |
| Eco47I | GGWCC | 3 cut(s) | 1393, 3227, 3497 |
| Eco53kI | GAGCTC | 2 cut(s) | 199, 1087 |
| Eco57I | CTGAAG | 2 cut(s) | 1075, 3479 |
| Eco81I | CCTNAGG | 1 cut(s) | 1122 |
| Eco88I | CYCGRG | 2 cut(s) | 1979, 2377 |
| EcoICRI | GAGCTC | 2 cut(s) | 199, 1087 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1125 |
| EcoRI | GAATTC | 3 cut(s) | 598, 1834, 3210 |
| EcoRII | CCWGG | 4 cut(s) | 266, 273, 311, 1558 |
| EcoRV | GATATC | 2 cut(s) | 2644, 2656 |
| EcoT14I | CCWWGG | 2 cut(s) | 2492, 2558 |
| EcoT22I | ATGCAT | 3 cut(s) | 305, 1506, 1707 |
| EcoT38I | GRGCYC | 3 cut(s) | 201, 1089, 3400 |
| ErhI | CCWWGG | 2 cut(s) | 2492, 2558 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 872, 904, 2421, 2453 |
| FaqI | GGGAC | 4 cut(s) | 193, 329, 1898, 3087 |
| FauI | CCCGC | 3 cut(s) | 1409, 2407, 3386 |
| FblI | GTMKAC | 2 cut(s) | 1855, 1969 |
| Fnu4HI | GCNGC | 7 cut(s) | 13, 224, 2143, 2435, 2875, 3118, 3544 |
| FokI | GGATG | 8 cut(s) | 290, 537, 866, 1212, 1934, 2014, 2367, 3208 |
| FriOI | GRGCYC | 3 cut(s) | 201, 1089, 3400 |
| Fsp4HI | GCNGC | 7 cut(s) | 13, 224, 2143, 2435, 2875, 3118, 3544 |
| GlaI | GCGC | 3 cut(s) | 206, 208, 2498 |
| GluI | GCNGC | 7 cut(s) | 13, 224, 2143, 2435, 2875, 3118, 3544 |
| GsaI | CCCAGC | 1 cut(s) | 3128 |
| GsuI | CTGGAG | 3 cut(s) | 177, 2968, 3084 |
| HaeII | RGCGCY | 1 cut(s) | 2500 |
| HaeIII | GGCC | 7 cut(s) | 5, 464, 606, 1173, 2259, 2984, 3255 |
| HapII | CCGG | 4 cut(s) | 372, 2352, 2378, 3225 |
| HhaI | GCGC | 3 cut(s) | 207, 209, 2499 |
| Hin6I | GCGC | 3 cut(s) | 205, 207, 2497 |
| HinP1I | GCGC | 3 cut(s) | 205, 207, 2497 |
| HincII | GTYRAC | 2 cut(s) | 796, 1455 |
| HindII | GTYRAC | 2 cut(s) | 796, 1455 |
| HpaI | GTTAAC | 1 cut(s) | 1455 |
| HpaII | CCGG | 4 cut(s) | 372, 2352, 2378, 3225 |
| HphI | GGTGA | 5 cut(s) | 92, 553, 1574, 1684, 3258 |
| Hpy188I | TCNGA | 8 cut(s) | 31, 593, 1098, 1936, 2307, 3209, 3366, 3501 |
| Hpy99I | CGWCG | 1 cut(s) | 48 |
| HpyCH4III | ACNGT | 7 cut(s) | 1143, 2169, 2422, 3305, 3315, 3469, 3530 |
| HpyCH4IV | ACGT | 3 cut(s) | 1015, 2473, 2817 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 638, 1027, 1637, 2730, 3117, 3399, 3469 |
| HpySE526I | ACGT | 3 cut(s) | 1015, 2473, 2817 |
| HspAI | GCGC | 3 cut(s) | 205, 207, 2497 |
| Kpn2I | TCCGGA | 1 cut(s) | 2351 |
| KspAI | GTTAAC | 1 cut(s) | 1455 |
| LguI | GCTCTTC | 1 cut(s) | 1094 |
| LmnI | GCTCC | 4 cut(s) | 433, 1372, 1628, 3091 |
| Lsp1109I | GCAGC | 6 cut(s) | 24, 235, 2129, 2886, 3104, 3530 |
| LweI | GCATC | 6 cut(s) | 559, 628, 1491, 1503, 2345, 2720 |
| MabI | ACCWGGT | 1 cut(s) | 266 |
| MaeII | ACGT | 3 cut(s) | 1015, 2473, 2817 |
| MaeIII | GTNAC | 6 cut(s) | 1928, 2279, 2460, 3264, 3296, 3485 |
