Uncharacterized protein K02A2.6-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 685 |
| Acc36I | ACCTGC | 2 cut(s) | 685, 1107 |
| AccB1I | GGYRCC | 1 cut(s) | 1021 |
| AccBSI | CCGCTC | 2 cut(s) | 941, 1324 |
| AccII | CGCG | 5 cut(s) | 482, 638, 916, 947, 1137 |
| AclWI | GGATC | 1 cut(s) | 764 |
| AcoI | YGGCCR | 1 cut(s) | 942 |
| AcsI | RAATTY | 1 cut(s) | 426 |
| AcuI | CTGAAG | 1 cut(s) | 23 |
| AcyI | GRCGYC | 1 cut(s) | 1022 |
| AfaI | GTAC | 4 cut(s) | 271, 602, 858, 1055 |
| AfeI | AGCGCT | 1 cut(s) | 723 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 667, 752, 1101 |
| AgsI | TTSAA | 2 cut(s) | 50, 415 |
| AjiI | CACGTC | 2 cut(s) | 128, 994 |
| AjnI | CCWGG | 3 cut(s) | 239, 342, 1155 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 461, 493 |
| AleI | CACNNNNGTG | 1 cut(s) | 185 |
| Alw21I | GWGCWC | 1 cut(s) | 821 |
| AlwI | GGATC | 1 cut(s) | 764 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1162 |
| Aor51HI | AGCGCT | 1 cut(s) | 723 |
| AoxI | GGCC | 6 cut(s) | 3, 30, 279, 459, 942, 1159 |
| ApeKI | GCWGC | 8 cut(s) | 142, 299, 377, 716, 719, 1111, 1226, 1251 |
| ApoI | RAATTY | 1 cut(s) | 426 |
| AspLEI | GCGC | 5 cut(s) | 511, 724, 1024, 1116, 1137 |
| AspS9I | GGNCC | 7 cut(s) | 4, 280, 340, 563, 780, 904, 1159 |
| AsuC2I | CCSGG | 1 cut(s) | 258 |
| AsuHPI | GGTGA | 4 cut(s) | 457, 559, 845, 1182 |
| AvaII | GGWCC | 4 cut(s) | 340, 563, 780, 904 |
| BanI | GGYRCC | 1 cut(s) | 1021 |
| BanII | GRGCYC | 1 cut(s) | 821 |
| Bbv12I | GWGCWC | 1 cut(s) | 821 |
| BbvI | GCAGC | 8 cut(s) | 154, 286, 364, 728, 731, 1123, 1213, 1263 |
| BccI | CCATC | 4 cut(s) | 160, 327, 538, 1043 |
| BceAI | ACGGC | 1 cut(s) | 729 |
| BciT130I | CCWGG | 3 cut(s) | 241, 344, 1157 |
| BclI | TGATCA | 1 cut(s) | 952 |
| BcnI | CCSGG | 1 cut(s) | 258 |
| BfaI | CTAG | 5 cut(s) | 407, 725, 734, 1142, 1151 |
| BfmI | CTRYAG | 2 cut(s) | 15, 1109 |
| BfoI | RGCGCY | 2 cut(s) | 725, 1025 |
| BfuAI | ACCTGC | 2 cut(s) | 685, 1107 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 506 |
| BglII | AGATCT | 1 cut(s) | 730 |
| BmcAI | AGTACT | 1 cut(s) | 602 |
| Bme1390I | CCNGG | 4 cut(s) | 241, 258, 344, 1157 |
| Bme18I | GGWCC | 4 cut(s) | 340, 563, 780, 904 |
| BmgBI | CACGTC | 2 cut(s) | 128, 994 |
| BmgT120I | GGNCC | 7 cut(s) | 4, 280, 340, 563, 780, 904, 1159 |
| BmiI | GGNNCC | 3 cut(s) | 123, 438, 1023 |
| BmrFI | CCNGG | 4 cut(s) | 241, 258, 344, 1157 |
| BmrI | ACTGGG | 2 cut(s) | 762, 1290 |
| BmsI | GCATC | 6 cut(s) | 159, 211, 211, 245, 487, 1295 |
