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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 244 |
| Acc36I | ACCTGC | 1 cut(s) | 301 |
| AccII | CGCG | 4 cut(s) | 32, 242, 264, 606 |
| AciI | CCGC | 9 cut(s) | 30, 108, 240, 262, 363, 404, 423, 445, 647 |
| AclWI | GGATC | 2 cut(s) | 172, 530 |
| AcuI | CTGAAG | 1 cut(s) | 492 |
| AdeI | CACNNNGTG | 1 cut(s) | 14 |
| AgsI | TTSAA | 2 cut(s) | 337, 499 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 39 |
| AjnI | CCWGG | 2 cut(s) | 302, 572 |
| AleI | CACNNNNGTG | 1 cut(s) | 101 |
| AluBI | AGCT | 2 cut(s) | 347, 641 |
| AluI | AGCT | 2 cut(s) | 347, 641 |
| Alw26I | GTCTC | 2 cut(s) | 161, 230 |
| AlwI | GGATC | 2 cut(s) | 172, 530 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 644 |
| AoxI | GGCC | 4 cut(s) | 63, 456, 525, 612 |
| ApeKI | GCWGC | 4 cut(s) | 274, 307, 359, 650 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 361, 393 |
| AspLEI | GCGC | 3 cut(s) | 332, 343, 606 |
| AspS9I | GGNCC | 2 cut(s) | 406, 456 |
| AsuC2I | CCSGG | 1 cut(s) | 146 |
| AsuHPI | GGTGA | 3 cut(s) | 26, 122, 526 |
| AsuII | TTCGAA | 1 cut(s) | 134 |
| AvaII | GGWCC | 1 cut(s) | 406 |
| AxyI | CCTNAGG | 1 cut(s) | 529 |
| BbvI | GCAGC | 4 cut(s) | 286, 319, 346, 637 |
| BccI | CCATC | 5 cut(s) | 208, 362, 395, 446, 667 |
| BceAI | ACGGC | 1 cut(s) | 553 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 155, 189 |
| BciT130I | CCWGG | 2 cut(s) | 304, 574 |
| BcnI | CCSGG | 1 cut(s) | 146 |
| BcoDI | GTCTC | 2 cut(s) | 161, 230 |
| BfmI | CTRYAG | 1 cut(s) | 99 |
| BfoI | RGCGCY | 1 cut(s) | 333 |
| BfuAI | ACCTGC | 1 cut(s) | 301 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 338, 464 |
| BglII | AGATCT | 2 cut(s) | 223, 439 |
| BisI | GCNGC | 6 cut(s) | 275, 308, 360, 363, 648, 651 |
| BlsI | GCNGC | 6 cut(s) | 276, 309, 361, 364, 649, 652 |
| Bme1390I | CCNGG | 3 cut(s) | 146, 304, 574 |
| Bme18I | GGWCC | 1 cut(s) | 406 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 39 |
| BmgT120I | GGNCC | 2 cut(s) | 406, 456 |
| BmiI | GGNNCC | 2 cut(s) | 257, 457 |
| BmrFI | CCNGG | 3 cut(s) | 146, 304, 574 |
| BmsI | GCATC | 3 cut(s) | 70, 434, 507 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 8, 40 |
| Bpu14I | TTCGAA | 1 cut(s) | 134 |
| BpuMI | CCSGG | 1 cut(s) | 146 |
| BsaI | GGTCTC | 1 cut(s) | 230 |
| BsaJI | CCNNGG | 1 cut(s) | 550 |
| Bse21I | CCTNAGG | 1 cut(s) | 529 |
| Bse3DI | GCAATG | 1 cut(s) | 202 |
| BseBI | CCWGG | 2 cut(s) | 304, 574 |
| BseDI | CCNNGG | 1 cut(s) | 550 |
| BseGI | GGATG | 2 cut(s) | 387, 457 |
| BseMI | GCAATG | 1 cut(s) | 202 |
| BseMII | CTCAG | 1 cut(s) | 27 |
| BseRI | GAGGAG | 1 cut(s) | 248 |
