Rroxscaffold_1G00008670

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000057
Physical Location & Seq
Reverse (-)
10926823 .. 10930389
3567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00008670.1

Sequence Viewer

Length: 321 bp
ATGCTCCCCAAATCCCAACATCTCCCATTTCACAAAACCACACCGGCGAAAGAAGCGGCATGGCAGCATGATCTAGTCAATGGGAGAACTTATCACGAAGTGGGCATCTTTGAGCATCTCCTCAAAGAAACCGACGCGAGAAAGAATGCGTCCAAAGGCACCTACGTACTGGAAAACACTTTACGGTGGCCGCCCCGAACTATCAACCGGTTACCTATGATCCCTATTATCAAGGTGAAGAAGTTTTCCAGGATGAAGATGGTGAAGAGGAGAATGATGTCTCCGCATTTGAGCCGACAAGAAAGAAGAACTCGGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

106

Amino Acids

12.62

Weight (kDa)

11.23

Isoelectric Point (pI)

56.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000057)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00341 FvH4_2g18201 FvH4_2g24491 FvH4_2g24511 FvH4_3g22961 FvH4_3g33532 FvH4_3g33534 FvH4_4g05275 FvH4_4g12372 FvH4_5g20143 FvH4_7g12392
pyrus_communis pycom12g16120
rosa_chinensis RchiOBHm_Chr5g0023531 RchiOBHm_Chr6g0271201
rosa_laevigata RLG00000030639
rosa_multiflora Rmu_co8016136.1_g000001 Rmu_co8027180.1_g000001 Rmu_co8041016.1_g000001 Rmu_co8052812.1_g000001 Rmu_co8152468.1_g000001 Rmu_co8165796.1_g000001 Rmu_co8170214.1_g000001 Rmu_co8227433.1_g000001 Rmu_co8241379.1_g000001 Rmu_co8254305.1_g000001 Rmu_co8258647.1_g000001 Rmu_co8265535.1_g000002 Rmu_co8276845.1_g000001 Rmu_co8296303.1_g000001 Rmu_co8304827.1_g000001 Rmu_co8308345.1_g000001 Rmu_co8320493.1_g000002 Rmu_co8326955.1_g000001 Rmu_co8331007.1_g000001 Rmu_co8335625.1_g000001 Rmu_co8354465.1_g000001 Rmu_co8360201.1_g000001 Rmu_co8362075.1_g000002 Rmu_co8362991.1_g000001 Rmu_co8364567.1_g000001 Rmu_co8365273.1_g000001 Rmu_co8387267.1_g000001 Rmu_co8395251.1_g000002 Rmu_co8396677.1_g000001 Rmu_co8457659.1_g000001 Rmu_co8478117.1_g000001 Rmu_co8487163.1_g000001 Rmu_co8498169.1_g000001 Rmu_sc0000029.1_g000022 Rmu_sc0000063.1_g000007 Rmu_sc0000112.1_g000020 Rmu_sc0000146.1_g000006 Rmu_sc0000245.1_g000015 Rmu_sc0000276.1_g000036 Rmu_sc0000334.1_g000033 Rmu_sc0000334.1_g000034 Rmu_sc0000434.1_g000019 Rmu_sc0000441.1_g000118 Rmu_sc0000533.1_g000054 Rmu_sc0000533.1_g000055 Rmu_sc0000595.1_g000041 Rmu_sc0000663.1_g000019 Rmu_sc0000673.1_g000002 Rmu_sc0000698.1_g000102 Rmu_sc0000745.1_g000007 Rmu_sc0000768.1_g000018 Rmu_sc0000811.1_g000012 Rmu_sc0000826.1_g000009 