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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 513 |
| AccI | GTMKAC | 1 cut(s) | 143 |
| AciI | CCGC | 7 cut(s) | 54, 230, 254, 305, 351, 422, 655 |
| AfaI | GTAC | 1 cut(s) | 461 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 92 |
| AgsI | TTSAA | 1 cut(s) | 432 |
| AhlI | ACTAGT | 2 cut(s) | 64, 161 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 131, 163 |
| AleI | CACNNNNGTG | 1 cut(s) | 95 |
| AluBI | AGCT | 5 cut(s) | 59, 340, 347, 514, 586 |
| AluI | AGCT | 5 cut(s) | 59, 340, 347, 514, 586 |
| Alw26I | GTCTC | 1 cut(s) | 282 |
| AoxI | GGCC | 2 cut(s) | 552, 565 |
| ApeKI | GCWGC | 4 cut(s) | 56, 59, 347, 583 |
| AspS9I | GGNCC | 3 cut(s) | 70, 275, 553 |
| AsuC2I | CCSGG | 1 cut(s) | 94 |
| AsuHPI | GGTGA | 2 cut(s) | 109, 294 |
| AvaII | GGWCC | 2 cut(s) | 70, 275 |
| AxyI | CCTNAGG | 1 cut(s) | 314 |
| BbvI | GCAGC | 4 cut(s) | 46, 68, 334, 595 |
| BccI | CCATC | 1 cut(s) | 613 |
| BcnI | CCSGG | 1 cut(s) | 94 |
| BcoDI | GTCTC | 1 cut(s) | 282 |
| BcuI | ACTAGT | 2 cut(s) | 64, 161 |
| BfaI | CTAG | 4 cut(s) | 18, 65, 162, 587 |
| BfmI | CTRYAG | 2 cut(s) | 483, 548 |
| BfuAI | ACCTGC | 1 cut(s) | 513 |
| BisI | GCNGC | 5 cut(s) | 57, 60, 348, 351, 584 |
| BlsI | GCNGC | 5 cut(s) | 58, 61, 349, 352, 585 |
| Bme1390I | CCNGG | 1 cut(s) | 94 |
| Bme18I | GGWCC | 2 cut(s) | 70, 275 |
| BmgT120I | GGNCC | 3 cut(s) | 70, 275, 553 |
| BmiI | GGNNCC | 3 cut(s) | 45, 277, 651 |
| BmrFI | CCNGG | 1 cut(s) | 94 |
| BmsI | GCATC | 1 cut(s) | 186 |
| BpmI | CTGGAG | 1 cut(s) | 30 |
| Bpu10I | CCTNAGC | 1 cut(s) | 600 |
| BpuMI | CCSGG | 1 cut(s) | 94 |
| BsaJI | CCNNGG | 1 cut(s) | 93 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 440, 470 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 92 |
| Bse1I | ACTGG | 2 cut(s) | 277, 633 |
| Bse21I | CCTNAGG | 1 cut(s) | 314 |
| BseDI | CCNNGG | 1 cut(s) | 93 |
| BseGI | GGATG | 3 cut(s) | 177, 376, 434 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 92 |
| BseMII | CTCAG | 1 cut(s) | 591 |
| BseNI | ACTGG | 2 cut(s) | 277, 633 |
| BseXI | GCAGC | 4 cut(s) | 46, 68, 334, 595 |
| BsgI | GTGCAG | 1 cut(s) | 45 |
| BshFI | GGCC | 2 cut(s) | 554, 567 |
| BsiSI | CCGG | 1 cut(s) | 93 |
| BslFI | GGGAC | 1 cut(s) | 261 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 92 |
| BsmAI | GTCTC | 1 cut(s) | 282 |
| BsmFI | GGGAC | 1 cut(s) | 261 |
| BsnI | GGCC | 2 cut(s) | 554, 567 |
| Bsp143I | GATC | 1 cut(s) | 671 |
| BspACI | CCGC | 7 cut(s) | 54, 230, 254, 305, 351, 422, 655 |
| BspANI | GGCC | 2 cut(s) | 554, 567 |
| BspCNI | CTCAG | 1 cut(s) | 592 |
| BspLI | GGNNCC | 3 cut(s) | 45, 277, 651 |
| BspMI | ACCTGC | 1 cut(s) | 513 |
