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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 92 |
| AccII | CGCG | 2 cut(s) | 35, 254 |
| AciI | CCGC | 4 cut(s) | 45, 222, 241, 252 |
| AclWI | GGATC | 2 cut(s) | 137, 150 |
| AcoI | YGGCCR | 2 cut(s) | 84, 169 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 349 |
| AgsI | TTSAA | 1 cut(s) | 127 |
| AjnI | CCWGG | 1 cut(s) | 108 |
| AlwI | GGATC | 2 cut(s) | 137, 150 |
| AoxI | GGCC | 4 cut(s) | 84, 169, 215, 351 |
| ApaI | GGGCCC | 1 cut(s) | 355 |
| AspA2I | CCTAGG | 1 cut(s) | 212 |
| AspLEI | GCGC | 4 cut(s) | 30, 65, 93, 296 |
| AspS9I | GGNCC | 2 cut(s) | 351, 352 |
| AsuHPI | GGTGA | 1 cut(s) | 236 |
| AsuII | TTCGAA | 1 cut(s) | 233 |
| AvrII | CCTAGG | 1 cut(s) | 212 |
| BaeGI | GKGCMC | 1 cut(s) | 355 |
| BalI | TGGCCA | 2 cut(s) | 86, 171 |
| BamHI | GGATCC | 1 cut(s) | 142 |
| BanII | GRGCYC | 1 cut(s) | 355 |
| BbsI | GAAGAC | 1 cut(s) | 41 |
| BccI | CCATC | 1 cut(s) | 53 |
| BceAI | ACGGC | 1 cut(s) | 160 |
| BciT130I | CCWGG | 1 cut(s) | 110 |
| BfaI | CTAG | 1 cut(s) | 213 |
| BfoI | RGCGCY | 1 cut(s) | 66 |
| BlnI | CCTAGG | 1 cut(s) | 212 |
| Bme1390I | CCNGG | 1 cut(s) | 110 |
| BmgT120I | GGNCC | 2 cut(s) | 351, 352 |
| BmiI | GGNNCC | 3 cut(s) | 144, 163, 353 |
| BmrFI | CCNGG | 1 cut(s) | 110 |
| BmsI | GCATC | 2 cut(s) | 39, 198 |
| BpiI | GAAGAC | 1 cut(s) | 41 |
| Bpu14I | TTCGAA | 1 cut(s) | 233 |
| BsaAI | YACGTR | 1 cut(s) | 362 |
| BsaJI | CCNNGG | 5 cut(s) | 78, 157, 166, 195, 212 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 349 |
| BseBI | CCWGG | 1 cut(s) | 110 |
| BseDI | CCNNGG | 5 cut(s) | 78, 157, 166, 195, 212 |
| BseGI | GGATG | 2 cut(s) | 10, 64 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 349 |
| BseRI | GAGGAG | 4 cut(s) | 323, 326, 329, 332 |
| BseSI | GKGCMC | 1 cut(s) | 355 |
| Bsh1236I | CGCG | 2 cut(s) | 35, 254 |
| BshFI | GGCC | 4 cut(s) | 86, 171, 217, 353 |
| BsiSI | CCGG | 1 cut(s) | 180 |
| BslFI | GGGAC | 1 cut(s) | 287 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 349 |
| BsmFI | GGGAC | 1 cut(s) | 287 |
| BsnI | GGCC | 4 cut(s) | 86, 171, 217, 353 |
| Bsp119I | TTCGAA | 1 cut(s) | 233 |
| Bsp120I | GGGCCC | 1 cut(s) | 351 |
| Bsp1286I | GDGCHC | 1 cut(s) | 355 |
| Bsp143I | GATC | 1 cut(s) | 142 |
| BspACI | CCGC | 4 cut(s) | 45, 222, 241, 252 |
| BspANI | GGCC | 4 cut(s) | 86, 171, 217, 353 |
| BspFNI | CGCG | 2 cut(s) | 35, 254 |
| BspLI | GGNNCC | 3 cut(s) | 144, 163, 353 |
| BspPI | GGATC | 2 cut(s) | 137, 150 |
| BspT104I | TTCGAA | 1 cut(s) | 233 |
| BssECI | CCNNGG | 5 cut(s) | 78, 157, 166, 195, 212 |
| BssMI | GATC | 1 cut(s) | 142 |
| BssT1I | CCWWGG | 4 cut(s) | 157, 166, 195, 212 |
