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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 878 |
| AccB1I | GGYRCC | 1 cut(s) | 937 |
| AccI | GTMKAC | 1 cut(s) | 247 |
| AccII | CGCG | 3 cut(s) | 110, 395, 662 |
| AciI | CCGC | 7 cut(s) | 60, 108, 236, 579, 660, 906, 944 |
| AclWI | GGATC | 4 cut(s) | 408, 421, 488, 767 |
| AcoI | YGGCCR | 1 cut(s) | 435 |
| AcsI | RAATTY | 1 cut(s) | 196 |
| AcuI | CTGAAG | 2 cut(s) | 60, 369 |
| AcyI | GRCGYC | 2 cut(s) | 875, 938 |
| AfaI | GTAC | 1 cut(s) | 586 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 238, 460, 536, 857 |
| AgsI | TTSAA | 6 cut(s) | 191, 265, 476, 694, 758, 812 |
| AjnI | CCWGG | 2 cut(s) | 429, 683 |
| AluBI | AGCT | 2 cut(s) | 289, 358 |
| AluI | AGCT | 2 cut(s) | 289, 358 |
| Alw21I | GWGCWC | 1 cut(s) | 768 |
| Alw26I | GTCTC | 1 cut(s) | 620 |
| Alw44I | GTGCAC | 1 cut(s) | 764 |
| AlwI | GGATC | 4 cut(s) | 408, 421, 488, 767 |
| Ama87I | CYCGRG | 1 cut(s) | 561 |
| AoxI | GGCC | 5 cut(s) | 435, 447, 462, 657, 708 |
| ApaLI | GTGCAC | 1 cut(s) | 764 |
| ApeKI | GCWGC | 2 cut(s) | 203, 833 |
| ApoI | RAATTY | 1 cut(s) | 196 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 295, 327, 673, 705 |
| AspLEI | GCGC | 2 cut(s) | 116, 940 |
| AspS9I | GGNCC | 3 cut(s) | 241, 681, 708 |
| AsuC2I | CCSGG | 1 cut(s) | 806 |
| AvaI | CYCGRG | 1 cut(s) | 561 |
| AvaII | GGWCC | 2 cut(s) | 241, 681 |
| BaeGI | GKGCMC | 2 cut(s) | 298, 768 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 591, 624 |
| BalI | TGGCCA | 1 cut(s) | 437 |
| BamHI | GGATCC | 1 cut(s) | 413 |
| BanI | GGYRCC | 1 cut(s) | 937 |
| Bbv12I | GWGCWC | 1 cut(s) | 768 |
| BbvI | GCAGC | 2 cut(s) | 190, 845 |
| BccI | CCATC | 3 cut(s) | 214, 314, 893 |
| BceAI | ACGGC | 1 cut(s) | 266 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 361, 395 |
| BciT130I | CCWGG | 2 cut(s) | 431, 685 |
| BclI | TGATCA | 1 cut(s) | 457 |
| BcnI | CCSGG | 1 cut(s) | 806 |
| BcoDI | GTCTC | 1 cut(s) | 620 |
| BfaI | CTAG | 4 cut(s) | 90, 290, 498, 509 |
| BfmI | CTRYAG | 1 cut(s) | 831 |
| BfoI | RGCGCY | 1 cut(s) | 941 |
| BglII | AGATCT | 1 cut(s) | 267 |
| BisI | GCNGC | 6 cut(s) | 108, 204, 580, 660, 834, 907 |
| BlsI | GCNGC | 6 cut(s) | 109, 205, 581, 661, 835, 908 |
| Bme1390I | CCNGG | 3 cut(s) | 431, 685, 806 |
| Bme18I | GGWCC | 2 cut(s) | 241, 681 |
| BmeT110I | CYCGRG | 1 cut(s) | 561 |
| BmgT120I | GGNCC | 3 cut(s) | 241, 681, 708 |
| BmiI | GGNNCC | 4 cut(s) | 415, 682, 709, 939 |
| BmrFI | CCNGG | 3 cut(s) | 431, 685, 806 |
| BmrI | ACTGGG | 1 cut(s) | 497 |
| BmsI | GCATC | 1 cut(s) | 102 |
| BmuI | ACTGGG | 1 cut(s) | 497 |
