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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 163 |
| AccII | CGCG | 4 cut(s) | 84, 194, 464, 521 |
| AccIII | TCCGGA | 2 cut(s) | 205, 388 |
| AciI | CCGC | 7 cut(s) | 4, 72, 176, 231, 258, 300, 519 |
| AclWI | GGATC | 1 cut(s) | 15 |
| AcyI | GRCGYC | 1 cut(s) | 164 |
| AdeI | CACNNNGTG | 1 cut(s) | 336 |
| AfaI | GTAC | 2 cut(s) | 17, 404 |
| AgsI | TTSAA | 1 cut(s) | 201 |
| AjiI | CACGTC | 1 cut(s) | 279 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 443, 475 |
| AluBI | AGCT | 4 cut(s) | 53, 254, 344, 429 |
| AluI | AGCT | 4 cut(s) | 53, 254, 344, 429 |
| AlwI | GGATC | 1 cut(s) | 15 |
| Ama87I | CYCGRG | 3 cut(s) | 126, 234, 455 |
| Aor13HI | TCCGGA | 2 cut(s) | 205, 388 |
| AoxI | GGCC | 3 cut(s) | 157, 182, 441 |
| ApeKI | GCWGC | 2 cut(s) | 31, 341 |
| AspLEI | GCGC | 2 cut(s) | 166, 493 |
| AspS9I | GGNCC | 2 cut(s) | 158, 545 |
| AsuC2I | CCSGG | 2 cut(s) | 152, 161 |
| AsuHPI | GGTGA | 2 cut(s) | 140, 541 |
| AsuII | TTCGAA | 1 cut(s) | 347 |
| AsuNHI | GCTAGC | 1 cut(s) | 254 |
| AvaI | CYCGRG | 3 cut(s) | 126, 234, 455 |
| AvaII | GGWCC | 1 cut(s) | 545 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 386, 419 |
| BanI | GGYRCC | 1 cut(s) | 163 |
| BanII | GRGCYC | 1 cut(s) | 127 |
| BauI | CACGAG | 1 cut(s) | 331 |
| BbvI | GCAGC | 2 cut(s) | 18, 353 |
| BccI | CCATC | 4 cut(s) | 34, 80, 149, 520 |
| BceAI | ACGGC | 1 cut(s) | 163 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 74, 108 |
| BcnI | CCSGG | 2 cut(s) | 152, 161 |
| BfaI | CTAG | 2 cut(s) | 255, 503 |
| BfoI | RGCGCY | 1 cut(s) | 167 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 488 |
| BisI | GCNGC | 4 cut(s) | 32, 301, 342, 519 |
| BlsI | GCNGC | 4 cut(s) | 33, 302, 343, 520 |
| Bme1390I | CCNGG | 2 cut(s) | 152, 161 |
| Bme18I | GGWCC | 1 cut(s) | 545 |
| BmeT110I | CYCGRG | 3 cut(s) | 126, 234, 455 |
| BmgBI | CACGTC | 1 cut(s) | 279 |
| BmgT120I | GGNCC | 2 cut(s) | 158, 545 |
| BmiI | GGNNCC | 2 cut(s) | 165, 274 |
| BmrFI | CCNGG | 2 cut(s) | 152, 161 |
| BmsI | GCATC | 2 cut(s) | 394, 469 |
| BmtI | GCTAGC | 1 cut(s) | 258 |
| Bpu14I | TTCGAA | 1 cut(s) | 347 |
| BpuMI | CCSGG | 2 cut(s) | 152, 161 |
| BsaHI | GRCGYC | 1 cut(s) | 164 |
| BsaJI | CCNNGG | 2 cut(s) | 127, 179 |
| BsaWI | WCCGGW | 3 cut(s) | 205, 388, 405 |
| BseAI | TCCGGA | 2 cut(s) | 205, 388 |
| BseDI | CCNNGG | 2 cut(s) | 127, 179 |
| BseGI | GGATG | 3 cut(s) | 160, 226, 385 |
| BseRI | GAGGAG | 1 cut(s) | 346 |
| BseXI | GCAGC | 2 cut(s) | 18, 353 |
| Bsh1236I | CGCG | 4 cut(s) | 84, 194, 464, 521 |
