TMV resistance protein N-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1181 |
| AatII | GACGTC | 1 cut(s) | 185 |
| Acc16I | TGCGCA | 1 cut(s) | 1997 |
| Acc36I | ACCTGC | 2 cut(s) | 547, 2275 |
| Acc65I | GGTACC | 1 cut(s) | 2287 |
| AccB1I | GGYRCC | 1 cut(s) | 2287 |
| AccI | GTMKAC | 2 cut(s) | 2156, 2627 |
| AccII | CGCG | 4 cut(s) | 1943, 2147, 2396, 3227 |
| AccIII | TCCGGA | 2 cut(s) | 2620, 2644 |
| AcoI | YGGCCR | 1 cut(s) | 3219 |
| AcuI | CTGAAG | 2 cut(s) | 1136, 2484 |
| AcyI | GRCGYC | 2 cut(s) | 182, 2387 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 545, 781, 2171, 2678 |
| AflII | CTTAAG | 2 cut(s) | 605, 2743 |
| AflIII | ACRYGT | 1 cut(s) | 1928 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 935 |
| AjnI | CCWGG | 7 cut(s) | 109, 961, 2326, 2341, 2524, 2824, 3209 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 738, 770, 1593, 1625 |
| AleI | CACNNNNGTG | 2 cut(s) | 2589, 3078 |
| Alw26I | GTCTC | 5 cut(s) | 454, 1218, 2248, 2364, 2374 |
| Ama87I | CYCGRG | 1 cut(s) | 2473 |
| Aor13HI | TCCGGA | 2 cut(s) | 2620, 2644 |
| AoxI | GGCC | 6 cut(s) | 84, 589, 1153, 2459, 3021, 3219 |
| ApeKI | GCWGC | 5 cut(s) | 1545, 1737, 2034, 2037, 3147 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2648, 2680 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 228, 1245, 1703 |
| Asp718I | GGTACC | 1 cut(s) | 2287 |
| AspLEI | GCGC | 1 cut(s) | 1998 |
| AspS9I | GGNCC | 7 cut(s) | 589, 1154, 1183, 2459, 2669, 3127, 3181 |
| AsuHPI | GGTGA | 8 cut(s) | 1661, 1760, 2391, 2498, 2576, 2701, 3065, 3178 |
| AsuII | TTCGAA | 1 cut(s) | 2850 |
| AvaI | CYCGRG | 1 cut(s) | 2473 |
| AvaII | GGWCC | 4 cut(s) | 1183, 2669, 3127, 3181 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 3037, 3070 |
| BalI | TGGCCA | 1 cut(s) | 3221 |
| BamHI | GGATCC | 3 cut(s) | 43, 2045, 2641 |
| BanI | GGYRCC | 1 cut(s) | 2287 |
| BbsI | GAAGAC | 6 cut(s) | 934, 1157, 1456, 1487, 2064, 2877 |
| BbvI | GCAGC | 5 cut(s) | 1532, 1724, 2021, 2049, 3159 |
| BccI | CCATC | 7 cut(s) | 275, 719, 1213, 1438, 1921, 2168, 2914 |
| BceAI | ACGGC | 1 cut(s) | 1772 |
| BcgI | CGANNNNNNTGC | 8 cut(s) | 394, 428, 479, 513, 956, 990, 2413, 2447 |
| BciT130I | CCWGG | 7 cut(s) | 111, 963, 2328, 2343, 2526, 2826, 3211 |
| BciVI | GTATCC | 1 cut(s) | 1212 |
| BclI | TGATCA | 2 cut(s) | 688, 1276 |
| BcoDI | GTCTC | 5 cut(s) | 454, 1218, 2248, 2364, 2374 |
| BfaI | CTAG | 5 cut(s) | 329, 440, 812, 1745, 2856 |
| BfmI | CTRYAG | 4 cut(s) | 540, 1738, 2035, 2225 |