| Bst2UI | CCWGG | 1 cut(s) | 110 |
| Bst6I | CTCTTC | 1 cut(s) | 235 |
| BstBAI | YACGTR | 1 cut(s) | 362 |
| BstBI | TTCGAA | 1 cut(s) | 233 |
| BstC8I | GCNNGC | 2 cut(s) | 173, 312 |
| BstF5I | GGATG | 2 cut(s) | 10, 64 |
| BstFNI | CGCG | 2 cut(s) | 35, 254 |
| BstH2I | RGCGCY | 1 cut(s) | 66 |
| BstHHI | GCGC | 4 cut(s) | 30, 65, 93, 296 |
| BstKTI | GATC | 1 cut(s) | 145 |
| BstMBI | GATC | 1 cut(s) | 142 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 51, 60, 71, 168 |
| BstNI | CCWGG | 1 cut(s) | 110 |
| BstSCI | CCNGG | 1 cut(s) | 108 |
| BstSLI | GKGCMC | 1 cut(s) | 355 |
| BstSNI | TACGTA | 1 cut(s) | 362 |
| BstUI | CGCG | 2 cut(s) | 35, 254 |
| BstV2I | GAAGAC | 1 cut(s) | 41 |
| BstX2I | RGATCY | 1 cut(s) | 142 |
| BstYI | RGATCY | 1 cut(s) | 142 |
| BsuRI | GGCC | 4 cut(s) | 86, 171, 217, 353 |
| BtgZI | GCGATG | 1 cut(s) | 16 |
| BtsCI | GGATG | 2 cut(s) | 10, 64 |
| Cac8I | GCNNGC | 2 cut(s) | 173, 312 |
| CfoI | GCGC | 4 cut(s) | 30, 65, 93, 296 |
| Cfr13I | GGNCC | 2 cut(s) | 351, 352 |
| CseI | GACGC | 1 cut(s) | 24 |
| CviAII | CATG | 1 cut(s) | 88 |
| CviJI | RGCY | 9 cut(s) | 86, 98, 162, 171, 175, 183, 194, 217, 353 |
| CviKI_1 | RGCY | 9 cut(s) | 86, 98, 162, 171, 175, 183, 194, 217, 353 |
| DpnI | GATC | 1 cut(s) | 144 |
| DpnII | GATC | 1 cut(s) | 142 |
| EaeI | YGGCCR | 2 cut(s) | 84, 169 |
| Eam1104I | CTCTTC | 1 cut(s) | 235 |
| EarI | CTCTTC | 1 cut(s) | 235 |
| Eco105I | TACGTA | 1 cut(s) | 362 |
| Eco130I | CCWWGG | 4 cut(s) | 157, 166, 195, 212 |
| Eco24I | GRGCYC | 1 cut(s) | 355 |
| EcoRII | CCWGG | 1 cut(s) | 108 |
| EcoT14I | CCWWGG | 4 cut(s) | 157, 166, 195, 212 |
| EcoT38I | GRGCYC | 1 cut(s) | 355 |
| ErhI | CCWWGG | 4 cut(s) | 157, 166, 195, 212 |
| FaeI | CATG | 1 cut(s) | 91 |
| FaiI | YATR | 5 cut(s) | 42, 89, 263, 317, 322 |
| FaqI | GGGAC | 1 cut(s) | 287 |
| FatI | CATG | 1 cut(s) | 87 |
| FauI | CCCGC | 1 cut(s) | 259 |
| FokI | GGATG | 2 cut(s) | 17, 71 |
| FriOI | GRGCYC | 1 cut(s) | 355 |
| FspBI | CTAG | 1 cut(s) | 213 |
| FspI | TGCGCA | 1 cut(s) | 92 |
| GlaI | GCGC | 4 cut(s) | 29, 64, 92, 295 |
| HaeII | RGCGCY | 1 cut(s) | 66 |
| HaeIII | GGCC | 4 cut(s) | 86, 171, 217, 353 |
| HapII | CCGG | 1 cut(s) | 180 |
| HgaI | GACGC | 1 cut(s) | 24 |
| HhaI | GCGC | 4 cut(s) | 30, 65, 93, 296 |
| Hin1II | CATG | 1 cut(s) | 91 |
| Hin6I | GCGC | 4 cut(s) | 28, 63, 91, 294 |
| HinP1I | GCGC | 4 cut(s) | 28, 63, 91, 294 |
| HinfI | GANTC | 1 cut(s) | 227 |
| HpaII | CCGG | 1 cut(s) | 180 |
| HphI | GGTGA | 1 cut(s) | 236 |
| Hpy166II | GTNNAC | 1 cut(s) | 287 |
| Hpy8I | GTNNAC | 1 cut(s) | 287 |
| Hpy99I | CGWCG | 1 cut(s) | 40 |
