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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 123 |
| Acc16I | TGCGCA | 2 cut(s) | 98, 110 |
| AccI | GTMKAC | 1 cut(s) | 123 |
| AclWI | GGATC | 5 cut(s) | 150, 163, 275, 416, 512 |
| AcoI | YGGCCR | 1 cut(s) | 579 |
| AcsI | RAATTY | 2 cut(s) | 101, 691 |
| AcyI | GRCGYC | 1 cut(s) | 120 |
| AfaI | GTAC | 3 cut(s) | 178, 470, 557 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 320, 577, 712 |
| AgeI | ACCGGT | 1 cut(s) | 187 |
| AhlI | ACTAGT | 1 cut(s) | 68 |
| AjnI | CCWGG | 2 cut(s) | 413, 669 |
| AluBI | AGCT | 6 cut(s) | 257, 277, 292, 359, 370, 402 |
| AluI | AGCT | 6 cut(s) | 257, 277, 292, 359, 370, 402 |
| Alw21I | GWGCWC | 1 cut(s) | 48 |
| Alw26I | GTCTC | 2 cut(s) | 59, 329 |
| AlwI | GGATC | 5 cut(s) | 150, 163, 275, 416, 512 |
| AoxI | GGCC | 1 cut(s) | 579 |
| ApeKI | GCWGC | 2 cut(s) | 437, 440 |
| ApoI | RAATTY | 2 cut(s) | 101, 691 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 74, 106 |
| AsiGI | ACCGGT | 1 cut(s) | 187 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 566, 681 |
| AspLEI | GCGC | 2 cut(s) | 99, 111 |
| AsuC2I | CCSGG | 1 cut(s) | 315 |
| BalI | TGGCCA | 1 cut(s) | 581 |
| BamHI | GGATCC | 1 cut(s) | 155 |
| BanII | GRGCYC | 1 cut(s) | 493 |
| Bbv12I | GWGCWC | 1 cut(s) | 48 |
| BbvI | GCAGC | 2 cut(s) | 449, 452 |
| BccI | CCATC | 1 cut(s) | 146 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 32, 66 |
| BciT130I | CCWGG | 2 cut(s) | 415, 671 |
| BclI | TGATCA | 1 cut(s) | 10 |
| BcnI | CCSGG | 1 cut(s) | 315 |
| BcoDI | GTCTC | 2 cut(s) | 59, 329 |
| BcuI | ACTAGT | 1 cut(s) | 68 |
| BfaI | CTAG | 5 cut(s) | 69, 278, 371, 422, 698 |
| BfmI | CTRYAG | 1 cut(s) | 435 |
| BglII | AGATCT | 1 cut(s) | 429 |
| BisI | GCNGC | 2 cut(s) | 438, 441 |
| BlsI | GCNGC | 2 cut(s) | 439, 442 |
| Bme1390I | CCNGG | 3 cut(s) | 315, 415, 671 |
| BmiI | GGNNCC | 2 cut(s) | 157, 597 |
| BmrFI | CCNGG | 3 cut(s) | 315, 415, 671 |
| BmsI | GCATC | 2 cut(s) | 124, 469 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 309, 341 |
| Bpu10I | CCTNAGC | 1 cut(s) | 293 |
| BpuEI | CTTGAG | 1 cut(s) | 424 |
| BpuMI | CCSGG | 1 cut(s) | 315 |
| BsaBI | GATNNNNATC | 1 cut(s) | 138 |
| BsaHI | GRCGYC | 1 cut(s) | 120 |
| BsaI | GGTCTC | 1 cut(s) | 329 |
| BsaJI | CCNNGG | 3 cut(s) | 363, 582, 707 |
| BsaWI | WCCGGW | 1 cut(s) | 187 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 320, 577, 712 |
| Bse118I | RCCGGY | 1 cut(s) | 187 |
| Bse1I | ACTGG | 1 cut(s) | 207 |
| Bse3DI | GCAATG | 2 cut(s) | 48, 57 |
| Bse8I | GATNNNNATC | 1 cut(s) | 138 |
| BseBI | CCWGG | 2 cut(s) | 415, 671 |
| BseDI | CCNNGG | 3 cut(s) | 363, 582, 707 |
| BseGI | GGATG | 4 cut(s) | 535, 666, 679, 702 |
| BseJI | GATNNNNATC | 1 cut(s) | 138 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 320, 577, 712 |
