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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 325 |
| Acc36I | ACCTGC | 1 cut(s) | 38 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 628 |
| AccI | GTMKAC | 1 cut(s) | 435 |
| AccII | CGCG | 1 cut(s) | 35 |
| AciI | CCGC | 5 cut(s) | 33, 203, 237, 303, 333 |
| AclWI | GGATC | 1 cut(s) | 734 |
| AcoI | YGGCCR | 2 cut(s) | 87, 172 |
| AcuI | CTGAAG | 1 cut(s) | 521 |
| AfaI | GTAC | 3 cut(s) | 379, 410, 564 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 628, 650 |
| AflIII | ACRYGT | 1 cut(s) | 565 |
| AgsI | TTSAA | 2 cut(s) | 109, 130 |
| AjiI | CACGTC | 1 cut(s) | 671 |
| AjnI | CCWGG | 2 cut(s) | 438, 649 |
| Alw26I | GTCTC | 1 cut(s) | 506 |
| AlwI | GGATC | 1 cut(s) | 734 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 292 |
| AoxI | GGCC | 5 cut(s) | 87, 172, 360, 578, 711 |
| ApaI | GGGCCC | 1 cut(s) | 715 |
| ApeKI | GCWGC | 1 cut(s) | 228 |
| AspA2I | CCTAGG | 1 cut(s) | 707 |
| AspS9I | GGNCC | 5 cut(s) | 578, 653, 711, 712, 737 |
| AvaII | GGWCC | 2 cut(s) | 653, 737 |
| AvrII | CCTAGG | 1 cut(s) | 707 |
| BaeGI | GKGCMC | 1 cut(s) | 715 |
| BalI | TGGCCA | 2 cut(s) | 89, 174 |
| BanII | GRGCYC | 1 cut(s) | 715 |
| BbsI | GAAGAC | 2 cut(s) | 44, 227 |
| BbvI | GCAGC | 1 cut(s) | 240 |
| BccI | CCATC | 2 cut(s) | 187, 716 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 599, 633 |
| BciT130I | CCWGG | 2 cut(s) | 440, 651 |
| BclI | TGATCA | 1 cut(s) | 690 |
| BcoDI | GTCTC | 1 cut(s) | 506 |
| BfaI | CTAG | 2 cut(s) | 225, 708 |
| BfuAI | ACCTGC | 1 cut(s) | 38 |
| BisI | GCNGC | 3 cut(s) | 203, 229, 304 |
| BlnI | CCTAGG | 1 cut(s) | 707 |
| BlsI | GCNGC | 3 cut(s) | 204, 230, 305 |
| Bme1390I | CCNGG | 2 cut(s) | 440, 651 |
| Bme18I | GGWCC | 2 cut(s) | 653, 737 |
| BmgBI | CACGTC | 1 cut(s) | 671 |
| BmgT120I | GGNCC | 5 cut(s) | 578, 653, 711, 712, 737 |
| BmiI | GGNNCC | 3 cut(s) | 579, 713, 738 |
| BmrFI | CCNGG | 2 cut(s) | 440, 651 |
| BmsI | GCATC | 1 cut(s) | 364 |
| BpiI | GAAGAC | 2 cut(s) | 44, 227 |
| BsaAI | YACGTR | 1 cut(s) | 199 |
| BsaJI | CCNNGG | 5 cut(s) | 81, 160, 449, 707, 774 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 226, 256, 625, 655 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 628, 650 |
| Bse1I | ACTGG | 1 cut(s) | 581 |
| BseBI | CCWGG | 2 cut(s) | 440, 651 |
| BseDI | CCNNGG | 5 cut(s) | 81, 160, 449, 707, 774 |
| BseGI | GGATG | 1 cut(s) | 727 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 628, 650 |
| BseNI | ACTGG | 1 cut(s) | 581 |
| BseRI | GAGGAG | 2 cut(s) | 313, 698 |
| BseSI | GKGCMC | 1 cut(s) | 715 |
| BseXI | GCAGC | 1 cut(s) | 240 |
| BseYI | CCCAGC | 1 cut(s) | 714 |
| Bsh1236I | CGCG | 1 cut(s) | 35 |
| BshFI | GGCC | 5 cut(s) | 89, 174, 362, 580, 713 |
| BsiSI | CCGG | 1 cut(s) | 719 |
