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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 233 |
| AccBSI | CCGCTC | 1 cut(s) | 95 |
| AccII | CGCG | 1 cut(s) | 338 |
| AciI | CCGC | 4 cut(s) | 93, 217, 433, 517 |
| AclWI | GGATC | 2 cut(s) | 116, 218 |
| AcoI | YGGCCR | 1 cut(s) | 492 |
| AcsI | RAATTY | 1 cut(s) | 463 |
| AcyI | GRCGYC | 1 cut(s) | 354 |
| AfeI | AGCGCT | 1 cut(s) | 275 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 233 |
| AgsI | TTSAA | 1 cut(s) | 280 |
| AluBI | AGCT | 3 cut(s) | 10, 32, 242 |
| AluI | AGCT | 3 cut(s) | 10, 32, 242 |
| Alw26I | GTCTC | 1 cut(s) | 14 |
| AlwI | GGATC | 2 cut(s) | 116, 218 |
| Ama87I | CYCGRG | 2 cut(s) | 41, 152 |
| Aor51HI | AGCGCT | 1 cut(s) | 275 |
| AoxI | GGCC | 2 cut(s) | 72, 492 |
| ApoI | RAATTY | 1 cut(s) | 463 |
| AspLEI | GCGC | 1 cut(s) | 276 |
| AspS9I | GGNCC | 3 cut(s) | 73, 419, 514 |
| AsuHPI | GGTGA | 2 cut(s) | 256, 415 |
| AvaI | CYCGRG | 2 cut(s) | 41, 152 |
| AvaII | GGWCC | 2 cut(s) | 419, 514 |
| BanII | GRGCYC | 2 cut(s) | 42, 297 |
| BauI | CACGAG | 1 cut(s) | 475 |
| BbsI | GAAGAC | 1 cut(s) | 413 |
| BccI | CCATC | 2 cut(s) | 64, 394 |
| BceAI | ACGGC | 2 cut(s) | 377, 507 |
| BcoDI | GTCTC | 1 cut(s) | 14 |
| BfaI | CTAG | 2 cut(s) | 119, 377 |
| BfoI | RGCGCY | 1 cut(s) | 277 |
| Bme18I | GGWCC | 2 cut(s) | 419, 514 |
| BmeT110I | CYCGRG | 2 cut(s) | 41, 152 |
| BmgT120I | GGNCC | 3 cut(s) | 73, 419, 514 |
| BmiI | GGNNCC | 3 cut(s) | 192, 294, 421 |
| BmsI | GCATC | 1 cut(s) | 444 |
| BpiI | GAAGAC | 1 cut(s) | 413 |
| BpuEI | CTTGAG | 1 cut(s) | 350 |
| BsaHI | GRCGYC | 1 cut(s) | 354 |
| BsaJI | CCNNGG | 2 cut(s) | 42, 288 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 233 |
| BseDI | CCNNGG | 2 cut(s) | 42, 288 |
| BseGI | GGATG | 1 cut(s) | 75 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 233 |
| BseX3I | CGGCCG | 1 cut(s) | 492 |
| Bsh1236I | CGCG | 1 cut(s) | 338 |
| Bsh1285I | CGRYCG | 1 cut(s) | 495 |
| BshFI | GGCC | 2 cut(s) | 74, 494 |
| BsiEI | CGRYCG | 1 cut(s) | 495 |
| BsiHKCI | CYCGRG | 2 cut(s) | 41, 152 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 233 |
| BsmAI | GTCTC | 1 cut(s) | 14 |
| BsnI | GGCC | 2 cut(s) | 74, 494 |
| BsoBI | CYCGRG | 2 cut(s) | 41, 152 |
| Bsp1286I | GDGCHC | 2 cut(s) | 42, 297 |
| Bsp143I | GATC | 2 cut(s) | 121, 223 |
| Bsp68I | TCGCGA | 1 cut(s) | 338 |
| BspACI | CCGC | 4 cut(s) | 93, 217, 433, 517 |
| BspANI | GGCC | 2 cut(s) | 74, 494 |
| BspFNI | CGCG | 1 cut(s) | 338 |
| BspLI | GGNNCC | 3 cut(s) | 192, 294, 421 |