| BseMI | GCAATG | 2 cut(s) | 48, 57 |
| BseMII | CTCAG | 2 cut(s) | 291, 307 |
| BseNI | ACTGG | 1 cut(s) | 207 |
| BseRI | GAGGAG | 1 cut(s) | 249 |
| BseXI | GCAGC | 2 cut(s) | 449, 452 |
| BshFI | GGCC | 1 cut(s) | 581 |
| BshTI | ACCGGT | 1 cut(s) | 187 |
| BsiHKAI | GWGCWC | 1 cut(s) | 48 |
| BsiSI | CCGG | 2 cut(s) | 188, 315 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 320, 577, 712 |
| BsmAI | GTCTC | 2 cut(s) | 59, 329 |
| BsnI | GGCC | 1 cut(s) | 581 |
| Bso31I | GGTCTC | 1 cut(s) | 329 |
| Bsp1286I | GDGCHC | 2 cut(s) | 48, 493 |
| Bsp143I | GATC | 7 cut(s) | 10, 155, 267, 408, 429, 504, 615 |
| Bsp19I | CCATGG | 1 cut(s) | 582 |
| BspANI | GGCC | 1 cut(s) | 581 |
| BspCNI | CTCAG | 2 cut(s) | 292, 306 |
| BspHI | TCATGA | 1 cut(s) | 493 |
| BspLI | GGNNCC | 2 cut(s) | 157, 597 |
| BspMAI | CTGCAG | 1 cut(s) | 439 |
| BspPI | GGATC | 5 cut(s) | 150, 163, 275, 416, 512 |
| BspTNI | GGTCTC | 1 cut(s) | 329 |
| BsrDI | GCAATG | 2 cut(s) | 48, 57 |
| BsrFI | RCCGGY | 1 cut(s) | 187 |
| BsrI | ACTGG | 1 cut(s) | 207 |
| BssAI | RCCGGY | 1 cut(s) | 187 |
| BssECI | CCNNGG | 3 cut(s) | 363, 582, 707 |
| BssMI | GATC | 7 cut(s) | 10, 155, 267, 408, 429, 504, 615 |
| BssNI | GRCGYC | 1 cut(s) | 120 |
| BssT1I | CCWWGG | 3 cut(s) | 363, 582, 707 |
| Bst2UI | CCWGG | 2 cut(s) | 415, 671 |
| Bst6I | CTCTTC | 2 cut(s) | 345, 528 |
| BstACI | GRCGYC | 1 cut(s) | 120 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 59 |
| BstC8I | GCNNGC | 2 cut(s) | 55, 255 |
| BstDEI | CTNAG | 3 cut(s) | 167, 293, 300 |
| BstDSI | CCRYGG | 1 cut(s) | 582 |
| BstF5I | GGATG | 4 cut(s) | 535, 666, 679, 702 |
| BstHHI | GCGC | 2 cut(s) | 99, 111 |
| BstKTI | GATC | 7 cut(s) | 13, 158, 270, 411, 432, 507, 618 |
| BstMAI | GTCTC | 2 cut(s) | 59, 329 |
| BstMBI | GATC | 7 cut(s) | 10, 155, 267, 408, 429, 504, 615 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 59, 488 |
| BstNI | CCWGG | 2 cut(s) | 415, 671 |
| BstSCI | CCNGG | 3 cut(s) | 313, 413, 669 |
| BstSFI | CTRYAG | 1 cut(s) | 435 |
| BstV1I | GCAGC | 2 cut(s) | 449, 452 |
| BstX2I | RGATCY | 2 cut(s) | 155, 429 |
| BstYI | RGATCY | 2 cut(s) | 155, 429 |
| BsuRI | GGCC | 1 cut(s) | 581 |
| BtgI | CCRYGG | 1 cut(s) | 582 |
| BtsCI | GGATG | 4 cut(s) | 535, 666, 679, 702 |
| BtsI | GCAGTG | 1 cut(s) | 327 |
| BtsIMutI | CAGTG | 1 cut(s) | 327 |
| Cac8I | GCNNGC | 2 cut(s) | 55, 255 |
| CciI | TCATGA | 1 cut(s) | 493 |
| CfoI | GCGC | 2 cut(s) | 99, 111 |
| Cfr10I | RCCGGY | 1 cut(s) | 187 |
| Csp6I | GTAC | 3 cut(s) | 177, 469, 556 |
| CspAI | ACCGGT | 1 cut(s) | 187 |
| CviAII | CATG | 3 cut(s) | 38, 494, 583 |
| CviQI | GTAC | 3 cut(s) | 177, 469, 556 |
| DdeI | CTNAG | 3 cut(s) | 167, 293, 300 |
| DpnI | GATC | 7 cut(s) | 12, 157, 269, 410, 431, 506, 617 |
| DpnII | GATC | 7 cut(s) | 10, 155, 267, 408, 429, 504, 615 |
