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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 294 |
| Acc36I | ACCTGC | 1 cut(s) | 294 |
| AccB1I | GGYRCC | 1 cut(s) | 949 |
| AccBSI | CCGCTC | 1 cut(s) | 134 |
| AccII | CGCG | 5 cut(s) | 209, 465, 638, 845, 1052 |
| AclWI | GGATC | 1 cut(s) | 955 |
| AcoI | YGGCCR | 1 cut(s) | 846 |
| AcsI | RAATTY | 1 cut(s) | 718 |
| AcuI | CTGAAG | 3 cut(s) | 495, 582, 789 |
| AcyI | GRCGYC | 1 cut(s) | 950 |
| AfaI | GTAC | 3 cut(s) | 15, 205, 249 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 760, 869 |
| AgeI | ACCGGT | 1 cut(s) | 98 |
| AgsI | TTSAA | 1 cut(s) | 713 |
| AjnI | CCWGG | 1 cut(s) | 621 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 471, 503 |
| AluBI | AGCT | 4 cut(s) | 361, 629, 697, 941 |
| AluI | AGCT | 4 cut(s) | 361, 629, 697, 941 |
| Alw26I | GTCTC | 1 cut(s) | 187 |
| AlwI | GGATC | 1 cut(s) | 955 |
| AoxI | GGCC | 3 cut(s) | 61, 846, 1047 |
| ApeKI | GCWGC | 3 cut(s) | 358, 697, 866 |
| ApoI | RAATTY | 1 cut(s) | 718 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 145, 177 |
| AsiGI | ACCGGT | 1 cut(s) | 98 |
| AspLEI | GCGC | 2 cut(s) | 638, 952 |
| AspS9I | GGNCC | 4 cut(s) | 175, 328, 763, 1047 |
| AsuHPI | GGTGA | 5 cut(s) | 113, 578, 610, 832, 980 |
| AsuNHI | GCTAGC | 1 cut(s) | 410 |
| AvaII | GGWCC | 3 cut(s) | 175, 328, 763 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 187, 220, 969, 1002 |
| BanI | GGYRCC | 1 cut(s) | 949 |
| BbvI | GCAGC | 3 cut(s) | 370, 684, 878 |
| BccI | CCATC | 2 cut(s) | 520, 736 |
| BceAI | ACGGC | 1 cut(s) | 788 |
| BciT130I | CCWGG | 1 cut(s) | 623 |
| BclI | TGATCA | 1 cut(s) | 1041 |
| BcoDI | GTCTC | 1 cut(s) | 187 |
| BfaI | CTAG | 6 cut(s) | 179, 230, 411, 612, 689, 1057 |
| BfmI | CTRYAG | 1 cut(s) | 199 |
| BfoI | RGCGCY | 1 cut(s) | 953 |
| BfuAI | ACCTGC | 1 cut(s) | 294 |
| BisI | GCNGC | 6 cut(s) | 359, 582, 698, 846, 867, 1050 |
| BlsI | GCNGC | 6 cut(s) | 360, 583, 699, 847, 868, 1051 |
| BmcAI | AGTACT | 2 cut(s) | 15, 249 |
| Bme1390I | CCNGG | 1 cut(s) | 623 |
| Bme18I | GGWCC | 3 cut(s) | 175, 328, 763 |
| BmgT120I | GGNCC | 4 cut(s) | 175, 328, 763, 1047 |
| BmiI | GGNNCC | 5 cut(s) | 6, 193, 302, 329, 951 |
| BmrFI | CCNGG | 1 cut(s) | 623 |
| BmrI | ACTGGG | 1 cut(s) | 316 |
| BmsI | GCATC | 2 cut(s) | 595, 624 |
| BmtI | GCTAGC | 1 cut(s) | 414 |
| BmuI | ACTGGG | 1 cut(s) | 316 |
| BpmI | CTGGAG | 4 cut(s) | 56, 96, 319, 415 |
| BsaHI | GRCGYC | 1 cut(s) | 950 |
| BsaJI | CCNNGG | 4 cut(s) | 186, 621, 843, 910 |
| BsaWI | WCCGGW | 2 cut(s) | 98, 963 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 906, 936 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 760, 869 |
| Bse118I | RCCGGY | 1 cut(s) | 98 |
