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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 175 |
| AccII | CGCG | 1 cut(s) | 298 |
| AciI | CCGC | 3 cut(s) | 33, 175, 731 |
| AclWI | GGATC | 3 cut(s) | 542, 555, 770 |
| AfaI | GTAC | 1 cut(s) | 329 |
| AflIII | ACRYGT | 1 cut(s) | 324 |
| AgeI | ACCGGT | 1 cut(s) | 374 |
| AgsI | TTSAA | 5 cut(s) | 191, 287, 532, 585, 761 |
| AjnI | CCWGG | 3 cut(s) | 339, 513, 693 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 524, 556 |
| AluBI | AGCT | 3 cut(s) | 358, 503, 539 |
| AluI | AGCT | 3 cut(s) | 358, 503, 539 |
| Alw26I | GTCTC | 1 cut(s) | 113 |
| AlwI | GGATC | 3 cut(s) | 542, 555, 770 |
| AoxI | GGCC | 2 cut(s) | 381, 714 |
| ApeKI | GCWGC | 1 cut(s) | 203 |
| AsiGI | ACCGGT | 1 cut(s) | 374 |
| AspLEI | GCGC | 1 cut(s) | 246 |
| AspS9I | GGNCC | 1 cut(s) | 511 |
| AsuNHI | GCTAGC | 1 cut(s) | 77 |
| AvaII | GGWCC | 1 cut(s) | 511 |
| BaeGI | GKGCMC | 1 cut(s) | 141 |
| BamHI | GGATCC | 1 cut(s) | 547 |
| BauI | CACGAG | 1 cut(s) | 71 |
| BbsI | GAAGAC | 2 cut(s) | 629, 758 |
| BbvI | GCAGC | 1 cut(s) | 190 |
| BccI | CCATC | 2 cut(s) | 214, 655 |
| BceAI | ACGGC | 1 cut(s) | 368 |
| BciT130I | CCWGG | 3 cut(s) | 341, 515, 695 |
| BcoDI | GTCTC | 1 cut(s) | 113 |
| BfaI | CTAG | 4 cut(s) | 78, 359, 627, 702 |
| BfmI | CTRYAG | 1 cut(s) | 651 |
| BfoI | RGCGCY | 1 cut(s) | 247 |
| BisI | GCNGC | 2 cut(s) | 176, 204 |
| BlsI | GCNGC | 2 cut(s) | 177, 205 |
| Bme1390I | CCNGG | 3 cut(s) | 341, 515, 695 |
| Bme18I | GGWCC | 1 cut(s) | 511 |
| BmgT120I | GGNCC | 1 cut(s) | 511 |
| BmiI | GGNNCC | 1 cut(s) | 549 |
| BmrFI | CCNGG | 3 cut(s) | 341, 515, 695 |
| BmsI | GCATC | 3 cut(s) | 95, 446, 458 |
| BmtI | GCTAGC | 1 cut(s) | 81 |
| BoxI | GACNNNNGTC | 1 cut(s) | 485 |
| BpiI | GAAGAC | 2 cut(s) | 629, 758 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 730, 762 |
| BpmI | CTGGAG | 1 cut(s) | 638 |
| BsaJI | CCNNGG | 1 cut(s) | 693 |
| BsaWI | WCCGGW | 1 cut(s) | 374 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 709, 739 |
| Bse118I | RCCGGY | 1 cut(s) | 374 |
| BseBI | CCWGG | 3 cut(s) | 341, 515, 695 |
| BseDI | CCNNGG | 1 cut(s) | 693 |
| BseGI | GGATG | 3 cut(s) | 86, 225, 473 |
| BseMII | CTCAG | 3 cut(s) | 30, 495, 681 |
| BseRI | GAGGAG | 2 cut(s) | 131, 185 |
| BseSI | GKGCMC | 1 cut(s) | 141 |
| BseXI | GCAGC | 1 cut(s) | 190 |
| BseYI | CCCAGC | 2 cut(s) | 27, 151 |
| Bsh1236I | CGCG | 1 cut(s) | 298 |
| BshFI | GGCC | 2 cut(s) | 383, 716 |
