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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 763 |
| AccII | CGCG | 1 cut(s) | 349 |
| AccIII | TCCGGA | 1 cut(s) | 19 |
| AciI | CCGC | 8 cut(s) | 347, 575, 648, 696, 763, 792, 824, 832 |
| AcsI | RAATTY | 2 cut(s) | 318, 784 |
| AcuI | CTGAAG | 1 cut(s) | 648 |
| AcvI | CACGTG | 1 cut(s) | 518 |
| AfaI | GTAC | 1 cut(s) | 362 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 186, 353, 826 |
| AgeI | ACCGGT | 1 cut(s) | 44 |
| AgsI | TTSAA | 2 cut(s) | 304, 779 |
| AhlI | ACTAGT | 1 cut(s) | 547 |
| AjnI | CCWGG | 1 cut(s) | 687 |
| AleI | CACNNNNGTG | 1 cut(s) | 516 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 537, 569 |
| AluBI | AGCT | 2 cut(s) | 214, 310 |
| AluI | AGCT | 2 cut(s) | 214, 310 |
| Alw26I | GTCTC | 2 cut(s) | 19, 133 |
| Ama87I | CYCGRG | 1 cut(s) | 133 |
| Aor13HI | TCCGGA | 1 cut(s) | 19 |
| ApoI | RAATTY | 2 cut(s) | 318, 784 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 91, 123 |
| AsiGI | ACCGGT | 1 cut(s) | 44 |
| AspLEI | GCGC | 1 cut(s) | 449 |
| AspS9I | GGNCC | 2 cut(s) | 121, 829 |
| AsuHPI | GGTGA | 3 cut(s) | 59, 317, 676 |
| AvaI | CYCGRG | 1 cut(s) | 133 |
| AvaII | GGWCC | 2 cut(s) | 121, 829 |
| AxyI | CCTNAGG | 1 cut(s) | 73 |
| BauI | CACGAG | 1 cut(s) | 329 |
| BbrPI | CACGTG | 1 cut(s) | 518 |
| BccI | CCATC | 2 cut(s) | 586, 802 |
| BciT130I | CCWGG | 1 cut(s) | 689 |
| BciVI | GTATCC | 1 cut(s) | 782 |
| BcoDI | GTCTC | 2 cut(s) | 19, 133 |
| BcuI | ACTAGT | 1 cut(s) | 547 |
| BfaI | CTAG | 3 cut(s) | 125, 548, 678 |
| BfmI | CTRYAG | 1 cut(s) | 145 |
| BfuI | GTATCC | 1 cut(s) | 782 |
| BisI | GCNGC | 3 cut(s) | 696, 764, 792 |
| BlpI | GCTNAGC | 1 cut(s) | 311 |
| BlsI | GCNGC | 3 cut(s) | 697, 765, 793 |
| Bme1390I | CCNGG | 1 cut(s) | 689 |
| Bme18I | GGWCC | 2 cut(s) | 121, 829 |
| BmeT110I | CYCGRG | 1 cut(s) | 133 |
| BmgT120I | GGNCC | 2 cut(s) | 121, 829 |
| BmiI | GGNNCC | 3 cut(s) | 79, 139, 174 |
| BmrFI | CCNGG | 1 cut(s) | 689 |
| BmrI | ACTGGG | 1 cut(s) | 243 |
| BmsI | GCATC | 2 cut(s) | 661, 690 |
| BmuI | ACTGGG | 1 cut(s) | 243 |
| BoxI | GACNNNNGTC | 1 cut(s) | 147 |
| BpmI | CTGGAG | 2 cut(s) | 42, 481 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 311 |
| Bsa29I | ATCGAT | 1 cut(s) | 288 |
| BsaAI | YACGTR | 1 cut(s) | 518 |
| BsaJI | CCNNGG | 2 cut(s) | 132, 687 |
| BsaWI | WCCGGW | 2 cut(s) | 19, 44 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 479, 509 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 186, 353, 826 |
| Bse118I | RCCGGY | 1 cut(s) | 44 |
| Bse1I | ACTGG | 3 cut(s) | 59, 249, 498 |
| Bse21I | CCTNAGG | 1 cut(s) | 73 |
| BseAI | TCCGGA | 1 cut(s) | 19 |
| BseBI | CCWGG | 1 cut(s) | 689 |
| BseCI | ATCGAT | 1 cut(s) | 288 |
| BseDI | CCNNGG | 2 cut(s) | 132, 687 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 186, 353, 826 |
