No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 2 cut(s) | 326, 392 |
| AciI | CCGC | 1 cut(s) | 369 |
| AcoI | YGGCCR | 1 cut(s) | 378 |
| AcuI | CTGAAG | 2 cut(s) | 465, 501 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 119, 188, 282 |
| AgsI | TTSAA | 2 cut(s) | 314, 467 |
| AjnI | CCWGG | 1 cut(s) | 380 |
| AleI | CACNNNNGTG | 1 cut(s) | 200 |
| AluBI | AGCT | 2 cut(s) | 322, 439 |
| AluI | AGCT | 2 cut(s) | 322, 439 |
| Alw26I | GTCTC | 2 cut(s) | 24, 443 |
| AoxI | GGCC | 3 cut(s) | 190, 273, 378 |
| AspLEI | GCGC | 2 cut(s) | 133, 429 |
| AspS9I | GGNCC | 1 cut(s) | 421 |
| AvaII | GGWCC | 1 cut(s) | 421 |
| BaeGI | GKGCMC | 1 cut(s) | 493 |
| BalI | TGGCCA | 1 cut(s) | 380 |
| BauI | CACGAG | 1 cut(s) | 200 |
| BciT130I | CCWGG | 1 cut(s) | 382 |
| BcoDI | GTCTC | 2 cut(s) | 24, 443 |
| BfaI | CTAG | 3 cut(s) | 453, 498, 517 |
| BfmI | CTRYAG | 1 cut(s) | 252 |
| BisI | GCNGC | 1 cut(s) | 369 |
| BlsI | GCNGC | 1 cut(s) | 370 |
| Bme1390I | CCNGG | 1 cut(s) | 382 |
| Bme18I | GGWCC | 1 cut(s) | 421 |
| BmgT120I | GGNCC | 1 cut(s) | 421 |
| BmiI | GGNNCC | 1 cut(s) | 59 |
| BmrFI | CCNGG | 1 cut(s) | 382 |
| BmrI | ACTGGG | 1 cut(s) | 73 |
| BmuI | ACTGGG | 1 cut(s) | 73 |
| BpmI | CTGGAG | 1 cut(s) | 165 |
| BsaJI | CCNNGG | 1 cut(s) | 381 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 163, 193, 294, 324 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 119, 188, 282 |
| Bse1I | ACTGG | 4 cut(s) | 53, 79, 182, 423 |
| Bse3DI | GCAATG | 1 cut(s) | 429 |
| BseBI | CCWGG | 1 cut(s) | 382 |
| BseDI | CCNNGG | 1 cut(s) | 381 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 119, 188, 282 |
| BseMI | GCAATG | 1 cut(s) | 429 |
| BseNI | ACTGG | 4 cut(s) | 53, 79, 182, 423 |
| BseSI | GKGCMC | 1 cut(s) | 493 |
| BseYI | CCCAGC | 1 cut(s) | 435 |
| BsgI | GTGCAG | 1 cut(s) | 224 |
| Bsh1236I | CGCG | 2 cut(s) | 326, 392 |
| Bsh1285I | CGRYCG | 1 cut(s) | 110 |
| BshFI | GGCC | 3 cut(s) | 192, 275, 380 |
| BsiEI | CGRYCG | 1 cut(s) | 110 |
| BslFI | GGGAC | 1 cut(s) | 98 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 119, 188, 282 |
| BsmAI | GTCTC | 2 cut(s) | 24, 443 |
| BsmFI | GGGAC | 1 cut(s) | 98 |
| BsnI | GGCC | 3 cut(s) | 192, 275, 380 |
| Bsp1286I | GDGCHC | 1 cut(s) | 493 |
| Bsp143I | GATC | 4 cut(s) | 107, 327, 400, 504 |
| Bsp68I | TCGCGA | 2 cut(s) | 326, 392 |
| BspACI | CCGC | 1 cut(s) | 369 |
| BspANI | GGCC | 3 cut(s) | 192, 275, 380 |
| BspFNI | CGCG | 2 cut(s) | 326, 392 |
| BspLI | GGNNCC | 1 cut(s) | 59 |
| BsrDI | GCAATG | 1 cut(s) | 429 |
| BsrI | ACTGG | 4 cut(s) | 53, 79, 182, 423 |
| BssECI | CCNNGG | 1 cut(s) | 381 |
| BssMI | GATC | 4 cut(s) | 107, 327, 400, 504 |
| BssSI | CACGAG | 1 cut(s) | 200 |
| Bst2BI | CACGAG | 1 cut(s) | 200 |
| Bst2UI | CCWGG | 1 cut(s) | 382 |
