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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 5 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 278 |
| AciI | CCGC | 3 cut(s) | 456, 569, 801 |
| AclWI | GGATC | 2 cut(s) | 89, 320 |
| AcoI | YGGCCR | 2 cut(s) | 529, 802 |
| AcsI | RAATTY | 1 cut(s) | 302 |
| AdeI | CACNNNGTG | 1 cut(s) | 538 |
| AfaI | GTAC | 2 cut(s) | 150, 184 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 161, 278, 565, 587, 593, 661 |
| AgsI | TTSAA | 3 cut(s) | 79, 476, 790 |
| AjnI | CCWGG | 6 cut(s) | 318, 492, 504, 586, 592, 782 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 71, 103 |
| AluBI | AGCT | 1 cut(s) | 382 |
| AluI | AGCT | 1 cut(s) | 382 |
| Alw21I | GWGCWC | 1 cut(s) | 384 |
| AlwI | GGATC | 2 cut(s) | 89, 320 |
| Ama87I | CYCGRG | 1 cut(s) | 386 |
| AoxI | GGCC | 6 cut(s) | 63, 264, 529, 555, 589, 802 |
| ApeKI | GCWGC | 4 cut(s) | 177, 212, 315, 572 |
| ApoI | RAATTY | 1 cut(s) | 302 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 437, 469 |
| AspA2I | CCTAGG | 1 cut(s) | 109 |
| AspLEI | GCGC | 1 cut(s) | 6 |
| AspS9I | GGNCC | 3 cut(s) | 15, 556, 590 |
| AsuC2I | CCSGG | 2 cut(s) | 387, 388 |
| AsuHPI | GGTGA | 4 cut(s) | 176, 256, 494, 644 |
| AvaI | CYCGRG | 1 cut(s) | 386 |
| AvaII | GGWCC | 1 cut(s) | 15 |
| AvrII | CCTAGG | 1 cut(s) | 109 |
| BaeGI | GKGCMC | 1 cut(s) | 419 |
| BalI | TGGCCA | 1 cut(s) | 531 |
| BanII | GRGCYC | 1 cut(s) | 384 |
| BbsI | GAAGAC | 1 cut(s) | 759 |
| Bbv12I | GWGCWC | 1 cut(s) | 384 |
| BbvI | GCAGC | 4 cut(s) | 189, 224, 327, 559 |
| BccI | CCATC | 6 cut(s) | 113, 202, 350, 506, 567, 653 |
| BceAI | ACGGC | 6 cut(s) | 225, 291, 328, 582, 663, 783 |
| BciT130I | CCWGG | 6 cut(s) | 320, 494, 506, 588, 594, 784 |
| BclI | TGATCA | 1 cut(s) | 406 |
| BcnI | CCSGG | 2 cut(s) | 387, 388 |
| BfaI | CTAG | 2 cut(s) | 110, 779 |
| BisI | GCNGC | 6 cut(s) | 178, 213, 316, 570, 573, 802 |
| BlnI | CCTAGG | 1 cut(s) | 109 |
| BlsI | GCNGC | 6 cut(s) | 179, 214, 317, 571, 574, 803 |
| BmcAI | AGTACT | 1 cut(s) | 184 |
| Bme1390I | CCNGG | 8 cut(s) | 320, 387, 388, 494, 506, 588, 594, 784 |
| Bme18I | GGWCC | 1 cut(s) | 15 |
| BmeT110I | CYCGRG | 1 cut(s) | 386 |
| BmgT120I | GGNCC | 3 cut(s) | 15, 556, 590 |
| BmiI | GGNNCC | 1 cut(s) | 16 |
| BmrFI | CCNGG | 8 cut(s) | 320, 387, 388, 494, 506, 588, 594, 784 |
| BpiI | GAAGAC | 1 cut(s) | 759 |