| Bse1I | ACTGG | 5 cut(s) | 113, 302, 322, 432, 486 |
| Bse3DI | GCAATG | 1 cut(s) | 1044 |
| BseBI | CCWGG | 1 cut(s) | 623 |
| BseDI | CCNNGG | 4 cut(s) | 186, 621, 843, 910 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 760, 869 |
| BseMI | GCAATG | 1 cut(s) | 1044 |
| BseMII | CTCAG | 1 cut(s) | 522 |
| BseNI | ACTGG | 5 cut(s) | 113, 302, 322, 432, 486 |
| BseRI | GAGGAG | 2 cut(s) | 987, 1040 |
| BseXI | GCAGC | 3 cut(s) | 370, 684, 878 |
| BseYI | CCCAGC | 1 cut(s) | 585 |
| Bsh1236I | CGCG | 5 cut(s) | 209, 465, 638, 845, 1052 |
| BshFI | GGCC | 3 cut(s) | 63, 848, 1049 |
| BshNI | GGYRCC | 1 cut(s) | 949 |
| BshTI | ACCGGT | 1 cut(s) | 98 |
| BsiSI | CCGG | 2 cut(s) | 99, 964 |
| BslFI | GGGAC | 2 cut(s) | 101, 341 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 760, 869 |
| BsmAI | GTCTC | 1 cut(s) | 187 |
| BsmFI | GGGAC | 2 cut(s) | 101, 341 |
| BsmI | GAATGC | 1 cut(s) | 685 |
| BsnI | GGCC | 3 cut(s) | 63, 848, 1049 |
| Bsp143I | GATC | 3 cut(s) | 960, 1003, 1041 |
| Bsp19I | CCATGG | 1 cut(s) | 910 |
| BspANI | GGCC | 3 cut(s) | 63, 848, 1049 |
| BspCNI | CTCAG | 1 cut(s) | 523 |
| BspFNI | CGCG | 5 cut(s) | 209, 465, 638, 845, 1052 |
| BspHI | TCATGA | 1 cut(s) | 34 |
| BspLI | GGNNCC | 5 cut(s) | 6, 193, 302, 329, 951 |
| BspMI | ACCTGC | 1 cut(s) | 294 |
| BspOI | GCTAGC | 1 cut(s) | 414 |
| BspPI | GGATC | 1 cut(s) | 955 |
| BspQI | GCTCTTC | 1 cut(s) | 687 |
| BspT107I | GGYRCC | 1 cut(s) | 949 |
| BsrBI | CCGCTC | 1 cut(s) | 134 |
| BsrDI | GCAATG | 1 cut(s) | 1044 |
| BsrFI | RCCGGY | 1 cut(s) | 98 |
| BsrI | ACTGG | 5 cut(s) | 113, 302, 322, 432, 486 |
| BssAI | RCCGGY | 1 cut(s) | 98 |
| BssECI | CCNNGG | 4 cut(s) | 186, 621, 843, 910 |
| BssMI | GATC | 3 cut(s) | 960, 1003, 1041 |
| BssNI | GRCGYC | 1 cut(s) | 950 |
| BssT1I | CCWWGG | 2 cut(s) | 186, 910 |
| Bst2UI | CCWGG | 1 cut(s) | 623 |
| Bst4CI | ACNGT | 6 cut(s) | 200, 220, 247, 252, 461, 982 |
| Bst6I | CTCTTC | 1 cut(s) | 687 |
| BstACI | GRCGYC | 1 cut(s) | 950 |
| BstC8I | GCNNGC | 5 cut(s) | 65, 318, 412, 453, 864 |
| BstDEI | CTNAG | 2 cut(s) | 531, 1007 |
| BstDSI | CCRYGG | 2 cut(s) | 843, 910 |
| BstEII | GGTNACC | 2 cut(s) | 101, 838 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 758 |
| BstFNI | CGCG | 5 cut(s) | 209, 465, 638, 845, 1052 |
| BstH2I | RGCGCY | 1 cut(s) | 953 |
| BstHHI | GCGC | 2 cut(s) | 638, 952 |
| BstKTI | GATC | 3 cut(s) | 963, 1006, 1044 |
| BstMAI | GTCTC | 1 cut(s) | 187 |
| BstMBI | GATC | 3 cut(s) | 960, 1003, 1041 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 358, 635, 947 |
| BstNI | CCWGG | 1 cut(s) | 623 |
| BstNSI | RCATGY | 2 cut(s) | 320, 455 |
| BstPI | GGTNACC | 2 cut(s) | 101, 838 |
| BstSCI | CCNGG | 1 cut(s) | 621 |
| BstSFI | CTRYAG | 1 cut(s) | 199 |