| BseXI | GCAGC | 4 cut(s) | 286, 319, 346, 637 |
| BseYI | CCCAGC | 1 cut(s) | 564 |
| Bsh1236I | CGCG | 4 cut(s) | 32, 242, 264, 606 |
| Bsh1285I | CGRYCG | 1 cut(s) | 30 |
| BshFI | GGCC | 4 cut(s) | 65, 458, 527, 614 |
| BsiEI | CGRYCG | 1 cut(s) | 30 |
| BsiSI | CCGG | 1 cut(s) | 146 |
| BsmAI | GTCTC | 2 cut(s) | 161, 230 |
| BsmI | GAATGC | 2 cut(s) | 400, 520 |
| BsnI | GGCC | 4 cut(s) | 65, 458, 527, 614 |
| Bso31I | GGTCTC | 1 cut(s) | 230 |
| Bsp119I | TTCGAA | 1 cut(s) | 134 |
| Bsp143I | GATC | 4 cut(s) | 177, 223, 439, 535 |
| BspACI | CCGC | 9 cut(s) | 30, 108, 240, 262, 363, 404, 423, 445, 647 |
| BspANI | GGCC | 4 cut(s) | 65, 458, 527, 614 |
| BspCNI | CTCAG | 1 cut(s) | 28 |
| BspFNI | CGCG | 4 cut(s) | 32, 242, 264, 606 |
| BspLI | GGNNCC | 2 cut(s) | 257, 457 |
| BspMI | ACCTGC | 1 cut(s) | 301 |
| BspPI | GGATC | 2 cut(s) | 172, 530 |
| BspT104I | TTCGAA | 1 cut(s) | 134 |
| BspTNI | GGTCTC | 1 cut(s) | 230 |
| BsrDI | GCAATG | 1 cut(s) | 202 |
| BssECI | CCNNGG | 1 cut(s) | 550 |
| BssMI | GATC | 4 cut(s) | 177, 223, 439, 535 |
| BssT1I | CCWWGG | 1 cut(s) | 550 |
| Bst2UI | CCWGG | 2 cut(s) | 304, 574 |
| Bst4CI | ACNGT | 2 cut(s) | 91, 100 |
| Bst6I | CTCTTC | 1 cut(s) | 114 |
| BstBI | TTCGAA | 1 cut(s) | 134 |
| BstC8I | GCNNGC | 3 cut(s) | 67, 71, 345 |
| BstDEI | CTNAG | 2 cut(s) | 36, 529 |
| BstF5I | GGATG | 2 cut(s) | 387, 457 |
| BstFNI | CGCG | 4 cut(s) | 32, 242, 264, 606 |
| BstH2I | RGCGCY | 1 cut(s) | 333 |
| BstHHI | GCGC | 3 cut(s) | 332, 343, 606 |
| BstKTI | GATC | 4 cut(s) | 180, 226, 442, 538 |
| BstMAI | GTCTC | 2 cut(s) | 161, 230 |
| BstMBI | GATC | 4 cut(s) | 177, 223, 439, 535 |
| BstMCI | CGRYCG | 1 cut(s) | 30 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 227, 338, 464, 647 |
| BstNI | CCWGG | 2 cut(s) | 304, 574 |
| BstSCI | CCNGG | 3 cut(s) | 144, 302, 572 |
| BstSFI | CTRYAG | 1 cut(s) | 99 |
| BstUI | CGCG | 4 cut(s) | 32, 242, 264, 606 |
| BstV1I | GCAGC | 4 cut(s) | 286, 319, 346, 637 |
| BstX2I | RGATCY | 2 cut(s) | 223, 439 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 626 |
| BstYI | RGATCY | 2 cut(s) | 223, 439 |
| Bsu36I | CCTNAGG | 1 cut(s) | 529 |
| BsuRI | GGCC | 4 cut(s) | 65, 458, 527, 614 |
| BtgZI | GCGATG | 1 cut(s) | 340 |
| BtsCI | GGATG | 2 cut(s) | 387, 457 |
| BtsIMutI | CAGTG | 2 cut(s) | 87, 96 |
| BveI | ACCTGC | 1 cut(s) | 301 |
| Cac8I | GCNNGC | 3 cut(s) | 67, 71, 345 |
| CaiI | CAGNNNCTG | 1 cut(s) | 644 |
| CfoI | GCGC | 3 cut(s) | 332, 343, 606 |
| Cfr13I | GGNCC | 2 cut(s) | 406, 456 |
| CseI | GACGC | 3 cut(s) | 37, 227, 365 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 456, 491 |
| CviAII | CATG | 1 cut(s) | 503 |
