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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 404 |
| AcsI | RAATTY | 1 cut(s) | 348 |
| AfaI | GTAC | 1 cut(s) | 193 |
| AgsI | TTSAA | 1 cut(s) | 337 |
| AjiI | CACGTC | 1 cut(s) | 50 |
| AjnI | CCWGG | 2 cut(s) | 161, 264 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 383, 415 |
| AleI | CACNNNNGTG | 1 cut(s) | 107 |
| AluBI | AGCT | 4 cut(s) | 16, 117, 210, 370 |
| AluI | AGCT | 4 cut(s) | 16, 117, 210, 370 |
| AoxI | GGCC | 2 cut(s) | 201, 381 |
| ApeKI | GCWGC | 1 cut(s) | 64 |
| ApoI | RAATTY | 1 cut(s) | 348 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 126 |
| AspLEI | GCGC | 1 cut(s) | 435 |
| AspS9I | GGNCC | 2 cut(s) | 202, 262 |
| AsuC2I | CCSGG | 2 cut(s) | 180, 316 |
| AsuHPI | GGTGA | 1 cut(s) | 379 |
| AsuII | TTCGAA | 1 cut(s) | 120 |
| AvaII | GGWCC | 1 cut(s) | 262 |
| BbvI | GCAGC | 1 cut(s) | 76 |
| BccI | CCATC | 1 cut(s) | 249 |
| BciT130I | CCWGG | 2 cut(s) | 163, 266 |
| BcnI | CCSGG | 2 cut(s) | 180, 316 |
| BfaI | CTAG | 2 cut(s) | 17, 329 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 430 |
| BisI | GCNGC | 1 cut(s) | 65 |
| BlsI | GCNGC | 1 cut(s) | 66 |
| Bme1390I | CCNGG | 4 cut(s) | 163, 180, 266, 316 |
| Bme18I | GGWCC | 1 cut(s) | 262 |
| BmgBI | CACGTC | 1 cut(s) | 50 |
| BmgT120I | GGNCC | 2 cut(s) | 202, 262 |
| BmiI | GGNNCC | 2 cut(s) | 45, 360 |
| BmrFI | CCNGG | 4 cut(s) | 163, 180, 266, 316 |
| BmsI | GCATC | 3 cut(s) | 133, 167, 411 |
| Bpu10I | CCTNAGC | 1 cut(s) | 206 |
| Bpu14I | TTCGAA | 1 cut(s) | 120 |
| BpuMI | CCSGG | 2 cut(s) | 180, 316 |
| BsaAI | YACGTR | 1 cut(s) | 175 |
| BsaJI | CCNNGG | 2 cut(s) | 162, 354 |
| Bse118I | RCCGGY | 1 cut(s) | 217 |
| BseBI | CCWGG | 2 cut(s) | 163, 266 |
| BseDI | CCNNGG | 2 cut(s) | 162, 354 |
| BseGI | GGATG | 2 cut(s) | 158, 274 |
| BseMII | CTCAG | 3 cut(s) | 145, 197, 387 |
| BseRI | GAGGAG | 1 cut(s) | 299 |
| BseXI | GCAGC | 1 cut(s) | 76 |
| Bsh1236I | CGCG | 1 cut(s) | 404 |
| BshFI | GGCC | 2 cut(s) | 203, 383 |
| BsiSI | CCGG | 3 cut(s) | 179, 218, 315 |
| BslFI | GGGAC | 1 cut(s) | 181 |
| BsmFI | GGGAC | 1 cut(s) | 181 |
| BsmI | GAATGC | 1 cut(s) | 160 |
| BsnI | GGCC | 2 cut(s) | 203, 383 |
| Bsp119I | TTCGAA | 1 cut(s) | 120 |
| Bsp143I | GATC | 1 cut(s) | 440 |
| Bsp68I | TCGCGA | 1 cut(s) | 404 |
| BspANI | GGCC | 2 cut(s) | 203, 383 |
| BspCNI | CTCAG | 3 cut(s) | 144, 198, 388 |
| BspFNI | CGCG | 1 cut(s) | 404 |
| BspLI | GGNNCC | 2 cut(s) | 45, 360 |
| BspT104I | TTCGAA | 1 cut(s) | 120 |
| BsrFI | RCCGGY | 1 cut(s) | 217 |
| BssAI | RCCGGY | 1 cut(s) | 217 |
| BssECI | CCNNGG | 2 cut(s) | 162, 354 |
| BssMI | GATC | 1 cut(s) | 440 |
| BssT1I | CCWWGG | 1 cut(s) | 354 |
| Bst2UI | CCWGG | 2 cut(s) | 163, 266 |
| Bst4CI | ACNGT | 1 cut(s) | 196 |
| Bst6I | CTCTTC | 2 cut(s) | 249, 276 |
| BstBAI | YACGTR | 1 cut(s) | 175 |
| BstBI | TTCGAA | 1 cut(s) | 120 |
| BstC8I | GCNNGC | 1 cut(s) | 219 |