| BfrI | CTTAAG | 2 cut(s) | 605, 2743 |
| BfuAI | ACCTGC | 2 cut(s) | 547, 2275 |
| BfuI | GTATCC | 1 cut(s) | 1212 |
| BglII | AGATCT | 2 cut(s) | 240, 3269 |
| BisI | GCNGC | 8 cut(s) | 1546, 1738, 2035, 2038, 2117, 2132, 2394, 3148 |
| BlpI | GCTNAGC | 1 cut(s) | 666 |
| BlsI | GCNGC | 8 cut(s) | 1547, 1739, 2036, 2039, 2118, 2133, 2395, 3149 |
| BmcAI | AGTACT | 2 cut(s) | 2317, 2815 |
| Bme1390I | CCNGG | 7 cut(s) | 111, 963, 2328, 2343, 2526, 2826, 3211 |
| Bme18I | GGWCC | 4 cut(s) | 1183, 2669, 3127, 3181 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 935 |
| BmeT110I | CYCGRG | 1 cut(s) | 2473 |
| BmgT120I | GGNCC | 7 cut(s) | 589, 1154, 1183, 2459, 2669, 3127, 3181 |
| BmrFI | CCNGG | 7 cut(s) | 111, 963, 2328, 2343, 2526, 2826, 3211 |
| BmsI | GCATC | 3 cut(s) | 136, 1270, 3142 |
| BoxI | GACNNNNGTC | 1 cut(s) | 2386 |
| BpiI | GAAGAC | 6 cut(s) | 934, 1157, 1456, 1487, 2064, 2877 |
| BpmI | CTGGAG | 1 cut(s) | 396 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 666 |
| Bpu14I | TTCGAA | 1 cut(s) | 2850 |
| BpuEI | CTTGAG | 4 cut(s) | 761, 1109, 1403, 1844 |
| BsaAI | YACGTR | 1 cut(s) | 2916 |
| BsaBI | GATNNNNATC | 1 cut(s) | 345 |
| BsaHI | GRCGYC | 2 cut(s) | 182, 2387 |
| BsaI | GGTCTC | 1 cut(s) | 2248 |
| BsaJI | CCNNGG | 7 cut(s) | 110, 585, 962, 1941, 2091, 2342, 3209 |
| BsaWI | WCCGGW | 4 cut(s) | 1681, 2620, 2644, 3249 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 2087, 2117, 2166, 2196, 3231, 3261 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 545, 781, 2171, 2678 |
| Bse118I | RCCGGY | 1 cut(s) | 1234 |
| Bse1I | ACTGG | 3 cut(s) | 366, 392, 2544 |
| Bse8I | GATNNNNATC | 1 cut(s) | 345 |
| BseAI | TCCGGA | 2 cut(s) | 2620, 2644 |
| BseBI | CCWGG | 7 cut(s) | 111, 963, 2328, 2343, 2526, 2826, 3211 |
| BseDI | CCNNGG | 7 cut(s) | 110, 585, 962, 1941, 2091, 2342, 3209 |
| BseGI | GGATG | 4 cut(s) | 790, 1426, 2160, 2925 |
| BseJI | GATNNNNATC | 1 cut(s) | 345 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 545, 781, 2171, 2678 |
| BseMII | CTCAG | 8 cut(s) | 724, 1329, 1380, 1459, 1667, 2388, 2691, 2889 |
| BseNI | ACTGG | 3 cut(s) | 366, 392, 2544 |
| BseXI | GCAGC | 5 cut(s) | 1532, 1724, 2021, 2049, 3159 |
| BsgI | GTGCAG | 3 cut(s) | 678, 2303, 2801 |
| Bsh1236I | CGCG | 4 cut(s) | 1943, 2147, 2396, 3227 |
| Bsh1285I | CGRYCG | 1 cut(s) | 892 |
| BshFI | GGCC | 6 cut(s) | 86, 591, 1155, 2461, 3023, 3221 |
| BshNI | GGYRCC | 1 cut(s) | 2287 |
| BsiEI | CGRYCG | 1 cut(s) | 892 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2473 |
