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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 3 cut(s) | 44, 157, 587 |
| AciI | CCGC | 6 cut(s) | 42, 139, 192, 219, 245, 387 |
| AclWI | GGATC | 3 cut(s) | 262, 505, 518 |
| AcsI | RAATTY | 2 cut(s) | 165, 581 |
| AfaI | GTAC | 1 cut(s) | 365 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 277, 336 |
| AgsI | TTSAA | 1 cut(s) | 311 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 566, 598 |
| AleI | CACNNNNGTG | 1 cut(s) | 296 |
| AluBI | AGCT | 3 cut(s) | 306, 396, 553 |
| AluI | AGCT | 3 cut(s) | 306, 396, 553 |
| Alw26I | GTCTC | 1 cut(s) | 466 |
| AlwI | GGATC | 3 cut(s) | 262, 505, 518 |
| Ama87I | CYCGRG | 3 cut(s) | 86, 195, 391 |
| AoxI | GGCC | 6 cut(s) | 38, 75, 117, 173, 388, 564 |
| ApeKI | GCWGC | 2 cut(s) | 303, 484 |
| ApoI | RAATTY | 2 cut(s) | 165, 581 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 446 |
| AspLEI | GCGC | 2 cut(s) | 159, 264 |
| AspS9I | GGNCC | 4 cut(s) | 39, 75, 118, 389 |
| AsuC2I | CCSGG | 2 cut(s) | 352, 501 |
| AsuHPI | GGTGA | 2 cut(s) | 293, 562 |
| AsuII | TTCGAA | 1 cut(s) | 169 |
| AvaI | CYCGRG | 3 cut(s) | 86, 195, 391 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 347, 380 |
| BamHI | GGATCC | 1 cut(s) | 510 |
| BanII | GRGCYC | 1 cut(s) | 87 |
| BbvI | GCAGC | 2 cut(s) | 315, 471 |
| BccI | CCATC | 2 cut(s) | 40, 556 |
| BceAI | ACGGC | 1 cut(s) | 126 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 466, 500 |
| BcnI | CCSGG | 2 cut(s) | 352, 501 |
| BcoDI | GTCTC | 1 cut(s) | 466 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 611 |
| BisI | GCNGC | 3 cut(s) | 304, 388, 485 |
| BlsI | GCNGC | 3 cut(s) | 305, 389, 486 |
| Bme1390I | CCNGG | 2 cut(s) | 352, 501 |
| BmeT110I | CYCGRG | 3 cut(s) | 86, 195, 391 |
| BmgT120I | GGNCC | 4 cut(s) | 39, 75, 118, 389 |
| BmiI | GGNNCC | 3 cut(s) | 235, 512, 543 |
| BmrFI | CCNGG | 2 cut(s) | 352, 501 |
| BmsI | GCATC | 2 cut(s) | 356, 592 |
| Bpu14I | TTCGAA | 1 cut(s) | 169 |
| BpuMI | CCSGG | 2 cut(s) | 352, 501 |
| Bsa29I | ATCGAT | 1 cut(s) | 560 |
| BsaI | GGTCTC | 1 cut(s) | 466 |
| BsaJI | CCNNGG | 4 cut(s) | 87, 142, 351, 537 |
| BsaWI | WCCGGW | 1 cut(s) | 270 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 277, 336 |
| Bse118I | RCCGGY | 1 cut(s) | 402 |
| BseCI | ATCGAT | 1 cut(s) | 560 |
| BseDI | CCNNGG | 4 cut(s) | 87, 142, 351, 537 |
| BseGI | GGATG | 3 cut(s) | 347, 459, 633 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 277, 336 |