| BstUI | CGCG | 5 cut(s) | 209, 465, 638, 845, 1052 |
| BstV1I | GCAGC | 3 cut(s) | 370, 684, 878 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 821, 917 |
| BsuRI | GGCC | 3 cut(s) | 63, 848, 1049 |
| BtgI | CCRYGG | 2 cut(s) | 843, 910 |
| BtgZI | GCGATG | 1 cut(s) | 618 |
| BtsIMutI | CAGTG | 5 cut(s) | 149, 329, 813, 956, 987 |
| BveI | ACCTGC | 1 cut(s) | 294 |
| Cac8I | GCNNGC | 5 cut(s) | 65, 318, 412, 453, 864 |
| CciI | TCATGA | 1 cut(s) | 34 |
| CfoI | GCGC | 2 cut(s) | 638, 952 |
| Cfr10I | RCCGGY | 1 cut(s) | 98 |
| Cfr13I | GGNCC | 4 cut(s) | 175, 328, 763, 1047 |
| Cfr42I | CCGCGG | 1 cut(s) | 846 |
| CseI | GACGC | 1 cut(s) | 597 |
| Csp6I | GTAC | 3 cut(s) | 14, 204, 248 |
| CspAI | ACCGGT | 1 cut(s) | 98 |
| CviAII | CATG | 5 cut(s) | 35, 317, 452, 734, 911 |
| CviQI | GTAC | 3 cut(s) | 14, 204, 248 |
| DdeI | CTNAG | 2 cut(s) | 531, 1007 |
| DinI | GGCGCC | 1 cut(s) | 951 |
| DpnI | GATC | 3 cut(s) | 962, 1005, 1043 |
| DpnII | GATC | 3 cut(s) | 960, 1003, 1041 |
| EaeI | YGGCCR | 1 cut(s) | 846 |
| Eam1104I | CTCTTC | 1 cut(s) | 687 |
| EarI | CTCTTC | 1 cut(s) | 687 |
| EciI | GGCGGA | 1 cut(s) | 498 |
| Eco130I | CCWWGG | 2 cut(s) | 186, 910 |
| Eco47I | GGWCC | 3 cut(s) | 175, 328, 763 |
| Eco57I | CTGAAG | 3 cut(s) | 495, 582, 789 |
| Eco91I | GGTNACC | 2 cut(s) | 101, 838 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 758 |
| EcoO109I | RGGNCCY | 1 cut(s) | 328 |
| EcoO65I | GGTNACC | 2 cut(s) | 101, 838 |
| EcoRII | CCWGG | 1 cut(s) | 621 |
| EcoT14I | CCWWGG | 2 cut(s) | 186, 910 |
| EgeI | GGCGCC | 1 cut(s) | 951 |
| EheI | GGCGCC | 1 cut(s) | 951 |
| ErhI | CCWWGG | 2 cut(s) | 186, 910 |
| FaeI | CATG | 5 cut(s) | 38, 320, 455, 737, 914 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 684, 716 |
| FaqI | GGGAC | 2 cut(s) | 101, 341 |
| FatI | CATG | 5 cut(s) | 34, 316, 451, 733, 910 |
| FauI | CCCGC | 1 cut(s) | 862 |
| FbaI | TGATCA | 1 cut(s) | 1041 |
| Fnu4HI | GCNGC | 6 cut(s) | 359, 582, 698, 846, 867, 1050 |
| Fsp4HI | GCNGC | 6 cut(s) | 359, 582, 698, 846, 867, 1050 |
| FspBI | CTAG | 6 cut(s) | 179, 230, 411, 612, 689, 1057 |
| GlaI | GCGC | 2 cut(s) | 637, 951 |
| GluI | GCNGC | 6 cut(s) | 359, 582, 698, 846, 867, 1050 |
| GsaI | CCCAGC | 1 cut(s) | 589 |
| GsuI | CTGGAG | 4 cut(s) | 56, 96, 319, 415 |
| HaeII | RGCGCY | 1 cut(s) | 953 |
| HaeIII | GGCC | 3 cut(s) | 63, 848, 1049 |
| HapII | CCGG | 2 cut(s) | 99, 964 |
| HgaI | GACGC | 1 cut(s) | 597 |
| HhaI | GCGC | 2 cut(s) | 638, 952 |
| Hin1I | GRCGYC | 1 cut(s) | 950 |
| Hin1II | CATG | 5 cut(s) | 38, 320, 455, 737, 914 |
| Hin6I | GCGC | 2 cut(s) | 636, 950 |
| HinP1I | GCGC | 2 cut(s) | 636, 950 |
| HincII | GTYRAC | 2 cut(s) | 280, 405 |
| HindII | GTYRAC | 2 cut(s) | 280, 405 |
