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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 377 |
| AccB1I | GGYRCC | 1 cut(s) | 377 |
| AccII | CGCG | 3 cut(s) | 134, 178, 338 |
| AciI | CCGC | 6 cut(s) | 82, 134, 336, 401, 474, 479 |
| AclWI | GGATC | 3 cut(s) | 262, 294, 440 |
| AcsI | RAATTY | 1 cut(s) | 435 |
| AfaI | GTAC | 1 cut(s) | 379 |
| AgsI | TTSAA | 1 cut(s) | 35 |
| AjnI | CCWGG | 2 cut(s) | 380, 502 |
| AluBI | AGCT | 3 cut(s) | 239, 398, 499 |
| AluI | AGCT | 3 cut(s) | 239, 398, 499 |
| AlwI | GGATC | 3 cut(s) | 262, 294, 440 |
| Ama87I | CYCGRG | 1 cut(s) | 261 |
| ApoI | RAATTY | 1 cut(s) | 435 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 435 |
| Asp718I | GGTACC | 1 cut(s) | 377 |
| AspS9I | GGNCC | 1 cut(s) | 384 |
| AsuHPI | GGTGA | 1 cut(s) | 440 |
| AvaI | CYCGRG | 1 cut(s) | 261 |
| AvaII | GGWCC | 1 cut(s) | 384 |
| BanI | GGYRCC | 1 cut(s) | 377 |
| BccI | CCATC | 2 cut(s) | 4, 181 |
| BceAI | ACGGC | 2 cut(s) | 342, 487 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 38, 72 |
| BciT130I | CCWGG | 2 cut(s) | 382, 504 |
| BciVI | GTATCC | 1 cut(s) | 67 |
| BfaI | CTAG | 1 cut(s) | 123 |
| BfuI | GTATCC | 1 cut(s) | 67 |
| BisI | GCNGC | 2 cut(s) | 83, 336 |
| BlsI | GCNGC | 2 cut(s) | 84, 337 |
| Bme1390I | CCNGG | 2 cut(s) | 382, 504 |
| Bme18I | GGWCC | 1 cut(s) | 384 |
| BmeT110I | CYCGRG | 1 cut(s) | 261 |
| BmgT120I | GGNCC | 1 cut(s) | 384 |
| BmiI | GGNNCC | 1 cut(s) | 379 |
| BmrFI | CCNGG | 2 cut(s) | 382, 504 |
| BmsI | GCATC | 1 cut(s) | 208 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 101, 133 |
| BsaJI | CCNNGG | 3 cut(s) | 69, 282, 363 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 473, 503 |
| Bse118I | RCCGGY | 1 cut(s) | 530 |
| BseBI | CCWGG | 2 cut(s) | 382, 504 |
| BseDI | CCNNGG | 3 cut(s) | 69, 282, 363 |
| Bsh1236I | CGCG | 3 cut(s) | 134, 178, 338 |
| Bsh1285I | CGRYCG | 1 cut(s) | 98 |
| BshNI | GGYRCC | 1 cut(s) | 377 |
| BsiEI | CGRYCG | 1 cut(s) | 98 |
| BsiHKCI | CYCGRG | 1 cut(s) | 261 |
| BsiSI | CCGG | 1 cut(s) | 531 |
| BsoBI | CYCGRG | 1 cut(s) | 261 |
| Bsp143I | GATC | 3 cut(s) | 267, 286, 445 |
| Bsp68I | TCGCGA | 1 cut(s) | 178 |
| BspACI | CCGC | 6 cut(s) | 82, 134, 336, 401, 474, 479 |
| BspFNI | CGCG | 3 cut(s) | 134, 178, 338 |
| BspLI | GGNNCC | 1 cut(s) | 379 |
| BspPI | GGATC | 3 cut(s) | 262, 294, 440 |
| BspT107I | GGYRCC | 1 cut(s) | 377 |
| BsrFI | RCCGGY | 1 cut(s) | 530 |
| BssAI | RCCGGY | 1 cut(s) | 530 |
| BssECI | CCNNGG | 3 cut(s) | 69, 282, 363 |