| BpuMI | CCSGG | 2 cut(s) | 387, 388 |
| BsaBI | GATNNNNATC | 1 cut(s) | 428 |
| BsaJI | CCNNGG | 8 cut(s) | 66, 109, 207, 385, 386, 492, 505, 592 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 161, 278, 565, 587, 593, 661 |
| Bse1I | ACTGG | 2 cut(s) | 532, 805 |
| Bse3DI | GCAATG | 1 cut(s) | 803 |
| Bse8I | GATNNNNATC | 1 cut(s) | 428 |
| BseBI | CCWGG | 6 cut(s) | 320, 494, 506, 588, 594, 784 |
| BseDI | CCNNGG | 8 cut(s) | 66, 109, 207, 385, 386, 492, 505, 592 |
| BseGI | GGATG | 3 cut(s) | 105, 454, 625 |
| BseJI | GATNNNNATC | 1 cut(s) | 428 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 161, 278, 565, 587, 593, 661 |
| BseMI | GCAATG | 1 cut(s) | 803 |
| BseNI | ACTGG | 2 cut(s) | 532, 805 |
| BseRI | GAGGAG | 2 cut(s) | 570, 573 |
| BseSI | GKGCMC | 1 cut(s) | 419 |
| BseXI | GCAGC | 4 cut(s) | 189, 224, 327, 559 |
| BsgI | GTGCAG | 1 cut(s) | 558 |
| Bsh1285I | CGRYCG | 1 cut(s) | 155 |
| BshFI | GGCC | 6 cut(s) | 65, 266, 531, 557, 591, 804 |
| BsiEI | CGRYCG | 1 cut(s) | 155 |
| BsiHKAI | GWGCWC | 1 cut(s) | 384 |
| BsiHKCI | CYCGRG | 1 cut(s) | 386 |
| BsiSI | CCGG | 1 cut(s) | 387 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 161, 278, 565, 587, 593, 661 |
| BsmI | GAATGC | 2 cut(s) | 88, 163 |
| BsnI | GGCC | 6 cut(s) | 65, 266, 531, 557, 591, 804 |
| BsoBI | CYCGRG | 1 cut(s) | 386 |
| Bsp1286I | GDGCHC | 2 cut(s) | 384, 419 |
| Bsp143I | GATC | 5 cut(s) | 94, 325, 406, 423, 429 |
| BspACI | CCGC | 3 cut(s) | 456, 569, 801 |
| BspANI | GGCC | 6 cut(s) | 65, 266, 531, 557, 591, 804 |
| BspLI | GGNNCC | 1 cut(s) | 16 |
| BspPI | GGATC | 2 cut(s) | 89, 320 |
| BsrDI | GCAATG | 1 cut(s) | 803 |
| BsrI | ACTGG | 2 cut(s) | 532, 805 |
| BssECI | CCNNGG | 8 cut(s) | 66, 109, 207, 385, 386, 492, 505, 592 |
| BssMI | GATC | 5 cut(s) | 94, 325, 406, 423, 429 |
| BssT1I | CCWWGG | 2 cut(s) | 66, 109 |
| Bst2UI | CCWGG | 6 cut(s) | 320, 494, 506, 588, 594, 784 |
| Bst4CI | ACNGT | 1 cut(s) | 538 |
| Bst6I | CTCTTC | 1 cut(s) | 477 |
| BstC8I | GCNNGC | 1 cut(s) | 699 |
| BstDSI | CCRYGG | 1 cut(s) | 207 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 591 |
| BstF5I | GGATG | 3 cut(s) | 105, 454, 625 |
| BstHHI | GCGC | 1 cut(s) | 6 |
| BstKTI | GATC | 5 cut(s) | 97, 328, 409, 426, 432 |
| BstMBI | GATC | 5 cut(s) | 94, 325, 406, 423, 429 |
| BstMCI | CGRYCG | 1 cut(s) | 155 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 212, 315, 547 |
