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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 5 cut(s) | 33, 107, 440, 630, 648 |
| AclWI | GGATC | 3 cut(s) | 533, 573, 586 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 38, 39, 121 |
| AgsI | TTSAA | 2 cut(s) | 497, 523 |
| AjnI | CCWGG | 2 cut(s) | 474, 504 |
| AluBI | AGCT | 3 cut(s) | 142, 175, 221 |
| AluI | AGCT | 3 cut(s) | 142, 175, 221 |
| Alw26I | GTCTC | 2 cut(s) | 296, 373 |
| AlwI | GGATC | 3 cut(s) | 533, 573, 586 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 376 |
| Ama87I | CYCGRG | 1 cut(s) | 606 |
| AoxI | GGCC | 1 cut(s) | 126 |
| ApeKI | GCWGC | 6 cut(s) | 104, 221, 256, 621, 624, 644 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 345, 377 |
| AspLEI | GCGC | 1 cut(s) | 261 |
| AspS9I | GGNCC | 1 cut(s) | 274 |
| AsuC2I | CCSGG | 1 cut(s) | 583 |
| AvaI | CYCGRG | 1 cut(s) | 606 |
| AvaII | GGWCC | 1 cut(s) | 274 |
| BaeGI | GKGCMC | 1 cut(s) | 346 |
| BamHI | GGATCC | 1 cut(s) | 578 |
| BbvCI | CCTCAGC | 1 cut(s) | 297 |
| BbvI | GCAGC | 6 cut(s) | 91, 208, 268, 633, 636, 656 |
| BccI | CCATC | 1 cut(s) | 557 |
| BciT130I | CCWGG | 2 cut(s) | 476, 506 |
| BcnI | CCSGG | 1 cut(s) | 583 |
| BcoDI | GTCTC | 2 cut(s) | 296, 373 |
| BfaI | CTAG | 2 cut(s) | 306, 500 |
| BfmI | CTRYAG | 1 cut(s) | 642 |
| BisI | GCNGC | 8 cut(s) | 105, 222, 257, 441, 622, 625, 645, 648 |
| BlsI | GCNGC | 8 cut(s) | 106, 223, 258, 442, 623, 626, 646, 649 |
| Bme1390I | CCNGG | 3 cut(s) | 476, 506, 583 |
| Bme18I | GGWCC | 1 cut(s) | 274 |
| BmeT110I | CYCGRG | 1 cut(s) | 606 |
| BmgT120I | GGNCC | 1 cut(s) | 274 |
| BmiI | GGNNCC | 1 cut(s) | 580 |
| BmrFI | CCNGG | 3 cut(s) | 476, 506, 583 |
| BmrI | ACTGGG | 1 cut(s) | 48 |
| BmuI | ACTGGG | 1 cut(s) | 48 |
| Bpu10I | CCTNAGC | 2 cut(s) | 138, 297 |
| BpuMI | CCSGG | 1 cut(s) | 583 |
| Bsa29I | ATCGAT | 1 cut(s) | 231 |
| BsaJI | CCNNGG | 2 cut(s) | 474, 553 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 38, 39, 121 |
| Bse1I | ACTGG | 3 cut(s) | 43, 60, 276 |
| Bse3DI | GCAATG | 2 cut(s) | 282, 465 |
| BseBI | CCWGG | 2 cut(s) | 476, 506 |
| BseCI | ATCGAT | 1 cut(s) | 231 |
| BseDI | CCNNGG | 2 cut(s) | 474, 553 |
| BseGI | GGATG | 1 cut(s) | 549 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 38, 39, 121 |
| BseMI | GCAATG | 2 cut(s) | 282, 465 |
| BseMII | CTCAG | 2 cut(s) | 288, 368 |
