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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 224 |
| AccBSI | CCGCTC | 1 cut(s) | 1057 |
| AccII | CGCG | 3 cut(s) | 352, 354, 774 |
| AccIII | TCCGGA | 1 cut(s) | 569 |
| AclWI | GGATC | 3 cut(s) | 864, 877, 1197 |
| AcoI | YGGCCR | 1 cut(s) | 896 |
| AcuI | CTGAAG | 4 cut(s) | 314, 394, 1017, 1239 |
| AfaI | GTAC | 1 cut(s) | 921 |
| AfeI | AGCGCT | 1 cut(s) | 1015 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 592, 842 |
| AflIII | ACRYGT | 1 cut(s) | 421 |
| AgsI | TTSAA | 6 cut(s) | 75, 162, 654, 833, 854, 1131 |
| AjnI | CCWGG | 2 cut(s) | 484, 1172 |
| AleI | CACNNNNGTG | 1 cut(s) | 525 |
| AluBI | AGCT | 3 cut(s) | 680, 861, 975 |
| AluI | AGCT | 3 cut(s) | 680, 861, 975 |
| Alw21I | GWGCWC | 2 cut(s) | 55, 142 |
| Alw26I | GTCTC | 1 cut(s) | 237 |
| AlwI | GGATC | 3 cut(s) | 864, 877, 1197 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 978, 1169 |
| Aor13HI | TCCGGA | 1 cut(s) | 569 |
| Aor51HI | AGCGCT | 1 cut(s) | 1015 |
| AoxI | GGCC | 5 cut(s) | 487, 763, 896, 1063, 1084 |
| ApaI | GGGCCC | 1 cut(s) | 767 |
| ApeKI | GCWGC | 3 cut(s) | 952, 972, 1030 |
| AspLEI | GCGC | 4 cut(s) | 225, 354, 1016, 1241 |
| AspS9I | GGNCC | 2 cut(s) | 763, 764 |
| AsuHPI | GGTGA | 5 cut(s) | 406, 438, 509, 698, 770 |
| AsuNHI | GCTAGC | 1 cut(s) | 783 |
| BaeGI | GKGCMC | 1 cut(s) | 767 |
| BalI | TGGCCA | 1 cut(s) | 898 |
| BamHI | GGATCC | 1 cut(s) | 869 |
| BanII | GRGCYC | 1 cut(s) | 767 |
| Bbv12I | GWGCWC | 2 cut(s) | 55, 142 |
| BbvI | GCAGC | 3 cut(s) | 964, 984, 1042 |
| BccI | CCATC | 2 cut(s) | 911, 1137 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 119, 153 |
| BciT130I | CCWGG | 2 cut(s) | 486, 1174 |
| BcoDI | GTCTC | 1 cut(s) | 237 |
| BfaI | CTAG | 7 cut(s) | 24, 111, 277, 693, 727, 784, 949 |
| BfmI | CTRYAG | 1 cut(s) | 1243 |
| BfoI | RGCGCY | 2 cut(s) | 1017, 1242 |
| BisI | GCNGC | 7 cut(s) | 643, 772, 927, 953, 973, 1028, 1031 |
| BlsI | GCNGC | 7 cut(s) | 644, 773, 928, 954, 974, 1029, 1032 |
| Bme1390I | CCNGG | 2 cut(s) | 486, 1174 |
| BmgT120I | GGNCC | 2 cut(s) | 763, 764 |
| BmiI | GGNNCC | 2 cut(s) | 765, 871 |
| BmrFI | CCNGG | 2 cut(s) | 486, 1174 |
| BmsI | GCATC | 1 cut(s) | 1088 |
| BmtI | GCTAGC | 1 cut(s) | 787 |
| BsaAI | YACGTR | 1 cut(s) | 923 |
| BsaJI | CCNNGG | 3 cut(s) | 204, 586, 884 |
| BsaWI | WCCGGW | 1 cut(s) | 569 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1190, 1220 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 592, 842 |
| Bse1I | ACTGG | 3 cut(s) | 797, 907, 1205 |
