Belongs to the 'GDSL' lipolytic enzyme family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 221, 643 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 740 |
| AccII | CGCG | 3 cut(s) | 276, 434, 491 |
| AciI | CCGC | 6 cut(s) | 51, 274, 304, 432, 489, 896 |
| AclWI | GGATC | 1 cut(s) | 1022 |
| AcoI | YGGCCR | 2 cut(s) | 271, 797 |
| AcsI | RAATTY | 1 cut(s) | 703 |
| AdeI | CACNNNGTG | 1 cut(s) | 740 |
| AfaI | GTAC | 2 cut(s) | 83, 430 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 157, 303, 514, 653, 740, 795 |
| AflII | CTTAAG | 1 cut(s) | 661 |
| AflIII | ACRYGT | 1 cut(s) | 975 |
| AgsI | TTSAA | 5 cut(s) | 138, 165, 547, 703, 806 |
| AleI | CACNNNNGTG | 1 cut(s) | 903 |
| AluBI | AGCT | 2 cut(s) | 471, 750 |
| AluI | AGCT | 2 cut(s) | 471, 750 |
| Alw21I | GWGCWC | 1 cut(s) | 76 |
| Alw26I | GTCTC | 4 cut(s) | 204, 412, 613, 776 |
| AlwI | GGATC | 1 cut(s) | 1022 |
| Ama87I | CYCGRG | 1 cut(s) | 236 |
| AoxI | GGCC | 5 cut(s) | 271, 625, 655, 725, 797 |
| ApeKI | GCWGC | 1 cut(s) | 104 |
| ApoI | RAATTY | 1 cut(s) | 703 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 370, 402 |
| AspLEI | GCGC | 1 cut(s) | 836 |
| AspS9I | GGNCC | 2 cut(s) | 656, 725 |
| AsuHPI | GGTGA | 3 cut(s) | 34, 614, 954 |
| AvaI | CYCGRG | 1 cut(s) | 236 |
| AxyI | CCTNAGG | 2 cut(s) | 352, 412 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 412, 445 |
| BalI | TGGCCA | 1 cut(s) | 799 |
| BanI | GGYRCC | 2 cut(s) | 221, 643 |
| BauI | CACGAG | 1 cut(s) | 290 |
| Bbv12I | GWGCWC | 1 cut(s) | 76 |
| BbvI | GCAGC | 1 cut(s) | 116 |
| BccI | CCATC | 2 cut(s) | 122, 381 |
| BceAI | ACGGC | 2 cut(s) | 235, 286 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 503, 537 |
| BclI | TGATCA | 1 cut(s) | 9 |
| BcoDI | GTCTC | 4 cut(s) | 204, 412, 613, 776 |
| BfaI | CTAG | 3 cut(s) | 344, 648, 1039 |
| BfmI | CTRYAG | 1 cut(s) | 424 |
| BfrI | CTTAAG | 1 cut(s) | 661 |
| BisI | GCNGC | 3 cut(s) | 105, 274, 489 |
| BlpI | GCTNAGC | 1 cut(s) | 472 |
| BlsI | GCNGC | 3 cut(s) | 106, 275, 490 |
| BmeT110I | CYCGRG | 1 cut(s) | 236 |
| BmgT120I | GGNCC | 2 cut(s) | 656, 725 |
| BmiI | GGNNCC | 6 cut(s) | 223, 358, 418, 453, 645, 658 |
| BmrI | ACTGGG | 2 cut(s) | 309, 320 |
| BmuI | ACTGGG | 2 cut(s) | 309, 320 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 472 |
| BpuEI | CTTGAG | 1 cut(s) | 72 |
| BsaJI | CCNNGG | 1 cut(s) | 235 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 58, 88, 866, 896 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 157, 303, 514, 653, 740, 795 |
| Bse1I | ACTGG | 4 cut(s) | 69, 162, 304, 326 |
| Bse21I | CCTNAGG | 2 cut(s) | 352, 412 |
| Bse3DI | GCAATG | 1 cut(s) | 319 |
| BseDI | CCNNGG | 1 cut(s) | 235 |
| BseGI | GGATG | 1 cut(s) | 808 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 157, 303, 514, 653, 740, 795 |
| BseMI | GCAATG | 1 cut(s) | 319 |
| BseMII | CTCAG | 4 cut(s) | 366, 426, 486, 1005 |