| DdeI | CTNAG | 2 cut(s) | 36, 529 |
| DpnI | GATC | 4 cut(s) | 179, 225, 441, 537 |
| DpnII | GATC | 4 cut(s) | 177, 223, 439, 535 |
| DraIII | CACNNNGTG | 1 cut(s) | 14 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 244 |
| DriI | GACNNNNNGTC | 1 cut(s) | 39 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 244 |
| Eam1104I | CTCTTC | 1 cut(s) | 114 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 39 |
| EarI | CTCTTC | 1 cut(s) | 114 |
| Eco130I | CCWWGG | 1 cut(s) | 550 |
| Eco31I | GGTCTC | 1 cut(s) | 230 |
| Eco47I | GGWCC | 1 cut(s) | 406 |
| Eco57I | CTGAAG | 1 cut(s) | 492 |
| Eco81I | CCTNAGG | 1 cut(s) | 529 |
| EcoRII | CCWGG | 2 cut(s) | 302, 572 |
| EcoT14I | CCWWGG | 1 cut(s) | 550 |
| EcoT22I | ATGCAT | 1 cut(s) | 522 |
| ErhI | CCWWGG | 1 cut(s) | 550 |
| FaeI | CATG | 1 cut(s) | 506 |
| FatI | CATG | 1 cut(s) | 502 |
| Fnu4HI | GCNGC | 6 cut(s) | 275, 308, 360, 363, 648, 651 |
| FokI | GGATG | 2 cut(s) | 374, 464 |
| Fsp4HI | GCNGC | 6 cut(s) | 275, 308, 360, 363, 648, 651 |
| GlaI | GCGC | 3 cut(s) | 331, 342, 605 |
| GluI | GCNGC | 6 cut(s) | 275, 308, 360, 363, 648, 651 |
| GsaI | CCCAGC | 1 cut(s) | 568 |
| HaeII | RGCGCY | 1 cut(s) | 333 |
| HaeIII | GGCC | 4 cut(s) | 65, 458, 527, 614 |
| HapII | CCGG | 1 cut(s) | 146 |
| HgaI | GACGC | 3 cut(s) | 37, 227, 365 |
| HhaI | GCGC | 3 cut(s) | 332, 343, 606 |
| Hin1II | CATG | 1 cut(s) | 506 |
| Hin6I | GCGC | 3 cut(s) | 330, 341, 604 |
| HinP1I | GCGC | 3 cut(s) | 330, 341, 604 |
| HincII | GTYRAC | 1 cut(s) | 94 |
| HindII | GTYRAC | 1 cut(s) | 94 |
| HinfI | GANTC | 3 cut(s) | 19, 199, 283 |
| HpaII | CCGG | 1 cut(s) | 146 |
| HphI | GGTGA | 3 cut(s) | 26, 122, 526 |
| Hpy166II | GTNNAC | 1 cut(s) | 94 |
| Hpy188I | TCNGA | 2 cut(s) | 45, 250 |
| Hpy188III | TCNNGA | 2 cut(s) | 196, 391 |
| Hpy8I | GTNNAC | 1 cut(s) | 94 |
| Hpy99I | CGWCG | 1 cut(s) | 197 |
| HpyAV | CCTTC | 2 cut(s) | 143, 331 |
| HpyCH4III | ACNGT | 2 cut(s) | 91, 100 |
| HpyCH4IV | ACGT | 1 cut(s) | 192 |
| HpyCH4V | TGCA | 4 cut(s) | 207, 293, 484, 520 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 227, 338, 464, 647 |
| HpyF3I | CTNAG | 2 cut(s) | 36, 529 |
| HpySE526I | ACGT | 1 cut(s) | 192 |
| Hsp92II | CATG | 1 cut(s) | 506 |
| HspAI | GCGC | 3 cut(s) | 330, 341, 604 |
| Kzo9I | GATC | 4 cut(s) | 177, 223, 439, 535 |
| LmnI | GCTCC | 2 cut(s) | 152, 261 |
| Lsp1109I | GCAGC | 4 cut(s) | 286, 319, 346, 637 |
| LweI | GCATC | 3 cut(s) | 70, 434, 507 |
| MaeII | ACGT | 1 cut(s) | 192 |
| MaeIII | GTNAC | 1 cut(s) | 313 |
| MalI | GATC | 4 cut(s) | 179, 225, 441, 537 |
| MboI | GATC | 4 cut(s) | 177, 223, 439, 535 |
| MboII | GAAGA | 3 cut(s) | 131, 223, 624 |
