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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 409 |
| Acc36I | ACCTGC | 1 cut(s) | 409 |
| AccB1I | GGYRCC | 1 cut(s) | 850 |
| AccBSI | CCGCTC | 1 cut(s) | 770 |
| AccII | CGCG | 4 cut(s) | 329, 386, 776, 806 |
| AciI | CCGC | 8 cut(s) | 384, 510, 597, 653, 770, 774, 776, 858 |
| AclWI | GGATC | 2 cut(s) | 455, 488 |
| AcoI | YGGCCR | 1 cut(s) | 771 |
| AcsI | RAATTY | 1 cut(s) | 273 |
| AcyI | GRCGYC | 1 cut(s) | 851 |
| AfaI | GTAC | 3 cut(s) | 118, 335, 880 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 476 |
| AgsI | TTSAA | 2 cut(s) | 46, 262 |
| AjiI | CACGTC | 1 cut(s) | 823 |
| AjnI | CCWGG | 2 cut(s) | 86, 189 |
| AleI | CACNNNNGTG | 1 cut(s) | 32 |
| AluBI | AGCT | 8 cut(s) | 42, 135, 268, 295, 428, 443, 539, 684 |
| AluI | AGCT | 8 cut(s) | 42, 135, 268, 295, 428, 443, 539, 684 |
| Alw21I | GWGCWC | 1 cut(s) | 360 |
| Alw44I | GTGCAC | 1 cut(s) | 356 |
| AlwI | GGATC | 2 cut(s) | 455, 488 |
| AoxI | GGCC | 1 cut(s) | 771 |
| ApaLI | GTGCAC | 1 cut(s) | 356 |
| ApeKI | GCWGC | 4 cut(s) | 224, 265, 440, 443 |
| ApoI | RAATTY | 1 cut(s) | 273 |
| AspLEI | GCGC | 4 cut(s) | 130, 448, 853, 896 |
| AspS9I | GGNCC | 2 cut(s) | 187, 568 |
| AsuC2I | CCSGG | 2 cut(s) | 105, 908 |
| AsuHPI | GGTGA | 2 cut(s) | 509, 717 |
| AvaII | GGWCC | 2 cut(s) | 187, 568 |
| BaeGI | GKGCMC | 1 cut(s) | 360 |
| BanI | GGYRCC | 1 cut(s) | 850 |
| BauI | CACGAG | 1 cut(s) | 359 |
| Bbv12I | GWGCWC | 1 cut(s) | 360 |
| BbvI | GCAGC | 4 cut(s) | 211, 277, 430, 452 |
| BccI | CCATC | 4 cut(s) | 7, 174, 385, 736 |
| BceAI | ACGGC | 1 cut(s) | 453 |
| BciT130I | CCWGG | 2 cut(s) | 88, 191 |
| BcnI | CCSGG | 2 cut(s) | 105, 908 |
| BfaI | CTAG | 4 cut(s) | 449, 458, 605, 696 |
| BfoI | RGCGCY | 2 cut(s) | 131, 854 |
| BfuAI | ACCTGC | 1 cut(s) | 409 |
| BglII | AGATCT | 1 cut(s) | 454 |
| BisI | GCNGC | 8 cut(s) | 225, 266, 384, 441, 444, 598, 771, 774 |
| BlpI | GCTNAGC | 1 cut(s) | 131 |
| BlsI | GCNGC | 8 cut(s) | 226, 267, 385, 442, 445, 599, 772, 775 |
| Bme1390I | CCNGG | 4 cut(s) | 88, 105, 191, 908 |
| Bme18I | GGWCC | 2 cut(s) | 187, 568 |
| BmgBI | CACGTC | 1 cut(s) | 823 |
| BmgT120I | GGNCC | 2 cut(s) | 187, 568 |
| BmiI | GGNNCC | 2 cut(s) | 285, 852 |
