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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 23 |
| AccII | CGCG | 2 cut(s) | 178, 848 |
| AciI | CCGC | 5 cut(s) | 326, 368, 503, 654, 846 |
| AclWI | GGATC | 1 cut(s) | 724 |
| AcoI | YGGCCR | 1 cut(s) | 708 |
| AfaI | GTAC | 2 cut(s) | 482, 699 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 528, 668, 763 |
| AgsI | TTSAA | 3 cut(s) | 39, 355, 721 |
| AluBI | AGCT | 3 cut(s) | 401, 535, 649 |
| AluI | AGCT | 3 cut(s) | 401, 535, 649 |
| Alw21I | GWGCWC | 3 cut(s) | 15, 176, 414 |
| Alw26I | GTCTC | 1 cut(s) | 105 |
| AlwI | GGATC | 1 cut(s) | 724 |
| Ama87I | CYCGRG | 1 cut(s) | 620 |
| AoxI | GGCC | 2 cut(s) | 555, 708 |
| ApeKI | GCWGC | 3 cut(s) | 371, 535, 538 |
| AspS9I | GGNCC | 1 cut(s) | 166 |
| AsuHPI | GGTGA | 2 cut(s) | 315, 517 |
| AvaI | CYCGRG | 1 cut(s) | 620 |
| AvaII | GGWCC | 1 cut(s) | 166 |
| Bbv12I | GWGCWC | 3 cut(s) | 15, 176, 414 |
| BbvI | GCAGC | 3 cut(s) | 358, 522, 550 |
| BccI | CCATC | 5 cut(s) | 280, 310, 337, 386, 634 |
| BclI | TGATCA | 1 cut(s) | 591 |
| BcoDI | GTCTC | 1 cut(s) | 105 |
| BfaI | CTAG | 2 cut(s) | 294, 300 |
| BfmI | CTRYAG | 1 cut(s) | 536 |
| BisI | GCNGC | 5 cut(s) | 369, 372, 536, 539, 846 |
| BlpI | GCTNAGC | 1 cut(s) | 657 |
| BlsI | GCNGC | 5 cut(s) | 370, 373, 537, 540, 847 |
| Bme18I | GGWCC | 1 cut(s) | 166 |
| BmeT110I | CYCGRG | 1 cut(s) | 620 |
| BmgT120I | GGNCC | 1 cut(s) | 166 |
| BmiI | GGNNCC | 2 cut(s) | 140, 167 |
| BmrI | ACTGGG | 2 cut(s) | 154, 728 |
| BmsI | GCATC | 2 cut(s) | 170, 207 |
| BmuI | ACTGGG | 2 cut(s) | 154, 728 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 657 |
| BsaJI | CCNNGG | 2 cut(s) | 77, 335 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 528, 668, 763 |
| Bse1I | ACTGG | 3 cut(s) | 134, 160, 734 |
| BseDI | CCNNGG | 2 cut(s) | 77, 335 |
| BseGI | GGATG | 2 cut(s) | 198, 329 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 528, 668, 763 |
| BseNI | ACTGG | 3 cut(s) | 134, 160, 734 |
| BseRI | GAGGAG | 2 cut(s) | 7, 659 |
| BseXI | GCAGC | 3 cut(s) | 358, 522, 550 |
| BsgI | GTGCAG | 1 cut(s) | 55 |
| Bsh1236I | CGCG | 2 cut(s) | 178, 848 |
| Bsh1285I | CGRYCG | 1 cut(s) | 378 |
| BshFI | GGCC | 2 cut(s) | 557, 710 |
| BsiEI | CGRYCG | 1 cut(s) | 378 |
| BsiHKAI | GWGCWC | 3 cut(s) | 15, 176, 414 |
| BsiHKCI | CYCGRG | 1 cut(s) | 620 |