| BsiSI | CCGG | 5 cut(s) | 1235, 1682, 2621, 2645, 3250 |
| BslFI | GGGAC | 2 cut(s) | 1339, 2156 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 545, 781, 2171, 2678 |
| BsmAI | GTCTC | 5 cut(s) | 454, 1218, 2248, 2364, 2374 |
| BsmFI | GGGAC | 2 cut(s) | 1339, 2156 |
| BsmI | GAATGC | 1 cut(s) | 2769 |
| BsnI | GGCC | 6 cut(s) | 86, 591, 1155, 2461, 3023, 3221 |
| Bso31I | GGTCTC | 1 cut(s) | 2248 |
| BsoBI | CYCGRG | 1 cut(s) | 2473 |
| Bsp119I | TTCGAA | 1 cut(s) | 2850 |
| Bsp13I | TCCGGA | 2 cut(s) | 2620, 2644 |
| Bsp1407I | TGTACA | 2 cut(s) | 1406, 2715 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 666 |
| Bsp19I | CCATGG | 1 cut(s) | 2091 |
| BspANI | GGCC | 6 cut(s) | 86, 591, 1155, 2461, 3023, 3221 |
| BspCNI | CTCAG | 8 cut(s) | 723, 1330, 1379, 1458, 1668, 2387, 2690, 2888 |
| BspEI | TCCGGA | 2 cut(s) | 2620, 2644 |
| BspFNI | CGCG | 4 cut(s) | 1943, 2147, 2396, 3227 |
| BspHI | TCATGA | 3 cut(s) | 843, 2268, 3275 |
| BspMAI | CTGCAG | 3 cut(s) | 544, 1742, 2039 |
| BspMI | ACCTGC | 2 cut(s) | 547, 2275 |
| BspT104I | TTCGAA | 1 cut(s) | 2850 |
| BspT107I | GGYRCC | 1 cut(s) | 2287 |
| BspTI | CTTAAG | 2 cut(s) | 605, 2743 |
| BspTNI | GGTCTC | 1 cut(s) | 2248 |
| BsrFI | RCCGGY | 1 cut(s) | 1234 |
| BsrGI | TGTACA | 2 cut(s) | 1406, 2715 |
| BsrI | ACTGG | 3 cut(s) | 366, 392, 2544 |
| BssAI | RCCGGY | 1 cut(s) | 1234 |
| BssECI | CCNNGG | 7 cut(s) | 110, 585, 962, 1941, 2091, 2342, 3209 |
| BssNAI | GTATAC | 1 cut(s) | 2628 |
| BssNI | GRCGYC | 2 cut(s) | 182, 2387 |
| BssT1I | CCWWGG | 2 cut(s) | 585, 2091 |
| Bst1107I | GTATAC | 1 cut(s) | 2628 |
| Bst2UI | CCWGG | 7 cut(s) | 111, 963, 2328, 2343, 2526, 2826, 3211 |
| Bst4CI | ACNGT | 6 cut(s) | 561, 893, 2195, 2320, 2818, 3050 |
| Bst6I | CTCTTC | 2 cut(s) | 1065, 1221 |
| BstACI | GRCGYC | 2 cut(s) | 182, 2387 |
| BstAFI | CTTAAG | 2 cut(s) | 605, 2743 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 3205 |
| BstAUI | TGTACA | 2 cut(s) | 1406, 2715 |
| BstBAI | YACGTR | 1 cut(s) | 2916 |
| BstBI | TTCGAA | 1 cut(s) | 2850 |
| BstC8I | GCNNGC | 5 cut(s) | 175, 1058, 1625, 1837, 2363 |
| BstDSI | CCRYGG | 2 cut(s) | 1941, 2091 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 543 |
| BstF5I | GGATG | 4 cut(s) | 790, 1426, 2160, 2925 |
| BstFNI | CGCG | 4 cut(s) | 1943, 2147, 2396, 3227 |
| BstHHI | GCGC | 1 cut(s) | 1998 |
| BstMAI | GTCTC | 5 cut(s) | 454, 1218, 2248, 2364, 2374 |
| BstMCI | CGRYCG | 1 cut(s) | 892 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 170, 1258, 1855, 2122, 2131, 2384, 2393, 3205 |