Rmu_sc0000826.1_g000011 Rmu_sc0001051.1_g000042 Rmu_sc0001051.1_g000043 Rmu_sc0001148.1_g000014 Rmu_sc0001198.1_g000021 Rmu_sc0001227.1_g000008 Rmu_sc0001250.1_g000028 Rmu_sc0001395.1_g000019 Rmu_sc0001395.1_g000020 Rmu_sc0001396.1_g000002 Rmu_sc0001424.1_g000007 Rmu_sc0001455.1_g000025 Rmu_sc0001455.1_g000029 Rmu_sc0001499.1_g000036 Rmu_sc0001539.1_g000001 Rmu_sc0001562.1_g000027 Rmu_sc0001563.1_g000024 Rmu_sc0001719.1_g000022 Rmu_sc0001796.1_g000008 Rmu_sc0001884.1_g000022 Rmu_sc0001903.1_g000018 Rmu_sc0002041.1_g000012 Rmu_sc0002096.1_g000002 Rmu_sc0002126.1_g000027 Rmu_sc0002181.1_g000010 Rmu_sc0002259.1_g000004 Rmu_sc0002279.1_g000018 Rmu_sc0002312.1_g000060 Rmu_sc0002383.1_g000011 Rmu_sc0002401.1_g000012 Rmu_sc0002509.1_g000028 Rmu_sc0002569.1_g000008 Rmu_sc0002587.1_g000037 Rmu_sc0002665.1_g000006 Rmu_sc0002698.1_g000018 Rmu_sc0002724.1_g000002 Rmu_sc0002727.1_g000011 Rmu_sc0002927.1_g000055 Rmu_sc0002927.1_g000056 Rmu_sc0002943.1_g000009 Rmu_sc0002943.1_g000023 Rmu_sc0002998.1_g000001 Rmu_sc0003089.1_g000036 Rmu_sc0003127.1_g000022 Rmu_sc0003127.1_g000040 Rmu_sc0003207.1_g000010 Rmu_sc0003207.1_g000011 Rmu_sc0003275.1_g000034 Rmu_sc0003275.1_g000036 Rmu_sc0003337.1_g000047 Rmu_sc0003376.1_g000004 Rmu_sc0003522.1_g000024 Rmu_sc0003613.1_g000007 Rmu_sc0003708.1_g000026 Rmu_sc0003784.1_g000018 Rmu_sc0004079.1_g000005 Rmu_sc0004092.1_g000017 Rmu_sc0004168.1_g000058 Rmu_sc0004174.1_g000019 Rmu_sc0004199.1_g000049 Rmu_sc0004230.1_g000005 Rmu_sc0004283.1_g000021 Rmu_sc0004283.1_g000022 Rmu_sc0004314.1_g000017 Rmu_sc0004538.1_g000006 Rmu_sc0004542.1_g000011 Rmu_sc0004602.1_g000024 Rmu_sc0004641.1_g000006 Rmu_sc0004862.1_g000011 Rmu_sc0004941.1_g000043 Rmu_sc0005003.1_g000003 Rmu_sc0005112.1_g000018 Rmu_sc0005144.1_g000014 Rmu_sc0005266.1_g000014 Rmu_sc0005418.1_g000004 Rmu_sc0005627.1_g000006 Rmu_sc0005652.1_g000022 Rmu_sc0005747.1_g000032 Rmu_sc0005982.1_g000013 Rmu_sc0006050.1_g000017 Rmu_sc0006059.1_g000035 Rmu_sc0006128.1_g000002 Rmu_sc0006228.1_g000009 Rmu_sc0006243.1_g000009 Rmu_sc0006300.1_g000004 Rmu_sc0006463.1_g000007 Rmu_sc0006579.1_g000016 Rmu_sc0006595.1_g000011 Rmu_sc0006624.1_g000006 Rmu_sc0006624.1_g000007 Rmu_sc0006624.1_g000008 Rmu_sc0006674.1_g000025 Rmu_sc0006792.1_g000013 Rmu_sc0006872.1_g000022 Rmu_sc0006984.1_g000009 Rmu_sc0006984.1_g000010 Rmu_sc0006986.1_g000011 Rmu_sc0007016.1_g000001 