| BstNI | CCWGG | 6 cut(s) | 320, 494, 506, 588, 594, 784 |
| BstNSI | RCATGY | 1 cut(s) | 141 |
| BstSCI | CCNGG | 8 cut(s) | 318, 385, 386, 492, 504, 586, 592, 782 |
| BstSLI | GKGCMC | 1 cut(s) | 419 |
| BstV1I | GCAGC | 4 cut(s) | 189, 224, 327, 559 |
| BstV2I | GAAGAC | 1 cut(s) | 759 |
| BstX2I | RGATCY | 2 cut(s) | 94, 325 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 337 |
| BstYI | RGATCY | 2 cut(s) | 94, 325 |
| BsuRI | GGCC | 6 cut(s) | 65, 266, 531, 557, 591, 804 |
| BtgI | CCRYGG | 1 cut(s) | 207 |
| BtsCI | GGATG | 3 cut(s) | 105, 454, 625 |
| BtsI | GCAGTG | 1 cut(s) | 432 |
| BtsIMutI | CAGTG | 2 cut(s) | 432, 534 |
| Cac8I | GCNNGC | 1 cut(s) | 699 |
| CfoI | GCGC | 1 cut(s) | 6 |
| Cfr13I | GGNCC | 3 cut(s) | 15, 556, 590 |
| Cfr9I | CCCGGG | 1 cut(s) | 386 |
| Csp6I | GTAC | 2 cut(s) | 149, 183 |
| CviAII | CATG | 3 cut(s) | 40, 138, 694 |
| CviQI | GTAC | 2 cut(s) | 149, 183 |
| DpnI | GATC | 5 cut(s) | 96, 327, 408, 425, 431 |
| DpnII | GATC | 5 cut(s) | 94, 325, 406, 423, 429 |
| DraIII | CACNNNGTG | 1 cut(s) | 538 |
| EaeI | YGGCCR | 2 cut(s) | 529, 802 |
| Eam1104I | CTCTTC | 1 cut(s) | 477 |
| EarI | CTCTTC | 1 cut(s) | 477 |
| Ecl136II | GAGCTC | 1 cut(s) | 382 |
| Eco130I | CCWWGG | 2 cut(s) | 66, 109 |
| Eco24I | GRGCYC | 1 cut(s) | 384 |
| Eco47I | GGWCC | 1 cut(s) | 15 |
| Eco53kI | GAGCTC | 1 cut(s) | 382 |
| Eco88I | CYCGRG | 1 cut(s) | 386 |
| EcoICRI | GAGCTC | 1 cut(s) | 382 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 591 |
| EcoRII | CCWGG | 6 cut(s) | 318, 492, 504, 586, 592, 782 |
| EcoT14I | CCWWGG | 2 cut(s) | 66, 109 |
| EcoT22I | ATGCAT | 1 cut(s) | 88 |
| EcoT38I | GRGCYC | 1 cut(s) | 384 |
| ErhI | CCWWGG | 2 cut(s) | 66, 109 |
| FaeI | CATG | 3 cut(s) | 43, 141, 697 |
| FatI | CATG | 3 cut(s) | 39, 137, 693 |
| FbaI | TGATCA | 1 cut(s) | 406 |
| Fnu4HI | GCNGC | 6 cut(s) | 178, 213, 316, 570, 573, 802 |
| FokI | GGATG | 3 cut(s) | 92, 461, 632 |
| FriOI | GRGCYC | 1 cut(s) | 384 |
| Fsp4HI | GCNGC | 6 cut(s) | 178, 213, 316, 570, 573, 802 |
| FspBI | CTAG | 2 cut(s) | 110, 779 |
| FspI | TGCGCA | 1 cut(s) | 5 |
| GlaI | GCGC | 1 cut(s) | 5 |
| GluI | GCNGC | 6 cut(s) | 178, 213, 316, 570, 573, 802 |
| HaeIII | GGCC | 6 cut(s) | 65, 266, 531, 557, 591, 804 |
| HapII | CCGG | 1 cut(s) | 387 |
| HhaI | GCGC | 1 cut(s) | 6 |