| MfeI | CAATTG | 3 cut(s) | 1632, 2241, 2481 |
| MflI | RGATCY | 6 cut(s) | 166, 346, 2019, 2272, 2611, 2791 |
| MhlI | GDGCHC | 4 cut(s) | 201, 1089, 1243, 3400 |
| MlsI | TGGCCA | 2 cut(s) | 464, 2984 |
| MluNI | TGGCCA | 2 cut(s) | 464, 2984 |
| MlyI | GAGTC | 2 cut(s) | 1556, 2545 |
| MmeI | TCCRAC | 4 cut(s) | 29, 571, 937, 3215 |
| Mox20I | TGGCCA | 2 cut(s) | 464, 2984 |
| Mph1103I | ATGCAT | 3 cut(s) | 305, 1506, 1707 |
| MroI | TCCGGA | 1 cut(s) | 2351 |
| MroXI | GAANNNNTTC | 3 cut(s) | 598, 1131, 2962 |
| MscI | TGGCCA | 2 cut(s) | 464, 2984 |
| MslI | CAYNNNNRTG | 1 cut(s) | 2820 |
| Msp20I | TGGCCA | 2 cut(s) | 464, 2984 |
| MspA1I | CMGCKG | 1 cut(s) | 3117 |
| MspCI | CTTAAG | 1 cut(s) | 1774 |
| MspI | CCGG | 4 cut(s) | 372, 2352, 2378, 3225 |
| MspR9I | CCNGG | 7 cut(s) | 268, 275, 313, 373, 1560, 2378, 2379 |
| MunI | CAATTG | 3 cut(s) | 1632, 2241, 2481 |
| Mva1269I | GAATGC | 2 cut(s) | 305, 1105 |
| MvaI | CCWGG | 4 cut(s) | 268, 275, 313, 1560 |
| MvnI | CGCG | 2 cut(s) | 207, 209 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 638, 1027, 1637, 2730, 3117, 3399, 3469 |
| NciI | CCSGG | 3 cut(s) | 373, 2378, 2379 |
| NheI | GCTAGC | 1 cut(s) | 2720 |
| NlaIV | GGNNCC | 3 cut(s) | 272, 348, 868 |
| NmuCI | GTSAC | 4 cut(s) | 2279, 3264, 3296, 3485 |
| NsiI | ATGCAT | 3 cut(s) | 305, 1506, 1707 |
| NspI | RCATGY | 3 cut(s) | 1350, 1433, 3372 |
| NspV | TTCGAA | 1 cut(s) | 3324 |
| PaeR7I | CTCGAG | 1 cut(s) | 1979 |
| PauI | GCGCGC | 1 cut(s) | 205 |
| PceI | AGGCCT | 1 cut(s) | 1173 |
| PciI | ACATGT | 1 cut(s) | 1429 |
| PciSI | GCTCTTC | 1 cut(s) | 1094 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 531, 2814 |
| PctI | GAATGC | 2 cut(s) | 305, 1105 |
| PdmI | GAANNNNTTC | 3 cut(s) | 598, 1131, 2962 |
| PflFI | GACNNNGTC | 1 cut(s) | 524 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 124, 3120 |
| PkrI | GCNGC | 7 cut(s) | 14, 225, 2144, 2436, 2876, 3119, 3545 |
| PleI | GAGTC | 2 cut(s) | 1555, 2544 |
| PpsI | GAGTC | 2 cut(s) | 1555, 2544 |
| Ppu21I | YACGTR | 2 cut(s) | 2474, 2818 |
| PscI | ACATGT | 1 cut(s) | 1429 |
| PshBI | ATTAAT | 1 cut(s) | 413 |
| Psp124BI | GAGCTC | 2 cut(s) | 201, 1089 |
| Psp1406I | AACGTT | 1 cut(s) | 1015 |
| Psp6I | CCWGG | 4 cut(s) | 266, 273, 311, 1558 |
| PspFI | CCCAGC | 1 cut(s) | 3124 |
| PspGI | CCWGG | 4 cut(s) | 266, 273, 311, 1558 |
| PspN4I | GGNNCC | 3 cut(s) | 272, 348, 868 |
| PspPI | GGNCC | 3 cut(s) | 1393, 3227, 3497 |
| PstNI | CAGNNNCTG | 1 cut(s) | 3120 |
| PsuI | RGATCY | 6 cut(s) | 166, 346, 2019, 2272, 2611, 2791 |
| PsyI | GACNNNGTC | 1 cut(s) | 524 |
| PteI | GCGCGC | 1 cut(s) | 205 |
| PvuII | CAGCTG | 1 cut(s) | 3117 |
| RsaI | GTAC | 8 cut(s) | 360, 624, 787, 1951, 2268, 2836, 3011, 3410 |