| BshFI | GGCC | 3 cut(s) | 159, 184, 443 |
| BshNI | GGYRCC | 1 cut(s) | 163 |
| BsiHKCI | CYCGRG | 3 cut(s) | 126, 234, 455 |
| BsiSI | CCGG | 5 cut(s) | 152, 161, 206, 389, 406 |
| BslFI | GGGAC | 1 cut(s) | 408 |
| BsmFI | GGGAC | 1 cut(s) | 408 |
| BsmI | GAATGC | 1 cut(s) | 387 |
| BsnI | GGCC | 3 cut(s) | 159, 184, 443 |
| BsoBI | CYCGRG | 3 cut(s) | 126, 234, 455 |
| Bsp119I | TTCGAA | 1 cut(s) | 347 |
| Bsp1286I | GDGCHC | 1 cut(s) | 127 |
| Bsp13I | TCCGGA | 2 cut(s) | 205, 388 |
| Bsp143I | GATC | 2 cut(s) | 7, 513 |
| Bsp68I | TCGCGA | 2 cut(s) | 84, 464 |
| BspACI | CCGC | 7 cut(s) | 4, 72, 176, 231, 258, 300, 519 |
| BspANI | GGCC | 3 cut(s) | 159, 184, 443 |
| BspEI | TCCGGA | 2 cut(s) | 205, 388 |
| BspFNI | CGCG | 4 cut(s) | 84, 194, 464, 521 |
| BspLI | GGNNCC | 2 cut(s) | 165, 274 |
| BspOI | GCTAGC | 1 cut(s) | 258 |
| BspPI | GGATC | 1 cut(s) | 15 |
| BspT104I | TTCGAA | 1 cut(s) | 347 |
| BspT107I | GGYRCC | 1 cut(s) | 163 |
| BssECI | CCNNGG | 2 cut(s) | 127, 179 |
| BssMI | GATC | 2 cut(s) | 7, 513 |
| BssNI | GRCGYC | 1 cut(s) | 164 |
| BssSI | CACGAG | 1 cut(s) | 331 |
| Bst2BI | CACGAG | 1 cut(s) | 331 |
| Bst4CI | ACNGT | 1 cut(s) | 68 |
| BstACI | GRCGYC | 1 cut(s) | 164 |
| BstBI | TTCGAA | 1 cut(s) | 347 |
| BstC8I | GCNNGC | 2 cut(s) | 82, 256 |
| BstDSI | CCRYGG | 1 cut(s) | 179 |
| BstF5I | GGATG | 3 cut(s) | 160, 226, 385 |
| BstFNI | CGCG | 4 cut(s) | 84, 194, 464, 521 |
| BstH2I | RGCGCY | 1 cut(s) | 167 |
| BstHHI | GCGC | 2 cut(s) | 166, 493 |
| BstKTI | GATC | 2 cut(s) | 10, 516 |
| BstMBI | GATC | 2 cut(s) | 7, 513 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 488 |
| BstSCI | CCNGG | 2 cut(s) | 150, 159 |
| BstUI | CGCG | 4 cut(s) | 84, 194, 464, 521 |
| BstV1I | GCAGC | 2 cut(s) | 18, 353 |
| BstX2I | RGATCY | 1 cut(s) | 7 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 68 |
| BstYI | RGATCY | 1 cut(s) | 7 |
| BsuRI | GGCC | 3 cut(s) | 159, 184, 443 |
| BtgI | CCRYGG | 1 cut(s) | 179 |
| BtgZI | GCGATG | 1 cut(s) | 99 |
| BtrI | CACGTC | 1 cut(s) | 279 |
| BtsCI | GGATG | 3 cut(s) | 160, 226, 385 |
| BtuMI | TCGCGA | 2 cut(s) | 84, 464 |
| Cac8I | GCNNGC | 2 cut(s) | 82, 256 |
| CfoI | GCGC | 2 cut(s) | 166, 493 |
| Cfr13I | GGNCC | 2 cut(s) | 158, 545 |
| Csp6I | GTAC | 2 cut(s) | 16, 403 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 420, 455 |
| CviAII | CATG | 2 cut(s) | 304, 494 |
| CviQI | GTAC | 2 cut(s) | 16, 403 |
| DinI | GGCGCC | 1 cut(s) | 165 |
| DpnI | GATC | 2 cut(s) | 9, 515 |
| DpnII | GATC | 2 cut(s) | 7, 513 |
| DraIII | CACNNNGTG | 1 cut(s) | 336 |
