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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 79 |
| AciI | CCGC | 1 cut(s) | 154 |
| AclWI | GGATC | 2 cut(s) | 217, 451 |
| AfaI | GTAC | 2 cut(s) | 435, 471 |
| AfeI | AGCGCT | 1 cut(s) | 418 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 511 |
| AgsI | TTSAA | 3 cut(s) | 199, 239, 476 |
| AjiI | CACGTC | 2 cut(s) | 77, 293 |
| AluBI | AGCT | 5 cut(s) | 89, 170, 354, 383, 487 |
| AluI | AGCT | 5 cut(s) | 89, 170, 354, 383, 487 |
| Alw21I | GWGCWC | 3 cut(s) | 172, 356, 610 |
| Alw26I | GTCTC | 2 cut(s) | 96, 566 |
| AlwI | GGATC | 2 cut(s) | 217, 451 |
| Aor51HI | AGCGCT | 1 cut(s) | 418 |
| AoxI | GGCC | 1 cut(s) | 151 |
| ApeKI | GCWGC | 1 cut(s) | 89 |
| AseI | ATTAAT | 1 cut(s) | 45 |
| AspLEI | GCGC | 1 cut(s) | 419 |
| AspS9I | GGNCC | 1 cut(s) | 327 |
| AsuC2I | CCSGG | 2 cut(s) | 325, 506 |
| AsuHPI | GGTGA | 2 cut(s) | 173, 216 |
| AvaII | GGWCC | 1 cut(s) | 327 |
| BanII | GRGCYC | 2 cut(s) | 172, 356 |
| BauI | CACGAG | 1 cut(s) | 171 |
| Bbv12I | GWGCWC | 3 cut(s) | 172, 356, 610 |
| BbvI | GCAGC | 1 cut(s) | 76 |
| BccI | CCATC | 1 cut(s) | 104 |
| BceAI | ACGGC | 1 cut(s) | 528 |
| BcnI | CCSGG | 2 cut(s) | 325, 506 |
| BcoDI | GTCTC | 2 cut(s) | 96, 566 |
| BfaI | CTAG | 1 cut(s) | 372 |
| BfoI | RGCGCY | 1 cut(s) | 420 |
| BglII | AGATCT | 1 cut(s) | 36 |
| BisI | GCNGC | 2 cut(s) | 90, 154 |
| BlsI | GCNGC | 2 cut(s) | 91, 155 |
| Bme1390I | CCNGG | 2 cut(s) | 325, 506 |
| Bme18I | GGWCC | 1 cut(s) | 327 |
| BmgBI | CACGTC | 2 cut(s) | 77, 293 |
| BmgT120I | GGNCC | 1 cut(s) | 327 |
| BmiI | GGNNCC | 1 cut(s) | 328 |
| BmrFI | CCNGG | 2 cut(s) | 325, 506 |
| BmrI | ACTGGG | 1 cut(s) | 420 |
| BmsI | GCATC | 1 cut(s) | 61 |
| BmuI | ACTGGG | 1 cut(s) | 420 |
| BpuEI | CTTGAG | 1 cut(s) | 349 |
| BpuMI | CCSGG | 2 cut(s) | 325, 506 |
| Bsa29I | ATCGAT | 1 cut(s) | 409 |
| BsaJI | CCNNGG | 1 cut(s) | 504 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 511 |
| Bse1I | ACTGG | 2 cut(s) | 65, 426 |
| BseCI | ATCGAT | 1 cut(s) | 409 |
| BseDI | CCNNGG | 1 cut(s) | 504 |
| BseGI | GGATG | 2 cut(s) | 10, 518 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 511 |
| BseMII | CTCAG | 1 cut(s) | 479 |
| BseNI | ACTGG | 2 cut(s) | 65, 426 |
| BseXI | GCAGC | 1 cut(s) | 76 |
| BsgI | GTGCAG | 1 cut(s) | 135 |
| Bsh1285I | CGRYCG | 1 cut(s) | 409 |
| BshFI | GGCC | 1 cut(s) | 153 |
| BshVI | ATCGAT | 1 cut(s) | 409 |
| BsiEI | CGRYCG | 1 cut(s) | 409 |
| BsiHKAI | GWGCWC | 3 cut(s) | 172, 356, 610 |