| EaeI | YGGCCR | 1 cut(s) | 579 |
| Eam1104I | CTCTTC | 2 cut(s) | 345, 528 |
| EarI | CTCTTC | 2 cut(s) | 345, 528 |
| Eco130I | CCWWGG | 3 cut(s) | 363, 582, 707 |
| Eco24I | GRGCYC | 1 cut(s) | 493 |
| Eco31I | GGTCTC | 1 cut(s) | 329 |
| EcoRII | CCWGG | 2 cut(s) | 413, 669 |
| EcoT14I | CCWWGG | 3 cut(s) | 363, 582, 707 |
| EcoT38I | GRGCYC | 1 cut(s) | 493 |
| ErhI | CCWWGG | 3 cut(s) | 363, 582, 707 |
| FaeI | CATG | 3 cut(s) | 41, 497, 586 |
| FatI | CATG | 3 cut(s) | 37, 493, 582 |
| FbaI | TGATCA | 1 cut(s) | 10 |
| FblI | GTMKAC | 1 cut(s) | 123 |
| Fnu4HI | GCNGC | 2 cut(s) | 438, 441 |
| FokI | GGATG | 4 cut(s) | 542, 653, 686, 689 |
| FriOI | GRGCYC | 1 cut(s) | 493 |
| Fsp4HI | GCNGC | 2 cut(s) | 438, 441 |
| FspBI | CTAG | 5 cut(s) | 69, 278, 371, 422, 698 |
| FspI | TGCGCA | 2 cut(s) | 98, 110 |
| GlaI | GCGC | 2 cut(s) | 98, 110 |
| GluI | GCNGC | 2 cut(s) | 438, 441 |
| HaeIII | GGCC | 1 cut(s) | 581 |
| HapII | CCGG | 2 cut(s) | 188, 315 |
| HhaI | GCGC | 2 cut(s) | 99, 111 |
| Hin1I | GRCGYC | 1 cut(s) | 120 |
| Hin1II | CATG | 3 cut(s) | 41, 497, 586 |
| Hin6I | GCGC | 2 cut(s) | 97, 109 |
| HinP1I | GCGC | 2 cut(s) | 97, 109 |
| HincII | GTYRAC | 1 cut(s) | 124 |
| HindII | GTYRAC | 1 cut(s) | 124 |
| HinfI | GANTC | 5 cut(s) | 163, 310, 418, 562, 682 |
| HpaII | CCGG | 2 cut(s) | 188, 315 |
| Hpy166II | GTNNAC | 5 cut(s) | 124, 447, 471, 556, 589 |
| Hpy188III | TCNNGA | 3 cut(s) | 494, 502, 686 |
| Hpy8I | GTNNAC | 5 cut(s) | 124, 447, 471, 556, 589 |
| Hpy99I | CGWCG | 1 cut(s) | 125 |
| HpyAV | CCTTC | 2 cut(s) | 224, 506 |
| HpyCH4IV | ACGT | 1 cut(s) | 120 |
| HpyCH4V | TGCA | 4 cut(s) | 53, 62, 332, 437 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 59, 488 |
| HpyF3I | CTNAG | 3 cut(s) | 167, 293, 300 |
| HpySE526I | ACGT | 1 cut(s) | 120 |
| Hsp92I | GRCGYC | 1 cut(s) | 120 |
| Hsp92II | CATG | 3 cut(s) | 41, 497, 586 |
| HspAI | GCGC | 2 cut(s) | 97, 109 |
| Ksp22I | TGATCA | 1 cut(s) | 10 |
| Kzo9I | GATC | 7 cut(s) | 10, 155, 267, 408, 429, 504, 615 |
| LmnI | GCTCC | 2 cut(s) | 262, 367 |
| Lsp1109I | GCAGC | 2 cut(s) | 449, 452 |
| LweI | GCATC | 2 cut(s) | 124, 469 |
| MaeI | CTAG | 5 cut(s) | 69, 278, 371, 422, 698 |
| MaeII | ACGT | 1 cut(s) | 120 |
| MaeIII | GTNAC | 3 cut(s) | 32, 280, 520 |
| MalI | GATC | 7 cut(s) | 12, 157, 269, 410, 431, 506, 617 |
| MboI | GATC | 7 cut(s) | 10, 155, 267, 408, 429, 504, 615 |
| MboII | GAAGA | 4 cut(s) | 362, 439, 545, 550 |
| MflI | RGATCY | 2 cut(s) | 155, 429 |
| MhlI | GDGCHC | 2 cut(s) | 48, 493 |
| MlsI | TGGCCA | 1 cut(s) | 581 |
| MluCI | AATT | 4 cut(s) | 101, 245, 677, 691 |
| MluNI | TGGCCA | 1 cut(s) | 581 |
| MlyI | GAGTC | 1 cut(s) | 304 |
| MmeI | TCCRAC | 2 cut(s) | 60, 172 |
| MnlI | CCTC | 5 cut(s) | 270, 300, 346, 399, 490 |