| BstNI | CCWGG | 7 cut(s) | 111, 963, 2328, 2343, 2526, 2826, 3211 |
| BstNSI | RCATGY | 4 cut(s) | 771, 1627, 1932, 2365 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 2386 |
| BstSCI | CCNGG | 7 cut(s) | 109, 961, 2326, 2341, 2524, 2824, 3209 |
| BstSFI | CTRYAG | 4 cut(s) | 540, 1738, 2035, 2225 |
| BstUI | CGCG | 4 cut(s) | 1943, 2147, 2396, 3227 |
| BstV1I | GCAGC | 5 cut(s) | 1532, 1724, 2021, 2049, 3159 |
| BstV2I | GAAGAC | 6 cut(s) | 934, 1157, 1456, 1487, 2064, 2877 |
| BstX2I | RGATCY | 8 cut(s) | 43, 240, 617, 1801, 1981, 2045, 2641, 3269 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 2762, 3217 |
| BstYI | RGATCY | 8 cut(s) | 43, 240, 617, 1801, 1981, 2045, 2641, 3269 |
| BstZ17I | GTATAC | 1 cut(s) | 2628 |
| BsuI | GTATCC | 1 cut(s) | 1212 |
| BsuRI | GGCC | 6 cut(s) | 86, 591, 1155, 2461, 3023, 3221 |
| BtgI | CCRYGG | 2 cut(s) | 1941, 2091 |
| BtgZI | GCGATG | 1 cut(s) | 3165 |
| BtsCI | GGATG | 4 cut(s) | 790, 1426, 2160, 2925 |
| BtsIMutI | CAGTG | 3 cut(s) | 1673, 2200, 2883 |
| BveI | ACCTGC | 2 cut(s) | 547, 2275 |
| Cac8I | GCNNGC | 5 cut(s) | 175, 1058, 1625, 1837, 2363 |
| CciI | TCATGA | 3 cut(s) | 843, 2268, 3275 |
| CfoI | GCGC | 1 cut(s) | 1998 |
| Cfr10I | RCCGGY | 1 cut(s) | 1234 |
| Cfr13I | GGNCC | 7 cut(s) | 589, 1154, 1183, 2459, 2669, 3127, 3181 |
| Cfr42I | CCGCGG | 1 cut(s) | 1944 |
| CseI | GACGC | 3 cut(s) | 2153, 2376, 2385 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1974, 2009 |
| DraI | TTTAAA | 2 cut(s) | 1869, 2556 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1181 |
| DriI | GACNNNNNGTC | 1 cut(s) | 935 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1181 |
| EaeI | YGGCCR | 1 cut(s) | 3219 |
| Eam1104I | CTCTTC | 2 cut(s) | 1065, 1221 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 935 |
| EarI | CTCTTC | 2 cut(s) | 1065, 1221 |
| EciI | GGCGGA | 3 cut(s) | 1774, 2112, 2118 |
| Eco130I | CCWWGG | 2 cut(s) | 585, 2091 |
| Eco147I | AGGCCT | 1 cut(s) | 86 |
| Eco31I | GGTCTC | 1 cut(s) | 2248 |
| Eco47I | GGWCC | 4 cut(s) | 1183, 2669, 3127, 3181 |
| Eco57I | CTGAAG | 2 cut(s) | 1136, 2484 |
| Eco88I | CYCGRG | 1 cut(s) | 2473 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 543 |
| EcoO109I | RGGNCCY | 3 cut(s) | 589, 1154, 1183 |
| EcoRI | GAATTC | 1 cut(s) | 2648 |
| EcoRII | CCWGG | 7 cut(s) | 109, 961, 2326, 2341, 2524, 2824, 3209 |
| EcoT14I | CCWWGG | 2 cut(s) | 585, 2091 |
| EcoT22I | ATGCAT | 2 cut(s) | 906, 3157 |