| BmuI | ACTGGG | 2 cut(s) | 762, 1290 |
| Bpu10I | CCTNAGC | 2 cut(s) | 284, 1194 |
| BpuMI | CCSGG | 1 cut(s) | 258 |
| BsaAI | YACGTR | 2 cut(s) | 253, 492 |
| BsaHI | GRCGYC | 1 cut(s) | 1022 |
| BsaJI | CCNNGG | 5 cut(s) | 432, 846, 900, 945, 1156 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 667, 752, 1101 |
| Bse118I | RCCGGY | 2 cut(s) | 295, 1018 |
| Bse1I | ACTGG | 4 cut(s) | 757, 782, 1185, 1296 |
| BseBI | CCWGG | 3 cut(s) | 241, 344, 1157 |
| BseDI | CCNNGG | 5 cut(s) | 432, 846, 900, 945, 1156 |
| BseGI | GGATG | 4 cut(s) | 75, 236, 352, 1286 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 667, 752, 1101 |
| BseMII | CTCAG | 4 cut(s) | 275, 926, 1185, 1308 |
| BseNI | ACTGG | 4 cut(s) | 757, 782, 1185, 1296 |
| BseRI | GAGGAG | 2 cut(s) | 377, 1334 |
| BseX3I | CGGCCG | 1 cut(s) | 942 |
| BseXI | GCAGC | 8 cut(s) | 154, 286, 364, 728, 731, 1123, 1213, 1263 |
| Bsh1236I | CGCG | 5 cut(s) | 482, 638, 916, 947, 1137 |
| Bsh1285I | CGRYCG | 1 cut(s) | 945 |
| BshFI | GGCC | 6 cut(s) | 5, 32, 281, 461, 944, 1161 |
| BshNI | GGYRCC | 1 cut(s) | 1021 |
| BsiEI | CGRYCG | 1 cut(s) | 945 |
| BsiHKAI | GWGCWC | 1 cut(s) | 821 |
| BsiSI | CCGG | 3 cut(s) | 257, 296, 1019 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 667, 752, 1101 |
| BsmI | GAATGC | 1 cut(s) | 238 |
| BsnI | GGCC | 6 cut(s) | 5, 32, 281, 461, 944, 1161 |
| Bsp1286I | GDGCHC | 1 cut(s) | 821 |
| Bsp143I | GATC | 5 cut(s) | 531, 730, 756, 952, 972 |
| Bsp68I | TCGCGA | 2 cut(s) | 482, 916 |
| BspANI | GGCC | 6 cut(s) | 5, 32, 281, 461, 944, 1161 |
| BspCNI | CTCAG | 4 cut(s) | 276, 927, 1186, 1309 |
| BspFNI | CGCG | 5 cut(s) | 482, 638, 916, 947, 1137 |
| BspHI | TCATGA | 1 cut(s) | 153 |
| BspLI | GGNNCC | 3 cut(s) | 123, 438, 1023 |
| BspMAI | CTGCAG | 1 cut(s) | 1113 |
| BspMI | ACCTGC | 2 cut(s) | 685, 1107 |
| BspPI | GGATC | 1 cut(s) | 764 |
| BspT107I | GGYRCC | 1 cut(s) | 1021 |
| BsrBI | CCGCTC | 2 cut(s) | 941, 1324 |
| BsrFI | RCCGGY | 2 cut(s) | 295, 1018 |
| BsrI | ACTGG | 4 cut(s) | 757, 782, 1185, 1296 |
| BssAI | RCCGGY | 2 cut(s) | 295, 1018 |
| BssECI | CCNNGG | 5 cut(s) | 432, 846, 900, 945, 1156 |
| BssMI | GATC | 5 cut(s) | 531, 730, 756, 952, 972 |
| BssNI | GRCGYC | 1 cut(s) | 1022 |
| BssT1I | CCWWGG | 1 cut(s) | 432 |
| Bst2UI | CCWGG | 3 cut(s) | 241, 344, 1157 |
| Bst4CI | ACNGT | 3 cut(s) | 274, 644, 1236 |
| Bst6I | CTCTTC | 5 cut(s) | 327, 354, 921, 960, 1094 |
| BstACI | GRCGYC | 1 cut(s) | 1022 |
| BstBAI | YACGTR | 2 cut(s) | 253, 492 |
