No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 11 |
| AccB1I | GGYRCC | 1 cut(s) | 44 |
| AccIII | TCCGGA | 1 cut(s) | 283 |
| AciI | CCGC | 6 cut(s) | 48, 51, 86, 107, 194, 272 |
| AclWI | GGATC | 1 cut(s) | 143 |
| AcoI | YGGCCR | 1 cut(s) | 92 |
| AcyI | GRCGYC | 2 cut(s) | 8, 45 |
| AfaI | GTAC | 1 cut(s) | 412 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 54, 181 |
| AgsI | TTSAA | 3 cut(s) | 218, 499, 509 |
| AjnI | CCWGG | 1 cut(s) | 418 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 96, 128 |
| AluBI | AGCT | 1 cut(s) | 505 |
| AluI | AGCT | 1 cut(s) | 505 |
| Alw26I | GTCTC | 3 cut(s) | 113, 202, 371 |
| AlwI | GGATC | 1 cut(s) | 143 |
| Ama87I | CYCGRG | 1 cut(s) | 248 |
| Aor13HI | TCCGGA | 1 cut(s) | 283 |
| AoxI | GGCC | 5 cut(s) | 71, 92, 153, 171, 227 |
| ApeKI | GCWGC | 1 cut(s) | 461 |
| AspLEI | GCGC | 4 cut(s) | 47, 213, 309, 316 |
| AspS9I | GGNCC | 2 cut(s) | 172, 281 |
| AsuC2I | CCSGG | 2 cut(s) | 75, 478 |
| AsuHPI | GGTGA | 1 cut(s) | 434 |
| AvaI | CYCGRG | 1 cut(s) | 248 |
| AvaII | GGWCC | 1 cut(s) | 281 |
| BaeGI | GKGCMC | 1 cut(s) | 419 |
| BanI | GGYRCC | 1 cut(s) | 44 |
| BanII | GRGCYC | 3 cut(s) | 141, 291, 526 |
| BbvI | GCAGC | 1 cut(s) | 473 |
| BccI | CCATC | 2 cut(s) | 94, 323 |
| BciT130I | CCWGG | 1 cut(s) | 420 |
| BcnI | CCSGG | 2 cut(s) | 75, 478 |
| BcoDI | GTCTC | 3 cut(s) | 113, 202, 371 |
| BfoI | RGCGCY | 2 cut(s) | 48, 214 |
| BisI | GCNGC | 3 cut(s) | 48, 51, 462 |
| BlsI | GCNGC | 3 cut(s) | 49, 52, 463 |
| Bme1390I | CCNGG | 3 cut(s) | 75, 420, 478 |
| Bme18I | GGWCC | 1 cut(s) | 281 |
| BmeT110I | CYCGRG | 1 cut(s) | 248 |
| BmgT120I | GGNCC | 2 cut(s) | 172, 281 |
| BmiI | GGNNCC | 4 cut(s) | 46, 105, 288, 523 |
| BmrFI | CCNGG | 3 cut(s) | 75, 420, 478 |
| BmsI | GCATC | 1 cut(s) | 373 |
| BpuMI | CCSGG | 2 cut(s) | 75, 478 |
| BsaAI | YACGTR | 1 cut(s) | 414 |
| BsaHI | GRCGYC | 2 cut(s) | 8, 45 |
| BsaI | GGTCTC | 1 cut(s) | 202 |
| BsaJI | CCNNGG | 3 cut(s) | 74, 418, 517 |
| BsaWI | WCCGGW | 1 cut(s) | 283 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 54, 181 |
| Bse118I | RCCGGY | 2 cut(s) | 94, 181 |
| BseAI | TCCGGA | 1 cut(s) | 283 |
| BseBI | CCWGG | 1 cut(s) | 420 |
| BseDI | CCNNGG | 3 cut(s) | 74, 418, 517 |
| BseGI | GGATG | 1 cut(s) | 448 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 54, 181 |
| BseSI | GKGCMC | 1 cut(s) | 419 |
| BseXI | GCAGC | 1 cut(s) | 473 |
| Bsh1285I | CGRYCG | 2 cut(s) | 327, 477 |
| BshFI | GGCC | 5 cut(s) | 73, 94, 155, 173, 229 |
| BshNI | GGYRCC | 1 cut(s) | 44 |
| BsiEI | CGRYCG | 2 cut(s) | 327, 477 |
| BsiHKCI | CYCGRG | 1 cut(s) | 248 |