| BseMII | CTCAG | 1 cut(s) | 87 |
| BseNI | ACTGG | 3 cut(s) | 59, 249, 498 |
| BseRI | GAGGAG | 2 cut(s) | 121, 357 |
| BseYI | CCCAGC | 1 cut(s) | 651 |
| BsgI | GTGCAG | 1 cut(s) | 540 |
| Bsh1236I | CGCG | 1 cut(s) | 349 |
| Bsh1285I | CGRYCG | 1 cut(s) | 585 |
| BshTI | ACCGGT | 1 cut(s) | 44 |
| BshVI | ATCGAT | 1 cut(s) | 288 |
| BsiEI | CGRYCG | 1 cut(s) | 585 |
| BsiHKCI | CYCGRG | 1 cut(s) | 133 |
| BsiSI | CCGG | 2 cut(s) | 20, 45 |
| BslFI | GGGAC | 3 cut(s) | 47, 135, 417 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 186, 353, 826 |
| BsmAI | GTCTC | 2 cut(s) | 19, 133 |
| BsmFI | GGGAC | 3 cut(s) | 47, 135, 417 |
| BsmI | GAATGC | 1 cut(s) | 751 |
| BsoBI | CYCGRG | 1 cut(s) | 133 |
| Bsp13I | TCCGGA | 1 cut(s) | 19 |
| Bsp143I | GATC | 1 cut(s) | 506 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 311 |
| BspACI | CCGC | 8 cut(s) | 347, 575, 648, 696, 763, 792, 824, 832 |
| BspCNI | CTCAG | 1 cut(s) | 86 |
| BspDI | ATCGAT | 1 cut(s) | 288 |
| BspEI | TCCGGA | 1 cut(s) | 19 |
| BspFNI | CGCG | 1 cut(s) | 349 |
| BspLI | GGNNCC | 3 cut(s) | 79, 139, 174 |
| BspQI | GCTCTTC | 1 cut(s) | 753 |
| BsrBI | CCGCTC | 1 cut(s) | 763 |
| BsrFI | RCCGGY | 1 cut(s) | 44 |
| BsrI | ACTGG | 3 cut(s) | 59, 249, 498 |
| BssAI | RCCGGY | 1 cut(s) | 44 |
| BssECI | CCNNGG | 2 cut(s) | 132, 687 |
| BssMI | GATC | 1 cut(s) | 506 |
| BssSI | CACGAG | 1 cut(s) | 329 |
| Bst2BI | CACGAG | 1 cut(s) | 329 |
| Bst2UI | CCWGG | 1 cut(s) | 689 |
| Bst4CI | ACNGT | 3 cut(s) | 146, 166, 527 |
| Bst6I | CTCTTC | 1 cut(s) | 753 |
| BstBAI | YACGTR | 1 cut(s) | 518 |
| BstC8I | GCNNGC | 2 cut(s) | 667, 700 |
| BstDEI | CTNAG | 3 cut(s) | 73, 311, 767 |
| BstEII | GGTNACC | 1 cut(s) | 47 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 824 |
| BstFNI | CGCG | 1 cut(s) | 349 |
| BstHHI | GCGC | 1 cut(s) | 449 |
| BstKTI | GATC | 1 cut(s) | 509 |
| BstMAI | GTCTC | 2 cut(s) | 19, 133 |
| BstMBI | GATC | 1 cut(s) | 506 |
| BstMCI | CGRYCG | 1 cut(s) | 585 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 671 |
| BstNI | CCWGG | 1 cut(s) | 689 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 147 |
| BstPI | GGTNACC | 1 cut(s) | 47 |
| BstSCI | CCNGG | 1 cut(s) | 687 |
| BstSFI | CTRYAG | 1 cut(s) | 145 |
| BstUI | CGCG | 1 cut(s) | 349 |
| Bsu15I | ATCGAT | 1 cut(s) | 288 |
| Bsu36I | CCTNAGG | 1 cut(s) | 73 |
| BsuI | GTATCC | 1 cut(s) | 782 |
| BsuTUI | ATCGAT | 1 cut(s) | 288 |
| BtgZI | GCGATG | 1 cut(s) | 684 |
| BtsIMutI | CAGTG | 2 cut(s) | 95, 256 |
| Cac8I | GCNNGC | 2 cut(s) | 667, 700 |
| CfoI | GCGC | 1 cut(s) | 449 |
| Cfr10I | RCCGGY | 1 cut(s) | 44 |
| Cfr13I | GGNCC | 2 cut(s) | 121, 829 |
| ClaI | ATCGAT | 1 cut(s) | 288 |
| CseI | GACGC | 1 cut(s) | 663 |
| Csp6I | GTAC | 1 cut(s) | 361 |
| CspAI | ACCGGT | 1 cut(s) | 44 |