| BpuEI | CTTGAG | 2 cut(s) | 305, 501 |
| BpuMI | CCSGG | 1 cut(s) | 806 |
| BsaAI | YACGTR | 1 cut(s) | 763 |
| BsaHI | GRCGYC | 2 cut(s) | 875, 938 |
| BsaJI | CCNNGG | 1 cut(s) | 429 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 238, 460, 536, 857 |
| Bse1I | ACTGG | 2 cut(s) | 492, 851 |
| Bse3DI | GCAATG | 1 cut(s) | 645 |
| BseBI | CCWGG | 2 cut(s) | 431, 685 |
| BseDI | CCNNGG | 1 cut(s) | 429 |
| BseGI | GGATG | 3 cut(s) | 28, 325, 593 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 238, 460, 536, 857 |
| BseMI | GCAATG | 1 cut(s) | 645 |
| BseMII | CTCAG | 1 cut(s) | 113 |
| BseNI | ACTGG | 2 cut(s) | 492, 851 |
| BseRI | GAGGAG | 1 cut(s) | 30 |
| BseSI | GKGCMC | 2 cut(s) | 298, 768 |
| BseXI | GCAGC | 2 cut(s) | 190, 845 |
| BseYI | CCCAGC | 1 cut(s) | 63 |
| Bsh1236I | CGCG | 3 cut(s) | 110, 395, 662 |
| Bsh1285I | CGRYCG | 1 cut(s) | 805 |
| BshFI | GGCC | 5 cut(s) | 437, 449, 464, 659, 710 |
| BshNI | GGYRCC | 1 cut(s) | 937 |
| BsiEI | CGRYCG | 1 cut(s) | 805 |
| BsiHKAI | GWGCWC | 1 cut(s) | 768 |
| BsiHKCI | CYCGRG | 1 cut(s) | 561 |
| BsiSI | CCGG | 1 cut(s) | 805 |
| BslFI | GGGAC | 2 cut(s) | 511, 694 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 238, 460, 536, 857 |
| BsmAI | GTCTC | 1 cut(s) | 620 |
| BsmFI | GGGAC | 2 cut(s) | 511, 694 |
| BsnI | GGCC | 5 cut(s) | 437, 449, 464, 659, 710 |
| BsoBI | CYCGRG | 1 cut(s) | 561 |
| Bsp1286I | GDGCHC | 2 cut(s) | 298, 768 |
| Bsp143I | GATC | 6 cut(s) | 267, 413, 457, 493, 772, 948 |
| Bsp68I | TCGCGA | 1 cut(s) | 395 |
| BspACI | CCGC | 7 cut(s) | 60, 108, 236, 579, 660, 906, 944 |
| BspANI | GGCC | 5 cut(s) | 437, 449, 464, 659, 710 |
| BspCNI | CTCAG | 1 cut(s) | 112 |
| BspFNI | CGCG | 3 cut(s) | 110, 395, 662 |
| BspLI | GGNNCC | 4 cut(s) | 415, 682, 709, 939 |
| BspMAI | CTGCAG | 1 cut(s) | 835 |
| BspPI | GGATC | 4 cut(s) | 408, 421, 488, 767 |
| BspQI | GCTCTTC | 1 cut(s) | 165 |
| BspT107I | GGYRCC | 1 cut(s) | 937 |
| BsrDI | GCAATG | 1 cut(s) | 645 |
| BsrI | ACTGG | 2 cut(s) | 492, 851 |
| BssECI | CCNNGG | 1 cut(s) | 429 |
| BssMI | GATC | 6 cut(s) | 267, 413, 457, 493, 772, 948 |
| BssNI | GRCGYC | 2 cut(s) | 875, 938 |
| Bst2UI | CCWGG | 2 cut(s) | 431, 685 |
| Bst4CI | ACNGT | 1 cut(s) | 245 |
| Bst6I | CTCTTC | 1 cut(s) | 165 |
| BstACI | GRCGYC | 2 cut(s) | 875, 938 |
| BstBAI | YACGTR | 1 cut(s) | 763 |
| BstC8I | GCNNGC | 3 cut(s) | 79, 112, 370 |
| BstDEI | CTNAG | 1 cut(s) | 99 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 236, 458 |
| BstF5I | GGATG | 3 cut(s) | 28, 325, 593 |
| BstFNI | CGCG | 3 cut(s) | 110, 395, 662 |
| BstH2I | RGCGCY | 1 cut(s) | 941 |
| BstHHI | GCGC | 2 cut(s) | 116, 940 |