| BshTI | ACCGGT | 1 cut(s) | 374 |
| BsiSI | CCGG | 1 cut(s) | 375 |
| BslFI | GGGAC | 1 cut(s) | 324 |
| BsmAI | GTCTC | 1 cut(s) | 113 |
| BsmFI | GGGAC | 1 cut(s) | 324 |
| BsmI | GAATGC | 1 cut(s) | 163 |
| BsnI | GGCC | 2 cut(s) | 383, 716 |
| Bsp1286I | GDGCHC | 1 cut(s) | 141 |
| Bsp1407I | TGTACA | 1 cut(s) | 327 |
| Bsp143I | GATC | 4 cut(s) | 267, 293, 547, 775 |
| Bsp68I | TCGCGA | 1 cut(s) | 298 |
| BspACI | CCGC | 3 cut(s) | 33, 175, 731 |
| BspANI | GGCC | 2 cut(s) | 383, 716 |
| BspCNI | CTCAG | 3 cut(s) | 31, 496, 680 |
| BspFNI | CGCG | 1 cut(s) | 298 |
| BspLI | GGNNCC | 1 cut(s) | 549 |
| BspMAI | CTGCAG | 1 cut(s) | 655 |
| BspOI | GCTAGC | 1 cut(s) | 81 |
| BspPI | GGATC | 3 cut(s) | 542, 555, 770 |
| BspQI | GCTCTTC | 1 cut(s) | 234 |
| BsrBI | CCGCTC | 1 cut(s) | 175 |
| BsrFI | RCCGGY | 1 cut(s) | 374 |
| BsrGI | TGTACA | 1 cut(s) | 327 |
| BssAI | RCCGGY | 1 cut(s) | 374 |
| BssECI | CCNNGG | 1 cut(s) | 693 |
| BssMI | GATC | 4 cut(s) | 267, 293, 547, 775 |
| BssSI | CACGAG | 1 cut(s) | 71 |
| Bst2BI | CACGAG | 1 cut(s) | 71 |
| Bst2UI | CCWGG | 3 cut(s) | 341, 515, 695 |
| Bst4CI | ACNGT | 2 cut(s) | 640, 757 |
| Bst6I | CTCTTC | 1 cut(s) | 234 |
| BstAUI | TGTACA | 1 cut(s) | 327 |
| BstC8I | GCNNGC | 5 cut(s) | 33, 79, 163, 180, 395 |
| BstDEI | CTNAG | 3 cut(s) | 39, 504, 667 |
| BstF5I | GGATG | 3 cut(s) | 86, 225, 473 |
| BstFNI | CGCG | 1 cut(s) | 298 |
| BstH2I | RGCGCY | 1 cut(s) | 247 |
| BstHHI | GCGC | 1 cut(s) | 246 |
| BstKTI | GATC | 4 cut(s) | 270, 296, 550, 778 |
| BstMAI | GTCTC | 1 cut(s) | 113 |
| BstMBI | GATC | 4 cut(s) | 267, 293, 547, 775 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 83, 250, 304 |
| BstNI | CCWGG | 3 cut(s) | 341, 515, 695 |
| BstNSI | RCATGY | 1 cut(s) | 328 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 485 |
| BstSCI | CCNGG | 3 cut(s) | 339, 513, 693 |
| BstSFI | CTRYAG | 1 cut(s) | 651 |
| BstSLI | GKGCMC | 1 cut(s) | 141 |
| BstUI | CGCG | 1 cut(s) | 298 |
| BstV1I | GCAGC | 1 cut(s) | 190 |
| BstV2I | GAAGAC | 2 cut(s) | 629, 758 |
| BstX2I | RGATCY | 1 cut(s) | 547 |
| BstYI | RGATCY | 1 cut(s) | 547 |
| BsuRI | GGCC | 2 cut(s) | 383, 716 |
| BtsCI | GGATG | 3 cut(s) | 86, 225, 473 |
| BtsI | GCAGTG | 1 cut(s) | 93 |
| BtsIMutI | CAGTG | 3 cut(s) | 93, 272, 636 |
| BtuMI | TCGCGA | 1 cut(s) | 298 |
| Cac8I | GCNNGC | 5 cut(s) | 33, 79, 163, 180, 395 |
| CfoI | GCGC | 1 cut(s) | 246 |
| Cfr10I | RCCGGY | 1 cut(s) | 374 |
| Cfr13I | GGNCC | 1 cut(s) | 511 |