| BmrFI | CCNGG | 4 cut(s) | 88, 105, 191, 908 |
| BmsI | GCATC | 4 cut(s) | 58, 58, 92, 334 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 131 |
| BpuMI | CCSGG | 2 cut(s) | 105, 908 |
| BsaAI | YACGTR | 1 cut(s) | 100 |
| BsaBI | GATNNNNATC | 1 cut(s) | 459 |
| BsaHI | GRCGYC | 1 cut(s) | 851 |
| BsaJI | CCNNGG | 4 cut(s) | 87, 279, 564, 774 |
| BsaWI | WCCGGW | 1 cut(s) | 430 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 476 |
| Bse118I | RCCGGY | 3 cut(s) | 142, 655, 847 |
| Bse8I | GATNNNNATC | 1 cut(s) | 459 |
| BseBI | CCWGG | 2 cut(s) | 88, 191 |
| BseDI | CCNNGG | 4 cut(s) | 87, 279, 564, 774 |
| BseGI | GGATG | 3 cut(s) | 83, 199, 731 |
| BseJI | GATNNNNATC | 1 cut(s) | 459 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 476 |
| BseMII | CTCAG | 3 cut(s) | 122, 312, 755 |
| BseRI | GAGGAG | 1 cut(s) | 224 |
| BseSI | GKGCMC | 1 cut(s) | 360 |
| BseX3I | CGGCCG | 1 cut(s) | 771 |
| BseXI | GCAGC | 4 cut(s) | 211, 277, 430, 452 |
| Bsh1236I | CGCG | 4 cut(s) | 329, 386, 776, 806 |
| Bsh1285I | CGRYCG | 2 cut(s) | 774, 858 |
| BshFI | GGCC | 1 cut(s) | 773 |
| BshNI | GGYRCC | 1 cut(s) | 850 |
| BsiEI | CGRYCG | 2 cut(s) | 774, 858 |
| BsiHKAI | GWGCWC | 1 cut(s) | 360 |
| BsiSI | CCGG | 6 cut(s) | 104, 143, 431, 656, 848, 908 |
| BslFI | GGGAC | 1 cut(s) | 106 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 476 |
| BsmFI | GGGAC | 1 cut(s) | 106 |
| BsmI | GAATGC | 3 cut(s) | 85, 599, 600 |
| BsnI | GGCC | 1 cut(s) | 773 |
| Bsp1286I | GDGCHC | 1 cut(s) | 360 |
| Bsp1407I | TGTACA | 1 cut(s) | 333 |
| Bsp143I | GATC | 5 cut(s) | 378, 454, 460, 480, 801 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 131 |
| Bsp68I | TCGCGA | 2 cut(s) | 329, 806 |
| BspACI | CCGC | 8 cut(s) | 384, 510, 597, 653, 770, 774, 776, 858 |
| BspANI | GGCC | 1 cut(s) | 773 |
| BspCNI | CTCAG | 3 cut(s) | 123, 313, 756 |
| BspFNI | CGCG | 4 cut(s) | 329, 386, 776, 806 |
| BspLI | GGNNCC | 2 cut(s) | 285, 852 |
| BspMI | ACCTGC | 1 cut(s) | 409 |
| BspPI | GGATC | 2 cut(s) | 455, 488 |
| BspT107I | GGYRCC | 1 cut(s) | 850 |
| BsrBI | CCGCTC | 1 cut(s) | 770 |
| BsrFI | RCCGGY | 3 cut(s) | 142, 655, 847 |
| BsrGI | TGTACA | 1 cut(s) | 333 |
| BssAI | RCCGGY | 3 cut(s) | 142, 655, 847 |
| BssECI | CCNNGG | 4 cut(s) | 87, 279, 564, 774 |