| HindIII | AAGCTT | 1 cut(s) | 939 |
| HinfI | GANTC | 2 cut(s) | 122, 596 |
| HpaI | GTTAAC | 1 cut(s) | 405 |
| HpaII | CCGG | 2 cut(s) | 99, 964 |
| HphI | GGTGA | 5 cut(s) | 113, 578, 610, 832, 980 |
| Hpy166II | GTNNAC | 5 cut(s) | 280, 399, 405, 786, 988 |
| Hpy188I | TCNGA | 1 cut(s) | 475 |
| Hpy188III | TCNNGA | 6 cut(s) | 35, 73, 490, 674, 793, 1001 |
| Hpy8I | GTNNAC | 5 cut(s) | 280, 399, 405, 786, 988 |
| Hpy99I | CGWCG | 2 cut(s) | 774, 883 |
| HpyAV | CCTTC | 5 cut(s) | 99, 557, 719, 813, 829 |
| HpyCH4III | ACNGT | 6 cut(s) | 200, 220, 247, 252, 461, 982 |
| HpyCH4IV | ACGT | 2 cut(s) | 401, 878 |
| HpyCH4V | TGCA | 7 cut(s) | 264, 289, 337, 352, 379, 455, 758 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 358, 635, 947 |
| HpyF3I | CTNAG | 2 cut(s) | 531, 1007 |
| HpySE526I | ACGT | 2 cut(s) | 401, 878 |
| Hsp92I | GRCGYC | 1 cut(s) | 950 |
| Hsp92II | CATG | 5 cut(s) | 38, 320, 455, 737, 914 |
| HspAI | GCGC | 2 cut(s) | 636, 950 |
| KasI | GGCGCC | 1 cut(s) | 949 |
| Ksp22I | TGATCA | 1 cut(s) | 1041 |
| KspAI | GTTAAC | 1 cut(s) | 405 |
| KspI | CCGCGG | 1 cut(s) | 846 |
| Kzo9I | GATC | 3 cut(s) | 960, 1003, 1041 |
| LguI | GCTCTTC | 1 cut(s) | 687 |
| LmnI | GCTCC | 2 cut(s) | 191, 300 |
| Lsp1109I | GCAGC | 3 cut(s) | 370, 684, 878 |
| LweI | GCATC | 2 cut(s) | 595, 624 |
| MaeI | CTAG | 6 cut(s) | 179, 230, 411, 612, 689, 1057 |
| MaeII | ACGT | 2 cut(s) | 401, 878 |
| MaeIII | GTNAC | 5 cut(s) | 101, 252, 434, 640, 838 |
| MalI | GATC | 3 cut(s) | 962, 1005, 1043 |
| MbiI | CCGCTC | 1 cut(s) | 134 |
| MboI | GATC | 3 cut(s) | 960, 1003, 1041 |
| MboII | GAAGA | 3 cut(s) | 488, 704, 751 |
| MluCI | AATT | 1 cut(s) | 718 |
| Mly113I | GGCGCC | 1 cut(s) | 950 |
| MlyI | GAGTC | 1 cut(s) | 605 |
| MmeI | TCCRAC | 1 cut(s) | 802 |
| MseI | TTAA | 5 cut(s) | 18, 45, 404, 702, 933 |
| MslI | CAYNNNNRTG | 3 cut(s) | 259, 450, 915 |
| MspA1I | CMGCKG | 2 cut(s) | 845, 869 |
| MspI | CCGG | 2 cut(s) | 99, 964 |
| MspR9I | CCNGG | 1 cut(s) | 623 |
| Mva1269I | GAATGC | 1 cut(s) | 685 |
| MvaI | CCWGG | 1 cut(s) | 623 |
| MvnI | CGCG | 5 cut(s) | 209, 465, 638, 845, 1052 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 358, 635, 947 |
| NarI | GGCGCC | 1 cut(s) | 950 |
| NcoI | CCATGG | 1 cut(s) | 910 |
| NdeII | GATC | 3 cut(s) | 960, 1003, 1041 |
| NheI | GCTAGC | 1 cut(s) | 410 |
| NlaIII | CATG | 5 cut(s) | 38, 320, 455, 737, 914 |
| NlaIV | GGNNCC | 5 cut(s) | 6, 193, 302, 329, 951 |
| NmuCI | GTSAC | 3 cut(s) | 101, 252, 838 |
| NspI | RCATGY | 2 cut(s) | 320, 455 |
| PaeI | GCATGC | 2 cut(s) | 320, 455 |
| PagI | TCATGA | 1 cut(s) | 34 |
| PaqCI | CACCTGC | 1 cut(s) | 294 |
| PciSI | GCTCTTC | 1 cut(s) | 687 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 887 |