| BssMI | GATC | 3 cut(s) | 267, 286, 445 |
| BssT1I | CCWWGG | 1 cut(s) | 363 |
| Bst2UI | CCWGG | 2 cut(s) | 382, 504 |
| Bst4CI | ACNGT | 1 cut(s) | 96 |
| BstDEI | CTNAG | 1 cut(s) | 78 |
| BstFNI | CGCG | 3 cut(s) | 134, 178, 338 |
| BstKTI | GATC | 3 cut(s) | 270, 289, 448 |
| BstMBI | GATC | 3 cut(s) | 267, 286, 445 |
| BstMCI | CGRYCG | 1 cut(s) | 98 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 65, 114, 184, 326, 335 |
| BstNI | CCWGG | 2 cut(s) | 382, 504 |
| BstSCI | CCNGG | 2 cut(s) | 380, 502 |
| BstUI | CGCG | 3 cut(s) | 134, 178, 338 |
| BstX2I | RGATCY | 1 cut(s) | 267 |
| BstYI | RGATCY | 1 cut(s) | 267 |
| BsuI | GTATCC | 1 cut(s) | 67 |
| BtgZI | GCGATG | 1 cut(s) | 159 |
| BtuMI | TCGCGA | 1 cut(s) | 178 |
| Cfr10I | RCCGGY | 1 cut(s) | 530 |
| Cfr13I | GGNCC | 1 cut(s) | 384 |
| CseI | GACGC | 1 cut(s) | 327 |
| Csp6I | GTAC | 1 cut(s) | 378 |
| CviAII | CATG | 2 cut(s) | 165, 214 |
| CviQI | GTAC | 1 cut(s) | 378 |
| DdeI | CTNAG | 1 cut(s) | 78 |
| DpnI | GATC | 3 cut(s) | 269, 288, 447 |
| DpnII | GATC | 3 cut(s) | 267, 286, 445 |
| EciI | GGCGGA | 1 cut(s) | 494 |
| Eco130I | CCWWGG | 1 cut(s) | 363 |
| Eco47I | GGWCC | 1 cut(s) | 384 |
| Eco88I | CYCGRG | 1 cut(s) | 261 |
| EcoRI | GAATTC | 1 cut(s) | 435 |
| EcoRII | CCWGG | 2 cut(s) | 380, 502 |
| EcoT14I | CCWWGG | 1 cut(s) | 363 |
| ErhI | CCWWGG | 1 cut(s) | 363 |
| FaeI | CATG | 2 cut(s) | 168, 217 |
| FaiI | YATR | 5 cut(s) | 166, 215, 251, 468, 516 |
| FatI | CATG | 2 cut(s) | 164, 213 |
| FauI | CCCGC | 1 cut(s) | 467 |
| Fnu4HI | GCNGC | 2 cut(s) | 83, 336 |
| Fsp4HI | GCNGC | 2 cut(s) | 83, 336 |
| FspBI | CTAG | 1 cut(s) | 123 |
| GluI | GCNGC | 2 cut(s) | 83, 336 |
| HapII | CCGG | 1 cut(s) | 531 |
| HgaI | GACGC | 1 cut(s) | 327 |
| Hin1II | CATG | 2 cut(s) | 168, 217 |
| HincII | GTYRAC | 1 cut(s) | 342 |
| HindII | GTYRAC | 1 cut(s) | 342 |
| HinfI | GANTC | 2 cut(s) | 144, 169 |
| HpaII | CCGG | 1 cut(s) | 531 |
| HphI | GGTGA | 1 cut(s) | 440 |
| Hpy166II | GTNNAC | 1 cut(s) | 342 |
| Hpy188I | TCNGA | 2 cut(s) | 286, 420 |
| Hpy188III | TCNNGA | 6 cut(s) | 99, 177, 203, 263, 494, 543 |
| Hpy8I | GTNNAC | 1 cut(s) | 342 |
| HpyAV | CCTTC | 3 cut(s) | 98, 250, 425 |
| HpyCH4III | ACNGT | 1 cut(s) | 96 |
| HpyCH4IV | ACGT | 1 cut(s) | 64 |
| HpyCH4V | TGCA | 2 cut(s) | 199, 217 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 65, 114, 184, 326, 335 |
| HpyF3I | CTNAG | 1 cut(s) | 78 |
| HpySE526I | ACGT | 1 cut(s) | 64 |
| Hsp92II | CATG | 2 cut(s) | 168, 217 |
