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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 91 |
| AccBSI | CCGCTC | 1 cut(s) | 457 |
| AccII | CGCG | 2 cut(s) | 393, 549 |
| AclWI | GGATC | 1 cut(s) | 371 |
| AcoI | YGGCCR | 2 cut(s) | 348, 362 |
| AfaI | GTAC | 2 cut(s) | 187, 321 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 484 |
| AgsI | TTSAA | 3 cut(s) | 311, 482, 491 |
| AloI | GAACNNNNNNTCC | 1 cut(s) | 29 |
| AluBI | AGCT | 5 cut(s) | 56, 172, 357, 516, 576 |
| AluI | AGCT | 5 cut(s) | 56, 172, 357, 516, 576 |
| Alw21I | GWGCWC | 1 cut(s) | 508 |
| Alw26I | GTCTC | 3 cut(s) | 14, 104, 283 |
| AlwI | GGATC | 1 cut(s) | 371 |
| AoxI | GGCC | 5 cut(s) | 72, 129, 348, 362, 527 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 306 |
| AspLEI | GCGC | 1 cut(s) | 115 |
| AspS9I | GGNCC | 1 cut(s) | 73 |
| AsuC2I | CCSGG | 1 cut(s) | 413 |
| AsuHPI | GGTGA | 1 cut(s) | 418 |
| AsuNHI | GCTAGC | 1 cut(s) | 172 |
| BanII | GRGCYC | 2 cut(s) | 42, 195 |
| BauI | CACGAG | 3 cut(s) | 152, 352, 579 |
| Bbv12I | GWGCWC | 1 cut(s) | 508 |
| BccI | CCATC | 5 cut(s) | 64, 229, 396, 519, 605 |
| BcnI | CCSGG | 1 cut(s) | 413 |
| BcoDI | GTCTC | 3 cut(s) | 14, 104, 283 |
| BfaI | CTAG | 2 cut(s) | 53, 173 |
| BfoI | RGCGCY | 1 cut(s) | 116 |
| BfuAI | ACCTGC | 1 cut(s) | 91 |
| BglII | AGATCT | 1 cut(s) | 49 |
| BisI | GCNGC | 5 cut(s) | 212, 220, 348, 447, 458 |
| BlsI | GCNGC | 5 cut(s) | 213, 221, 349, 448, 459 |
| Bme1390I | CCNGG | 1 cut(s) | 413 |
| BmgT120I | GGNCC | 1 cut(s) | 73 |
| BmiI | GGNNCC | 1 cut(s) | 192 |
| BmrFI | CCNGG | 1 cut(s) | 413 |
| BmsI | GCATC | 2 cut(s) | 280, 554 |
| BmtI | GCTAGC | 1 cut(s) | 176 |
| BpuEI | CTTGAG | 1 cut(s) | 502 |
| BpuMI | CCSGG | 1 cut(s) | 413 |
| BsaAI | YACGTR | 1 cut(s) | 323 |
| BsaI | GGTCTC | 1 cut(s) | 104 |
| BsaJI | CCNNGG | 2 cut(s) | 132, 499 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 484 |
| Bse118I | RCCGGY | 2 cut(s) | 326, 360 |
| BseDI | CCNNGG | 2 cut(s) | 132, 499 |
| BseGI | GGATG | 2 cut(s) | 75, 433 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 484 |
| BseRI | GAGGAG | 2 cut(s) | 437, 446 |
| BseX3I | CGGCCG | 1 cut(s) | 362 |
| Bsh1236I | CGCG | 2 cut(s) | 393, 549 |
| Bsh1285I | CGRYCG | 2 cut(s) | 233, 365 |
| BshFI | GGCC | 5 cut(s) | 74, 131, 350, 364, 529 |
| BsiEI | CGRYCG | 2 cut(s) | 233, 365 |
| BsiHKAI | GWGCWC | 1 cut(s) | 508 |