| BseRI | GAGGAG | 2 cut(s) | 308, 484 |
| BseXI | GCAGC | 2 cut(s) | 315, 471 |
| Bsh1236I | CGCG | 3 cut(s) | 44, 157, 587 |
| Bsh1285I | CGRYCG | 1 cut(s) | 500 |
| BshFI | GGCC | 6 cut(s) | 40, 77, 119, 175, 390, 566 |
| BshVI | ATCGAT | 1 cut(s) | 560 |
| BsiEI | CGRYCG | 1 cut(s) | 500 |
| BsiHKCI | CYCGRG | 3 cut(s) | 86, 195, 391 |
| BsiSI | CCGG | 4 cut(s) | 271, 351, 403, 500 |
| BslFI | GGGAC | 2 cut(s) | 174, 369 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 277, 336 |
| BsmAI | GTCTC | 1 cut(s) | 466 |
| BsmFI | GGGAC | 2 cut(s) | 174, 369 |
| BsmI | GAATGC | 1 cut(s) | 349 |
| BsnI | GGCC | 6 cut(s) | 40, 77, 119, 175, 390, 566 |
| Bso31I | GGTCTC | 1 cut(s) | 466 |
| BsoBI | CYCGRG | 3 cut(s) | 86, 195, 391 |
| Bsp119I | TTCGAA | 1 cut(s) | 169 |
| Bsp1286I | GDGCHC | 1 cut(s) | 87 |
| Bsp143I | GATC | 2 cut(s) | 267, 510 |
| Bsp68I | TCGCGA | 1 cut(s) | 587 |
| BspACI | CCGC | 6 cut(s) | 42, 139, 192, 219, 245, 387 |
| BspANI | GGCC | 6 cut(s) | 40, 77, 119, 175, 390, 566 |
| BspDI | ATCGAT | 1 cut(s) | 560 |
| BspFNI | CGCG | 3 cut(s) | 44, 157, 587 |
| BspLI | GGNNCC | 3 cut(s) | 235, 512, 543 |
| BspPI | GGATC | 3 cut(s) | 262, 505, 518 |
| BspT104I | TTCGAA | 1 cut(s) | 169 |
| BspTNI | GGTCTC | 1 cut(s) | 466 |
| BsrFI | RCCGGY | 1 cut(s) | 402 |
| BssAI | RCCGGY | 1 cut(s) | 402 |
| BssECI | CCNNGG | 4 cut(s) | 87, 142, 351, 537 |
| BssMI | GATC | 2 cut(s) | 267, 510 |
| BssT1I | CCWWGG | 1 cut(s) | 537 |
| Bst4CI | ACNGT | 2 cut(s) | 383, 427 |
| Bst6I | CTCTTC | 1 cut(s) | 461 |
| BstBI | TTCGAA | 1 cut(s) | 169 |
| BstC8I | GCNNGC | 1 cut(s) | 42 |
| BstDSI | CCRYGG | 1 cut(s) | 142 |
| BstF5I | GGATG | 3 cut(s) | 347, 459, 633 |
| BstFNI | CGCG | 3 cut(s) | 44, 157, 587 |
| BstHHI | GCGC | 2 cut(s) | 159, 264 |
| BstKTI | GATC | 2 cut(s) | 270, 513 |
| BstMAI | GTCTC | 1 cut(s) | 466 |
| BstMBI | GATC | 2 cut(s) | 267, 510 |
| BstMCI | CGRYCG | 1 cut(s) | 500 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 251, 393, 611 |
| BstSCI | CCNGG | 2 cut(s) | 350, 499 |
| BstUI | CGCG | 3 cut(s) | 44, 157, 587 |
| BstV1I | GCAGC | 2 cut(s) | 315, 471 |
| BstX2I | RGATCY | 1 cut(s) | 510 |
| BstYI | RGATCY | 1 cut(s) | 510 |
| Bsu15I | ATCGAT | 1 cut(s) | 560 |
| BsuRI | GGCC | 6 cut(s) | 40, 77, 119, 175, 390, 566 |
| BsuTUI | ATCGAT | 1 cut(s) | 560 |
| BtgI | CCRYGG | 1 cut(s) | 142 |
| BtgZI | GCGATG | 3 cut(s) | 59, 360, 465 |
| BtsCI | GGATG | 3 cut(s) | 347, 459, 633 |
| BtuMI | TCGCGA | 1 cut(s) | 587 |