| CviAII | CATG | 2 cut(s) | 113, 800 |
| CviQI | GTAC | 1 cut(s) | 361 |
| DdeI | CTNAG | 3 cut(s) | 73, 311, 767 |
| DpnI | GATC | 1 cut(s) | 508 |
| DpnII | GATC | 1 cut(s) | 506 |
| Eam1104I | CTCTTC | 1 cut(s) | 753 |
| EarI | CTCTTC | 1 cut(s) | 753 |
| EciI | GGCGGA | 1 cut(s) | 564 |
| Eco47I | GGWCC | 2 cut(s) | 121, 829 |
| Eco57I | CTGAAG | 1 cut(s) | 648 |
| Eco72I | CACGTG | 1 cut(s) | 518 |
| Eco81I | CCTNAGG | 1 cut(s) | 73 |
| Eco88I | CYCGRG | 1 cut(s) | 133 |
| Eco91I | GGTNACC | 1 cut(s) | 47 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 824 |
| EcoO65I | GGTNACC | 1 cut(s) | 47 |
| EcoRI | GAATTC | 1 cut(s) | 784 |
| EcoRII | CCWGG | 1 cut(s) | 687 |
| FaeI | CATG | 2 cut(s) | 116, 803 |
| FaiI | YATR | 6 cut(s) | 114, 282, 294, 755, 796, 801 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 209, 241, 455, 487, 750, 782 |
| FaqI | GGGAC | 3 cut(s) | 47, 135, 417 |
| FatI | CATG | 2 cut(s) | 112, 799 |
| FauI | CCCGC | 1 cut(s) | 354 |
| Fnu4HI | GCNGC | 3 cut(s) | 696, 764, 792 |
| Fsp4HI | GCNGC | 3 cut(s) | 696, 764, 792 |
| FspBI | CTAG | 3 cut(s) | 125, 548, 678 |
| GlaI | GCGC | 1 cut(s) | 448 |
| GluI | GCNGC | 3 cut(s) | 696, 764, 792 |
| GsaI | CCCAGC | 1 cut(s) | 655 |
| GsuI | CTGGAG | 2 cut(s) | 42, 481 |
| HapII | CCGG | 2 cut(s) | 20, 45 |
| HgaI | GACGC | 1 cut(s) | 663 |
| HhaI | GCGC | 1 cut(s) | 449 |
| Hin1II | CATG | 2 cut(s) | 116, 803 |
| Hin6I | GCGC | 1 cut(s) | 447 |
| HinP1I | GCGC | 1 cut(s) | 447 |
| HincII | GTYRAC | 1 cut(s) | 110 |
| HindII | GTYRAC | 1 cut(s) | 110 |
| HindIII | AAGCTT | 1 cut(s) | 308 |
| HinfI | GANTC | 2 cut(s) | 68, 342 |
| HpaII | CCGG | 2 cut(s) | 20, 45 |
| HphI | GGTGA | 3 cut(s) | 59, 317, 676 |
| Hpy166II | GTNNAC | 3 cut(s) | 110, 374, 469 |
| Hpy188I | TCNGA | 1 cut(s) | 541 |
| Hpy188III | TCNNGA | 5 cut(s) | 20, 75, 135, 556, 740 |
| Hpy8I | GTNNAC | 3 cut(s) | 110, 374, 469 |
| Hpy99I | CGWCG | 3 cut(s) | 280, 354, 417 |
| HpyAV | CCTTC | 3 cut(s) | 45, 623, 785 |
| HpyCH4III | ACNGT | 3 cut(s) | 146, 166, 527 |
| HpyCH4IV | ACGT | 2 cut(s) | 278, 517 |
| HpyCH4V | TGCA | 1 cut(s) | 521 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 671 |
| HpyF3I | CTNAG | 3 cut(s) | 73, 311, 767 |
| HpySE526I | ACGT | 2 cut(s) | 278, 517 |
| Hsp92II | CATG | 2 cut(s) | 116, 803 |
| HspAI | GCGC | 1 cut(s) | 447 |
| Kpn2I | TCCGGA | 1 cut(s) | 19 |
| Kzo9I | GATC | 1 cut(s) | 506 |
| LguI | GCTCTTC | 1 cut(s) | 753 |
| LmnI | GCTCC | 2 cut(s) | 77, 137 |
| LweI | GCATC | 2 cut(s) | 661, 690 |
| MaeI | CTAG | 3 cut(s) | 125, 548, 678 |
| MaeII | ACGT | 2 cut(s) | 278, 517 |
| MaeIII | GTNAC | 5 cut(s) | 47, 154, 219, 500, 706 |
| MalI | GATC | 1 cut(s) | 508 |
| MbiI | CCGCTC | 1 cut(s) | 763 |
| MboI | GATC | 1 cut(s) | 506 |
| MboII | GAAGA | 3 cut(s) | 316, 770, 817 |