| BseAI | TCCGGA | 1 cut(s) | 569 |
| BseBI | CCWGG | 2 cut(s) | 486, 1174 |
| BseDI | CCNNGG | 3 cut(s) | 204, 586, 884 |
| BseGI | GGATG | 2 cut(s) | 903, 1107 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 592, 842 |
| BseMII | CTCAG | 1 cut(s) | 451 |
| BseNI | ACTGG | 3 cut(s) | 797, 907, 1205 |
| BseRI | GAGGAG | 1 cut(s) | 47 |
| BseSI | GKGCMC | 1 cut(s) | 767 |
| BseXI | GCAGC | 3 cut(s) | 964, 984, 1042 |
| BsgI | GTGCAG | 1 cut(s) | 182 |
| Bsh1236I | CGCG | 3 cut(s) | 352, 354, 774 |
| Bsh1285I | CGRYCG | 1 cut(s) | 454 |
| BshFI | GGCC | 5 cut(s) | 489, 765, 898, 1065, 1086 |
| BsiEI | CGRYCG | 1 cut(s) | 454 |
| BsiHKAI | GWGCWC | 2 cut(s) | 55, 142 |
| BsiSI | CCGG | 1 cut(s) | 570 |
| BslFI | GGGAC | 1 cut(s) | 392 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 592, 842 |
| BsmAI | GTCTC | 1 cut(s) | 237 |
| BsmFI | GGGAC | 1 cut(s) | 392 |
| BsmI | GAATGC | 1 cut(s) | 758 |
| BsnI | GGCC | 5 cut(s) | 489, 765, 898, 1065, 1086 |
| Bsp120I | GGGCCC | 1 cut(s) | 763 |
| Bsp1286I | GDGCHC | 3 cut(s) | 55, 142, 767 |
| Bsp13I | TCCGGA | 1 cut(s) | 569 |
| Bsp143I | GATC | 4 cut(s) | 695, 736, 869, 1189 |
| BspANI | GGCC | 5 cut(s) | 489, 765, 898, 1065, 1086 |
| BspCNI | CTCAG | 1 cut(s) | 452 |
| BspEI | TCCGGA | 1 cut(s) | 569 |
| BspFNI | CGCG | 3 cut(s) | 352, 354, 774 |
| BspLI | GGNNCC | 2 cut(s) | 765, 871 |
| BspOI | GCTAGC | 1 cut(s) | 787 |
| BspPI | GGATC | 3 cut(s) | 864, 877, 1197 |
| BsrBI | CCGCTC | 1 cut(s) | 1057 |
| BsrI | ACTGG | 3 cut(s) | 797, 907, 1205 |
| BssECI | CCNNGG | 3 cut(s) | 204, 586, 884 |
| BssMI | GATC | 4 cut(s) | 695, 736, 869, 1189 |
| BssT1I | CCWWGG | 1 cut(s) | 884 |
| Bst2UI | CCWGG | 2 cut(s) | 486, 1174 |
| Bst4CI | ACNGT | 8 cut(s) | 208, 248, 455, 515, 527, 745, 980, 1247 |
| Bst6I | CTCTTC | 3 cut(s) | 433, 993, 1258 |
| BstBAI | YACGTR | 1 cut(s) | 923 |
| BstC8I | GCNNGC | 9 cut(s) | 213, 217, 221, 491, 495, 785, 789, 900, 1012 |
| BstDEI | CTNAG | 1 cut(s) | 460 |
| BstDSI | CCRYGG | 1 cut(s) | 204 |
| BstF5I | GGATG | 2 cut(s) | 903, 1107 |
| BstFNI | CGCG | 3 cut(s) | 352, 354, 774 |
| BstH2I | RGCGCY | 2 cut(s) | 1017, 1242 |
| BstHHI | GCGC | 4 cut(s) | 225, 354, 1016, 1241 |
| BstKTI | GATC | 4 cut(s) | 698, 739, 872, 1192 |
| BstMAI | GTCTC | 1 cut(s) | 237 |
| BstMBI | GATC | 4 cut(s) | 695, 736, 869, 1189 |
| BstMCI | CGRYCG | 1 cut(s) | 454 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 762, 771, 780, 895, 969, 981 |
| BstNI | CCWGG | 2 cut(s) | 486, 1174 |