| Hin1II | CATG | 3 cut(s) | 43, 141, 697 |
| Hin6I | GCGC | 1 cut(s) | 4 |
| HinP1I | GCGC | 1 cut(s) | 4 |
| HincII | GTYRAC | 1 cut(s) | 125 |
| HindII | GTYRAC | 1 cut(s) | 125 |
| HpaII | CCGG | 1 cut(s) | 387 |
| HphI | GGTGA | 4 cut(s) | 176, 256, 494, 644 |
| Hpy166II | GTNNAC | 4 cut(s) | 125, 219, 373, 502 |
| Hpy188I | TCNGA | 4 cut(s) | 300, 423, 564, 748 |
| Hpy188III | TCNNGA | 1 cut(s) | 427 |
| Hpy8I | GTNNAC | 4 cut(s) | 125, 219, 373, 502 |
| Hpy99I | CGWCG | 2 cut(s) | 314, 609 |
| HpyAV | CCTTC | 2 cut(s) | 439, 665 |
| HpyCH4III | ACNGT | 1 cut(s) | 538 |
| HpyCH4IV | ACGT | 1 cut(s) | 607 |
| HpyCH4V | TGCA | 6 cut(s) | 43, 86, 141, 550, 575, 796 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 212, 315, 547 |
| HpySE526I | ACGT | 1 cut(s) | 607 |
| Hsp92II | CATG | 3 cut(s) | 43, 141, 697 |
| HspAI | GCGC | 1 cut(s) | 4 |
| Ksp22I | TGATCA | 1 cut(s) | 406 |
| Kzo9I | GATC | 5 cut(s) | 94, 325, 406, 423, 429 |
| LmnI | GCTCC | 1 cut(s) | 387 |
| Lsp1109I | GCAGC | 4 cut(s) | 189, 224, 327, 559 |
| MaeI | CTAG | 2 cut(s) | 110, 779 |
| MaeII | ACGT | 1 cut(s) | 607 |
| MaeIII | GTNAC | 1 cut(s) | 164 |
| MalI | GATC | 5 cut(s) | 96, 327, 408, 425, 431 |
| MboI | GATC | 5 cut(s) | 94, 325, 406, 423, 429 |
| MboII | GAAGA | 3 cut(s) | 91, 464, 764 |
| MflI | RGATCY | 2 cut(s) | 94, 325 |
| MhlI | GDGCHC | 2 cut(s) | 384, 419 |
| MlsI | TGGCCA | 1 cut(s) | 531 |
| MluCI | AATT | 4 cut(s) | 53, 302, 391, 465 |
| MluNI | TGGCCA | 1 cut(s) | 531 |
| MnlI | CCTC | 7 cut(s) | 64, 588, 591, 594, 607, 665, 742 |
| Mox20I | TGGCCA | 1 cut(s) | 531 |
| Mph1103I | ATGCAT | 1 cut(s) | 88 |
| MscI | TGGCCA | 1 cut(s) | 531 |
| MslI | CAYNNNNRTG | 1 cut(s) | 657 |
| Msp20I | TGGCCA | 1 cut(s) | 531 |
| MspI | CCGG | 1 cut(s) | 387 |
| MspR9I | CCNGG | 8 cut(s) | 320, 387, 388, 494, 506, 588, 594, 784 |
| Mva1269I | GAATGC | 2 cut(s) | 88, 163 |
| MvaI | CCWGG | 6 cut(s) | 320, 494, 506, 588, 594, 784 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 212, 315, 547 |
| NciI | CCSGG | 2 cut(s) | 387, 388 |
| NdeII | GATC | 5 cut(s) | 94, 325, 406, 423, 429 |
| NlaIII | CATG | 3 cut(s) | 43, 141, 697 |
| NlaIV | GGNNCC | 1 cut(s) | 16 |
| NmuCI | GTSAC | 1 cut(s) | 164 |
| NsbI | TGCGCA | 1 cut(s) | 5 |
| NsiI | ATGCAT | 1 cut(s) | 88 |
| NspI | RCATGY | 1 cut(s) | 141 |
| PctI | GAATGC | 2 cut(s) | 88, 163 |