| Csp6I | GTAC | 1 cut(s) | 328 |
| CspAI | ACCGGT | 1 cut(s) | 374 |
| CviAII | CATG | 2 cut(s) | 325, 493 |
| CviQI | GTAC | 1 cut(s) | 328 |
| DdeI | CTNAG | 3 cut(s) | 39, 504, 667 |
| DpnI | GATC | 4 cut(s) | 269, 295, 549, 777 |
| DpnII | GATC | 4 cut(s) | 267, 293, 547, 775 |
| Eam1104I | CTCTTC | 1 cut(s) | 234 |
| EarI | CTCTTC | 1 cut(s) | 234 |
| Eco47I | GGWCC | 1 cut(s) | 511 |
| EcoRII | CCWGG | 3 cut(s) | 339, 513, 693 |
| FaeI | CATG | 2 cut(s) | 328, 496 |
| FaiI | YATR | 7 cut(s) | 208, 210, 326, 368, 432, 494, 602 |
| FaqI | GGGAC | 1 cut(s) | 324 |
| FatI | CATG | 2 cut(s) | 324, 492 |
| FauI | CCCGC | 2 cut(s) | 40, 738 |
| Fnu4HI | GCNGC | 2 cut(s) | 176, 204 |
| FokI | GGATG | 3 cut(s) | 73, 232, 480 |
| Fsp4HI | GCNGC | 2 cut(s) | 176, 204 |
| FspBI | CTAG | 4 cut(s) | 78, 359, 627, 702 |
| GlaI | GCGC | 1 cut(s) | 245 |
| GluI | GCNGC | 2 cut(s) | 176, 204 |
| GsaI | CCCAGC | 2 cut(s) | 31, 155 |
| GsuI | CTGGAG | 1 cut(s) | 638 |
| HaeII | RGCGCY | 1 cut(s) | 247 |
| HaeIII | GGCC | 2 cut(s) | 383, 716 |
| HapII | CCGG | 1 cut(s) | 375 |
| HhaI | GCGC | 1 cut(s) | 246 |
| Hin1II | CATG | 2 cut(s) | 328, 496 |
| Hin6I | GCGC | 1 cut(s) | 244 |
| HinP1I | GCGC | 1 cut(s) | 244 |
| HincII | GTYRAC | 1 cut(s) | 6 |
| HindII | GTYRAC | 1 cut(s) | 6 |
| HindIII | AAGCTT | 1 cut(s) | 537 |
| HinfI | GANTC | 2 cut(s) | 107, 745 |
| HpaII | CCGG | 1 cut(s) | 375 |
| Hpy166II | GTNNAC | 3 cut(s) | 6, 418, 789 |
| Hpy188I | TCNGA | 3 cut(s) | 390, 442, 750 |
| Hpy188III | TCNNGA | 6 cut(s) | 71, 284, 297, 415, 529, 617 |
| Hpy8I | GTNNAC | 3 cut(s) | 6, 418, 789 |
| HpyAV | CCTTC | 4 cut(s) | 243, 464, 591, 755 |
| HpyCH4III | ACNGT | 2 cut(s) | 640, 757 |
| HpyCH4V | TGCA | 5 cut(s) | 206, 437, 471, 496, 653 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 83, 250, 304 |
| HpyF3I | CTNAG | 3 cut(s) | 39, 504, 667 |
| Hsp92II | CATG | 2 cut(s) | 328, 496 |
| HspAI | GCGC | 1 cut(s) | 244 |
| Kzo9I | GATC | 4 cut(s) | 267, 293, 547, 775 |
| LguI | GCTCTTC | 1 cut(s) | 234 |
| LmnI | GCTCC | 1 cut(s) | 172 |
| Lsp1109I | GCAGC | 1 cut(s) | 190 |
| LweI | GCATC | 3 cut(s) | 95, 446, 458 |
| MaeI | CTAG | 4 cut(s) | 78, 359, 627, 702 |
| MaeIII | GTNAC | 4 cut(s) | 640, 670, 697, 707 |
| MalI | GATC | 4 cut(s) | 269, 295, 549, 777 |
| MbiI | CCGCTC | 1 cut(s) | 175 |
| MboI | GATC | 4 cut(s) | 267, 293, 547, 775 |
| MboII | GAAGA | 4 cut(s) | 47, 251, 634, 763 |
| MflI | RGATCY | 1 cut(s) | 547 |
| MhlI | GDGCHC | 1 cut(s) | 141 |