| EciI | GGCGGA | 2 cut(s) | 87, 165 |
| Eco24I | GRGCYC | 1 cut(s) | 127 |
| Eco47I | GGWCC | 1 cut(s) | 545 |
| Eco88I | CYCGRG | 3 cut(s) | 126, 234, 455 |
| EcoT22I | ATGCAT | 1 cut(s) | 387 |
| EcoT38I | GRGCYC | 1 cut(s) | 127 |
| EgeI | GGCGCC | 1 cut(s) | 165 |
| EheI | GGCGCC | 1 cut(s) | 165 |
| FaeI | CATG | 2 cut(s) | 307, 497 |
| FaqI | GGGAC | 1 cut(s) | 408 |
| FatI | CATG | 2 cut(s) | 303, 493 |
| FauI | CCCGC | 1 cut(s) | 238 |
| FauNDI | CATATG | 2 cut(s) | 399, 475 |
| Fnu4HI | GCNGC | 4 cut(s) | 32, 301, 342, 519 |
| FokI | GGATG | 3 cut(s) | 167, 213, 372 |
| FriOI | GRGCYC | 1 cut(s) | 127 |
| Fsp4HI | GCNGC | 4 cut(s) | 32, 301, 342, 519 |
| FspBI | CTAG | 2 cut(s) | 255, 503 |
| GlaI | GCGC | 2 cut(s) | 165, 492 |
| GluI | GCNGC | 4 cut(s) | 32, 301, 342, 519 |
| HaeII | RGCGCY | 1 cut(s) | 167 |
| HaeIII | GGCC | 3 cut(s) | 159, 184, 443 |
| HapII | CCGG | 5 cut(s) | 152, 161, 206, 389, 406 |
| HhaI | GCGC | 2 cut(s) | 166, 493 |
| Hin1I | GRCGYC | 1 cut(s) | 164 |
| Hin1II | CATG | 2 cut(s) | 307, 497 |
| Hin6I | GCGC | 2 cut(s) | 164, 491 |
| HinP1I | GCGC | 2 cut(s) | 164, 491 |
| HincII | GTYRAC | 1 cut(s) | 282 |
| HindII | GTYRAC | 1 cut(s) | 282 |
| HindIII | AAGCTT | 1 cut(s) | 51 |
| HinfI | GANTC | 3 cut(s) | 172, 307, 499 |
| HpaII | CCGG | 5 cut(s) | 152, 161, 206, 389, 406 |
| HphI | GGTGA | 2 cut(s) | 140, 541 |
| Hpy166II | GTNNAC | 1 cut(s) | 282 |
| Hpy188I | TCNGA | 1 cut(s) | 102 |
| Hpy188III | TCNNGA | 6 cut(s) | 83, 206, 331, 389, 463, 503 |
| Hpy8I | GTNNAC | 1 cut(s) | 282 |
| HpyAV | CCTTC | 1 cut(s) | 45 |
| HpyCH4III | ACNGT | 1 cut(s) | 68 |
| HpyCH4IV | ACGT | 2 cut(s) | 223, 278 |
| HpyCH4V | TGCA | 1 cut(s) | 385 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 488 |
| HpySE526I | ACGT | 2 cut(s) | 223, 278 |
| Hsp92I | GRCGYC | 1 cut(s) | 164 |
| Hsp92II | CATG | 2 cut(s) | 307, 497 |
| HspAI | GCGC | 2 cut(s) | 164, 491 |
| KasI | GGCGCC | 1 cut(s) | 163 |
| Kpn2I | TCCGGA | 2 cut(s) | 205, 388 |
| Kzo9I | GATC | 2 cut(s) | 7, 513 |
| LpnPI | CCDG | 6 cut(s) | 74, 165, 174, 219, 402, 419 |
| Lsp1109I | GCAGC | 2 cut(s) | 18, 353 |
| LweI | GCATC | 2 cut(s) | 394, 469 |
| MaeI | CTAG | 2 cut(s) | 255, 503 |
| MaeII | ACGT | 2 cut(s) | 223, 278 |
| MalI | GATC | 2 cut(s) | 9, 515 |
| MboI | GATC | 2 cut(s) | 7, 513 |
| MboII | GAAGA | 5 cut(s) | 181, 390, 535, 544, 588 |
| MflI | RGATCY | 1 cut(s) | 7 |
| MhlI | GDGCHC | 1 cut(s) | 127 |
| Mly113I | GGCGCC | 1 cut(s) | 164 |
| MlyI | GAGTC | 1 cut(s) | 181 |
| MnlI | CCTC | 6 cut(s) | 122, 327, 367, 385, 454, 564 |