| BslFI | GGGAC | 1 cut(s) | 750 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 628, 650 |
| BsmAI | GTCTC | 1 cut(s) | 506 |
| BsmFI | GGGAC | 1 cut(s) | 750 |
| BsnI | GGCC | 5 cut(s) | 89, 174, 362, 580, 713 |
| Bsp120I | GGGCCC | 1 cut(s) | 711 |
| Bsp1286I | GDGCHC | 1 cut(s) | 715 |
| Bsp1407I | TGTACA | 1 cut(s) | 562 |
| Bsp143I | GATC | 2 cut(s) | 690, 726 |
| BspACI | CCGC | 5 cut(s) | 33, 203, 237, 303, 333 |
| BspANI | GGCC | 5 cut(s) | 89, 174, 362, 580, 713 |
| BspFNI | CGCG | 1 cut(s) | 35 |
| BspLI | GGNNCC | 3 cut(s) | 579, 713, 738 |
| BspMI | ACCTGC | 1 cut(s) | 38 |
| BspPI | GGATC | 1 cut(s) | 734 |
| BsrGI | TGTACA | 1 cut(s) | 562 |
| BsrI | ACTGG | 1 cut(s) | 581 |
| BssECI | CCNNGG | 5 cut(s) | 81, 160, 449, 707, 774 |
| BssMI | GATC | 2 cut(s) | 690, 726 |
| BssNAI | GTATAC | 1 cut(s) | 436 |
| BssT1I | CCWWGG | 4 cut(s) | 160, 449, 707, 774 |
| Bst1107I | GTATAC | 1 cut(s) | 436 |
| Bst2UI | CCWGG | 2 cut(s) | 440, 651 |
| Bst4CI | ACNGT | 2 cut(s) | 65, 628 |
| Bst6I | CTCTTC | 1 cut(s) | 540 |
| BstAUI | TGTACA | 1 cut(s) | 562 |
| BstBAI | YACGTR | 1 cut(s) | 199 |
| BstC8I | GCNNGC | 4 cut(s) | 176, 288, 525, 762 |
| BstDEI | CTNAG | 1 cut(s) | 24 |
| BstF5I | GGATG | 1 cut(s) | 727 |
| BstFNI | CGCG | 1 cut(s) | 35 |
| BstKTI | GATC | 2 cut(s) | 693, 729 |
| BstMAI | GTCTC | 1 cut(s) | 506 |
| BstMBI | GATC | 2 cut(s) | 690, 726 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 74, 211, 257 |
| BstNI | CCWGG | 2 cut(s) | 440, 651 |
| BstNSI | RCATGY | 1 cut(s) | 569 |
| BstSCI | CCNGG | 2 cut(s) | 438, 649 |
| BstSLI | GKGCMC | 1 cut(s) | 715 |
| BstUI | CGCG | 1 cut(s) | 35 |
| BstV1I | GCAGC | 1 cut(s) | 240 |
| BstV2I | GAAGAC | 2 cut(s) | 44, 227 |
| BstX2I | RGATCY | 1 cut(s) | 726 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 182, 722 |
| BstYI | RGATCY | 1 cut(s) | 726 |
| BstZ17I | GTATAC | 1 cut(s) | 436 |
| BsuRI | GGCC | 5 cut(s) | 89, 174, 362, 580, 713 |
| BtrI | CACGTC | 1 cut(s) | 671 |
| BtsCI | GGATG | 1 cut(s) | 727 |
| BtsI | GCAGTG | 1 cut(s) | 534 |
| BtsIMutI | CAGTG | 4 cut(s) | 70, 269, 534, 588 |
| BveI | ACCTGC | 1 cut(s) | 38 |
| Cac8I | GCNNGC | 4 cut(s) | 176, 288, 525, 762 |
| CaiI | CAGNNNCTG | 1 cut(s) | 292 |
| Cfr13I | GGNCC | 5 cut(s) | 578, 653, 711, 712, 737 |
| CseI | GACGC | 1 cut(s) | 24 |
| Csp6I | GTAC | 3 cut(s) | 378, 409, 563 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 66, 101 |
| CviAII | CATG | 5 cut(s) | 91, 330, 373, 566, 757 |
| CviQI | GTAC | 3 cut(s) | 378, 409, 563 |
| DdeI | CTNAG | 1 cut(s) | 24 |
| DpnI | GATC | 2 cut(s) | 692, 728 |
| DpnII | GATC | 2 cut(s) | 690, 726 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 325 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 325 |
| EaeI | YGGCCR | 2 cut(s) | 87, 172 |
