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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 397 |
| AccB1I | GGYRCC | 1 cut(s) | 50 |
| AccII | CGCG | 1 cut(s) | 285 |
| AciI | CCGC | 6 cut(s) | 14, 324, 327, 330, 411, 440 |
| AclWI | GGATC | 1 cut(s) | 209 |
| AcoI | YGGCCR | 2 cut(s) | 158, 241 |
| AcyI | GRCGYC | 1 cut(s) | 51 |
| AfaI | GTAC | 1 cut(s) | 266 |
| AgsI | TTSAA | 3 cut(s) | 58, 179, 343 |
| AluBI | AGCT | 2 cut(s) | 74, 446 |
| AluI | AGCT | 2 cut(s) | 74, 446 |
| AlwI | GGATC | 1 cut(s) | 209 |
| AoxI | GGCC | 6 cut(s) | 136, 158, 205, 241, 291, 357 |
| ApeKI | GCWGC | 3 cut(s) | 80, 83, 446 |
| AspLEI | GCGC | 3 cut(s) | 53, 285, 377 |
| AspS9I | GGNCC | 1 cut(s) | 136 |
| BalI | TGGCCA | 2 cut(s) | 160, 243 |
| BanI | GGYRCC | 1 cut(s) | 50 |
| BbvI | GCAGC | 3 cut(s) | 67, 70, 433 |
| BccI | CCATC | 2 cut(s) | 126, 256 |
| BceAI | ACGGC | 1 cut(s) | 53 |
| BfoI | RGCGCY | 1 cut(s) | 54 |
| BfuAI | ACCTGC | 1 cut(s) | 397 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 59, 144 |
| BglII | AGATCT | 1 cut(s) | 119 |
| BisI | GCNGC | 6 cut(s) | 81, 84, 327, 330, 411, 447 |
| BlsI | GCNGC | 6 cut(s) | 82, 85, 328, 331, 412, 448 |
| BmgT120I | GGNCC | 1 cut(s) | 136 |
| BmiI | GGNNCC | 2 cut(s) | 52, 137 |
| BmsI | GCATC | 2 cut(s) | 114, 187 |
| BsaAI | YACGTR | 1 cut(s) | 268 |
| BsaHI | GRCGYC | 1 cut(s) | 51 |
| BsaJI | CCNNGG | 2 cut(s) | 208, 229 |
| BsaWI | WCCGGW | 1 cut(s) | 251 |
| BseDI | CCNNGG | 2 cut(s) | 208, 229 |
| BseGI | GGATG | 2 cut(s) | 137, 248 |
| BseXI | GCAGC | 3 cut(s) | 67, 70, 433 |
| BsgI | GTGCAG | 1 cut(s) | 69 |
| Bsh1236I | CGCG | 1 cut(s) | 285 |
| BshFI | GGCC | 6 cut(s) | 138, 160, 207, 243, 293, 359 |
| BshNI | GGYRCC | 1 cut(s) | 50 |
| BsiSI | CCGG | 1 cut(s) | 252 |
| BsnI | GGCC | 6 cut(s) | 138, 160, 207, 243, 293, 359 |
| Bsp143I | GATC | 2 cut(s) | 119, 214 |
| BspACI | CCGC | 6 cut(s) | 14, 324, 327, 330, 411, 440 |
| BspANI | GGCC | 6 cut(s) | 138, 160, 207, 243, 293, 359 |
| BspFNI | CGCG | 1 cut(s) | 285 |
| BspLI | GGNNCC | 2 cut(s) | 52, 137 |
| BspMI | ACCTGC | 1 cut(s) | 397 |
| BspPI | GGATC | 1 cut(s) | 209 |
| BspT107I | GGYRCC | 1 cut(s) | 50 |
| BssECI | CCNNGG | 2 cut(s) | 208, 229 |
| BssMI | GATC | 2 cut(s) | 119, 214 |
| BssNI | GRCGYC | 1 cut(s) | 51 |
| BssT1I | CCWWGG | 2 cut(s) | 208, 229 |
| Bst4CI | ACNGT | 2 cut(s) | 24, 32 |
| BstACI | GRCGYC | 1 cut(s) | 51 |
| BstBAI | YACGTR | 1 cut(s) | 268 |
| BstC8I | GCNNGC | 2 cut(s) | 245, 357 |
| BstF5I | GGATG | 2 cut(s) | 137, 248 |
| BstFNI | CGCG | 1 cut(s) | 285 |
| BstH2I | RGCGCY | 1 cut(s) | 54 |
| BstHHI | GCGC | 3 cut(s) | 53, 285, 377 |