| BstDEI | CTNAG | 4 cut(s) | 6, 131, 206, 396 |
| BstF5I | GGATG | 2 cut(s) | 158, 274 |
| BstFNI | CGCG | 1 cut(s) | 404 |
| BstHHI | GCGC | 1 cut(s) | 435 |
| BstKTI | GATC | 1 cut(s) | 443 |
| BstMBI | GATC | 1 cut(s) | 440 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 184, 430 |
| BstNI | CCWGG | 2 cut(s) | 163, 266 |
| BstSCI | CCNGG | 4 cut(s) | 161, 178, 264, 314 |
| BstUI | CGCG | 1 cut(s) | 404 |
| BstV1I | GCAGC | 1 cut(s) | 76 |
| BsuRI | GGCC | 2 cut(s) | 203, 383 |
| BtrI | CACGTC | 1 cut(s) | 50 |
| BtsCI | GGATG | 2 cut(s) | 158, 274 |
| BtuMI | TCGCGA | 1 cut(s) | 404 |
| Cac8I | GCNNGC | 1 cut(s) | 219 |
| CfoI | GCGC | 1 cut(s) | 435 |
| Cfr10I | RCCGGY | 1 cut(s) | 217 |
| Cfr13I | GGNCC | 2 cut(s) | 202, 262 |
| Csp6I | GTAC | 1 cut(s) | 192 |
| CviAII | CATG | 2 cut(s) | 68, 436 |
| CviJI | RGCY | 9 cut(s) | 16, 117, 203, 210, 217, 221, 332, 370, 383 |
| CviKI_1 | RGCY | 9 cut(s) | 16, 117, 203, 210, 217, 221, 332, 370, 383 |
| CviQI | GTAC | 1 cut(s) | 192 |
| DdeI | CTNAG | 4 cut(s) | 6, 131, 206, 396 |
| DpnI | GATC | 1 cut(s) | 442 |
| DpnII | GATC | 1 cut(s) | 440 |
| Eam1104I | CTCTTC | 2 cut(s) | 249, 276 |
| EarI | CTCTTC | 2 cut(s) | 249, 276 |
| Eco130I | CCWWGG | 1 cut(s) | 354 |
| Eco32I | GATATC | 1 cut(s) | 295 |
| Eco47I | GGWCC | 1 cut(s) | 262 |
| EcoO109I | RGGNCCY | 1 cut(s) | 202 |
| EcoRII | CCWGG | 2 cut(s) | 161, 264 |
| EcoRV | GATATC | 1 cut(s) | 295 |
| EcoT14I | CCWWGG | 1 cut(s) | 354 |
| EcoT22I | ATGCAT | 1 cut(s) | 160 |
| ErhI | CCWWGG | 1 cut(s) | 354 |
| FaeI | CATG | 2 cut(s) | 71, 439 |
| FaqI | GGGAC | 1 cut(s) | 181 |
| FatI | CATG | 2 cut(s) | 67, 435 |
| Fnu4HI | GCNGC | 1 cut(s) | 65 |
| FokI | GGATG | 2 cut(s) | 145, 281 |
| Fsp4HI | GCNGC | 1 cut(s) | 65 |
| FspBI | CTAG | 2 cut(s) | 17, 329 |
| GlaI | GCGC | 1 cut(s) | 434 |
| GluI | GCNGC | 1 cut(s) | 65 |
| HaeIII | GGCC | 2 cut(s) | 203, 383 |
| HapII | CCGG | 3 cut(s) | 179, 218, 315 |
| HhaI | GCGC | 1 cut(s) | 435 |
| Hin1II | CATG | 2 cut(s) | 71, 439 |
| Hin6I | GCGC | 1 cut(s) | 433 |
| HinP1I | GCGC | 1 cut(s) | 433 |
| HincII | GTYRAC | 2 cut(s) | 53, 312 |
| HindII | GTYRAC | 2 cut(s) | 53, 312 |
| HinfI | GANTC | 2 cut(s) | 79, 325 |
| HpaII | CCGG | 3 cut(s) | 179, 218, 315 |
| HphI | GGTGA | 1 cut(s) | 379 |
| Hpy166II | GTNNAC | 2 cut(s) | 53, 312 |
| Hpy188I | TCNGA | 1 cut(s) | 134 |
| Hpy188III | TCNNGA | 1 cut(s) | 403 |
| Hpy8I | GTNNAC | 2 cut(s) | 53, 312 |
| Hpy99I | CGWCG | 2 cut(s) | 278, 448 |
| HpyAV | CCTTC | 1 cut(s) | 394 |
| HpyCH4III | ACNGT | 1 cut(s) | 196 |
| HpyCH4IV | ACGT | 2 cut(s) | 49, 174 |
| HpyCH4V | TGCA | 2 cut(s) | 146, 158 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 184, 430 |
| HpyF3I | CTNAG | 4 cut(s) | 6, 131, 206, 396 |
| HpySE526I | ACGT | 2 cut(s) | 49, 174 |
| Hsp92II | CATG | 2 cut(s) | 71, 439 |
| HspAI | GCGC | 1 cut(s) | 433 |
| KroI | GCCGGC | 1 cut(s) | 217 |