| BslFI | GGGAC | 3 cut(s) | 69, 179, 353 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 528, 668, 763 |
| BsmAI | GTCTC | 1 cut(s) | 105 |
| BsmFI | GGGAC | 3 cut(s) | 69, 179, 353 |
| BsnI | GGCC | 2 cut(s) | 557, 710 |
| BsoBI | CYCGRG | 1 cut(s) | 620 |
| Bsp1286I | GDGCHC | 3 cut(s) | 15, 176, 414 |
| Bsp143I | GATC | 2 cut(s) | 591, 729 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 657 |
| Bsp68I | TCGCGA | 1 cut(s) | 178 |
| BspACI | CCGC | 5 cut(s) | 326, 368, 503, 654, 846 |
| BspANI | GGCC | 2 cut(s) | 557, 710 |
| BspFNI | CGCG | 2 cut(s) | 178, 848 |
| BspLI | GGNNCC | 2 cut(s) | 140, 167 |
| BspMAI | CTGCAG | 1 cut(s) | 540 |
| BspPI | GGATC | 1 cut(s) | 724 |
| BsrI | ACTGG | 3 cut(s) | 134, 160, 734 |
| BssECI | CCNNGG | 2 cut(s) | 77, 335 |
| BssMI | GATC | 2 cut(s) | 591, 729 |
| BssT1I | CCWWGG | 1 cut(s) | 335 |
| Bst4CI | ACNGT | 2 cut(s) | 81, 116 |
| Bst6I | CTCTTC | 1 cut(s) | 210 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 830 |
| BstC8I | GCNNGC | 6 cut(s) | 86, 90, 94, 123, 156, 176 |
| BstDEI | CTNAG | 3 cut(s) | 402, 429, 657 |
| BstDSI | CCRYGG | 1 cut(s) | 77 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 761 |
| BstF5I | GGATG | 2 cut(s) | 198, 329 |
| BstFNI | CGCG | 2 cut(s) | 178, 848 |
| BstKTI | GATC | 2 cut(s) | 594, 732 |
| BstMAI | GTCTC | 1 cut(s) | 105 |
| BstMBI | GATC | 2 cut(s) | 591, 729 |
| BstMCI | CGRYCG | 1 cut(s) | 378 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 180, 631, 830, 845 |
| BstNSI | RCATGY | 1 cut(s) | 817 |
| BstSFI | CTRYAG | 1 cut(s) | 536 |
| BstUI | CGCG | 2 cut(s) | 178, 848 |
| BstV1I | GCAGC | 3 cut(s) | 358, 522, 550 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 84, 261 |
| BsuRI | GGCC | 2 cut(s) | 557, 710 |
| BtgI | CCRYGG | 1 cut(s) | 77 |
| BtgZI | GCGATG | 1 cut(s) | 193 |
| BtsCI | GGATG | 2 cut(s) | 198, 329 |
| BtsI | GCAGTG | 1 cut(s) | 723 |
| BtsIMutI | CAGTG | 2 cut(s) | 167, 723 |
| BtuMI | TCGCGA | 1 cut(s) | 178 |
| Cac8I | GCNNGC | 6 cut(s) | 86, 90, 94, 123, 156, 176 |
| Cfr13I | GGNCC | 1 cut(s) | 166 |
| Csp6I | GTAC | 2 cut(s) | 481, 698 |
| CviAII | CATG | 3 cut(s) | 769, 805, 814 |
| CviQI | GTAC | 2 cut(s) | 481, 698 |
| DdeI | CTNAG | 3 cut(s) | 402, 429, 657 |
| DpnI | GATC | 2 cut(s) | 593, 731 |
| DpnII | GATC | 2 cut(s) | 591, 729 |
| EaeI | YGGCCR | 1 cut(s) | 708 |
| Eam1104I | CTCTTC | 1 cut(s) | 210 |
