No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 824 |
| AarI | CACCTGC | 1 cut(s) | 919 |
| Acc36I | ACCTGC | 5 cut(s) | 415, 833, 919, 999, 1696 |
| AccB1I | GGYRCC | 1 cut(s) | 1257 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1384 |
| AccI | GTMKAC | 1 cut(s) | 459 |
| AccII | CGCG | 7 cut(s) | 578, 589, 646, 1029, 1031, 1637, 1724 |
| AclWI | GGATC | 1 cut(s) | 1751 |
| AcoI | YGGCCR | 1 cut(s) | 132 |
| AcuI | CTGAAG | 3 cut(s) | 900, 1293, 1698 |
| AcyI | GRCGYC | 1 cut(s) | 1258 |
| AfaI | GTAC | 4 cut(s) | 106, 947, 1629, 1695 |
| AfeI | AGCGCT | 2 cut(s) | 1113, 1217 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 397, 482, 993, 1384, 1432 |
| AgsI | TTSAA | 3 cut(s) | 250, 259, 1182 |
| AhlI | ACTAGT | 2 cut(s) | 454, 1072 |
| AjiI | CACGTC | 1 cut(s) | 463 |
| AjnI | CCWGG | 4 cut(s) | 91, 177, 950, 1479 |
| Alw21I | GWGCWC | 3 cut(s) | 644, 1410, 1470 |
| Alw26I | GTCTC | 5 cut(s) | 44, 747, 801, 1126, 1589 |
| AlwI | GGATC | 1 cut(s) | 1751 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1054, 1658 |
| Ama87I | CYCGRG | 1 cut(s) | 1454 |
| Aor51HI | AGCGCT | 2 cut(s) | 1113, 1217 |
| AoxI | GGCC | 6 cut(s) | 114, 132, 294, 732, 1051, 1489 |
| ApeKI | GCWGC | 8 cut(s) | 212, 371, 520, 836, 839, 1658, 1679, 1718 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 342, 374 |
| AspLEI | GCGC | 9 cut(s) | 589, 1008, 1029, 1031, 1033, 1114, 1218, 1260, 1275 |
| AspS9I | GGNCC | 5 cut(s) | 115, 175, 466, 732, 1051 |
| AsuC2I | CCSGG | 1 cut(s) | 229 |
| AsuHPI | GGTGA | 5 cut(s) | 292, 593, 618, 783, 1212 |
| AvaI | CYCGRG | 1 cut(s) | 1454 |
| AvaII | GGWCC | 2 cut(s) | 175, 466 |
| BanI | GGYRCC | 1 cut(s) | 1257 |
| BanII | GRGCYC | 1 cut(s) | 1470 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1677, 1709 |
| BauI | CACGAG | 1 cut(s) | 680 |
| BbsI | GAAGAC | 1 cut(s) | 1743 |
| Bbv12I | GWGCWC | 3 cut(s) | 644, 1410, 1470 |
| BbvI | GCAGC | 8 cut(s) | 224, 358, 532, 826, 848, 1645, 1691, 1730 |
| BccI | CCATC | 7 cut(s) | 162, 286, 581, 802, 869, 936, 1099 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 350, 384 |
| BciT130I | CCWGG | 4 cut(s) | 93, 179, 952, 1481 |
| BciVI | GTATCC | 1 cut(s) | 677 |
| BclI | TGATCA | 1 cut(s) | 1056 |
| BcnI | CCSGG | 1 cut(s) | 229 |
| BcoDI | GTCTC | 5 cut(s) | 44, 747, 801, 1126, 1589 |
| BcuI | ACTAGT | 2 cut(s) | 454, 1072 |
| BfaI | CTAG | 5 cut(s) | 455, 1034, 1073, 1188, 1524 |
| BfmI | CTRYAG | 3 cut(s) | 108, 840, 1731 |