| BsiSI | CCGG | 2 cut(s) | 324, 506 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 511 |
| BsmAI | GTCTC | 2 cut(s) | 96, 566 |
| BsmBI | CGTCTC | 1 cut(s) | 566 |
| BsnI | GGCC | 1 cut(s) | 153 |
| Bsp1286I | GDGCHC | 3 cut(s) | 172, 356, 610 |
| Bsp143I | GATC | 7 cut(s) | 36, 96, 135, 209, 406, 443, 525 |
| BspACI | CCGC | 1 cut(s) | 154 |
| BspANI | GGCC | 1 cut(s) | 153 |
| BspCNI | CTCAG | 1 cut(s) | 480 |
| BspDI | ATCGAT | 1 cut(s) | 409 |
| BspLI | GGNNCC | 1 cut(s) | 328 |
| BspPI | GGATC | 2 cut(s) | 217, 451 |
| BspQI | GCTCTTC | 2 cut(s) | 426, 454 |
| BsrI | ACTGG | 2 cut(s) | 65, 426 |
| BssECI | CCNNGG | 1 cut(s) | 504 |
| BssMI | GATC | 7 cut(s) | 36, 96, 135, 209, 406, 443, 525 |
| BssSI | CACGAG | 1 cut(s) | 171 |
| Bst2BI | CACGAG | 1 cut(s) | 171 |
| Bst6I | CTCTTC | 3 cut(s) | 226, 426, 454 |
| BstC8I | GCNNGC | 2 cut(s) | 352, 501 |
| BstDEI | CTNAG | 1 cut(s) | 488 |
| BstEII | GGTNACC | 1 cut(s) | 161 |
| BstF5I | GGATG | 2 cut(s) | 10, 518 |
| BstH2I | RGCGCY | 1 cut(s) | 420 |
| BstHHI | GCGC | 1 cut(s) | 419 |
| BstKTI | GATC | 7 cut(s) | 39, 99, 138, 212, 409, 446, 528 |
| BstMAI | GTCTC | 2 cut(s) | 96, 566 |
| BstMBI | GATC | 7 cut(s) | 36, 96, 135, 209, 406, 443, 525 |
| BstMCI | CGRYCG | 1 cut(s) | 409 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 347 |
| BstNSI | RCATGY | 1 cut(s) | 503 |
| BstPI | GGTNACC | 1 cut(s) | 161 |
| BstSCI | CCNGG | 2 cut(s) | 323, 504 |
| BstV1I | GCAGC | 1 cut(s) | 76 |
| BstX2I | RGATCY | 1 cut(s) | 36 |
| BstYI | RGATCY | 1 cut(s) | 36 |
| Bsu15I | ATCGAT | 1 cut(s) | 409 |
| BsuRI | GGCC | 1 cut(s) | 153 |
| BsuTUI | ATCGAT | 1 cut(s) | 409 |
| BtrI | CACGTC | 2 cut(s) | 77, 293 |
| BtsCI | GGATG | 2 cut(s) | 10, 518 |
| BtsI | GCAGTG | 1 cut(s) | 123 |
| BtsIMutI | CAGTG | 1 cut(s) | 123 |
| Cac8I | GCNNGC | 2 cut(s) | 352, 501 |
| CfoI | GCGC | 1 cut(s) | 419 |
| Cfr13I | GGNCC | 1 cut(s) | 327 |
| ClaI | ATCGAT | 1 cut(s) | 409 |
| Csp6I | GTAC | 2 cut(s) | 434, 470 |
| CviAII | CATG | 2 cut(s) | 260, 500 |
| CviQI | GTAC | 2 cut(s) | 434, 470 |
| DdeI | CTNAG | 1 cut(s) | 488 |
| DpnI | GATC | 7 cut(s) | 38, 98, 137, 211, 408, 445, 527 |
| DpnII | GATC | 7 cut(s) | 36, 96, 135, 209, 406, 443, 525 |
| Eam1104I | CTCTTC | 3 cut(s) | 226, 426, 454 |
| EarI | CTCTTC | 3 cut(s) | 226, 426, 454 |
| Ecl136II | GAGCTC | 2 cut(s) | 170, 354 |
| Eco24I | GRGCYC | 2 cut(s) | 172, 356 |
| Eco47I | GGWCC | 1 cut(s) | 327 |
| Eco47III | AGCGCT | 1 cut(s) | 418 |
| Eco53kI | GAGCTC | 2 cut(s) | 170, 354 |
