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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 500 |
| AccII | CGCG | 2 cut(s) | 153, 378 |
| AclWI | GGATC | 1 cut(s) | 78 |
| AcoI | YGGCCR | 2 cut(s) | 110, 370 |
| AfaI | GTAC | 2 cut(s) | 134, 435 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 56, 246, 413, 414, 415, 651 |
| AgeI | ACCGGT | 1 cut(s) | 651 |
| AgsI | TTSAA | 2 cut(s) | 47, 211 |
| AjnI | CCWGG | 1 cut(s) | 549 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 160, 192 |
| AluBI | AGCT | 1 cut(s) | 366 |
| AluI | AGCT | 1 cut(s) | 366 |
| Alw21I | GWGCWC | 1 cut(s) | 574 |
| Alw26I | GTCTC | 1 cut(s) | 728 |
| AlwI | GGATC | 1 cut(s) | 78 |
| Ama87I | CYCGRG | 1 cut(s) | 731 |
| AoxI | GGCC | 6 cut(s) | 5, 73, 110, 159, 370, 552 |
| ApeKI | GCWGC | 1 cut(s) | 197 |
| AsiGI | ACCGGT | 1 cut(s) | 651 |
| AspLEI | GCGC | 4 cut(s) | 153, 245, 307, 316 |
| AspS9I | GGNCC | 2 cut(s) | 5, 398 |
| AsuC2I | CCSGG | 1 cut(s) | 240 |
| AsuHPI | GGTGA | 3 cut(s) | 549, 676, 712 |
| AvaI | CYCGRG | 1 cut(s) | 731 |
| AvaII | GGWCC | 1 cut(s) | 398 |
| BalI | TGGCCA | 1 cut(s) | 112 |
| BauI | CACGAG | 2 cut(s) | 481, 610 |
| Bbv12I | GWGCWC | 1 cut(s) | 574 |
| BbvI | GCAGC | 1 cut(s) | 184 |
| BccI | CCATC | 2 cut(s) | 125, 734 |
| BceAI | ACGGC | 1 cut(s) | 397 |
| BciT130I | CCWGG | 1 cut(s) | 551 |
| BciVI | GTATCC | 1 cut(s) | 607 |
| BcnI | CCSGG | 1 cut(s) | 240 |
| BcoDI | GTCTC | 1 cut(s) | 728 |
| BfuAI | ACCTGC | 1 cut(s) | 500 |
| BfuI | GTATCC | 1 cut(s) | 607 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 13, 148 |
| BisI | GCNGC | 5 cut(s) | 140, 195, 198, 278, 607 |
| BlsI | GCNGC | 5 cut(s) | 141, 196, 199, 279, 608 |
| Bme1390I | CCNGG | 2 cut(s) | 240, 551 |
| Bme18I | GGWCC | 1 cut(s) | 398 |
| BmeT110I | CYCGRG | 1 cut(s) | 731 |
| BmgT120I | GGNCC | 2 cut(s) | 5, 398 |
| BmiI | GGNNCC | 3 cut(s) | 6, 400, 642 |
| BmrFI | CCNGG | 2 cut(s) | 240, 551 |
| BmsI | GCATC | 3 cut(s) | 55, 325, 401 |
| BoxI | GACNNNNGTC | 1 cut(s) | 333 |
| BpuMI | CCSGG | 1 cut(s) | 240 |
| Bsa29I | ATCGAT | 1 cut(s) | 521 |
| BsaAI | YACGTR | 1 cut(s) | 136 |
| BsaJI | CCNNGG | 3 cut(s) | 76, 98, 561 |
| BsaWI | WCCGGW | 3 cut(s) | 120, 165, 651 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 56, 246, 413, 414, 415, 651 |
| Bse118I | RCCGGY | 1 cut(s) | 651 |
| BseBI | CCWGG | 1 cut(s) | 551 |
| BseCI | ATCGAT | 1 cut(s) | 521 |
| BseDI | CCNNGG | 3 cut(s) | 76, 98, 561 |
| BseGI | GGATG | 3 cut(s) | 117, 398, 726 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 56, 246, 413, 414, 415, 651 |