| BstC8I | GCNNGC | 3 cut(s) | 843, 914, 1139 |
| BstDEI | CTNAG | 5 cut(s) | 209, 284, 935, 1194, 1317 |
| BstDSI | CCRYGG | 3 cut(s) | 846, 900, 945 |
| BstF5I | GGATG | 4 cut(s) | 75, 236, 352, 1286 |
| BstFNI | CGCG | 5 cut(s) | 482, 638, 916, 947, 1137 |
| BstH2I | RGCGCY | 2 cut(s) | 725, 1025 |
| BstHHI | GCGC | 5 cut(s) | 511, 724, 1024, 1116, 1137 |
| BstKTI | GATC | 5 cut(s) | 534, 733, 759, 955, 975 |
| BstMBI | GATC | 5 cut(s) | 531, 730, 756, 952, 972 |
| BstMCI | CGRYCG | 1 cut(s) | 945 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 506, 947, 1003, 1028, 1226 |
| BstNI | CCWGG | 3 cut(s) | 241, 344, 1157 |
| BstSCI | CCNGG | 4 cut(s) | 239, 256, 342, 1155 |
| BstSFI | CTRYAG | 2 cut(s) | 15, 1109 |
| BstSNI | TACGTA | 1 cut(s) | 492 |
| BstUI | CGCG | 5 cut(s) | 482, 638, 916, 947, 1137 |
| BstV1I | GCAGC | 8 cut(s) | 154, 286, 364, 728, 731, 1123, 1213, 1263 |
| BstX2I | RGATCY | 1 cut(s) | 730 |
| BstYI | RGATCY | 1 cut(s) | 730 |
| BstZI | CGGCCG | 1 cut(s) | 942 |
| BsuRI | GGCC | 6 cut(s) | 5, 32, 281, 461, 944, 1161 |
| BtgI | CCRYGG | 3 cut(s) | 846, 900, 945 |
| BtgZI | GCGATG | 2 cut(s) | 619, 931 |
| BtrI | CACGTC | 2 cut(s) | 128, 994 |
| BtsCI | GGATG | 4 cut(s) | 75, 236, 352, 1286 |
| BtsI | GCAGTG | 2 cut(s) | 33, 1083 |
| BtsIMutI | CAGTG | 3 cut(s) | 33, 1083, 1303 |
| BtuMI | TCGCGA | 2 cut(s) | 482, 916 |
| BveI | ACCTGC | 2 cut(s) | 685, 1107 |
| Cac8I | GCNNGC | 3 cut(s) | 843, 914, 1139 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1162 |
| CciI | TCATGA | 1 cut(s) | 153 |
| CciNI | GCGGCCGC | 1 cut(s) | 942 |
| CfoI | GCGC | 5 cut(s) | 511, 724, 1024, 1116, 1137 |
| Cfr10I | RCCGGY | 2 cut(s) | 295, 1018 |
| Cfr13I | GGNCC | 7 cut(s) | 4, 280, 340, 563, 780, 904, 1159 |
| Cfr42I | CCGCGG | 1 cut(s) | 948 |
| Csp6I | GTAC | 4 cut(s) | 270, 601, 857, 1054 |
| CviAII | CATG | 6 cut(s) | 146, 154, 473, 512, 763, 962 |
| CviQI | GTAC | 4 cut(s) | 270, 601, 857, 1054 |
| DdeI | CTNAG | 5 cut(s) | 209, 284, 935, 1194, 1317 |
| DinI | GGCGCC | 1 cut(s) | 1023 |
| DpnI | GATC | 5 cut(s) | 533, 732, 758, 954, 974 |
| DpnII | GATC | 5 cut(s) | 531, 730, 756, 952, 972 |
| EaeI | YGGCCR | 1 cut(s) | 942 |
| EagI | CGGCCG | 1 cut(s) | 942 |
| Eam1104I | CTCTTC | 5 cut(s) | 327, 354, 921, 960, 1094 |
| EarI | CTCTTC | 5 cut(s) | 327, 354, 921, 960, 1094 |
| Ecl136II | GAGCTC | 1 cut(s) | 819 |
| EclXI | CGGCCG | 1 cut(s) | 942 |
| Eco105I | TACGTA | 1 cut(s) | 492 |
| Eco130I | CCWWGG | 1 cut(s) | 432 |