| Eco91I | GGTNACC | 1 cut(s) | 161 |
| EcoICRI | GAGCTC | 2 cut(s) | 170, 354 |
| EcoO109I | RGGNCCY | 1 cut(s) | 327 |
| EcoO65I | GGTNACC | 1 cut(s) | 161 |
| EcoT38I | GRGCYC | 2 cut(s) | 172, 356 |
| Esp3I | CGTCTC | 1 cut(s) | 566 |
| FaeI | CATG | 2 cut(s) | 263, 503 |
| FaiI | YATR | 7 cut(s) | 159, 255, 261, 276, 468, 501, 530 |
| FatI | CATG | 2 cut(s) | 259, 499 |
| FblI | GTMKAC | 1 cut(s) | 79 |
| Fnu4HI | GCNGC | 2 cut(s) | 90, 154 |
| FokI | GGATG | 2 cut(s) | 17, 505 |
| FriOI | GRGCYC | 2 cut(s) | 172, 356 |
| Fsp4HI | GCNGC | 2 cut(s) | 90, 154 |
| FspBI | CTAG | 1 cut(s) | 372 |
| GlaI | GCGC | 1 cut(s) | 418 |
| GluI | GCNGC | 2 cut(s) | 90, 154 |
| HaeII | RGCGCY | 1 cut(s) | 420 |
| HaeIII | GGCC | 1 cut(s) | 153 |
| HapII | CCGG | 2 cut(s) | 324, 506 |
| HhaI | GCGC | 1 cut(s) | 419 |
| Hin1II | CATG | 2 cut(s) | 263, 503 |
| Hin6I | GCGC | 1 cut(s) | 417 |
| HinP1I | GCGC | 1 cut(s) | 417 |
| HinfI | GANTC | 2 cut(s) | 104, 244 |
| HpaII | CCGG | 2 cut(s) | 324, 506 |
| HphI | GGTGA | 2 cut(s) | 173, 216 |
| Hpy166II | GTNNAC | 1 cut(s) | 80 |
| Hpy188I | TCNGA | 5 cut(s) | 101, 109, 135, 214, 579 |
| Hpy188III | TCNNGA | 1 cut(s) | 40 |
| Hpy8I | GTNNAC | 1 cut(s) | 80 |
| Hpy99I | CGWCG | 2 cut(s) | 97, 597 |
| HpyCH4IV | ACGT | 3 cut(s) | 76, 292, 436 |
| HpyCH4V | TGCA | 2 cut(s) | 74, 116 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 347 |
| HpyF3I | CTNAG | 1 cut(s) | 488 |
| HpySE526I | ACGT | 3 cut(s) | 76, 292, 436 |
| Hsp92II | CATG | 2 cut(s) | 263, 503 |
| HspAI | GCGC | 1 cut(s) | 417 |
| Kzo9I | GATC | 7 cut(s) | 36, 96, 135, 209, 406, 443, 525 |
| LguI | GCTCTTC | 2 cut(s) | 426, 454 |
| LmnI | GCTCC | 2 cut(s) | 359, 613 |
| LpnPI | CCDG | 9 cut(s) | 78, 337, 375, 393, 416, 439, 519, 522, 526 |
| Lsp1109I | GCAGC | 1 cut(s) | 76 |
| LweI | GCATC | 1 cut(s) | 61 |
| MaeI | CTAG | 1 cut(s) | 372 |
| MaeII | ACGT | 3 cut(s) | 76, 292, 436 |
| MaeIII | GTNAC | 2 cut(s) | 161, 401 |
| MalI | GATC | 7 cut(s) | 38, 98, 137, 211, 408, 445, 527 |
| MboI | GATC | 7 cut(s) | 36, 96, 135, 209, 406, 443, 525 |
| MboII | GAAGA | 6 cut(s) | 187, 213, 276, 413, 471, 545 |
| MflI | RGATCY | 1 cut(s) | 36 |
| MhlI | GDGCHC | 3 cut(s) | 172, 356, 610 |
| MluCI | AATT | 3 cut(s) | 28, 46, 250 |
| MlyI | GAGTC | 2 cut(s) | 98, 238 |
| MmeI | TCCRAC | 1 cut(s) | 187 |
| MnlI | CCTC | 4 cut(s) | 143, 389, 532, 567 |
| MseI | TTAA | 3 cut(s) | 27, 45, 302 |
| MspI | CCGG | 2 cut(s) | 324, 506 |