| HpyAV | CCTTC | 2 cut(s) | 264, 356 |
| HpyCH4IV | ACGT | 1 cut(s) | 361 |
| HpyCH4V | TGCA | 2 cut(s) | 261, 304 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 51, 60, 71, 168 |
| HpySE526I | ACGT | 1 cut(s) | 361 |
| Hsp92II | CATG | 1 cut(s) | 91 |
| HspAI | GCGC | 4 cut(s) | 28, 63, 91, 294 |
| Kzo9I | GATC | 1 cut(s) | 142 |
| LmnI | GCTCC | 1 cut(s) | 167 |
| LpnPI | CCDG | 4 cut(s) | 95, 122, 185, 193 |
| LweI | GCATC | 2 cut(s) | 39, 198 |
| MaeI | CTAG | 1 cut(s) | 213 |
| MaeII | ACGT | 1 cut(s) | 361 |
| MaeIII | GTNAC | 1 cut(s) | 224 |
| MalI | GATC | 1 cut(s) | 144 |
| MboI | GATC | 1 cut(s) | 142 |
| MboII | GAAGA | 3 cut(s) | 41, 222, 247 |
| MflI | RGATCY | 1 cut(s) | 142 |
| MhlI | GDGCHC | 1 cut(s) | 355 |
| MlsI | TGGCCA | 2 cut(s) | 86, 171 |
| MluNI | TGGCCA | 2 cut(s) | 86, 171 |
| MlyI | GAGTC | 1 cut(s) | 221 |
| MnlI | CCTC | 7 cut(s) | 73, 80, 341, 344, 347, 350, 353 |
| Mox20I | TGGCCA | 2 cut(s) | 86, 171 |
| MscI | TGGCCA | 2 cut(s) | 86, 171 |
| MseI | TTAA | 1 cut(s) | 15 |
| Msp20I | TGGCCA | 2 cut(s) | 86, 171 |
| MspI | CCGG | 1 cut(s) | 180 |
| MspR9I | CCNGG | 1 cut(s) | 110 |
| MvaI | CCWGG | 1 cut(s) | 110 |
| MvnI | CGCG | 2 cut(s) | 35, 254 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 51, 60, 71, 168 |
| NdeII | GATC | 1 cut(s) | 142 |
| NlaIII | CATG | 1 cut(s) | 91 |
| NlaIV | GGNNCC | 3 cut(s) | 144, 163, 353 |
| NmuCI | GTSAC | 1 cut(s) | 224 |
| NsbI | TGCGCA | 1 cut(s) | 92 |
| NspV | TTCGAA | 1 cut(s) | 233 |
| PleI | GAGTC | 1 cut(s) | 221 |
| PpsI | GAGTC | 1 cut(s) | 221 |
| Ppu21I | YACGTR | 1 cut(s) | 362 |
| Psp6I | CCWGG | 1 cut(s) | 108 |
| PspGI | CCWGG | 1 cut(s) | 108 |
| PspN4I | GGNNCC | 3 cut(s) | 144, 163, 353 |
| PspOMI | GGGCCC | 1 cut(s) | 351 |
| PspPI | GGNCC | 2 cut(s) | 351, 352 |
| PsuI | RGATCY | 1 cut(s) | 142 |
| SaqAI | TTAA | 1 cut(s) | 15 |
| Sau3AI | GATC | 1 cut(s) | 142 |
| Sau96I | GGNCC | 2 cut(s) | 351, 352 |
| SchI | GAGTC | 1 cut(s) | 221 |
| ScrFI | CCNGG | 1 cut(s) | 110 |
| SduI | GDGCHC | 1 cut(s) | 355 |
| SetI | ASST | 4 cut(s) | 72, 84, 333, 364 |
| SfaNI | GCATC | 2 cut(s) | 39, 198 |
| SfuI | TTCGAA | 1 cut(s) | 233 |
| SnaBI | TACGTA | 1 cut(s) | 362 |
| SsiI | CCGC | 4 cut(s) | 45, 222, 241, 252 |
| SspMI | CTAG | 1 cut(s) | 213 |
| StyD4I | CCNGG | 1 cut(s) | 108 |
| StyI | CCWWGG | 4 cut(s) | 157, 166, 195, 212 |
| TaiI | ACGT | 1 cut(s) | 364 |
| TaqI | TCGA | 2 cut(s) | 185, 233 |
| Tru1I | TTAA | 1 cut(s) | 15 |
| Tru9I | TTAA | 1 cut(s) | 15 |
| TseFI | GTSAC | 1 cut(s) | 224 |
| Tsp45I | GTSAC | 1 cut(s) | 224 |
| TspGWI | ACGGA | 1 cut(s) | 280 |
| XmaJI | CCTAGG | 1 cut(s) | 212 |
| XspI | CTAG | 1 cut(s) | 213 |