| Eam1104I | CTCTTC | 1 cut(s) | 540 |
| EarI | CTCTTC | 1 cut(s) | 540 |
| Eco130I | CCWWGG | 4 cut(s) | 160, 449, 707, 774 |
| Eco147I | AGGCCT | 1 cut(s) | 362 |
| Eco24I | GRGCYC | 1 cut(s) | 715 |
| Eco47I | GGWCC | 2 cut(s) | 653, 737 |
| Eco57I | CTGAAG | 1 cut(s) | 521 |
| EcoO109I | RGGNCCY | 2 cut(s) | 711, 737 |
| EcoRII | CCWGG | 2 cut(s) | 438, 649 |
| EcoT14I | CCWWGG | 4 cut(s) | 160, 449, 707, 774 |
| EcoT38I | GRGCYC | 1 cut(s) | 715 |
| ErhI | CCWWGG | 4 cut(s) | 160, 449, 707, 774 |
| FaeI | CATG | 5 cut(s) | 94, 333, 376, 569, 760 |
| FaqI | GGGAC | 1 cut(s) | 750 |
| FatI | CATG | 5 cut(s) | 90, 329, 372, 565, 756 |
| FbaI | TGATCA | 1 cut(s) | 690 |
| FblI | GTMKAC | 1 cut(s) | 435 |
| Fnu4HI | GCNGC | 3 cut(s) | 203, 229, 304 |
| FokI | GGATG | 1 cut(s) | 734 |
| FriOI | GRGCYC | 1 cut(s) | 715 |
| Fsp4HI | GCNGC | 3 cut(s) | 203, 229, 304 |
| FspBI | CTAG | 2 cut(s) | 225, 708 |
| GluI | GCNGC | 3 cut(s) | 203, 229, 304 |
| GsaI | CCCAGC | 1 cut(s) | 718 |
| HaeIII | GGCC | 5 cut(s) | 89, 174, 362, 580, 713 |
| HapII | CCGG | 1 cut(s) | 719 |
| HgaI | GACGC | 1 cut(s) | 24 |
| Hin1II | CATG | 5 cut(s) | 94, 333, 376, 569, 760 |
| HinfI | GANTC | 2 cut(s) | 145, 532 |
| HpaII | CCGG | 1 cut(s) | 719 |
| Hpy166II | GTNNAC | 1 cut(s) | 436 |
| Hpy188I | TCNGA | 1 cut(s) | 40 |
| Hpy188III | TCNNGA | 2 cut(s) | 13, 656 |
| Hpy8I | GTNNAC | 1 cut(s) | 436 |
| Hpy99I | CGWCG | 1 cut(s) | 422 |
| HpyCH4III | ACNGT | 2 cut(s) | 65, 628 |
| HpyCH4IV | ACGT | 4 cut(s) | 198, 383, 558, 670 |
| HpyCH4V | TGCA | 2 cut(s) | 94, 610 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 74, 211, 257 |
| HpyF3I | CTNAG | 1 cut(s) | 24 |
| HpySE526I | ACGT | 4 cut(s) | 198, 383, 558, 670 |
| Hsp92II | CATG | 5 cut(s) | 94, 333, 376, 569, 760 |
| Ksp22I | TGATCA | 1 cut(s) | 690 |
| Kzo9I | GATC | 2 cut(s) | 690, 726 |
| Lsp1109I | GCAGC | 1 cut(s) | 240 |
| LweI | GCATC | 1 cut(s) | 364 |
| MaeI | CTAG | 2 cut(s) | 225, 708 |
| MaeII | ACGT | 4 cut(s) | 198, 383, 558, 670 |
| MaeIII | GTNAC | 3 cut(s) | 10, 529, 583 |
| MalI | GATC | 2 cut(s) | 692, 728 |
| MboI | GATC | 2 cut(s) | 690, 726 |
| MboII | GAAGA | 3 cut(s) | 44, 232, 527 |
| MflI | RGATCY | 1 cut(s) | 726 |
| MhlI | GDGCHC | 1 cut(s) | 715 |
| MlsI | TGGCCA | 2 cut(s) | 89, 174 |
| MluCI | AATT | 2 cut(s) | 104, 443 |
| MluNI | TGGCCA | 2 cut(s) | 89, 174 |
| MlyI | GAGTC | 1 cut(s) | 526 |
| MmeI | TCCRAC | 1 cut(s) | 411 |
| Mox20I | TGGCCA | 2 cut(s) | 89, 174 |
| MscI | TGGCCA | 2 cut(s) | 89, 174 |
| MseI | TTAA | 2 cut(s) | 489, 808 |
| Msp20I | TGGCCA | 2 cut(s) | 89, 174 |
| MspA1I | CMGCKG | 1 cut(s) | 303 |
| MspI | CCGG | 1 cut(s) | 719 |
| MspR9I | CCNGG | 2 cut(s) | 440, 651 |
| MvaI | CCWGG | 2 cut(s) | 440, 651 |