| Bst4CI | ACNGT | 2 cut(s) | 35, 310 |
| Bst6I | CTCTTC | 1 cut(s) | 441 |
| BstC8I | GCNNGC | 5 cut(s) | 10, 14, 75, 324, 441 |
| BstFNI | CGCG | 2 cut(s) | 326, 392 |
| BstHHI | GCGC | 2 cut(s) | 133, 429 |
| BstKTI | GATC | 4 cut(s) | 110, 330, 403, 507 |
| BstMAI | GTCTC | 2 cut(s) | 24, 443 |
| BstMBI | GATC | 4 cut(s) | 107, 327, 400, 504 |
| BstMCI | CGRYCG | 1 cut(s) | 110 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 248, 272, 377, 440 |
| BstNI | CCWGG | 1 cut(s) | 382 |
| BstNSI | RCATGY | 1 cut(s) | 497 |
| BstSCI | CCNGG | 1 cut(s) | 380 |
| BstSFI | CTRYAG | 1 cut(s) | 252 |
| BstSLI | GKGCMC | 1 cut(s) | 493 |
| BstUI | CGCG | 2 cut(s) | 326, 392 |
| BsuRI | GGCC | 3 cut(s) | 192, 275, 380 |
| BtsIMutI | CAGTG | 1 cut(s) | 86 |
| BtuMI | TCGCGA | 2 cut(s) | 326, 392 |
| Cac8I | GCNNGC | 5 cut(s) | 10, 14, 75, 324, 441 |
| CfoI | GCGC | 2 cut(s) | 133, 429 |
| Cfr13I | GGNCC | 1 cut(s) | 421 |
| CviAII | CATG | 2 cut(s) | 334, 494 |
| CviJI | RGCY | 8 cut(s) | 60, 192, 223, 275, 322, 368, 380, 439 |
| CviKI_1 | RGCY | 8 cut(s) | 60, 192, 223, 275, 322, 368, 380, 439 |
| DpnI | GATC | 4 cut(s) | 109, 329, 402, 506 |
| DpnII | GATC | 4 cut(s) | 107, 327, 400, 504 |
| EaeI | YGGCCR | 1 cut(s) | 378 |
| Eam1104I | CTCTTC | 1 cut(s) | 441 |
| EarI | CTCTTC | 1 cut(s) | 441 |
| Eco147I | AGGCCT | 1 cut(s) | 275 |
| Eco47I | GGWCC | 1 cut(s) | 421 |
| Eco57I | CTGAAG | 2 cut(s) | 465, 501 |
| EcoRII | CCWGG | 1 cut(s) | 380 |
| FaeI | CATG | 2 cut(s) | 337, 497 |
| FaiI | YATR | 8 cut(s) | 93, 212, 291, 335, 377, 410, 412, 495 |
| FaqI | GGGAC | 1 cut(s) | 98 |
| FatI | CATG | 2 cut(s) | 333, 493 |
| Fnu4HI | GCNGC | 1 cut(s) | 369 |
| Fsp4HI | GCNGC | 1 cut(s) | 369 |
| FspBI | CTAG | 3 cut(s) | 453, 498, 517 |
| GlaI | GCGC | 2 cut(s) | 132, 428 |
| GluI | GCNGC | 1 cut(s) | 369 |
| GsaI | CCCAGC | 1 cut(s) | 439 |
| GsuI | CTGGAG | 1 cut(s) | 165 |
| HaeIII | GGCC | 3 cut(s) | 192, 275, 380 |
| HhaI | GCGC | 2 cut(s) | 133, 429 |
| Hin1II | CATG | 2 cut(s) | 337, 497 |
| Hin6I | GCGC | 2 cut(s) | 131, 427 |
| HinP1I | GCGC | 2 cut(s) | 131, 427 |
| HincII | GTYRAC | 1 cut(s) | 159 |
| HindII | GTYRAC | 1 cut(s) | 159 |
| HpaI | GTTAAC | 1 cut(s) | 159 |
| Hpy166II | GTNNAC | 2 cut(s) | 153, 159 |
| Hpy188I | TCNGA | 2 cut(s) | 285, 400 |
| Hpy188III | TCNNGA | 5 cut(s) | 105, 325, 344, 356, 391 |
| Hpy8I | GTNNAC | 2 cut(s) | 153, 159 |
| HpyAV | CCTTC | 2 cut(s) | 286, 293 |
| HpyCH4III | ACNGT | 2 cut(s) | 35, 310 |
| HpyCH4IV | ACGT | 1 cut(s) | 155 |
| HpyCH4V | TGCA | 3 cut(s) | 40, 91, 205 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 248, 272, 377, 440 |
| HpySE526I | ACGT | 1 cut(s) | 155 |
| Hsp92II | CATG | 2 cut(s) | 337, 497 |
| HspAI | GCGC | 2 cut(s) | 131, 427 |
| KspAI | GTTAAC | 1 cut(s) | 159 |
