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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 461 |
| AclWI | GGATC | 2 cut(s) | 359, 484 |
| AcsI | RAATTY | 1 cut(s) | 449 |
| AcuI | CTGAAG | 2 cut(s) | 408, 596 |
| AfaI | GTAC | 2 cut(s) | 173, 316 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 535 |
| AgsI | TTSAA | 1 cut(s) | 22 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 239 |
| AjnI | CCWGG | 1 cut(s) | 91 |
| AluBI | AGCT | 4 cut(s) | 74, 422, 428, 616 |
| AluI | AGCT | 4 cut(s) | 74, 422, 428, 616 |
| Alw26I | GTCTC | 2 cut(s) | 226, 252 |
| AlwI | GGATC | 2 cut(s) | 359, 484 |
| AoxI | GGCC | 1 cut(s) | 662 |
| ApeKI | GCWGC | 2 cut(s) | 163, 212 |
| ApoI | RAATTY | 1 cut(s) | 449 |
| AspS9I | GGNCC | 2 cut(s) | 635, 662 |
| AsuC2I | CCSGG | 1 cut(s) | 494 |
| AvaII | GGWCC | 1 cut(s) | 635 |
| BanII | GRGCYC | 1 cut(s) | 484 |
| BbvI | GCAGC | 2 cut(s) | 175, 224 |
| BccI | CCATC | 1 cut(s) | 471 |
| BciT130I | CCWGG | 1 cut(s) | 93 |
| BciVI | GTATCC | 1 cut(s) | 490 |
| BcnI | CCSGG | 1 cut(s) | 494 |
| BcoDI | GTCTC | 2 cut(s) | 226, 252 |
| BfmI | CTRYAG | 1 cut(s) | 120 |
| BfuI | GTATCC | 1 cut(s) | 490 |
| BisI | GCNGC | 2 cut(s) | 164, 213 |
| BlsI | GCNGC | 2 cut(s) | 165, 214 |
| Bme1390I | CCNGG | 2 cut(s) | 93, 494 |
| Bme18I | GGWCC | 1 cut(s) | 635 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 239 |
| BmgT120I | GGNCC | 2 cut(s) | 635, 662 |
| BmrFI | CCNGG | 2 cut(s) | 93, 494 |
| BpmI | CTGGAG | 1 cut(s) | 114 |
| BpuMI | CCSGG | 1 cut(s) | 494 |
| BsaBI | GATNNNNATC | 1 cut(s) | 363 |
| BsaJI | CCNNGG | 2 cut(s) | 35, 530 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 535 |
| Bse1I | ACTGG | 1 cut(s) | 275 |
| Bse3DI | GCAATG | 2 cut(s) | 504, 654 |
| Bse8I | GATNNNNATC | 1 cut(s) | 363 |
| BseBI | CCWGG | 1 cut(s) | 93 |
| BseDI | CCNNGG | 2 cut(s) | 35, 530 |
| BseJI | GATNNNNATC | 1 cut(s) | 363 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 535 |
| BseMI | GCAATG | 2 cut(s) | 504, 654 |
| BseMII | CTCAG | 2 cut(s) | 66, 271 |
| BseNI | ACTGG | 1 cut(s) | 275 |
| BseRI | GAGGAG | 2 cut(s) | 215, 218 |
| BseXI | GCAGC | 2 cut(s) | 175, 224 |
| BsgI | GTGCAG | 1 cut(s) | 643 |
| BshFI | GGCC | 1 cut(s) | 664 |
| BsiSI | CCGG | 1 cut(s) | 494 |
| BslFI | GGGAC | 1 cut(s) | 527 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 535 |
| BsmAI | GTCTC | 2 cut(s) | 226, 252 |
| BsmFI | GGGAC | 1 cut(s) | 527 |
| BsnI | GGCC | 1 cut(s) | 664 |
| Bsp1286I | GDGCHC | 1 cut(s) | 484 |
| Bsp143I | GATC | 3 cut(s) | 51, 364, 489 |
| BspANI | GGCC | 1 cut(s) | 664 |
| BspCNI | CTCAG | 2 cut(s) | 67, 270 |
| BspPI | GGATC | 2 cut(s) | 359, 484 |
| BspQI | GCTCTTC | 1 cut(s) | 64 |
| BsrDI | GCAATG | 2 cut(s) | 504, 654 |
| BsrI | ACTGG | 1 cut(s) | 275 |
| BssECI | CCNNGG | 2 cut(s) | 35, 530 |