| MvnI | CGCG | 1 cut(s) | 35 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 74, 211, 257 |
| NdeII | GATC | 2 cut(s) | 690, 726 |
| NlaIII | CATG | 5 cut(s) | 94, 333, 376, 569, 760 |
| NlaIV | GGNNCC | 3 cut(s) | 579, 713, 738 |
| NmuCI | GTSAC | 3 cut(s) | 10, 529, 583 |
| NspI | RCATGY | 1 cut(s) | 569 |
| PceI | AGGCCT | 1 cut(s) | 362 |
| PciI | ACATGT | 1 cut(s) | 565 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 408 |
| PfeI | GAWTC | 1 cut(s) | 145 |
| PflFI | GACNNNGTC | 1 cut(s) | 34 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 628 |
| PkrI | GCNGC | 3 cut(s) | 204, 230, 305 |
| PleI | GAGTC | 1 cut(s) | 526 |
| PpsI | GAGTC | 1 cut(s) | 526 |
| Ppu21I | YACGTR | 1 cut(s) | 199 |
| PpuMI | RGGWCCY | 1 cut(s) | 737 |
| PscI | ACATGT | 1 cut(s) | 565 |
| Psp5II | RGGWCCY | 1 cut(s) | 737 |
| Psp6I | CCWGG | 2 cut(s) | 438, 649 |
| PspFI | CCCAGC | 1 cut(s) | 714 |
| PspGI | CCWGG | 2 cut(s) | 438, 649 |
| PspN4I | GGNNCC | 3 cut(s) | 579, 713, 738 |
| PspOMI | GGGCCC | 1 cut(s) | 711 |
| PspPI | GGNCC | 5 cut(s) | 578, 653, 711, 712, 737 |
| PspPPI | RGGWCCY | 1 cut(s) | 737 |
| PstNI | CAGNNNCTG | 1 cut(s) | 292 |
| PsuI | RGATCY | 1 cut(s) | 726 |
| PsyI | GACNNNGTC | 1 cut(s) | 34 |
| RsaI | GTAC | 3 cut(s) | 379, 410, 564 |
| RsaNI | GTAC | 3 cut(s) | 378, 409, 563 |
| SaqAI | TTAA | 2 cut(s) | 489, 808 |
| SatI | GCNGC | 3 cut(s) | 203, 229, 304 |
| Sau3AI | GATC | 2 cut(s) | 690, 726 |
| Sau96I | GGNCC | 5 cut(s) | 578, 653, 711, 712, 737 |
| SchI | GAGTC | 1 cut(s) | 526 |
| ScrFI | CCNGG | 2 cut(s) | 440, 651 |
| SduI | GDGCHC | 1 cut(s) | 715 |
| SfaNI | GCATC | 1 cut(s) | 364 |
| SinI | GGWCC | 2 cut(s) | 653, 737 |
| Sse9I | AATT | 2 cut(s) | 104, 443 |
| SseBI | AGGCCT | 1 cut(s) | 362 |
| SsiI | CCGC | 5 cut(s) | 33, 203, 237, 303, 333 |
| SspMI | CTAG | 2 cut(s) | 225, 708 |
| StuI | AGGCCT | 1 cut(s) | 362 |
| StyD4I | CCNGG | 2 cut(s) | 438, 649 |
| StyI | CCWWGG | 4 cut(s) | 160, 449, 707, 774 |
| TaaI | ACNGT | 2 cut(s) | 65, 628 |
| TaiI | ACGT | 4 cut(s) | 201, 386, 561, 673 |
| TaqI | TCGA | 1 cut(s) | 365 |
| TasI | AATT | 2 cut(s) | 104, 443 |
| TatI | WGTACW | 1 cut(s) | 562 |
| TauI | GCSGC | 2 cut(s) | 205, 306 |
| TfiI | GAWTC | 1 cut(s) | 145 |
| Tru1I | TTAA | 2 cut(s) | 489, 808 |
| Tru9I | TTAA | 2 cut(s) | 489, 808 |
| TscAI | CASTG | 4 cut(s) | 70, 276, 534, 588 |
| TseFI | GTSAC | 3 cut(s) | 10, 529, 583 |
| TseI | GCWGC | 1 cut(s) | 228 |
| Tsp45I | GTSAC | 3 cut(s) | 10, 529, 583 |
| TspRI | CASTG | 4 cut(s) | 70, 276, 534, 588 |
| Tth111I | GACNNNGTC | 1 cut(s) | 34 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 628 |
| VpaK11BI | GGWCC | 2 cut(s) | 653, 737 |
| XceI | RCATGY | 1 cut(s) | 569 |
| XmaJI | CCTAGG | 1 cut(s) | 707 |
| XmiI | GTMKAC | 1 cut(s) | 435 |
| XspI | CTAG | 2 cut(s) | 225, 708 |