| BsiSI | CCGG | 7 cut(s) | 74, 95, 152, 182, 284, 428, 477 |
| BsiWI | CGTACG | 1 cut(s) | 410 |
| BslFI | GGGAC | 1 cut(s) | 91 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 54, 181 |
| BsmAI | GTCTC | 3 cut(s) | 113, 202, 371 |
| BsmBI | CGTCTC | 1 cut(s) | 371 |
| BsmFI | GGGAC | 1 cut(s) | 91 |
| BsmI | GAATGC | 1 cut(s) | 399 |
| BsnI | GGCC | 5 cut(s) | 73, 94, 155, 173, 229 |
| Bso31I | GGTCTC | 1 cut(s) | 202 |
| BsoBI | CYCGRG | 1 cut(s) | 248 |
| Bsp1286I | GDGCHC | 4 cut(s) | 141, 291, 419, 526 |
| Bsp13I | TCCGGA | 1 cut(s) | 283 |
| Bsp143I | GATC | 1 cut(s) | 148 |
| BspACI | CCGC | 6 cut(s) | 48, 51, 86, 107, 194, 272 |
| BspANI | GGCC | 5 cut(s) | 73, 94, 155, 173, 229 |
| BspEI | TCCGGA | 1 cut(s) | 283 |
| BspLI | GGNNCC | 4 cut(s) | 46, 105, 288, 523 |
| BspPI | GGATC | 1 cut(s) | 143 |
| BspT107I | GGYRCC | 1 cut(s) | 44 |
| BspTNI | GGTCTC | 1 cut(s) | 202 |
| BsrFI | RCCGGY | 2 cut(s) | 94, 181 |
| BssAI | RCCGGY | 2 cut(s) | 94, 181 |
| BssECI | CCNNGG | 3 cut(s) | 74, 418, 517 |
| BssMI | GATC | 1 cut(s) | 148 |
| BssNI | GRCGYC | 2 cut(s) | 8, 45 |
| BssT1I | CCWWGG | 1 cut(s) | 517 |
| Bst2UI | CCWGG | 1 cut(s) | 420 |
| Bst4CI | ACNGT | 2 cut(s) | 82, 325 |
| Bst6I | CTCTTC | 1 cut(s) | 432 |
| BstACI | GRCGYC | 2 cut(s) | 8, 45 |
| BstBAI | YACGTR | 1 cut(s) | 414 |
| BstC8I | GCNNGC | 2 cut(s) | 96, 183 |
| BstDEI | CTNAG | 2 cut(s) | 68, 371 |
| BstF5I | GGATG | 1 cut(s) | 448 |
| BstH2I | RGCGCY | 2 cut(s) | 48, 214 |
| BstHHI | GCGC | 4 cut(s) | 47, 213, 309, 316 |
| BstKTI | GATC | 1 cut(s) | 151 |
| BstMAI | GTCTC | 3 cut(s) | 113, 202, 371 |
| BstMBI | GATC | 1 cut(s) | 148 |
| BstMCI | CGRYCG | 2 cut(s) | 327, 477 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 191, 313, 361 |
| BstNI | CCWGG | 1 cut(s) | 420 |
| BstSCI | CCNGG | 3 cut(s) | 73, 418, 476 |
| BstSLI | GKGCMC | 1 cut(s) | 419 |
| BstV1I | GCAGC | 1 cut(s) | 473 |
| BstX2I | RGATCY | 1 cut(s) | 148 |
| BstYI | RGATCY | 1 cut(s) | 148 |
| BsuRI | GGCC | 5 cut(s) | 73, 94, 155, 173, 229 |
| BtgZI | GCGATG | 1 cut(s) | 113 |
| BtsCI | GGATG | 1 cut(s) | 448 |
| Cac8I | GCNNGC | 2 cut(s) | 96, 183 |
| CfoI | GCGC | 4 cut(s) | 47, 213, 309, 316 |
| Cfr10I | RCCGGY | 2 cut(s) | 94, 181 |
| Cfr13I | GGNCC | 2 cut(s) | 172, 281 |
| CseI | GACGC | 1 cut(s) | 391 |
| Csp6I | GTAC | 1 cut(s) | 411 |
| CviAII | CATG | 4 cut(s) | 54, 169, 310, 317 |
| CviQI | GTAC | 1 cut(s) | 411 |
| DdeI | CTNAG | 2 cut(s) | 68, 371 |
| DinI | GGCGCC | 1 cut(s) | 46 |
| DpnI | GATC | 1 cut(s) | 150 |
| DpnII | GATC | 1 cut(s) | 148 |
| EaeI | YGGCCR | 1 cut(s) | 92 |