| BssMI | GATC | 3 cut(s) | 51, 364, 489 |
| BssNAI | GTATAC | 1 cut(s) | 462 |
| BssT1I | CCWWGG | 1 cut(s) | 530 |
| Bst1107I | GTATAC | 1 cut(s) | 462 |
| Bst2UI | CCWGG | 1 cut(s) | 93 |
| Bst4CI | ACNGT | 2 cut(s) | 39, 241 |
| Bst6I | CTCTTC | 1 cut(s) | 64 |
| BstDEI | CTNAG | 3 cut(s) | 75, 257, 305 |
| BstDSI | CCRYGG | 1 cut(s) | 35 |
| BstKTI | GATC | 3 cut(s) | 54, 367, 492 |
| BstMAI | GTCTC | 2 cut(s) | 226, 252 |
| BstMBI | GATC | 3 cut(s) | 51, 364, 489 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 479 |
| BstNI | CCWGG | 1 cut(s) | 93 |
| BstSCI | CCNGG | 2 cut(s) | 91, 492 |
| BstSFI | CTRYAG | 1 cut(s) | 120 |
| BstV1I | GCAGC | 2 cut(s) | 175, 224 |
| BstX2I | RGATCY | 1 cut(s) | 364 |
| BstYI | RGATCY | 1 cut(s) | 364 |
| BstZ17I | GTATAC | 1 cut(s) | 462 |
| BsuI | GTATCC | 1 cut(s) | 490 |
| BsuRI | GGCC | 1 cut(s) | 664 |
| BtgI | CCRYGG | 1 cut(s) | 35 |
| BtsI | GCAGTG | 3 cut(s) | 136, 390, 631 |
| BtsIMutI | CAGTG | 3 cut(s) | 136, 390, 631 |
| Cfr13I | GGNCC | 2 cut(s) | 635, 662 |
| Csp6I | GTAC | 2 cut(s) | 172, 315 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 366, 401 |
| CviAII | CATG | 3 cut(s) | 176, 351, 485 |
| CviQI | GTAC | 2 cut(s) | 172, 315 |
| DdeI | CTNAG | 3 cut(s) | 75, 257, 305 |
| DpnI | GATC | 3 cut(s) | 53, 366, 491 |
| DpnII | GATC | 3 cut(s) | 51, 364, 489 |
| DriI | GACNNNNNGTC | 1 cut(s) | 239 |
| Eam1104I | CTCTTC | 1 cut(s) | 64 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 239 |
| EarI | CTCTTC | 1 cut(s) | 64 |
| Eco130I | CCWWGG | 1 cut(s) | 530 |
| Eco24I | GRGCYC | 1 cut(s) | 484 |
| Eco47I | GGWCC | 1 cut(s) | 635 |
| Eco57I | CTGAAG | 2 cut(s) | 408, 596 |
| EcoO109I | RGGNCCY | 1 cut(s) | 662 |
| EcoRII | CCWGG | 1 cut(s) | 91 |
| EcoT14I | CCWWGG | 1 cut(s) | 530 |
| EcoT38I | GRGCYC | 1 cut(s) | 484 |
| ErhI | CCWWGG | 1 cut(s) | 530 |
| FaeI | CATG | 3 cut(s) | 179, 354, 488 |
| FaqI | GGGAC | 1 cut(s) | 527 |
| FatI | CATG | 3 cut(s) | 175, 350, 484 |
| FblI | GTMKAC | 1 cut(s) | 461 |
| Fnu4HI | GCNGC | 2 cut(s) | 164, 213 |
| FriOI | GRGCYC | 1 cut(s) | 484 |
| Fsp4HI | GCNGC | 2 cut(s) | 164, 213 |
| GluI | GCNGC | 2 cut(s) | 164, 213 |
| GsuI | CTGGAG | 1 cut(s) | 114 |
| HaeIII | GGCC | 1 cut(s) | 664 |
| HapII | CCGG | 1 cut(s) | 494 |
| Hin1II | CATG | 3 cut(s) | 179, 354, 488 |
| HincII | GTYRAC | 1 cut(s) | 656 |
| HindII | GTYRAC | 1 cut(s) | 656 |
| HindIII | AAGCTT | 1 cut(s) | 420 |
| HinfI | GANTC | 3 cut(s) | 261, 600, 641 |
| HpaII | CCGG | 1 cut(s) | 494 |
| Hpy166II | GTNNAC | 3 cut(s) | 462, 599, 656 |
| Hpy188I | TCNGA | 1 cut(s) | 260 |
| Hpy188III | TCNNGA | 1 cut(s) | 62 |
| Hpy8I | GTNNAC | 3 cut(s) | 462, 599, 656 |
| Hpy99I | CGWCG | 2 cut(s) | 68, 103 |
| HpyAV | CCTTC | 2 cut(s) | 73, 379 |