| ErhI | CCWWGG | 2 cut(s) | 585, 2091 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 213, 245 |
| FaqI | GGGAC | 2 cut(s) | 1339, 2156 |
| FauI | CCCGC | 2 cut(s) | 1015, 2548 |
| FauNDI | CATATG | 1 cut(s) | 1101 |
| FbaI | TGATCA | 2 cut(s) | 688, 1276 |
| FblI | GTMKAC | 2 cut(s) | 2156, 2627 |
| Fnu4HI | GCNGC | 8 cut(s) | 1546, 1738, 2035, 2038, 2117, 2132, 2394, 3148 |
| FokI | GGATG | 4 cut(s) | 797, 1413, 2147, 2932 |
| Fsp4HI | GCNGC | 8 cut(s) | 1546, 1738, 2035, 2038, 2117, 2132, 2394, 3148 |
| FspBI | CTAG | 5 cut(s) | 329, 440, 812, 1745, 2856 |
| FspI | TGCGCA | 1 cut(s) | 1997 |
| GlaI | GCGC | 1 cut(s) | 1997 |
| GluI | GCNGC | 8 cut(s) | 1546, 1738, 2035, 2038, 2117, 2132, 2394, 3148 |
| GsuI | CTGGAG | 1 cut(s) | 396 |
| HaeIII | GGCC | 6 cut(s) | 86, 591, 1155, 2461, 3023, 3221 |
| HapII | CCGG | 5 cut(s) | 1235, 1682, 2621, 2645, 3250 |
| HgaI | GACGC | 3 cut(s) | 2153, 2376, 2385 |
| HhaI | GCGC | 1 cut(s) | 1998 |
| Hin1I | GRCGYC | 2 cut(s) | 182, 2387 |
| Hin6I | GCGC | 1 cut(s) | 1996 |
| HinP1I | GCGC | 1 cut(s) | 1996 |
| HincII | GTYRAC | 3 cut(s) | 876, 1314, 1813 |
| HindII | GTYRAC | 3 cut(s) | 876, 1314, 1813 |
| HpaI | GTTAAC | 3 cut(s) | 876, 1314, 1813 |
| HpaII | CCGG | 5 cut(s) | 1235, 1682, 2621, 2645, 3250 |
| HphI | GGTGA | 8 cut(s) | 1661, 1760, 2391, 2498, 2576, 2701, 3065, 3178 |
| Hpy166II | GTNNAC | 6 cut(s) | 876, 1314, 1813, 2157, 2628, 3130 |
| Hpy8I | GTNNAC | 6 cut(s) | 876, 1314, 1813, 2157, 2628, 3130 |
| HpyCH4III | ACNGT | 6 cut(s) | 561, 893, 2195, 2320, 2818, 3050 |
| HpyCH4IV | ACGT | 2 cut(s) | 182, 2915 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 170, 1258, 1855, 2122, 2131, 2384, 2393, 3205 |
| HpySE526I | ACGT | 2 cut(s) | 182, 2915 |
| Hsp92I | GRCGYC | 2 cut(s) | 182, 2387 |
| HspAI | GCGC | 1 cut(s) | 1996 |
| Kpn2I | TCCGGA | 2 cut(s) | 2620, 2644 |
| KpnI | GGTACC | 1 cut(s) | 2291 |
| Ksp22I | TGATCA | 2 cut(s) | 688, 1276 |
| KspAI | GTTAAC | 3 cut(s) | 876, 1314, 1813 |
| KspI | CCGCGG | 1 cut(s) | 1944 |
| LmnI | GCTCC | 5 cut(s) | 72, 685, 1754, 2095, 3252 |
| Lsp1109I | GCAGC | 5 cut(s) | 1532, 1724, 2021, 2049, 3159 |
| LweI | GCATC | 3 cut(s) | 136, 1270, 3142 |
| MaeI | CTAG | 5 cut(s) | 329, 440, 812, 1745, 2856 |
| MaeII | ACGT | 2 cut(s) | 182, 2915 |
| MaeIII | GTNAC | 4 cut(s) | 415, 548, 1936, 2397 |
| MfeI | CAATTG | 3 cut(s) | 1898, 2029, 3262 |
| MflI | RGATCY | 8 cut(s) | 43, 240, 617, 1801, 1981, 2045, 2641, 3269 |
| MlsI | TGGCCA | 1 cut(s) | 3221 |