| BssMI | GATC | 5 cut(s) | 378, 454, 460, 480, 801 |
| BssNI | GRCGYC | 1 cut(s) | 851 |
| BssSI | CACGAG | 1 cut(s) | 359 |
| BssT1I | CCWWGG | 1 cut(s) | 279 |
| Bst2BI | CACGAG | 1 cut(s) | 359 |
| Bst2UI | CCWGG | 2 cut(s) | 88, 191 |
| Bst4CI | ACNGT | 1 cut(s) | 121 |
| Bst6I | CTCTTC | 4 cut(s) | 174, 201, 612, 789 |
| BstACI | GRCGYC | 1 cut(s) | 851 |
| BstAUI | TGTACA | 1 cut(s) | 333 |
| BstBAI | YACGTR | 1 cut(s) | 100 |
| BstC8I | GCNNGC | 2 cut(s) | 144, 578 |
| BstDEI | CTNAG | 4 cut(s) | 56, 131, 321, 764 |
| BstDSI | CCRYGG | 2 cut(s) | 564, 774 |
| BstF5I | GGATG | 3 cut(s) | 83, 199, 731 |
| BstFNI | CGCG | 4 cut(s) | 329, 386, 776, 806 |
| BstH2I | RGCGCY | 2 cut(s) | 131, 854 |
| BstHHI | GCGC | 4 cut(s) | 130, 448, 853, 896 |
| BstKTI | GATC | 5 cut(s) | 381, 457, 463, 483, 804 |
| BstMBI | GATC | 5 cut(s) | 378, 454, 460, 480, 801 |
| BstMCI | CGRYCG | 2 cut(s) | 774, 858 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 292, 832, 857 |
| BstNI | CCWGG | 2 cut(s) | 88, 191 |
| BstSCI | CCNGG | 4 cut(s) | 86, 103, 189, 906 |
| BstSLI | GKGCMC | 1 cut(s) | 360 |
| BstUI | CGCG | 4 cut(s) | 329, 386, 776, 806 |
| BstV1I | GCAGC | 4 cut(s) | 211, 277, 430, 452 |
| BstX2I | RGATCY | 2 cut(s) | 454, 460 |
| BstYI | RGATCY | 2 cut(s) | 454, 460 |
| BstZI | CGGCCG | 1 cut(s) | 771 |
| BsuRI | GGCC | 1 cut(s) | 773 |
| BtgI | CCRYGG | 2 cut(s) | 564, 774 |
| BtgZI | GCGATG | 1 cut(s) | 344 |
| BtrI | CACGTC | 1 cut(s) | 823 |
| BtsCI | GGATG | 3 cut(s) | 83, 199, 731 |
| BtuMI | TCGCGA | 2 cut(s) | 329, 806 |
| BveI | ACCTGC | 1 cut(s) | 409 |
| Cac8I | GCNNGC | 2 cut(s) | 144, 578 |
| CciNI | GCGGCCGC | 1 cut(s) | 771 |
| CfoI | GCGC | 4 cut(s) | 130, 448, 853, 896 |
| Cfr10I | RCCGGY | 3 cut(s) | 142, 655, 847 |
| Cfr13I | GGNCC | 2 cut(s) | 187, 568 |
| Cfr42I | CCGCGG | 1 cut(s) | 777 |
| Csp6I | GTAC | 3 cut(s) | 117, 334, 879 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 856, 891 |
| CviAII | CATG | 3 cut(s) | 487, 614, 791 |
| CviQI | GTAC | 3 cut(s) | 117, 334, 879 |
| DdeI | CTNAG | 4 cut(s) | 56, 131, 321, 764 |
| DinI | GGCGCC | 1 cut(s) | 852 |
| DpnI | GATC | 5 cut(s) | 380, 456, 462, 482, 803 |
| DpnII | GATC | 5 cut(s) | 378, 454, 460, 480, 801 |
| EaeI | YGGCCR | 1 cut(s) | 771 |
| EagI | CGGCCG | 1 cut(s) | 771 |