| HpyCH4III | ACNGT | 2 cut(s) | 39, 241 |
| HpyCH4V | TGCA | 4 cut(s) | 212, 473, 509, 624 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 479 |
| HpyF3I | CTNAG | 3 cut(s) | 75, 257, 305 |
| Hsp92II | CATG | 3 cut(s) | 179, 354, 488 |
| Kzo9I | GATC | 3 cut(s) | 51, 364, 489 |
| LguI | GCTCTTC | 1 cut(s) | 64 |
| LmnI | GCTCC | 1 cut(s) | 95 |
| LpnPI | CCDG | 9 cut(s) | 78, 105, 213, 288, 303, 323, 450, 507, 651 |
| Lsp1109I | GCAGC | 2 cut(s) | 175, 224 |
| MaeIII | GTNAC | 1 cut(s) | 188 |
| MalI | GATC | 3 cut(s) | 53, 366, 491 |
| MboI | GATC | 3 cut(s) | 51, 364, 489 |
| MboII | GAAGA | 5 cut(s) | 81, 281, 402, 524, 566 |
| MflI | RGATCY | 1 cut(s) | 364 |
| MhlI | GDGCHC | 1 cut(s) | 484 |
| MluCI | AATT | 1 cut(s) | 449 |
| MlyI | GAGTC | 1 cut(s) | 594 |
| MnlI | CCTC | 9 cut(s) | 54, 99, 109, 193, 196, 199, 266, 315, 653 |
| MseI | TTAA | 2 cut(s) | 279, 630 |
| MspI | CCGG | 1 cut(s) | 494 |
| MspR9I | CCNGG | 2 cut(s) | 93, 494 |
| MvaI | CCWGG | 1 cut(s) | 93 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 479 |
| NciI | CCSGG | 1 cut(s) | 494 |
| NdeII | GATC | 3 cut(s) | 51, 364, 489 |
| NlaIII | CATG | 3 cut(s) | 179, 354, 488 |
| PciSI | GCTCTTC | 1 cut(s) | 64 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 60 |
| PfeI | GAWTC | 2 cut(s) | 261, 641 |
| PfoI | TCCNGGA | 2 cut(s) | 91, 492 |
| PkrI | GCNGC | 2 cut(s) | 165, 214 |
| PleI | GAGTC | 1 cut(s) | 594 |
| PpsI | GAGTC | 1 cut(s) | 594 |
| Psp6I | CCWGG | 1 cut(s) | 91 |
| PspGI | CCWGG | 1 cut(s) | 91 |
| PspPI | GGNCC | 2 cut(s) | 635, 662 |
| PsuI | RGATCY | 1 cut(s) | 364 |
| RsaI | GTAC | 2 cut(s) | 173, 316 |
| RsaNI | GTAC | 2 cut(s) | 172, 315 |
| SapI | GCTCTTC | 1 cut(s) | 64 |
| SaqAI | TTAA | 2 cut(s) | 279, 630 |
| SatI | GCNGC | 2 cut(s) | 164, 213 |
| Sau3AI | GATC | 3 cut(s) | 51, 364, 489 |
| Sau96I | GGNCC | 2 cut(s) | 635, 662 |
| SchI | GAGTC | 1 cut(s) | 594 |
| ScrFI | CCNGG | 2 cut(s) | 93, 494 |
| SduI | GDGCHC | 1 cut(s) | 484 |
| SetI | ASST | 8 cut(s) | 76, 210, 326, 424, 430, 550, 577, 618 |
| SfcI | CTRYAG | 1 cut(s) | 120 |
| SinI | GGWCC | 1 cut(s) | 635 |
| Sse9I | AATT | 1 cut(s) | 449 |
| StyD4I | CCNGG | 2 cut(s) | 91, 492 |
| StyI | CCWWGG | 1 cut(s) | 530 |
| TaaI | ACNGT | 2 cut(s) | 39, 241 |
| TaqI | TCGA | 1 cut(s) | 54 |
| TasI | AATT | 1 cut(s) | 449 |
| TatI | WGTACW | 1 cut(s) | 314 |
| TfiI | GAWTC | 2 cut(s) | 261, 641 |
| Tru1I | TTAA | 2 cut(s) | 279, 630 |
| Tru9I | TTAA | 2 cut(s) | 279, 630 |
| TscAI | CASTG | 3 cut(s) | 136, 390, 631 |
| TseI | GCWGC | 2 cut(s) | 163, 212 |
| TspDTI | ATGAA | 5 cut(s) | 347, 354, 402, 567, 577 |
| TspGWI | ACGGA | 1 cut(s) | 81 |
| TspRI | CASTG | 3 cut(s) | 136, 390, 631 |
| VpaK11BI | GGWCC | 1 cut(s) | 635 |
| XapI | RAATTY | 1 cut(s) | 449 |
| XmiI | GTMKAC | 1 cut(s) | 461 |