| KroNI | GCCGGC | 1 cut(s) | 219 |
| Kzo9I | GATC | 1 cut(s) | 440 |
| Lsp1109I | GCAGC | 1 cut(s) | 76 |
| LweI | GCATC | 3 cut(s) | 133, 167, 411 |
| MaeI | CTAG | 2 cut(s) | 17, 329 |
| MaeII | ACGT | 2 cut(s) | 49, 174 |
| MalI | GATC | 1 cut(s) | 442 |
| MboI | GATC | 1 cut(s) | 440 |
| MboII | GAAGA | 4 cut(s) | 119, 163, 266, 293 |
| MluCI | AATT | 1 cut(s) | 348 |
| MmeI | TCCRAC | 1 cut(s) | 6 |
| MnlI | CCTC | 6 cut(s) | 22, 100, 193, 250, 271, 277 |
| Mph1103I | ATGCAT | 1 cut(s) | 160 |
| MroNI | GCCGGC | 1 cut(s) | 217 |
| MroXI | GAANNNNTTC | 1 cut(s) | 126 |
| MseI | TTAA | 1 cut(s) | 366 |
| MslI | CAYNNNNRTG | 2 cut(s) | 107, 249 |
| MspI | CCGG | 3 cut(s) | 179, 218, 315 |
| MspR9I | CCNGG | 4 cut(s) | 163, 180, 266, 316 |
| Mva1269I | GAATGC | 1 cut(s) | 160 |
| MvaI | CCWGG | 2 cut(s) | 163, 266 |
| MvnI | CGCG | 1 cut(s) | 404 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 184, 430 |
| NaeI | GCCGGC | 1 cut(s) | 219 |
| NciI | CCSGG | 2 cut(s) | 180, 316 |
| NdeII | GATC | 1 cut(s) | 440 |
| NgoMIV | GCCGGC | 1 cut(s) | 217 |
| NlaIII | CATG | 2 cut(s) | 71, 439 |
| NlaIV | GGNNCC | 2 cut(s) | 45, 360 |
| NruI | TCGCGA | 1 cut(s) | 404 |
| NsiI | ATGCAT | 1 cut(s) | 160 |
| NspV | TTCGAA | 1 cut(s) | 120 |
| OliI | CACNNNNGTG | 1 cut(s) | 107 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 303 |
| PctI | GAATGC | 1 cut(s) | 160 |
| PdiI | GCCGGC | 1 cut(s) | 219 |
| PdmI | GAANNNNTTC | 1 cut(s) | 126 |
| PfeI | GAWTC | 2 cut(s) | 79, 325 |
| PfoI | TCCNGGA | 1 cut(s) | 264 |
| PkrI | GCNGC | 1 cut(s) | 66 |
| Ppu21I | YACGTR | 1 cut(s) | 175 |
| Psp6I | CCWGG | 2 cut(s) | 161, 264 |
| PspGI | CCWGG | 2 cut(s) | 161, 264 |
| PspN4I | GGNNCC | 2 cut(s) | 45, 360 |
| PspPI | GGNCC | 2 cut(s) | 202, 262 |
| RruI | TCGCGA | 1 cut(s) | 404 |
| RsaI | GTAC | 1 cut(s) | 193 |
| RsaNI | GTAC | 1 cut(s) | 192 |
| RseI | CAYNNNNRTG | 2 cut(s) | 107, 249 |
| SaqAI | TTAA | 1 cut(s) | 366 |
| SatI | GCNGC | 1 cut(s) | 65 |
| Sau3AI | GATC | 1 cut(s) | 440 |
| Sau96I | GGNCC | 2 cut(s) | 202, 262 |
| ScrFI | CCNGG | 4 cut(s) | 163, 180, 266, 316 |
| SfaNI | GCATC | 3 cut(s) | 133, 167, 411 |
| SfuI | TTCGAA | 1 cut(s) | 120 |
| SinI | GGWCC | 1 cut(s) | 262 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 107, 249 |
| Sse9I | AATT | 1 cut(s) | 348 |
| SspMI | CTAG | 2 cut(s) | 17, 329 |
| StyD4I | CCNGG | 4 cut(s) | 161, 178, 264, 314 |
| StyI | CCWWGG | 1 cut(s) | 354 |
| TaaI | ACNGT | 1 cut(s) | 196 |
| TaiI | ACGT | 2 cut(s) | 52, 177 |
| TaqI | TCGA | 3 cut(s) | 86, 120, 443 |
| TasI | AATT | 1 cut(s) | 348 |
| TfiI | GAWTC | 2 cut(s) | 79, 325 |
| Tru1I | TTAA | 1 cut(s) | 366 |
| Tru9I | TTAA | 1 cut(s) | 366 |
| TseI | GCWGC | 1 cut(s) | 64 |
| TspDTI | ATGAA | 1 cut(s) | 267 |
| VpaK11BI | GGWCC | 1 cut(s) | 262 |
| XapI | RAATTY | 1 cut(s) | 348 |
| XmnI | GAANNNNTTC | 1 cut(s) | 126 |
| XspI | CTAG | 2 cut(s) | 17, 329 |
| Zsp2I | ATGCAT | 1 cut(s) | 160 |