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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 363 |
| AccI | GTMKAC | 1 cut(s) | 94 |
| AccII | CGCG | 2 cut(s) | 140, 515 |
| AciI | CCGC | 5 cut(s) | 33, 138, 219, 262, 513 |
| AcsI | RAATTY | 2 cut(s) | 164, 402 |
| AdeI | CACNNNGTG | 1 cut(s) | 297 |
| AgsI | TTSAA | 3 cut(s) | 76, 163, 391 |
| AjiI | CACGTC | 1 cut(s) | 240 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 437, 469 |
| AluBI | AGCT | 4 cut(s) | 14, 305, 397, 424 |
| AluI | AGCT | 4 cut(s) | 14, 305, 397, 424 |
| Alw21I | GWGCWC | 1 cut(s) | 88 |
| Ama87I | CYCGRG | 2 cut(s) | 195, 449 |
| AoxI | GGCC | 2 cut(s) | 118, 143 |
| ApeKI | GCWGC | 1 cut(s) | 394 |
| ApoI | RAATTY | 2 cut(s) | 164, 402 |
| AspLEI | GCGC | 1 cut(s) | 487 |
| AspS9I | GGNCC | 2 cut(s) | 119, 548 |
| AsuC2I | CCSGG | 3 cut(s) | 22, 113, 122 |
| AsuHPI | GGTGA | 2 cut(s) | 101, 433 |
| AsuII | TTCGAA | 1 cut(s) | 168 |
| AvaI | CYCGRG | 2 cut(s) | 195, 449 |
| AvaII | GGWCC | 1 cut(s) | 548 |
| AxyI | CCTNAGG | 1 cut(s) | 319 |
| BauI | CACGAG | 3 cut(s) | 87, 292, 488 |
| BbsI | GAAGAC | 1 cut(s) | 542 |
| Bbv12I | GWGCWC | 1 cut(s) | 88 |
| BbvI | GCAGC | 1 cut(s) | 406 |
| BccI | CCATC | 3 cut(s) | 110, 272, 523 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 491, 525 |
| BcnI | CCSGG | 3 cut(s) | 22, 113, 122 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 482 |
| BisI | GCNGC | 3 cut(s) | 263, 395, 513 |
| BlsI | GCNGC | 3 cut(s) | 264, 396, 514 |
| Bme1390I | CCNGG | 3 cut(s) | 22, 113, 122 |
| Bme18I | GGWCC | 1 cut(s) | 548 |
| BmeT110I | CYCGRG | 2 cut(s) | 195, 449 |
| BmgBI | CACGTC | 1 cut(s) | 240 |
| BmgT120I | GGNCC | 2 cut(s) | 119, 548 |
| BmiI | GGNNCC | 2 cut(s) | 235, 414 |
| BmrFI | CCNGG | 3 cut(s) | 22, 113, 122 |
| BmsI | GCATC | 6 cut(s) | 134, 321, 321, 355, 463, 510 |
| BpiI | GAAGAC | 1 cut(s) | 542 |
| Bpu14I | TTCGAA | 1 cut(s) | 168 |
| BpuMI | CCSGG | 3 cut(s) | 22, 113, 122 |
| BsaJI | CCNNGG | 3 cut(s) | 350, 408, 620 |
| Bse118I | RCCGGY | 1 cut(s) | 41 |
| Bse21I | CCTNAGG | 1 cut(s) | 319 |
| Bse3DI | GCAATG | 1 cut(s) | 52 |
| BseDI | CCNNGG | 3 cut(s) | 350, 408, 620 |
| BseGI | GGATG | 6 cut(s) | 121, 125, 187, 336, 346, 567 |
| BseMI | GCAATG | 1 cut(s) | 52 |
| BseXI | GCAGC | 1 cut(s) | 406 |
| Bsh1236I | CGCG | 2 cut(s) | 140, 515 |