Rmu_sc0007221.1_g000006 Rmu_sc0007222.1_g000005 Rmu_sc0007222.1_g000006 Rmu_sc0007251.1_g000001 Rmu_sc0007777.1_g000037 Rmu_sc0007826.1_g000018 Rmu_sc0008053.1_g000015 Rmu_sc0008502.1_g000006 Rmu_sc0008659.1_g000009 Rmu_sc0009045.1_g000009 Rmu_sc0009045.1_g000010 Rmu_sc0009060.1_g000012 Rmu_sc0009360.1_g000024 Rmu_sc0009431.1_g000001 Rmu_sc0009606.1_g000014 Rmu_sc0009774.1_g000001 Rmu_sc0009844.1_g000014 Rmu_sc0010313.1_g000001 Rmu_sc0010454.1_g000008 Rmu_sc0010543.1_g000016 Rmu_sc0010900.1_g000002 Rmu_sc0010925.1_g000009 Rmu_sc0011136.1_g000012 Rmu_sc0011534.1_g000004 Rmu_sc0011809.1_g000007 Rmu_sc0011917.1_g000005 Rmu_sc0012103.1_g000001 Rmu_sc0012287.1_g000001 Rmu_sc0012303.1_g000007 Rmu_sc0012344.1_g000009 Rmu_sc0012674.1_g000007 Rmu_sc0012686.1_g000001 Rmu_sc0012742.1_g000005 Rmu_sc0012746.1_g000026 Rmu_sc0012746.1_g000027 Rmu_sc0013419.1_g000008 Rmu_sc0014866.1_g000004 Rmu_sc0015325.1_g000011 Rmu_sc0015486.1_g000005 Rmu_sc0015651.1_g000002 Rmu_sc0015783.1_g000001 Rmu_sc0016387.1_g000009 Rmu_sc0017518.1_g000005 Rmu_sc0018480.1_g000001 Rmu_sc0018537.1_g000003 Rmu_sc0019200.1_g000002 Rmu_sc0019544.1_g000014 Rmu_sc0019544.1_g000015 Rmu_sc0019873.1_g000001 Rmu_sc0020392.1_g000001 Rmu_sc0020466.1_g000001 Rmu_sc0022008.1_g000001 Rmu_sc0022008.1_g000002 Rmu_sc0022274.1_g000001 Rmu_sc0023644.1_g000005 Rmu_sc0024657.1_g000001 Rmu_sc0024721.1_g000003 Rmu_sc0025790.1_g000001 Rmu_sc0026866.1_g000001 Rmu_sc0027363.1_g000002 Rmu_sc0027485.1_g000001 Rmu_sc0028394.1_g000001 Rmu_sc0033121.1_g000002 Rmu_sc0033838.1_g000001 Rmu_sc0035536.1_g000001 Rmu_sc0036296.1_g000001 Rmu_sc0036297.1_g000001 Rmu_sc0036298.1_g000001 Rmu_sc0036797.1_g000001 Rmu_sc0037010.1_g000001 Rmu_sc0037444.1_g000002 Rmu_sc0039012.1_g000002 Rmu_sc0040172.1_g000001 Rmu_sc0042083.1_g000001 Rmu_ssc0000018.1_g000001 Rmu_ssc0000171.1_g000032 Rmu_ssc0000213.1_g000064 Rmu_ssc0000213.1_g000096 Rmu_ssc0000262.1_g000017 Rmu_ssc0000295.1_g000036 Rmu_ssc0000343.1_g000008 Rmu_ssc0000398.1_g000003 Rmu_ssc0000400.1_g000085 Rmu_ssc0000438.1_g000007 Rmu_ssc0000478.1_g000003