| BseNI | ACTGG | 3 cut(s) | 43, 60, 276 |
| BseRI | GAGGAG | 1 cut(s) | 314 |
| BseSI | GKGCMC | 1 cut(s) | 346 |
| BseXI | GCAGC | 6 cut(s) | 91, 208, 268, 633, 636, 656 |
| BseYI | CCCAGC | 1 cut(s) | 288 |
| BshFI | GGCC | 1 cut(s) | 128 |
| BshVI | ATCGAT | 1 cut(s) | 231 |
| BsiHKCI | CYCGRG | 1 cut(s) | 606 |
| BsiSI | CCGG | 1 cut(s) | 582 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 38, 39, 121 |
| BsmAI | GTCTC | 2 cut(s) | 296, 373 |
| BsnI | GGCC | 1 cut(s) | 128 |
| BsoBI | CYCGRG | 1 cut(s) | 606 |
| Bsp1286I | GDGCHC | 1 cut(s) | 346 |
| Bsp143I | GATC | 2 cut(s) | 538, 578 |
| BspACI | CCGC | 5 cut(s) | 33, 107, 440, 630, 648 |
| BspANI | GGCC | 1 cut(s) | 128 |
| BspCNI | CTCAG | 2 cut(s) | 289, 369 |
| BspDI | ATCGAT | 1 cut(s) | 231 |
| BspLI | GGNNCC | 1 cut(s) | 580 |
| BspMAI | CTGCAG | 1 cut(s) | 646 |
| BspPI | GGATC | 3 cut(s) | 533, 573, 586 |
| BspQI | GCTCTTC | 1 cut(s) | 450 |
| BsrDI | GCAATG | 2 cut(s) | 282, 465 |
| BsrI | ACTGG | 3 cut(s) | 43, 60, 276 |
| BssECI | CCNNGG | 2 cut(s) | 474, 553 |
| BssMI | GATC | 2 cut(s) | 538, 578 |
| BssT1I | CCWWGG | 1 cut(s) | 553 |
| Bst2UI | CCWGG | 2 cut(s) | 476, 506 |
| Bst6I | CTCTTC | 1 cut(s) | 450 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 669 |
| BstC8I | GCNNGC | 4 cut(s) | 177, 294, 419, 619 |
| BstDEI | CTNAG | 3 cut(s) | 138, 297, 377 |
| BstF5I | GGATG | 1 cut(s) | 549 |
| BstHHI | GCGC | 1 cut(s) | 261 |
| BstKTI | GATC | 2 cut(s) | 541, 581 |
| BstMAI | GTCTC | 2 cut(s) | 296, 373 |
| BstMBI | GATC | 2 cut(s) | 538, 578 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 101, 293, 669 |
| BstNI | CCWGG | 2 cut(s) | 476, 506 |
| BstNSI | RCATGY | 1 cut(s) | 350 |
| BstSCI | CCNGG | 3 cut(s) | 474, 504, 581 |
| BstSFI | CTRYAG | 1 cut(s) | 642 |
| BstSLI | GKGCMC | 1 cut(s) | 346 |
| BstV1I | GCAGC | 6 cut(s) | 91, 208, 268, 633, 636, 656 |
| BstX2I | RGATCY | 2 cut(s) | 538, 578 |
| BstYI | RGATCY | 2 cut(s) | 538, 578 |
| Bsu15I | ATCGAT | 1 cut(s) | 231 |
| BsuRI | GGCC | 1 cut(s) | 128 |
| BsuTUI | ATCGAT | 1 cut(s) | 231 |
| BtsCI | GGATG | 1 cut(s) | 549 |
| Cac8I | GCNNGC | 4 cut(s) | 177, 294, 419, 619 |
| CaiI | CAGNNNCTG | 1 cut(s) | 376 |
| CfoI | GCGC | 1 cut(s) | 261 |
| Cfr13I | GGNCC | 1 cut(s) | 274 |
| ClaI | ATCGAT | 1 cut(s) | 231 |
| CviAII | CATG | 4 cut(s) | 187, 347, 394, 484 |
| DdeI | CTNAG | 3 cut(s) | 138, 297, 377 |