| Cac8I | GCNNGC | 1 cut(s) | 42 |
| CfoI | GCGC | 2 cut(s) | 159, 264 |
| Cfr10I | RCCGGY | 1 cut(s) | 402 |
| Cfr13I | GGNCC | 4 cut(s) | 39, 75, 118, 389 |
| ClaI | ATCGAT | 1 cut(s) | 560 |
| CseI | GACGC | 1 cut(s) | 341 |
| Csp6I | GTAC | 1 cut(s) | 364 |
| CviAII | CATG | 3 cut(s) | 258, 265, 617 |
| CviQI | GTAC | 1 cut(s) | 364 |
| DpnI | GATC | 2 cut(s) | 269, 512 |
| DpnII | GATC | 2 cut(s) | 267, 510 |
| Eam1104I | CTCTTC | 1 cut(s) | 461 |
| EarI | CTCTTC | 1 cut(s) | 461 |
| EciI | GGCGGA | 1 cut(s) | 128 |
| Eco130I | CCWWGG | 1 cut(s) | 537 |
| Eco24I | GRGCYC | 1 cut(s) | 87 |
| Eco31I | GGTCTC | 1 cut(s) | 466 |
| Eco88I | CYCGRG | 3 cut(s) | 86, 195, 391 |
| EcoRI | GAATTC | 1 cut(s) | 581 |
| EcoT14I | CCWWGG | 1 cut(s) | 537 |
| EcoT22I | ATGCAT | 1 cut(s) | 349 |
| EcoT38I | GRGCYC | 1 cut(s) | 87 |
| ErhI | CCWWGG | 1 cut(s) | 537 |
| FaeI | CATG | 3 cut(s) | 261, 268, 620 |
| FaqI | GGGAC | 2 cut(s) | 174, 369 |
| FatI | CATG | 3 cut(s) | 257, 264, 616 |
| FauI | CCCGC | 2 cut(s) | 49, 199 |
| FauNDI | CATATG | 2 cut(s) | 360, 598 |
| Fnu4HI | GCNGC | 3 cut(s) | 304, 388, 485 |
| FokI | GGATG | 2 cut(s) | 334, 466 |
| FriOI | GRGCYC | 1 cut(s) | 87 |
| Fsp4HI | GCNGC | 3 cut(s) | 304, 388, 485 |
| GlaI | GCGC | 2 cut(s) | 158, 263 |
| GluI | GCNGC | 3 cut(s) | 304, 388, 485 |
| HaeIII | GGCC | 6 cut(s) | 40, 77, 119, 175, 390, 566 |
| HapII | CCGG | 4 cut(s) | 271, 351, 403, 500 |
| HgaI | GACGC | 1 cut(s) | 341 |
| HhaI | GCGC | 2 cut(s) | 159, 264 |
| Hin1II | CATG | 3 cut(s) | 261, 268, 620 |
| Hin6I | GCGC | 2 cut(s) | 157, 262 |
| HinP1I | GCGC | 2 cut(s) | 157, 262 |
| HincII | GTYRAC | 1 cut(s) | 94 |
| HindII | GTYRAC | 1 cut(s) | 94 |
| HinfI | GANTC | 1 cut(s) | 622 |
| HpaII | CCGG | 4 cut(s) | 271, 351, 403, 500 |
| HphI | GGTGA | 2 cut(s) | 293, 562 |
| Hpy166II | GTNNAC | 2 cut(s) | 94, 284 |
| Hpy188I | TCNGA | 2 cut(s) | 62, 627 |
| Hpy188III | TCNNGA | 2 cut(s) | 451, 586 |
| Hpy8I | GTNNAC | 2 cut(s) | 94, 284 |
| Hpy99I | CGWCG | 1 cut(s) | 335 |
| HpyAV | CCTTC | 3 cut(s) | 165, 501, 577 |
| HpyCH4III | ACNGT | 2 cut(s) | 383, 427 |
| HpyCH4V | TGCA | 1 cut(s) | 347 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 251, 393, 611 |
| Hsp92II | CATG | 3 cut(s) | 261, 268, 620 |
| HspAI | GCGC | 2 cut(s) | 157, 262 |
| Kzo9I | GATC | 2 cut(s) | 267, 510 |
| LpnPI | CCDG | 5 cut(s) | 284, 364, 380, 416, 513 |
| Lsp1109I | GCAGC | 2 cut(s) | 315, 471 |
| LweI | GCATC | 2 cut(s) | 356, 592 |
| MalI | GATC | 2 cut(s) | 269, 512 |