| BseXI | GCAGC | 1 cut(s) | 184 |
| Bsh1236I | CGCG | 2 cut(s) | 153, 378 |
| Bsh1285I | CGRYCG | 1 cut(s) | 656 |
| BshFI | GGCC | 6 cut(s) | 7, 75, 112, 161, 372, 554 |
| BshTI | ACCGGT | 1 cut(s) | 651 |
| BshVI | ATCGAT | 1 cut(s) | 521 |
| BsiEI | CGRYCG | 1 cut(s) | 656 |
| BsiHKAI | GWGCWC | 1 cut(s) | 574 |
| BsiHKCI | CYCGRG | 1 cut(s) | 731 |
| BsiSI | CCGG | 6 cut(s) | 121, 166, 240, 369, 470, 652 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 56, 246, 413, 414, 415, 651 |
| BsmAI | GTCTC | 1 cut(s) | 728 |
| BsnI | GGCC | 6 cut(s) | 7, 75, 112, 161, 372, 554 |
| BsoBI | CYCGRG | 1 cut(s) | 731 |
| Bsp1286I | GDGCHC | 1 cut(s) | 574 |
| Bsp1407I | TGTACA | 1 cut(s) | 433 |
| Bsp143I | GATC | 1 cut(s) | 83 |
| BspANI | GGCC | 6 cut(s) | 7, 75, 112, 161, 372, 554 |
| BspDI | ATCGAT | 1 cut(s) | 521 |
| BspFNI | CGCG | 2 cut(s) | 153, 378 |
| BspLI | GGNNCC | 3 cut(s) | 6, 400, 642 |
| BspMI | ACCTGC | 1 cut(s) | 500 |
| BspPI | GGATC | 1 cut(s) | 78 |
| BsrFI | RCCGGY | 1 cut(s) | 651 |
| BsrGI | TGTACA | 1 cut(s) | 433 |
| BssAI | RCCGGY | 1 cut(s) | 651 |
| BssECI | CCNNGG | 3 cut(s) | 76, 98, 561 |
| BssMI | GATC | 1 cut(s) | 83 |
| BssSI | CACGAG | 2 cut(s) | 481, 610 |
| BssT1I | CCWWGG | 2 cut(s) | 76, 98 |
| Bst2BI | CACGAG | 2 cut(s) | 481, 610 |
| Bst2UI | CCWGG | 1 cut(s) | 551 |
| BstAUI | TGTACA | 1 cut(s) | 433 |
| BstBAI | YACGTR | 1 cut(s) | 136 |
| BstC8I | GCNNGC | 1 cut(s) | 114 |
| BstDSI | CCRYGG | 1 cut(s) | 561 |
| BstF5I | GGATG | 3 cut(s) | 117, 398, 726 |
| BstFNI | CGCG | 2 cut(s) | 153, 378 |
| BstHHI | GCGC | 4 cut(s) | 153, 245, 307, 316 |
| BstKTI | GATC | 1 cut(s) | 86 |
| BstMAI | GTCTC | 1 cut(s) | 728 |
| BstMBI | GATC | 1 cut(s) | 83 |
| BstMCI | CGRYCG | 1 cut(s) | 656 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 13, 109, 148, 311, 313, 320, 363 |
| BstNI | CCWGG | 1 cut(s) | 551 |
| BstNSI | RCATGY | 1 cut(s) | 277 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 333 |
| BstSCI | CCNGG | 2 cut(s) | 238, 549 |
| BstUI | CGCG | 2 cut(s) | 153, 378 |
| BstV1I | GCAGC | 1 cut(s) | 184 |
| Bsu15I | ATCGAT | 1 cut(s) | 521 |
| BsuI | GTATCC | 1 cut(s) | 607 |
| BsuRI | GGCC | 6 cut(s) | 7, 75, 112, 161, 372, 554 |
| BsuTUI | ATCGAT | 1 cut(s) | 521 |
| BtgI | CCRYGG | 1 cut(s) | 561 |
| BtsCI | GGATG | 3 cut(s) | 117, 398, 726 |
| BveI | ACCTGC | 1 cut(s) | 500 |
| Cac8I | GCNNGC | 1 cut(s) | 114 |
| CfoI | GCGC | 4 cut(s) | 153, 245, 307, 316 |
| Cfr10I | RCCGGY | 1 cut(s) | 651 |
| Cfr13I | GGNCC | 2 cut(s) | 5, 398 |