| BspPI | GGATC | 2 cut(s) | 116, 218 |
| BspQI | GCTCTTC | 1 cut(s) | 17 |
| BsrBI | CCGCTC | 1 cut(s) | 95 |
| BssECI | CCNNGG | 2 cut(s) | 42, 288 |
| BssMI | GATC | 2 cut(s) | 121, 223 |
| BssNI | GRCGYC | 1 cut(s) | 354 |
| BssSI | CACGAG | 1 cut(s) | 475 |
| BssT1I | CCWWGG | 1 cut(s) | 288 |
| Bst2BI | CACGAG | 1 cut(s) | 475 |
| Bst6I | CTCTTC | 1 cut(s) | 17 |
| BstACI | GRCGYC | 1 cut(s) | 354 |
| BstF5I | GGATG | 1 cut(s) | 75 |
| BstFNI | CGCG | 1 cut(s) | 338 |
| BstH2I | RGCGCY | 1 cut(s) | 277 |
| BstHHI | GCGC | 1 cut(s) | 276 |
| BstKTI | GATC | 2 cut(s) | 124, 226 |
| BstMAI | GTCTC | 1 cut(s) | 14 |
| BstMBI | GATC | 2 cut(s) | 121, 223 |
| BstMCI | CGRYCG | 1 cut(s) | 495 |
| BstUI | CGCG | 1 cut(s) | 338 |
| BstV2I | GAAGAC | 1 cut(s) | 413 |
| BstX2I | RGATCY | 1 cut(s) | 121 |
| BstYI | RGATCY | 1 cut(s) | 121 |
| BstZI | CGGCCG | 1 cut(s) | 492 |
| BsuRI | GGCC | 2 cut(s) | 74, 494 |
| BtsCI | GGATG | 1 cut(s) | 75 |
| BtuMI | TCGCGA | 1 cut(s) | 338 |
| CfoI | GCGC | 1 cut(s) | 276 |
| Cfr13I | GGNCC | 3 cut(s) | 73, 419, 514 |
| CseI | GACGC | 1 cut(s) | 362 |
| CviAII | CATG | 1 cut(s) | 213 |
| DpnI | GATC | 2 cut(s) | 123, 225 |
| DpnII | GATC | 2 cut(s) | 121, 223 |
| EaeI | YGGCCR | 1 cut(s) | 492 |
| EagI | CGGCCG | 1 cut(s) | 492 |
| Eam1104I | CTCTTC | 1 cut(s) | 17 |
| EarI | CTCTTC | 1 cut(s) | 17 |
| EclXI | CGGCCG | 1 cut(s) | 492 |
| Eco130I | CCWWGG | 1 cut(s) | 288 |
| Eco24I | GRGCYC | 2 cut(s) | 42, 297 |
| Eco47I | GGWCC | 2 cut(s) | 419, 514 |
| Eco47III | AGCGCT | 1 cut(s) | 275 |
| Eco52I | CGGCCG | 1 cut(s) | 492 |
| Eco88I | CYCGRG | 2 cut(s) | 41, 152 |
| EcoT14I | CCWWGG | 1 cut(s) | 288 |
| EcoT38I | GRGCYC | 2 cut(s) | 42, 297 |
| ErhI | CCWWGG | 1 cut(s) | 288 |
| FaeI | CATG | 1 cut(s) | 216 |
| FaiI | YATR | 9 cut(s) | 104, 149, 214, 222, 343, 345, 349, 351, 369 |
| FatI | CATG | 1 cut(s) | 212 |
| FauNDI | CATATG | 1 cut(s) | 349 |
| FokI | GGATG | 1 cut(s) | 82 |
| FriOI | GRGCYC | 2 cut(s) | 42, 297 |
| FspBI | CTAG | 2 cut(s) | 119, 377 |
| GlaI | GCGC | 1 cut(s) | 275 |
| HaeII | RGCGCY | 1 cut(s) | 277 |
| HaeIII | GGCC | 2 cut(s) | 74, 494 |
| HgaI | GACGC | 1 cut(s) | 362 |
| HhaI | GCGC | 1 cut(s) | 276 |
| Hin1I | GRCGYC | 1 cut(s) | 354 |
| Hin1II | CATG | 1 cut(s) | 216 |
| Hin6I | GCGC | 1 cut(s) | 274 |
| HinP1I | GCGC | 1 cut(s) | 274 |
| HincII | GTYRAC | 1 cut(s) | 49 |
| HindII | GTYRAC | 1 cut(s) | 49 |
| HinfI | GANTC | 3 cut(s) | 89, 373, 481 |
| HphI | GGTGA | 2 cut(s) | 256, 415 |
| Hpy166II | GTNNAC | 1 cut(s) | 49 |