| MboI | GATC | 2 cut(s) | 267, 510 |
| MboII | GAAGA | 5 cut(s) | 144, 260, 352, 451, 478 |
| MflI | RGATCY | 1 cut(s) | 510 |
| MhlI | GDGCHC | 1 cut(s) | 87 |
| MluCI | AATT | 2 cut(s) | 165, 581 |
| MnlI | CCTC | 6 cut(s) | 82, 247, 329, 456, 462, 497 |
| Mph1103I | ATGCAT | 1 cut(s) | 349 |
| MroXI | GAANNNNTTC | 1 cut(s) | 446 |
| MslI | CAYNNNNRTG | 3 cut(s) | 113, 296, 434 |
| MspI | CCGG | 4 cut(s) | 271, 351, 403, 500 |
| MspR9I | CCNGG | 2 cut(s) | 352, 501 |
| Mva1269I | GAATGC | 1 cut(s) | 349 |
| MvnI | CGCG | 3 cut(s) | 44, 157, 587 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 251, 393, 611 |
| NciI | CCSGG | 2 cut(s) | 352, 501 |
| NdeI | CATATG | 2 cut(s) | 360, 598 |
| NdeII | GATC | 2 cut(s) | 267, 510 |
| NlaIII | CATG | 3 cut(s) | 261, 268, 620 |
| NlaIV | GGNNCC | 3 cut(s) | 235, 512, 543 |
| NruI | TCGCGA | 1 cut(s) | 587 |
| NsiI | ATGCAT | 1 cut(s) | 349 |
| NspV | TTCGAA | 1 cut(s) | 169 |
| OliI | CACNNNNGTG | 1 cut(s) | 296 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 193, 327 |
| PctI | GAATGC | 1 cut(s) | 349 |
| PdmI | GAANNNNTTC | 1 cut(s) | 446 |
| PfeI | GAWTC | 1 cut(s) | 622 |
| PkrI | GCNGC | 3 cut(s) | 305, 389, 486 |
| PspN4I | GGNNCC | 3 cut(s) | 235, 512, 543 |
| PspPI | GGNCC | 4 cut(s) | 39, 75, 118, 389 |
| PsuI | RGATCY | 1 cut(s) | 510 |
| RruI | TCGCGA | 1 cut(s) | 587 |
| RsaI | GTAC | 1 cut(s) | 365 |
| RsaNI | GTAC | 1 cut(s) | 364 |
| RseI | CAYNNNNRTG | 3 cut(s) | 113, 296, 434 |
| SatI | GCNGC | 3 cut(s) | 304, 388, 485 |
| Sau3AI | GATC | 2 cut(s) | 267, 510 |
| Sau96I | GGNCC | 4 cut(s) | 39, 75, 118, 389 |
| ScrFI | CCNGG | 2 cut(s) | 352, 501 |
| SduI | GDGCHC | 1 cut(s) | 87 |
| SetI | ASST | 7 cut(s) | 71, 93, 239, 300, 308, 398, 555 |
| SfaNI | GCATC | 2 cut(s) | 356, 592 |
| SfuI | TTCGAA | 1 cut(s) | 169 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 113, 296, 434 |
| Sse9I | AATT | 2 cut(s) | 165, 581 |
| SsiI | CCGC | 6 cut(s) | 42, 139, 192, 219, 245, 387 |
| StyD4I | CCNGG | 2 cut(s) | 350, 499 |
| StyI | CCWWGG | 1 cut(s) | 537 |
| TaaI | ACNGT | 2 cut(s) | 383, 427 |
| TaqI | TCGA | 4 cut(s) | 17, 169, 560, 579 |
| TaqII | GACCGA | 1 cut(s) | 490 |
| TasI | AATT | 2 cut(s) | 165, 581 |
| TauI | GCSGC | 1 cut(s) | 390 |
| TfiI | GAWTC | 1 cut(s) | 622 |
| TseI | GCWGC | 2 cut(s) | 303, 484 |
| TspDTI | ATGAA | 2 cut(s) | 452, 506 |
| XapI | RAATTY | 2 cut(s) | 165, 581 |
| XmnI | GAANNNNTTC | 1 cut(s) | 446 |
| Zsp2I | ATGCAT | 1 cut(s) | 349 |