| BfoI | RGCGCY | 3 cut(s) | 1115, 1219, 1261 |
| BfuAI | ACCTGC | 5 cut(s) | 415, 833, 919, 999, 1696 |
| BfuI | GTATCC | 1 cut(s) | 677 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1255 |
| Bme1390I | CCNGG | 5 cut(s) | 93, 179, 229, 952, 1481 |
| Bme18I | GGWCC | 2 cut(s) | 175, 466 |
| BmeT110I | CYCGRG | 1 cut(s) | 1454 |
| BmgBI | CACGTC | 1 cut(s) | 463 |
| BmgT120I | GGNCC | 5 cut(s) | 115, 175, 466, 732, 1051 |
| BmiI | GGNNCC | 7 cut(s) | 273, 468, 549, 712, 1259, 1416, 1557 |
| BmrFI | CCNGG | 5 cut(s) | 93, 179, 229, 952, 1481 |
| BmsI | GCATC | 4 cut(s) | 46, 46, 322, 1301 |
| BpiI | GAAGAC | 1 cut(s) | 1743 |
| BpmI | CTGGAG | 2 cut(s) | 186, 420 |
| Bpu10I | CCTNAGC | 3 cut(s) | 119, 1428, 1560 |
| BpuEI | CTTGAG | 1 cut(s) | 1647 |
| BpuMI | CCSGG | 1 cut(s) | 229 |
| Bsa29I | ATCGAT | 1 cut(s) | 290 |
| BsaAI | YACGTR | 1 cut(s) | 88 |
| BsaHI | GRCGYC | 1 cut(s) | 1258 |
| BsaI | GGTCTC | 1 cut(s) | 1126 |
| BsaJI | CCNNGG | 3 cut(s) | 267, 594, 951 |
| BsaWI | WCCGGW | 3 cut(s) | 307, 482, 1417 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 159, 189 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 397, 482, 993, 1384, 1432 |
| Bse118I | RCCGGY | 2 cut(s) | 130, 1432 |
| Bse1I | ACTGG | 4 cut(s) | 169, 437, 762, 1652 |
| Bse3DI | GCAATG | 3 cut(s) | 1474, 1646, 1700 |
| BseBI | CCWGG | 4 cut(s) | 93, 179, 952, 1481 |
| BseCI | ATCGAT | 1 cut(s) | 290 |
| BseDI | CCNNGG | 3 cut(s) | 267, 594, 951 |
| BseGI | GGATG | 3 cut(s) | 187, 199, 797 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 397, 482, 993, 1384, 1432 |
| BseMI | GCAATG | 3 cut(s) | 1474, 1646, 1700 |
| BseMII | CTCAG | 4 cut(s) | 58, 110, 1413, 1584 |
| BseNI | ACTGG | 4 cut(s) | 169, 437, 762, 1652 |
| BsePI | GCGCGC | 2 cut(s) | 1027, 1029 |
| BseRI | GAGGAG | 3 cut(s) | 32, 1418, 1568 |
| BseXI | GCAGC | 8 cut(s) | 224, 358, 532, 826, 848, 1645, 1691, 1730 |
| BseYI | CCCAGC | 1 cut(s) | 1324 |
| Bsh1236I | CGCG | 7 cut(s) | 578, 589, 646, 1029, 1031, 1637, 1724 |
| BshFI | GGCC | 6 cut(s) | 116, 134, 296, 734, 1053, 1491 |
| BshNI | GGYRCC | 1 cut(s) | 1257 |
| BshVI | ATCGAT | 1 cut(s) | 290 |
| BsiHKAI | GWGCWC | 3 cut(s) | 644, 1410, 1470 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1454 |
| BsiSI | CCGG | 6 cut(s) | 131, 228, 308, 483, 1418, 1433 |
| BslFI | GGGAC | 3 cut(s) | 970, 1343, 1603 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 397, 482, 993, 1384, 1432 |