| EarI | CTCTTC | 1 cut(s) | 210 |
| Eco130I | CCWWGG | 1 cut(s) | 335 |
| Eco32I | GATATC | 1 cut(s) | 421 |
| Eco47I | GGWCC | 1 cut(s) | 166 |
| Eco88I | CYCGRG | 1 cut(s) | 620 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 761 |
| EcoO109I | RGGNCCY | 1 cut(s) | 166 |
| EcoRV | GATATC | 1 cut(s) | 421 |
| EcoT14I | CCWWGG | 1 cut(s) | 335 |
| ErhI | CCWWGG | 1 cut(s) | 335 |
| FaeI | CATG | 3 cut(s) | 772, 808, 817 |
| FaiI | YATR | 5 cut(s) | 770, 785, 806, 815, 831 |
| FaqI | GGGAC | 3 cut(s) | 69, 179, 353 |
| FatI | CATG | 3 cut(s) | 768, 804, 813 |
| FauI | CCCGC | 1 cut(s) | 661 |
| FbaI | TGATCA | 1 cut(s) | 591 |
| FblI | GTMKAC | 1 cut(s) | 23 |
| Fnu4HI | GCNGC | 5 cut(s) | 369, 372, 536, 539, 846 |
| FokI | GGATG | 2 cut(s) | 185, 316 |
| Fsp4HI | GCNGC | 5 cut(s) | 369, 372, 536, 539, 846 |
| FspBI | CTAG | 2 cut(s) | 294, 300 |
| GluI | GCNGC | 5 cut(s) | 369, 372, 536, 539, 846 |
| HaeIII | GGCC | 2 cut(s) | 557, 710 |
| Hin1II | CATG | 3 cut(s) | 772, 808, 817 |
| HinfI | GANTC | 4 cut(s) | 35, 320, 341, 689 |
| HphI | GGTGA | 2 cut(s) | 315, 517 |
| Hpy166II | GTNNAC | 4 cut(s) | 24, 483, 790, 811 |
| Hpy188III | TCNNGA | 3 cut(s) | 39, 177, 276 |
| Hpy8I | GTNNAC | 4 cut(s) | 24, 483, 790, 811 |
| HpyAV | CCTTC | 3 cut(s) | 300, 487, 493 |
| HpyCH4III | ACNGT | 2 cut(s) | 81, 116 |
| HpyCH4IV | ACGT | 1 cut(s) | 455 |
| HpyCH4V | TGCA | 9 cut(s) | 72, 96, 121, 183, 538, 801, 824, 833, 839 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 180, 631, 830, 845 |
| HpyF3I | CTNAG | 3 cut(s) | 402, 429, 657 |
| HpySE526I | ACGT | 1 cut(s) | 455 |
| Hsp92II | CATG | 3 cut(s) | 772, 808, 817 |
| Ksp22I | TGATCA | 1 cut(s) | 591 |
| Kzo9I | GATC | 2 cut(s) | 591, 729 |
| LmnI | GCTCC | 2 cut(s) | 138, 646 |
| Lsp1109I | GCAGC | 3 cut(s) | 358, 522, 550 |
| LweI | GCATC | 2 cut(s) | 170, 207 |
| MaeI | CTAG | 2 cut(s) | 294, 300 |
| MaeII | ACGT | 1 cut(s) | 455 |
| MaeIII | GTNAC | 2 cut(s) | 207, 321 |
| MalI | GATC | 2 cut(s) | 593, 731 |
| MboI | GATC | 2 cut(s) | 591, 729 |
| MboII | GAAGA | 2 cut(s) | 227, 698 |
| MhlI | GDGCHC | 3 cut(s) | 15, 176, 414 |
| MluCI | AATT | 3 cut(s) | 255, 280, 510 |
| MlyI | GAGTC | 2 cut(s) | 329, 335 |
| MmeI | TCCRAC | 1 cut(s) | 369 |
| MseI | TTAA | 3 cut(s) | 258, 452, 465 |
| MslI | CAYNNNNRTG | 1 cut(s) | 203 |