| BstNSI | RCATGY | 2 cut(s) | 425, 717 |
| BstSCI | CCNGG | 2 cut(s) | 484, 1172 |
| BstSFI | CTRYAG | 1 cut(s) | 1243 |
| BstSLI | GKGCMC | 1 cut(s) | 767 |
| BstUI | CGCG | 3 cut(s) | 352, 354, 774 |
| BstV1I | GCAGC | 3 cut(s) | 964, 984, 1042 |
| BstX2I | RGATCY | 1 cut(s) | 869 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 211, 527 |
| BstYI | RGATCY | 1 cut(s) | 869 |
| BsuRI | GGCC | 5 cut(s) | 489, 765, 898, 1065, 1086 |
| BtgI | CCRYGG | 1 cut(s) | 204 |
| BtgZI | GCGATG | 2 cut(s) | 612, 1156 |
| BtsCI | GGATG | 2 cut(s) | 903, 1107 |
| BtsIMutI | CAGTG | 3 cut(s) | 804, 942, 1252 |
| Cac8I | GCNNGC | 9 cut(s) | 213, 217, 221, 491, 495, 785, 789, 900, 1012 |
| CaiI | CAGNNNCTG | 2 cut(s) | 978, 1169 |
| CfoI | GCGC | 4 cut(s) | 225, 354, 1016, 1241 |
| Cfr13I | GGNCC | 2 cut(s) | 763, 764 |
| Csp6I | GTAC | 1 cut(s) | 920 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 53, 88, 140, 175 |
| CviAII | CATG | 4 cut(s) | 422, 608, 714, 768 |
| CviQI | GTAC | 1 cut(s) | 920 |
| DdeI | CTNAG | 1 cut(s) | 460 |
| DpnI | GATC | 4 cut(s) | 697, 738, 871, 1191 |
| DpnII | GATC | 4 cut(s) | 695, 736, 869, 1189 |
| EaeI | YGGCCR | 1 cut(s) | 896 |
| Eam1104I | CTCTTC | 3 cut(s) | 433, 993, 1258 |
| EarI | CTCTTC | 3 cut(s) | 433, 993, 1258 |
| Eco130I | CCWWGG | 1 cut(s) | 884 |
| Eco147I | AGGCCT | 1 cut(s) | 1086 |
| Eco24I | GRGCYC | 1 cut(s) | 767 |
| Eco47III | AGCGCT | 1 cut(s) | 1015 |
| Eco57I | CTGAAG | 4 cut(s) | 314, 394, 1017, 1239 |
| EcoO109I | RGGNCCY | 1 cut(s) | 763 |
| EcoRII | CCWGG | 2 cut(s) | 484, 1172 |
| EcoT14I | CCWWGG | 1 cut(s) | 884 |
| EcoT38I | GRGCYC | 1 cut(s) | 767 |
| ErhI | CCWWGG | 1 cut(s) | 884 |
| FaeI | CATG | 4 cut(s) | 425, 611, 717, 771 |
| FaiI | YATR | 9 cut(s) | 322, 423, 609, 684, 715, 769, 816, 1160, 1198 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 533, 565 |
| FaqI | GGGAC | 1 cut(s) | 392 |
| FatI | CATG | 4 cut(s) | 421, 607, 713, 767 |
| FauI | CCCGC | 2 cut(s) | 977, 1091 |
| Fnu4HI | GCNGC | 7 cut(s) | 643, 772, 927, 953, 973, 1028, 1031 |
| FokI | GGATG | 2 cut(s) | 890, 1094 |
| FriOI | GRGCYC | 1 cut(s) | 767 |
| Fsp4HI | GCNGC | 7 cut(s) | 643, 772, 927, 953, 973, 1028, 1031 |
| FspBI | CTAG | 7 cut(s) | 24, 111, 277, 693, 727, 784, 949 |
| FspI | TGCGCA | 1 cut(s) | 224 |
| GlaI | GCGC | 4 cut(s) | 224, 353, 1015, 1240 |
| GluI | GCNGC | 7 cut(s) | 643, 772, 927, 953, 973, 1028, 1031 |
| HaeII | RGCGCY | 2 cut(s) | 1017, 1242 |
| HaeIII | GGCC | 5 cut(s) | 489, 765, 898, 1065, 1086 |
| HapII | CCGG | 1 cut(s) | 570 |