| Bsh1285I | CGRYCG | 3 cut(s) | 21, 276, 563 |
| BshFI | GGCC | 2 cut(s) | 120, 145 |
| BsiEI | CGRYCG | 3 cut(s) | 21, 276, 563 |
| BsiHKAI | GWGCWC | 1 cut(s) | 88 |
| BsiHKCI | CYCGRG | 2 cut(s) | 195, 449 |
| BsiSI | CCGG | 4 cut(s) | 21, 42, 113, 122 |
| BslFI | GGGAC | 1 cut(s) | 369 |
| BsmFI | GGGAC | 1 cut(s) | 369 |
| BsmI | GAATGC | 1 cut(s) | 348 |
| BsnI | GGCC | 2 cut(s) | 120, 145 |
| BsoBI | CYCGRG | 2 cut(s) | 195, 449 |
| Bsp119I | TTCGAA | 1 cut(s) | 168 |
| Bsp1286I | GDGCHC | 1 cut(s) | 88 |
| Bsp19I | CCATGG | 1 cut(s) | 350 |
| BspACI | CCGC | 5 cut(s) | 33, 138, 219, 262, 513 |
| BspANI | GGCC | 2 cut(s) | 120, 145 |
| BspFNI | CGCG | 2 cut(s) | 140, 515 |
| BspLI | GGNNCC | 2 cut(s) | 235, 414 |
| BspQI | GCTCTTC | 1 cut(s) | 63 |
| BspT104I | TTCGAA | 1 cut(s) | 168 |
| BsrDI | GCAATG | 1 cut(s) | 52 |
| BsrFI | RCCGGY | 1 cut(s) | 41 |
| BssAI | RCCGGY | 1 cut(s) | 41 |
| BssECI | CCNNGG | 3 cut(s) | 350, 408, 620 |
| BssSI | CACGAG | 3 cut(s) | 87, 292, 488 |
| BssT1I | CCWWGG | 2 cut(s) | 350, 408 |
| Bst2BI | CACGAG | 3 cut(s) | 87, 292, 488 |
| Bst4CI | ACNGT | 1 cut(s) | 29 |
| Bst6I | CTCTTC | 1 cut(s) | 63 |
| BstBI | TTCGAA | 1 cut(s) | 168 |
| BstDEI | CTNAG | 1 cut(s) | 319 |
| BstDSI | CCRYGG | 2 cut(s) | 350, 620 |
| BstF5I | GGATG | 6 cut(s) | 121, 125, 187, 336, 346, 567 |
| BstFNI | CGCG | 2 cut(s) | 140, 515 |
| BstHHI | GCGC | 1 cut(s) | 487 |
| BstMCI | CGRYCG | 3 cut(s) | 21, 276, 563 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 421, 482 |
| BstSCI | CCNGG | 3 cut(s) | 20, 111, 120 |
| BstUI | CGCG | 2 cut(s) | 140, 515 |
| BstV1I | GCAGC | 1 cut(s) | 406 |
| BstV2I | GAAGAC | 1 cut(s) | 542 |
| Bsu36I | CCTNAGG | 1 cut(s) | 319 |
| BsuRI | GGCC | 2 cut(s) | 120, 145 |
| BtgI | CCRYGG | 2 cut(s) | 350, 620 |
| BtrI | CACGTC | 1 cut(s) | 240 |
| BtsCI | GGATG | 6 cut(s) | 121, 125, 187, 336, 346, 567 |
| CfoI | GCGC | 1 cut(s) | 487 |
| Cfr10I | RCCGGY | 1 cut(s) | 41 |
| Cfr13I | GGNCC | 2 cut(s) | 119, 548 |
| CseI | GACGC | 1 cut(s) | 164 |
| CviAII | CATG | 3 cut(s) | 258, 266, 351 |
| DdeI | CTNAG | 1 cut(s) | 319 |
| DraIII | CACNNNGTG | 1 cut(s) | 297 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 363 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 363 |
| Eam1104I | CTCTTC | 1 cut(s) | 63 |
| EarI | CTCTTC | 1 cut(s) | 63 |