| Eam1104I | CTCTTC | 4 cut(s) | 174, 201, 612, 789 |
| EarI | CTCTTC | 4 cut(s) | 174, 201, 612, 789 |
| EclXI | CGGCCG | 1 cut(s) | 771 |
| Eco130I | CCWWGG | 1 cut(s) | 279 |
| Eco47I | GGWCC | 2 cut(s) | 187, 568 |
| Eco52I | CGGCCG | 1 cut(s) | 771 |
| EcoRII | CCWGG | 2 cut(s) | 86, 189 |
| EcoT14I | CCWWGG | 1 cut(s) | 279 |
| EcoT22I | ATGCAT | 1 cut(s) | 85 |
| EgeI | GGCGCC | 1 cut(s) | 852 |
| EheI | GGCGCC | 1 cut(s) | 852 |
| ErhI | CCWWGG | 1 cut(s) | 279 |
| FaeI | CATG | 3 cut(s) | 490, 617, 794 |
| FaqI | GGGAC | 1 cut(s) | 106 |
| FatI | CATG | 3 cut(s) | 486, 613, 790 |
| FauNDI | CATATG | 1 cut(s) | 340 |
| Fnu4HI | GCNGC | 8 cut(s) | 225, 266, 384, 441, 444, 598, 771, 774 |
| FokI | GGATG | 3 cut(s) | 70, 206, 718 |
| Fsp4HI | GCNGC | 8 cut(s) | 225, 266, 384, 441, 444, 598, 771, 774 |
| FspBI | CTAG | 4 cut(s) | 449, 458, 605, 696 |
| GlaI | GCGC | 4 cut(s) | 129, 447, 852, 895 |
| GluI | GCNGC | 8 cut(s) | 225, 266, 384, 441, 444, 598, 771, 774 |
| HaeII | RGCGCY | 2 cut(s) | 131, 854 |
| HaeIII | GGCC | 1 cut(s) | 773 |
| HapII | CCGG | 6 cut(s) | 104, 143, 431, 656, 848, 908 |
| HhaI | GCGC | 4 cut(s) | 130, 448, 853, 896 |
| Hin1I | GRCGYC | 1 cut(s) | 851 |
| Hin1II | CATG | 3 cut(s) | 490, 617, 794 |
| Hin6I | GCGC | 4 cut(s) | 128, 446, 851, 894 |
| HinP1I | GCGC | 4 cut(s) | 128, 446, 851, 894 |
| HinfI | GANTC | 4 cut(s) | 4, 250, 722, 743 |
| HpaII | CCGG | 6 cut(s) | 104, 143, 431, 656, 848, 908 |
| HphI | GGTGA | 2 cut(s) | 509, 717 |
| Hpy166II | GTNNAC | 3 cut(s) | 336, 358, 507 |
| Hpy188III | TCNNGA | 4 cut(s) | 328, 458, 470, 805 |
| Hpy8I | GTNNAC | 3 cut(s) | 336, 358, 507 |
| Hpy99I | CGWCG | 1 cut(s) | 518 |
| HpyAV | CCTTC | 2 cut(s) | 702, 866 |
| HpyCH4III | ACNGT | 1 cut(s) | 121 |
| HpyCH4IV | ACGT | 2 cut(s) | 99, 822 |
| HpyCH4V | TGCA | 4 cut(s) | 71, 83, 358, 418 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 292, 832, 857 |
| HpyF3I | CTNAG | 4 cut(s) | 56, 131, 321, 764 |
| HpySE526I | ACGT | 2 cut(s) | 99, 822 |
| Hsp92I | GRCGYC | 1 cut(s) | 851 |
| Hsp92II | CATG | 3 cut(s) | 490, 617, 794 |
| HspAI | GCGC | 4 cut(s) | 128, 446, 851, 894 |
| KasI | GGCGCC | 1 cut(s) | 850 |
| KroI | GCCGGC | 1 cut(s) | 142 |
| KroNI | GCCGGC | 1 cut(s) | 144 |