| Eco24I | GRGCYC | 1 cut(s) | 821 |
| Eco47I | GGWCC | 4 cut(s) | 340, 563, 780, 904 |
| Eco47III | AGCGCT | 1 cut(s) | 723 |
| Eco52I | CGGCCG | 1 cut(s) | 942 |
| Eco53kI | GAGCTC | 1 cut(s) | 819 |
| Eco57I | CTGAAG | 1 cut(s) | 23 |
| EcoICRI | GAGCTC | 1 cut(s) | 819 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1159 |
| EcoRII | CCWGG | 3 cut(s) | 239, 342, 1155 |
| EcoT14I | CCWWGG | 1 cut(s) | 432 |
| EcoT22I | ATGCAT | 1 cut(s) | 238 |
| EcoT38I | GRGCYC | 1 cut(s) | 821 |
| EgeI | GGCGCC | 1 cut(s) | 1023 |
| EheI | GGCGCC | 1 cut(s) | 1023 |
| ErhI | CCWWGG | 1 cut(s) | 432 |
| FaeI | CATG | 6 cut(s) | 149, 157, 476, 515, 766, 965 |
| FatI | CATG | 6 cut(s) | 145, 153, 472, 511, 762, 961 |
| FauI | CCCGC | 2 cut(s) | 850, 1256 |
| FbaI | TGATCA | 1 cut(s) | 952 |
| FokI | GGATG | 4 cut(s) | 62, 223, 359, 1273 |
| FriOI | GRGCYC | 1 cut(s) | 821 |
| FspBI | CTAG | 5 cut(s) | 407, 725, 734, 1142, 1151 |
| GlaI | GCGC | 5 cut(s) | 510, 723, 1023, 1115, 1136 |
| HaeII | RGCGCY | 2 cut(s) | 725, 1025 |
| HaeIII | GGCC | 6 cut(s) | 5, 32, 281, 461, 944, 1161 |
| HapII | CCGG | 3 cut(s) | 257, 296, 1019 |
| HhaI | GCGC | 5 cut(s) | 511, 724, 1024, 1116, 1137 |
| Hin1I | GRCGYC | 1 cut(s) | 1022 |
| Hin1II | CATG | 6 cut(s) | 149, 157, 476, 515, 766, 965 |
| Hin6I | GCGC | 5 cut(s) | 509, 722, 1022, 1114, 1135 |
| HinP1I | GCGC | 5 cut(s) | 509, 722, 1022, 1114, 1135 |
| HincII | GTYRAC | 2 cut(s) | 131, 390 |
| HindII | GTYRAC | 2 cut(s) | 131, 390 |
| HindIII | AAGCTT | 1 cut(s) | 446 |
| HinfI | GANTC | 7 cut(s) | 20, 157, 403, 517, 625, 664, 1271 |
| HpaI | GTTAAC | 1 cut(s) | 390 |
| HpaII | CCGG | 3 cut(s) | 257, 296, 1019 |
| HphI | GGTGA | 4 cut(s) | 457, 559, 845, 1182 |
| Hpy166II | GTNNAC | 4 cut(s) | 131, 390, 775, 1056 |
| Hpy188I | TCNGA | 4 cut(s) | 85, 957, 1206, 1335 |
| Hpy188III | TCNNGA | 7 cut(s) | 55, 154, 481, 521, 734, 915, 1151 |
| Hpy8I | GTNNAC | 4 cut(s) | 131, 390, 775, 1056 |
| Hpy99I | CGWCG | 1 cut(s) | 356 |
| HpyAV | CCTTC | 7 cut(s) | 47, 472, 751, 1037, 1041, 1066, 1182 |
| HpyCH4III | ACNGT | 3 cut(s) | 274, 644, 1236 |
| HpyCH4IV | ACGT | 5 cut(s) | 127, 252, 491, 855, 993 |
| HpyCH4V | TGCA | 6 cut(s) | 224, 236, 606, 694, 1111, 1308 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 506, 947, 1003, 1028, 1226 |
| HpyF3I | CTNAG | 5 cut(s) | 209, 284, 935, 1194, 1317 |
| HpySE526I | ACGT | 5 cut(s) | 127, 252, 491, 855, 993 |
| Hsp92I | GRCGYC | 1 cut(s) | 1022 |
| Hsp92II | CATG | 6 cut(s) | 149, 157, 476, 515, 766, 965 |