| Hpy188I | TCNGA | 4 cut(s) | 17, 69, 126, 419 |
| Hpy188III | TCNNGA | 4 cut(s) | 119, 248, 337, 377 |
| Hpy8I | GTNNAC | 1 cut(s) | 49 |
| Hpy99I | CGWCG | 1 cut(s) | 102 |
| HpyAV | CCTTC | 2 cut(s) | 328, 433 |
| Hsp92I | GRCGYC | 1 cut(s) | 354 |
| Hsp92II | CATG | 1 cut(s) | 216 |
| HspAI | GCGC | 1 cut(s) | 274 |
| Kzo9I | GATC | 2 cut(s) | 121, 223 |
| LguI | GCTCTTC | 1 cut(s) | 17 |
| LmnI | GCTCC | 1 cut(s) | 292 |
| LweI | GCATC | 1 cut(s) | 444 |
| MaeI | CTAG | 2 cut(s) | 119, 377 |
| MalI | GATC | 2 cut(s) | 123, 225 |
| MbiI | CCGCTC | 1 cut(s) | 95 |
| MboI | GATC | 2 cut(s) | 121, 223 |
| MboII | GAAGA | 5 cut(s) | 4, 215, 269, 409, 418 |
| MflI | RGATCY | 1 cut(s) | 121 |
| MhlI | GDGCHC | 2 cut(s) | 42, 297 |
| MluCI | AATT | 2 cut(s) | 161, 463 |
| MlyI | GAGTC | 1 cut(s) | 98 |
| MmeI | TCCRAC | 1 cut(s) | 251 |
| MnlI | CCTC | 3 cut(s) | 37, 437, 484 |
| MseI | TTAA | 2 cut(s) | 269, 509 |
| MvnI | CGCG | 1 cut(s) | 338 |
| NdeI | CATATG | 1 cut(s) | 349 |
| NdeII | GATC | 2 cut(s) | 121, 223 |
| NlaIII | CATG | 1 cut(s) | 216 |
| NlaIV | GGNNCC | 3 cut(s) | 192, 294, 421 |
| NmeAIII | GCCGAG | 1 cut(s) | 162 |
| NruI | TCGCGA | 1 cut(s) | 338 |
| PciSI | GCTCTTC | 1 cut(s) | 17 |
| PfeI | GAWTC | 2 cut(s) | 373, 481 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 233 |
| PleI | GAGTC | 1 cut(s) | 97 |
| PpsI | GAGTC | 1 cut(s) | 97 |
| PspN4I | GGNNCC | 3 cut(s) | 192, 294, 421 |
| PspPI | GGNCC | 3 cut(s) | 73, 419, 514 |
| PsuI | RGATCY | 1 cut(s) | 121 |
| RruI | TCGCGA | 1 cut(s) | 338 |
| SapI | GCTCTTC | 1 cut(s) | 17 |
| SaqAI | TTAA | 2 cut(s) | 269, 509 |
| Sau3AI | GATC | 2 cut(s) | 121, 223 |
| Sau96I | GGNCC | 3 cut(s) | 73, 419, 514 |
| SchI | GAGTC | 1 cut(s) | 98 |
| SduI | GDGCHC | 2 cut(s) | 42, 297 |
| SetI | ASST | 6 cut(s) | 12, 34, 48, 244, 269, 476 |
| SfaNI | GCATC | 1 cut(s) | 444 |
| SinI | GGWCC | 2 cut(s) | 419, 514 |
| SmlI | CTYRAG | 1 cut(s) | 329 |
| SmoI | CTYRAG | 1 cut(s) | 329 |
| Sse9I | AATT | 2 cut(s) | 161, 463 |
| SsiI | CCGC | 4 cut(s) | 93, 217, 433, 517 |
| SspMI | CTAG | 2 cut(s) | 119, 377 |
| StyI | CCWWGG | 1 cut(s) | 288 |
| TasI | AATT | 2 cut(s) | 161, 463 |
| TfiI | GAWTC | 2 cut(s) | 373, 481 |
| Tru1I | TTAA | 2 cut(s) | 269, 509 |
| Tru9I | TTAA | 2 cut(s) | 269, 509 |
| TspDTI | ATGAA | 1 cut(s) | 396 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 233 |
| VpaK11BI | GGWCC | 2 cut(s) | 419, 514 |
| XapI | RAATTY | 1 cut(s) | 463 |
| XbaI | TCTAGA | 2 cut(s) | 118, 376 |
| XspI | CTAG | 2 cut(s) | 119, 377 |