rosa_roxburghii Rroxscaffold_166G00440710 Rroxscaffold_166G00440720 Rroxscaffold_174G00435300 Rroxscaffold_174G00435310 Rroxscaffold_177G00434290 Rroxscaffold_177G00434300 Rroxscaffold_177G00434310 Rroxscaffold_178G00437310 Rroxscaffold_1G00002500 Rroxscaffold_1G00004730 Rroxscaffold_1G00008660 Rroxscaffold_1G00008670 Rroxscaffold_1G00008680 Rroxscaffold_1G00008690 Rroxscaffold_1G00010260 Rroxscaffold_1G00015670 Rroxscaffold_1G00021140 Rroxscaffold_1G00024380 Rroxscaffold_1G00032420 Rroxscaffold_1G00050600 Rroxscaffold_1G00050610 Rroxscaffold_1G00055970 Rroxscaffold_1G00066220 Rroxscaffold_2G00085370 Rroxscaffold_2G00092350 Rroxscaffold_2G00092360 Rroxscaffold_2G00093350 Rroxscaffold_2G00097090 Rroxscaffold_2G00108040 Rroxscaffold_2G00109970 Rroxscaffold_2G00111470 Rroxscaffold_2G00111480 Rroxscaffold_2G00112000 Rroxscaffold_2G00112060 Rroxscaffold_2G00129160 Rroxscaffold_2G00132340 Rroxscaffold_2G00136720 Rroxscaffold_2G00152910 Rroxscaffold_3G00223160 Rroxscaffold_3G00223170 Rroxscaffold_3G00223520 Rroxscaffold_3G00230980 Rroxscaffold_3G00245030 Rroxscaffold_3G00259050 Rroxscaffold_3G00259060 Rroxscaffold_3G00259070 Rroxscaffold_3G00267470 Rroxscaffold_3G00272040 Rroxscaffold_3G00272440 Rroxscaffold_3G00272450 Rroxscaffold_4G00285860 Rroxscaffold_4G00285870 Rroxscaffold_4G00294380 Rroxscaffold_4G00294390 Rroxscaffold_4G00297890 Rroxscaffold_4G00298150 Rroxscaffold_4G00303090 Rroxscaffold_4G00306480 Rroxscaffold_4G00306680 Rroxscaffold_4G00310490 Rroxscaffold_4G00310630 Rroxscaffold_4G00310670 Rroxscaffold_4G00310680 Rroxscaffold_4G00310690 Rroxscaffold_4G00317930 Rroxscaffold_4G00320190 Rroxscaffold_4G00323370 Rroxscaffold_4G00323380 Rroxscaffold_4G00324200 Rroxscaffold_4G00331220 Rroxscaffold_5G00339230 Rroxscaffold_5G00341260 Rroxscaffold_5G00343120 Rroxscaffold_5G00345440 Rroxscaffold_5G00345450 Rroxscaffold_5G00353190 Rroxscaffold_5G00353420 Rroxscaffold_5G00356560 Rroxscaffold_5G00359790 Rroxscaffold_5G00381710 Rroxscaffold_5G00381720 Rroxscaffold_5G00386250 Rroxscaffold_5G00387520 Rroxscaffold_6G00390180 Rroxscaffold_6G00392810 Rroxscaffold_6G00401230 Rroxscaffold_6G00402640 Rroxscaffold_6G00405640 Rroxscaffold_6G00405650 Rroxscaffold_6G00405850 Rroxscaffold_6G00405860 Rroxscaffold_6G00412020 Rroxscaffold_6G00415690 Rroxscaffold_6G00415700 Rroxscaffold_7G00168110 Rroxscaffold_7G00178950 Rroxscaffold_7G00178960 Rroxscaffold_7G00193670 Rroxscaffold_7G00203020 Rroxscaffold_7G00203340 Rroxscaffold_7G00203550 Rroxscaffold_7G00207220 Rroxscaffold_7G00207250 Rroxscaffold_7G00207430 Rroxscaffold_7G00209310 Rroxscaffold_7G00211530 Rroxscaffold_7G00211540 Rroxscaffold_7G00214160 Rroxscaffold_7G00216040 Rroxscaffold_7G00216800