| MluCI | AATT | 3 cut(s) | 318, 475, 784 |
| MroI | TCCGGA | 1 cut(s) | 19 |
| MseI | TTAA | 3 cut(s) | 168, 443, 474 |
| MslI | CAYNNNNRTG | 1 cut(s) | 516 |
| MspA1I | CMGCKG | 1 cut(s) | 834 |
| MspI | CCGG | 2 cut(s) | 20, 45 |
| MspR9I | CCNGG | 1 cut(s) | 689 |
| Mva1269I | GAATGC | 1 cut(s) | 751 |
| MvaI | CCWGG | 1 cut(s) | 689 |
| MvnI | CGCG | 1 cut(s) | 349 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 671 |
| NdeII | GATC | 1 cut(s) | 506 |
| NlaIII | CATG | 2 cut(s) | 116, 803 |
| NlaIV | GGNNCC | 3 cut(s) | 79, 139, 174 |
| NmuCI | GTSAC | 3 cut(s) | 47, 154, 219 |
| OliI | CACNNNNGTG | 1 cut(s) | 516 |
| PciSI | GCTCTTC | 1 cut(s) | 753 |
| PctI | GAATGC | 1 cut(s) | 751 |
| PfeI | GAWTC | 2 cut(s) | 68, 342 |
| PinAI | ACCGGT | 1 cut(s) | 44 |
| PkrI | GCNGC | 3 cut(s) | 697, 765, 793 |
| PmaCI | CACGTG | 1 cut(s) | 518 |
| PmlI | CACGTG | 1 cut(s) | 518 |
| Ppu21I | YACGTR | 1 cut(s) | 518 |
| PshAI | GACNNNNGTC | 1 cut(s) | 147 |
| Psp6I | CCWGG | 1 cut(s) | 687 |
| PspCI | CACGTG | 1 cut(s) | 518 |
| PspEI | GGTNACC | 1 cut(s) | 47 |
| PspFI | CCCAGC | 1 cut(s) | 651 |
| PspGI | CCWGG | 1 cut(s) | 687 |
| PspN4I | GGNNCC | 3 cut(s) | 79, 139, 174 |
| PspPI | GGNCC | 2 cut(s) | 121, 829 |
| RsaI | GTAC | 1 cut(s) | 362 |
| RsaNI | GTAC | 1 cut(s) | 361 |
| RseI | CAYNNNNRTG | 1 cut(s) | 516 |
| SapI | GCTCTTC | 1 cut(s) | 753 |
| SaqAI | TTAA | 3 cut(s) | 168, 443, 474 |
| SatI | GCNGC | 3 cut(s) | 696, 764, 792 |
| Sau3AI | GATC | 1 cut(s) | 506 |
| Sau96I | GGNCC | 2 cut(s) | 121, 829 |
| ScrFI | CCNGG | 1 cut(s) | 689 |
| SfaNI | GCATC | 2 cut(s) | 661, 690 |
| SfcI | CTRYAG | 1 cut(s) | 145 |
| SinI | GGWCC | 2 cut(s) | 121, 829 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 516 |
| SmlI | CTYRAG | 1 cut(s) | 9 |
| SmoI | CTYRAG | 1 cut(s) | 9 |
| SpeI | ACTAGT | 1 cut(s) | 547 |
| Sse9I | AATT | 3 cut(s) | 318, 475, 784 |
| SsiI | CCGC | 8 cut(s) | 347, 575, 648, 696, 763, 792, 824, 832 |
| SspMI | CTAG | 3 cut(s) | 125, 548, 678 |
| StyD4I | CCNGG | 1 cut(s) | 687 |
| TaaI | ACNGT | 3 cut(s) | 146, 166, 527 |
| TaiI | ACGT | 2 cut(s) | 281, 520 |
| TaqI | TCGA | 3 cut(s) | 288, 581, 613 |
| TaqII | GACCGA | 2 cut(s) | 109, 599 |
| TasI | AATT | 3 cut(s) | 318, 475, 784 |
| TauI | GCSGC | 3 cut(s) | 698, 766, 794 |
| TfiI | GAWTC | 2 cut(s) | 68, 342 |
| Tru1I | TTAA | 3 cut(s) | 168, 443, 474 |
| Tru9I | TTAA | 3 cut(s) | 168, 443, 474 |
| TscAI | CASTG | 2 cut(s) | 95, 256 |
| TseFI | GTSAC | 3 cut(s) | 47, 154, 219 |
| Tsp45I | GTSAC | 3 cut(s) | 47, 154, 219 |
| TspRI | CASTG | 2 cut(s) | 95, 256 |
| VpaK11BI | GGWCC | 2 cut(s) | 121, 829 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 824 |
| XapI | RAATTY | 2 cut(s) | 318, 784 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 431 |
| XspI | CTAG | 3 cut(s) | 125, 548, 678 |