| BsmAI | GTCTC | 5 cut(s) | 44, 747, 801, 1126, 1589 |
| BsmBI | CGTCTC | 1 cut(s) | 747 |
| BsmFI | GGGAC | 3 cut(s) | 970, 1343, 1603 |
| BsmI | GAATGC | 1 cut(s) | 665 |
| BsnI | GGCC | 6 cut(s) | 116, 134, 296, 734, 1053, 1491 |
| Bso31I | GGTCTC | 1 cut(s) | 1126 |
| BsoBI | CYCGRG | 1 cut(s) | 1454 |
| Bsp1286I | GDGCHC | 3 cut(s) | 644, 1410, 1470 |
| Bsp1407I | TGTACA | 1 cut(s) | 1693 |
| Bsp143I | GATC | 3 cut(s) | 1045, 1056, 1743 |
| Bsp68I | TCGCGA | 1 cut(s) | 646 |
| BspANI | GGCC | 6 cut(s) | 116, 134, 296, 734, 1053, 1491 |
| BspCNI | CTCAG | 4 cut(s) | 57, 111, 1412, 1585 |
| BspDI | ATCGAT | 1 cut(s) | 290 |
| BspFNI | CGCG | 7 cut(s) | 578, 589, 646, 1029, 1031, 1637, 1724 |
| BspLI | GGNNCC | 7 cut(s) | 273, 468, 549, 712, 1259, 1416, 1557 |
| BspMAI | CTGCAG | 1 cut(s) | 844 |
| BspMI | ACCTGC | 5 cut(s) | 415, 833, 919, 999, 1696 |
| BspPI | GGATC | 1 cut(s) | 1751 |
| BspT107I | GGYRCC | 1 cut(s) | 1257 |
| BspTNI | GGTCTC | 1 cut(s) | 1126 |
| BsrDI | GCAATG | 3 cut(s) | 1474, 1646, 1700 |
| BsrFI | RCCGGY | 2 cut(s) | 130, 1432 |
| BsrGI | TGTACA | 1 cut(s) | 1693 |
| BsrI | ACTGG | 4 cut(s) | 169, 437, 762, 1652 |
| BssAI | RCCGGY | 2 cut(s) | 130, 1432 |
| BssECI | CCNNGG | 3 cut(s) | 267, 594, 951 |
| BssHII | GCGCGC | 2 cut(s) | 1027, 1029 |
| BssMI | GATC | 3 cut(s) | 1045, 1056, 1743 |
| BssNI | GRCGYC | 1 cut(s) | 1258 |
| BssSI | CACGAG | 1 cut(s) | 680 |
| BssT1I | CCWWGG | 2 cut(s) | 267, 594 |
| Bst2BI | CACGAG | 1 cut(s) | 680 |
| Bst2UI | CCWGG | 4 cut(s) | 93, 179, 952, 1481 |
| Bst4CI | ACNGT | 4 cut(s) | 104, 109, 416, 787 |
| Bst6I | CTCTTC | 2 cut(s) | 183, 1680 |
| BstACI | GRCGYC | 1 cut(s) | 1258 |
| BstAUI | TGTACA | 1 cut(s) | 1693 |
| BstBAI | YACGTR | 1 cut(s) | 88 |
| BstC8I | GCNNGC | 7 cut(s) | 132, 591, 644, 1029, 1031, 1262, 1277 |
| BstDEI | CTNAG | 8 cut(s) | 44, 119, 894, 1209, 1399, 1428, 1560, 1593 |
| BstF5I | GGATG | 3 cut(s) | 187, 199, 797 |
| BstFNI | CGCG | 7 cut(s) | 578, 589, 646, 1029, 1031, 1637, 1724 |
| BstH2I | RGCGCY | 3 cut(s) | 1115, 1219, 1261 |
| BstHHI | GCGC | 9 cut(s) | 589, 1008, 1029, 1031, 1033, 1114, 1218, 1260, 1275 |
| BstKTI | GATC | 3 cut(s) | 1048, 1059, 1746 |
| BstMAI | GTCTC | 5 cut(s) | 44, 747, 801, 1126, 1589 |
| BstMBI | GATC | 3 cut(s) | 1045, 1056, 1743 |
| BstNI | CCWGG | 4 cut(s) | 93, 179, 952, 1481 |
| BstNSI | RCATGY | 1 cut(s) | 1268 |
| BstSCI | CCNGG | 5 cut(s) | 91, 177, 227, 950, 1479 |
| BstSFI | CTRYAG | 3 cut(s) | 108, 840, 1731 |