rosa_samantha Rh1AG276800 Rh1CG260400 Rh3CG366700
rosa_wichuraiana Rw1G001440 Rw3G029210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 158
AccII CGCG 1 cut(s) 137
AciI CCGC 3 cut(s) 56, 191, 284
AclWI GGATC 1 cut(s) 214
AcoI YGGCCR 1 cut(s) 188
AdeI CACNNNGTG 1 cut(s) 100
AfaI GTAC 1 cut(s) 168
AgeI ACCGGT 1 cut(s) 207
AjnI CCWGG 1 cut(s) 248
Alw26I GTCTC 1 cut(s) 285
AlwI GGATC 1 cut(s) 214
AoxI GGCC 2 cut(s) 188, 314
ApeKI GCWGC 1 cut(s) 64
AsiGI ACCGGT 1 cut(s) 207
AsuHPI GGTGA 2 cut(s) 247, 274
BanI GGYRCC 1 cut(s) 158
BbvI GCAGC 1 cut(s) 76
BccI CCATC 1 cut(s) 253
BciT130I CCWGG 1 cut(s) 250
BcoDI GTCTC 1 cut(s) 285
BfaI CTAG 1 cut(s) 74
BisI GCNGC 3 cut(s) 57, 65, 191
BlsI GCNGC 3 cut(s) 58, 66, 192
Bme1390I CCNGG 1 cut(s) 250
BmiI GGNNCC 1 cut(s) 160
BmrFI CCNGG 1 cut(s) 250
BmsI GCATC 2 cut(s) 114, 124
BsaAI YACGTR 1 cut(s) 166
BsaWI WCCGGW 1 cut(s) 207
Bse118I RCCGGY 2 cut(s) 43, 207
Bse1I ACTGG 1 cut(s) 174
BseBI CCWGG 1 cut(s) 250
BseGI GGATG 1 cut(s) 258
BseNI ACTGG 1 cut(s) 174
BseRI GAGGAG 2 cut(s) 110, 283
BseXI GCAGC 1 cut(s) 76
Bsh1236I CGCG 1 cut(s) 137
BshFI GGCC 2 cut(s) 190, 316
BshNI GGYRCC 1 cut(s) 158
BshTI ACCGGT 1 cut(s) 207
BsiSI CCGG 2 cut(s) 44, 208
BsmAI GTCTC 1 cut(s) 285
BsmI GAATGC 1 cut(s) 151
BsnI GGCC 2 cut(s) 190, 316
Bsp143I GATC 2 cut(s) 70, 219
BspACI CCGC 3 cut(s) 56, 191, 284
BspANI GGCC 2 cut(s) 190, 316
BspFNI CGCG 1 cut(s) 137
BspLI GGNNCC 1 cut(s) 160
BspPI GGATC 1 cut(s) 214
BspT107I GGYRCC 1 cut(s) 158
BsrFI RCCGGY 2 cut(s) 43, 207
BsrI ACTGG 1 cut(s) 174
BssAI RCCGGY 2 cut(s) 43, 207
BssMI GATC 2 cut(s) 70, 219
Bst2UI CCWGG 1 cut(s) 250
Bst4CI ACNGT 1 cut(s) 186
Bst6I CTCTTC 1 cut(s) 260
BstBAI YACGTR 1 cut(s) 166
BstEII GGTNACC 1 cut(s) 210
BstF5I GGATG 1 cut(s) 258
BstFNI CGCG 1 cut(s) 137
BstKTI GATC 2 cut(s) 73, 222
BstMAI GTCTC 1 cut(s) 285
BstMBI GATC 2 cut(s) 70, 219
BstMWI GCNNNNNNNGC 1 cut(s) 53
BstNI CCWGG 1 cut(s) 250
BstPI GGTNACC 1 cut(s) 210
BstSCI CCNGG 1 cut(s) 248
BstSNI TACGTA 1 cut(s) 166
BstUI CGCG 1 cut(s) 137
BstV1I GCAGC 1 cut(s) 76
BsuRI GGCC 2 cut(s) 190, 316
BtsCI GGATG 1 cut(s) 258
Cfr10I RCCGGY 2 cut(s) 43, 207
CseI GACGC 2 cut(s) 138, 143
Csp6I GTAC 1 cut(s) 167
CspAI ACCGGT 1 cut(s) 207
CviAII CATG 2 cut(s) 60, 68
CviJI RGCY 3 cut(s) 190, 294, 316
CviKI_1 RGCY 3 cut(s) 190, 294, 316