| ClaI | ATCGAT | 1 cut(s) | 521 |
| Csp6I | GTAC | 2 cut(s) | 133, 434 |
| CspAI | ACCGGT | 1 cut(s) | 651 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 5, 40 |
| CviAII | CATG | 2 cut(s) | 51, 274 |
| CviQI | GTAC | 2 cut(s) | 133, 434 |
| DpnI | GATC | 1 cut(s) | 85 |
| DpnII | GATC | 1 cut(s) | 83 |
| EaeI | YGGCCR | 2 cut(s) | 110, 370 |
| Eco130I | CCWWGG | 2 cut(s) | 76, 98 |
| Eco147I | AGGCCT | 3 cut(s) | 75, 161, 554 |
| Eco47I | GGWCC | 1 cut(s) | 398 |
| Eco88I | CYCGRG | 1 cut(s) | 731 |
| EcoRII | CCWGG | 1 cut(s) | 549 |
| EcoT14I | CCWWGG | 2 cut(s) | 76, 98 |
| ErhI | CCWWGG | 2 cut(s) | 76, 98 |
| FaeI | CATG | 2 cut(s) | 54, 277 |
| FaiI | YATR | 6 cut(s) | 31, 52, 275, 288, 327, 425 |
| FatI | CATG | 2 cut(s) | 50, 273 |
| FauI | CCCGC | 2 cut(s) | 407, 653 |
| Fnu4HI | GCNGC | 5 cut(s) | 140, 195, 198, 278, 607 |
| FokI | GGATG | 3 cut(s) | 104, 405, 713 |
| Fsp4HI | GCNGC | 5 cut(s) | 140, 195, 198, 278, 607 |
| GlaI | GCGC | 4 cut(s) | 152, 244, 306, 315 |
| GluI | GCNGC | 5 cut(s) | 140, 195, 198, 278, 607 |
| HaeIII | GGCC | 6 cut(s) | 7, 75, 112, 161, 372, 554 |
| HapII | CCGG | 6 cut(s) | 121, 166, 240, 369, 470, 652 |
| HhaI | GCGC | 4 cut(s) | 153, 245, 307, 316 |
| Hin1II | CATG | 2 cut(s) | 54, 277 |
| Hin6I | GCGC | 4 cut(s) | 151, 243, 305, 314 |
| HinP1I | GCGC | 4 cut(s) | 151, 243, 305, 314 |
| HinfI | GANTC | 3 cut(s) | 145, 500, 736 |
| HpaII | CCGG | 6 cut(s) | 121, 166, 240, 369, 470, 652 |
| HphI | GGTGA | 3 cut(s) | 549, 676, 712 |
| Hpy166II | GTNNAC | 2 cut(s) | 271, 398 |
| Hpy188III | TCNNGA | 3 cut(s) | 497, 575, 750 |
| Hpy8I | GTNNAC | 2 cut(s) | 271, 398 |
| Hpy99I | CGWCG | 4 cut(s) | 187, 383, 544, 675 |
| HpyAV | CCTTC | 2 cut(s) | 50, 413 |
| HpyCH4IV | ACGT | 2 cut(s) | 135, 348 |
| HpyCH4V | TGCA | 1 cut(s) | 68 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 13, 109, 148, 311, 313, 320, 363 |
| HpySE526I | ACGT | 2 cut(s) | 135, 348 |
| Hsp92II | CATG | 2 cut(s) | 54, 277 |
| HspAI | GCGC | 4 cut(s) | 151, 243, 305, 314 |
| Kzo9I | GATC | 1 cut(s) | 83 |
| LmnI | GCTCC | 1 cut(s) | 371 |
| Lsp1109I | GCAGC | 1 cut(s) | 184 |
| LweI | GCATC | 3 cut(s) | 55, 325, 401 |
| MaeII | ACGT | 2 cut(s) | 135, 348 |
| MaeIII | GTNAC | 2 cut(s) | 494, 713 |
| MalI | GATC | 1 cut(s) | 85 |
| MboI | GATC | 1 cut(s) | 83 |
| MboII | GAAGA | 2 cut(s) | 171, 223 |
| MhlI | GDGCHC | 1 cut(s) | 574 |
| MlsI | TGGCCA | 1 cut(s) | 112 |
| MluCI | AATT | 4 cut(s) | 42, 351, 534, 754 |
| MluNI | TGGCCA | 1 cut(s) | 112 |
| MlyI | GAGTC | 1 cut(s) | 730 |