| BstUI | CGCG | 7 cut(s) | 578, 589, 646, 1029, 1031, 1637, 1724 |
| BstV1I | GCAGC | 8 cut(s) | 224, 358, 532, 826, 848, 1645, 1691, 1730 |
| BstV2I | GAAGAC | 1 cut(s) | 1743 |
| BstX2I | RGATCY | 1 cut(s) | 1743 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 729 |
| BstYI | RGATCY | 1 cut(s) | 1743 |
| Bsu15I | ATCGAT | 1 cut(s) | 290 |
| BsuI | GTATCC | 1 cut(s) | 677 |
| BsuRI | GGCC | 6 cut(s) | 116, 134, 296, 734, 1053, 1491 |
| BsuTUI | ATCGAT | 1 cut(s) | 290 |
| BtgZI | GCGATG | 3 cut(s) | 559, 661, 888 |
| BtrI | CACGTC | 1 cut(s) | 463 |
| BtsCI | GGATG | 3 cut(s) | 187, 199, 797 |
| BtsI | GCAGTG | 2 cut(s) | 975, 1364 |
| BtsIMutI | CAGTG | 3 cut(s) | 975, 1302, 1364 |
| BtuMI | TCGCGA | 1 cut(s) | 646 |
| BveI | ACCTGC | 5 cut(s) | 415, 833, 919, 999, 1696 |
| Cac8I | GCNNGC | 7 cut(s) | 132, 591, 644, 1029, 1031, 1262, 1277 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1054, 1658 |
| CfoI | GCGC | 9 cut(s) | 589, 1008, 1029, 1031, 1033, 1114, 1218, 1260, 1275 |
| Cfr10I | RCCGGY | 2 cut(s) | 130, 1432 |
| Cfr13I | GGNCC | 5 cut(s) | 115, 175, 466, 732, 1051 |
| ClaI | ATCGAT | 1 cut(s) | 290 |
| Csp6I | GTAC | 4 cut(s) | 105, 946, 1628, 1694 |
| CviAII | CATG | 4 cut(s) | 1265, 1286, 1582, 1618 |
| CviQI | GTAC | 4 cut(s) | 105, 946, 1628, 1694 |
| DdeI | CTNAG | 8 cut(s) | 44, 119, 894, 1209, 1399, 1428, 1560, 1593 |
| DinI | GGCGCC | 1 cut(s) | 1259 |
| DpnI | GATC | 3 cut(s) | 1047, 1058, 1745 |
| DpnII | GATC | 3 cut(s) | 1045, 1056, 1743 |
| EaeI | YGGCCR | 1 cut(s) | 132 |
| Eam1104I | CTCTTC | 2 cut(s) | 183, 1680 |
| EarI | CTCTTC | 2 cut(s) | 183, 1680 |
| Ecl136II | GAGCTC | 1 cut(s) | 1468 |
| Eco130I | CCWWGG | 2 cut(s) | 267, 594 |
| Eco147I | AGGCCT | 1 cut(s) | 1491 |
| Eco24I | GRGCYC | 1 cut(s) | 1470 |
| Eco31I | GGTCTC | 1 cut(s) | 1126 |
| Eco32I | GATATC | 1 cut(s) | 1543 |
| Eco47I | GGWCC | 2 cut(s) | 175, 466 |
| Eco47III | AGCGCT | 2 cut(s) | 1113, 1217 |
| Eco53kI | GAGCTC | 1 cut(s) | 1468 |
| Eco57I | CTGAAG | 3 cut(s) | 900, 1293, 1698 |
| Eco88I | CYCGRG | 1 cut(s) | 1454 |
| EcoICRI | GAGCTC | 1 cut(s) | 1468 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1051 |
| EcoRII | CCWGG | 4 cut(s) | 91, 177, 950, 1479 |
| EcoRV | GATATC | 1 cut(s) | 1543 |
| EcoT14I | CCWWGG | 2 cut(s) | 267, 594 |
| EcoT38I | GRGCYC | 1 cut(s) | 1470 |
| EgeI | GGCGCC | 1 cut(s) | 1259 |
| EheI | GGCGCC | 1 cut(s) | 1259 |
| ErhI | CCWWGG | 2 cut(s) | 267, 594 |