| MvnI | CGCG | 2 cut(s) | 178, 848 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 180, 631, 830, 845 |
| NdeII | GATC | 2 cut(s) | 591, 729 |
| NlaIII | CATG | 3 cut(s) | 772, 808, 817 |
| NlaIV | GGNNCC | 2 cut(s) | 140, 167 |
| NmuCI | GTSAC | 2 cut(s) | 207, 321 |
| NruI | TCGCGA | 1 cut(s) | 178 |
| NspI | RCATGY | 1 cut(s) | 817 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 26, 219, 759 |
| PfeI | GAWTC | 2 cut(s) | 35, 689 |
| PkrI | GCNGC | 5 cut(s) | 370, 373, 537, 540, 847 |
| PleI | GAGTC | 2 cut(s) | 328, 335 |
| PpsI | GAGTC | 2 cut(s) | 328, 335 |
| PpuMI | RGGWCCY | 1 cut(s) | 166 |
| Psp5II | RGGWCCY | 1 cut(s) | 166 |
| PspN4I | GGNNCC | 2 cut(s) | 140, 167 |
| PspPI | GGNCC | 1 cut(s) | 166 |
| PspPPI | RGGWCCY | 1 cut(s) | 166 |
| PstI | CTGCAG | 1 cut(s) | 540 |
| RruI | TCGCGA | 1 cut(s) | 178 |
| RsaI | GTAC | 2 cut(s) | 482, 699 |
| RsaNI | GTAC | 2 cut(s) | 481, 698 |
| RseI | CAYNNNNRTG | 1 cut(s) | 203 |
| SaqAI | TTAA | 3 cut(s) | 258, 452, 465 |
| SatI | GCNGC | 5 cut(s) | 369, 372, 536, 539, 846 |
| Sau3AI | GATC | 2 cut(s) | 591, 729 |
| Sau96I | GGNCC | 1 cut(s) | 166 |
| SchI | GAGTC | 2 cut(s) | 329, 335 |
| SduI | GDGCHC | 3 cut(s) | 15, 176, 414 |
| SfaNI | GCATC | 2 cut(s) | 170, 207 |
| SfcI | CTRYAG | 1 cut(s) | 536 |
| SinI | GGWCC | 1 cut(s) | 166 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 203 |
| Sse9I | AATT | 3 cut(s) | 255, 280, 510 |
| SsiI | CCGC | 5 cut(s) | 326, 368, 503, 654, 846 |
| SspMI | CTAG | 2 cut(s) | 294, 300 |
| StyI | CCWWGG | 1 cut(s) | 335 |
| TaaI | ACNGT | 2 cut(s) | 81, 116 |
| TaiI | ACGT | 1 cut(s) | 458 |
| TaqI | TCGA | 2 cut(s) | 222, 753 |
| TaqII | GACCGA | 1 cut(s) | 392 |
| TasI | AATT | 3 cut(s) | 255, 280, 510 |
| TauI | GCSGC | 2 cut(s) | 371, 848 |
| TfiI | GAWTC | 2 cut(s) | 35, 689 |
| Tru1I | TTAA | 3 cut(s) | 258, 452, 465 |
| Tru9I | TTAA | 3 cut(s) | 258, 452, 465 |
| TscAI | CASTG | 2 cut(s) | 167, 730 |
| TseFI | GTSAC | 2 cut(s) | 207, 321 |
| TseI | GCWGC | 3 cut(s) | 371, 535, 538 |
| Tsp45I | GTSAC | 2 cut(s) | 207, 321 |
| TspDTI | ATGAA | 2 cut(s) | 693, 756 |
| TspRI | CASTG | 2 cut(s) | 167, 730 |
| VpaK11BI | GGWCC | 1 cut(s) | 166 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 761 |
| XceI | RCATGY | 1 cut(s) | 817 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 258, 313 |
| XmiI | GTMKAC | 1 cut(s) | 23 |
| XspI | CTAG | 2 cut(s) | 294, 300 |