| KpnI | GGTACC | 1 cut(s) | 381 |
| Kzo9I | GATC | 3 cut(s) | 267, 286, 445 |
| LmnI | GCTCC | 1 cut(s) | 403 |
| LpnPI | CCDG | 6 cut(s) | 367, 394, 453, 489, 516, 544 |
| LweI | GCATC | 1 cut(s) | 208 |
| MaeI | CTAG | 1 cut(s) | 123 |
| MaeII | ACGT | 1 cut(s) | 64 |
| MalI | GATC | 3 cut(s) | 269, 288, 447 |
| MboI | GATC | 3 cut(s) | 267, 286, 445 |
| MboII | GAAGA | 1 cut(s) | 265 |
| MflI | RGATCY | 1 cut(s) | 267 |
| MluCI | AATT | 7 cut(s) | 30, 41, 54, 194, 369, 435, 508 |
| MlyI | GAGTC | 1 cut(s) | 178 |
| MnlI | CCTC | 3 cut(s) | 64, 199, 292 |
| MroXI | GAANNNNTTC | 1 cut(s) | 435 |
| MspI | CCGG | 1 cut(s) | 531 |
| MspR9I | CCNGG | 2 cut(s) | 382, 504 |
| MvaI | CCWGG | 2 cut(s) | 382, 504 |
| MvnI | CGCG | 3 cut(s) | 134, 178, 338 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 65, 114, 184, 326, 335 |
| NdeII | GATC | 3 cut(s) | 267, 286, 445 |
| NlaIII | CATG | 2 cut(s) | 168, 217 |
| NlaIV | GGNNCC | 1 cut(s) | 379 |
| NmeAIII | GCCGAG | 3 cut(s) | 94, 98, 250 |
| NruI | TCGCGA | 1 cut(s) | 178 |
| PdmI | GAANNNNTTC | 1 cut(s) | 435 |
| PfeI | GAWTC | 1 cut(s) | 144 |
| PfoI | TCCNGGA | 1 cut(s) | 502 |
| PkrI | GCNGC | 2 cut(s) | 84, 337 |
| PleI | GAGTC | 1 cut(s) | 177 |
| PpsI | GAGTC | 1 cut(s) | 177 |
| Psp6I | CCWGG | 2 cut(s) | 380, 502 |
| PspGI | CCWGG | 2 cut(s) | 380, 502 |
| PspN4I | GGNNCC | 1 cut(s) | 379 |
| PspPI | GGNCC | 1 cut(s) | 384 |
| PsuI | RGATCY | 1 cut(s) | 267 |
| RruI | TCGCGA | 1 cut(s) | 178 |
| RsaI | GTAC | 1 cut(s) | 379 |
| RsaNI | GTAC | 1 cut(s) | 378 |
| SatI | GCNGC | 2 cut(s) | 83, 336 |
| Sau3AI | GATC | 3 cut(s) | 267, 286, 445 |
| Sau96I | GGNCC | 1 cut(s) | 384 |
| SchI | GAGTC | 1 cut(s) | 178 |
| ScrFI | CCNGG | 2 cut(s) | 382, 504 |
| SetI | ASST | 6 cut(s) | 67, 210, 241, 400, 417, 501 |
| SfaNI | GCATC | 1 cut(s) | 208 |
| SinI | GGWCC | 1 cut(s) | 384 |
| Sse9I | AATT | 7 cut(s) | 30, 41, 54, 194, 369, 435, 508 |
| SsiI | CCGC | 6 cut(s) | 82, 134, 336, 401, 474, 479 |
| SspMI | CTAG | 1 cut(s) | 123 |
| StyD4I | CCNGG | 2 cut(s) | 380, 502 |
| StyI | CCWWGG | 1 cut(s) | 363 |
| TaaI | ACNGT | 1 cut(s) | 96 |
| TaiI | ACGT | 1 cut(s) | 67 |
| TaqI | TCGA | 2 cut(s) | 98, 202 |
| TasI | AATT | 7 cut(s) | 30, 41, 54, 194, 369, 435, 508 |
| TauI | GCSGC | 2 cut(s) | 85, 338 |
| TfiI | GAWTC | 1 cut(s) | 144 |
| TspDTI | ATGAA | 1 cut(s) | 266 |
| VpaK11BI | GGWCC | 1 cut(s) | 384 |
| XapI | RAATTY | 1 cut(s) | 435 |
| XmnI | GAANNNNTTC | 1 cut(s) | 435 |
| XspI | CTAG | 1 cut(s) | 123 |