| BspQI | GCTCTTC | 1 cut(s) | 371 |
| BsrI | ACTGG | 2 cut(s) | 277, 633 |
| BssECI | CCNNGG | 1 cut(s) | 93 |
| BssMI | GATC | 1 cut(s) | 671 |
| Bst6I | CTCTTC | 2 cut(s) | 189, 371 |
| BstC8I | GCNNGC | 1 cut(s) | 389 |
| BstDEI | CTNAG | 4 cut(s) | 12, 314, 501, 600 |
| BstF5I | GGATG | 3 cut(s) | 177, 376, 434 |
| BstKTI | GATC | 1 cut(s) | 674 |
| BstMAI | GTCTC | 1 cut(s) | 282 |
| BstMBI | GATC | 1 cut(s) | 671 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 56, 583 |
| BstSCI | CCNGG | 1 cut(s) | 92 |
| BstSFI | CTRYAG | 2 cut(s) | 483, 548 |
| BstV1I | GCAGC | 4 cut(s) | 46, 68, 334, 595 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 240, 272 |
| Bsu36I | CCTNAGG | 1 cut(s) | 314 |
| BsuRI | GGCC | 2 cut(s) | 554, 567 |
| BtsCI | GGATG | 3 cut(s) | 177, 376, 434 |
| BtsIMutI | CAGTG | 1 cut(s) | 626 |
| BveI | ACCTGC | 1 cut(s) | 513 |
| Cac8I | GCNNGC | 1 cut(s) | 389 |
| Cfr13I | GGNCC | 3 cut(s) | 70, 275, 553 |
| Csp6I | GTAC | 1 cut(s) | 460 |
| CviAII | CATG | 1 cut(s) | 103 |
| CviQI | GTAC | 1 cut(s) | 460 |
| DdeI | CTNAG | 4 cut(s) | 12, 314, 501, 600 |
| DpnI | GATC | 1 cut(s) | 673 |
| DpnII | GATC | 1 cut(s) | 671 |
| Eam1104I | CTCTTC | 2 cut(s) | 189, 371 |
| EarI | CTCTTC | 2 cut(s) | 189, 371 |
| EciI | GGCGGA | 1 cut(s) | 411 |
| Eco147I | AGGCCT | 1 cut(s) | 567 |
| Eco32I | GATATC | 2 cut(s) | 400, 490 |
| Eco47I | GGWCC | 2 cut(s) | 70, 275 |
| Eco81I | CCTNAGG | 1 cut(s) | 314 |
| EcoO109I | RGGNCCY | 1 cut(s) | 553 |
| EcoRV | GATATC | 2 cut(s) | 400, 490 |
| FaeI | CATG | 1 cut(s) | 106 |
| FaiI | YATR | 2 cut(s) | 104, 415 |
| FaqI | GGGAC | 1 cut(s) | 261 |
| FatI | CATG | 1 cut(s) | 102 |
| FauI | CCCGC | 2 cut(s) | 237, 662 |
| FblI | GTMKAC | 1 cut(s) | 143 |
| Fnu4HI | GCNGC | 5 cut(s) | 57, 60, 348, 351, 584 |
| FokI | GGATG | 3 cut(s) | 164, 383, 421 |
| Fsp4HI | GCNGC | 5 cut(s) | 57, 60, 348, 351, 584 |
| FspBI | CTAG | 4 cut(s) | 18, 65, 162, 587 |
| GluI | GCNGC | 5 cut(s) | 57, 60, 348, 351, 584 |
| GsuI | CTGGAG | 1 cut(s) | 30 |
| HaeIII | GGCC | 2 cut(s) | 554, 567 |
| HapII | CCGG | 1 cut(s) | 93 |
| Hin1II | CATG | 1 cut(s) | 106 |
| HinfI | GANTC | 3 cut(s) | 21, 299, 320 |
| HpaII | CCGG | 1 cut(s) | 93 |
| HphI | GGTGA | 2 cut(s) | 109, 294 |
| Hpy166II | GTNNAC | 4 cut(s) | 70, 100, 144, 462 |
| Hpy188III | TCNNGA | 4 cut(s) | 86, 334, 403, 664 |
| Hpy8I | GTNNAC | 4 cut(s) | 70, 100, 144, 462 |
| HpyAV | CCTTC | 3 cut(s) | 435, 507, 588 |
| HpyCH4V | TGCA | 1 cut(s) | 62 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 56, 583 |
| HpyF3I | CTNAG | 4 cut(s) | 12, 314, 501, 600 |
| Hsp92II | CATG | 1 cut(s) | 106 |
| Kzo9I | GATC | 1 cut(s) | 671 |
| LguI | GCTCTTC | 1 cut(s) | 371 |