| BseNI | ACTGG | 4 cut(s) | 69, 162, 304, 326 |
| BseRI | GAGGAG | 6 cut(s) | 111, 114, 117, 120, 171, 400 |
| BseX3I | CGGCCG | 1 cut(s) | 271 |
| BseXI | GCAGC | 1 cut(s) | 116 |
| BsgI | GTGCAG | 1 cut(s) | 927 |
| Bsh1236I | CGCG | 3 cut(s) | 276, 434, 491 |
| Bsh1285I | CGRYCG | 1 cut(s) | 274 |
| BshFI | GGCC | 5 cut(s) | 273, 627, 657, 727, 799 |
| BshNI | GGYRCC | 2 cut(s) | 221, 643 |
| BsiEI | CGRYCG | 1 cut(s) | 274 |
| BsiHKAI | GWGCWC | 1 cut(s) | 76 |
| BsiHKCI | CYCGRG | 1 cut(s) | 236 |
| BslFI | GGGAC | 4 cut(s) | 173, 253, 578, 692 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 157, 303, 514, 653, 740, 795 |
| BsmAI | GTCTC | 4 cut(s) | 204, 412, 613, 776 |
| BsmFI | GGGAC | 4 cut(s) | 173, 253, 578, 692 |
| BsnI | GGCC | 5 cut(s) | 273, 627, 657, 727, 799 |
| BsoBI | CYCGRG | 1 cut(s) | 236 |
| Bsp1286I | GDGCHC | 1 cut(s) | 76 |
| Bsp143I | GATC | 2 cut(s) | 9, 1027 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 472 |
| BspACI | CCGC | 6 cut(s) | 51, 274, 304, 432, 489, 896 |
| BspANI | GGCC | 5 cut(s) | 273, 627, 657, 727, 799 |
| BspCNI | CTCAG | 4 cut(s) | 365, 425, 485, 1006 |
| BspFNI | CGCG | 3 cut(s) | 276, 434, 491 |
| BspHI | TCATGA | 1 cut(s) | 688 |
| BspLI | GGNNCC | 6 cut(s) | 223, 358, 418, 453, 645, 658 |
| BspPI | GGATC | 1 cut(s) | 1022 |
| BspT107I | GGYRCC | 2 cut(s) | 221, 643 |
| BspTI | CTTAAG | 1 cut(s) | 661 |
| BsrDI | GCAATG | 1 cut(s) | 319 |
| BsrI | ACTGG | 4 cut(s) | 69, 162, 304, 326 |
| BssECI | CCNNGG | 1 cut(s) | 235 |
| BssMI | GATC | 2 cut(s) | 9, 1027 |
| BssSI | CACGAG | 1 cut(s) | 290 |
| Bst2BI | CACGAG | 1 cut(s) | 290 |
| Bst4CI | ACNGT | 6 cut(s) | 81, 365, 425, 445, 914, 966 |
| Bst6I | CTCTTC | 1 cut(s) | 203 |
| BstAFI | CTTAAG | 1 cut(s) | 661 |
| BstC8I | GCNNGC | 3 cut(s) | 250, 717, 896 |
| BstDEI | CTNAG | 6 cut(s) | 352, 403, 412, 472, 737, 1014 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 651 |
| BstF5I | GGATG | 1 cut(s) | 808 |
| BstFNI | CGCG | 3 cut(s) | 276, 434, 491 |
| BstHHI | GCGC | 1 cut(s) | 836 |
| BstKTI | GATC | 2 cut(s) | 12, 1030 |
| BstMAI | GTCTC | 4 cut(s) | 204, 412, 613, 776 |
| BstMBI | GATC | 2 cut(s) | 9, 1027 |
| BstMCI | CGRYCG | 1 cut(s) | 274 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 258 |
| BstNSI | RCATGY | 2 cut(s) | 252, 979 |
| BstSFI | CTRYAG | 1 cut(s) | 424 |
| BstUI | CGCG | 3 cut(s) | 276, 434, 491 |
| BstV1I | GCAGC | 1 cut(s) | 116 |
| BstZI | CGGCCG | 1 cut(s) | 271 |
| Bsu36I | CCTNAGG | 2 cut(s) | 352, 412 |
| BsuRI | GGCC | 5 cut(s) | 273, 627, 657, 727, 799 |
| BtgZI | GCGATG | 1 cut(s) | 20 |
| BtsCI | GGATG | 1 cut(s) | 808 |
| BtsIMutI | CAGTG | 5 cut(s) | 76, 77, 155, 297, 1011 |
| Cac8I | GCNNGC | 3 cut(s) | 250, 717, 896 |
| CciI | TCATGA | 1 cut(s) | 688 |
| CfoI | GCGC | 1 cut(s) | 836 |
| Cfr13I | GGNCC | 2 cut(s) | 656, 725 |
| CseI | GACGC | 1 cut(s) | 110 |
| Csp6I | GTAC | 2 cut(s) | 82, 429 |