| HspAI | GCGC | 5 cut(s) | 509, 722, 1022, 1114, 1135 |
| KasI | GGCGCC | 1 cut(s) | 1021 |
| Ksp22I | TGATCA | 1 cut(s) | 952 |
| KspAI | GTTAAC | 1 cut(s) | 390 |
| KspI | CCGCGG | 1 cut(s) | 948 |
| Kzo9I | GATC | 5 cut(s) | 531, 730, 756, 952, 972 |
| LmnI | GCTCC | 2 cut(s) | 709, 1321 |
| Lsp1109I | GCAGC | 8 cut(s) | 154, 286, 364, 728, 731, 1123, 1213, 1263 |
| LweI | GCATC | 6 cut(s) | 159, 211, 211, 245, 487, 1295 |
| MaeI | CTAG | 5 cut(s) | 407, 725, 734, 1142, 1151 |
| MaeII | ACGT | 5 cut(s) | 127, 252, 491, 855, 993 |
| MaeIII | GTNAC | 1 cut(s) | 1188 |
| MalI | GATC | 5 cut(s) | 533, 732, 758, 954, 974 |
| MbiI | CCGCTC | 2 cut(s) | 941, 1324 |
| MboI | GATC | 5 cut(s) | 531, 730, 756, 952, 972 |
| MboII | GAAGA | 9 cut(s) | 241, 344, 371, 562, 607, 938, 977, 982, 1111 |
| MflI | RGATCY | 1 cut(s) | 730 |
| MhlI | GDGCHC | 1 cut(s) | 821 |
| MluCI | AATT | 2 cut(s) | 426, 616 |
| Mly113I | GGCGCC | 1 cut(s) | 1022 |
| MlyI | GAGTC | 1 cut(s) | 634 |
| Mph1103I | ATGCAT | 1 cut(s) | 238 |
| MseI | TTAA | 7 cut(s) | 389, 444, 450, 678, 1167, 1238, 1257 |
| MslI | CAYNNNNRTG | 1 cut(s) | 185 |
| MspA1I | CMGCKG | 3 cut(s) | 421, 586, 947 |
| MspI | CCGG | 3 cut(s) | 257, 296, 1019 |
| MspR9I | CCNGG | 4 cut(s) | 241, 258, 344, 1157 |
| Mva1269I | GAATGC | 1 cut(s) | 238 |
| MvaI | CCWGG | 3 cut(s) | 241, 344, 1157 |
| MvnI | CGCG | 5 cut(s) | 482, 638, 916, 947, 1137 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 506, 947, 1003, 1028, 1226 |
| NarI | GGCGCC | 1 cut(s) | 1022 |
| NciI | CCSGG | 1 cut(s) | 258 |
| NdeII | GATC | 5 cut(s) | 531, 730, 756, 952, 972 |
| NlaIII | CATG | 6 cut(s) | 149, 157, 476, 515, 766, 965 |
| NlaIV | GGNNCC | 3 cut(s) | 123, 438, 1023 |
| NmuCI | GTSAC | 1 cut(s) | 1188 |
| NotI | GCGGCCGC | 1 cut(s) | 942 |
| NruI | TCGCGA | 2 cut(s) | 482, 916 |
| NsiI | ATGCAT | 1 cut(s) | 238 |
| OliI | CACNNNNGTG | 1 cut(s) | 185 |
| PagI | TCATGA | 1 cut(s) | 153 |
| PaqCI | CACCTGC | 1 cut(s) | 685 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 381 |
| PctI | GAATGC | 1 cut(s) | 238 |
| PfeI | GAWTC | 6 cut(s) | 20, 157, 403, 517, 664, 1271 |
| PfoI | TCCNGGA | 2 cut(s) | 239, 342 |
| PleI | GAGTC | 1 cut(s) | 633 |
| PluTI | GGCGCC | 1 cut(s) | 1025 |
| PpsI | GAGTC | 1 cut(s) | 633 |
| Ppu21I | YACGTR | 2 cut(s) | 253, 492 |
| Psp124BI | GAGCTC | 1 cut(s) | 821 |
| Psp6I | CCWGG | 3 cut(s) | 239, 342, 1155 |
| PspGI | CCWGG | 3 cut(s) | 239, 342, 1155 |
| PspN4I | GGNNCC | 3 cut(s) | 123, 438, 1023 |