| BsiSI | CCGG | 3 cut(s) | 327, 361, 412 |
| BsiWI | CGTACG | 1 cut(s) | 319 |
| BslFI | GGGAC | 1 cut(s) | 428 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 484 |
| BsmAI | GTCTC | 3 cut(s) | 14, 104, 283 |
| BsmBI | CGTCTC | 1 cut(s) | 283 |
| BsmFI | GGGAC | 1 cut(s) | 428 |
| BsmI | GAATGC | 1 cut(s) | 308 |
| BsnI | GGCC | 5 cut(s) | 74, 131, 350, 364, 529 |
| Bso31I | GGTCTC | 1 cut(s) | 104 |
| Bsp1286I | GDGCHC | 3 cut(s) | 42, 195, 508 |
| Bsp143I | GATC | 3 cut(s) | 49, 337, 376 |
| Bsp68I | TCGCGA | 1 cut(s) | 549 |
| BspANI | GGCC | 5 cut(s) | 74, 131, 350, 364, 529 |
| BspFNI | CGCG | 2 cut(s) | 393, 549 |
| BspLI | GGNNCC | 1 cut(s) | 192 |
| BspMI | ACCTGC | 1 cut(s) | 91 |
| BspOI | GCTAGC | 1 cut(s) | 176 |
| BspPI | GGATC | 1 cut(s) | 371 |
| BspTNI | GGTCTC | 1 cut(s) | 104 |
| BsrBI | CCGCTC | 1 cut(s) | 457 |
| BsrFI | RCCGGY | 2 cut(s) | 326, 360 |
| BssAI | RCCGGY | 2 cut(s) | 326, 360 |
| BssECI | CCNNGG | 2 cut(s) | 132, 499 |
| BssMI | GATC | 3 cut(s) | 49, 337, 376 |
| BssSI | CACGAG | 3 cut(s) | 152, 352, 579 |
| BssT1I | CCWWGG | 2 cut(s) | 132, 499 |
| Bst2BI | CACGAG | 3 cut(s) | 152, 352, 579 |
| Bst4CI | ACNGT | 3 cut(s) | 231, 487, 510 |
| BstBAI | YACGTR | 1 cut(s) | 323 |
| BstC8I | GCNNGC | 1 cut(s) | 174 |
| BstDEI | CTNAG | 2 cut(s) | 278, 536 |
| BstF5I | GGATG | 2 cut(s) | 75, 433 |
| BstFNI | CGCG | 2 cut(s) | 393, 549 |
| BstH2I | RGCGCY | 1 cut(s) | 116 |
| BstHHI | GCGC | 1 cut(s) | 115 |
| BstKTI | GATC | 3 cut(s) | 52, 340, 379 |
| BstMAI | GTCTC | 3 cut(s) | 14, 104, 283 |
| BstMBI | GATC | 3 cut(s) | 49, 337, 376 |
| BstMCI | CGRYCG | 2 cut(s) | 233, 365 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 546, 573 |
| BstSCI | CCNGG | 1 cut(s) | 411 |
| BstUI | CGCG | 2 cut(s) | 393, 549 |
| BstX2I | RGATCY | 1 cut(s) | 49 |
| BstYI | RGATCY | 1 cut(s) | 49 |
| BstZI | CGGCCG | 1 cut(s) | 362 |
| BsuRI | GGCC | 5 cut(s) | 74, 131, 350, 364, 529 |
| BtgZI | GCGATG | 1 cut(s) | 374 |
| BtsCI | GGATG | 2 cut(s) | 75, 433 |
| BtsIMutI | CAGTG | 1 cut(s) | 506 |
| BtuMI | TCGCGA | 1 cut(s) | 549 |
| BveI | ACCTGC | 1 cut(s) | 91 |
| Cac8I | GCNNGC | 1 cut(s) | 174 |
| CfoI | GCGC | 1 cut(s) | 115 |
| Cfr10I | RCCGGY | 2 cut(s) | 326, 360 |
| Cfr13I | GGNCC | 1 cut(s) | 73 |
| CseI | GACGC | 1 cut(s) | 300 |
| Csp6I | GTAC | 2 cut(s) | 186, 320 |
| CviAII | CATG | 2 cut(s) | 215, 223 |
| CviQI | GTAC | 2 cut(s) | 186, 320 |