| Esp3I | CGTCTC | 1 cut(s) | 747 |
| FaeI | CATG | 4 cut(s) | 1268, 1289, 1585, 1621 |
| FaqI | GGGAC | 3 cut(s) | 970, 1343, 1603 |
| FatI | CATG | 4 cut(s) | 1264, 1285, 1581, 1617 |
| FauI | CCCGC | 3 cut(s) | 571, 1269, 1642 |
| FbaI | TGATCA | 1 cut(s) | 1056 |
| FblI | GTMKAC | 1 cut(s) | 459 |
| FokI | GGATG | 3 cut(s) | 194, 206, 784 |
| FriOI | GRGCYC | 1 cut(s) | 1470 |
| FspBI | CTAG | 5 cut(s) | 455, 1034, 1073, 1188, 1524 |
| GlaI | GCGC | 9 cut(s) | 588, 1007, 1028, 1030, 1032, 1113, 1217, 1259, 1274 |
| GsaI | CCCAGC | 1 cut(s) | 1328 |
| GsuI | CTGGAG | 2 cut(s) | 186, 420 |
| HaeII | RGCGCY | 3 cut(s) | 1115, 1219, 1261 |
| HaeIII | GGCC | 6 cut(s) | 116, 134, 296, 734, 1053, 1491 |
| HapII | CCGG | 6 cut(s) | 131, 228, 308, 483, 1418, 1433 |
| HhaI | GCGC | 9 cut(s) | 589, 1008, 1029, 1031, 1033, 1114, 1218, 1260, 1275 |
| Hin1I | GRCGYC | 1 cut(s) | 1258 |
| Hin1II | CATG | 4 cut(s) | 1268, 1289, 1585, 1621 |
| Hin6I | GCGC | 9 cut(s) | 587, 1006, 1027, 1029, 1031, 1112, 1216, 1258, 1273 |
| HinP1I | GCGC | 9 cut(s) | 587, 1006, 1027, 1029, 1031, 1112, 1216, 1258, 1273 |
| HincII | GTYRAC | 2 cut(s) | 460, 612 |
| HindII | GTYRAC | 2 cut(s) | 460, 612 |
| HinfI | GANTC | 5 cut(s) | 394, 486, 634, 809, 1163 |
| HpaII | CCGG | 6 cut(s) | 131, 228, 308, 483, 1418, 1433 |
| HphI | GGTGA | 5 cut(s) | 292, 593, 618, 783, 1212 |
| Hpy166II | GTNNAC | 6 cut(s) | 98, 460, 466, 612, 948, 1694 |
| Hpy188I | TCNGA | 7 cut(s) | 47, 552, 747, 852, 891, 1402, 1549 |
| Hpy188III | TCNNGA | 6 cut(s) | 75, 347, 361, 539, 645, 1664 |
| Hpy8I | GTNNAC | 6 cut(s) | 98, 460, 466, 612, 948, 1694 |
| Hpy99I | CGWCG | 4 cut(s) | 362, 464, 528, 742 |
| HpyAV | CCTTC | 4 cut(s) | 307, 481, 768, 1493 |
| HpyCH4III | ACNGT | 4 cut(s) | 104, 109, 416, 787 |
| HpyCH4IV | ACGT | 3 cut(s) | 87, 462, 1605 |
| HpyCH4V | TGCA | 6 cut(s) | 59, 424, 842, 1357, 1614, 1705 |
| HpyF3I | CTNAG | 8 cut(s) | 44, 119, 894, 1209, 1399, 1428, 1560, 1593 |
| HpySE526I | ACGT | 3 cut(s) | 87, 462, 1605 |
| Hsp92I | GRCGYC | 1 cut(s) | 1258 |
| Hsp92II | CATG | 4 cut(s) | 1268, 1289, 1585, 1621 |
| HspAI | GCGC | 9 cut(s) | 587, 1006, 1027, 1029, 1031, 1112, 1216, 1258, 1273 |
| KasI | GGCGCC | 1 cut(s) | 1257 |
| KroI | GCCGGC | 1 cut(s) | 130 |
| KroNI | GCCGGC | 1 cut(s) | 132 |
| Ksp22I | TGATCA | 1 cut(s) | 1056 |
| Kzo9I | GATC | 3 cut(s) | 1045, 1056, 1743 |
| LmnI | GCTCC | 6 cut(s) | 271, 439, 1213, 1405, 1444, 1555 |