| CviAII | CATG | 4 cut(s) | 249, 689, 753, 976 |
| CviQI | GTAC | 2 cut(s) | 82, 429 |
| DdeI | CTNAG | 6 cut(s) | 352, 403, 412, 472, 737, 1014 |
| DpnI | GATC | 2 cut(s) | 11, 1029 |
| DpnII | GATC | 2 cut(s) | 9, 1027 |
| DraIII | CACNNNGTG | 1 cut(s) | 740 |
| EaeI | YGGCCR | 2 cut(s) | 271, 797 |
| EagI | CGGCCG | 1 cut(s) | 271 |
| Eam1104I | CTCTTC | 1 cut(s) | 203 |
| EarI | CTCTTC | 1 cut(s) | 203 |
| EclXI | CGGCCG | 1 cut(s) | 271 |
| Eco52I | CGGCCG | 1 cut(s) | 271 |
| Eco81I | CCTNAGG | 2 cut(s) | 352, 412 |
| Eco88I | CYCGRG | 1 cut(s) | 236 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 651 |
| EcoO109I | RGGNCCY | 1 cut(s) | 656 |
| EcoRI | GAATTC | 1 cut(s) | 703 |
| FaeI | CATG | 4 cut(s) | 252, 692, 756, 979 |
| FaiI | YATR | 5 cut(s) | 250, 336, 690, 754, 977 |
| FaqI | GGGAC | 4 cut(s) | 173, 253, 578, 692 |
| FatI | CATG | 4 cut(s) | 248, 688, 752, 975 |
| FauI | CCCGC | 1 cut(s) | 903 |
| FbaI | TGATCA | 1 cut(s) | 9 |
| Fnu4HI | GCNGC | 3 cut(s) | 105, 274, 489 |
| FokI | GGATG | 1 cut(s) | 795 |
| Fsp4HI | GCNGC | 3 cut(s) | 105, 274, 489 |
| FspBI | CTAG | 3 cut(s) | 344, 648, 1039 |
| GlaI | GCGC | 1 cut(s) | 835 |
| GluI | GCNGC | 3 cut(s) | 105, 274, 489 |
| HaeIII | GGCC | 5 cut(s) | 273, 627, 657, 727, 799 |
| HgaI | GACGC | 1 cut(s) | 110 |
| HhaI | GCGC | 1 cut(s) | 836 |
| Hin1II | CATG | 4 cut(s) | 252, 692, 756, 979 |
| Hin6I | GCGC | 1 cut(s) | 834 |
| HinP1I | GCGC | 1 cut(s) | 834 |
| HincII | GTYRAC | 3 cut(s) | 862, 981, 1001 |
| HindII | GTYRAC | 3 cut(s) | 862, 981, 1001 |
| HinfI | GANTC | 7 cut(s) | 178, 212, 293, 321, 347, 503, 765 |
| HpaI | GTTAAC | 1 cut(s) | 862 |
| HphI | GGTGA | 3 cut(s) | 34, 614, 954 |
| Hpy166II | GTNNAC | 6 cut(s) | 297, 535, 856, 862, 981, 1001 |
| Hpy188I | TCNGA | 4 cut(s) | 209, 709, 928, 973 |
| Hpy188III | TCNNGA | 7 cut(s) | 89, 238, 354, 414, 689, 700, 812 |
| Hpy8I | GTNNAC | 6 cut(s) | 297, 535, 856, 862, 981, 1001 |
| Hpy99I | CGWCG | 3 cut(s) | 104, 578, 686 |
| HpyAV | CCTTC | 1 cut(s) | 144 |
| HpyCH4III | ACNGT | 6 cut(s) | 81, 365, 425, 445, 914, 966 |
| HpyCH4IV | ACGT | 2 cut(s) | 684, 858 |
| HpyCH4V | TGCA | 5 cut(s) | 36, 252, 312, 719, 908 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 258 |
| HpyF3I | CTNAG | 6 cut(s) | 352, 403, 412, 472, 737, 1014 |
| HpySE526I | ACGT | 2 cut(s) | 684, 858 |
| Hsp92II | CATG | 4 cut(s) | 252, 692, 756, 979 |
| HspAI | GCGC | 1 cut(s) | 834 |
| Ksp22I | TGATCA | 1 cut(s) | 9 |
| KspAI | GTTAAC | 1 cut(s) | 862 |
| Kzo9I | GATC | 2 cut(s) | 9, 1027 |
| LmnI | GCTCC | 3 cut(s) | 79, 356, 416 |
| Lsp1109I | GCAGC | 1 cut(s) | 116 |
| MaeI | CTAG | 3 cut(s) | 344, 648, 1039 |
| MaeII | ACGT | 2 cut(s) | 684, 858 |
| MaeIII | GTNAC | 1 cut(s) | 445 |
| MalI | GATC | 2 cut(s) | 11, 1029 |
| MboI | GATC | 2 cut(s) | 9, 1027 |
| MboII | GAAGA | 3 cut(s) | 8, 11, 190 |
| MhlI | GDGCHC | 1 cut(s) | 76 |
| MlsI | TGGCCA | 1 cut(s) | 799 |