| BstKTI | GATC | 2 cut(s) | 122, 217 |
| BstMBI | GATC | 2 cut(s) | 119, 214 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 59, 80, 144, 240, 446 |
| BstNSI | RCATGY | 1 cut(s) | 410 |
| BstUI | CGCG | 1 cut(s) | 285 |
| BstV1I | GCAGC | 3 cut(s) | 67, 70, 433 |
| BstX2I | RGATCY | 1 cut(s) | 119 |
| BstYI | RGATCY | 1 cut(s) | 119 |
| BsuRI | GGCC | 6 cut(s) | 138, 160, 207, 243, 293, 359 |
| BtsCI | GGATG | 2 cut(s) | 137, 248 |
| BtsIMutI | CAGTG | 1 cut(s) | 37 |
| BveI | ACCTGC | 1 cut(s) | 397 |
| Cac8I | GCNNGC | 2 cut(s) | 245, 357 |
| CfoI | GCGC | 3 cut(s) | 53, 285, 377 |
| Cfr13I | GGNCC | 1 cut(s) | 136 |
| CseI | GACGC | 2 cut(s) | 26, 438 |
| Csp6I | GTAC | 1 cut(s) | 265 |
| CviAII | CATG | 3 cut(s) | 162, 183, 407 |
| CviQI | GTAC | 1 cut(s) | 265 |
| DinI | GGCGCC | 1 cut(s) | 52 |
| DpnI | GATC | 2 cut(s) | 121, 216 |
| DpnII | GATC | 2 cut(s) | 119, 214 |
| EaeI | YGGCCR | 2 cut(s) | 158, 241 |
| Eco130I | CCWWGG | 2 cut(s) | 208, 229 |
| Eco147I | AGGCCT | 3 cut(s) | 207, 293, 359 |
| EcoT14I | CCWWGG | 2 cut(s) | 208, 229 |
| EgeI | GGCGCC | 1 cut(s) | 52 |
| EheI | GGCGCC | 1 cut(s) | 52 |
| ErhI | CCWWGG | 2 cut(s) | 208, 229 |
| FaeI | CATG | 3 cut(s) | 165, 186, 410 |
| FaiI | YATR | 5 cut(s) | 12, 114, 163, 184, 408 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 376, 408 |
| FatI | CATG | 3 cut(s) | 161, 182, 406 |
| FauI | CCCGC | 1 cut(s) | 433 |
| Fnu4HI | GCNGC | 6 cut(s) | 81, 84, 327, 330, 411, 447 |
| FokI | GGATG | 2 cut(s) | 144, 235 |
| Fsp4HI | GCNGC | 6 cut(s) | 81, 84, 327, 330, 411, 447 |
| GlaI | GCGC | 3 cut(s) | 52, 284, 376 |
| GluI | GCNGC | 6 cut(s) | 81, 84, 327, 330, 411, 447 |
| HaeII | RGCGCY | 1 cut(s) | 54 |
| HaeIII | GGCC | 6 cut(s) | 138, 160, 207, 243, 293, 359 |
| HapII | CCGG | 1 cut(s) | 252 |
| HgaI | GACGC | 2 cut(s) | 26, 438 |
| HhaI | GCGC | 3 cut(s) | 53, 285, 377 |
| Hin1I | GRCGYC | 1 cut(s) | 51 |
| Hin1II | CATG | 3 cut(s) | 165, 186, 410 |
| Hin6I | GCGC | 3 cut(s) | 51, 283, 375 |
| HinP1I | GCGC | 3 cut(s) | 51, 283, 375 |
| HinfI | GANTC | 3 cut(s) | 4, 179, 277 |
| HpaII | CCGG | 1 cut(s) | 252 |
| Hpy166II | GTNNAC | 1 cut(s) | 404 |
| Hpy8I | GTNNAC | 1 cut(s) | 404 |
| Hpy99I | CGWCG | 3 cut(s) | 308, 319, 454 |
| HpyAV | CCTTC | 3 cut(s) | 52, 182, 427 |
| HpyCH4III | ACNGT | 2 cut(s) | 24, 32 |
| HpyCH4IV | ACGT | 2 cut(s) | 267, 303 |
| HpyCH4V | TGCA | 3 cut(s) | 27, 86, 200 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 59, 80, 144, 240, 446 |
| HpySE526I | ACGT | 2 cut(s) | 267, 303 |
| Hsp92I | GRCGYC | 1 cut(s) | 51 |
| Hsp92II | CATG | 3 cut(s) | 165, 186, 410 |