| Lsp1109I | GCAGC | 8 cut(s) | 224, 358, 532, 826, 848, 1645, 1691, 1730 |
| LweI | GCATC | 4 cut(s) | 46, 46, 322, 1301 |
| MaeI | CTAG | 5 cut(s) | 455, 1034, 1073, 1188, 1524 |
| MaeII | ACGT | 3 cut(s) | 87, 462, 1605 |
| MaeIII | GTNAC | 3 cut(s) | 439, 999, 1708 |
| MalI | GATC | 3 cut(s) | 1047, 1058, 1745 |
| MboI | GATC | 3 cut(s) | 1045, 1056, 1743 |
| MboII | GAAGA | 7 cut(s) | 76, 200, 244, 247, 271, 1697, 1748 |
| MflI | RGATCY | 1 cut(s) | 1743 |
| MhlI | GDGCHC | 3 cut(s) | 644, 1410, 1470 |
| MluCI | AATT | 2 cut(s) | 603, 1568 |
| Mly113I | GGCGCC | 1 cut(s) | 1258 |
| MlyI | GAGTC | 1 cut(s) | 803 |
| MmeI | TCCRAC | 1 cut(s) | 837 |
| MroNI | GCCGGC | 1 cut(s) | 130 |
| MseI | TTAA | 2 cut(s) | 279, 408 |
| MspA1I | CMGCKG | 4 cut(s) | 446, 839, 1601, 1682 |
| MspI | CCGG | 6 cut(s) | 131, 228, 308, 483, 1418, 1433 |
| MspR9I | CCNGG | 5 cut(s) | 93, 179, 229, 952, 1481 |
| Mva1269I | GAATGC | 1 cut(s) | 665 |
| MvaI | CCWGG | 4 cut(s) | 93, 179, 952, 1481 |
| MvnI | CGCG | 7 cut(s) | 578, 589, 646, 1029, 1031, 1637, 1724 |
| NaeI | GCCGGC | 1 cut(s) | 132 |
| NarI | GGCGCC | 1 cut(s) | 1258 |
| NciI | CCSGG | 1 cut(s) | 229 |
| NdeII | GATC | 3 cut(s) | 1045, 1056, 1743 |
| NgoMIV | GCCGGC | 1 cut(s) | 130 |
| NlaIII | CATG | 4 cut(s) | 1268, 1289, 1585, 1621 |
| NlaIV | GGNNCC | 7 cut(s) | 273, 468, 549, 712, 1259, 1416, 1557 |
| NmeAIII | GCCGAG | 1 cut(s) | 1234 |
| NmuCI | GTSAC | 2 cut(s) | 439, 1708 |
| NruI | TCGCGA | 1 cut(s) | 646 |
| NspI | RCATGY | 1 cut(s) | 1268 |
| PaeR7I | CTCGAG | 1 cut(s) | 1454 |
| PaqCI | CACCTGC | 1 cut(s) | 919 |
| PauI | GCGCGC | 2 cut(s) | 1027, 1029 |
| PceI | AGGCCT | 1 cut(s) | 1491 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 354, 687, 1502 |
| PctI | GAATGC | 1 cut(s) | 665 |
| PdiI | GCCGGC | 1 cut(s) | 132 |
| PfeI | GAWTC | 4 cut(s) | 394, 486, 634, 1163 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1384 |
| PfoI | TCCNGGA | 1 cut(s) | 177 |
| PleI | GAGTC | 1 cut(s) | 803 |
| PluTI | GGCGCC | 1 cut(s) | 1261 |
| PpsI | GAGTC | 1 cut(s) | 803 |
| Ppu21I | YACGTR | 1 cut(s) | 88 |
| PsiI | TTATAA | 1 cut(s) | 824 |
| Psp124BI | GAGCTC | 1 cut(s) | 1470 |
| Psp6I | CCWGG | 4 cut(s) | 91, 177, 950, 1479 |
| PspFI | CCCAGC | 1 cut(s) | 1324 |
| PspGI | CCWGG | 4 cut(s) | 91, 177, 950, 1479 |
| PspN4I | GGNNCC | 7 cut(s) | 273, 468, 549, 712, 1259, 1416, 1557 |
| PspPI | GGNCC | 5 cut(s) | 115, 175, 466, 732, 1051 |
| PspXI | VCTCGAGB | 1 cut(s) | 1454 |