| Mox20I | TGGCCA | 1 cut(s) | 581 |
| MroXI | GAANNNNTTC | 2 cut(s) | 566, 681 |
| MscI | TGGCCA | 1 cut(s) | 581 |
| MseI | TTAA | 2 cut(s) | 390, 733 |
| Msp20I | TGGCCA | 1 cut(s) | 581 |
| MspI | CCGG | 2 cut(s) | 188, 315 |
| MspR9I | CCNGG | 3 cut(s) | 315, 415, 671 |
| MvaI | CCWGG | 2 cut(s) | 415, 671 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 59, 488 |
| NciI | CCSGG | 1 cut(s) | 315 |
| NcoI | CCATGG | 1 cut(s) | 582 |
| NdeII | GATC | 7 cut(s) | 10, 155, 267, 408, 429, 504, 615 |
| NlaIII | CATG | 3 cut(s) | 41, 497, 586 |
| NlaIV | GGNNCC | 2 cut(s) | 157, 597 |
| NmeAIII | GCCGAG | 1 cut(s) | 67 |
| NmuCI | GTSAC | 3 cut(s) | 32, 280, 520 |
| NsbI | TGCGCA | 2 cut(s) | 98, 110 |
| PagI | TCATGA | 1 cut(s) | 493 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 129 |
| PdmI | GAANNNNTTC | 2 cut(s) | 566, 681 |
| PfeI | GAWTC | 4 cut(s) | 163, 418, 562, 682 |
| PfoI | TCCNGGA | 2 cut(s) | 313, 669 |
| PinAI | ACCGGT | 1 cut(s) | 187 |
| PkrI | GCNGC | 2 cut(s) | 439, 442 |
| PleI | GAGTC | 1 cut(s) | 304 |
| PpsI | GAGTC | 1 cut(s) | 304 |
| Psp6I | CCWGG | 2 cut(s) | 413, 669 |
| PspGI | CCWGG | 2 cut(s) | 413, 669 |
| PspN4I | GGNNCC | 2 cut(s) | 157, 597 |
| PstI | CTGCAG | 1 cut(s) | 439 |
| PsuI | RGATCY | 2 cut(s) | 155, 429 |
| RsaI | GTAC | 3 cut(s) | 178, 470, 557 |
| RsaNI | GTAC | 3 cut(s) | 177, 469, 556 |
| SalI | GTCGAC | 1 cut(s) | 122 |
| SaqAI | TTAA | 2 cut(s) | 390, 733 |
| SatI | GCNGC | 2 cut(s) | 438, 441 |
| Sau3AI | GATC | 7 cut(s) | 10, 155, 267, 408, 429, 504, 615 |
| SchI | GAGTC | 1 cut(s) | 304 |
| ScrFI | CCNGG | 3 cut(s) | 315, 415, 671 |
| SduI | GDGCHC | 2 cut(s) | 48, 493 |
| SfaNI | GCATC | 2 cut(s) | 124, 469 |
| SfcI | CTRYAG | 1 cut(s) | 435 |
| SmlI | CTYRAG | 1 cut(s) | 403 |
| SmoI | CTYRAG | 1 cut(s) | 403 |
| SpeI | ACTAGT | 1 cut(s) | 68 |
| Sse9I | AATT | 4 cut(s) | 101, 245, 677, 691 |
| SspMI | CTAG | 5 cut(s) | 69, 278, 371, 422, 698 |
| StyD4I | CCNGG | 3 cut(s) | 313, 413, 669 |
| StyI | CCWWGG | 3 cut(s) | 363, 582, 707 |
| TaiI | ACGT | 1 cut(s) | 123 |
| TaqI | TCGA | 2 cut(s) | 123, 249 |
| TasI | AATT | 4 cut(s) | 101, 245, 677, 691 |
| TfiI | GAWTC | 4 cut(s) | 163, 418, 562, 682 |
| Tru1I | TTAA | 2 cut(s) | 390, 733 |
| Tru9I | TTAA | 2 cut(s) | 390, 733 |
| TscAI | CASTG | 1 cut(s) | 334 |
| TseFI | GTSAC | 3 cut(s) | 32, 280, 520 |
| TseI | GCWGC | 2 cut(s) | 437, 440 |
| Tsp45I | GTSAC | 3 cut(s) | 32, 280, 520 |
| TspDTI | ATGAA | 4 cut(s) | 453, 525, 546, 690 |
| TspGWI | ACGGA | 1 cut(s) | 280 |
| TspRI | CASTG | 1 cut(s) | 334 |
| XapI | RAATTY | 2 cut(s) | 101, 691 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 580, 589 |
| XmiI | GTMKAC | 1 cut(s) | 123 |
| XmnI | GAANNNNTTC | 2 cut(s) | 566, 681 |
| XspI | CTAG | 5 cut(s) | 69, 278, 371, 422, 698 |
| ZraI | GACGTC | 1 cut(s) | 121 |