| MluNI | TGGCCA | 1 cut(s) | 3221 |
| MlyI | GAGTC | 7 cut(s) | 113, 956, 1501, 1687, 2366, 2669, 2867 |
| MmeI | TCCRAC | 3 cut(s) | 494, 543, 2996 |
| Mox20I | TGGCCA | 1 cut(s) | 3221 |
| Mph1103I | ATGCAT | 2 cut(s) | 906, 3157 |
| MroI | TCCGGA | 2 cut(s) | 2620, 2644 |
| MroXI | GAANNNNTTC | 3 cut(s) | 228, 1245, 1703 |
| MscI | TGGCCA | 1 cut(s) | 3221 |
| MslI | CAYNNNNRTG | 4 cut(s) | 2589, 2774, 2840, 3078 |
| Msp20I | TGGCCA | 1 cut(s) | 3221 |
| MspA1I | CMGCKG | 2 cut(s) | 1943, 2230 |
| MspCI | CTTAAG | 2 cut(s) | 605, 2743 |
| MspI | CCGG | 5 cut(s) | 1235, 1682, 2621, 2645, 3250 |
| MspR9I | CCNGG | 7 cut(s) | 111, 963, 2328, 2343, 2526, 2826, 3211 |
| MssI | GTTTAAAC | 1 cut(s) | 1869 |
| MunI | CAATTG | 3 cut(s) | 1898, 2029, 3262 |
| Mva1269I | GAATGC | 1 cut(s) | 2769 |
| MvaI | CCWGG | 7 cut(s) | 111, 963, 2328, 2343, 2526, 2826, 3211 |
| MvnI | CGCG | 4 cut(s) | 1943, 2147, 2396, 3227 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 170, 1258, 1855, 2122, 2131, 2384, 2393, 3205 |
| NcoI | CCATGG | 1 cut(s) | 2091 |
| NdeI | CATATG | 1 cut(s) | 1101 |
| NmuCI | GTSAC | 1 cut(s) | 2397 |
| NsbI | TGCGCA | 1 cut(s) | 1997 |
| NsiI | ATGCAT | 2 cut(s) | 906, 3157 |
| NspI | RCATGY | 4 cut(s) | 771, 1627, 1932, 2365 |
| NspV | TTCGAA | 1 cut(s) | 2850 |
| OliI | CACNNNNGTG | 2 cut(s) | 2589, 3078 |
| PaeI | GCATGC | 2 cut(s) | 1627, 2365 |
| PagI | TCATGA | 3 cut(s) | 843, 2268, 3275 |
| PceI | AGGCCT | 1 cut(s) | 86 |
| PciI | ACATGT | 1 cut(s) | 1928 |
| PctI | GAATGC | 1 cut(s) | 2769 |
| PdmI | GAANNNNTTC | 3 cut(s) | 228, 1245, 1703 |
| PfeI | GAWTC | 8 cut(s) | 1084, 1129, 1378, 1393, 1517, 1572, 2633, 2949 |
| PflFI | GACNNNGTC | 1 cut(s) | 1351 |
| PfoI | TCCNGGA | 2 cut(s) | 2326, 2824 |
| PkrI | GCNGC | 8 cut(s) | 1547, 1739, 2036, 2039, 2118, 2133, 2395, 3149 |
| PleI | GAGTC | 7 cut(s) | 113, 955, 1500, 1686, 2366, 2669, 2867 |
| PmeI | GTTTAAAC | 1 cut(s) | 1869 |
| PpsI | GAGTC | 7 cut(s) | 113, 955, 1500, 1686, 2366, 2669, 2867 |
| Ppu21I | YACGTR | 1 cut(s) | 2916 |
| PpuMI | RGGWCCY | 1 cut(s) | 1183 |
| PscI | ACATGT | 1 cut(s) | 1928 |
| PshAI | GACNNNNGTC | 1 cut(s) | 2386 |
| Psp5II | RGGWCCY | 1 cut(s) | 1183 |
| Psp6I | CCWGG | 7 cut(s) | 109, 961, 2326, 2341, 2524, 2824, 3209 |
| PspGI | CCWGG | 7 cut(s) | 109, 961, 2326, 2341, 2524, 2824, 3209 |
| PspPI | GGNCC | 7 cut(s) | 589, 1154, 1183, 2459, 2669, 3127, 3181 |
| PspPPI | RGGWCCY | 1 cut(s) | 1183 |
| PstI | CTGCAG | 3 cut(s) | 544, 1742, 2039 |