| PspPI | GGNCC | 7 cut(s) | 4, 280, 340, 563, 780, 904, 1159 |
| PstI | CTGCAG | 1 cut(s) | 1113 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1162 |
| PsuI | RGATCY | 1 cut(s) | 730 |
| PvuII | CAGCTG | 2 cut(s) | 421, 586 |
| RruI | TCGCGA | 2 cut(s) | 482, 916 |
| RsaI | GTAC | 4 cut(s) | 271, 602, 858, 1055 |
| RsaNI | GTAC | 4 cut(s) | 270, 601, 857, 1054 |
| RseI | CAYNNNNRTG | 1 cut(s) | 185 |
| SacI | GAGCTC | 1 cut(s) | 821 |
| SacII | CCGCGG | 1 cut(s) | 948 |
| SaqAI | TTAA | 7 cut(s) | 389, 444, 450, 678, 1167, 1238, 1257 |
| Sau3AI | GATC | 5 cut(s) | 531, 730, 756, 952, 972 |
| Sau96I | GGNCC | 7 cut(s) | 4, 280, 340, 563, 780, 904, 1159 |
| ScaI | AGTACT | 1 cut(s) | 602 |
| SchI | GAGTC | 1 cut(s) | 634 |
| ScrFI | CCNGG | 4 cut(s) | 241, 258, 344, 1157 |
| SduI | GDGCHC | 1 cut(s) | 821 |
| SfaNI | GCATC | 6 cut(s) | 159, 211, 211, 245, 487, 1295 |
| SfcI | CTRYAG | 2 cut(s) | 15, 1109 |
| SfoI | GGCGCC | 1 cut(s) | 1023 |
| Sfr303I | CCGCGG | 1 cut(s) | 948 |
| SgrAI | CRCCGGYG | 1 cut(s) | 1018 |
| SgrBI | CCGCGG | 1 cut(s) | 948 |
| SinI | GGWCC | 4 cut(s) | 340, 563, 780, 904 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 185 |
| SnaBI | TACGTA | 1 cut(s) | 492 |
| Sse9I | AATT | 2 cut(s) | 426, 616 |
| SspDI | GGCGCC | 1 cut(s) | 1021 |
| SspMI | CTAG | 5 cut(s) | 407, 725, 734, 1142, 1151 |
| SstI | GAGCTC | 1 cut(s) | 821 |
| StyD4I | CCNGG | 4 cut(s) | 239, 256, 342, 1155 |
| StyI | CCWWGG | 1 cut(s) | 432 |
| TaaI | ACNGT | 3 cut(s) | 274, 644, 1236 |
| TaiI | ACGT | 5 cut(s) | 130, 255, 494, 858, 996 |
| TaqI | TCGA | 5 cut(s) | 164, 198, 522, 574, 768 |
| TasI | AATT | 2 cut(s) | 426, 616 |
| TatI | WGTACW | 1 cut(s) | 600 |
| TauI | GCSGC | 5 cut(s) | 281, 539, 944, 947, 950 |
| TfiI | GAWTC | 6 cut(s) | 20, 157, 403, 517, 664, 1271 |
| Tru1I | TTAA | 7 cut(s) | 389, 444, 450, 678, 1167, 1238, 1257 |
| Tru9I | TTAA | 7 cut(s) | 389, 444, 450, 678, 1167, 1238, 1257 |
| TscAI | CASTG | 3 cut(s) | 33, 1083, 1303 |
| TseFI | GTSAC | 1 cut(s) | 1188 |
| TseI | GCWGC | 8 cut(s) | 142, 299, 377, 716, 719, 1111, 1226, 1251 |
| Tsp45I | GTSAC | 1 cut(s) | 1188 |
| TspDTI | ATGAA | 3 cut(s) | 540, 602, 978 |
| TspGWI | ACGGA | 1 cut(s) | 863 |
| TspRI | CASTG | 3 cut(s) | 33, 1083, 1303 |
| VpaK11BI | GGWCC | 4 cut(s) | 340, 563, 780, 904 |
| XapI | RAATTY | 1 cut(s) | 426 |
| XbaI | TCTAGA | 2 cut(s) | 733, 1150 |
| XspI | CTAG | 5 cut(s) | 407, 725, 734, 1142, 1151 |
| ZrmI | AGTACT | 1 cut(s) | 602 |
| Zsp2I | ATGCAT | 1 cut(s) | 238 |