| HspAI | GCGC | 3 cut(s) | 51, 283, 375 |
| KasI | GGCGCC | 1 cut(s) | 50 |
| Kzo9I | GATC | 2 cut(s) | 119, 214 |
| LmnI | GCTCC | 2 cut(s) | 352, 443 |
| LpnPI | CCDG | 4 cut(s) | 257, 265, 341, 402 |
| Lsp1109I | GCAGC | 3 cut(s) | 67, 70, 433 |
| LweI | GCATC | 2 cut(s) | 114, 187 |
| MaeII | ACGT | 2 cut(s) | 267, 303 |
| MaeIII | GTNAC | 1 cut(s) | 34 |
| MalI | GATC | 2 cut(s) | 121, 216 |
| MboI | GATC | 2 cut(s) | 119, 214 |
| MboII | GAAGA | 3 cut(s) | 114, 303, 355 |
| MflI | RGATCY | 1 cut(s) | 119 |
| MlsI | TGGCCA | 2 cut(s) | 160, 243 |
| MluCI | AATT | 1 cut(s) | 174 |
| MluNI | TGGCCA | 2 cut(s) | 160, 243 |
| Mly113I | GGCGCC | 1 cut(s) | 51 |
| MnlI | CCTC | 2 cut(s) | 40, 439 |
| Mox20I | TGGCCA | 2 cut(s) | 160, 243 |
| MscI | TGGCCA | 2 cut(s) | 160, 243 |
| Msp20I | TGGCCA | 2 cut(s) | 160, 243 |
| MspI | CCGG | 1 cut(s) | 252 |
| MvnI | CGCG | 1 cut(s) | 285 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 59, 80, 144, 240, 446 |
| NarI | GGCGCC | 1 cut(s) | 51 |
| NdeII | GATC | 2 cut(s) | 119, 214 |
| NlaIII | CATG | 3 cut(s) | 165, 186, 410 |
| NlaIV | GGNNCC | 2 cut(s) | 52, 137 |
| NmeAIII | GCCGAG | 1 cut(s) | 351 |
| NmuCI | GTSAC | 1 cut(s) | 34 |
| NspI | RCATGY | 1 cut(s) | 410 |
| PceI | AGGCCT | 3 cut(s) | 207, 293, 359 |
| PfeI | GAWTC | 2 cut(s) | 179, 277 |
| PkrI | GCNGC | 6 cut(s) | 82, 85, 328, 331, 412, 448 |
| PluTI | GGCGCC | 1 cut(s) | 54 |
| Ppu21I | YACGTR | 1 cut(s) | 268 |
| PspN4I | GGNNCC | 2 cut(s) | 52, 137 |
| PspPI | GGNCC | 1 cut(s) | 136 |
| PsuI | RGATCY | 1 cut(s) | 119 |
| RsaI | GTAC | 1 cut(s) | 266 |
| RsaNI | GTAC | 1 cut(s) | 265 |
| SatI | GCNGC | 6 cut(s) | 81, 84, 327, 330, 411, 447 |
| Sau3AI | GATC | 2 cut(s) | 119, 214 |
| Sau96I | GGNCC | 1 cut(s) | 136 |
| SetI | ASST | 5 cut(s) | 76, 270, 306, 391, 448 |
| SfaNI | GCATC | 2 cut(s) | 114, 187 |
| SfoI | GGCGCC | 1 cut(s) | 52 |
| Sse9I | AATT | 1 cut(s) | 174 |
| SseBI | AGGCCT | 3 cut(s) | 207, 293, 359 |
| SsiI | CCGC | 6 cut(s) | 14, 324, 327, 330, 411, 440 |
| SspDI | GGCGCC | 1 cut(s) | 50 |
| StuI | AGGCCT | 3 cut(s) | 207, 293, 359 |
| StyI | CCWWGG | 2 cut(s) | 208, 229 |
| TaaI | ACNGT | 2 cut(s) | 24, 32 |
| TaiI | ACGT | 2 cut(s) | 270, 306 |
| TaqI | TCGA | 3 cut(s) | 152, 317, 431 |
| TasI | AATT | 1 cut(s) | 174 |
| TauI | GCSGC | 3 cut(s) | 329, 332, 413 |
| TfiI | GAWTC | 2 cut(s) | 179, 277 |
| TscAI | CASTG | 1 cut(s) | 37 |
| TseFI | GTSAC | 1 cut(s) | 34 |
| TseI | GCWGC | 3 cut(s) | 80, 83, 446 |
| Tsp45I | GTSAC | 1 cut(s) | 34 |
| TspDTI | ATGAA | 1 cut(s) | 384 |
| TspRI | CASTG | 1 cut(s) | 37 |
| XceI | RCATGY | 1 cut(s) | 410 |