| BstKTI | GATC | 6 cut(s) | 270, 416, 460, 496, 775, 951 |
| BstMAI | GTCTC | 1 cut(s) | 620 |
| BstMBI | GATC | 6 cut(s) | 267, 413, 457, 493, 772, 948 |
| BstMCI | CGRYCG | 1 cut(s) | 805 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 113, 935 |
| BstNI | CCWGG | 2 cut(s) | 431, 685 |
| BstSCI | CCNGG | 3 cut(s) | 429, 683, 804 |
| BstSFI | CTRYAG | 1 cut(s) | 831 |
| BstSLI | GKGCMC | 2 cut(s) | 298, 768 |
| BstUI | CGCG | 3 cut(s) | 110, 395, 662 |
| BstV1I | GCAGC | 2 cut(s) | 190, 845 |
| BstX2I | RGATCY | 3 cut(s) | 267, 413, 493 |
| BstYI | RGATCY | 3 cut(s) | 267, 413, 493 |
| BsuRI | GGCC | 5 cut(s) | 437, 449, 464, 659, 710 |
| BtgZI | GCGATG | 1 cut(s) | 364 |
| BtsCI | GGATG | 3 cut(s) | 28, 325, 593 |
| BtsI | GCAGTG | 1 cut(s) | 828 |
| BtsIMutI | CAGTG | 2 cut(s) | 485, 828 |
| BtuMI | TCGCGA | 1 cut(s) | 395 |
| Cac8I | GCNNGC | 3 cut(s) | 79, 112, 370 |
| CfoI | GCGC | 2 cut(s) | 116, 940 |
| Cfr13I | GGNCC | 3 cut(s) | 241, 681, 708 |
| CseI | GACGC | 1 cut(s) | 651 |
| Csp6I | GTAC | 1 cut(s) | 585 |
| CviAII | CATG | 3 cut(s) | 212, 325, 439 |
| CviQI | GTAC | 1 cut(s) | 585 |
| DdeI | CTNAG | 1 cut(s) | 99 |
| DinI | GGCGCC | 1 cut(s) | 939 |
| DpnI | GATC | 6 cut(s) | 269, 415, 459, 495, 774, 950 |
| DpnII | GATC | 6 cut(s) | 267, 413, 457, 493, 772, 948 |
| EaeI | YGGCCR | 1 cut(s) | 435 |
| Eam1104I | CTCTTC | 1 cut(s) | 165 |
| EarI | CTCTTC | 1 cut(s) | 165 |
| Eco47I | GGWCC | 2 cut(s) | 241, 681 |
| Eco57I | CTGAAG | 2 cut(s) | 60, 369 |
| Eco88I | CYCGRG | 1 cut(s) | 561 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 236, 458 |
| EcoO109I | RGGNCCY | 1 cut(s) | 708 |
| EcoRI | GAATTC | 1 cut(s) | 196 |
| EcoRII | CCWGG | 2 cut(s) | 429, 683 |
| EgeI | GGCGCC | 1 cut(s) | 939 |
| EheI | GGCGCC | 1 cut(s) | 939 |
| FaeI | CATG | 3 cut(s) | 215, 328, 442 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 162, 194 |
| FaqI | GGGAC | 2 cut(s) | 511, 694 |
| FatI | CATG | 3 cut(s) | 211, 324, 438 |
| FbaI | TGATCA | 1 cut(s) | 457 |
| FblI | GTMKAC | 1 cut(s) | 247 |
| Fnu4HI | GCNGC | 6 cut(s) | 108, 204, 580, 660, 834, 907 |
| FokI | GGATG | 3 cut(s) | 15, 332, 580 |
| Fsp4HI | GCNGC | 6 cut(s) | 108, 204, 580, 660, 834, 907 |
| FspBI | CTAG | 4 cut(s) | 90, 290, 498, 509 |
| GlaI | GCGC | 2 cut(s) | 115, 939 |
| GluI | GCNGC | 6 cut(s) | 108, 204, 580, 660, 834, 907 |
| GsaI | CCCAGC | 1 cut(s) | 67 |
| HaeII | RGCGCY | 1 cut(s) | 941 |
| HaeIII | GGCC | 5 cut(s) | 437, 449, 464, 659, 710 |
| HapII | CCGG | 1 cut(s) | 805 |
| HgaI | GACGC | 1 cut(s) | 651 |
| HhaI | GCGC | 2 cut(s) | 116, 940 |
| Hin1I | GRCGYC | 2 cut(s) | 875, 938 |