CviQI GTAC 1 cut(s) 167
DpnI GATC 2 cut(s) 72, 221
DpnII GATC 2 cut(s) 70, 219
DraIII CACNNNGTG 1 cut(s) 100
EaeI YGGCCR 1 cut(s) 188
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
Eco105I TACGTA 1 cut(s) 166
Eco91I GGTNACC 1 cut(s) 210
EcoO65I GGTNACC 1 cut(s) 210
EcoRII CCWGG 1 cut(s) 248
FaeI CATG 2 cut(s) 63, 71
FaiI YATR 3 cut(s) 61, 69, 218
FatI CATG 2 cut(s) 59, 67
Fnu4HI GCNGC 3 cut(s) 57, 65, 191
FokI GGATG 1 cut(s) 265
Fsp4HI GCNGC 3 cut(s) 57, 65, 191
FspBI CTAG 1 cut(s) 74
GluI GCNGC 3 cut(s) 57, 65, 191
HaeIII GGCC 2 cut(s) 190, 316
HapII CCGG 2 cut(s) 44, 208
HgaI GACGC 2 cut(s) 138, 143
Hin1II CATG 2 cut(s) 63, 71
HpaII CCGG 2 cut(s) 44, 208
HphI GGTGA 2 cut(s) 247, 274
Hpy188III TCNNGA 1 cut(s) 95
Hpy99I CGWCG 1 cut(s) 137
HpyCH4III ACNGT 1 cut(s) 186
HpyCH4IV ACGT 1 cut(s) 165
HpyF10VI GCNNNNNNNGC 1 cut(s) 53
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 2 cut(s) 63, 71
Kzo9I GATC 2 cut(s) 70, 219
LmnI GCTCC 1 cut(s) 9
LpnPI CCDG 5 cut(s) 57, 155, 221, 235, 262
Lsp1109I GCAGC 1 cut(s) 76
LweI GCATC 2 cut(s) 114, 124
MaeI CTAG 1 cut(s) 74
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 1 cut(s) 210
MalI GATC 2 cut(s) 72, 221
MboI GATC 2 cut(s) 70, 219
MboII GAAGA 4 cut(s) 250, 268, 277, 318
MnlI CCTC 2 cut(s) 131, 261
MspI CCGG 2 cut(s) 44, 208
MspR9I CCNGG 1 cut(s) 250
Mva1269I GAATGC 1 cut(s) 151
MvaI CCWGG 1 cut(s) 250
MvnI CGCG 1 cut(s) 137
MwoI GCNNNNNNNGC 1 cut(s) 53
NdeII GATC 2 cut(s) 70, 219
NlaIII CATG 2 cut(s) 63, 71
NlaIV GGNNCC 1 cut(s) 160
NmeAIII GCCGAG 1 cut(s) 292
PctI GAATGC 1 cut(s) 151
PfoI TCCNGGA 1 cut(s) 248
PinAI ACCGGT 1 cut(s) 207
PkrI GCNGC 3 cut(s) 58, 66, 192
Ppu21I YACGTR 1 cut(s) 166
Psp6I CCWGG 1 cut(s) 248
PspEI GGTNACC 1 cut(s) 210
PspGI CCWGG 1 cut(s) 248
PspN4I GGNNCC 1 cut(s) 160
RsaI GTAC 1 cut(s) 168
RsaNI GTAC 1 cut(s) 167
SatI GCNGC 3 cut(s) 57, 65, 191
Sau3AI GATC 2 cut(s) 70, 219
ScrFI CCNGG 1 cut(s) 250
SetI ASST 4 cut(s) 164, 168, 217, 237
SfaNI GCATC 2 cut(s) 114, 124
SgrAI CRCCGGYG 1 cut(s) 43
SnaBI TACGTA 1 cut(s) 166
SsiI CCGC 3 cut(s) 56, 191, 284
SspMI CTAG 1 cut(s) 74
StyD4I CCNGG 1 cut(s) 248
TaaI ACNGT 1 cut(s) 186
TaiI ACGT 1 cut(s) 168
TauI GCSGC 2 cut(s) 59, 193
TseI GCWGC 1 cut(s) 64
TspDTI ATGAA 1 cut(s) 269
XcmI CCANNNNNNNNNTGG 1 cut(s) 256
XspI CTAG 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.