| RsaNI | GTAC | 8 cut(s) | 359, 623, 786, 1950, 2267, 2835, 3010, 3409 |
| RseI | CAYNNNNRTG | 1 cut(s) | 2820 |
| Rsr2I | CGGWCCG | 1 cut(s) | 3227 |
| RsrII | CGGWCCG | 1 cut(s) | 3227 |
| SacI | GAGCTC | 2 cut(s) | 201, 1089 |
| SapI | GCTCTTC | 1 cut(s) | 1094 |
| SatI | GCNGC | 7 cut(s) | 13, 224, 2143, 2435, 2875, 3118, 3544 |
| Sau96I | GGNCC | 3 cut(s) | 1393, 3227, 3497 |
| SchI | GAGTC | 2 cut(s) | 1556, 2545 |
| ScrFI | CCNGG | 7 cut(s) | 268, 275, 313, 373, 1560, 2378, 2379 |
| SduI | GDGCHC | 4 cut(s) | 201, 1089, 1243, 3400 |
| SexAI | ACCWGGT | 1 cut(s) | 266 |
| SfaNI | GCATC | 6 cut(s) | 559, 628, 1491, 1503, 2345, 2720 |
| SfcI | CTRYAG | 1 cut(s) | 2002 |
| Sfr274I | CTCGAG | 1 cut(s) | 1979 |
| SfuI | TTCGAA | 1 cut(s) | 3324 |
| SinI | GGWCC | 3 cut(s) | 1393, 3227, 3497 |
| SlaI | CTCGAG | 1 cut(s) | 1979 |
| SmaI | CCCGGG | 1 cut(s) | 2379 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 2820 |
| SmlI | CTYRAG | 8 cut(s) | 7, 354, 608, 1082, 1774, 1979, 1994, 2456 |
| SmoI | CTYRAG | 8 cut(s) | 7, 354, 608, 1082, 1774, 1979, 1994, 2456 |
| SpeI | ACTAGT | 1 cut(s) | 706 |
| SseBI | AGGCCT | 1 cut(s) | 1173 |
| SsiI | CCGC | 4 cut(s) | 1402, 2414, 2435, 3393 |
| SspI | AATATT | 3 cut(s) | 951, 1278, 2126 |
| SstI | GAGCTC | 2 cut(s) | 201, 1089 |
| StuI | AGGCCT | 1 cut(s) | 1173 |
| StyD4I | CCNGG | 7 cut(s) | 266, 273, 311, 371, 1558, 2376, 2377 |
| StyI | CCWWGG | 2 cut(s) | 2492, 2558 |
| TaaI | ACNGT | 7 cut(s) | 1143, 2169, 2422, 3305, 3315, 3469, 3530 |
| TaiI | ACGT | 3 cut(s) | 1018, 2476, 2820 |
| TaqI | TCGA | 4 cut(s) | 1488, 1722, 1980, 3324 |
| TatI | WGTACW | 3 cut(s) | 622, 2266, 2834 |
| TauI | GCSGC | 1 cut(s) | 2437 |
| TscAI | CASTG | 6 cut(s) | 400, 443, 1248, 2914, 3453, 3474 |
| TseFI | GTSAC | 4 cut(s) | 2279, 3264, 3296, 3485 |
| TseI | GCWGC | 6 cut(s) | 12, 223, 2142, 2874, 3117, 3543 |
| Tsp45I | GTSAC | 4 cut(s) | 2279, 3264, 3296, 3485 |
| TspGWI | ACGGA | 2 cut(s) | 1902, 3219 |
| TspMI | CCCGGG | 1 cut(s) | 2377 |
| TspRI | CASTG | 6 cut(s) | 400, 443, 1248, 2914, 3453, 3474 |
| Tth111I | GACNNNGTC | 1 cut(s) | 524 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 124, 3120 |
| Vha464I | CTTAAG | 1 cut(s) | 1774 |
| VneI | GTGCAC | 1 cut(s) | 1239 |
| VpaK11BI | GGWCC | 3 cut(s) | 1393, 3227, 3497 |
| VspI | ATTAAT | 1 cut(s) | 413 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1125 |
| XbaI | TCTAGA | 1 cut(s) | 3344 |
| XceI | RCATGY | 3 cut(s) | 1350, 1433, 3372 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 271, 3121 |
| XhoI | CTCGAG | 1 cut(s) | 1979 |
| XmaI | CCCGGG | 1 cut(s) | 2377 |
| XmiI | GTMKAC | 2 cut(s) | 1855, 1969 |
| XmnI | GAANNNNTTC | 3 cut(s) | 598, 1131, 2962 |
| Zsp2I | ATGCAT | 3 cut(s) | 305, 1506, 1707 |