| EciI | GGCGGA | 1 cut(s) | 48 |
| Eco130I | CCWWGG | 2 cut(s) | 350, 408 |
| Eco47I | GGWCC | 1 cut(s) | 548 |
| Eco81I | CCTNAGG | 1 cut(s) | 319 |
| Eco88I | CYCGRG | 2 cut(s) | 195, 449 |
| EcoT14I | CCWWGG | 2 cut(s) | 350, 408 |
| EcoT22I | ATGCAT | 1 cut(s) | 348 |
| ErhI | CCWWGG | 2 cut(s) | 350, 408 |
| FaeI | CATG | 3 cut(s) | 261, 269, 354 |
| FaiI | YATR | 8 cut(s) | 259, 267, 352, 360, 463, 469, 471, 599 |
| FaqI | GGGAC | 1 cut(s) | 369 |
| FatI | CATG | 3 cut(s) | 257, 265, 350 |
| FauNDI | CATATG | 1 cut(s) | 469 |
| FblI | GTMKAC | 1 cut(s) | 94 |
| Fnu4HI | GCNGC | 3 cut(s) | 263, 395, 513 |
| FokI | GGATG | 6 cut(s) | 112, 128, 174, 333, 343, 554 |
| Fsp4HI | GCNGC | 3 cut(s) | 263, 395, 513 |
| GlaI | GCGC | 1 cut(s) | 486 |
| GluI | GCNGC | 3 cut(s) | 263, 395, 513 |
| HaeIII | GGCC | 2 cut(s) | 120, 145 |
| HapII | CCGG | 4 cut(s) | 21, 42, 113, 122 |
| HgaI | GACGC | 1 cut(s) | 164 |
| HhaI | GCGC | 1 cut(s) | 487 |
| Hin1II | CATG | 3 cut(s) | 261, 269, 354 |
| Hin6I | GCGC | 1 cut(s) | 485 |
| HinP1I | GCGC | 1 cut(s) | 485 |
| HincII | GTYRAC | 1 cut(s) | 243 |
| HindII | GTYRAC | 1 cut(s) | 243 |
| HindIII | AAGCTT | 1 cut(s) | 12 |
| HinfI | GANTC | 4 cut(s) | 134, 269, 493, 609 |
| HpaII | CCGG | 4 cut(s) | 21, 42, 113, 122 |
| HphI | GGTGA | 2 cut(s) | 101, 433 |
| Hpy166II | GTNNAC | 2 cut(s) | 95, 243 |
| Hpy188I | TCNGA | 2 cut(s) | 63, 572 |
| Hpy188III | TCNNGA | 4 cut(s) | 292, 368, 497, 613 |
| Hpy8I | GTNNAC | 2 cut(s) | 95, 243 |
| Hpy99I | CGWCG | 2 cut(s) | 158, 503 |
| HpyAV | CCTTC | 1 cut(s) | 567 |
| HpyCH4III | ACNGT | 1 cut(s) | 29 |
| HpyCH4IV | ACGT | 2 cut(s) | 184, 239 |
| HpyCH4V | TGCA | 3 cut(s) | 334, 346, 590 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 421, 482 |
| HpyF3I | CTNAG | 1 cut(s) | 319 |
| HpySE526I | ACGT | 2 cut(s) | 184, 239 |
| Hsp92II | CATG | 3 cut(s) | 261, 269, 354 |
| HspAI | GCGC | 1 cut(s) | 485 |
| LguI | GCTCTTC | 1 cut(s) | 63 |
| LmnI | GCTCC | 1 cut(s) | 412 |
| LpnPI | CCDG | 5 cut(s) | 34, 55, 126, 135, 429 |
| Lsp1109I | GCAGC | 1 cut(s) | 406 |
| LweI | GCATC | 6 cut(s) | 134, 321, 321, 355, 463, 510 |
| MaeII | ACGT | 2 cut(s) | 184, 239 |
| MboII | GAAGA | 6 cut(s) | 50, 142, 351, 529, 547, 591 |
| MhlI | GDGCHC | 1 cut(s) | 88 |
| MluCI | AATT | 5 cut(s) | 51, 164, 402, 428, 600 |
| MlyI | GAGTC | 1 cut(s) | 143 |