| PstI | CTGCAG | 1 cut(s) | 844 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1054, 1658 |
| PsuI | RGATCY | 1 cut(s) | 1743 |
| PteI | GCGCGC | 2 cut(s) | 1027, 1029 |
| PvuII | CAGCTG | 2 cut(s) | 839, 1601 |
| RruI | TCGCGA | 1 cut(s) | 646 |
| RsaI | GTAC | 4 cut(s) | 106, 947, 1629, 1695 |
| RsaNI | GTAC | 4 cut(s) | 105, 946, 1628, 1694 |
| SacI | GAGCTC | 1 cut(s) | 1470 |
| SalI | GTCGAC | 1 cut(s) | 458 |
| SaqAI | TTAA | 2 cut(s) | 279, 408 |
| Sau3AI | GATC | 3 cut(s) | 1045, 1056, 1743 |
| Sau96I | GGNCC | 5 cut(s) | 115, 175, 466, 732, 1051 |
| SchI | GAGTC | 1 cut(s) | 803 |
| ScrFI | CCNGG | 5 cut(s) | 93, 179, 229, 952, 1481 |
| SduI | GDGCHC | 3 cut(s) | 644, 1410, 1470 |
| SfaNI | GCATC | 4 cut(s) | 46, 46, 322, 1301 |
| SfcI | CTRYAG | 3 cut(s) | 108, 840, 1731 |
| SfoI | GGCGCC | 1 cut(s) | 1259 |
| Sfr274I | CTCGAG | 1 cut(s) | 1454 |
| SinI | GGWCC | 2 cut(s) | 175, 466 |
| SlaI | CTCGAG | 1 cut(s) | 1454 |
| SmlI | CTYRAG | 2 cut(s) | 1454, 1662 |
| SmoI | CTYRAG | 2 cut(s) | 1454, 1662 |
| SpeI | ACTAGT | 2 cut(s) | 454, 1072 |
| Sse9I | AATT | 2 cut(s) | 603, 1568 |
| SseBI | AGGCCT | 1 cut(s) | 1491 |
| SspDI | GGCGCC | 1 cut(s) | 1257 |
| SspMI | CTAG | 5 cut(s) | 455, 1034, 1073, 1188, 1524 |
| SstI | GAGCTC | 1 cut(s) | 1470 |
| StuI | AGGCCT | 1 cut(s) | 1491 |
| StyD4I | CCNGG | 5 cut(s) | 91, 177, 227, 950, 1479 |
| StyI | CCWWGG | 2 cut(s) | 267, 594 |
| TaaI | ACNGT | 4 cut(s) | 104, 109, 416, 787 |
| TaiI | ACGT | 3 cut(s) | 90, 465, 1608 |
| TaqI | TCGA | 7 cut(s) | 290, 360, 459, 690, 1234, 1455, 1566 |
| TasI | AATT | 2 cut(s) | 603, 1568 |
| TatI | WGTACW | 2 cut(s) | 1627, 1693 |
| TauI | GCSGC | 4 cut(s) | 116, 449, 452, 1724 |
| TfiI | GAWTC | 4 cut(s) | 394, 486, 634, 1163 |
| Tru1I | TTAA | 2 cut(s) | 279, 408 |
| Tru9I | TTAA | 2 cut(s) | 279, 408 |
| TscAI | CASTG | 3 cut(s) | 975, 1302, 1364 |
| TseFI | GTSAC | 2 cut(s) | 439, 1708 |
| TseI | GCWGC | 8 cut(s) | 212, 371, 520, 836, 839, 1658, 1679, 1718 |
| Tsp45I | GTSAC | 2 cut(s) | 439, 1708 |
| TspGWI | ACGGA | 3 cut(s) | 32, 541, 1511 |
| TspRI | CASTG | 3 cut(s) | 975, 1302, 1364 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1384 |
| VpaK11BI | GGWCC | 2 cut(s) | 175, 466 |
| XceI | RCATGY | 1 cut(s) | 1268 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 161, 726 |
| XhoI | CTCGAG | 1 cut(s) | 1454 |
| XmiI | GTMKAC | 1 cut(s) | 459 |
| XspI | CTAG | 5 cut(s) | 455, 1034, 1073, 1188, 1524 |