| Mph1103I | ATGCAT | 1 cut(s) | 387 |
| MroI | TCCGGA | 2 cut(s) | 205, 388 |
| MseI | TTAA | 1 cut(s) | 425 |
| MslI | CAYNNNNRTG | 1 cut(s) | 153 |
| MspI | CCGG | 5 cut(s) | 152, 161, 206, 389, 406 |
| MspR9I | CCNGG | 2 cut(s) | 152, 161 |
| Mva1269I | GAATGC | 1 cut(s) | 387 |
| MvnI | CGCG | 4 cut(s) | 84, 194, 464, 521 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 488 |
| NarI | GGCGCC | 1 cut(s) | 164 |
| NciI | CCSGG | 2 cut(s) | 152, 161 |
| NdeI | CATATG | 2 cut(s) | 399, 475 |
| NdeII | GATC | 2 cut(s) | 7, 513 |
| NheI | GCTAGC | 1 cut(s) | 254 |
| NlaIII | CATG | 2 cut(s) | 307, 497 |
| NlaIV | GGNNCC | 2 cut(s) | 165, 274 |
| NruI | TCGCGA | 2 cut(s) | 84, 464 |
| NsiI | ATGCAT | 1 cut(s) | 387 |
| NspV | TTCGAA | 1 cut(s) | 347 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 176, 290 |
| PctI | GAATGC | 1 cut(s) | 387 |
| PfeI | GAWTC | 2 cut(s) | 307, 499 |
| PkrI | GCNGC | 4 cut(s) | 33, 302, 343, 520 |
| PleI | GAGTC | 1 cut(s) | 180 |
| PluTI | GGCGCC | 1 cut(s) | 167 |
| PpsI | GAGTC | 1 cut(s) | 180 |
| PspN4I | GGNNCC | 2 cut(s) | 165, 274 |
| PspPI | GGNCC | 2 cut(s) | 158, 545 |
| PsuI | RGATCY | 1 cut(s) | 7 |
| RruI | TCGCGA | 2 cut(s) | 84, 464 |
| RsaI | GTAC | 2 cut(s) | 17, 404 |
| RsaNI | GTAC | 2 cut(s) | 16, 403 |
| RseI | CAYNNNNRTG | 1 cut(s) | 153 |
| SaqAI | TTAA | 1 cut(s) | 425 |
| SatI | GCNGC | 4 cut(s) | 32, 301, 342, 519 |
| Sau3AI | GATC | 2 cut(s) | 7, 513 |
| Sau96I | GGNCC | 2 cut(s) | 158, 545 |
| SchI | GAGTC | 1 cut(s) | 181 |
| ScrFI | CCNGG | 2 cut(s) | 152, 161 |
| SduI | GDGCHC | 1 cut(s) | 127 |
| SetI | ASST | 9 cut(s) | 55, 133, 226, 256, 281, 338, 346, 431, 550 |
| SfaNI | GCATC | 2 cut(s) | 394, 469 |
| SfoI | GGCGCC | 1 cut(s) | 165 |
| SfuI | TTCGAA | 1 cut(s) | 347 |
| SinI | GGWCC | 1 cut(s) | 545 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 153 |
| SsiI | CCGC | 7 cut(s) | 4, 72, 176, 231, 258, 300, 519 |
| SspDI | GGCGCC | 1 cut(s) | 163 |
| SspMI | CTAG | 2 cut(s) | 255, 503 |
| StyD4I | CCNGG | 2 cut(s) | 150, 159 |
| TaaI | ACNGT | 1 cut(s) | 68 |
| TaiI | ACGT | 2 cut(s) | 226, 281 |
| TaqI | TCGA | 6 cut(s) | 38, 56, 121, 219, 347, 416 |
| TaqII | GACCGA | 1 cut(s) | 300 |
| TauI | GCSGC | 2 cut(s) | 303, 521 |
| TfiI | GAWTC | 2 cut(s) | 307, 499 |
| Tru1I | TTAA | 1 cut(s) | 425 |
| Tru9I | TTAA | 1 cut(s) | 425 |
| TseI | GCWGC | 2 cut(s) | 31, 341 |
| TspDTI | ATGAA | 1 cut(s) | 582 |
| VpaK11BI | GGWCC | 1 cut(s) | 545 |
| XbaI | TCTAGA | 1 cut(s) | 502 |
| XspI | CTAG | 2 cut(s) | 255, 503 |
| Zsp2I | ATGCAT | 1 cut(s) | 387 |