| PctI | GAATGC | 1 cut(s) | 685 |
| PfeI | GAWTC | 1 cut(s) | 122 |
| PinAI | ACCGGT | 1 cut(s) | 98 |
| PkrI | GCNGC | 6 cut(s) | 360, 583, 699, 847, 868, 1051 |
| PleI | GAGTC | 1 cut(s) | 604 |
| PluTI | GGCGCC | 1 cut(s) | 953 |
| PpsI | GAGTC | 1 cut(s) | 604 |
| PpuMI | RGGWCCY | 1 cut(s) | 328 |
| Psp5II | RGGWCCY | 1 cut(s) | 328 |
| Psp6I | CCWGG | 1 cut(s) | 621 |
| PspEI | GGTNACC | 2 cut(s) | 101, 838 |
| PspFI | CCCAGC | 1 cut(s) | 585 |
| PspGI | CCWGG | 1 cut(s) | 621 |
| PspN4I | GGNNCC | 5 cut(s) | 6, 193, 302, 329, 951 |
| PspPI | GGNCC | 4 cut(s) | 175, 328, 763, 1047 |
| PspPPI | RGGWCCY | 1 cut(s) | 328 |
| RsaI | GTAC | 3 cut(s) | 15, 205, 249 |
| RsaNI | GTAC | 3 cut(s) | 14, 204, 248 |
| RseI | CAYNNNNRTG | 3 cut(s) | 259, 450, 915 |
| SacII | CCGCGG | 1 cut(s) | 846 |
| SapI | GCTCTTC | 1 cut(s) | 687 |
| SaqAI | TTAA | 5 cut(s) | 18, 45, 404, 702, 933 |
| SatI | GCNGC | 6 cut(s) | 359, 582, 698, 846, 867, 1050 |
| Sau3AI | GATC | 3 cut(s) | 960, 1003, 1041 |
| Sau96I | GGNCC | 4 cut(s) | 175, 328, 763, 1047 |
| ScaI | AGTACT | 2 cut(s) | 15, 249 |
| SchI | GAGTC | 1 cut(s) | 605 |
| ScrFI | CCNGG | 1 cut(s) | 623 |
| SfaNI | GCATC | 2 cut(s) | 595, 624 |
| SfcI | CTRYAG | 1 cut(s) | 199 |
| SfoI | GGCGCC | 1 cut(s) | 951 |
| Sfr303I | CCGCGG | 1 cut(s) | 846 |
| SgrBI | CCGCGG | 1 cut(s) | 846 |
| SinI | GGWCC | 3 cut(s) | 175, 328, 763 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 259, 450, 915 |
| SphI | GCATGC | 2 cut(s) | 320, 455 |
| Sse9I | AATT | 1 cut(s) | 718 |
| SspDI | GGCGCC | 1 cut(s) | 949 |
| SspMI | CTAG | 6 cut(s) | 179, 230, 411, 612, 689, 1057 |
| StyD4I | CCNGG | 1 cut(s) | 621 |
| StyI | CCWWGG | 2 cut(s) | 186, 910 |
| TaaI | ACNGT | 6 cut(s) | 200, 220, 247, 252, 461, 982 |
| TaiI | ACGT | 2 cut(s) | 404, 881 |
| TaqI | TCGA | 4 cut(s) | 515, 547, 794, 890 |
| TaqII | GACCGA | 1 cut(s) | 163 |
| TasI | AATT | 1 cut(s) | 718 |
| TatI | WGTACW | 2 cut(s) | 13, 247 |
| TauI | GCSGC | 3 cut(s) | 584, 848, 1052 |
| TfiI | GAWTC | 1 cut(s) | 122 |
| Tru1I | TTAA | 5 cut(s) | 18, 45, 404, 702, 933 |
| Tru9I | TTAA | 5 cut(s) | 18, 45, 404, 702, 933 |
| TscAI | CASTG | 5 cut(s) | 149, 329, 813, 963, 987 |
| TseFI | GTSAC | 3 cut(s) | 101, 252, 838 |
| TseI | GCWGC | 3 cut(s) | 358, 697, 866 |
| Tsp45I | GTSAC | 3 cut(s) | 101, 252, 838 |
| TspRI | CASTG | 5 cut(s) | 149, 329, 813, 963, 987 |
| VpaK11BI | GGWCC | 3 cut(s) | 175, 328, 763 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 758 |
| XapI | RAATTY | 1 cut(s) | 718 |
| XceI | RCATGY | 2 cut(s) | 320, 455 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 220 |
| XspI | CTAG | 6 cut(s) | 179, 230, 411, 612, 689, 1057 |
| ZrmI | AGTACT | 2 cut(s) | 15, 249 |