| DdeI | CTNAG | 2 cut(s) | 278, 536 |
| DpnI | GATC | 3 cut(s) | 51, 339, 378 |
| DpnII | GATC | 3 cut(s) | 49, 337, 376 |
| EaeI | YGGCCR | 2 cut(s) | 348, 362 |
| EagI | CGGCCG | 1 cut(s) | 362 |
| EclXI | CGGCCG | 1 cut(s) | 362 |
| Eco130I | CCWWGG | 2 cut(s) | 132, 499 |
| Eco24I | GRGCYC | 2 cut(s) | 42, 195 |
| Eco31I | GGTCTC | 1 cut(s) | 104 |
| Eco52I | CGGCCG | 1 cut(s) | 362 |
| EcoT14I | CCWWGG | 2 cut(s) | 132, 499 |
| EcoT38I | GRGCYC | 2 cut(s) | 42, 195 |
| ErhI | CCWWGG | 2 cut(s) | 132, 499 |
| Esp3I | CGTCTC | 1 cut(s) | 283 |
| FaeI | CATG | 2 cut(s) | 218, 226 |
| FaiI | YATR | 7 cut(s) | 216, 224, 443, 451, 453, 554, 556 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 291, 323 |
| FaqI | GGGAC | 1 cut(s) | 428 |
| FatI | CATG | 2 cut(s) | 214, 222 |
| FauI | CCCGC | 1 cut(s) | 453 |
| FauNDI | CATATG | 1 cut(s) | 451 |
| Fnu4HI | GCNGC | 5 cut(s) | 212, 220, 348, 447, 458 |
| FokI | GGATG | 2 cut(s) | 82, 440 |
| FriOI | GRGCYC | 2 cut(s) | 42, 195 |
| Fsp4HI | GCNGC | 5 cut(s) | 212, 220, 348, 447, 458 |
| FspBI | CTAG | 2 cut(s) | 53, 173 |
| GlaI | GCGC | 1 cut(s) | 114 |
| GluI | GCNGC | 5 cut(s) | 212, 220, 348, 447, 458 |
| HaeII | RGCGCY | 1 cut(s) | 116 |
| HaeIII | GGCC | 5 cut(s) | 74, 131, 350, 364, 529 |
| HapII | CCGG | 3 cut(s) | 327, 361, 412 |
| HgaI | GACGC | 1 cut(s) | 300 |
| HhaI | GCGC | 1 cut(s) | 115 |
| Hin1II | CATG | 2 cut(s) | 218, 226 |
| Hin6I | GCGC | 1 cut(s) | 113 |
| HinP1I | GCGC | 1 cut(s) | 113 |
| HinfI | GANTC | 2 cut(s) | 90, 584 |
| HpaII | CCGG | 3 cut(s) | 327, 361, 412 |
| HphI | GGTGA | 1 cut(s) | 418 |
| Hpy166II | GTNNAC | 1 cut(s) | 332 |
| Hpy188I | TCNGA | 2 cut(s) | 69, 126 |
| Hpy188III | TCNNGA | 4 cut(s) | 399, 548, 579, 588 |
| Hpy8I | GTNNAC | 1 cut(s) | 332 |
| Hpy99I | CGWCG | 1 cut(s) | 594 |
| HpyAV | CCTTC | 4 cut(s) | 121, 151, 317, 540 |
| HpyCH4III | ACNGT | 3 cut(s) | 231, 487, 510 |
| HpyCH4IV | ACGT | 2 cut(s) | 318, 322 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 546, 573 |
| HpyF3I | CTNAG | 2 cut(s) | 278, 536 |
| HpySE526I | ACGT | 2 cut(s) | 318, 322 |
| Hsp92II | CATG | 2 cut(s) | 218, 226 |
| HspAI | GCGC | 1 cut(s) | 113 |
| Kzo9I | GATC | 3 cut(s) | 49, 337, 376 |
| LmnI | GCTCC | 2 cut(s) | 190, 503 |
| LpnPI | CCDG | 4 cut(s) | 96, 340, 374, 425 |
| LweI | GCATC | 2 cut(s) | 280, 554 |
| MaeI | CTAG | 2 cut(s) | 53, 173 |