| Hin1II | CATG | 3 cut(s) | 215, 328, 442 |
| Hin6I | GCGC | 2 cut(s) | 114, 938 |
| HinP1I | GCGC | 2 cut(s) | 114, 938 |
| HincII | GTYRAC | 3 cut(s) | 6, 248, 879 |
| HindII | GTYRAC | 3 cut(s) | 6, 248, 879 |
| HpaII | CCGG | 1 cut(s) | 805 |
| Hpy166II | GTNNAC | 4 cut(s) | 6, 248, 766, 879 |
| Hpy188I | TCNGA | 3 cut(s) | 388, 617, 953 |
| Hpy188III | TCNNGA | 6 cut(s) | 152, 265, 277, 394, 424, 563 |
| Hpy8I | GTNNAC | 4 cut(s) | 6, 248, 766, 879 |
| Hpy99I | CGWCG | 5 cut(s) | 249, 252, 356, 752, 871 |
| HpyAV | CCTTC | 6 cut(s) | 35, 197, 404, 439, 698, 817 |
| HpyCH4III | ACNGT | 1 cut(s) | 245 |
| HpyCH4IV | ACGT | 4 cut(s) | 86, 762, 866, 875 |
| HpyCH4V | TGCA | 5 cut(s) | 206, 340, 442, 766, 833 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 113, 935 |
| HpyF3I | CTNAG | 1 cut(s) | 99 |
| HpySE526I | ACGT | 4 cut(s) | 86, 762, 866, 875 |
| Hsp92I | GRCGYC | 2 cut(s) | 875, 938 |
| Hsp92II | CATG | 3 cut(s) | 215, 328, 442 |
| HspAI | GCGC | 2 cut(s) | 114, 938 |
| KasI | GGCGCC | 1 cut(s) | 937 |
| Ksp22I | TGATCA | 1 cut(s) | 457 |
| Kzo9I | GATC | 6 cut(s) | 267, 413, 457, 493, 772, 948 |
| LguI | GCTCTTC | 1 cut(s) | 165 |
| Lsp1109I | GCAGC | 2 cut(s) | 190, 845 |
| LweI | GCATC | 1 cut(s) | 102 |
| MaeI | CTAG | 4 cut(s) | 90, 290, 498, 509 |
| MaeII | ACGT | 4 cut(s) | 86, 762, 866, 875 |
| MaeIII | GTNAC | 4 cut(s) | 424, 598, 670, 826 |
| MalI | GATC | 6 cut(s) | 269, 415, 459, 495, 774, 950 |
| MboI | GATC | 6 cut(s) | 267, 413, 457, 493, 772, 948 |
| MboII | GAAGA | 4 cut(s) | 182, 229, 355, 661 |
| MfeI | CAATTG | 1 cut(s) | 314 |
| MflI | RGATCY | 3 cut(s) | 267, 413, 493 |
| MhlI | GDGCHC | 2 cut(s) | 298, 768 |
| MlsI | TGGCCA | 1 cut(s) | 437 |
| MluCI | AATT | 5 cut(s) | 117, 196, 314, 399, 541 |
| MluNI | TGGCCA | 1 cut(s) | 437 |
| Mly113I | GGCGCC | 1 cut(s) | 938 |
| MmeI | TCCRAC | 1 cut(s) | 441 |
| MnlI | CCTC | 8 cut(s) | 8, 11, 232, 273, 463, 611, 721, 896 |
| Mox20I | TGGCCA | 1 cut(s) | 437 |
| MscI | TGGCCA | 1 cut(s) | 437 |
| MseI | TTAA | 2 cut(s) | 180, 921 |
| Msp20I | TGGCCA | 1 cut(s) | 437 |
| MspA1I | CMGCKG | 1 cut(s) | 946 |
| MspI | CCGG | 1 cut(s) | 805 |
| MspR9I | CCNGG | 3 cut(s) | 431, 685, 806 |
| MunI | CAATTG | 1 cut(s) | 314 |
| MvaI | CCWGG | 2 cut(s) | 431, 685 |
| MvnI | CGCG | 3 cut(s) | 110, 395, 662 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 113, 935 |
| NarI | GGCGCC | 1 cut(s) | 938 |
| NciI | CCSGG | 1 cut(s) | 806 |
| NdeII | GATC | 6 cut(s) | 267, 413, 457, 493, 772, 948 |
| NlaIII | CATG | 3 cut(s) | 215, 328, 442 |
| NlaIV | GGNNCC | 4 cut(s) | 415, 682, 709, 939 |
| NmuCI | GTSAC | 3 cut(s) | 424, 670, 826 |