| KspI | CCGCGG | 1 cut(s) | 777 |
| Kzo9I | GATC | 5 cut(s) | 378, 454, 460, 480, 801 |
| LmnI | GCTCC | 4 cut(s) | 283, 433, 490, 681 |
| Lsp1109I | GCAGC | 4 cut(s) | 211, 277, 430, 452 |
| LweI | GCATC | 4 cut(s) | 58, 58, 92, 334 |
| MaeI | CTAG | 4 cut(s) | 449, 458, 605, 696 |
| MaeII | ACGT | 2 cut(s) | 99, 822 |
| MaeIII | GTNAC | 2 cut(s) | 433, 723 |
| MalI | GATC | 5 cut(s) | 380, 456, 462, 482, 803 |
| MbiI | CCGCTC | 1 cut(s) | 770 |
| MboI | GATC | 5 cut(s) | 378, 454, 460, 480, 801 |
| MboII | GAAGA | 8 cut(s) | 88, 191, 218, 400, 602, 629, 806, 811 |
| MfeI | CAATTG | 1 cut(s) | 919 |
| MflI | RGATCY | 2 cut(s) | 454, 460 |
| MhlI | GDGCHC | 1 cut(s) | 360 |
| MluCI | AATT | 4 cut(s) | 273, 887, 901, 919 |
| Mly113I | GGCGCC | 1 cut(s) | 851 |
| MlyI | GAGTC | 2 cut(s) | 731, 737 |
| MnlI | CCTC | 6 cut(s) | 25, 175, 196, 202, 568, 613 |
| Mph1103I | ATGCAT | 1 cut(s) | 85 |
| MroNI | GCCGGC | 1 cut(s) | 142 |
| MseI | TTAA | 3 cut(s) | 236, 402, 890 |
| MslI | CAYNNNNRTG | 3 cut(s) | 32, 174, 789 |
| MspA1I | CMGCKG | 3 cut(s) | 268, 443, 776 |
| MspI | CCGG | 6 cut(s) | 104, 143, 431, 656, 848, 908 |
| MspR9I | CCNGG | 4 cut(s) | 88, 105, 191, 908 |
| MunI | CAATTG | 1 cut(s) | 919 |
| Mva1269I | GAATGC | 3 cut(s) | 85, 599, 600 |
| MvaI | CCWGG | 2 cut(s) | 88, 191 |
| MvnI | CGCG | 4 cut(s) | 329, 386, 776, 806 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 292, 832, 857 |
| NaeI | GCCGGC | 1 cut(s) | 144 |
| NarI | GGCGCC | 1 cut(s) | 851 |
| NciI | CCSGG | 2 cut(s) | 105, 908 |
| NdeI | CATATG | 1 cut(s) | 340 |
| NdeII | GATC | 5 cut(s) | 378, 454, 460, 480, 801 |
| NgoMIV | GCCGGC | 1 cut(s) | 142 |
| NlaIII | CATG | 3 cut(s) | 490, 617, 794 |
| NlaIV | GGNNCC | 2 cut(s) | 285, 852 |
| NmuCI | GTSAC | 2 cut(s) | 433, 723 |
| NotI | GCGGCCGC | 1 cut(s) | 771 |
| NruI | TCGCGA | 2 cut(s) | 329, 806 |
| NsiI | ATGCAT | 1 cut(s) | 85 |
| OliI | CACNNNNGTG | 1 cut(s) | 32 |
| PaqCI | CACCTGC | 1 cut(s) | 409 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 228, 366, 522 |
| PctI | GAATGC | 3 cut(s) | 85, 599, 600 |
| PdiI | GCCGGC | 1 cut(s) | 144 |
| PfeI | GAWTC | 2 cut(s) | 4, 250 |
| PfoI | TCCNGGA | 1 cut(s) | 189 |
| PkrI | GCNGC | 8 cut(s) | 226, 267, 385, 442, 445, 599, 772, 775 |
| PleI | GAGTC | 2 cut(s) | 730, 737 |