| PflFI | GACNNNGTC | 1 cut(s) | 170 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 278 |
| PkrI | GCNGC | 6 cut(s) | 179, 214, 317, 571, 574, 803 |
| Psp124BI | GAGCTC | 1 cut(s) | 384 |
| Psp6I | CCWGG | 6 cut(s) | 318, 492, 504, 586, 592, 782 |
| PspGI | CCWGG | 6 cut(s) | 318, 492, 504, 586, 592, 782 |
| PspN4I | GGNNCC | 1 cut(s) | 16 |
| PspPI | GGNCC | 3 cut(s) | 15, 556, 590 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 508, 540 |
| PsuI | RGATCY | 2 cut(s) | 94, 325 |
| PsyI | GACNNNGTC | 1 cut(s) | 170 |
| RsaI | GTAC | 2 cut(s) | 150, 184 |
| RsaNI | GTAC | 2 cut(s) | 149, 183 |
| RseI | CAYNNNNRTG | 1 cut(s) | 657 |
| SacI | GAGCTC | 1 cut(s) | 384 |
| SatI | GCNGC | 6 cut(s) | 178, 213, 316, 570, 573, 802 |
| Sau3AI | GATC | 5 cut(s) | 94, 325, 406, 423, 429 |
| Sau96I | GGNCC | 3 cut(s) | 15, 556, 590 |
| ScaI | AGTACT | 1 cut(s) | 184 |
| ScrFI | CCNGG | 8 cut(s) | 320, 387, 388, 494, 506, 588, 594, 784 |
| SduI | GDGCHC | 2 cut(s) | 384, 419 |
| SinI | GGWCC | 1 cut(s) | 15 |
| SmaI | CCCGGG | 1 cut(s) | 388 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 657 |
| Sse9I | AATT | 4 cut(s) | 53, 302, 391, 465 |
| SsiI | CCGC | 3 cut(s) | 456, 569, 801 |
| SspI | AATATT | 1 cut(s) | 487 |
| SspMI | CTAG | 2 cut(s) | 110, 779 |
| SstI | GAGCTC | 1 cut(s) | 384 |
| StyD4I | CCNGG | 8 cut(s) | 318, 385, 386, 492, 504, 586, 592, 782 |
| StyI | CCWWGG | 2 cut(s) | 66, 109 |
| TaaI | ACNGT | 1 cut(s) | 538 |
| TaiI | ACGT | 1 cut(s) | 610 |
| TaqI | TCGA | 1 cut(s) | 657 |
| TaqII | GACCGA | 1 cut(s) | 169 |
| TasI | AATT | 4 cut(s) | 53, 302, 391, 465 |
| TatI | WGTACW | 1 cut(s) | 182 |
| TauI | GCSGC | 2 cut(s) | 572, 804 |
| TscAI | CASTG | 2 cut(s) | 439, 541 |
| TseFI | GTSAC | 1 cut(s) | 164 |
| TseI | GCWGC | 4 cut(s) | 177, 212, 315, 572 |
| Tsp45I | GTSAC | 1 cut(s) | 164 |
| TspDTI | ATGAA | 1 cut(s) | 684 |
| TspMI | CCCGGG | 1 cut(s) | 386 |
| TspRI | CASTG | 2 cut(s) | 439, 541 |
| Tth111I | GACNNNGTC | 1 cut(s) | 170 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 278 |
| VpaK11BI | GGWCC | 1 cut(s) | 15 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 591 |
| XapI | RAATTY | 1 cut(s) | 302 |
| XceI | RCATGY | 1 cut(s) | 141 |
| XmaI | CCCGGG | 1 cut(s) | 386 |
| XmaJI | CCTAGG | 1 cut(s) | 109 |
| XspI | CTAG | 2 cut(s) | 110, 779 |
| ZrmI | AGTACT | 1 cut(s) | 184 |
| Zsp2I | ATGCAT | 1 cut(s) | 88 |