| Kzo9I | GATC | 4 cut(s) | 107, 327, 400, 504 |
| LmnI | GCTCC | 1 cut(s) | 57 |
| MaeI | CTAG | 3 cut(s) | 453, 498, 517 |
| MaeII | ACGT | 1 cut(s) | 155 |
| MaeIII | GTNAC | 2 cut(s) | 184, 304 |
| MalI | GATC | 4 cut(s) | 109, 329, 402, 506 |
| MboI | GATC | 4 cut(s) | 107, 327, 400, 504 |
| MboII | GAAGA | 3 cut(s) | 93, 326, 458 |
| MhlI | GDGCHC | 1 cut(s) | 493 |
| MlsI | TGGCCA | 1 cut(s) | 380 |
| MluCI | AATT | 1 cut(s) | 462 |
| MluNI | TGGCCA | 1 cut(s) | 380 |
| MnlI | CCTC | 4 cut(s) | 189, 251, 253, 265 |
| Mox20I | TGGCCA | 1 cut(s) | 380 |
| MscI | TGGCCA | 1 cut(s) | 380 |
| MseI | TTAA | 1 cut(s) | 158 |
| MslI | CAYNNNNRTG | 1 cut(s) | 200 |
| Msp20I | TGGCCA | 1 cut(s) | 380 |
| MspA1I | CMGCKG | 1 cut(s) | 371 |
| MspR9I | CCNGG | 1 cut(s) | 382 |
| MvaI | CCWGG | 1 cut(s) | 382 |
| MvnI | CGCG | 2 cut(s) | 326, 392 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 248, 272, 377, 440 |
| NdeII | GATC | 4 cut(s) | 107, 327, 400, 504 |
| NlaIII | CATG | 2 cut(s) | 337, 497 |
| NlaIV | GGNNCC | 1 cut(s) | 59 |
| NmuCI | GTSAC | 1 cut(s) | 304 |
| NruI | TCGCGA | 2 cut(s) | 326, 392 |
| NspI | RCATGY | 1 cut(s) | 497 |
| OliI | CACNNNNGTG | 1 cut(s) | 200 |
| PceI | AGGCCT | 1 cut(s) | 275 |
| PkrI | GCNGC | 1 cut(s) | 370 |
| Ple19I | CGATCG | 1 cut(s) | 110 |
| Psp6I | CCWGG | 1 cut(s) | 380 |
| PspFI | CCCAGC | 1 cut(s) | 435 |
| PspGI | CCWGG | 1 cut(s) | 380 |
| PspN4I | GGNNCC | 1 cut(s) | 59 |
| PspPI | GGNCC | 1 cut(s) | 421 |
| PvuI | CGATCG | 1 cut(s) | 110 |
| RruI | TCGCGA | 2 cut(s) | 326, 392 |
| RseI | CAYNNNNRTG | 1 cut(s) | 200 |
| SaqAI | TTAA | 1 cut(s) | 158 |
| SatI | GCNGC | 1 cut(s) | 369 |
| Sau3AI | GATC | 4 cut(s) | 107, 327, 400, 504 |
| Sau96I | GGNCC | 1 cut(s) | 421 |
| ScrFI | CCNGG | 1 cut(s) | 382 |
| SduI | GDGCHC | 1 cut(s) | 493 |
| SetI | ASST | 6 cut(s) | 152, 158, 181, 324, 366, 441 |
| SfcI | CTRYAG | 1 cut(s) | 252 |
| SinI | GGWCC | 1 cut(s) | 421 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 200 |
| Sse9I | AATT | 1 cut(s) | 462 |
| SseBI | AGGCCT | 1 cut(s) | 275 |
| SsiI | CCGC | 1 cut(s) | 369 |
| SspI | AATATT | 1 cut(s) | 232 |
| SspMI | CTAG | 3 cut(s) | 453, 498, 517 |
| StuI | AGGCCT | 1 cut(s) | 275 |
| StyD4I | CCNGG | 1 cut(s) | 380 |
| TaaI | ACNGT | 2 cut(s) | 35, 310 |
| TaiI | ACGT | 1 cut(s) | 158 |
| TaqI | TCGA | 2 cut(s) | 106, 355 |
| TasI | AATT | 1 cut(s) | 462 |
| TauI | GCSGC | 1 cut(s) | 371 |
| Tru1I | TTAA | 1 cut(s) | 158 |
| Tru9I | TTAA | 1 cut(s) | 158 |
| TscAI | CASTG | 1 cut(s) | 86 |
| TseFI | GTSAC | 1 cut(s) | 304 |
| Tsp45I | GTSAC | 1 cut(s) | 304 |
| TspRI | CASTG | 1 cut(s) | 86 |
| VpaK11BI | GGWCC | 1 cut(s) | 421 |
| XceI | RCATGY | 1 cut(s) | 497 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 51 |
| XspI | CTAG | 3 cut(s) | 453, 498, 517 |