| DpnI | GATC | 2 cut(s) | 540, 580 |
| DpnII | GATC | 2 cut(s) | 538, 578 |
| Eam1104I | CTCTTC | 1 cut(s) | 450 |
| EarI | CTCTTC | 1 cut(s) | 450 |
| Eco130I | CCWWGG | 1 cut(s) | 553 |
| Eco147I | AGGCCT | 1 cut(s) | 128 |
| Eco32I | GATATC | 1 cut(s) | 229 |
| Eco47I | GGWCC | 1 cut(s) | 274 |
| Eco88I | CYCGRG | 1 cut(s) | 606 |
| EcoRII | CCWGG | 2 cut(s) | 474, 504 |
| EcoRV | GATATC | 1 cut(s) | 229 |
| EcoT14I | CCWWGG | 1 cut(s) | 553 |
| EcoT22I | ATGCAT | 1 cut(s) | 489 |
| ErhI | CCWWGG | 1 cut(s) | 553 |
| FaeI | CATG | 4 cut(s) | 190, 350, 397, 487 |
| FatI | CATG | 4 cut(s) | 186, 346, 393, 483 |
| FauI | CCCGC | 2 cut(s) | 40, 100 |
| Fnu4HI | GCNGC | 8 cut(s) | 105, 222, 257, 441, 622, 625, 645, 648 |
| FokI | GGATG | 1 cut(s) | 536 |
| Fsp4HI | GCNGC | 8 cut(s) | 105, 222, 257, 441, 622, 625, 645, 648 |
| FspBI | CTAG | 2 cut(s) | 306, 500 |
| GlaI | GCGC | 1 cut(s) | 260 |
| GluI | GCNGC | 8 cut(s) | 105, 222, 257, 441, 622, 625, 645, 648 |
| GsaI | CCCAGC | 1 cut(s) | 292 |
| HaeIII | GGCC | 1 cut(s) | 128 |
| HapII | CCGG | 1 cut(s) | 582 |
| HhaI | GCGC | 1 cut(s) | 261 |
| Hin1II | CATG | 4 cut(s) | 190, 350, 397, 487 |
| Hin6I | GCGC | 1 cut(s) | 259 |
| HinP1I | GCGC | 1 cut(s) | 259 |
| HincII | GTYRAC | 3 cut(s) | 6, 26, 569 |
| HindII | GTYRAC | 3 cut(s) | 6, 26, 569 |
| HindIII | AAGCTT | 1 cut(s) | 140 |
| HinfI | GANTC | 1 cut(s) | 373 |
| HpaII | CCGG | 1 cut(s) | 582 |
| Hpy166II | GTNNAC | 3 cut(s) | 6, 26, 569 |
| Hpy188I | TCNGA | 2 cut(s) | 253, 378 |
| Hpy188III | TCNNGA | 4 cut(s) | 244, 520, 576, 608 |
| Hpy8I | GTNNAC | 3 cut(s) | 6, 26, 569 |
| Hpy99I | CGWCG | 1 cut(s) | 365 |
| HpyAV | CCTTC | 4 cut(s) | 139, 582, 607, 648 |
| HpyCH4V | TGCA | 3 cut(s) | 179, 487, 644 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 101, 293, 669 |
| HpyF3I | CTNAG | 3 cut(s) | 138, 297, 377 |
| Hsp92II | CATG | 4 cut(s) | 190, 350, 397, 487 |
| HspAI | GCGC | 1 cut(s) | 259 |
| Kzo9I | GATC | 2 cut(s) | 538, 578 |
| LguI | GCTCTTC | 1 cut(s) | 450 |
| LmnI | GCTCC | 2 cut(s) | 253, 660 |
| Lsp1109I | GCAGC | 6 cut(s) | 91, 208, 268, 633, 636, 656 |
| MaeI | CTAG | 2 cut(s) | 306, 500 |
| MaeIII | GTNAC | 2 cut(s) | 52, 449 |
| MalI | GATC | 2 cut(s) | 540, 580 |
| MboI | GATC | 2 cut(s) | 538, 578 |
| MboII | GAAGA | 1 cut(s) | 437 |
| MflI | RGATCY | 2 cut(s) | 538, 578 |