| MaeII | ACGT | 2 cut(s) | 318, 322 |
| MalI | GATC | 3 cut(s) | 51, 339, 378 |
| MbiI | CCGCTC | 1 cut(s) | 457 |
| MboI | GATC | 3 cut(s) | 49, 337, 376 |
| MboII | GAAGA | 5 cut(s) | 99, 311, 476, 503, 620 |
| MflI | RGATCY | 1 cut(s) | 49 |
| MhlI | GDGCHC | 3 cut(s) | 42, 195, 508 |
| MnlI | CCTC | 5 cut(s) | 247, 397, 415, 418, 424 |
| MroXI | GAANNNNTTC | 1 cut(s) | 306 |
| MseI | TTAA | 2 cut(s) | 168, 512 |
| MspI | CCGG | 3 cut(s) | 327, 361, 412 |
| MspR9I | CCNGG | 1 cut(s) | 413 |
| Mva1269I | GAATGC | 1 cut(s) | 308 |
| MvnI | CGCG | 2 cut(s) | 393, 549 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 546, 573 |
| NciI | CCSGG | 1 cut(s) | 413 |
| NdeI | CATATG | 1 cut(s) | 451 |
| NdeII | GATC | 3 cut(s) | 49, 337, 376 |
| NheI | GCTAGC | 1 cut(s) | 172 |
| NlaIII | CATG | 2 cut(s) | 218, 226 |
| NlaIV | GGNNCC | 1 cut(s) | 192 |
| NruI | TCGCGA | 1 cut(s) | 549 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 586 |
| PctI | GAATGC | 1 cut(s) | 308 |
| PdmI | GAANNNNTTC | 1 cut(s) | 306 |
| PfeI | GAWTC | 2 cut(s) | 90, 584 |
| Pfl23II | CGTACG | 1 cut(s) | 319 |
| PkrI | GCNGC | 5 cut(s) | 213, 221, 349, 448, 459 |
| Ppu21I | YACGTR | 1 cut(s) | 323 |
| PspLI | CGTACG | 1 cut(s) | 319 |
| PspN4I | GGNNCC | 1 cut(s) | 192 |
| PspPI | GGNCC | 1 cut(s) | 73 |
| PsuI | RGATCY | 1 cut(s) | 49 |
| RruI | TCGCGA | 1 cut(s) | 549 |
| RsaI | GTAC | 2 cut(s) | 187, 321 |
| RsaNI | GTAC | 2 cut(s) | 186, 320 |
| SaqAI | TTAA | 2 cut(s) | 168, 512 |
| SatI | GCNGC | 5 cut(s) | 212, 220, 348, 447, 458 |
| Sau3AI | GATC | 3 cut(s) | 49, 337, 376 |
| Sau96I | GGNCC | 1 cut(s) | 73 |
| ScrFI | CCNGG | 1 cut(s) | 413 |
| SduI | GDGCHC | 3 cut(s) | 42, 195, 508 |
| SfaNI | GCATC | 2 cut(s) | 280, 554 |
| SmlI | CTYRAG | 1 cut(s) | 517 |
| SmoI | CTYRAG | 1 cut(s) | 517 |
| SspMI | CTAG | 2 cut(s) | 53, 173 |
| StyD4I | CCNGG | 1 cut(s) | 411 |
| StyI | CCWWGG | 2 cut(s) | 132, 499 |
| TaaI | ACNGT | 3 cut(s) | 231, 487, 510 |
| TaiI | ACGT | 2 cut(s) | 321, 325 |
| TaqI | TCGA | 3 cut(s) | 233, 523, 589 |
| TauI | GCSGC | 5 cut(s) | 214, 222, 350, 449, 460 |
| TfiI | GAWTC | 2 cut(s) | 90, 584 |
| Tru1I | TTAA | 2 cut(s) | 168, 512 |
| Tru9I | TTAA | 2 cut(s) | 168, 512 |
| TscAI | CASTG | 1 cut(s) | 513 |
| TspGWI | ACGGA | 3 cut(s) | 5, 203, 266 |
| TspRI | CASTG | 1 cut(s) | 513 |
| XmnI | GAANNNNTTC | 1 cut(s) | 306 |
| XspI | CTAG | 2 cut(s) | 53, 173 |