| NruI | TCGCGA | 1 cut(s) | 395 |
| PaeR7I | CTCGAG | 1 cut(s) | 561 |
| PciSI | GCTCTTC | 1 cut(s) | 165 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 768, 872 |
| PkrI | GCNGC | 6 cut(s) | 109, 205, 581, 661, 835, 908 |
| PluTI | GGCGCC | 1 cut(s) | 941 |
| Ppu21I | YACGTR | 1 cut(s) | 763 |
| Psp6I | CCWGG | 2 cut(s) | 429, 683 |
| PspFI | CCCAGC | 1 cut(s) | 63 |
| PspGI | CCWGG | 2 cut(s) | 429, 683 |
| PspN4I | GGNNCC | 4 cut(s) | 415, 682, 709, 939 |
| PspPI | GGNCC | 3 cut(s) | 241, 681, 708 |
| PstI | CTGCAG | 1 cut(s) | 835 |
| PsuI | RGATCY | 3 cut(s) | 267, 413, 493 |
| RruI | TCGCGA | 1 cut(s) | 395 |
| RsaI | GTAC | 1 cut(s) | 586 |
| RsaNI | GTAC | 1 cut(s) | 585 |
| SalI | GTCGAC | 1 cut(s) | 246 |
| SapI | GCTCTTC | 1 cut(s) | 165 |
| SaqAI | TTAA | 2 cut(s) | 180, 921 |
| SatI | GCNGC | 6 cut(s) | 108, 204, 580, 660, 834, 907 |
| Sau3AI | GATC | 6 cut(s) | 267, 413, 457, 493, 772, 948 |
| Sau96I | GGNCC | 3 cut(s) | 241, 681, 708 |
| ScrFI | CCNGG | 3 cut(s) | 431, 685, 806 |
| SduI | GDGCHC | 2 cut(s) | 298, 768 |
| SfaNI | GCATC | 1 cut(s) | 102 |
| SfcI | CTRYAG | 1 cut(s) | 831 |
| SfoI | GGCGCC | 1 cut(s) | 939 |
| Sfr274I | CTCGAG | 1 cut(s) | 561 |
| SgrDI | CGTCGACG | 1 cut(s) | 246 |
| SinI | GGWCC | 2 cut(s) | 241, 681 |
| SlaI | CTCGAG | 1 cut(s) | 561 |
| SmlI | CTYRAG | 3 cut(s) | 284, 516, 561 |
| SmoI | CTYRAG | 3 cut(s) | 284, 516, 561 |
| Sse9I | AATT | 5 cut(s) | 117, 196, 314, 399, 541 |
| SsiI | CCGC | 7 cut(s) | 60, 108, 236, 579, 660, 906, 944 |
| SspDI | GGCGCC | 1 cut(s) | 937 |
| SspMI | CTAG | 4 cut(s) | 90, 290, 498, 509 |
| StyD4I | CCNGG | 3 cut(s) | 429, 683, 804 |
| TaaI | ACNGT | 1 cut(s) | 245 |
| TaiI | ACGT | 4 cut(s) | 89, 765, 869, 878 |
| TaqI | TCGA | 3 cut(s) | 247, 562, 801 |
| TaqII | GACCGA | 2 cut(s) | 262, 911 |
| TasI | AATT | 5 cut(s) | 117, 196, 314, 399, 541 |
| TatI | WGTACW | 1 cut(s) | 584 |
| TauI | GCSGC | 4 cut(s) | 110, 582, 662, 909 |
| Tru1I | TTAA | 2 cut(s) | 180, 921 |
| Tru9I | TTAA | 2 cut(s) | 180, 921 |
| TscAI | CASTG | 2 cut(s) | 492, 835 |
| TseFI | GTSAC | 3 cut(s) | 424, 670, 826 |
| TseI | GCWGC | 2 cut(s) | 203, 833 |
| Tsp45I | GTSAC | 3 cut(s) | 424, 670, 826 |
| TspDTI | ATGAA | 2 cut(s) | 341, 717 |
| TspGWI | ACGGA | 2 cut(s) | 586, 765 |
| TspRI | CASTG | 2 cut(s) | 492, 835 |
| VneI | GTGCAC | 1 cut(s) | 764 |
| VpaK11BI | GGWCC | 2 cut(s) | 241, 681 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 236, 458 |
| XapI | RAATTY | 1 cut(s) | 196 |
| XhoI | CTCGAG | 1 cut(s) | 561 |
| XmiI | GTMKAC | 1 cut(s) | 247 |
| XspI | CTAG | 4 cut(s) | 90, 290, 498, 509 |
| ZraI | GACGTC | 1 cut(s) | 876 |