| MhlI | GDGCHC | 1 cut(s) | 346 |
| MlyI | GAGTC | 1 cut(s) | 382 |
| MmeI | TCCRAC | 2 cut(s) | 231, 411 |
| MnlI | CCTC | 9 cut(s) | 11, 67, 118, 145, 292, 328, 391, 448, 508 |
| Mph1103I | ATGCAT | 1 cut(s) | 489 |
| MseI | TTAA | 3 cut(s) | 462, 678, 688 |
| MspI | CCGG | 1 cut(s) | 582 |
| MspR9I | CCNGG | 3 cut(s) | 476, 506, 583 |
| MvaI | CCWGG | 2 cut(s) | 476, 506 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 101, 293, 669 |
| NciI | CCSGG | 1 cut(s) | 583 |
| NdeII | GATC | 2 cut(s) | 538, 578 |
| NlaIII | CATG | 4 cut(s) | 190, 350, 397, 487 |
| NlaIV | GGNNCC | 1 cut(s) | 580 |
| NmuCI | GTSAC | 1 cut(s) | 52 |
| NsiI | ATGCAT | 1 cut(s) | 489 |
| NspI | RCATGY | 1 cut(s) | 350 |
| PceI | AGGCCT | 1 cut(s) | 128 |
| PciSI | GCTCTTC | 1 cut(s) | 450 |
| PfoI | TCCNGGA | 1 cut(s) | 581 |
| PkrI | GCNGC | 8 cut(s) | 106, 223, 258, 442, 623, 626, 646, 649 |
| PleI | GAGTC | 1 cut(s) | 381 |
| PpsI | GAGTC | 1 cut(s) | 381 |
| Psp6I | CCWGG | 2 cut(s) | 474, 504 |
| PspFI | CCCAGC | 1 cut(s) | 288 |
| PspGI | CCWGG | 2 cut(s) | 474, 504 |
| PspN4I | GGNNCC | 1 cut(s) | 580 |
| PspPI | GGNCC | 1 cut(s) | 274 |
| PstI | CTGCAG | 1 cut(s) | 646 |
| PstNI | CAGNNNCTG | 1 cut(s) | 376 |
| PsuI | RGATCY | 2 cut(s) | 538, 578 |
| SapI | GCTCTTC | 1 cut(s) | 450 |
| SaqAI | TTAA | 3 cut(s) | 462, 678, 688 |
| SatI | GCNGC | 8 cut(s) | 105, 222, 257, 441, 622, 625, 645, 648 |
| Sau3AI | GATC | 2 cut(s) | 538, 578 |
| Sau96I | GGNCC | 1 cut(s) | 274 |
| SchI | GAGTC | 1 cut(s) | 382 |
| ScrFI | CCNGG | 3 cut(s) | 476, 506, 583 |
| SduI | GDGCHC | 1 cut(s) | 346 |
| SfcI | CTRYAG | 1 cut(s) | 642 |
| SinI | GGWCC | 1 cut(s) | 274 |
| SseBI | AGGCCT | 1 cut(s) | 128 |
| SsiI | CCGC | 5 cut(s) | 33, 107, 440, 630, 648 |
| SspMI | CTAG | 2 cut(s) | 306, 500 |
| StuI | AGGCCT | 1 cut(s) | 128 |
| StyD4I | CCNGG | 3 cut(s) | 474, 504, 581 |
| StyI | CCWWGG | 1 cut(s) | 553 |
| TaqI | TCGA | 4 cut(s) | 165, 231, 245, 363 |
| TauI | GCSGC | 2 cut(s) | 443, 650 |
| Tru1I | TTAA | 3 cut(s) | 462, 678, 688 |
| Tru9I | TTAA | 3 cut(s) | 462, 678, 688 |
| TseFI | GTSAC | 1 cut(s) | 52 |
| TseI | GCWGC | 6 cut(s) | 104, 221, 256, 621, 624, 644 |
| Tsp45I | GTSAC | 1 cut(s) | 52 |
| TspDTI | ATGAA | 1 cut(s) | 121 |
| VpaK11BI | GGWCC | 1 cut(s) | 274 |
| XceI | RCATGY | 1 cut(s) | 350 |
| XspI | CTAG | 2 cut(s) | 306, 500 |
| Zsp2I | ATGCAT | 1 cut(s) | 489 |