| MluCI | AATT | 2 cut(s) | 127, 443 |
| MlyI | GAGTC | 1 cut(s) | 739 |
| MmeI | TCCRAC | 3 cut(s) | 88, 114, 489 |
| MspI | CCGG | 1 cut(s) | 375 |
| MspR9I | CCNGG | 3 cut(s) | 341, 515, 695 |
| Mva1269I | GAATGC | 1 cut(s) | 163 |
| MvaI | CCWGG | 3 cut(s) | 341, 515, 695 |
| MvnI | CGCG | 1 cut(s) | 298 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 83, 250, 304 |
| NdeII | GATC | 4 cut(s) | 267, 293, 547, 775 |
| NheI | GCTAGC | 1 cut(s) | 77 |
| NlaIII | CATG | 2 cut(s) | 328, 496 |
| NlaIV | GGNNCC | 1 cut(s) | 549 |
| NruI | TCGCGA | 1 cut(s) | 298 |
| NspI | RCATGY | 1 cut(s) | 328 |
| PciI | ACATGT | 1 cut(s) | 324 |
| PciSI | GCTCTTC | 1 cut(s) | 234 |
| PctI | GAATGC | 1 cut(s) | 163 |
| PfeI | GAWTC | 1 cut(s) | 107 |
| PinAI | ACCGGT | 1 cut(s) | 374 |
| PkrI | GCNGC | 2 cut(s) | 177, 205 |
| PleI | GAGTC | 1 cut(s) | 739 |
| PpsI | GAGTC | 1 cut(s) | 739 |
| PscI | ACATGT | 1 cut(s) | 324 |
| PshAI | GACNNNNGTC | 1 cut(s) | 485 |
| Psp6I | CCWGG | 3 cut(s) | 339, 513, 693 |
| PspFI | CCCAGC | 2 cut(s) | 27, 151 |
| PspGI | CCWGG | 3 cut(s) | 339, 513, 693 |
| PspN4I | GGNNCC | 1 cut(s) | 549 |
| PspPI | GGNCC | 1 cut(s) | 511 |
| PstI | CTGCAG | 1 cut(s) | 655 |
| PsuI | RGATCY | 1 cut(s) | 547 |
| RruI | TCGCGA | 1 cut(s) | 298 |
| RsaI | GTAC | 1 cut(s) | 329 |
| RsaNI | GTAC | 1 cut(s) | 328 |
| SapI | GCTCTTC | 1 cut(s) | 234 |
| SatI | GCNGC | 2 cut(s) | 176, 204 |
| Sau3AI | GATC | 4 cut(s) | 267, 293, 547, 775 |
| Sau96I | GGNCC | 1 cut(s) | 511 |
| SchI | GAGTC | 1 cut(s) | 739 |
| ScrFI | CCNGG | 3 cut(s) | 341, 515, 695 |
| SduI | GDGCHC | 1 cut(s) | 141 |
| SetI | ASST | 9 cut(s) | 70, 264, 360, 505, 528, 541, 583, 607, 727 |
| SfaNI | GCATC | 3 cut(s) | 95, 446, 458 |
| SfcI | CTRYAG | 1 cut(s) | 651 |
| SinI | GGWCC | 1 cut(s) | 511 |
| Sse9I | AATT | 2 cut(s) | 127, 443 |
| SsiI | CCGC | 3 cut(s) | 33, 175, 731 |
| SspMI | CTAG | 4 cut(s) | 78, 359, 627, 702 |
| StyD4I | CCNGG | 3 cut(s) | 339, 513, 693 |
| TaaI | ACNGT | 2 cut(s) | 640, 757 |
| TaqI | TCGA | 2 cut(s) | 110, 266 |
| TasI | AATT | 2 cut(s) | 127, 443 |
| TatI | WGTACW | 1 cut(s) | 327 |
| TauI | GCSGC | 1 cut(s) | 178 |
| TfiI | GAWTC | 1 cut(s) | 107 |
| TscAI | CASTG | 3 cut(s) | 100, 279, 643 |
| TseI | GCWGC | 1 cut(s) | 203 |
| TspDTI | ATGAA | 1 cut(s) | 228 |
| TspRI | CASTG | 3 cut(s) | 100, 279, 643 |
| VpaK11BI | GGWCC | 1 cut(s) | 511 |
| XceI | RCATGY | 1 cut(s) | 328 |
| XspI | CTAG | 4 cut(s) | 78, 359, 627, 702 |