| PsuI | RGATCY | 8 cut(s) | 43, 240, 617, 1801, 1981, 2045, 2641, 3269 |
| PsyI | GACNNNGTC | 1 cut(s) | 1351 |
| RseI | CAYNNNNRTG | 4 cut(s) | 2589, 2774, 2840, 3078 |
| SacII | CCGCGG | 1 cut(s) | 1944 |
| SatI | GCNGC | 8 cut(s) | 1546, 1738, 2035, 2038, 2117, 2132, 2394, 3148 |
| Sau96I | GGNCC | 7 cut(s) | 589, 1154, 1183, 2459, 2669, 3127, 3181 |
| ScaI | AGTACT | 2 cut(s) | 2317, 2815 |
| SchI | GAGTC | 7 cut(s) | 113, 956, 1501, 1687, 2366, 2669, 2867 |
| ScrFI | CCNGG | 7 cut(s) | 111, 963, 2328, 2343, 2526, 2826, 3211 |
| SfaNI | GCATC | 3 cut(s) | 136, 1270, 3142 |
| SfcI | CTRYAG | 4 cut(s) | 540, 1738, 2035, 2225 |
| Sfr303I | CCGCGG | 1 cut(s) | 1944 |
| SfuI | TTCGAA | 1 cut(s) | 2850 |
| SgrBI | CCGCGG | 1 cut(s) | 1944 |
| SinI | GGWCC | 4 cut(s) | 1183, 2669, 3127, 3181 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 2589, 2774, 2840, 3078 |
| SmlI | CTYRAG | 6 cut(s) | 605, 776, 1124, 1382, 1859, 2743 |
| SmoI | CTYRAG | 6 cut(s) | 605, 776, 1124, 1382, 1859, 2743 |
| SphI | GCATGC | 2 cut(s) | 1627, 2365 |
| SseBI | AGGCCT | 1 cut(s) | 86 |
| SspMI | CTAG | 5 cut(s) | 329, 440, 812, 1745, 2856 |
| StuI | AGGCCT | 1 cut(s) | 86 |
| StyD4I | CCNGG | 7 cut(s) | 109, 961, 2326, 2341, 2524, 2824, 3209 |
| StyI | CCWWGG | 2 cut(s) | 585, 2091 |
| TaaI | ACNGT | 6 cut(s) | 561, 893, 2195, 2320, 2818, 3050 |
| TaiI | ACGT | 2 cut(s) | 185, 2918 |
| TaqI | TCGA | 7 cut(s) | 759, 1188, 1853, 2609, 2655, 2850, 2989 |
| TatI | WGTACW | 5 cut(s) | 1406, 2315, 2715, 2813, 2999 |
| TauI | GCSGC | 3 cut(s) | 2119, 2134, 2396 |
| TfiI | GAWTC | 8 cut(s) | 1084, 1129, 1378, 1393, 1517, 1572, 2633, 2949 |
| TscAI | CASTG | 3 cut(s) | 1680, 2200, 2883 |
| TseFI | GTSAC | 1 cut(s) | 2397 |
| TseI | GCWGC | 5 cut(s) | 1545, 1737, 2034, 2037, 3147 |
| Tsp45I | GTSAC | 1 cut(s) | 2397 |
| TspGWI | ACGGA | 7 cut(s) | 85, 585, 929, 2225, 2654, 2696, 3173 |
| TspRI | CASTG | 3 cut(s) | 1680, 2200, 2883 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1351 |
| Vha464I | CTTAAG | 2 cut(s) | 605, 2743 |
| VpaK11BI | GGWCC | 4 cut(s) | 1183, 2669, 3127, 3181 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 543 |
| XceI | RCATGY | 4 cut(s) | 771, 1627, 1932, 2365 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 107 |
| XmiI | GTMKAC | 2 cut(s) | 2156, 2627 |
| XmnI | GAANNNNTTC | 3 cut(s) | 228, 1245, 1703 |
| XspI | CTAG | 5 cut(s) | 329, 440, 812, 1745, 2856 |
| ZraI | GACGTC | 1 cut(s) | 183 |
| ZrmI | AGTACT | 2 cut(s) | 2317, 2815 |
| Zsp2I | ATGCAT | 2 cut(s) | 906, 3157 |