| HhaI | GCGC | 4 cut(s) | 225, 354, 1016, 1241 |
| Hin1II | CATG | 4 cut(s) | 425, 611, 717, 771 |
| Hin6I | GCGC | 4 cut(s) | 223, 352, 1014, 1239 |
| HinP1I | GCGC | 4 cut(s) | 223, 352, 1014, 1239 |
| HincII | GTYRAC | 1 cut(s) | 427 |
| HindII | GTYRAC | 1 cut(s) | 427 |
| HindIII | AAGCTT | 1 cut(s) | 859 |
| HinfI | GANTC | 8 cut(s) | 32, 119, 386, 394, 443, 623, 703, 1250 |
| HpaII | CCGG | 1 cut(s) | 570 |
| HphI | GGTGA | 5 cut(s) | 406, 438, 509, 698, 770 |
| Hpy166II | GTNNAC | 5 cut(s) | 64, 151, 302, 427, 598 |
| Hpy188I | TCNGA | 4 cut(s) | 374, 469, 587, 749 |
| Hpy188III | TCNNGA | 9 cut(s) | 15, 102, 159, 570, 599, 693, 1164, 1187, 1214 |
| Hpy8I | GTNNAC | 5 cut(s) | 64, 151, 302, 427, 598 |
| Hpy99I | CGWCG | 2 cut(s) | 195, 1055 |
| HpyAV | CCTTC | 3 cut(s) | 338, 567, 1085 |
| HpyCH4III | ACNGT | 8 cut(s) | 208, 248, 455, 515, 527, 745, 980, 1247 |
| HpyCH4IV | ACGT | 2 cut(s) | 922, 1122 |
| HpyCH4V | TGCA | 3 cut(s) | 199, 253, 717 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 762, 771, 780, 895, 969, 981 |
| HpyF3I | CTNAG | 1 cut(s) | 460 |
| HpySE526I | ACGT | 2 cut(s) | 922, 1122 |
| Hsp92II | CATG | 4 cut(s) | 425, 611, 717, 771 |
| HspAI | GCGC | 4 cut(s) | 223, 352, 1014, 1239 |
| Kpn2I | TCCGGA | 1 cut(s) | 569 |
| Kzo9I | GATC | 4 cut(s) | 695, 736, 869, 1189 |
| LmnI | GCTCC | 1 cut(s) | 576 |
| Lsp1109I | GCAGC | 3 cut(s) | 964, 984, 1042 |
| LweI | GCATC | 1 cut(s) | 1088 |
| MaeI | CTAG | 7 cut(s) | 24, 111, 277, 693, 727, 784, 949 |
| MaeII | ACGT | 2 cut(s) | 922, 1122 |
| MaeIII | GTNAC | 5 cut(s) | 333, 383, 455, 515, 1247 |
| MalI | GATC | 4 cut(s) | 697, 738, 871, 1191 |
| MbiI | CCGCTC | 1 cut(s) | 1057 |
| MboI | GATC | 4 cut(s) | 695, 736, 869, 1189 |
| MflI | RGATCY | 1 cut(s) | 869 |
| MhlI | GDGCHC | 3 cut(s) | 55, 142, 767 |
| MlsI | TGGCCA | 1 cut(s) | 898 |
| MluCI | AATT | 2 cut(s) | 811, 828 |
| MluNI | TGGCCA | 1 cut(s) | 898 |
| MlyI | GAGTC | 2 cut(s) | 380, 1244 |
| MmeI | TCCRAC | 2 cut(s) | 727, 1135 |
| Mox20I | TGGCCA | 1 cut(s) | 898 |
| MroI | TCCGGA | 1 cut(s) | 569 |
| MscI | TGGCCA | 1 cut(s) | 898 |
| MslI | CAYNNNNRTG | 1 cut(s) | 525 |
| Msp20I | TGGCCA | 1 cut(s) | 898 |
| MspA1I | CMGCKG | 2 cut(s) | 645, 995 |
| MspI | CCGG | 1 cut(s) | 570 |
| MspR9I | CCNGG | 2 cut(s) | 486, 1174 |
| Mva1269I | GAATGC | 1 cut(s) | 758 |
| MvaI | CCWGG | 2 cut(s) | 486, 1174 |
| MvnI | CGCG | 3 cut(s) | 352, 354, 774 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 762, 771, 780, 895, 969, 981 |
| NdeII | GATC | 4 cut(s) | 695, 736, 869, 1189 |