| MspR9I | CCNGG | 2 cut(s) | 325, 506 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 347 |
| NciI | CCSGG | 2 cut(s) | 325, 506 |
| NdeII | GATC | 7 cut(s) | 36, 96, 135, 209, 406, 443, 525 |
| NlaIII | CATG | 2 cut(s) | 263, 503 |
| NlaIV | GGNNCC | 1 cut(s) | 328 |
| NmeAIII | GCCGAG | 1 cut(s) | 291 |
| NmuCI | GTSAC | 1 cut(s) | 161 |
| NspI | RCATGY | 1 cut(s) | 503 |
| PaeI | GCATGC | 1 cut(s) | 503 |
| PciSI | GCTCTTC | 2 cut(s) | 426, 454 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 406 |
| PkrI | GCNGC | 2 cut(s) | 91, 155 |
| Ple19I | CGATCG | 1 cut(s) | 409 |
| PleI | GAGTC | 2 cut(s) | 98, 238 |
| PpsI | GAGTC | 2 cut(s) | 98, 238 |
| PpuMI | RGGWCCY | 1 cut(s) | 327 |
| PshBI | ATTAAT | 1 cut(s) | 45 |
| Psp124BI | GAGCTC | 2 cut(s) | 172, 356 |
| Psp5II | RGGWCCY | 1 cut(s) | 327 |
| PspEI | GGTNACC | 1 cut(s) | 161 |
| PspN4I | GGNNCC | 1 cut(s) | 328 |
| PspPI | GGNCC | 1 cut(s) | 327 |
| PspPPI | RGGWCCY | 1 cut(s) | 327 |
| PsuI | RGATCY | 1 cut(s) | 36 |
| PvuI | CGATCG | 1 cut(s) | 409 |
| RsaI | GTAC | 2 cut(s) | 435, 471 |
| RsaNI | GTAC | 2 cut(s) | 434, 470 |
| SacI | GAGCTC | 2 cut(s) | 172, 356 |
| SapI | GCTCTTC | 2 cut(s) | 426, 454 |
| SaqAI | TTAA | 3 cut(s) | 27, 45, 302 |
| SatI | GCNGC | 2 cut(s) | 90, 154 |
| Sau3AI | GATC | 7 cut(s) | 36, 96, 135, 209, 406, 443, 525 |
| Sau96I | GGNCC | 1 cut(s) | 327 |
| SchI | GAGTC | 2 cut(s) | 98, 238 |
| ScrFI | CCNGG | 2 cut(s) | 325, 506 |
| SduI | GDGCHC | 3 cut(s) | 172, 356, 610 |
| SfaNI | GCATC | 1 cut(s) | 61 |
| SinI | GGWCC | 1 cut(s) | 327 |
| SmlI | CTYRAG | 1 cut(s) | 364 |
| SmoI | CTYRAG | 1 cut(s) | 364 |
| SphI | GCATGC | 1 cut(s) | 503 |
| Sse9I | AATT | 3 cut(s) | 28, 46, 250 |
| SsiI | CCGC | 1 cut(s) | 154 |
| SspMI | CTAG | 1 cut(s) | 372 |
| SstI | GAGCTC | 2 cut(s) | 172, 356 |
| StyD4I | CCNGG | 2 cut(s) | 323, 504 |
| TaiI | ACGT | 3 cut(s) | 79, 295, 439 |
| TaqI | TCGA | 2 cut(s) | 409, 524 |
| TaqII | GACCGA | 1 cut(s) | 180 |
| TasI | AATT | 3 cut(s) | 28, 46, 250 |
| TatI | WGTACW | 1 cut(s) | 469 |
| TauI | GCSGC | 1 cut(s) | 156 |
| Tru1I | TTAA | 3 cut(s) | 27, 45, 302 |
| Tru9I | TTAA | 3 cut(s) | 27, 45, 302 |
| TscAI | CASTG | 1 cut(s) | 123 |
| TseFI | GTSAC | 1 cut(s) | 161 |
| TseI | GCWGC | 1 cut(s) | 89 |
| Tsp45I | GTSAC | 1 cut(s) | 161 |
| TspDTI | ATGAA | 2 cut(s) | 216, 333 |
| TspRI | CASTG | 1 cut(s) | 123 |
| VpaK11BI | GGWCC | 1 cut(s) | 327 |
| VspI | ATTAAT | 1 cut(s) | 45 |
| XceI | RCATGY | 1 cut(s) | 503 |
| XmiI | GTMKAC | 1 cut(s) | 79 |
| XspI | CTAG | 1 cut(s) | 372 |