| MfeI | CAATTG | 1 cut(s) | 171 |
| MflI | RGATCY | 2 cut(s) | 223, 439 |
| MluCI | AATT | 2 cut(s) | 171, 494 |
| MlyI | GAGTC | 1 cut(s) | 277 |
| MnlI | CCTC | 6 cut(s) | 31, 94, 109, 115, 269, 505 |
| Mph1103I | ATGCAT | 1 cut(s) | 522 |
| MslI | CAYNNNNRTG | 1 cut(s) | 101 |
| MspI | CCGG | 1 cut(s) | 146 |
| MspR9I | CCNGG | 3 cut(s) | 146, 304, 574 |
| MunI | CAATTG | 1 cut(s) | 171 |
| Mva1269I | GAATGC | 2 cut(s) | 400, 520 |
| MvaI | CCWGG | 2 cut(s) | 304, 574 |
| MvnI | CGCG | 4 cut(s) | 32, 242, 264, 606 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 227, 338, 464, 647 |
| NciI | CCSGG | 1 cut(s) | 146 |
| NdeII | GATC | 4 cut(s) | 177, 223, 439, 535 |
| NlaIII | CATG | 1 cut(s) | 506 |
| NlaIV | GGNNCC | 2 cut(s) | 257, 457 |
| NmuCI | GTSAC | 1 cut(s) | 313 |
| NsiI | ATGCAT | 1 cut(s) | 522 |
| NspV | TTCGAA | 1 cut(s) | 134 |
| OliI | CACNNNNGTG | 1 cut(s) | 101 |
| PctI | GAATGC | 2 cut(s) | 400, 520 |
| PfeI | GAWTC | 2 cut(s) | 19, 199 |
| PfoI | TCCNGGA | 1 cut(s) | 144 |
| PkrI | GCNGC | 6 cut(s) | 276, 309, 361, 364, 649, 652 |
| PleI | GAGTC | 1 cut(s) | 277 |
| PpsI | GAGTC | 1 cut(s) | 277 |
| Psp6I | CCWGG | 2 cut(s) | 302, 572 |
| PspFI | CCCAGC | 1 cut(s) | 564 |
| PspGI | CCWGG | 2 cut(s) | 302, 572 |
| PspN4I | GGNNCC | 2 cut(s) | 257, 457 |
| PspPI | GGNCC | 2 cut(s) | 406, 456 |
| PstNI | CAGNNNCTG | 1 cut(s) | 644 |
| PsuI | RGATCY | 2 cut(s) | 223, 439 |
| RseI | CAYNNNNRTG | 1 cut(s) | 101 |
| SatI | GCNGC | 6 cut(s) | 275, 308, 360, 363, 648, 651 |
| Sau3AI | GATC | 4 cut(s) | 177, 223, 439, 535 |
| Sau96I | GGNCC | 2 cut(s) | 406, 456 |
| SchI | GAGTC | 1 cut(s) | 277 |
| ScrFI | CCNGG | 3 cut(s) | 146, 304, 574 |
| SetI | ASST | 8 cut(s) | 16, 161, 195, 315, 349, 516, 578, 643 |
| SfaNI | GCATC | 3 cut(s) | 70, 434, 507 |
| SfcI | CTRYAG | 1 cut(s) | 99 |
| SfuI | TTCGAA | 1 cut(s) | 134 |
| SinI | GGWCC | 1 cut(s) | 406 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 101 |
| Sse9I | AATT | 2 cut(s) | 171, 494 |
| SsiI | CCGC | 9 cut(s) | 30, 108, 240, 262, 363, 404, 423, 445, 647 |
| StyD4I | CCNGG | 3 cut(s) | 144, 302, 572 |
| StyI | CCWWGG | 1 cut(s) | 550 |
| TaaI | ACNGT | 2 cut(s) | 91, 100 |
| TaiI | ACGT | 1 cut(s) | 195 |
| TaqI | TCGA | 4 cut(s) | 26, 134, 195, 270 |
| TasI | AATT | 2 cut(s) | 171, 494 |
| TauI | GCSGC | 2 cut(s) | 365, 650 |
| TfiI | GAWTC | 2 cut(s) | 19, 199 |
| TscAI | CASTG | 2 cut(s) | 94, 103 |
| TseFI | GTSAC | 1 cut(s) | 313 |
| TseI | GCWGC | 4 cut(s) | 274, 307, 359, 650 |
| Tsp45I | GTSAC | 1 cut(s) | 313 |
| TspRI | CASTG | 2 cut(s) | 94, 103 |
| VpaK11BI | GGWCC | 1 cut(s) | 406 |
| Zsp2I | ATGCAT | 1 cut(s) | 522 |