| Eam1104I | CTCTTC | 1 cut(s) | 432 |
| EarI | CTCTTC | 1 cut(s) | 432 |
| EciI | GGCGGA | 1 cut(s) | 101 |
| Eco130I | CCWWGG | 1 cut(s) | 517 |
| Eco24I | GRGCYC | 3 cut(s) | 141, 291, 526 |
| Eco31I | GGTCTC | 1 cut(s) | 202 |
| Eco47I | GGWCC | 1 cut(s) | 281 |
| Eco88I | CYCGRG | 1 cut(s) | 248 |
| EcoRII | CCWGG | 1 cut(s) | 418 |
| EcoT14I | CCWWGG | 1 cut(s) | 517 |
| EcoT38I | GRGCYC | 3 cut(s) | 141, 291, 526 |
| EgeI | GGCGCC | 1 cut(s) | 46 |
| EheI | GGCGCC | 1 cut(s) | 46 |
| ErhI | CCWWGG | 1 cut(s) | 517 |
| Esp3I | CGTCTC | 1 cut(s) | 371 |
| FaeI | CATG | 4 cut(s) | 57, 172, 313, 320 |
| FaiI | YATR | 6 cut(s) | 55, 170, 311, 318, 458, 468 |
| FaqI | GGGAC | 1 cut(s) | 91 |
| FatI | CATG | 4 cut(s) | 53, 168, 309, 316 |
| Fnu4HI | GCNGC | 3 cut(s) | 48, 51, 462 |
| FokI | GGATG | 1 cut(s) | 455 |
| FriOI | GRGCYC | 3 cut(s) | 141, 291, 526 |
| Fsp4HI | GCNGC | 3 cut(s) | 48, 51, 462 |
| GlaI | GCGC | 4 cut(s) | 46, 212, 308, 315 |
| GluI | GCNGC | 3 cut(s) | 48, 51, 462 |
| HaeII | RGCGCY | 2 cut(s) | 48, 214 |
| HaeIII | GGCC | 5 cut(s) | 73, 94, 155, 173, 229 |
| HapII | CCGG | 7 cut(s) | 74, 95, 152, 182, 284, 428, 477 |
| HgaI | GACGC | 1 cut(s) | 391 |
| HhaI | GCGC | 4 cut(s) | 47, 213, 309, 316 |
| Hin1I | GRCGYC | 2 cut(s) | 8, 45 |
| Hin1II | CATG | 4 cut(s) | 57, 172, 313, 320 |
| Hin6I | GCGC | 4 cut(s) | 45, 211, 307, 314 |
| HinP1I | GCGC | 4 cut(s) | 45, 211, 307, 314 |
| HinfI | GANTC | 1 cut(s) | 22 |
| HpaII | CCGG | 7 cut(s) | 74, 95, 152, 182, 284, 428, 477 |
| HphI | GGTGA | 1 cut(s) | 434 |
| Hpy188I | TCNGA | 1 cut(s) | 224 |
| Hpy188III | TCNNGA | 1 cut(s) | 284 |
| Hpy99I | CGWCG | 1 cut(s) | 385 |
| HpyAV | CCTTC | 2 cut(s) | 219, 247 |
| HpyCH4III | ACNGT | 2 cut(s) | 82, 325 |
| HpyCH4IV | ACGT | 3 cut(s) | 8, 409, 413 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 191, 313, 361 |
| HpyF3I | CTNAG | 2 cut(s) | 68, 371 |
| HpySE526I | ACGT | 3 cut(s) | 8, 409, 413 |
| Hsp92I | GRCGYC | 2 cut(s) | 8, 45 |
| Hsp92II | CATG | 4 cut(s) | 57, 172, 313, 320 |
| HspAI | GCGC | 4 cut(s) | 45, 211, 307, 314 |
| KasI | GGCGCC | 1 cut(s) | 44 |
| Kpn2I | TCCGGA | 1 cut(s) | 283 |
| KroI | GCCGGC | 2 cut(s) | 94, 181 |
| KroNI | GCCGGC | 2 cut(s) | 96, 183 |
| Kzo9I | GATC | 1 cut(s) | 148 |
| LmnI | GCTCC | 2 cut(s) | 286, 521 |
| LpnPI | CCDG | 9 cut(s) | 87, 108, 165, 195, 297, 405, 432, 441, 490 |
| Lsp1109I | GCAGC | 1 cut(s) | 473 |
| LweI | GCATC | 1 cut(s) | 373 |
| MaeII | ACGT | 3 cut(s) | 8, 409, 413 |
| MalI | GATC | 1 cut(s) | 150 |
| MboI | GATC | 1 cut(s) | 148 |
| MboII | GAAGA | 5 cut(s) | 197, 402, 449, 493, 521 |