| MluCI | AATT | 3 cut(s) | 229, 703, 886 |
| MluNI | TGGCCA | 1 cut(s) | 799 |
| MlyI | GAGTC | 3 cut(s) | 172, 206, 302 |
| Mox20I | TGGCCA | 1 cut(s) | 799 |
| MscI | TGGCCA | 1 cut(s) | 799 |
| MseI | TTAA | 4 cut(s) | 618, 662, 668, 861 |
| MslI | CAYNNNNRTG | 2 cut(s) | 247, 903 |
| Msp20I | TGGCCA | 1 cut(s) | 799 |
| MspCI | CTTAAG | 1 cut(s) | 661 |
| MvnI | CGCG | 3 cut(s) | 276, 434, 491 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 258 |
| NdeII | GATC | 2 cut(s) | 9, 1027 |
| NlaIII | CATG | 4 cut(s) | 252, 692, 756, 979 |
| NlaIV | GGNNCC | 6 cut(s) | 223, 358, 418, 453, 645, 658 |
| NspI | RCATGY | 2 cut(s) | 252, 979 |
| OliI | CACNNNNGTG | 1 cut(s) | 903 |
| PaeI | GCATGC | 1 cut(s) | 252 |
| PagI | TCATGA | 1 cut(s) | 688 |
| PciI | ACATGT | 1 cut(s) | 975 |
| PfeI | GAWTC | 4 cut(s) | 321, 347, 503, 765 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 740 |
| PkrI | GCNGC | 3 cut(s) | 106, 275, 490 |
| PleI | GAGTC | 3 cut(s) | 172, 206, 301 |
| PpsI | GAGTC | 3 cut(s) | 172, 206, 301 |
| PscI | ACATGT | 1 cut(s) | 975 |
| PspN4I | GGNNCC | 6 cut(s) | 223, 358, 418, 453, 645, 658 |
| PspPI | GGNCC | 2 cut(s) | 656, 725 |
| RsaI | GTAC | 2 cut(s) | 83, 430 |
| RsaNI | GTAC | 2 cut(s) | 82, 429 |
| RseI | CAYNNNNRTG | 2 cut(s) | 247, 903 |
| SaqAI | TTAA | 4 cut(s) | 618, 662, 668, 861 |
| SatI | GCNGC | 3 cut(s) | 105, 274, 489 |
| Sau3AI | GATC | 2 cut(s) | 9, 1027 |
| Sau96I | GGNCC | 2 cut(s) | 656, 725 |
| SchI | GAGTC | 3 cut(s) | 172, 206, 302 |
| SduI | GDGCHC | 1 cut(s) | 76 |
| SfcI | CTRYAG | 1 cut(s) | 424 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 247, 903 |
| SmlI | CTYRAG | 2 cut(s) | 87, 661 |
| SmoI | CTYRAG | 2 cut(s) | 87, 661 |
| SphI | GCATGC | 1 cut(s) | 252 |
| Sse9I | AATT | 3 cut(s) | 229, 703, 886 |
| SsiI | CCGC | 6 cut(s) | 51, 274, 304, 432, 489, 896 |
| SspI | AATATT | 1 cut(s) | 622 |
| SspMI | CTAG | 3 cut(s) | 344, 648, 1039 |
| TaaI | ACNGT | 6 cut(s) | 81, 365, 425, 445, 914, 966 |
| TaiI | ACGT | 2 cut(s) | 687, 861 |
| TaqI | TCGA | 2 cut(s) | 99, 330 |
| TaqII | GACCGA | 1 cut(s) | 388 |
| TasI | AATT | 3 cut(s) | 229, 703, 886 |
| TatI | WGTACW | 1 cut(s) | 81 |
| TauI | GCSGC | 2 cut(s) | 276, 491 |
| TfiI | GAWTC | 4 cut(s) | 321, 347, 503, 765 |
| Tru1I | TTAA | 4 cut(s) | 618, 662, 668, 861 |
| Tru9I | TTAA | 4 cut(s) | 618, 662, 668, 861 |
| TscAI | CASTG | 5 cut(s) | 76, 84, 162, 304, 1018 |
| TseI | GCWGC | 1 cut(s) | 104 |
| TspDTI | ATGAA | 1 cut(s) | 677 |
| TspGWI | ACGGA | 2 cut(s) | 464, 609 |
| TspRI | CASTG | 5 cut(s) | 76, 84, 162, 304, 1018 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 740 |
| Vha464I | CTTAAG | 1 cut(s) | 661 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 651 |
| XapI | RAATTY | 1 cut(s) | 703 |
| XceI | RCATGY | 2 cut(s) | 252, 979 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 515 |
| XspI | CTAG | 3 cut(s) | 344, 648, 1039 |