| LmnI | GCTCC | 2 cut(s) | 49, 655 |
| Lsp1109I | GCAGC | 4 cut(s) | 46, 68, 334, 595 |
| LweI | GCATC | 1 cut(s) | 186 |
| MaeI | CTAG | 4 cut(s) | 18, 65, 162, 587 |
| MaeIII | GTNAC | 1 cut(s) | 112 |
| MalI | GATC | 1 cut(s) | 673 |
| MboI | GATC | 1 cut(s) | 671 |
| MboII | GAAGA | 3 cut(s) | 206, 388, 420 |
| MfeI | CAATTG | 1 cut(s) | 182 |
| MluCI | AATT | 1 cut(s) | 182 |
| MmeI | TCCRAC | 1 cut(s) | 289 |
| MnlI | CCTC | 6 cut(s) | 34, 190, 261, 442, 566, 616 |
| MseI | TTAA | 3 cut(s) | 32, 573, 591 |
| MslI | CAYNNNNRTG | 1 cut(s) | 95 |
| MspA1I | CMGCKG | 1 cut(s) | 59 |
| MspI | CCGG | 1 cut(s) | 93 |
| MspR9I | CCNGG | 1 cut(s) | 94 |
| MunI | CAATTG | 1 cut(s) | 182 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 56, 583 |
| NciI | CCSGG | 1 cut(s) | 94 |
| NdeII | GATC | 1 cut(s) | 671 |
| NlaIII | CATG | 1 cut(s) | 106 |
| NlaIV | GGNNCC | 3 cut(s) | 45, 277, 651 |
| NmuCI | GTSAC | 1 cut(s) | 112 |
| OliI | CACNNNNGTG | 1 cut(s) | 95 |
| PceI | AGGCCT | 1 cut(s) | 567 |
| PciSI | GCTCTTC | 1 cut(s) | 371 |
| PfeI | GAWTC | 3 cut(s) | 21, 299, 320 |
| PkrI | GCNGC | 5 cut(s) | 58, 61, 349, 352, 585 |
| PspN4I | GGNNCC | 3 cut(s) | 45, 277, 651 |
| PspPI | GGNCC | 3 cut(s) | 70, 275, 553 |
| PvuII | CAGCTG | 1 cut(s) | 59 |
| RsaI | GTAC | 1 cut(s) | 461 |
| RsaNI | GTAC | 1 cut(s) | 460 |
| RseI | CAYNNNNRTG | 1 cut(s) | 95 |
| SapI | GCTCTTC | 1 cut(s) | 371 |
| SaqAI | TTAA | 3 cut(s) | 32, 573, 591 |
| SatI | GCNGC | 5 cut(s) | 57, 60, 348, 351, 584 |
| Sau3AI | GATC | 1 cut(s) | 671 |
| Sau96I | GGNCC | 3 cut(s) | 70, 275, 553 |
| ScrFI | CCNGG | 1 cut(s) | 94 |
| SetI | ASST | 9 cut(s) | 61, 75, 201, 342, 349, 516, 527, 588, 679 |
| SfaNI | GCATC | 1 cut(s) | 186 |
| SfcI | CTRYAG | 2 cut(s) | 483, 548 |
| SinI | GGWCC | 2 cut(s) | 70, 275 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 95 |
| SpeI | ACTAGT | 2 cut(s) | 64, 161 |
| Sse9I | AATT | 1 cut(s) | 182 |
| SseBI | AGGCCT | 1 cut(s) | 567 |
| SsiI | CCGC | 7 cut(s) | 54, 230, 254, 305, 351, 422, 655 |
| SspMI | CTAG | 4 cut(s) | 18, 65, 162, 587 |
| StuI | AGGCCT | 1 cut(s) | 567 |
| StyD4I | CCNGG | 1 cut(s) | 92 |
| TasI | AATT | 1 cut(s) | 182 |
| TauI | GCSGC | 1 cut(s) | 353 |
| TfiI | GAWTC | 3 cut(s) | 21, 299, 320 |
| Tru1I | TTAA | 3 cut(s) | 32, 573, 591 |
| Tru9I | TTAA | 3 cut(s) | 32, 573, 591 |
| TscAI | CASTG | 1 cut(s) | 633 |
| TseFI | GTSAC | 1 cut(s) | 112 |
| TseI | GCWGC | 4 cut(s) | 56, 59, 347, 583 |
| Tsp45I | GTSAC | 1 cut(s) | 112 |
| TspDTI | ATGAA | 1 cut(s) | 372 |
| TspRI | CASTG | 1 cut(s) | 633 |
| VpaK11BI | GGWCC | 2 cut(s) | 70, 275 |
| XmiI | GTMKAC | 1 cut(s) | 143 |
| XspI | CTAG | 4 cut(s) | 18, 65, 162, 587 |