| MnlI | CCTC | 2 cut(s) | 83, 288 |
| Mph1103I | ATGCAT | 1 cut(s) | 348 |
| MslI | CAYNNNNRTG | 1 cut(s) | 114 |
| MspA1I | CMGCKG | 1 cut(s) | 397 |
| MspI | CCGG | 4 cut(s) | 21, 42, 113, 122 |
| MspR9I | CCNGG | 3 cut(s) | 22, 113, 122 |
| Mva1269I | GAATGC | 1 cut(s) | 348 |
| MvnI | CGCG | 2 cut(s) | 140, 515 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 421, 482 |
| NciI | CCSGG | 3 cut(s) | 22, 113, 122 |
| NcoI | CCATGG | 1 cut(s) | 350 |
| NdeI | CATATG | 1 cut(s) | 469 |
| NlaIII | CATG | 3 cut(s) | 261, 269, 354 |
| NlaIV | GGNNCC | 2 cut(s) | 235, 414 |
| NsiI | ATGCAT | 1 cut(s) | 348 |
| NspV | TTCGAA | 1 cut(s) | 168 |
| PciSI | GCTCTTC | 1 cut(s) | 63 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 251, 495 |
| PctI | GAATGC | 1 cut(s) | 348 |
| PfeI | GAWTC | 3 cut(s) | 269, 493, 609 |
| PkrI | GCNGC | 3 cut(s) | 264, 396, 514 |
| PleI | GAGTC | 1 cut(s) | 142 |
| PpsI | GAGTC | 1 cut(s) | 142 |
| PspN4I | GGNNCC | 2 cut(s) | 235, 414 |
| PspPI | GGNCC | 2 cut(s) | 119, 548 |
| PvuII | CAGCTG | 1 cut(s) | 397 |
| RseI | CAYNNNNRTG | 1 cut(s) | 114 |
| SapI | GCTCTTC | 1 cut(s) | 63 |
| SatI | GCNGC | 3 cut(s) | 263, 395, 513 |
| Sau96I | GGNCC | 2 cut(s) | 119, 548 |
| SchI | GAGTC | 1 cut(s) | 143 |
| ScrFI | CCNGG | 3 cut(s) | 22, 113, 122 |
| SduI | GDGCHC | 1 cut(s) | 88 |
| SetI | ASST | 9 cut(s) | 16, 94, 187, 242, 299, 307, 399, 426, 553 |
| SfaNI | GCATC | 6 cut(s) | 134, 321, 321, 355, 463, 510 |
| SfuI | TTCGAA | 1 cut(s) | 168 |
| SinI | GGWCC | 1 cut(s) | 548 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 114 |
| Sse9I | AATT | 5 cut(s) | 51, 164, 402, 428, 600 |
| SsiI | CCGC | 5 cut(s) | 33, 138, 219, 262, 513 |
| SspI | AATATT | 1 cut(s) | 586 |
| StyD4I | CCNGG | 3 cut(s) | 20, 111, 120 |
| StyI | CCWWGG | 2 cut(s) | 350, 408 |
| TaaI | ACNGT | 1 cut(s) | 29 |
| TaiI | ACGT | 2 cut(s) | 187, 242 |
| TaqI | TCGA | 7 cut(s) | 17, 82, 153, 168, 276, 308, 498 |
| TaqII | GACCGA | 3 cut(s) | 262, 554, 577 |
| TasI | AATT | 5 cut(s) | 51, 164, 402, 428, 600 |
| TauI | GCSGC | 2 cut(s) | 265, 515 |
| TfiI | GAWTC | 3 cut(s) | 269, 493, 609 |
| TseI | GCWGC | 1 cut(s) | 394 |
| TspGWI | ACGGA | 1 cut(s) | 343 |
| VpaK11BI | GGWCC | 1 cut(s) | 548 |
| XapI | RAATTY | 2 cut(s) | 164, 402 |
| XmiI | GTMKAC | 1 cut(s) | 94 |
| Zsp2I | ATGCAT | 1 cut(s) | 348 |