| MnlI | CCTC | 5 cut(s) | 304, 306, 565, 579, 606 |
| Mox20I | TGGCCA | 1 cut(s) | 112 |
| MscI | TGGCCA | 1 cut(s) | 112 |
| MseI | TTAA | 2 cut(s) | 342, 525 |
| Msp20I | TGGCCA | 1 cut(s) | 112 |
| MspA1I | CMGCKG | 1 cut(s) | 197 |
| MspI | CCGG | 6 cut(s) | 121, 166, 240, 369, 470, 652 |
| MspR9I | CCNGG | 2 cut(s) | 240, 551 |
| MvaI | CCWGG | 1 cut(s) | 551 |
| MvnI | CGCG | 2 cut(s) | 153, 378 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 13, 109, 148, 311, 313, 320, 363 |
| NciI | CCSGG | 1 cut(s) | 240 |
| NdeII | GATC | 1 cut(s) | 83 |
| NlaIII | CATG | 2 cut(s) | 54, 277 |
| NlaIV | GGNNCC | 3 cut(s) | 6, 400, 642 |
| NmuCI | GTSAC | 2 cut(s) | 494, 713 |
| NspI | RCATGY | 1 cut(s) | 277 |
| PceI | AGGCCT | 3 cut(s) | 75, 161, 554 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 132 |
| PfeI | GAWTC | 2 cut(s) | 145, 500 |
| PinAI | ACCGGT | 1 cut(s) | 651 |
| PkrI | GCNGC | 5 cut(s) | 141, 196, 199, 279, 608 |
| PleI | GAGTC | 1 cut(s) | 730 |
| PpsI | GAGTC | 1 cut(s) | 730 |
| Ppu21I | YACGTR | 1 cut(s) | 136 |
| PshAI | GACNNNNGTC | 1 cut(s) | 333 |
| Psp6I | CCWGG | 1 cut(s) | 549 |
| PspGI | CCWGG | 1 cut(s) | 549 |
| PspN4I | GGNNCC | 3 cut(s) | 6, 400, 642 |
| PspPI | GGNCC | 2 cut(s) | 5, 398 |
| RsaI | GTAC | 2 cut(s) | 134, 435 |
| RsaNI | GTAC | 2 cut(s) | 133, 434 |
| SaqAI | TTAA | 2 cut(s) | 342, 525 |
| SatI | GCNGC | 5 cut(s) | 140, 195, 198, 278, 607 |
| Sau3AI | GATC | 1 cut(s) | 83 |
| Sau96I | GGNCC | 2 cut(s) | 5, 398 |
| SchI | GAGTC | 1 cut(s) | 730 |
| ScrFI | CCNGG | 2 cut(s) | 240, 551 |
| SduI | GDGCHC | 1 cut(s) | 574 |
| SetI | ASST | 5 cut(s) | 138, 230, 351, 368, 514 |
| SfaNI | GCATC | 3 cut(s) | 55, 325, 401 |
| SinI | GGWCC | 1 cut(s) | 398 |
| Sse9I | AATT | 4 cut(s) | 42, 351, 534, 754 |
| SseBI | AGGCCT | 3 cut(s) | 75, 161, 554 |
| StuI | AGGCCT | 3 cut(s) | 75, 161, 554 |
| StyD4I | CCNGG | 2 cut(s) | 238, 549 |
| StyI | CCWWGG | 2 cut(s) | 76, 98 |
| TaiI | ACGT | 2 cut(s) | 138, 351 |
| TaqI | TCGA | 5 cut(s) | 185, 319, 521, 576, 656 |
| TasI | AATT | 4 cut(s) | 42, 351, 534, 754 |
| TatI | WGTACW | 1 cut(s) | 433 |
| TauI | GCSGC | 4 cut(s) | 142, 197, 280, 609 |
| TfiI | GAWTC | 2 cut(s) | 145, 500 |
| Tru1I | TTAA | 2 cut(s) | 342, 525 |
| Tru9I | TTAA | 2 cut(s) | 342, 525 |
| TseFI | GTSAC | 2 cut(s) | 494, 713 |
| TseI | GCWGC | 1 cut(s) | 197 |
| Tsp45I | GTSAC | 2 cut(s) | 494, 713 |
| TspDTI | ATGAA | 1 cut(s) | 251 |
| TspGWI | ACGGA | 1 cut(s) | 472 |
| VpaK11BI | GGWCC | 1 cut(s) | 398 |
| XceI | RCATGY | 1 cut(s) | 277 |