| PluTI | GGCGCC | 1 cut(s) | 854 |
| PpsI | GAGTC | 2 cut(s) | 730, 737 |
| Ppu21I | YACGTR | 1 cut(s) | 100 |
| Psp6I | CCWGG | 2 cut(s) | 86, 189 |
| PspGI | CCWGG | 2 cut(s) | 86, 189 |
| PspN4I | GGNNCC | 2 cut(s) | 285, 852 |
| PspPI | GGNCC | 2 cut(s) | 187, 568 |
| PsuI | RGATCY | 2 cut(s) | 454, 460 |
| PvuII | CAGCTG | 2 cut(s) | 268, 443 |
| RruI | TCGCGA | 2 cut(s) | 329, 806 |
| RsaI | GTAC | 3 cut(s) | 118, 335, 880 |
| RsaNI | GTAC | 3 cut(s) | 117, 334, 879 |
| RseI | CAYNNNNRTG | 3 cut(s) | 32, 174, 789 |
| SacII | CCGCGG | 1 cut(s) | 777 |
| SaqAI | TTAA | 3 cut(s) | 236, 402, 890 |
| SatI | GCNGC | 8 cut(s) | 225, 266, 384, 441, 444, 598, 771, 774 |
| Sau3AI | GATC | 5 cut(s) | 378, 454, 460, 480, 801 |
| Sau96I | GGNCC | 2 cut(s) | 187, 568 |
| SchI | GAGTC | 2 cut(s) | 731, 737 |
| ScrFI | CCNGG | 4 cut(s) | 88, 105, 191, 908 |
| SduI | GDGCHC | 1 cut(s) | 360 |
| SfaNI | GCATC | 4 cut(s) | 58, 58, 92, 334 |
| SfoI | GGCGCC | 1 cut(s) | 852 |
| Sfr303I | CCGCGG | 1 cut(s) | 777 |
| SgrAI | CRCCGGYG | 1 cut(s) | 847 |
| SgrBI | CCGCGG | 1 cut(s) | 777 |
| SinI | GGWCC | 2 cut(s) | 187, 568 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 32, 174, 789 |
| Sse9I | AATT | 4 cut(s) | 273, 887, 901, 919 |
| SsiI | CCGC | 8 cut(s) | 384, 510, 597, 653, 770, 774, 776, 858 |
| SspDI | GGCGCC | 1 cut(s) | 850 |
| SspMI | CTAG | 4 cut(s) | 449, 458, 605, 696 |
| StyD4I | CCNGG | 4 cut(s) | 86, 103, 189, 906 |
| StyI | CCWWGG | 1 cut(s) | 279 |
| TaaI | ACNGT | 1 cut(s) | 121 |
| TaiI | ACGT | 2 cut(s) | 102, 825 |
| TaqI | TCGA | 3 cut(s) | 11, 369, 624 |
| TasI | AATT | 4 cut(s) | 273, 887, 901, 919 |
| TatI | WGTACW | 1 cut(s) | 333 |
| TauI | GCSGC | 4 cut(s) | 386, 600, 773, 776 |
| TfiI | GAWTC | 2 cut(s) | 4, 250 |
| Tru1I | TTAA | 3 cut(s) | 236, 402, 890 |
| Tru9I | TTAA | 3 cut(s) | 236, 402, 890 |
| TseFI | GTSAC | 2 cut(s) | 433, 723 |
| TseI | GCWGC | 4 cut(s) | 224, 265, 440, 443 |
| Tsp45I | GTSAC | 2 cut(s) | 433, 723 |
| TspDTI | ATGAA | 3 cut(s) | 192, 630, 807 |
| VneI | GTGCAC | 1 cut(s) | 356 |
| VpaK11BI | GGWCC | 2 cut(s) | 187, 568 |
| XapI | RAATTY | 1 cut(s) | 273 |
| XbaI | TCTAGA | 1 cut(s) | 457 |
| XspI | CTAG | 4 cut(s) | 449, 458, 605, 696 |
| Zsp2I | ATGCAT | 1 cut(s) | 85 |