| MflI | RGATCY | 1 cut(s) | 148 |
| MhlI | GDGCHC | 4 cut(s) | 141, 291, 419, 526 |
| MluCI | AATT | 1 cut(s) | 188 |
| Mly113I | GGCGCC | 1 cut(s) | 45 |
| MnlI | CCTC | 5 cut(s) | 22, 166, 341, 433, 439 |
| MroI | TCCGGA | 1 cut(s) | 283 |
| MroNI | GCCGGC | 2 cut(s) | 94, 181 |
| MseI | TTAA | 3 cut(s) | 264, 276, 529 |
| MspI | CCGG | 7 cut(s) | 74, 95, 152, 182, 284, 428, 477 |
| MspR9I | CCNGG | 3 cut(s) | 75, 420, 478 |
| Mva1269I | GAATGC | 1 cut(s) | 399 |
| MvaI | CCWGG | 1 cut(s) | 420 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 191, 313, 361 |
| NaeI | GCCGGC | 2 cut(s) | 96, 183 |
| NarI | GGCGCC | 1 cut(s) | 45 |
| NciI | CCSGG | 2 cut(s) | 75, 478 |
| NdeII | GATC | 1 cut(s) | 148 |
| NgoMIV | GCCGGC | 2 cut(s) | 94, 181 |
| NlaIII | CATG | 4 cut(s) | 57, 172, 313, 320 |
| NlaIV | GGNNCC | 4 cut(s) | 46, 105, 288, 523 |
| PctI | GAATGC | 1 cut(s) | 399 |
| PdiI | GCCGGC | 2 cut(s) | 96, 183 |
| PfeI | GAWTC | 1 cut(s) | 22 |
| Pfl23II | CGTACG | 1 cut(s) | 410 |
| PkrI | GCNGC | 3 cut(s) | 49, 52, 463 |
| PluTI | GGCGCC | 1 cut(s) | 48 |
| Ppu21I | YACGTR | 1 cut(s) | 414 |
| Psp6I | CCWGG | 1 cut(s) | 418 |
| PspGI | CCWGG | 1 cut(s) | 418 |
| PspLI | CGTACG | 1 cut(s) | 410 |
| PspN4I | GGNNCC | 4 cut(s) | 46, 105, 288, 523 |
| PspPI | GGNCC | 2 cut(s) | 172, 281 |
| PsuI | RGATCY | 1 cut(s) | 148 |
| RsaI | GTAC | 1 cut(s) | 412 |
| RsaNI | GTAC | 1 cut(s) | 411 |
| SaqAI | TTAA | 3 cut(s) | 264, 276, 529 |
| SatI | GCNGC | 3 cut(s) | 48, 51, 462 |
| Sau3AI | GATC | 1 cut(s) | 148 |
| Sau96I | GGNCC | 2 cut(s) | 172, 281 |
| ScrFI | CCNGG | 3 cut(s) | 75, 420, 478 |
| SduI | GDGCHC | 4 cut(s) | 141, 291, 419, 526 |
| SetI | ASST | 7 cut(s) | 11, 283, 352, 412, 416, 424, 507 |
| SfaNI | GCATC | 1 cut(s) | 373 |
| SfoI | GGCGCC | 1 cut(s) | 46 |
| SinI | GGWCC | 1 cut(s) | 281 |
| SmlI | CTYRAG | 1 cut(s) | 13 |
| SmoI | CTYRAG | 1 cut(s) | 13 |
| Sse9I | AATT | 1 cut(s) | 188 |
| SsiI | CCGC | 6 cut(s) | 48, 51, 86, 107, 194, 272 |
| SspDI | GGCGCC | 1 cut(s) | 44 |
| StyD4I | CCNGG | 3 cut(s) | 73, 418, 476 |
| StyI | CCWWGG | 1 cut(s) | 517 |
| TaaI | ACNGT | 2 cut(s) | 82, 325 |
| TaiI | ACGT | 3 cut(s) | 11, 412, 416 |
| TaqI | TCGA | 1 cut(s) | 327 |
| TasI | AATT | 1 cut(s) | 188 |
| TauI | GCSGC | 2 cut(s) | 50, 53 |
| TfiI | GAWTC | 1 cut(s) | 22 |
| Tru1I | TTAA | 3 cut(s) | 264, 276, 529 |
| Tru9I | TTAA | 3 cut(s) | 264, 276, 529 |
| TseI | GCWGC | 1 cut(s) | 461 |
| TspDTI | ATGAA | 2 cut(s) | 450, 483 |
| TspGWI | ACGGA | 2 cut(s) | 104, 360 |
| VpaK11BI | GGWCC | 1 cut(s) | 281 |
| ZraI | GACGTC | 1 cut(s) | 9 |