| NheI | GCTAGC | 1 cut(s) | 783 |
| NlaIII | CATG | 4 cut(s) | 425, 611, 717, 771 |
| NlaIV | GGNNCC | 2 cut(s) | 765, 871 |
| NmuCI | GTSAC | 3 cut(s) | 383, 515, 1247 |
| NsbI | TGCGCA | 1 cut(s) | 224 |
| NspI | RCATGY | 2 cut(s) | 425, 717 |
| OliI | CACNNNNGTG | 1 cut(s) | 525 |
| PceI | AGGCCT | 1 cut(s) | 1086 |
| PciI | ACATGT | 1 cut(s) | 421 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1132 |
| PctI | GAATGC | 1 cut(s) | 758 |
| PfeI | GAWTC | 6 cut(s) | 32, 119, 394, 443, 623, 703 |
| PkrI | GCNGC | 7 cut(s) | 644, 773, 928, 954, 974, 1029, 1032 |
| PleI | GAGTC | 2 cut(s) | 380, 1244 |
| PpsI | GAGTC | 2 cut(s) | 380, 1244 |
| Ppu21I | YACGTR | 1 cut(s) | 923 |
| PscI | ACATGT | 1 cut(s) | 421 |
| Psp6I | CCWGG | 2 cut(s) | 484, 1172 |
| PspGI | CCWGG | 2 cut(s) | 484, 1172 |
| PspN4I | GGNNCC | 2 cut(s) | 765, 871 |
| PspOMI | GGGCCC | 1 cut(s) | 763 |
| PspPI | GGNCC | 2 cut(s) | 763, 764 |
| PstNI | CAGNNNCTG | 2 cut(s) | 978, 1169 |
| PsuI | RGATCY | 1 cut(s) | 869 |
| RsaI | GTAC | 1 cut(s) | 921 |
| RsaNI | GTAC | 1 cut(s) | 920 |
| RseI | CAYNNNNRTG | 1 cut(s) | 525 |
| SatI | GCNGC | 7 cut(s) | 643, 772, 927, 953, 973, 1028, 1031 |
| Sau3AI | GATC | 4 cut(s) | 695, 736, 869, 1189 |
| Sau96I | GGNCC | 2 cut(s) | 763, 764 |
| SchI | GAGTC | 2 cut(s) | 380, 1244 |
| ScrFI | CCNGG | 2 cut(s) | 486, 1174 |
| SduI | GDGCHC | 3 cut(s) | 55, 142, 767 |
| SfaNI | GCATC | 1 cut(s) | 1088 |
| SfcI | CTRYAG | 1 cut(s) | 1243 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 525 |
| Sse9I | AATT | 2 cut(s) | 811, 828 |
| SseBI | AGGCCT | 1 cut(s) | 1086 |
| SspI | AATATT | 2 cut(s) | 617, 959 |
| SspMI | CTAG | 7 cut(s) | 24, 111, 277, 693, 727, 784, 949 |
| StuI | AGGCCT | 1 cut(s) | 1086 |
| StyD4I | CCNGG | 2 cut(s) | 484, 1172 |
| StyI | CCWWGG | 1 cut(s) | 884 |
| TaaI | ACNGT | 8 cut(s) | 208, 248, 455, 515, 527, 745, 980, 1247 |
| TaiI | ACGT | 2 cut(s) | 925, 1125 |
| TaqI | TCGA | 3 cut(s) | 1053, 1089, 1192 |
| TasI | AATT | 2 cut(s) | 811, 828 |
| TauI | GCSGC | 4 cut(s) | 645, 774, 929, 1030 |
| TfiI | GAWTC | 6 cut(s) | 32, 119, 394, 443, 623, 703 |
| TscAI | CASTG | 3 cut(s) | 804, 942, 1252 |
| TseFI | GTSAC | 3 cut(s) | 383, 515, 1247 |
| TseI | GCWGC | 3 cut(s) | 952, 972, 1030 |
| Tsp45I | GTSAC | 3 cut(s) | 383, 515, 1247 |
| TspDTI | ATGAA | 1 cut(s) | 1096 |
| TspRI | CASTG | 3 cut(s) | 804, 942, 1252 |
| XbaI | TCTAGA | 1 cut(s) | 692 |
| XceI | RCATGY | 2 cut(s) | 425, 717 |
| XspI | CTAG | 7 cut(s) | 24, 111, 277, 693, 727, 784, 949 |