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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 4 |
| AflII | CTTAAG | 1 cut(s) | 83 |
| AgsI | TTSAA | 1 cut(s) | 120 |
| ApoI | RAATTY | 1 cut(s) | 4 |
| AspS9I | GGNCC | 1 cut(s) | 142 |
| AvaII | GGWCC | 1 cut(s) | 142 |
| BaeGI | GKGCMC | 1 cut(s) | 113 |
| BfrI | CTTAAG | 1 cut(s) | 83 |
| Bme18I | GGWCC | 1 cut(s) | 142 |
| BmgT120I | GGNCC | 1 cut(s) | 142 |
| BseSI | GKGCMC | 1 cut(s) | 113 |
| Bsp1286I | GDGCHC | 1 cut(s) | 113 |
| BspTI | CTTAAG | 1 cut(s) | 83 |
| BstAFI | CTTAAG | 1 cut(s) | 83 |
| BstSLI | GKGCMC | 1 cut(s) | 113 |
| BtsIMutI | CAGTG | 1 cut(s) | 62 |
| Cfr13I | GGNCC | 1 cut(s) | 142 |
| Eco47I | GGWCC | 1 cut(s) | 142 |
| FaiI | YATR | 4 cut(s) | 115, 171, 173, 185 |
| HinfI | GANTC | 4 cut(s) | 47, 59, 67, 166 |
| Hpy188I | TCNGA | 2 cut(s) | 52, 165 |
| HpyCH4V | TGCA | 1 cut(s) | 178 |
| MaeIII | GTNAC | 1 cut(s) | 129 |
| MfeI | CAATTG | 1 cut(s) | 150 |
| MhlI | GDGCHC | 1 cut(s) | 113 |
| MluCI | AATT | 4 cut(s) | 4, 150, 157, 179 |
| MlyI | GAGTC | 3 cut(s) | 41, 53, 61 |
| MseI | TTAA | 2 cut(s) | 84, 156 |
| MspCI | CTTAAG | 1 cut(s) | 83 |
| MunI | CAATTG | 1 cut(s) | 150 |
| NmuCI | GTSAC | 1 cut(s) | 129 |
| PfeI | GAWTC | 1 cut(s) | 166 |
| PleI | GAGTC | 3 cut(s) | 41, 53, 61 |
| PpsI | GAGTC | 3 cut(s) | 41, 53, 61 |
| PspPI | GGNCC | 1 cut(s) | 142 |
| SaqAI | TTAA | 2 cut(s) | 84, 156 |
| Sau96I | GGNCC | 1 cut(s) | 142 |
| SchI | GAGTC | 3 cut(s) | 41, 53, 61 |
| SduI | GDGCHC | 1 cut(s) | 113 |
| SgeI | CNNG | 2 cut(s) | 56, 88 |
| SinI | GGWCC | 1 cut(s) | 142 |
| SmlI | CTYRAG | 1 cut(s) | 83 |
| SmoI | CTYRAG | 1 cut(s) | 83 |
| Sse9I | AATT | 4 cut(s) | 4, 150, 157, 179 |
| SspI | AATATT | 1 cut(s) | 126 |
| TasI | AATT | 4 cut(s) | 4, 150, 157, 179 |
| TfiI | GAWTC | 1 cut(s) | 166 |
| Tru1I | TTAA | 2 cut(s) | 84, 156 |
| Tru9I | TTAA | 2 cut(s) | 84, 156 |
| TscAI | CASTG | 1 cut(s) | 69 |
| TseFI | GTSAC | 1 cut(s) | 129 |
| Tsp45I | GTSAC | 1 cut(s) | 129 |
| TspDTI | ATGAA | 2 cut(s) | 17, 158 |
| TspRI | CASTG | 1 cut(s) | 69 |
| Vha464I | CTTAAG | 1 cut(s) | 83 |
| VpaK11BI | GGWCC | 1 cut(s) | 142 |
| XapI | RAATTY | 1 cut(s) | 4 |